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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://www.readbyqxmd.com/read/30517850/measurement-of-urinary-matrix-metalloproteinase-7-for-early-diagnosis-of-acute-kidney-injury-based-on-an-ultrasensitive-immunomagnetic-microparticle-based-time-resolved-fluoroimmunoassay
#1
Junyu Liang, Guanfeng Lin, Jianwei Tian, Jiejing Chen, Rongliang Liang, Zhenhua Chen, Qiaoting Deng, Zhining Dong, Tiancai Liu, Yingsong Wu
The morbidity and mortality associated with acute kidney injury (AKI) remain obstinately high. Early diagnosis is urgently required and should be pursued in at-risk populations. Recently, a newly validated biomarker, matrix metalloproteinase-7 (MMP-7), was reported as a novel indicator for early AKI prediction and a noninvasive surrogate biomarker of kidney function. Monitoring urinary MMP-7 (uMMP-7) levels fills the gaps in early diagnosis of AKI at early onset. However, the lack of available reagents for its rapid detection limits its use...
December 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30517849/a-light-initiated-chemiluminescent-assay-for-rapid-quantitation-of-allergen-specific-igg-4-in-clinical-samples
#2
Junpu Li, Shaoshen Li, Lunhui Huang, Yaqiong Cui, Tiantian She, Ying Bian, Huiqiang Li
BACKGROUND: An increase in allergen-specific IgG4 (sIgG4 ), which serves as a blocking antibody, is associated with acquisition of immune tolerance after immunotherapy. In this study, we developed a rapid, sensitive, and homogeneous immunoassay based on the light-initiated chemiluminescent assay (LICA) technology for quantifying allergen sIgG4 in serum samples. METHODS: Allergen sIgG4 was measured in vitro by incubating the sample with biotinylated allergens and chemiluminescent beads coated with anti-human IgG4 antibody, followed by the addition of streptavidin-coated sensitizer beads...
December 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30508507/chromosomal-microarray-and-whole-exome-sequencing-identify-genetic-causes-of-congenital-hypothyroidism-with-extra-thyroidal-congenital-malformations
#3
Chunyun Fu, Shiyu Luo, Yue Zhang, Xin Fan, Alissa M D'Gama, Xiaofei Zhang, Haiyang Zheng, Jiasun Su, Chuan Li, Jingsi Luo, Pankaj B Agrawal, Qifei Li, Shaoke Chen
BACKGROUND: Congenital hypothyroidism (CH) is the most common neonatal endocrine disorder. Although most patients present with isolated CH, some patients present with CH and extra-thyroidal congenital malformations (ECMs), for which less is known about the underlying genetics. The aim of this study was to investigate the genetic mechanisms in patients with CH and ECMs using chromosomal microarray (CMA) and whole exome sequencing (WES). METHODS: Peripheral venous blood samples were collected from 16 patients with CH and ECMs...
November 30, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30503274/misclassification-of-chronic-hepatitis-b-natural-history-phase-insight-from-new-alt-ast-akp-and-ggt-reference-intervals-in-chinese-children
#4
Yunan Chang, Hu Li, Hong Ren, Hongmei Xu, Peng Hu
BACKGROUND: The age- and sex-specific reference intervals (RIs) for liver chemistry in children are not available in China. Our study aimed to establish age and gender related RIs for ALT, AST, AKP, and GGT in China, and apply the new RI for ALT in children with chronic hepatitis B to use as a biochemical marker for disease progression. METHODS: Data were collected from the Children's Healthcare Center. The measurements of ALT, AST, AKP and GGT were performed on a Hitachi 7600 Chemistry Analyzer...
November 29, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30503273/the-relationship-between-serum-fibrinogen-like-protein-2-concentrations-and-30-day-mortality-of-patients-with-traumatic-brain-injury
#5
Tie-Jiang Chen, Ming-Xia Ji, Zhi-Qiang Tao, Qing-Yang Fu, Kai Xiong, Bin Cheng
BACKGROUND: Fibrinogen-like protein 2 (FGL2) is an inflammatory procoagulant protein. We discerned the impact of serum FGL2 on trauma severity and 30-day mortality in patients with traumatic brain injury (TBI). METHODS: A total of 114 severe TBI patients were subjected to assessment of trauma severity using the Glasgow coma scale (GCS). Measurement of the serum concentrations of FGL2 was done. 114 matched control subjects for their age and sex were included for comparison of serum concentration of FGL2...
November 29, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30502327/quantitative-assessment-of-serum-heat-shock-protein-27-for-the-diagnosis-of-epithelial-ovarian-cancer-using-targeted-proteomics-coupled-with-immunoaffinity-enrichment
#6
Ting Yang, Pengfei Xu, Lize Gu, Zhiyuan Xu, Weihong Ge, Qing Li, Feifei Xu
BACKGROUND: Heat shock protein 27 (HSP27) may take part in the epithelial ovarian cancer (EOC) malignant process because it is elevated in the serum of EOC patients, suggesting that HSP27 may serve as an EOC biomarker to complement the standard serum carbohydrate antigen 125 (CA125) test. Thus, accurate quantification of serum HSP27 would assist the diagnosis of EOC. METHODS: Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted proteomics coupled with an immunoaffinity enrichment assay was developed and validated to monitor HSP27 concentrations in serum...
November 28, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30502326/between-day-versus-within-day-imprecision-using-the-abbott-high-sensitivity-cardiac-troponin-i-assay-at-concentrations-around-5-ng-l
#7
LETTER
Peter A Kavsak, Elspeth Millar, Glenn McLay, Lorna Clark, Allan S Jaffe
No abstract text is available yet for this article.
November 28, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30481500/effects-of-inter-day-and-intra-day-variation-on-salivary-metabolomic-profiles
#8
Norishige Kawanishi, Noriyuki Hoshi, Sugimoto Masahiro, Ayame Enomoto, Sana Ota, Miku Kaneko, Tomoyoshi Soga, Masaru Tomita, Katsuhiko Kimoto
BACKGROUND: Salivary secretion is an important parameter reflecting the health status of an individual and has been used clinically for the diagnosis of various oral diseases, such as xerostomia. Salivary metabolomic profiling is considered an emerging potential tool for the detection of various systemic diseases. To our knowledge, this is the first study to investigate the quantitative relationship between salivary secretion volume and salivary metabolomic profile. METHODS: To evaluate inter- and intra-day variations in salivary secretion, 234 saliva samples were collected three times per day for three days from 13 subjects and analyzed...
November 24, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30472237/autophagy-and-its-role-in-gastric-cancer
#9
REVIEW
Yijing Cao, Yichen Luo, Juan Zou, Jun Ouyang, Zhihong Cai, Xi Zeng, Hui Ling, Tiebing Zeng
Autophagy, which is tightly regulated by a series of autophagy-related genes (ATGs), is a vital intracellular homeostatic process through which defective proteins and organelles are degraded and recycled under starvation, hypoxia or other specific cellular stress conditions. For both normal cells and tumour cells, autophagy not only sustains cell survival but can also promote cell death. Autophagy-related signalling pathways include mTOR-dependent pathways, such as the AMPK/mTOR and PI3K/Akt/mTOR pathways, and non-mTOR dependent pathways, such as the P53 pathway...
November 22, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30471249/applicability-of-liver-stiffness-measurement-based-nomograms-to-the-assessments-of-hepatitis-b-related-significant-fibrosis-and-cirrhosis
#10
Si-Si Ji, Hai-Dan Jiang, Jia-Chun Jiang, Jia Li, Shu-Ting Lin, Bin Chen, Shi-Hao Xu
BACKGROUND: We evaluated liver fibrosis in patients with chronic hepatitis B (CHB) and mildly raised alanine transaminase (ALT) activities between 1-2 times the upper limit of normal (ULN) which was near the threshold for initiating treatment. METHODS: Nomogram-Fibrosis and Nomogram-Cirrhosis were elaborated with variables independently associated with significant fibrosis and cirrhosis determined by multivariate logistic regression. Calibration, receiver operator characteristic (ROC) and decision curves were applied to comparing nomograms with aspartate aminotransferase (AST) to platelet count (PLT) ratio index (APRI), age-AST-PLT-ALT index (FIB-4) and liver stiffness measurement (LSM)...
November 21, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30471248/diagnostic-value-of-cerebro-spinal-fluid-biomarkers-in-dementia-with-lewy-bodies
#11
REVIEW
Olivier Bousiges, Frédéric Blanc
Dementia with Lewy Bodies (DLB) is the second most common form of dementia after Alzheimer's disease (AD), accounting for 15% to 20% of neuropathologically defined cases. Two-thirds of the patients affected are not or misdiagnosed because of the clinical similarity of these two pathologies. In this review, we evaluate the discriminatory power of cerebrospinal fluid (CSF) biomarkers by focusing more specifically on differential diagnosis between DLB and AD. We focus on the AD biological biomarkers used in clinical routine as well as the biomarkers under study and more particularly the alpha-synuclein assay...
November 21, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30468715/noncoding-rna-activated-by-dna-damage-norad-biologic-function-and-mechanisms-in-human-cancers
#12
REVIEW
Zhi Yang, Yongqiang Zhao, Guofu Lin, Xiangyu Zhou, Xiaofeng Jiang, Haiying Zhao
Noncoding RNA activated by DNA damage (NORAD) is a newly identified long non-coding RNA (lncRNA) comprising one exon located on Chr20q11.23. NORAD is unique among lncRNAs because it is highly conserved, abundantly expressed, upregulated upon DNA damage, and maintains chromosomal stability in human cells. NORAD is dysregulated in different types of cancers and has been implicated in several processes correlated with carcinogenesis, such as cell proliferation, invasion, metastasis, and apoptosis. The mechanisms underlying the effects of NORAD are complex and involve multiple factors and signaling pathways...
November 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30468714/changes-of-serum-homocysteine-levels-during-pregnancy-and-the-establishment-of-reference-intervals-in-pregnant-chinese-women
#13
Yuanqing Yang, Hongmin Jiang, Aiguo Tang, Zhongyuan Xiang
BACKGROUD: Reference intervals (RIs) of clinical laboratory indexes are important basis for interpretation of corresponding test results. While elevated homocysteine (HCY) level is a risk factor of some severe gestational diseases, HCY RIs for pregnant women have not been reported so far. The current use of HCY RIs established for general population in pregnant women may challenge clinicians' judgment. This study aims to investigate the changes of serum HCY levels during pregnancy and establish the RIs of serum HCY in healthy pregnant Chinese women to provide valuable data to clinicians and enable the provision of more appropriate therapy...
November 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30452897/dkk1-involvement-and-its-potential-as-a-biomarker-in-pancreatic-ductal-adenocarcinoma
#14
REVIEW
Eseosaserea Igbinigie, Fengbiao Guo, Shi-Wen Jiang, Cullen Kelley, Jinping Li
Dickkopf-1 (Dkk1)'s dysregulation has been implicated in the pathogenesis of a variety of cancers. It is part of the Dkk family of proteins that includes Dkk2, Dkk3 and Dkk4. This family of secreted proteins shares similar conserved cysteine domains and inhibits the Wnt/b-catenin pathway by causing proteasomal B-catenin degradation, inducing apoptosis, and preventing cell proliferation. Pancreatic ductal adenocarcinoma (PDAC) is the 4th leading cause of cancer mortality in the United States due to the late stage of diagnosis and the limited effectiveness of current therapy...
November 16, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30448282/deep-neural-network-for-estimating-low-density-lipoprotein-cholesterol
#15
Taesic Lee, Juwon Kim, Young Uh, Hyunju Lee
BACKGROUND: LDL cholesterol (LDL-C) has been mainly estimated using the Friedewald equation, and other equations have recently been developed to complement the Friedewald equation. The present study aims to employ a deep neural network (DNN) to improve LDL-C estimation. METHODS: We used two independent datasets obtained from the Korean National Health and Nutrition Examination Survey and the Wonju Severance Christian Hospital as training and test datasets, respectively...
November 15, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30448281/soluble-lr11-competes-with-amyloid-%C3%AE-in-binding-to-cerebrospinal-fluid-high-density-lipoprotein
#16
Kouji Yano, Satoshi Hirayama, Naomi Misawa, Ayaka Furuta, Tsuyoshi Ueno, Yumiko Motoi, Utako Seino, Hiroyuki Ebinuma, Takeshi Ikeuchi, Wolfgang J Schneider, Hideaki Bujo, Takashi Miida
BACKGROUND: LR11 is a member of the low-density lipoprotein (LDL) receptor family with high expression in neurons. Some cell surface LR11 is cleaved and secreted into the cerebrospinal fluid (CSF) as soluble LR11 (sLR11). Patients with Alzheimer's disease (AD), particularly apolipoprotein E4 carriers, have high CSF-sLR11 and low CSF-amyloid β (Aβ) concentrations. Therefore, we assessed whether sLR11 is bound to CSF-high-density lipoprotein (HDL) and whether sLR11 competes with Aβ in binding to apoE in CSF-HDL...
November 15, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30445032/a-manifesto-for-the-future-of-laboratory-medicine-professionals
#17
EDITORIAL
Mario Plebani, Michael Laposata, Giuseppe Lippi
Laboratory medicine plays an increasingly essential role in modern healthcare systems, since it is integral to most care pathways and plays an essential role for optimizing patient flow, harmonizing procedures before and after analysis, improving harmonization and containing unnecessary testing. Nevertheless, recent changes in the nature of laboratory services, promoted by innovation and introduction of more complex tests in emerging diagnostic fields, more advanced diagnostics along with other "internal" and "external" drivers, will promote a paradigmatic transformation of current scenarios...
November 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30445031/long-interspersed-nucleotide-element-1-line-1-methylation-in-colorectal-cancer
#18
REVIEW
Mohammad Amin Kerachian, Matin Kerachian
Colorectal cancer (CRC) represents a group of molecularly heterogeneous diseases characterized by genetic and epigenetic alterations. Long interspersed nuclear elements (LINEs) are a form of retrotransposable element found in many eukaryotic genomes. These LINEs, when active, can mobilize in the cell and steadily cause genomic rearrangement. Active LINE reorganization is a source of endogenous mutagenesis and polymorphism in the cell that brings about individual genomic variation. In normal somatic cells, these elements are heavily methylated and thus mostly suppressed, in turn, preventing their potential for bringing about genomic instability...
November 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30445030/development-of-up-converting-phosphor-technology-based-lateral-flow-assay-for-quantitative-detection-of-serum-pivka-ii-inception-of-a-near-patient-pivka-ii-detection-tool
#19
Yi Huang, Songgao Zhang, Qingzhu Zheng, Yadong Li, Lili Yu, Qingwei Wu, Jiaying Zheng, Yanan Wu, Funan Qiu, Qi Gao, Jiancheng Zhang
BACKGROUND: Development of a test card based on up-conversion phosphor technology-based immune lateral flow (UPT-LF) assay as a near-patient detection tool for serum Prothrombin induced by vitamin K absence or antagonist II (PIVKA-II). METHODS: Up-converted phosphor nanoparticles (UCPs) were used to bind to PIVKA-II monoclonal antibodies as labeled probes to develop the test card for detecting serum PIVKA-II. The UPT-LF test card was evaluated by the limit of detection, linearity, stability, recovery rate, precision and interference...
November 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30445029/comparison-of-the-new-and-traditional-ckd-epi-gfr-estimation-equations-with-urinary-inulin-clearance-a-study-of-equation-performance
#20
Christine A White, Céline M Allen, Ayub Akbari, Christine P Collier, David C Holland, Andrew G Day, Greg A Knoll
BACKGROUND: Diagnosis, prognostication and treatment in chronic kidney disease is often informed by an estimate of the glomerular filtration rate (GFR). Commonly used GFR estimation (eGFR) equations are based on serum creatinine (Cr) concentrations and display suboptimal precision and accuracy. Newer equations incorporating additional endogenous markers such as β-Trace Protein (BTP), β2 -Microglobulin (B2M) and cystatin C (cysC) have been developed but require validation. METHODS: This prospective cohort study evaluated the performance of 6 eGFR equations developed by the chronic kidney disease - epidemiology collaboration group (CKD-EPI) against urinary inulin clearance GFR in patients recruited from outpatient nephrology clinics...
November 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
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