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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://www.readbyqxmd.com/read/30114408/influence-of-collection-tubes-during-quantitative-targeted-metabolomics-studies-in-human-blood-samples
#1
Giuseppe Paglia, M Fabiola Del Greco, Baldur B Sigurdsson, Johannes Rainer, Chiara Volani, Andrew A Hicks, Peter P Pramstaller, Sigurdur V Smarason
BACKGROUND: Plasma and serum are the most widely used matrices in clinical studies. However, some variability in absolute concentrations of metabolites are likely to be observed in these collection tubes matrices. METHODS: We analyzed 189 metabolites using the same protocol for quantitative targeted metabolomics (LC-MS/MS AbsoluteIDQ p180 Kit Biocrates) in three types of samples, serum, plasma EDTA and citrate, of 80 subjects from the Cooperative Health Research In South Tyrol cohort (40 healthy elderly and 40 healthy young)...
August 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30114407/not-all-chromosome-aberrations-can-be-detected-by-nipt-in-women-at-advanced-maternal-age-a-multicenter-retrospective-study
#2
Ying-Ping Chen, Ze-Quan He, Ye Shi, Qin Zhou, Zheng-Mao Cai, Bin Yu, Ting Wang
OBJECTIVE: To discuss the detectability of NIPT for pregnant women at advanced maternal age (AMA), and mainly focused on how many fetal abnormalities will be missed by NIPT. METHODS: A total of 4194 women at AMA who accepted cytogenetic prenatal diagnosis were recruited in this study. All the AMA women received amniocentesis at 18-23 weeks. Combined with our detection level of NIPT and literature reports, we evaluated the detectability of NIPT. RESULTS: After cell karyotype analysis, a total of 233 (5...
August 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30114406/measurement-of-apixaban-dabigatran-edoxaban-and-rivaroxaban-in-human-plasma-using-automated-online-solid-phase-extraction-combined-with-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-and-its-comparison-with-coagulation-assays
#3
Joachim Kuhn, Tatjana Gripp, Tobias Flieder, Andreas Hammerschmidt, Doris Hendig, Isabel Faust, Cornelius Knabbe, Ingvild Birschmann
BACKGROUND: Measurement of direct oral anticoagulants (DOACs) concentration in patient blood is essential in special clinical circumstances. METHODS: We developed a fast, selective and sensitive method for simultaneous measurement of DOACs in human plasma consisting of an automated online solid-phase extraction method coupled with ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry (online SPE-UPLC-MS/MS). RESULTS: The calibration curves of all DOACs were linear over the working range (apixaban: 0...
August 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30110608/ultrasensitive-quantification-of-cardiac-troponin-i-by-a-single-molecule-counting-method-analytical-validation-and-biological-features
#4
Alvaro Garcia-Osuna, David Gaze, Margarita Grau-Agramunt, Thomas Morris, Catarina Telha, Amelita Bartolome, Jeffrey J Bishop, Luis Monsalve, Richard Livingston, Joel Estis, Niamh Nolan, Johanna Sandlund, Jordi Ordonez-Llanos
AIM: To evaluate analytical and biological characteristics of the Singulex Clarity® cTnI assay, based upon Single Molecule Counting technology. METHODS: Assay's analytical sensitivity, precision, linearity, hook effect, cross-reactivity or interference by endogenous and exogenous substances, stability, 99th reference percentile [p99th] in EDTA plasma were evaluated in single or multi-site studies. RESULTS: Detection limit was 0.12 ng/L...
August 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30110607/false-positive-screen-test-for-mucopolysaccharidoses-in-healthy-female-newborns
#5
Chiara Monachesi, Lucia Zampini, Lucia Padella, Rita Lucia Marchesiello, Tiziana Galeazzi, Lucia Santoro, Carlo Catassi, Enrico Gasparrini, Virgilio Paolo Carnielli, Nicola Volpi, Agata Fiumara, Daniela Concolino, Rosella Tomanin, Giovanni Valentino Coppa, Orazio Gabrielli
BACKGROUND: In total, 930 urine samples obtained on 2nd and 3rd day from birth have been analyzed for the early diagnosis of Mucopolysaccharidoses. METHODS: Dimethylmethylene blue (DMB) assay and one-dimensional electrophoresis were performed in all urine samples. Agarose gel electrophoresis, before and after treatment with chondroitinase ABC and heparinases, was used for a comprehensive characterization. RESULTS: Out of 930 urine samples 7 showed anomalous electrophoretic pattern; 5 of them had high GAG levels by DMB test...
August 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30107158/culture-characters-genetic-background-estrogen-progesterone-receptor-expression-and-tumorigenic-activities-of-frequently-used-sixteen-endometrial-cancer-cell-lines
#6
Wanglei Qu, Yinling Zhao, Xuan Wang, Yaozhi Qi, Clare Zhou, Ying Hua, Jianqing Hou, Shi-Wen Jiang
BACKGROUND: This study aimed to determine the in vitro and in vivo properties of sixteen frequently used endometrial cancer (EC) cell lines, including the cell proliferation rate, morphology, hormone receptor expression patterns, PTEN, hMLH1 expression, p53 mutation, karyotype, and tumorigenicity in mouse xenograt model. METHODS: Twelve type I (AN3, ECC-1, EN, EN-1, EN-11, HEC-1A, HEC1B, Ishikawa, KLE, MFE-280, MFE-296, MFE-319) and four type II (ARK1, ARK2, HEC-155/180, SPEC-2) endometrial cancer cell lines were studied...
August 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30102898/application-of-ultrasonography-in-the-diagnosis-and-treatment-of-cesarean-scar-pregnancy
#7
REVIEW
Dongmei Liu, Min Yang, Qingqing Wu
The morbidity of cesarean scar pregnancy (CSP) has shown an obvious ascending tendency with the increase of cesarean delivery in China and other countries. The timely diagnosis and treatment of CSP currently relies on medical imaging technology. In this article, we analyzed and compare the imaging methods in diagnosis of CSP. The imaging methods to diagnose CSP include traditional two-dimensional color/power Doppler ultrasound (2D-US), three-dimensional color/power Doppler ultrasound (3D-US), contrast-enhanced ultrasound (CEUS), and Magnetic Resonance Imaging (MRI)...
August 10, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30102897/multicenter-comparison-of-imprecision-at-low-concentrations-of-two-regulatory-approved-high-sensitivity-cardiac-troponin-i-assays
#8
LETTER
Joshua E Raizman, Anna Fuezery, Albert K Y Tsui, Trefor Higgins, Bobbi Lynn Goudreau, Lorna Clark, Stephen A Hill, Peter A Kavsak
No abstract text is available yet for this article.
August 10, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30102896/increased-serum-interleukin-33-concentrations-predict-worse-prognosis-of-aneurysmal-subarachnoid-hemorrhage
#9
Jiangbiao Gong, Yu Zhu, Jianbo Yu, Jing Jin, Mantao Chen, Wenchao Liu, Renya Zhan
BACKGROUND: Interleukin-33 (IL-33) is an inflammatory biomarker. We elucidated the relationship between serum IL-33 concentrations, severity and prognosis in aneurysmal subarachnoid hemorrhage (aSAH). METHODS: We prospectively recruited 175 controls and 175 aSAH patients. Serum IL-33 concentrations were gauged using an enzyme-linked immunosorbent assay. Clinical and radiological severity was assessed by World Federation of Neurological Surgeons (WFNS) scale and modified Fisher grading scale respectively...
August 10, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30098951/investigation-of-transition-ion-ratio-variation-for-liquid-chromatography-tandem-mass-spectrometry-a-case-study-approach
#10
Dustin R Bunch, Adam J McShane, Sihe Wang
BACKGROUND: A transition ion ratio (TIR) is the ratio of one fragment over another from the same precursor and is frequently monitored in liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for analyte identification. The Clinical and Laboratory Standards Institute (CLSI) C50-A guidelines give a static percent allowable TIR deviation based on the TIR level. Anecdotally, we observed failures of these rules for some of our LC-MS/MS assays. We determined what parameters may affect TIRs in a clinical setting and whether TIR variations may be analyte, matrix, instrument service, and/or concentration dependent...
August 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30096318/component-resolved-diagnosis-in-allergic-disease-utility-and-limitations
#11
REVIEW
Angel San Miguel-Rodríguez, Alicia Armentia, Sara Martín-Armentia, Blanca Martín-Armentia, Alfredo Corell, Mª Carmen Lozano Estevan, Irene Iglesias Peinado
Component resolved diagnosis (CRD) is a microarray-based diagnostic solution capable of simultaneously analysing specific IgE antibodies against 112 allergenic components, providing sensitivity patterns for multi-sensitised or complex patients. The CRD is indicated for these patients, especially those with concomitant respiratory and food allergies. This study reivews the method, its utility, limitations, and our experience in allergic diseases with difficult etiologic diagnosis (eosinophilic esophagitis, occupational asthma and drug allergy)...
August 7, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30096317/retrospective-study-on-the-outcomes-of-infantile-tufted-angioma-complicated-by-kasabach-merritt-phenomenon
#12
Hong-Zhao Lei, Bin Sun, Yu-Chun Ma, Miao-Miao Li, Li-Fu Wang, Shi-Wen Jiang, Ran Huo, Chang-Xian Dong
OBJECTIVE: To analyze the clinical characteristics and treatment of pediatric tufted angiomas(TA)complicated by Kasabach-Merritt Phenomenon (KMP). METHOD: A retrospective analysis was conducted on the clinical data and follow-up data of 13 patients diagnosed with TA complicated by KMP. Five male and 8 female patients with an average age of 5.7 months (range, 29 days to 1 year) were treated with surgery between January 2009 and June 2012. According to the size and location of lesions and the degree of thrombocytopenia, complete or subtotal resection was performed...
August 7, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30096316/classified-status-of-smoking-and-quitting-has-different-associations-with-dyslipidemia-in-residents-in-northeast-china
#13
Jikang Shi, Ye Bai, Shuang Qiu, Yong Li, Changgui Kou, Yuchun Tao, Qing Zhen, Yulu Gu, Yaqin Yu, Kaixin Zhang, Yi Cheng, Yawen Liu
BACKGROUND: Various smoking status and high prevalence of dyslipidemia in residents exist in northeast China. However, associations of dyslipidemia with smoking status remain unclear. METHODS: A total of 17,114 participants selected by a multistage stratified cluster random sampling design were enrolled from a cross-sectional study conducted in northeast China. Associations of dyslipidemia with smoking/quitting status (smoking amount, smoking duration, and quitting duration) were investigated using multiple logistic regression...
August 7, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30096315/ectopic-expression-of-factor-viii-in-mscs-and-hepatocytes-derived-from-rdna-targeted-hescs
#14
Qianru Sun, Xionghao Liu, Yong Wu, Wenbin Niu, Panpan Long, Jing Liu, Ming Lei, Youjin Hu, Lingqian Wu, Zhuo Li, Desheng Liang
Hemophilia A is an X-linked recessive bleeding disorder caused by FVIII gene deficiency, which may result in spontaneous joint hemorrhages or life-threatening bleeding. Currently, cell-based gene therapy via ex vivo transduction of transplantable cells with integrating gene-expressing vectors offers an attractive treatment for HA. In present study, we targeted an expression cassette of B-domain-deleted FVIII into the ribosomal DNA (rDNA) locus of human embryonic stem cells (hESCs) by transfection with a nonviral targeting plasmid pHrn...
August 7, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30092170/identification-of-essential-hypertension-biomarkers-in-human-urine-by-non-targeted-metabolomics-based-on-uplc-q-tof-ms
#15
Huan Zhao, Yijia Liu, Zhu Li, Yanqi Song, Xuemeng Cai, Yuechen Liu, Tianpu Zhang, Liu Yang, Lin Li, Shan Gao, Yubo Li, Chunquan Yu
BACKGROUND: In recent years, using metabolomics technology to study hypertension has made some progress. However, in actual clinical studies, there are few studies on hypertension related metabonomics with human urine as samples. In this study, the urine samples of patients with essential hypertension (EH) were studied by comparing with healthy people to explore the changes of urine metabolites between hypertensive patients and healthy people in order to find potential biomarkers and metabolic pathways...
August 6, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30086264/variations-of-sclerostin-with-other-bone-biomarkers-over-a-one-year-period-in-hemodialysis-patients
#16
LETTER
François Paquot, Pierre Delanaye, Xavier Warling, Martial Moonen, Nicole Smelten, François Jouret, Jean-Marie Krzesinski, Nicolas Maillard, Hans Pottel, Pieter Evenepoel, Etienne Cavalier
No abstract text is available yet for this article.
August 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30086263/cytokines-in-aortic-dissection
#17
REVIEW
Tao Zeng, Lei Shi, Qingwei Ji, Ying Shi, Ying Huang, Yu Liu, Jianting Gan, Jun Yuan, Zhengde Lu, Yan Xue, Haiying Hu, Ling Liu, Yingzhong Lin
Aortic dissection (AD) is one of the most dangerous forms of vascular disease, characterized by endometrial rupture and intramural hematoma formation. Generally, the pathological process is complicated and closely related to the infiltration of inflammatory cells into the aortic wall and apoptosis of vascular smooth muscle cells. Currently, multiple cytokines, including interleukins, interferon, the tumor necrosis factor superfamily, colony stimulating factor, chemotactic factor, growth factor and so on, have all been demonstrated to play a critical role in AD...
August 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30077639/serum-cyclophilin-a-concentrations-and-prognosis-of-acute-intracerebral-hemorrhage
#18
Bin Chen, Jia Shen, Guan-Rong Zheng, Shen-Zhong Qiu, Huai-Ming Yin, Wei Mao, Hong-Xiang Wang, Jian-Bo Gao
BACKGROUND: Cyclophilin A is involved in brain injury. We investigated the relationship between serum cyclophilin A concentrations, hemorrhagic severity and clinical outcome in intracerebral hemorrhage (ICH). METHODS: We enrolled 105 ICH patients and 105 healthy individuals. Admission serum cyclophilin A concentrations were detected in ICH patients. Hemorrhagic severity was assessed using the National Institutes of Health Stroke Scale (NIHSS) and hematoma volume...
August 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30076803/prognostic-significance-of-stip1-expression-in-human-cancer-a-meta-analysis
#19
REVIEW
Shupeng Zhang, Jianping Shao, Feng Su
BACKGROUND: The prognostic significance of stress-induced protein 1 (STIP1) expression in human cancer has been explored in several studies, however, consensus has not been reached. This meta-analysis aimed to summarize the prognostic value of STIP1 expression in cancer. METHODS: Four common databases were searched to seek relevant studies. The meta-analysis was performed to explore the prognostic value of STIP1 expression in overall survival (OS) and clinicopathological parameters in cancer...
August 1, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30076802/relationship-between-serum-bilirubin-levels-s-and-the-progression-of-renal-function-in-patients-with-chronic-kidney-disease-and-hyperuricemia
#20
Mengyuan Li, Xinhua Li, Yan Liu, Xinying Liu, Yaxiang Song, Jian Zhao, Chandra Mohan, Tianfu Wu, Ai Peng, Ling Qin
It is known that inflammation and oxidative stress have strong influences on chronic kidney disease (CKD). As an antioxidant, bilirubin is currently under extensive scrutiny. However, there are disagreements with regard to the oxidative and antioxidative roles of serum uric acid (SUA). This study aimed to investigate the relationship between serum bilirubin and the progression of renal function in CKD patients with hyperuricemia (HUA). This retrospective longitudinal study included 427 CKD patients. The endpoint was renal replacement therapy or death...
August 1, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
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