journal
MENU ▼
Read by QxMD icon Read
search

Journal of Immunological Methods

journal
https://www.readbyqxmd.com/read/28341440/optimization-and-testing-of-dried-antibody-tube-the-euroflow-lst-and-pidot-tubes-as-examples
#1
V H J van der Velden, J Flores-Montero, Martin Perez, Marta Martin-Ayuso, Oliver Crespo, Elena Blanco, Tomas Kalina, Jan Philippé, Carolien Bonroy, Maaike de Bie, Jeroen Te Marvelde, Cristina Teodosio, Alba Corral Mateos, Veronika Kanderová, Mirjam van der Burg, Dennis Van Hoof, Jacques J M van Dongen, Alberto Orfao
Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the primary immune deficiency orientation tube (PIDOT). Both tubes were evaluated on normal and/or on patient samples, comparing the mean fluorescence intensity of specific lymphocyte populations. Our data show that the dried tubes and liquid counterparts give highly comparable staining results, particularly when analyzed in multidimensional plots...
March 21, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28336396/tumor-control-index-as-a-new-tool-to-assess-tumor-growth-in-experimental-animals
#2
William L Corwin, Hakimeh Ebrahimi-Nik, Stephanie M Floyd, Pouya Tavousi, Ion I Mandoiu, Pramod K Srivastava
Measurement of tumor diameters, tumor volumes, or area under the curve has been traditionally used to quantitate and compare tumor growth curves in immune competent as well as immune-compromised mice and rats. Here, using tumor growth data from a large number of mice challenged with live tumor cells, we describe the use of a new composite parameter, Tumor Control Index (TCI) as an alternative method to do the same. This index, comprised of three distinct values, the Tumor Progression Score, Tumor Rejection Score, and Tumor Stability Score, provides a complete picture of nearly every aspect of tumor growth in large numbers of animals, can be deduced automatically from tumor diameter or volume data, and can be used to compare several groups of animals in different experiments...
March 20, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28336395/human-immune-compartment-comparisons-optimization-of-proliferative-assays-for-blood-and-gut-t-lymphocytes
#3
Jeffrey Dock, Lance Hultin, Patricia Hultin, Julie Elliot, Otto O Yang, Peter A Anton, Beth D Jamieson, Rita B Effros
The accumulation of peripheral blood late-differentiated memory CD8 T cells with features of replicative (cellular) senescence, including inability to proliferate in vitro, has been extensively studied. Importantly, the abundance of these cells is directly correlated with increased morbidity and mortality in older persons. Of note, peripheral blood contains only 2% of the total body lymphocyte population. By contrast, the gut-associated lymphoid tissue (GALT) is the most extensive lymphoid organ, housing up to 60% of total body lymphocytes, but has never been assessed with respect to senescence profiles...
March 20, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28327345/immunoassays-for-riboflavin-and-flavin-mononucleotide-using-antibodies-specific-to-d-ribitol-and-d-ribitol-5-phosphate
#4
G Ravi, Yeldur P Venkatesh
Riboflavin (vitamin B2), a water-soluble vitamin, plays a key role in maintaining human health. Though, numerous methods have been reported for the determination of total riboflavin (TRF) content in foods and biological samples, very few methods are reported for quantifying riboflavin and its coenzymes [flavin mononucleotide (FMN); flavin adenine dinucleotide (FAD)] individually. Recently, we have demonstrated that antibodies specific to d-ribitol and d-ribitol-5-phosphate also recognize riboflavin and FMN, respectively, and not vice-versa...
March 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28322862/comparison-of-in-house-biotin-avidin-tetanus-igg-enzyme-linked-immunosorbent-assay-elisa-with-gold-standard-in-vivo-mouse-neutralization-test-for-the-detection-of-low-level-antibodies
#5
Cemile Sonmez, Nilay Çoplu, Ayşegul Gozalan, Lutfu Akin, Berrin Esen
BACKGROUND: Detection of anti-tetanus antibody levels is necessary for both determination of the immune status of individuals and also for planning preventive measures. ELISA is the preferred test among in vitro tests however it can be affected by the cross reacting antibodies. A previously developed in-house ELISA test was found not reliable for the antibody levels ≤1.0IU/ml. A new method was developed to detect low antibody levels correctly. The aim of the present study was to compare the results of the newly developed in-house biotin-avidin tetanus IgG ELISA test with the in vivo mouse neutralization test, for the antibody levels ≤1...
March 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28322863/deep-immune-profiling-by-mass-cytometry-links-human-t-and-nk-cell-differentiation-and-cytotoxic-molecule-expression-patterns
#6
Bertram Bengsch, Takuya Ohtani, Ramin Sedaghat Herati, Niels Bovenschen, Kyong-Mi Chang, E John Wherry
The elimination of infected or tumor cells by direct lysis is a key T and NK cell effector function. T and NK cells can kill target cells by coordinated secretion of cytotoxic granules containing one or both pore-forming proteins, perforin and granulysin and combinations of granzyme (Gzm) family effector proteases (in humans: Gzm A, B, K, M and H). Understanding the pattern of expression of cytotoxic molecules and the relationship to different states of T and NK cells may have direct relevance for immune responses in autoimmunity, infectious disease and cancer...
March 17, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28300540/detection-of-potassium-channel-kir4-1-antibodies-in-multiple-sclerosis-patients
#7
Fabiana Marnetto, Paola Valentino, Marzia Caldano, Antonio Bertolotto
The presence of KIR4.1 antibodies has been proposed to be a characteristic of Multiple Sclerosis (MS). This could have a significant impact on disease management. However, the validation of the initial findings has failed till date. Conflicting results have been attributed to difficulties in isolating the lower-glycosylated (LG) KIR4.1 expressed in oligodendrocytes, the putative target antigen of autoantibodies. The aim of this study is to verify the presence of KIR4.1 antibodies in MS patients, by independently replicating the originally-described procedure...
March 11, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28288790/establishment-of-the-first-who-international-standard-for-etanercept-a-tnf-receptor-ii-fc-fusion-protein-report-of-an-international-collaborative-study
#8
Meenu Wadhwa, Chris Bird, Paula Dilger, Peter Rigsby, Haiyan Jia, Marie Emmanuelle Behr Gross
Etanercept, a recombinant human tumor necrosis factor (TNF) receptor Fc fusion protein is an effective treatment option in adults with rheumatoid arthritis, ankylosing spondylitis, psoriatic arthritis or plaque psoriasis and paediatrics with juvenile idiotypic arthritis and plaque psoriasis. Patent expiration in Europe and intense development of various etanercept products worldwide triggered a need for an international reference standard to facilitate determination of biological activity. Therefore, three candidate preparations of etanercept were lyophilized and evaluated in a multi-centre collaborative study comprising twenty eight laboratories from 15 countries for their suitability to serve as an international standard for the bioactivity of TNF receptor II Fc fusion proteins (international nonproprietary name, Etanercept)...
March 10, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28283408/development-and-evaluation-of-an-enzyme-linked-immunosorbent-assay-for-the-detection-of-antibodies-to-a-common-urogenital-derivative-of-chlamydia-trachomatis-plasmid-encoded-pgp3
#9
Catherine E Winstanley, Kyle H Ramsey, Peter Marsh, Ian N Clarke
BACKGROUND: Urogenital infection with Chlamydia trachomatis is the most commonly diagnosed sexually transmitted infection in the developed world. Accurate measurement and therefore understanding the seroprevalence of urogenital C. trachomatis infections requires a rigorously optimised and validated ELISA. Previous ELISAs based on the C. trachomatis plasmid-encoded protein, PGP3, have been described but lack standardisation and critical controls or use a less common PGP3 as the capture antigen...
March 7, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28274838/quantitative-comparison-of-human-intestinal-mononuclear-leukocyte-isolation-techniques-for-flow-cytometric-analyses
#10
R R C E Schreurs, A Drewniak, R Bakx, W E Corpeleijn, T H B Geijtenbeek, J B van Goudoever, M J Bunders
Studies on immune cells derived from the human intestine are needed to understand the pathogenesis of gastrointestinal diseases and to develop novel treatment strategies. Isolation techniques to extract these immune cells from intestinal tissue are largely based on murine studies and comparative data on isolation from human intestine is scarce. In this study we evaluated cell yield, viability, and surface-molecule expression on mononuclear leukocytes, comparing three techniques to obtain a single immune cell suspension from human intestine; low concentrations of either the enzymes Collagenase D or Liberase TL, and enzyme-free mechanical dissociation with the Medimachine...
March 6, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28274836/a-novel-gingival-overgrowth-mouse-model-induced-by-the-combination-of-csa-and-ligature-induced-inflammation
#11
Ai Okanobu, Shinji Matsuda, Mikihito Kajiya, Tsuyoshi Fujita, Mizuho Kittaka, Hideki Shiba, Hidemi Kurihara
Drug-induced gingival overgrowth (DIGO) is a side effect of the enlargement of gingival tissue by phenytoin, nifedipine, and cyclosporine A (CsA). Gingival inflammation has been identified as a key factor that initiates DIGO. However, a sufficient animal model for clarifying the role of inflammation in DIGO has not yet been generated. We herein describe a novel CsA-induced gingival overgrowth mouse model to evaluate the role of inflammation. A ligature was placed around the second molar in maxillae for 7days to induce gingival inflammation, and CsA (50mg/kg/day) was administered to mice during each experimental period...
March 6, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28274837/a-novel-method-for-detection-of-ifn-lambda-3-binding-to-cells-for-quantifying-ifn-lambda-receptor-expression
#12
Deanna M Santer, Gillian E S Minty, Adil Mohamed, Lesley Baldwin, Rakesh Bhat, Michael Joyce, Adrian Egli, D Lorne J Tyrrell, Michael Houghton
Type III interferons (IFN-lambdas) are important antiviral cytokines that also modulate immune responses acting through a unique IFN-λR1/IL-10R2 heterodimeric receptor. Conflicting data has been reported for which cells express the IFN-λR1 subunit and directly respond to IFN-λs. In this study we developed a novel method to measure IFN-λ3 binding to IFN-λR1/IL-10R2 on the surface of cells and relate this to a functional readout of interferon stimulated gene (ISG) activity in various cell lines. We show that Huh7...
March 5, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28274835/clinical-immunogenicity-of-the-d-amino-acid-peptide-therapeutic-etelcalcetide-method-development-challenges-and-anti-drug-antibody-clinical-impact-assessments
#13
Mark A Kroenke, Dohan Weeraratne, Hongjie Deng, Bethlyn Sloey, Raju Subramanian, Benjamin Wu, Michael Serenko, M Benjamin Hock
The immunogenicity risk assessment and bioanalytical strategy for novel therapeutics should account for both unique biophysical properties and potential consequences of immunogenicity. When assessing the immunogenicity risk of etelcalcetide, a peptide agonist of the calcium-sensing receptor, we considered the potential that the d-amino acid 'backbone' and biotransformation of etelcalcetide could allow the drug to act as a hapten. As a consequence, we validated and implemented a surface plasmon resonance immunoassay platform with both etelcalcetide and etelcalcetide-'carrier' surfaces to detect anti-drug antibodies (ADA)...
March 5, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28268194/a-bioluminescent-caspase-1-activity-assay-rapidly-monitors-inflammasome-activation-in-cells
#14
Martha O'Brien, Danielle Moehring, Raúl Muñoz-Planillo, Gabriel Núñez, Justin Callaway, Jenny Ting, Mike Scurria, Tim Ugo, Laurent Bernad, James Cali, Dan Lazar
Inflammasomes are protein complexes induced by diverse inflammatory stimuli that activate caspase-1, resulting in the processing and release of cytokines, IL-1β and IL-18, and pyroptosis, an immunogenic form of cell death. To provide a homogeneous method for detecting caspase-1 activity, we developed a bioluminescent, plate-based assay that combines a substrate, Z-WEHD-aminoluciferin, with a thermostable luciferase in an optimized lytic reagent added directly to cultured cells. Assay specificity for caspase-1 is conferred by inclusion of a proteasome inhibitor in the lytic reagent and by use of a caspase-1 inhibitor to confirm activity...
March 4, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28238798/unambiguous-high-resolution-genotyping-of-human-leukocyte-antigens
#15
Robert A Bradshaw, Paul P J Dunn
We have developed a high resolution sequencing based typing method for genotyping Human Leukocyte Antigens (HLA) over a period of twenty years. The methods are based upon the separation of HLA alleles per locus at the initial amplification to simplify the analysis post-sequencing. The increasing discovery of polymorphism in HLA, manifested in new alleles, has necessitated the continuing development of this method. Here we present methods for the high resolution Sequence Based Typing of HLA-A, B, C (class I) and HLA-DQB1 and DRB1 (class II)...
February 23, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28237707/study-of-fluid-dynamics-reveals-direct-communications-between-lymphatic-vessels-and-venous-blood-vessels-at-lymph-nodes-of-mice
#16
Kazu Takeda, Shiro Mori, Tetsuya Kodama
Cancer cells metastasize to lymph nodes, with distant metastasis resulting in poor prognosis. The role of lymph node metastasis (LNM) in the spread of cancer to distant organs remain incompletely characterized. The visualization of flow dynamics in the lymphatic and blood vessels of MXH10/Mo-lpr/lpr mice, which develop systemic swelling of lymph nodes up to 10mm in diameter, has revealed that lymph nodes have the potential to be a direct source of systemic metastasis. However, it is not known whether these fluid dynamics characteristics are universal phenomena present in other strains of laboratory mice...
February 22, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28216237/development-of-an-anti-ferret-cd4-monoclonal-antibody-for-the-characterisation-of-ferret-t-lymphocytes
#17
Daniel S Layton, Xiaowen Xiao, John D Bentley, Louis Lu, Cameron R Stewart, Andrew G D Bean, Timothy E Adams
The ferret is an established animal model for a number of human respiratory viral infections, such as influenza virus and more recently, Ebola virus. However, a paucity of immunological reagents has hampered the study of cellular immune responses. Here we describe the development and characterisation of a novel monoclonal antibody (mAb) against the ferret CD4 antigen and the characterisation of ferret CD4 T lymphocytes. Recombinant production and purification of the ferret CD4 ectodomain soluble protein allowed hybridoma generation and the generation of a mAb (FeCD4) showing strong binding to ferret CD4 protein and lymphoid cells by flow cytometry...
February 16, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28216236/elimination-of-falsely-reactive-results-in-a-commercially-available-west-nile-virus-igm-capture-enzyme-linked-immunosorbent-assay-by-heterophilic-antibody-blocking-reagents
#18
Harry E Prince, Mary Lapé-Nixon, Tara S Givens, Tiffany Bradshaw, Marek J Nowicki
All sera initially reactive in the Focus Diagnostics West Nile virus IgM capture enzyme-linked immunosorbent assay (WNV IgM ELISA) must be retested with background subtraction to identify falsely-reactive (FR) samples due to antibodies that bind to immunoglobulins of other animal species (heterophilic antibodies). In some settings, such as pre-transplant testing of organ donors, the reporting delay associated with retesting can have an adverse impact on donor procurement and organ placement. We sought to determine if inclusion of heterophilic antibody blockers in assay conjugate could eliminate the nonspecific reactivity of FR samples...
February 16, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28212879/flow-cytometric-based-protocols-for-assessing-anti-mt-2-igg1-reactivity-high-dimensional-data-handling-to-define-predictors-for-clinical-follow-up-of-human-t-cell-leukemia-virus-type-1-infection
#19
Jordana Grazziela Coelho-Dos-Reis, Vanessa Peruhype-Magalhães, Marcelo Antônio Pascoal-Xavier, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Ludmila Melo Cardoso, Juan Jonathan-Gonçalves, Ágata Lopes Ribeiro, Ana Lúcia Borges Starling, João Gabriel Ribas, Denise Utsch Gonçalves, Anna Bárbara de Freitas Carneiro-Proietti, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho
The present work provides an innovative methodological approach to assess the anti-HTLV-1 IgG1 reactivity with practical application in clinical laboratory. Serum from non-infected healthy controls (NI) and HTLV-1-infected patients, categorized as asymptomatic (AS), putatively progressing to HTLV-1 associated myelopathy/tropical spastic paraparesis - HAM/TSP (pHAM) or with clinical diagnosis of HAM/TSP (HT) were assayed in two-parallel flow cytometry platforms, referred as: Fix and Fix&Perm protocols. Operating-characteristics analysis indicated that a single pair of attributes ("serum dilution/cut-off") for Fix and Fix&Perm protocols presented excellent performance for the diagnosis of HTLV-1 infection...
February 16, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28212880/automatic-detection-of-hypoxia-in-renal-tissue-stained-with-hif-1alpha
#20
Aline Rodrigues Buzin, Nayana Damiani Macedo, Isabela Bastos Binotti Abreu De Araujo, Breno Valentim Nogueira, Tadeu Uggere de Andrade, Denise Coutinho Endringer, Dominik Lenz
OBJECTIVE: The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD: 17 images of renal tissues from male rats of Winstar lineage; overall, there were 12.587 objects (cells) in the images for analysis. The acquired images were then analyzed through the free softwares CellProfiler (version 2.1...
February 14, 2017: Journal of Immunological Methods
journal
journal
25441
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"