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Bulletin of Entomological Research

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https://www.readbyqxmd.com/read/29110744/biochemical-characterization-a-digestive-trypsin-in-the-midgut-of-large-cabbage-white-butterfly-pieris-brassicae-l-lepidoptera-pieridae
#1
A Sharifloo, A Zibaee, J Jalali Sendi, K Talebi Jahroumi
A comprehensive study on digestive trypsin was undertaken in the larval midgut of Pieris brassicae L. Results of enzymatic compartmentalization showed a significantly higher activity of crude trypsin in the anterior larval midgut rather than posterior-midgut. Using Diethylaminoethyl cellulose fast flow column chromatography a purified trypsin was obtained by specific activity of 21 U mg-1 protein, recovery of 22%, purification fold of 28-fold and molecular weight of 25 kDa. This purified enzyme showed the highest activity at pH 8 and the corresponding temperature of 40°C...
November 7, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29103385/suitability-of-multiple-mediterranean-oak-species-as-a-food-resource-for-reticulitermes-grassei-cl%C3%A3-ment-isoptera-rhinotermitidae
#2
A M Cárdenas, P Gallardo, D Toledo
The subterranean termite Reticulitermes grassei Clément causes lesions in the trunk of Quercus suber L. by constructing feeding galleries, but no information is available regarding other Quercus species from the Mediterranean region. This work aimed to study the suitability of the other main oak species of Mediterranean forests as a food resource for R. grassei. Two experiments, choice and non-choice feeding, were conducted lasting for 15, 30, and 45 days each. In the non-choice experiment, termites were offered one of the following food types: Quercus suber, Quercus ilex L...
November 6, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29081303/molecular-cloning-of-heat-shock-protein-10-hsp10-and-60-hsp60-cdnas-from-galeruca-daurica-coleoptera-chrysomelidae-and-their-expression-analysis
#3
Y Tan, Y Zhang, Z-J Huo, X-R Zhou, B-P Pang
Galeruca daurica (Joannis) is a new outbreak pest in the Inner Mongolia grasslands in northern China. Heat shock protein 10 and 60 (Hsp10 and Hsp60) genes of G. daurica, designated as GdHsp10 and GdHsp60, were cloned by rapid amplification of cDNA ends techniques. Sequence analysis showed that GdHsp10 and GdHsp60 encoded polypeptides of 104 and 573 amino acids, respectively. Sequence alignment and phylogenetic analysis clearly revealed that the amino acids of GdHsp10 and GdHsp60 had high homology and were clustered with other Hsp10 and Hsp60 genes in insects which are highly relative with G...
October 30, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29070094/molecular-systematics-biogeography-and-colony-fusion-in-the-european-dry-wood-termites-kalotermes-spp-blattodea-termitoidae-kalotermitidae
#4
V Scicchitano, F Dedeine, B Mantovani, A Luchetti
European dry-wood termites belong to the genus Kalotermes (Kalotermitidae), one of the two termite genera in Europe. Until the recent description of two new species, Kalotermes italicus in Italy and Kalotermes phoenicae in the eastern Mediterranean area, Kalotermes flavicollis was the only taxon known in this region. The presence of additional entities, suggested by morphological and physiological variation observed in K. flavicollis, was supported by molecular studies revealing four distinct genetic lineages: lineage A, K...
October 26, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29061206/regional-population-differences-of-the-brown-planthopper-nilaparvata-lugens-st%C3%A3-l-in-cambodia-using-genotyping-by-sequencing
#5
M Matsukawa, Mikako Tasaki, Kazuyuki Doi, Kasumi Ito, Kazuhito Kawakita, Toshiharu Tanaka
The brown planthopper Nilaparvata lugens Stål (BPH) can be found year-round in tropical region and causes severe damage to rice. Although there has been documented BPH damage to rice crops in the past decade in Cambodia, the extent of this epidemic is poorly understood. Here, we examined the time variation of BPH population in the abundance of morphotypes in 13 main rice-producing provinces (86 sites) by aspirator method and in the Takeo Province (five sites) by yellow sticky trap method. At least three generations were observed during the 3-month collection period in the rainy growing season...
October 24, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29061199/sulfur-fertilization-increases-defense-metabolites-and-nitrogen-but-decreases-plant-resistance-against-a-host-specific-insect
#6
N A Santos, N C Teixeira, J O S Valim, E F A Almeida, M G A Oliveira, W G Campos
We tested the sulfur-modulated plant resistance hypothesis using potted cabbage (Brassica oleracea var. capitata) plants that were grown without and with increasing levels of sulfur fertilization. Changes in plant chemical traits were assessed and developmental performance of Plutella xylostella, a highly host-specific leaf-chewing insect, was followed. Leaf sulfur concentration gradually increased with growing addition of sulfur in soil; however, there was a generalized saturation response curve, with a plateau phase, for improvements in total leaf nitrogen, defense glucosinolates and insect performance...
October 24, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29061198/biochemical-responses-induced-in-galls-of-three-cynipidae-species-in-oak-trees
#7
I Kot, A Jakubczyk, M Karaś, U Złotek
Gall-making Cynipidae manipulate the leaves of host plant to form galls where offspring find shelter and food. The relationship between oak gallwasp and biochemical mechanisms of galls still requires a better understanding. So, in this research, protein and phenolic compound contents, as well as the activity of antioxidative enzymes and pathogenesis-related (PR) proteins were determined. Galls caused by asexual generation of Cynips quercusfolii L., Neuroterus numismalis (Fourc.) and N. quercusbaccarum L., as a model were used...
October 24, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29039281/inhibitory-effects-of-an-extract-from-non-host-plants-on-physiological-characteristics-of-two-major-cabbage-pests
#8
M Dastranj, E Borzoui, A R Bandani, O L Franco
The diamondback moth (Plutella xylostella) and small white cabbage butterfly (Pieris rapae) are the two main serious pests of cruciferous crops (Brassicaceae) that have developed resistance to chemical control methods. In order to avoid such resistance and also the adverse effects of chemical pesticides on the environment, alternative methods have usually been suggested, including the use of plant enzyme inhibitors. Here, the inhibitory effects of proteinaceous inhibitors extracted from wheat, canola, sesame, bean and triticale were evaluated against the digestive α-amylases, larval growth, development and nutritional indecs of the diamondback moth and small white cabbage butterfly...
October 17, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29037264/cold-acclimation-increases-cold-tolerance-independently-of-diapause-programing-in-the-bean-bug-riptortus-pedestris
#9
J Rozsypal, M Moos, S G Goto
The bean bug (Riptortus pedestris) is a pest of soybeans and other legumes in Japan and other Asian countries. It enters a facultative adult diapause on exposure to short days. While photoperiodism and diapause are well understood in R. pedestris, knowledge of cold tolerance is very limited, as is information on the effect of diapause on cold tolerance. We examined the effect of photoperiod, cold acclimation, and feeding status on cold tolerance in R. pedestris. We found that cold acclimation significantly increased survival at -10°C in both long- and short-day adult R...
October 17, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28969718/last-male-sperm-precedence-in-rhynchophorus-ferrugineus-olivier-observations-in-laboratory-mating-experiments-with-irradiated-males
#10
S Musmeci, S Belvedere, R Sasso, S Arnone, M Cristofaro, P Nobili, A La Marca, A De Biase
The Red Palm Weevil (RPW) Rhynchophorus ferrugineus (Olivier 1790) is an invasive pest from southeastern Asia and Melanesia that in the last 30 years has spread widely in the Middle East and Mediterranean Basin. Its stem-boring larvae cause great damage to several palm species of the Arecaceae family, many of which are economically important for agricultural and ornamental purposes. Therefore, great attention has recently been focused in studying this species to identify sustainable and effective eradication strategies, such as sterile insect technique (SIT)...
October 3, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28958217/laboratory-comparison-of-two-aphelinus-mali-clades-for-control-of-woolly-apple-aphid-from-hebei-province-china
#11
M Su, X Tan, Q Yang, C Zhao, F Wan, H Zhou
Aphelinus mali (Haldeman) is an effective natural enemy of woolly apple aphid (WAA), Eriosoma lanigerum (Hausmann). Previous studies have found that, with WAA from Shandong Province (Qingdao) as the host, there are significant differences in various biological characteristics between a Shandong clade and Liaoning clade of A. mali. The ability of the Shandong clade to control this aphid was significantly higher than that of the Liaoning clade in Shandong Province. In order to determine whether differences were caused by better adaptation of the Shandong parasitoid clade to the population of the host in that province or if it represents a more general fitness of this clade to control the host regardless of location, we compared the same parasitoid clades with hosts from Hebei Province...
September 29, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28950922/oviposition-preferences-of-dengue-vectors-aedes-aegypti-and-aedes-albopictus-in-sri-lanka-under-laboratory-settings
#12
N Gunathilaka, T Ranathunge, L Udayanga, A Wijegunawardena, W Abeyewickreme
Investigations on oviposition behaviour of dengue vectors are critical for effective controlling of vector breeding. Therefore, the aim of the present study was to determine the oviposition behaviour of dengue vectors, Aedes aegypti and Aedes albopictus in Sri Lanka. Batches of 1000 adult mosquitoes (1 : 1, male: female ratio) housed in rearing cages were used for each experimental setup from Ae. aegypti and Ae. albopictus. Oviposition responses with respect to the size of the ovitrap, colours of the ovitrap, water source, sodium chloride (NaCl) concentration and presence/absence of larvae were evaluated by enumerating the number of eggs laid in the ovitraps...
September 27, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28944748/host-location-behavior-of-the-tea-green-leafhopper-empoasca-vitis-g%C3%A3-the-hemiptera-cicadellidae-olfactory-and-visual-effects-on-their-orientation
#13
X Zhang, T Pengsakul, M Tukayo, L Yu, W Fang, D Luo
The tea green leafhopper, Empoasca vitis Göthe, is one of the most serious pests in tea growing areas. This study investigated the roles played by olfaction and vision in host orientation behavior. The compound eye of E. vitis was found to be a photopic eye; few olfactory sensilla were found on the antennae, while abundant gustatory sensilla were recorded on the mouthparts. Three opsin genes (EV_LWop, EV_UVop, EV_Bop) were isolated and found to be mainly expressed in the compound eye compared with other parts of the body...
September 25, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28944746/using-two-sex-life-tables-to-determine-fitness-parameters-of-four-bactrocera-species-diptera-tephritidae-reared-on-a-semi-artificial-diet
#14
W Jaleel, J Yin, D Wang, Y He, L Lu, H Shi
Fruit flies in the genus Bactrocera are global, economically important pests of agricultural food crops. However, basic life history information about these pests, which is vital for designing more effective control methods, is currently lacking. Artificial diets can be used as a suitable replacement for natural host plants for rearing fruit flies under laboratory conditions, and this study reports on the two-sex life-table parameters of four Bactrocera species (Bactrocera correcta, Bactrocera dorsalis, Bactrocera cucurbitae, and Bactrocera tau) reared on a semi-artificial diet comprising corn flour, banana, sodium benzoate, yeast, sucrose, winding paper, hydrochloric acid and water...
September 25, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28929990/microbial-composition-affects-the-performance-of-an-artificial-tephritid-larval-diet
#15
P Rempoulakis, S Sela Saldinger, E Nemny-Lavy, R Pinto, A Birke, D Nestel
The present study investigated the patterns of microorganisms in an artificial larval diet during Dacus ciliatus (Diptera; Tephritidae) larval development. Microbial population contents in the diet of total heterotrophic bacteria, yeast and molds, coliform and lactobacilli, and their dynamics during development, were monitored. Initially, the microbial composition in diet trays failing to produce viable pupae and in trays successfully producing pupae and adult flies was characterized. The failing diet trays contained large populations of lactobacilli that increased during larval development, and low populations of coliforms...
September 20, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28925346/the-effect-of-winter-length-on-duration-of-dormancy-and-survival-of-specialized-herbivorous-rhagoletis-fruit-flies-from-high-elevation-environments-with-acyclic-climatic-variability
#16
J Rull, E Tadeo, R Lasa, M Aluja
Dormancy can be defined as a state of suppressed development allowing insects to cope with adverse conditions and plant phenology. Among specialized herbivorous insects exploiting seasonal resources, diapause frequently evolves as a strategy to adjust to predictable plant seasonal cycles. To cope with acyclic and unpredictable climatic events, it has been found for some insects that a proportion of the population undergoes prolonged dormancy. We compared the response of three species in the Rhagoletis cingulata species group exploiting plants differing in fruiting phenology from environments varying in frequency and timing of acyclic climatic catastrophic events (frost during flowering and fruit set) and varying also in the time of the onset of the rainy season...
September 19, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28925339/improving-the-artificial-diet-for-adult-of-seven-spotted-ladybird-beetle-coccinella-septempunctata-l-coleoptera-coccinellidae-with-orthogonal-design
#17
Y Cheng, J R Zhi, F L Li, W H Li, Y H Zhou
In this study, an orthogonal array design with 16 factors at two levels (216) was performed to develop an artificial diet rearing the adults of seven spotted ladybird beetle Coccinella septempunctata. The parameters of weight gain and survival rate of adults, preoviposition period, fecundity and hatching rate of diet-fed adults were monitored. The 16 factors were included: pork liver, infant formula, sucrose, olive oil, yolk, corn oil, yeast powder, cholesterol, casein, casein hydrolysate, vitamin powder, 65% juvenile hormone III, protein powder, vitamin E, honey and pumpkin...
September 19, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28925338/foraging-behaviour-of-the-exotic-wasp-vespula-germanica-hymenoptera-vespidae-on-a-native-caterpillar-defoliator
#18
A L Pietrantuono, S Moreyra, M Lozada
Vespula germanica is a social wasp and an opportunistic predator. While foraging, these wasps learn and integrate different kinds of cues. They have successfully invaded many parts of the world, including native Nothofagus and Lophozonia forests located in the Andean-Patagonian region, where they forage on native arthropods. Perzelia arda, a lepidopteron defoliator of Lophozonia obliqua, uses the foliage to hide in and feed on. The purpose of this work is to study whether V. germanica use olfactory cues when foraging on P...
September 19, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28920566/field-evolved-resistance-to-%C3%AE-cyhalothrin-in-the-lady-beetle-eriopis-connexa
#19
P M G Costa, J B Torres, V M Rondelli, R Lira
Natural enemies are exposed to insecticide sprays for herbivorous species and may evolve field resistance to insecticides. Natural enemies selected for resistance in the field, however, are welcome for pest control. The susceptibility of 20 populations of Eriopis connexa from various crop ecosystems to λ-cyhalothrin was tested. Three bioassays were conducted: (i) topical treatment with lethal dose (LD)50 previously determined for populations considered standard for susceptibility (LD50S) and for resistance (LD50R) to λ-cyhalothrin at technical grade; (ii) dose-mortality assay to calculate the LD for populations exhibiting significant survival to the LD50R; and (iii) determination of survival when exposed to dried residues at field rates...
September 18, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28920565/regulatory-functions-of-trehalose-6-phosphate-synthase-in-the-chitin-biosynthesis-pathway-in-tribolium-castaneum-coleoptera-tenebrionidae-revealed-by-rna-interference
#20
Q W Chen, S Jin, L Zhang, Q D Shen, P Wei, Z M Wei, S G Wang, B Tang
RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (TPS), which plays a key role in the synthesis of trehalose and insect development, was cloned in Tribolium castaneum (Herbst) (TcTPS) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that TcTPS is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection...
September 18, 2017: Bulletin of Entomological Research
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