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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://www.readbyqxmd.com/read/27908779/lipoprotein-lipase-does-not-increase-significantly-in-the-postprandial-plasma
#1
Nobuyoshi Ishiyama, Kouji Sakamaki, Younosuke Shimomura, Kazuhiko Kotani, Kokoro Tsuzaki, Naoki Sakane, Kazuya Miyashita, Isao Fukamachi, Junji Kobayashi, Kimber L Stanhope, Peter J Havel, Keiko Kamachi, Akira Tanaka, Yoshiharu Tokita, Tetsuo Machida, Masami Murakami, Katsuyuki Nakajima
BACKGROUND: Previous reports have shown that lipoprotein lipase (LPL) activity significantly increases in the postprandial plasma associated with the increase of TG-rich lipoproteins. Therefore, we have reexamined those relationships using newly developed LPL assay with the different kinds of food intake. METHODS: Standard meal (n=81), 50g of fat (n=54), 75g of glucose (n=25) and cookie (25g fat and 75g carbohydrate fat) (n=28) were administered in generally healthy volunteers...
November 28, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27890570/the-spectrum-of-cyp21a2-mutations-in-congenital-adrenal-hyperplasia-in-an-indian-cohort
#2
Ragini Khajuria, Rama Walia, Anil Bhansali, Rajendra Prasad
Congenital Adrenal Hyperplasia (CAH) is a common autosomal recessive disorder of the adrenal steroidogenic pathway. The most common form of CAH is due to mutations in CYP21A2 gene. The incidence of mutations in the CYP21A2 gene and the genotype-phenotype correlations vary among different populations. Therefore, the aim of the study was to establish the spectrum of mutations and to evaluate genotype-phenotype correlation in Indian CAH population. Molecular defects were investigated in 110 alleles (55 patients) in the present study...
November 24, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27889429/plasmatic-presepsin-scd14-st-concentrations-in-acute-pyelonephritis-in-adult-patients
#3
Yann-Erick Claessens, Eloise Trabattoni, Sophie Grabar, Laurent Quinquis, Guillaume Der Sahakian, Marine Anselmo, Jeannot Schmidt, Jean-Emmanuel de la Coussaye, Patrick Plaisance, Enrique Casalino, Gilles Potel, François Lecomte, Didier Borderie, Camille Chenevier-Gobeaux
INTRODUCTION: Presepsin (sCD14-ST) is an emerging biomarker for infection. We hypothesized that presepsin could specifically increase during acute pyelonephritis and correlate with severity. METHODS: We compared presepsin values in patients with acute pyelonephritis and controls, and we assessed its capacity to predict bacteraemia and admission in patients. RESULTS: In 312 patients with acute pyelonephritis (median age 33years), presepsin concentrations were higher than in controls (476 vs 200ng/L, p<0...
November 24, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27887960/corticosteroid-binding-globulin-cleavage-may-be-pathogen-dependent-in-bloodstream-infection
#4
Marni A Nenke, John G Lewis, Wayne Rankin, David Shaw, David J Torpy
OBJECTIVE: The process of enzymatic cleavage of high- to low-affinity corticosteroid-binding globulin (haCBG to laCBG) by neutrophil elastase leads to local tissue release of cortisol. Recently Pseudomonas aeruginosa was shown to instigate CBG cleavage with release of free cortisol in vitro. Hence, CBG cleavage with release of anti-inflammatory cortisol in infection may be pathogen-dependent. Our objective was to determine whether haCBG and laCBG levels are altered in infected patients compared with controls, and whether these alterations were particular to causative bacteria...
November 22, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27887959/decreased-plasma-concentrations-of-brain-derived-neurotrophic-factor-in-preeclampsia
#5
Luiza Oliveira Perucci, Érica Leandro Marciano Vieira, Antônio Lúcio Teixeira, Karina Braga Gomes, Luci Maria Dusse, Lirlândia Pires Sousa
BACKGROUND: Preeclampsia (PE) is a disease characterized by excessive maternal inflammatory response. Early studies suggested that brain-derived neurotrophic factor (BDNF) modulates inflammation. The main objective of this study was to investigate BDNF plasma concentrations in PE women and to compare with BDNF concentrations from normotensive pregnant women. We also investigated the association among the plasma concentrations of BDNF and inflammatory mediators, and maternal clinical features...
November 22, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27884754/increased-serum-concentrations-of-asymmetric-dimethylarginine-adma-in-patients-with-early-onset-coronary-artery-disease
#6
Chao Xuan, Zhen-Fang Liu, Qing Wang, Fen-Fen Guo, Xiao Zhang, Guo-Wei He, Li-Min Lun
BACKGROUND: Asymmetric dimethylarginine (ADMA) has been associated with an increased risk of cardiovascular disease. We investigated the role of serum ADMA concentrations in early-onset coronary artery disease (EOCAD). METHODS: Candidates for coronary artery angiography (age<50 y for men and <55 y for women) who met the inclusion criteria were enrolled in this study. Serum concentrations of ADMA were determined using ELISA. Severity of coronary atherosclerosis was estimated by number of diseased vessels...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27884753/cell-free-dna-induced-apoptosis-of-granulosa-cells-by-oxidative-stress
#7
Yichun Guan, Wenjuan Zhang, Xingling Wang, Pengfei Cai, Qi Jia, Wenjie Zhao
BACKGROUND: Cell-free DNA is a DNA fragment that is produced by cell apoptosis which can affect the micro-environment of cell apoptosis. The levels of Cell-free DNA have been associated with successful rate of in vitro fertilization-embryo transfer (IVF-ET) and embryonic development. Our aim is to determine the relationship between cell-free DNA and embryo quality. The mechanisms of cell-free DNA in granulose and the apoptosis will be determined also. METHODS: The study enrolled patients who were undergone IVF for the first time and grouped the patients as pregnant (n=130) and non-pregnant (n=59)...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27884752/quantitative-proteomic-analysis-reveals-that-proteins-required-for-fatty-acid-metabolism-may-serve-as-diagnostic-markers-for-gastric-cancer
#8
Zhen Jiang, Hongchun Shen, Bo Tang, Qin Yu, Xingli Ji, Li Wang
BACKGROUND: Gastric cancer is one of the leading causes of cancer-related deaths worldwide. The sensitivities and specificities of current biomarkers for gastric cancer are insufficient for clinical detection, and new diagnostic tests are therefore urgently required. METHODS: A discovery set of gastric cancer and adjacent normal tissues were analyzed for differentially expressed proteins by labeling of peptide digests with isobaric tag for relative and absolute quantitation (iTRAQ) reagents followed by liquid chromatography-electrospray ionization-tandem mass spectrometry...
November 21, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27876463/re-evaluating-the-sensitivity-of-the-rabbit-infectivity-test-for-treponema-pallidum-in-modern-era
#9
Man-Li Tong, Hui-Lin Zhang, Xiao-Zhen Zhu, Jin-Yi Fan, Kun Gao, Li-Rong Lin, Li-Li Liu, Shu-Lian Li, Hui-Ling Lin, Zhi-Feng Lin, Jian-Jun Niu, Wei-Hong Zheng, Tian-Ci Yang
BACKGROUND: The rabbit infectivity test (RIT) was previously described as a highly-sensitive method for clinically detecting Treponema pallidum. But our primary study indicated this result may have changed in current antibiotics era. METHODS: By inoculating rabbits testis with cerebrospinal fluid (CSF) (n=63) and exudate from hard chancre lesions (n=13), we re-evaluated the sensitivity of RIT in modern era. All isolated T. pallidum strains from the RIT were performed for the strain type based on "CDC subtype/tp0548" method...
November 20, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27876464/m%C3%A3-ller-stem-cell-dependent-retinal-regeneration
#10
REVIEW
Annu Chohan, Usha Singh, Atul Kumar, Jasbir Kaur
Müller Stem cells to treat ocular diseases has triggered enthusiasm across all medical and scientific communities. Recent development in the field of stem cells has widened the prospects of applying cell based therapies to regenerate ocular tissues that have been irreversibly damaged by disease or injury. Ocular tissues such as the lens and the retina are now known to possess cell having remarkable regenerative abilities. Recent studies have shown that the Müller glia, a cell found in all vertebrate retinas, is the primary source of new neurons, and therefore are considered as the cellular basis for retinal regeneration in mammalian retinas...
November 19, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27876462/expression-and-clinicopathological-significance-of-s100-calcium-binding-protein-a2-in-lung-cancer-patients-of-chinese-han-ethnicity
#11
Ting Wang, Yiqian Liang, Asmitananda Thakur, Shuo Zhang, Feng Liu, Hamadhaider Khan, Puyu Shi, Ning Wang, Mingwei Chen, Hui Ren
BACKGROUND: S100 family of calcium-binding proteins plays a significant role in the process of many kinds of tumors, including lung cancer. As an important member of this family, S100 calcium binding protein A2 (S100A2) has been confirmed to be associated with many biological processes, and has an abnormal expression in non-small cell lung cancer (NSCLC). However, the S100A2 status in lung cancer is still controversial and undefined. METHODS: We evaluated the pattern and distribution of S100A2 in 109 cases of lung cancer, including five histological types (47 adenocarcinoma, 46 squamous cell carcinoma, 7 small cell carcinoma, 3 large cell carcinoma, and 6 atypical carcinoid), and 30 cases of paired adjacent normal lung tissues by means of immunohistochemistry...
November 19, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27871845/inter-generational-effects-of-the-in-vitro-maturation-technique-on-pregnancy-outcomes-early-development-and-cognition-of-offspring-in-mouse-model
#12
N Wang, C E Ren, Y Y Lou, F Le, L Y Wang, X Z Liu, Q T Zhan, L N Mao, H Y Lou, F Jin
In vitro maturation (IVM) of oocytes has been a highly successful method for avoiding the occurrence of severe ovarian hyperstimulation syndrome in some patients during in vitro fertilization. However, the safety of the protocol, especially the long-term effects, is still an issue of debate. The current study is to investigate the long-term effects of IVM on mice through two generations and reveal its inter-generational effects as well. The data indicate that the rates of embryo resorption and fetal death in the F1 generation were significantly increased while the newborn survival rate in the F1 and F2 generations were significantly decreased in the IVM group...
November 18, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27871844/an-lc-ms-ms-based-candidate-reference-method-for-the-quantification-of-total-gentamicin-in-human-serum-and-plasma-using-nmr-characterized-calibrator-material
#13
Stephanie Lucha, Judith Taibon, Stephan Pongratz, Christian Geletneky, Erasmus Huber, Christine Wintterle-Roehm, Robert Lang, Stefanie H Grimm, Thomas Duelffer, Kirill Tarasov, Johannes Zander, Michael Vogeser, Uwe Kobold
BACKGROUND: Accurate measurement of gentamicin concentration in serum and plasma is required for therapeutic drug monitoring to ensure appropriate treatment of patients. In this work, we present a validated LC-MS/MS-based candidate reference measurement procedure for total gentamicin quantification to be used for standardization and harmonization of routine assays applied for therapeutic drug monitoring of this compound. Total gentamicin is the sum of the concentrations of five known congeners C1, C1a, C2, C2a and C2b...
November 18, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27871843/considerations-when-using-next-generation-sequencing-for-genetic-diagnosis-of-long-qt-syndrome-in-the-clinical-testing-laboratory
#14
Hyojin Chae, Jiyeon Kim, Gun Dong Lee, Woori Jang, Joonhong Park, Dong Wook Jekarl, Yong Seog Oh, Myungshin Kim, Yonggoo Kim
BACKGROUND: Congenital long-QT syndrome (LQTS) is a potentially lethal cardiac electrophysiologic disorder characterized by QT interval prolongation and T-wave abnormalities. At least 13 LQTS-associated genes have been reported, but the high cost and low throughput of conventional Sanger sequencing has hampered the multi-gene-based LQTS diagnosis in clinical laboratories. METHODS: We developed an NGS (next-generation sequencing)-based targeted gene panel for 13 LQTS genes using the Ion PGM platform, and a cohort of 36 LQTS patients were studied for characterization of analytical performance specifications...
November 18, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27871842/evaluation-of-risk-for-metabolic-syndrome-according-to-the-fasting-insulin-concentration-in-korean-men
#15
Ju Young Jung, Sung Keun Park, Joong-Myung Choi, Hyun Pyo Hong, Young-Jun Choi, Jae-Hong Ryoo
BACKGROUND: As a well-known risk factor for cardiovascular disease, metabolic syndrome (MetS) is an important global health problem due to its high worldwide prevalence. The objective of this study is to determine whether the fasting serum insulin concentration influences future incidence of MetS. METHODS: A total of 14,621 Korean men without MetS participating in a medical health check-up program were followed up from 2005 until 2010. They were divided into 4 groups according to baseline fasting insulin concentrations...
November 18, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27867039/peritoneal-dialysis-effluent-mir-21-and-mir-589-levels-correlate-with-longitudinal-change-in-peritoneal-transport-characteristics
#16
Cheuk-Chun Szeto, Kai-Ming Chow, Bonnie Ching-Ha Kwan, Phyllis Mei-Shan Cheng, Cathy Choi-Wan Luk, Jack Kit-Chung Ng, Man-Ching Law, Chi-Bon Leung, Philip Kam-Tao Li
BACKGROUND: The role of microRNA (miRNA) in peritoneal fibrosis and longitudinal change in transport is uncertain. METHODS: We studied 80 new peritoneal dialysis (PD) patients. Peritoneal transport was determined by standard peritoneal equilibration test (PET) of creatinine at baseline. Based on published literature, PD effluent levels of 10 miRNA targets were quantified. PET and miRNA quantification were repeated one year later in 46 patients. RESULTS: Baseline PD effluent levels of all targets tested had modest but significant correlation with peritoneal transport parameters...
November 17, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27865974/importance-of-the-combined-urinary-procedure-for-the-diagnosis-of-mucopolysaccharidoses
#17
Lucia Zampini, Lucia Padella, Rita Lucia Marchesiello, Lucia Santoro, Chiara Monachesi, Andrea Giovagnoni, Carlo Catassi, Orazio Gabrielli, Giovanni Valentino Coppa, Tiziana Galeazzi
BACKGROUND: Mucopolysaccharidoses are characterized by the accumulation of undegraded glycosaminoglycans in lysosomes in multiple organs and by their excretion in high amounts in urine. The aim of this study is to determine if this simple, reliable and reproducible method is useful for the diagnosis of Mucopolysaccharidoses. METHODS: The study included 2154 normal urine samples and 210 samples from 73 patients affected by different types of Mucopolysaccharidoses...
November 16, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27864099/comparability-of-the-effect-of-storage-time-and-temperature-on-serum-anti-m%C3%A3-llerian-hormone-measurement-between-original-and-modified-enzyme-linked-immunosorbent-assay
#18
Chao-Yan Yue, Chun-Mei Ying
OBJECTIVE: To explore the effect of modified enzyme-linked immunosorbent assay on the AMH results is increased or decreased, and to investigate the effect of storage time and temperature on AMH measurements with and without sample premixing assay buffer using the Kangrun ELISA method. METHOD: Serum AMH concentration were measured by ELISA, consistency between two kits, and comparability between original and the modified assay under different stored conditions were analyzed by Passing-Bablok regression analysis and Bland-Altman bias evaluation...
November 16, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27864101/targeted-exome-sequencing-identifies-novel-compound-heterozygous-mutations-in-p3h1-in-a-fetus-with-osteogenesis-imperfecta-type-viii
#19
Yanru Huang, Libin Mei, Weigang Lv, Haoxian Li, Rui Zhang, Qian Pan, Hu Tan, Jing Guo, Xiaomei Luo, Chen Chen, Desheng Liang, Lingqian Wu
Osteogenesis imperfecta (OI) is a highly clinically and genetically heterogeneous group of disorders. It is difficult to identify severe OI in the perinatal period. Here, a Chinese woman with a suspected history of fetal OI was referred to our institution at 19weeks of gestation, due to ultrasound inspection during antenatal screening, which revealed bulbous metaphyses, short humeri, and short thick bent femora in the fetus. Using targeted exome sequencing of 248 genes known to be involved in skeletal system diseases, we identified novel compound heterozygous mutation in the P3H1 gene in the fetus with OI type VIII: c...
November 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27864100/clinical-relevance-of-combined-anti-mitochondrial-m2-detection-assays-for-primary-biliary-cirrhosis
#20
Eunhee Han, Sung Jin Jo, Hyeyoung Lee, Ae-Ran Choi, Jihyang Lim, Eun-Sun Jung, Eun-Jee Oh
BACKGROUND: Antimitochondrial antibody (AMA) is a specific serologic marker in primary biliary cirrhosis (PBC). The aim of this study was to evaluate the clinical relevance of combined AMA assays. METHODS: Sera were obtained from 79 patients with PBC and 108 patients with other liver disease. They were tested by indirect immunofluorescence (IIF) using rat kidney/stomach tissue and HEp2 cells as substrate, 4 AMA-M2 assays, anti-sp100, and anti-gp210 assays. RESULTS: Using IIF-AMA with cut-off titer of 1:40, the sensitivity and specificity for PBC were 88...
November 15, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
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