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Folia Biologica (Krakow)

Michal Santocki, A John Morgan, Barbara Plytycz
Stressed earthworms expel coelomic fluid containing several vital cellular and soluble components, thus their post-stress recovery has adaptive value. The present manuscript describes the recovery rates of coelomocytes (amoebocytes and eleocytes) and two fluorophores (riboflavin and 4-methylumbelliferyl β-D-glucuronide, MUG) after experimental extrusion by electrostimulation. Analyses were conducted at time points (from 0.5 hour to 7 weeks) by a combination of cell counts, spectrofluorimetric measurements of riboflavin and MUG, and fluorescence microscopy...
2016: Folia Biologica (Krakow)
Teresa Napiórkowska, Paweł Napiórkowski, Julita Templin
Oligomely is an anomaly manifested in the morphology of spiders (except for deformations of the prosoma and exoskeleton), by the absence of one or more appendages, and in their anatomy by the absence of neuromeres. This study was aimed at determining whether there is a correlation between the absence of a neuromere or its half in the subesophageal ganglia and the volume of the prosoma. Morphometric studies involved oligomelic specimens of Tegenaria atrica with the absence of one walking leg and two walking legs...
2016: Folia Biologica (Krakow)
Łukasz Migdał, Toma Ząbek, Anna Migdał, Stanisław Łapiński, Józef Bieniek, Monika Bugno-Poniewierska
In this study, nutria-specific DNA fragments were amplified with cross-species primers for parts of coat colour genes (MC1R, ASIP, TYRP-1, c-KIT) conserved across a number of species. Twenty-three nutria-specific DNA fragments were generated using cross-species PCR (26.7% amplification success rate). Certain limitations of this approach were encountered, including strong mismatching in the primer binding sites. Sequences of 12 nutria-specific PCR products aligned in the BLASTx option showed homology with known protein sequences...
2016: Folia Biologica (Krakow)
Maria Lalkovicova, Jozef Burda, Miroslava Nemethova, Rastislav Burda, Viera Danielisova
Trimethyltin (TMT) is a toxic substance formerly used as a catalyst in the production of organic substances, as well as in industry and agriculture. TMT poisoning has caused death or severe injury in many dozens of people. The toxicity of TMT is mediated by dose dependent selective damage to the limbic system in humans and other animals, specifically the degeneration of CA1 neurons in the hippocampus. The typical symptoms include memory loss and decreased learning ability. Using knowledge gained in previous studies of global ischaemia, we used delayed postconditioning after TMT intoxication (8 mg/kg i...
2016: Folia Biologica (Krakow)
Marta Kuchta-Gładysz, Ewa Wójcik, Olga Szeleszczuk, Piotr Niedbała, Tomasz Wojnar
The sister chromatid exchange test is regarded as a highly sensitive cytogenetic assay. It measures chromosome sensitivity to particular damage factors and provides information on control and repair mechanism performance. It is instrumental in the early identification of the effects of noxious factors present in the habitat. This investigation was aimed at identifying sister chromatid exchange sites in coypu and rabbit chromosomes, as well as determining the spontaneity of the process by applying different BrdU doses...
2016: Folia Biologica (Krakow)
Agnieszka K Grzegorzewska, Marcin W Lis, Andrzej Sechman
The chicken chorioallantoic membrane (CAM) is used as a model in tests of angiogenesis, the biocompatibility of materials as well as tumor invasive potential. To assess the properties of CAM tissue, the localization of leptin receptor in the CAM, and the mRNA expression of two leptin receptor isoforms, estrogen receptors (ERα and ERβ) and caspases (-1 and -3) in the CAM on embryonic days 12 (E12), 15 (E15) and 18 (E18) were investigated. The leptin receptor was immunolocalized in each structure of the CAM (chorionic epithelium, allantoic epithelium, mesodermal layer and the walls of blood vessels) and did not change among analyzed stages of embryonic development (E12, E15 and E18) and between sexes...
2016: Folia Biologica (Krakow)
Ryszard Galus, Paweł Włodarski, Sławomir Mazur, Krzysztof Włodarski, Aniela Brodzikowska
The frequency of osteocytic lacunae, expressed as mean lacunae number per 1000 μm2 of measured bone, evaluated 65 days post intramuscular implantation of demineralized incisors is higher (1.10 ± 0.19) than in femoral (orthotopic) bone (0.91 ± 0.16). The surface of evaluated bones was measured by means of the "weight of bone picture". These results provide new data on the biology of ectopic bone.
2016: Folia Biologica (Krakow)
Katarzyna Dziewulska, Małgorzata Pilecka-Rapacz, Józef Domagała
The sunfish are classified as an invasive species after their introduction into a new habitat. Milt quality and spermatozoa movement in several sunfish were described. The aim of this study was to determine the basic milt characteristics and spermatozoa motility parameters in pumpkinseed males after using ambient water and a standardized solution to examine the parameters in mimicked natural conditions and in controlled conditions allowing interspecies data comparisons. Nine spermatozoa motility parameters were traced using computer-assisted sperm analysis (CASA) after activation with 30 mM NaCl and ambient water at 20°C...
2016: Folia Biologica (Krakow)
Regina Daszkiewicz, Magdalena Szymoniak, Łukasz Gąsior, Zbigniew Polański
Mouse prophase oocytes isolated from antral follicles may possess two alternative types of chromatin configuration: NSN configuration represents more dispersed chromatin and is characteristic mainly for growing oocytes whereas SN configuration, attained upon oocyte growth, comprises more condensed chromatin with a significant fraction concentrated around the nucleolus. Importantly, fully grown oocytes isolated from antral follicles represent a non-homogenous population in which some oocytes posses NSN-type and others SN-type of chromatin conformation...
2016: Folia Biologica (Krakow)
Lidia Kołodziejczyk, Wojciech Podraza, Bolesław Gonet, Ewa Dzika, Danuta I Kosik-Bogacka
The aim of this study was to determine the effect of an extremely low-frequency magnetic field (ELFMF) on the production of liver fluke larvae in a parasite-host system: Fasciola hepatica--Galba truncatula. Both F. hepatica eggs and F. hepatica-infected snails were exposed to an ELFMF (50 Hz, 2.0 mT) for 14 days and 36 days, respectively. F. hepatica-infected snails were divided into 4 groups, 10 specimens each. The snails of groups I and II were infected with F. hepatica larvae--miracidia obtained from control cultures, while the snails of groups III and IV were infected with miracidia reared from eggs that had been incubated in an ELFMF...
2016: Folia Biologica (Krakow)
Patrycja Zagata, Magdalena Greczek-Stachura, Sebastian Tarcz, Maria Rautian
Paramecium bursaria (Ehrenberg 1831), a freshwater ciliate, typically harbors hundreds of green algal symbionts inside the cell. The aim of present study was the molecular identification of newly analyzed P. bursaria symbionts. The second aspect of the present survey was testing a hypothesis whether endosymbionts prefer the specified syngen of the host, and the specified geographical distribution. Ten strains of endosymbionts isolated from strains of P. bursaria originating from different geographical locations were studied...
2016: Folia Biologica (Krakow)
Katarzyna Stadnicka, Magdalena Bodnar, Andrzej Marszałek, Anna Bajek, Tomasz Drewa, Grazyna Płucienniczak, Luiza Chojnacka-Puchta, Violetta Cecuda-Adamczewska, Aleksandra Dunisławska, Marek Bednarczyk
This work shows the usefulness of chicken oviduct epithelial cells (COEC) in evaluating the efficacy of non-viral expression vectors carrying human therapeutic genes. Secondly, an efficient source of progenitor COEC for in vitro studies is described. Within the distal part of the oviduct, weak to moderate expression of a trans membrane glycoprotein (CD44) was observed. Single cells presenting only weak expression of CD44 were found in magnum sections. in vitro cultured oviduct cells originating from the distal oviduct were suitable for subculturing and showed a stable proliferation potential up to the 2nd passage...
2016: Folia Biologica (Krakow)
Ewa Pecka-Kiełb, Jolanta Bujok, Dorota Miśta, Bozena Króliczewska, Justyna Górecka, Wojciech Zawadzki
The aim of this study was to evaluate wild boar (Sus scrofa scrofa) caecal and colon products of microbial activity including short chain fatty acids (SCFA), ammonia and methane concentrations. The in vitro method was applied to caecal and colon contents after 12 and 24-hour incubation with the substrate (wheat bran), or without any additive (control samples). The pH was also measured in each sample. In samples incubated with the substrate, a lower pH was noted as compared to the control (P < 0.001). In terms of the total SCFA concentration, the hindgut microbial fermentation pattern of wild boar was characterized by a high acetate level, followed by propionate and then butyrate at a ratio of 7:1...
2016: Folia Biologica (Krakow)
Vilmar Machado, Jose Serrano, Jose Galián
The fall armyworm (Spodoptera frugiperda, Noctuidae, Lepidoptera) is one of the most important crop pests in the Americas, causing significant damage to maize, rice and sorghum. The mechanisms that determine its defences against pathogens are particularly relevant for the development of management and control strategies. We used an in silico approach to identify and characterize pathogen response genes (repat) present in different tissue libraries of S. fugiperda. The analyses revealed complete cDNA for nine repat genes; of these, repat15 and repat39 were found in libraries from a specific tissue--the midgut of larvae fed with xenobiotic substances...
2016: Folia Biologica (Krakow)
Grazyna Furgała-Selezniow, Małgorzata Jankun, Roman Kujawa, Joanna Nowosad, Maria Biłas, Dariusz Kucharczyk, Andrzej Skrzypczak
The ontogeny of the digestive tract was studied histologically in burbot, Lota lota L., from hatching to 42 days post-hatch (dph). At hatching, the digestive tract consisted of a straight tube with discernible digestive accessory glands (the liver and the pancreas) dorsally attached to the yolk sac. Most of the yolk sac reserves were consumed during the first 12 days and were completely depleted by 17 dph. The first PAS-positive goblet cells appeared at 6 dph, dispersed within the epithelium of the oesophagus and increasing substantially in number and distribution as development progressed...
2016: Folia Biologica (Krakow)
Jacek Czepiel, Joanna Gdula-Argasińska, Aleksander Garlicki
The implications of circulating essential fatty acids (FA) on the inflammatory risk profile and clinical outcome are still unclear. In order to gain a deeper understanding of the role of polyunsaturated fatty acids (PUFA) in the pathogenesis of acute infection, we analyzed the FA content in red blood cell (RBC) membranes of patients with Clostridium difficile infection (CDI) and controls. We prospectively studied 60 patients including 30 patients with CDI and 30 controls to assess lipid concentrations in erythrocyte membranes using gas chromatography...
2016: Folia Biologica (Krakow)
Malgorzata Prajer
No abstract text is available yet for this article.
2016: Folia Biologica (Krakow)
Marija Stanojević, Srdjan Lopicic, Zorica Jovanovic, Dhruba Pathak, Dragan V Pavlovic, Svetolik Spasic, Vladimir Nedeljkov, Milica Prostran
The effects of Mg2+ on Ni(2+)-induced epileptiform bursting activity and input membrane resistance during this activity of leech Retzius neurons were examined using intracellular recordings. To induce epileptiform activity, 3 mmol/l NiCl2 was added into superfusing Ringer (Ri) saline. To test for dose-dependence of the effects of Mg2+ on the induced epileptiform activity, MgCl2 was added in concentrations from 1 mmol/l to 20 mmol/l Mg2+ to the Ni(2+)-containing Ri saline. Input membrane resistance (IMR) was measured in standard Ri, Ni2+ Ri and 20 mmol/l Mg2+Ni2+ Ri saline...
2015: Folia Biologica (Krakow)
Nazar Shapoval, Vladimir Lukhtanov
In our study we use a 690 bp fragment of the COI gene to analyze a taxon from southern Greece, usually treated as Polyommatus (Agrodiaetus) iphigenia nonacriensis (Brown, 1977). The previous conclusions on taxonomy and nomenclature of P. (A.) iphigenia nonacriensis were not supported by molecular or cytological data, therefore the problem of identity of this taxon has remained unsolved. We found that with respect to COI haplotypes, P. (A.) iphigenia nonacriensis from Greece is similar to the studied populations of P...
2015: Folia Biologica (Krakow)
Maria Rautian, Ewa Przyboś, Alexandra Beliavskaia
Paramecium is one of the most studied genera among ciliates. In particular, it is a model organism for investigation of the sibling species problem (also known as the cryptic species problem), spatial distribution, and its role in speciation. The global distribution of Paramecium species and of sibling species belonging to the P. aurelia species complex (Ciliophora, Protista) still need study, e.g. sampling in some territories has been quite limited, while Europe has been investigated for years with the majority of the P...
2015: Folia Biologica (Krakow)
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