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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://www.readbyqxmd.com/read/28427806/decreased-synovial-fluid-ghrelin-levels-are-linked-with-disease-severity-in-primary-knee-osteoarthritis-patients-and-are-increased-following-laser-therapy
#1
Yu-Cong Zou, Hong-Yu Deng, Zheng Mao, Chang Zhao, Ju Huang, Gang Liu
BACKGROUND: Ghrelin has been proved to inhibit inflammation and promote cartilage growth. So far, its role in patients with primary osteoarthritis has not been investigated. OBJECTIVE: The current study was performed to explore the serum and synovial ghrelin levels as well as the relationship between ghrelin levels and disease severity in primary knee OA patients. METHODS: 52 primary knee OA patients were recruited in the study. 52 sex and age-matched patients visiting our hospital for regular body check were selected as controls...
April 18, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28427807/targeted-next-generation-sequencing-in-patients-with-myotonia-congenita
#2
Valentina Ferradini, Marco Cassone, Sara Nuovo, Ilaria Bagni, Rosaria D'Apice, Annalisa Botta, Giuseppe Novelli, Federica Sangiuolo
INTRODUCTION: Myotonia Congenita (MC) is a nondystrophic skeletal muscle disease characterized by muscle stiffness, weakness, delayed skeletal relaxation and hypertrophic muscle. The disease can be inherited as dominant or recessive. More than 130 mutations in CLCN1 gene have been identified. MATERIALS AND METHODS: We analyzed the entire coding region and exon-intron boundaries of the CLCN1 gene in 40 MC patients. Samples already Sanger-sequenced were successively evaluated by Next Generation Sequencing (NGS), on Ion Torrent PGM...
April 17, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28414058/assessment-of-bone-turnover-markers-to-predict-mineral-and-bone-disorder-in-men-with-pre-dialysis-non-diabetic-chronic-kidney-disease
#3
Joseph Jessy Davina, M Priyadarssini, Medha Rajappa, Sreejith Parameswaran, Jayaprakash Sahoo, P S Mohan Raj, G Revathy, C Palanivel, Marie Gilbert Marella
BACKGROUND: Chronic kidney disease (CKD) is commonly associated with disturbances in mineral metabolism and bone disease. Bone biopsy is the gold standard in diagnosing mineral bone disorder. Hence the search for non-invasive assessment of bone health gains importance. We undertook to assess the bone health in men with stage 4 and 5 chronic kidney Disease. METHODS: We recruited 32 male subjects with Stage 4 and 5 chronic kidney disease and 32 age-matched healthy male controls...
April 14, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28412195/neutrophil-lymphocyte-ratio-predicts-hospital-acquired-bacterial-infections-in-decompensated-cirrhosis
#4
Yi-Jing Cai, Jia-Jia Dong, Jin-Zhong Dong, Nai-Bing Yang, Mei Song, Yu-Qun Wang, Yong-Ping Chen, Zhuo Lin, Ke-Qing Shi
BACKGROUND: Bacterial infection is a frequent complication and severe burden in cirrhotic patients. We determined the utility of neutrophil-to-lymphocyte ratio (NLR) to predict the hospital-acquired (HA) bacterial infections episode in patients with decompensated cirrhosis. METHODS: We retrospectively included 2066 consecutive decompensated cirrhotic patients from two separate tertiary hospitals, divided into training (n=1377) and validation (n=689) set. All data were collected on admission and all overt bacterial infections occurring after >48h of hospital stay were registered...
April 13, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28412197/an-uplc-ms-ms-method-for-the-quantification-of-braf-inhibitors-vemurafenib-dabrafenib-and-mek-inhibitors-cobimetinib-trametinib-binimetinib-in-human-plasma-application-to-treated-melanoma-patients
#5
Marine Rousset, Karine Titier, Stephane Bouchet, Caroline Dutriaux, Anne Pham-Ledard, Sorilla Prey, Mireille Canal-Raffin, Mathieu Molimard
Targeted therapies for cancers are fast-growing therapies. For instance kinase inhibitors such as BRAF inhibitors (BRAFi) and MEK inhibitors (MEKi) are increasingly used to treat malignant melanoma. The metabolic profile of these drugs can result in great interindividual variability, justifying therapeutic drug monitoring (TDM). We describe a rapid and specific method for quantification of 2 BRAFi (vemurafenib, dabrafenib) and 3 MEKi (cobimetinib, trametinib and binimetinib). Chromatography was performed on a Waters Acquity-UPLC system with CORTECS C18+ column, under a gradient of 10% acetic acid in water/acetonitrile...
April 12, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28412196/non-enzymatic-glycation-enhances-human-serum-albumin-binding-capacity-to-sodium-fluorescein-at-room-temperature-a-spectroscopic-analysis
#6
Sadaf Fatima, Tamanna Anwar, Nabeel Ahmad, Asimul Islam, Priyankar Sen
BACKGROUND: Sodium fluorescein (SF) is a fluorescent tracer dye used extensively in diagnostic tools in the field of Ophthalmology, particularly in intravenous fluorescein angiography (IVFA). The binding of SF to human serum albumin (HSA) has been predicted by molecular docking and investigated by circular dichroism (CD) and fluorescence spectroscopy with or without glycation at temperatures 296, 301, and 310K. METHODS: The binding parameters were calculated by quenching of emission spectrum of a constant concentration of SF (2μmol/l) at 513nm against increasing concentrations of glycated or unmodified HSA as quencher starting from stoichiometry ratio of 1:1...
April 12, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28410855/methods-of-albumin-estimation-in-clinical-biochemistry-past-present-and-future
#7
REVIEW
Deepak Kumar, Dibyajyoti Banerjee
Estimation of serum and urinary albumin is routinely performed in clinical biochemistry laboratories. In the past, precipitation-based methods were popular for estimation of human serum albumin (HSA). Currently, dye-binding or immunochemical methods are widely practiced. Each of these methods has its limitations. Research endeavors to overcome such limitations are on-going. The current trends in methodological aspects of albumin estimation guiding the field have not been reviewed. Therefore, it is the need of the hour to review several aspects of albumin estimation...
April 11, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28410854/the-correlation-of-serum-bilirubin-levels-with-disease-activity-in-patients-with-rheumatoid-arthritis
#8
You-Fan Peng, Jun-Li Wang, Guo-Gang Pan
BACKGROUND: We investigated the relationship between serum bilirubin and disease activity in patients with rheumatoid arthritis (RA). METHODS: We included a total of 173 consecutive RA patients without steroid treatment and 346 healthy subjects; the disease activity score in 28 joints (DAS28) was used to assess disease activity in patients with RA. RESULTS: Serum bilirubin concentrations were significantly lower in RA patients than in controls...
April 11, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28408198/plasma-8-iso-prostaglandin-f2%C3%AE-a-possible-prognostic-marker-in-aneurysmal-subarachnoid-hemorrhage
#9
De-Sheng Pan, Min Yan, Muhammad Hassan, Ze-Bin Fang, Man-Tao Chen
BACKGROUND: 8-iso-Prostaglandin F2α (8-iso-PGF2α) is a potential biomarker of oxidative stress. This study clarified whether plasma 8-iso-PGF2α concentrations were affected and its underlying relevance to prognosis in aneurysmal subarachnoid hemorrhage (aSAH). METHODS: In this prospective, observational study, a total of 170 controls and 170 aSAH patients were enrolled. Plasma 8-iso-PGF2α concentrations were detected using an ELISA. Severity was assessed by World Federation of Neurological Surgeons (WFNS) scale and modified Fisher grading scale...
April 10, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28396070/novel-pathogenic-acan-variants-in-non-syndromic-short-stature-patients
#10
Xuyun Hu, Baoheng Gui, Jiasun Su, Hongdou Li, Niu Li, Tingting Yu, Qinle Zhang, Yufei Xu, Guoqiang Li, Yulin Chen, Yanrong Qing, Chuan Li, Jingsi Luo, Xin Fan, Yu Ding, Juan Li, Jian Wang, Xiumin Wang, Shaoke Chen, Yiping Shen
BACKGROUND: Pathogenic variants of ACAN have been reported to cause spondyloepiphyseal dysplasia Kimberley type, spondyloepimetaphyseal dysplasia, familial osteochondritis dissecans and idiopathic short stature with normal to advanced bone age. A recent international cohort study significantly expanded the ACAN mutation spectrum, further delineated the heterogeneous clinical characteristics of ACAN mutation patients. The prevalence of ACAN mutation in short stature patients is yet unknown...
April 7, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28392327/new-liquid-chromatography-tandem-mass-spectrometry-method-for-routine-tdm-of-vancomycin-in-patients-with-both-normal-and-impaired-renal-functions-and-comparison-with-results-of-polarization-fluoroimmunoassay-in-light-of-varying-creatinine-concentrations
#11
Hana Brozmanová, Ivana Kacířová, Romana Uřinovská, Pavel Šištík, Milan Grundmann
A new LC-MS/MS method with simple sample extraction and a relatively short period of vancomycin analysis for routine therapeutic drug monitoring was developed and validated. 50μL serum was precipitated using 20μL 33% trichloroacetic acid and 0.5mol/L NH4OH was added to increase pH before analysis. A RP BEH C18, 1.7μm, 2.1×50mm column maintained at 30°C and tobramycin as internal standard were used. Mass detection was performed in positive electrospray mode. The results obtained with LC-MS/MS method were correlated with an FPIA assay (Abbott AxSYM) using mouse monoclonal antibody...
April 7, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28392328/reference-values-for-fasting-serum-resistin-in-healthy-children-and-adolescents
#12
Ulrik Lausten-Thomsen, Michael Christiansen, Paula Louise Hedley, Tenna Ruest Haarmark Nielsen, Cilius Esmann Fonvig, Oluf Pedersen, Torben Hansen, Jens-Christian Holm
BACKGROUND: Resistin is a hormone, mainly produced in macrophages and monocytes, believed to play an important role in the inflammatory response. It has been linked to several chronic diseases such as heart failure, inflammatory bowel disease, and insulin resistance. Pediatric reference levels are needed for the risk stratification and interpretation of individual serum resistin concentrations. METHODS: A total of 1191 healthy, non-obese Danish schoolchildren (727 girls) aged 6-18years (median 11...
April 6, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28385629/a-serum-and-platelet-rich-plasma-serotonin-assay-using-liquid-chromatography-tandem-mass-spectrometry-for-monitoring-of-neuroendocrine-tumor-patients
#13
Catharina M Korse, Johanna C G M Buning-Kager, Theodora C Linders, Annemieke C Heijboer, Daan van den Broek, Margot E T Tesselaar, Olaf van Tellingen, Huub H van Rossum
BACKGROUND: Serotonin is used for the diagnosis and follow-up of neuroendocrine tumors (NET). We describe the analytical and clinical validation of a liquid chromatography tandem mass spectrometry (LC-MS/MS) based serotonin assay for serum and platelet-rich plasma (PRP). METHODS: An LC-MS/MS based method for serum and PRP serotonin was validated by determination of assay imprecision, carry-over, linearity, interference, recovery, sample stability and a matrix/method comparison of serum and PRP serotonin was made with whole blood serotonin...
April 4, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28373054/liquid-biopsy-performance-of-the-paxgene%C3%A2-blood-ccfdna-tubes-for-the-isolation-and-characterization-of-cell-free-plasma-dna-from-tumor-patients
#14
Bernd Schmidt, Dana Reinicke, Iris Reindl, Ines Bork, Bettina Wollschläger, Nina Lambrecht, Michael Fleischhacker
BACKGROUND: In most research laboratories the use of EDTA tubes for the isolation of plasma DNA from tumor patients is standard. Unfortunately these tubes do not allow for an extended storage of samples before processing and prevent EDTA tubes from being shipped at ambient temperature. The aim of our study was to compare the quantity and quality of plasma DNA isolated from EDTA and PAXgene® Blood ccfDNA Tubes in different downstream applications. METHODS: We enrolled 29 patients in our study...
March 31, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28366793/obituary-victor-blaton-1937-2017
#15
EDITORIAL
Joris Delanghe, Michel Langlois
No abstract text is available yet for this article.
March 30, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28366792/serum-macrophage-migration-inhibitory-factor-concentrations-correlate-with-prognosis-of-traumatic-brain-injury
#16
Ding-Bo Yang, Wen-Hua Yu, Xiao-Qiao Dong, Zu-Yong Zhang, Quan Du, Qiang Zhu, Zhi-Hao Che, Hao Wang, Yong-Feng Shen, Li Jiang
BACKGROUND: Macrophage migration inhibitory factor (MIF) is a well-known pro-inflammatory cytokine. Serum MIF concentrations are associated with the severity and prognosis of ischemic stroke. METHODS: In this prospective, observational study, white blood cell (WBC) count and serum concentrations of C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and MIF among 108 severe traumatic brain injury (TBI) patients and 108 controls were measured...
March 30, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28365451/genetic-analysis-and-literature-review-of-chinese-patients-with-familial-renal-glucosuria-identification-of-a-novel-slc5a2-mutation
#17
Xiaojing Wang, Miao Yu, Tong Wang, Huabing Zhang, Fan Ping, Qian Zhang, Jianping Xu, Kai Feng, Xinhua Xiao
BACKGROUND: Familial renal glucosuria (FRG) is an inherited renal tubular disorder characterized by persistent isolated glucosuria with normal blood glucose. SLC5A2 gene mutation was the causative of FRG. METHODS: Molecular genetic analysis of SLC5A2 gene by Sanger sequencing was conducted in two unrelated non-consanguineous Chinese families with isolated glucosuria. Extensive laboratory test and physical examination were performed. In silico algorithms were used to explore the potential effect of novel mutation on SGLT2 function...
March 29, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28365450/a-microrna-expression-profile-for-vascular-invasion-can-predict-overall-survival-in-hepatocellular-carcinoma
#18
Zhuo Lin, Yi-Jing Cai, Rui-Cong Chen, Shi-Hao Xu, Xiao-Dong Wang, Mei Song, Jian-Min Wu, Yu-Qun Wang, Meng-Tao Zhou, Ke-Qing Shi
BACKGROUND: The presence of vascular invasion (VI) in pathology specimens is a well-known unfavorable prognostic factor of hepatocellular carcinoma (HCC) recurrence and overall survival (OS). We investigated the vascular invasion related microRNA (miRNA) expression profiles and potential of prognostic value in HCC. METHODS: MiRNA and mRNA expression data for HCC were accessed from The Cancer Genome Atlas (TCGA). LASSO logistic regression models were used to develop a miRNA-based classifier for predicting VI...
March 29, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28365449/multicenter-evaluation-of-an-enzymatic-method-for-glycated-albumin
#19
Renata Paleari, Graziella Bonetti, Cinzia Callà, Mariarosa Carta, Ferruccio Ceriotti, Nicola Di Gaetano, Marilisa Ferri, Elena Guerra, Gabriella Lavalle, Claudia Lo Cascio, Francesca Gabriela Martino, Martina Montagnana, Marco Moretti, Gabriele Santini, Donata Scribano, Roberto Testa, Anna Vero, Andrea Mosca
BACKGROUND: The use of glycated albumin (GA) has been proposed as an additional glycemic control marker particularly useful in intermediate-term monitoring and in situation when HbA1c test is not reliable. METHODS: We have performed the first multicenter evaluation of the analytical performance of the enzymatic method quantILab Glycated Albumin assay implemented on the most widely used clinical chemistry analyzers (i.e. Abbott Architect C8000, Beckman Coulter AU 480 and 680, Roche Cobas C6000, Siemens ADVIA 2400 and 2400 XPT)...
March 29, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28359780/potential-role-of-retinoids-in-ovarian-physiology-and-pathogenesis-of-polycystic-ovary-syndrome
#20
REVIEW
Yanwen Jiang, Chunjin Li, Lu Chen, Fengge Wang, Xu Zhou
Retinoids (retinol and its derivatives) are required for maintaining vision, immunity, barrier function, reproduction, embryogenesis, cell proliferation and differentiation. Furthermore, retinoid signaling plays a key role in initiating meiosis of germ cells of the mammalian fetal ovary. Recently, studies indicated that precise retinoid level regulation in the ovary provides a molecular control of ovarian development, steroidogenesis and oocyte maturation. Besides, abnormal retinoid signaling may be involved in the pathogenesis of polycystic ovary syndrome (PCOS), one of the most common ovarian endocrinopathies in reproductive-aged women worldwide...
March 27, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
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