journal
MENU ▼
Read by QxMD icon Read
search

Biotechnology and Bioengineering

journal
https://www.readbyqxmd.com/read/29337367/correlation-of-simulation-finite-element-analysis-to-the-separation-of-intrinsically-magnetic-spores-and-red-blood-cells-using-a-microfluidic-magnetic-deposition-system
#1
Jianxin Sun, L Moore, Wei Xue, James Kim, Maciej Zborowski, Jeffrey J Chalmers
Magnetic separation of cells has been, and continues to be, widely used in a variety of applications, ranging from healthcare diagnostics to detection of food contamination. Typical, these technologies require cells labeled with antibody magnetic particle conjugate and a high magnetic energy gradient created in the flow containing the labeled cells (i.e. a column packed with magnetically inducible material), or dense packing of magnetic particles next to the flow cell. Such designs while creating high magnetic energy gradients, are not amenable to easy, highly detailed, mathematic characterization...
January 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29337363/inhibition-of-apoptosis-using-exosomes-in-chinese-hamster-ovary-cell-culture
#2
Seora Han, Won Jong Rhee
Animal cell culture technology for therapeutic protein production has shown significant improvement over the last few decades. Chinese hamster ovary (CHO) cells have been widely adapted for the production of biopharmaceutical drugs. In the biopharmaceutical industry, it is crucial to develop cell culture media and culturing conditions to achieve the highest productivity and quality. However, CHO cells are significantly affected by apoptosis in the bioreactors, resulting in a substantial decrease in product quantity and quality...
January 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29337346/effective-role-of-medium-supplementation-in-microalgal-lipid-accumulation
#3
Azadeh Fazeli Danesh, Peter Mooij, Sirous Ebrahimi, Robbert Kleerebezem, Mark van Loosdrecht
The present study investigated the interaction between starch and lipid accumulation was in a green microalgae enrichment culture. The objective was to optimize the lipid content by manipulation of the medium in regular batch culture. Two medium designs were evaluated: first a high ortho-P concentration with vitamin supplement (Pi-vitamins supplemented medium), second normal growth medium (control). Both media contained a low amount of nitrogen which was consumed during batch growth in three days. The batch experiments were prolonged continued for another four days with the absence of soluble nitrogen in the medium...
January 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315517/role-of-respiratory-terminal-oxidases-in-the-extracellular-electron-transfer-ability-of-cyanobacteria
#4
Narendran Sekar, Jian Wang, Yan Zhou, Yi Fang, Yajun Yan, Ramaraja P Ramasamy
Cyanobacteria are used as anode catalysts in photo-bioelectrochemical cells to generate electricity in a sustainable, economic and environmental friendly manner using only water and sunlight. Though cyanobacteria (CB) possess unique advantage for solar energy conversion by virtue of its robust photosynthesis, they cannot efficiently perform extracellular electron transfer (EET). The reasons being, unlike dissimilatory metal reducing bacteria (that are usually exploited in microbial fuel cells to generate electricity), (1) CB do not possess any special features on their outer membrane to carry out EET and (2) the electrons generated in photosynthetic electron transport chain are channeled into competing respiratory pathways rather than to the anode...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315494/isotype-dependent-on-column-non-reversible-aggregation-of-monoclonal-antibodies
#5
Monika Farys, Daniel Gibson, Alan Lewis, Will Lewis, Richard Kucia-Tran
Monoclonal antibodies of the IgG2 and IgG4 isotype were found to exhibit an increased propensity for displaying two-peak elution profiles during cation exchange chromatography. In some cases, this two-peak elution profile also resulted in the formation of non-reversible mAb aggregates. Comparison of IgG1, IgG2 and IgG4 molecules with the same variable region reveals that the two-peak behaviour is predominantly mediated by the constant region and most likely the lower CH1, hinge and upper CH2 regions of the mAb...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315487/using-extensional-flow-to-reveal-diverse-aggregation-landscapes-for-three-igg1-molecules
#6
Leon F Willis, Amit Kumar, John Dobson, Nick Bond, David Lowe, Richard Turner, Sheena E Radford, Nikil Kapur, David J Brockwell
Monoclonal antibodies (mAbs) currently dominate the biopharmaceutical sector due to their potency and efficacy against a range of disease targets. These proteinaceous therapeutics are, however, susceptible to unfolding, mis-folding and aggregation by environmental perturbations. Aggregation thus poses an enormous challenge to biopharmaceutical development, production, formulation and storage. Hydrodynamic forces have also been linked to aggregation, but the ability of different flow fields (e.g. shear and extensional flow) to trigger aggregation has remained unclear...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315484/ultra-scale-down-approaches-to-study-the-centrifugal-harvest-for-viral-vaccine-production
#7
Beatrice J Melinek, Sandrine Dessoy, Bernice Wright, Dan G Bracewell, Tarit K Mukhopadhyay
Large scale continuous cell-line cultures promise greater reproducibility and efficacy for the production of influenza vaccines, and adenovirus for gene therapy. This paper seeks to use an existing validated ultra scale-down tool, which is designed to mimic the commercial scale process environment using only millilitres of material, to provide some initial insight into the performance of the harvest step for these processes. The performance of industrial scale centrifugation and subsequent downstream process units is significantly affected by shear...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315481/development-of-a-temperature-responsive-yeast-cell-factory-using-engineered-gal4-as-a-protein-switch
#8
Pingping Zhou, Wenping Xie, Zhen Yao, Yongqiang Zhu, Lidan Ye, Hongwei Yu
Conflict between cell growth and product accumulation is frequently encountered in biosynthesis of secondary metabolites. Herein, a temperature-dependent dynamic control strategy was developed by modifying the GAL regulation system to facilitate two-stage fermentation in yeast. A temperature-sensitive Gal4 mutant Gal4M9 was created by directed evolution, and used as a protein switch in ΔGAL80 yeast. After EGFP-reported validation of its temperature-responsive induction capability, the sensitivity and stringency of this system in multi-gene pathway regulation was tested, using lycopene as an example product...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315477/enrichment-of-high-affinity-subclasses-and-glycoforms-from-serum-derived-igg-using-fc%C3%AE-rs-as-affinity-ligands
#9
Boesch Aw, Kappel Jh, Mahan Ae, Chu Th, Crowley Ar, Osei-Owusu Ny, Alter G, Ackerman Me
As antibodies continue to gain predominance in drug discovery and development pipelines, efforts to control and optimize their activity in vivo have matured to incorporate sophisticated abilities to manipulate engagement of specific Fc binding partners. Such efforts to promote diverse functional outcomes include modulating IgG-Fc affinity for FcγRs to alternatively potentiate or reduce effector functions, such as antibody-dependent cellular cytotoxicity and phagocytosis. While a number of natural and engineered Fc features capable of eliciting variable effector functions have been demonstrated in vitro and in vivo, elucidation of these important functional relationships has taken significant effort through use of diverse genetic, cellular and enzymatic techniques...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315476/consolidated-bioprocessing-of-lignocellulosic-biomass-to-lactic-acid-by-a-synthetic-fungal-bacterial-consortium
#10
Robert Lawrence Shahab, Jeremy S Luterbacher, Simone Brethauer, Michael Hans-Peter Studer
Consolidated bioprocessing (CBP) of lignocellulosic feedstocks to platform chemicals requires complex metabolic processes, which are commonly executed by single genetically engineered microorganisms. Alternatively, synthetic consortia can be employed to compartmentalize the required metabolic functions among different specialized microorganisms as demonstrated in this work for the direct production of lactic acid from lignocellulosic biomass. We composed an artificial cross-kingdom consortium and co-cultivated the aerobic fungus Trichoderma reesei for the secretion of cellulolytic enzymes with facultative anaerobic lactic acid bacteria...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29315475/selective-production-of-decanoic-acid-from-iterative-reversal-of-%C3%AE-oxidation-pathway
#11
Seohyoung Kim, Ramon Gonzalez
Decanoic acid is a valuable compound used as precursor for industrial chemicals, pharmaceuticals and biofuels. Despite efforts to produce it from renewables, only limited achievements have been reported. Here, we report an engineered cell factory able to produce decanoic acid as a major product from glycerol, and abundant and renewable feedstock. We exploit the overlapping chain-length specificity of β-oxidation reversal (r-BOX) and thioesterase enzymes to selectively generate decanoic acid. This was achieved by selecting r-BOX enzymes that support the synthesis of acyl-CoA of up to 10 carbons (thiolase BktB and enoyl-CoA reductase EgTER) and a thioesterase that exhibited high activity towards decanoyl-CoA and longer-chain acyl-CoAs (FadM)...
January 8, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29313898/modulation-of-saturation-and-chain-length-of-fatty-acids-in-saccharomyces-cerevisiae-for-production-of-cocoa-butter-like-lipids
#12
David Bergenholm, Michael Gossing, Yongjun Wei, Verena Siewers, Jens Nielsen
Chain length and degree of saturation plays an important role for the characteristics of various products derived from fatty acids, such as fuels, cosmetics and food additives. The seeds of Theobroma cacao are the source of cocoa butter, a natural lipid of high interest for the food and cosmetics industry. Cocoa butter is rich in saturated fatty acids that are stored in the form of triacylglycerides (TAGs). One of the major TAG species of cocoa butter, consisting of two stearic acid molecules and one oleic acid molecule (stearic acid-oleic acid-stearic acid, sn-SOS), is particularly rare in nature as the saturated fatty acid stearic acid is typically found only in low abundance...
January 4, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29288587/from-lab-to-market-an-integrated-bioprocess-design-approach-for-new-to-nature-biosurfactants-produced-by-starmerella-bombicola
#13
Lisa Van Renterghem, Sophie L K W Roelants, Niki Baccile, Katrijn Uyttersprot, Marie Claire Taelman, Bernd Everaert, Stein Mincke, Sam Ledegen, Sam Debrouwer, Kristel Scholtens, Christian Stevens, Wim Soetaert
Glycolipid microbial biosurfactants, like sophorolipids (SLs), generate high industrial interest as 100% biobased alternatives for traditional surfactants. A well-known success story is the efficient SL producer Starmerella bombicola, which reaches titers well above 200 g/L. Recent engineering attempts have enabled the production of completely new types of molecules by S. bombicola, like the 'non-symmetrical bolaform (nsBola) SLs'. As classic SLs are mostly applied in eco-friendly detergents, the possible use of these bolaform SLs in detergent applications was evaluated by scaling up the production process (150 L) and evaluating the purified product...
December 30, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29288579/a-robust-protocol-for-directed-aryl-sulfotransferase-evolution-towards-the-carbohydrate-building-block-glcnac
#14
Shohana S Islam, Diana M Mate, Ronny Martínez, Felix Jakob, Ulrich Schwaneberg
Bacterial aryl sulfotransferases (AST) utilize p-nitrophenylsulfate (pNPS) as a phenolic donor to sulfurylate typically a phenolic acceptor. Interest in aryl sulfotransferases is growing because of their broad variety of acceptors and cost-effective sulfuryl-donors. For instance, aryl sulfotransferase A (ASTA) from Desulfitobacterium hafniense was recently reported to sulfurylate D-glucose. In this study, a directed evolution protocol was developed and validated for aryl sulfotransferase B (ASTB). Thereby the well-known pNPS quantification system was advanced to operate efficiently as a continuous screening system in 96-well MTP format with a true coefficient of variation of 14...
December 30, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29288576/gold-nanorod-photosensitizer-conjugates-with-glutathione-sensitive-linkages-for-synergistic-cancer-photodynamic-photothermal-therapy
#15
Jongseon Choi, Sangeun Lee, Jeong-Sook Park, So Yeon Kim
Recently, photodynamic therapy (PDT) has been intensively investigated as a useful modality for the treatment of various cancers. In addition, near infrared (NIR) photothermal therapy (PTT) using gold nanocarriers has attracted particular interest as a hyperthermia strategy. In this study, gold nanorod (AuNR)-photosensitizer conjugates with glutathione-sensitive linkages were designed for PDT and PTT. Several kinds of AuNRs with different aspect ratios were synthesized and modified with FA-conjugated block copolymers (FA-PEG-P(Asp)-DHLA) and Chlorine6 (Ce6) as a photosensitizer...
December 30, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29288575/high-titer-oncolytic-measles-production-process-by-integration-of-dielectric-spectroscopy-as-online-monitoring-system
#16
Tanja A Grein, Daniel Loewe, Hauke Dieken, Denise Salzig, Tobias Weidner, Peter Czermak
Oncolytic viruses offer new hope to millions of patients with incurable cancer. One promising class of oncolytic viruses is Measles virus, but its broad administration to cancer patients is currently hampered by the inability to produce the large amounts of virus needed for treatment (1010 -1012 virus particles per dose). Measles virus is unstable, leading to very low virus titers during production. The time of infection and time of harvest are therefore critical parameters in a Measles virus production process, and their optimization requires an accurate online monitoring system...
December 30, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29288574/modeling-and-optimization-of-lipid-accumulation-by-yarrowia-lipolytica-from-glucose-under-nitrogen-depletion-conditions
#17
Carlos Eduardo Robles-Rodríguez, Rafael Muñoz-Tamayo, Carine Bideaux, Nathalie Gorret, Stéphane E Guillouet, Carole Molina-Jouve, Gilles Roux, César Arturo Aceves-Lara
Oleaginous yeasts have been seen as a feasible alternative to produce the precursors of biodiesel due to their capacity to accumulate lipids as triacylglycerol having profiles with high content of unsaturated fatty acids. The yeast Yarrowia lipolytica is a promising microorganism that can produce lipids under nitrogen depletion conditions and excess of the carbon source. However, under these conditions, this yeast also produces citric acid (overflow metabolism) decreasing lipid productivity. This work presents two mathematical models for lipid production by Yarrowia lipolytica from glucose...
December 30, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29280498/precision-porous-templated-scaffolds-of-varying-pore-size-drive-dendritic-cell-activation
#18
Ruying Chen, Hongyan Ma, Lei Zhang, James D Bryers
Scaffold based systems have shown significant potential in modulating immune responses in vivo. While there has been much attention on macrophage interactions with tissue engineered scaffolds for tissue regeneration, fewer studies have looked at the effects of scaffold design on the response of immune cells - i.e., dendritic cells (DCs). Here we present the effects of varying pore size of poly (2-hydroxyethyl methacrylate) (pHEMA) and poly(dimethylsiloxane) (PDMS, silicone) scaffolds on the maturation and in vivo enrichment of DCs...
December 27, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29280481/methanol-independent-induction-in-pichia-pastoris-by-simple-derepressed-overexpression-of-single-transcription-factors
#19
Thomas Vogl, Lukas Sturmberger, Pia C Fauland, Patrick Hyden, Jasmin Elgin Fischer, Christian Schmid, Gerhard G Thallinger, Martina Geier, Anton Glieder
Carbon source regulated promoters are well-studied standard tools for controlling gene expression. Acquiring control over the natural regulation of promoters is important for metabolic engineering and synthetic biology applications. In the commonly used protein production host Komagataella phaffii (Pichia pastoris), methanol-inducible promoters are used because of their tight regulation and exceptional strength. Yet, induction with toxic and flammable methanol can be a considerable safety risk and cannot be applied in many existing fermentation plants...
December 27, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29280480/identification-of-process-conditions-influencing-protein-aggregation-in-chinese-hamster-ovary-cell-culture
#20
Albert Jesuran Paul, René Handrick, Sybille Ebert, Friedemann Hesse
Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitable. A preferable approach is to reduce the level of aggregation during bioprocessing by a careful adjustment of critical process parameters. Recently, new analytical methods enabled characterization of mAb aggregation during bioprocessing of mammalian cells...
December 27, 2017: Biotechnology and Bioengineering
journal
journal
25652
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"