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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://www.readbyqxmd.com/read/30326217/performance-of-novel-infection-phase-dependent-antigens-in-syphilis-serodiagnosis-and-treatment-efficacy-determination
#1
Wen Liu, Meixia Deng, Xiaohong Zhang, Weiguo Yin, Tie Zhao, Tiebing Zeng, Shuangquan Liu, Yongjian Xiao, Li Zhang, Xi Luo, Feijun Zhao
OBJECTIVE: The objective of this study was to screen new antigens for syphilis serodiagnosis. METHODS: First, we determined whether the Treponema pallidum proteins Tp0971, Tp0768 and Tp0462 were infection phase-dependent antigens by observing serum reactivity differences in New Zealand rabbits infected with activated or inactivated T. pallidum. A non-infection phase-dependent antigen, the Tp92 membrane protein, was used as the negative control. Next, Tp0971-, Tp0768- and Tp0462-based ELISA was performed on 2138 human serum samples and compared with the T...
October 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30326216/selenium-levels-in-whole-blood-the-borderline-low-analysis
#2
EDITORIAL
S Krishnakumar, Sandhya Iyer, Prachi Sinkar, Caesar Sengupta
OBJECTIVES: Selenium is a very important micronutrient. Food is the major source for selenium uptake and the average blood levels is seen to be highly affected by dietary habits as selenium levels in food naturally depend on the kind of soil they are grown upon and in processed foods its levels depend on fortification. Deficiency of this vital nutriment needs to be assessed to understand deficiency prevalence. DESIGN AND METHODS: A total number of 529,461 subjects were tested for their blood selenium levels...
October 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30321524/utility-of-estimated-glomerular-filtration-rate-using-cystatin-c-and-its-interpretation-in-patients-with-rheumatoid-arthritis-under-glucocorticoid-therapy
#3
Yukiko Nozawa, Hiroe Sato, Ayako Wakamatsu, Daisuke Kobayashi, Takeshi Nakatsue, Yoko Wada, Takeshi Kuroda, Junichiro James Kazama, Yoshiki Suzuki, Masaaki Nakano, Ichiei Narita
BACKGROUND: Patients with rheumatoid arthritis (RA) often have reduced muscle mass. Estimated glomerular filtration ratio using the serum cystatin C concentration (eGFRcys) is more accurate than eGFR using the serum creatinine (eGFRcreat) because cystatin C is not influenced by muscle mass, but glucocorticoid therapy may affect serum cystatin C concentration. METHODS: Fifty patients with RA were included in this study. Renal inulin clearance (Cin) was measured and compared with eGFRcreat, eGFRcys, or the mean of eGFRcreat and eGFRcys (eGFRavg)...
October 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30321523/association-between-pahs-biomarkers-and-kidney-injury-biomarkers-among-kitchen-workers-with-microalbuminuria-a-cross-sectional-pilot-study
#4
Amarnath Singh, Ritul Kamal, Ratnakar Tiwari, Vivek Kumar Gaur, Vipin Bihari, G N V Satyanarayana, Devendra Kumar Patel, Parappurath Abdul Azeez, Vikas Srivastava, Chandrasekharan Nair Kesavachandran
BACKGROUND: To study the association between kidney injury biomarkers and urinary OH-PAH metabolites in kitchen workers, with microalbuminuria. METHODS: A cross-sectional pilot study was conducted among 120 male kitchen workers in a mega kitchen located at Coimbatore, India. Personal and, sub-clinical details of study subjects were collected using a questionnaire. Albumin, creatinine, and albumin-creatinine ratio (ACR) were measured using urine dipstick test for the determination of microalbuminuria...
October 12, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30317022/whole-exome-sequencing-for-the-genetic-diagnosis-of-congenital-red-blood-cell-membrane-disorders-in-taiwan
#5
Pei-Chin Lin, Shyh-Shin Chiou, Chien-Yu Lin, Shu-Chen Wang, Hsi-Yuan Huang, Ya-Sian Chang, Yu-Hsin Tseng, Tzu-Min Kan, Yu-Mei Liao, Shi-Pien Tsai, Ching-Tien Peng, Jan-Gowth Chang
PURPOSE: Congenital hemolytic anemia caused by red blood cell (RBC) membrane defects is a heterogeneous group of disorders. The present study aimed to search the causative gene mutations in patients with RBC membrane disorders in Taiwan. MATERIALS AND METHODS: Next-generation sequencing approach using whole-exome sequencing (WES) was performed. Sanger sequencing was performed for confirmation of variants detected in WES in patients and their family members. RESULTS: Five causative variants, including two ANK1, two SPTA and one SPTB variants, were detected in four patients...
October 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30317021/a-case-of-monoclonal-gammopathy-of-undetermined-significance-with-abnormal-low-levels-of-plasma-glycated-albumin-by-m-protein
#6
Noriko Kuramoto, Tatsuya Wakahara, Yuichi Tamagawa, Takahiro Uchino, Masahiro Koseto, Manabu Kawakami, Michio Otsuki, Yoshimasa Aoki, Midori Ishibashi, Soji Kasayama, Satoru Sumitani, Masafumi Koga
BACKGROUND: It is known that an immunoglobulin abnormality affects various clinical laboratory measurements and leads to abnormal values. We experienced a case of monoclonal gammopathy of undetermined significance (MGUS) showing a falsely low plasma glycated albumin (GA) level. CASE REPORT: The patient was a 75-y-old male who visited our hospital for thrombocytosis identified during a medical checkup. Based on further examinations, he was diagnosed with MGUS (IgM-κ type)...
October 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30317020/effect-of-hemolysis-icterus-and-lipemia-on-three-acetaminophen-assays-potential-medical-consequences-of-false-positive-results
#7
Y Victoria Zhang, Shu-Ling Fan, Mark D Kellogg
BACKGROUND: We evaluated the effect of hemolysis, icterus and lipemia on 3 acetaminophen assays: namely the Syva® EMIT®, the Microgenics DRI® assay, and the Roche assay on a Roche Cobas® c501 or an Integra 800 analyzer. METHODS: Discarded acetaminophen - free serum samples (blank pool) and patient serum with acetaminophen overdose were used to prepare samples. Three levels of acetaminophen (5, 10, and 30 μg/ml) were evaluated for interference: hemolysis (H index range: 0-1000), icterus (I index range: 0-40), and lipemia (L index range: 0-1000)...
October 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30315756/pseudohyperphosphatemia-in-a-patient-with-incidentially-identified-progression-of-smoldering-myeloma
#8
Elizabeth R Francis, Fengming Chen, Michal Kidacki, Ronald Miller, Mo'tasem Alkhasoneh, Giampaolo Talamo, Monica Straub, Kristin Klinefelter, Namratha Kodali, Yusheng Zhu
BACKGROUND: Pseudohyperphosphatemia is a rare laboratory finding in MM, especially in patients with smoldering myeloma (SMM) progressing to symptomatic multiple myeloma (MM). Laboratorians and clinicians should be aware of this phenomenon and take necessary actions to avoid misdiagnosis. METHODS: Specimens from a monoclonal IgG kappa SMM patient with extremely high serum phosphorus concentrations measured by the Roche phosphomolybdate assay were re-evaluated using serial dilutions and the ORTHO VITROS assay free from monoclonal gammaglobulin interference...
October 10, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30312599/performance-evaluation-of-hba1c-measurement-systems-with-sigma-metric-for-1066-laboratories-in-china
#9
Yuzhu Huang, Tianjiao Zhang, Haijian Zhao, Wei Wang, Chuanbao Zhang, Falin He, Kun Zhong, Shuai Yuan, Yuxuan Du, Zhiguo Wang
BACKGROUND: This study aimed to evaluate the performance of HbA1c measurement systems quantitatively with sigma metric and promote the quality improvement. METHODS: 1066 laboratories enrolled external quality assessment (EQA) and reported their internal quality control (IQC) data for HbA1c in 2017 were included in this study. The bias and coefficient of variation were collected from EQA and IQC program designed by the National Center for Clinical Laboratory. The σ values was used to assess the performance of HbA1c, and combine Westgard Sigma Rules™ to select proper quality control rules in every laboratory...
October 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30308187/a-rare-stap1-mutation-incompletely-associated-with-familial-hypercholesterolemia
#10
Francisco Blanco-Vaca, Jesús M Martín-Campos, Antonio Pérez, Pablo Fuentes-Prior
Autosomal dominant hypercholesterolemia, being referred to as familial hypercholesterolemia (FH), is mainly due to defective LDL receptor (LDLR) function, but is also associated with variants in genes encoding APOB (LDLR ligand) and PCSK9, the catabolic regulator of LDLR. The signal-transducing adaptor family member 1 (STAP1) gene has been recently linked to FH. We describe the case of a 56-year-old male patient found to have hypercholesterolemia at age 34, but who did not continue follow-up nor received treatment with lipid-lowering drugs...
October 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30296440/evaluation-of-the-siemens-advia-centaur-high-sensitivity-cardiac-troponin-i-assay-in-serum
#11
Peter A Kavsak, Andrew Worster, Stephen A Hill, Allan S Jaffe
BACKGROUND: International recommendations on high-sensitivity cardiac troponin (hs-cTn) testing recommend that laboratories select only one sample type for testing. We evaluated the Siemens ADVIA Centaur hs-cTnI assay in serum and thereby provide information on imprecision, long-term storage stability, freeze-thaw stability, method comparison to other hs-cTnI assays, and clinical performance. METHODS: Patients with chest pain onset <6 h who already had Roche hs-cTnT, Beckman hs-cTnI and Abbott hs-cTnI results recorded and had non-thawed and frozen serum aliquots formed the study population (n = 134 patients with 305 serum aliquots obtained at either 0, 3 or 6 h stored below -70 °C since 2003) for measurement with the Siemens hs-cTnI assay in 2018...
October 6, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30296444/plasma-metabolomic-profiling-distinguishes-right-sided-from-left-sided-colon-cancer
#12
Kui Deng, Peng Han, Wei Song, Zhuozhong Wang, Fan Zhang, Hongyu Xie, Weiwei Zhao, Huan Xu, Yuqing Cai, Zhiwei Rong, Xiwen Yu, Bin-Bin Cui, Kang Li
BACKGROUND: Many studies have demonstrated that right-sided colon cancer (RCC) has a higher mortality rate and worse prognosis than left-sided colon cancer (LCC). However, the underlying biological mechanism that can account for these differences is unclear. METHODS: In this study, plasma metabolic profiles in 147 LCC patients and 105 RCC patients were systematically analyzed by the ultra high performance liquid chromatography quadruple time-of-flight mass spectrometry (UHPLC-QTOF/MS) platform in conjunction with univariate and multivariate statistical analysis...
October 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30296443/apelin-apj-system-a-novel-promising-target-for-anti-aging-intervention
#13
REVIEW
Qionglin Zhou, Linxi Chen, Mingzhu Tang, Yu Guo, Lanfang Li
Apelin, an endogenous ligand for the G protein-coupled receptor APJ, is widely expressed in various organs. Recent research has indicated that the Apelin/APJ system plays an important role in aging. Apelin and APJ receptor expression are down-regulated with increasing age. In murine models, Apelin and APJ knockouts exhibit accelerated senescence whereas Apelin-restoration results in enhanced vigor and rejuvenated behavioral and circadian phenotypes. Furthermore, aged Apelin knockout mice develop progressive impairment of cardiac contractility associated with systolic dysfunction...
October 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30296442/comprehensive-assessment-of-biotin-interference-in-immunoassays
#14
Jieli Li, Elizabeth A Wagar, Qing H Meng
BACKGROUND: Biotinylated antibodies and analogues are currently used in many immunoassays while biotin is widely used as a dietary supplement. Thus, biotin interference is an emerging issue for clinical laboratories. METHODS: Various concentrations of biotin solutions were prepared using pooled patient serum samples. All analytes were measured by sandwich or competitive immunoassay on the Roche Cobas 8000 e602 platform. RESULTS: Some of the sandwich immunoassay results were falsely decreased to different extents by different biotin levels, while some of the competitive immunoassay results were falsely increased...
October 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30296441/characterization-of-plac%C3%A2-tests-in-the-quantization-of-lipoprotein-associated-phospholipase-a-2-for-assessment-of-cardiovascular-diseases
#15
Shaoqiu Zhuo, Chong Yuan
BACKGROUND: PLAC® mass test (diaDexus, Inc.) does not detect all Lp-PLA2 proteins in the circulation. The total circulating Lp-PLA2 mass can be quantized by using the CHAPS modified PLAC® mass test. To compare the difference of the PLAC® mass, CHAPS modified PLAC® mass and PLAC® activity tests in risk assessment of CVD, the 3 Lp-PLA2 quantization methods were characterized using a collection of serum and plasma from CVD patents and matched non-symptomatic controls. Improvement on risk assessment for ischemic stroke by Lp-PLA2 and lipids were also investigated...
October 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30292629/establishment-of-a-protein-biochip-to-detect-serum-igg-antibodies-against-il-2-and-soluble-cd25-in-hemophagocytic-lymphohistiocytosis
#16
Qian Liu, Sheng-Sheng Liu, Song-Guo Li, Yi Gao, Lei Ye, Gabrielle Olivia Ramsay Johnson, Zi-Jian Song, Wei-Dong Du
BACKGROUND: Interleukin-2 (IL-2) and soluble CD25 (sCD25) are among the most important cytokines and diagnostic biomarkers in hemophagocytic lymphohistiocytosis (HLH). Detecting serum level of IL-2 and sCD25 is valuable for making clinical diagnosis and treatment decision in HLH. METHODS: Since tests showing serum IgG antibody against IL-2 or sCD25 have never been carried out, a new protein biochip, which was modified with cysteine and activated sophorolipid (Cys-SL), was developed...
October 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30292632/is-capillary-zone-electrophoresis-a-suitable-method-for-estimating-serum-albumin-a-comparison-of-four-methods
#17
Padelli Maël, Leven Cyril, Le Reun Eymeric, Labouret Théo, Galakhoff Nicolas, Labarre Marion, Rouillé Amélie, Kerspern Hélène, Lapègue Marion, Juliette Chauvet, Plée-Gautier Emmanuelle, Carré Jean-Luc
BACKGROUND: The capillary zone electrophoresis method of albumin measurement is frequently used for oncologic and haematologic patients but few data exist about the agreement between the albumin measurements performed by capillary zone electrophoresis and other methods. The aim of this study was to analyse the agreement between human serum albumin measurements by capillary zone electrophoresis and by the nephelometry, bromocresol purple and turbidimetry methods. METHOD: We prospectively measured 100 freshly collected non-frozen patient serum samples, by using four different methods: the capillary zone electrophoresis method performed with a CAPILLARYS 2 instrument, the bromocresol purple dye method performed with an Advia XPT analyser, the nephelometric method performed with a BN ProSpec analyser and the turbidimetric method with reagents from DiAgam and performed with the Advia XPT analyser...
October 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30292631/quality-specifications-and-their-daily-application-to-evaluate-the-accuracy-of-reference-measurements-for-serum-concentrations-of-25-hydroxyvitamin-d-3-and-25-hydroxyvitamin-d-2
#18
Ekaterina M Mineva, Maya R Sternberg, Christine M Pfeiffer, Shahzad S Momin, Khin L Maw, Rosemary L Schleicher
BACKGROUND: Reference measurement procedures (RMP) have rigorous accuracy specifications. For total 25-hydroxyvitamin D, 25(OH)D, bias ≤1.7% and CV ≤5% are recommended. These quality specifications are impractical for minor analytes, such as 25(OH)D2 . Furthermore, documentation on RMP quality performance specifications for the individual 25(OH)D metabolites and their daily application are missing. METHODS: To assess accuracy, we used zeta-scores. Daily, 5-10 specimens (duplicate) and 3 reference materials (singleton or duplicate) were measured for 25(OH)D3 and 25(OH)D2 using JCTLM-accepted LC-MS/MS RMPs...
October 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30292630/targeted-exome-sequencing-identified-a-novel-mutation-hotspot-and-a-deletion-in-chinese-primary-hypertrophic-osteoarthropathy-patients
#19
Yulin Chen, Guoqiang Li, Yufei Xu, Tingting Yu, Yi Zhang, Niu Li, Ru-En Yao, Yunfang Zhou, Xiumin Wang, Yiping Shen, Lei Yin, Jian Wang
BACKGROUND: Primary hypertrophic osteoarthropathy (PHO) is a genetically and clinically heterogeneous systematic disorder caused by mutations in genes HPGD and SLCO2A1. The purpose of the present study is to provide useful information for the early and precise diagnosis of PHO and identify causative mutations in Chinese PHO children. METHODS AND RESULTS: The clinical manifestations, radiographic features of seven Chinese pediatric patients were systematically analyzed...
October 4, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/30291896/c-src-tyrosine-kinase-gene-rs1378942-polymorphism-and-hypertension-in-asians-review-and-meta-analysis
#20
REVIEW
Xinling Qian, Yuqian Li, Xiaotian Liu, Runqi Tu, Zhongyan Tian, Haiqing Zhang, Xia Zhang, Wen Zhou, Jingjing Jiang, Yanhua Wang, Ronghai Bie, Chongjian Wang
BACKGROUND: The risk allele frequency of rs1378942 of c-src tyrosine kinase (CSK) gene is much higher in Asians (~ 0.80) than that in Europeans (~ 0.35), and the association between rs1378942 and blood pressure is controversial in Asians. Thus, the review and meta-analysis was performed to explore the effects of rs1378942 polymorphism on systolic blood pressure (SBP), diastolic blood pressure (DBP) levels and hypertension in Asians. METHODS: A literature search was conducted in electronic databases up to September 1, 2018...
October 3, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
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