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Biochimie

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https://www.readbyqxmd.com/read/28196677/molecular-modeling-docking-and-dynamics-simulations-of-the-dioclea-lasiophylla-mart-ex-benth-seed-lectin-an-edematogenic-and-hypernociceptive-protein
#1
Vanir Reis Pinto-Junior, Vinicius Jose Silva Osterne, Mayara Queiroz Santiago, Claudia Figueiredo Lossio, Celso Shiniti Nagano, Cintia Renata Costa Rocha, Jessica Catarine Frutuoso Nascimento, Francisco Lucas Faustino Nascimento, Ivanice Bezerra Silva, Antonia Simoni Oliveira, Jorge Luis Almeida Correia, Rodrigo Bainy Leal, Ana Maria Sampaio Assreuy, Benildo Sousa Cavada, Kyria Santiago Nascimento
Lectins are proteins, or glycoproteins, capable of reversibly binding to specific mono- or oligosaccharides via a noncatalytic domain. The Diocleinae subtribe presents lectins with high structural similarity, but different effects based on biological activity assays. This variability results from small structural differences. Therefore, in this context, the present study aimed to perform a structural analysis of the lectin from Dioclea lasiophylla Mart. ex Benth seeds (DlyL) and evaluate its inflammatory effect...
February 11, 2017: Biochimie
https://www.readbyqxmd.com/read/28196676/dihydroorotate-dehydrogenase-dhodh-inhibitors-affect-atp-depletion-endogenous-ros-and-mediate-s-phase-arrest-in-breast-cancer-cells
#2
A K Mohamad Fairus, B Choudhary, S Hosahalli, N Kavitha, O Shatrah
Dihydroorotate dehydrogenase (DHODH) is the key enzyme in de novo biosynthesis of pyrimidine in both prokaryotes and eukaryotes. The de novo pathway of pyrimidine biosynthesis is essential in cancer cells proliferation. Leflunomide is an approved DHODH inhibitor that has been widely used for the treatment of arthritis. Similarly, brequinar sodium is another DHODH inhibitor that showed anti-tumour effect in MC38 colon carcinoma cells when used in combination with fluorouracil. Despite the potential role of DHODH inhibitors in cancer therapy, their mechanisms of action remain obscure and await further elucidation...
February 11, 2017: Biochimie
https://www.readbyqxmd.com/read/28192157/apaf-1-regulation-and-function-in-cell-death
#3
REVIEW
Raheleh Shakeri, Asma Kheirollahi, Jamshid Davoodi
Apoptosis, a form of programmed cell death, is responsible for eliminating damaged or unnecessary cells in multicellular organisms. Various types of intracellular stress trigger apoptosis by induction of cytochrome c release from mitochondria into the cytosol. Apoptotic protease activating factor-1 (Apaf-1) is a key molecule in the intrinsic or mitochondrial pathway of apoptosis, which oligomerizes in response to cytochrome c release and forms a large complex known as apoptosome. Procaspase-9, an initiator caspase in the mitochondrial pathway, is recruited and activated by the apoptosome leading to downstream caspase-3 processing...
February 9, 2017: Biochimie
https://www.readbyqxmd.com/read/28153695/lipid-load-in-peripheral-blood-mononuclear-cells-impact-of-food-consumption-dietary-macronutrients-extracellular-lipid-availability-and-demographic-factors
#4
Fatima Ameer, Rimsha Munir, Hina Usman, Rida Rashid, Muhammad Shahjahan, Shahida Hasnain, Nousheen Zaidi
Lipid-load in peripheral blood mononuclear cells (PBMCs) has recently gained attention of the researchers working on nutritional regulation of metabolic health. Previous works have indicated that the metabolic circuitries in the circulating PBMCs are influenced by dietary-intake and macronutrient composition of diet. In the present work, we analyzed the impact of diet and dietary macronutrients on PBMCs' lipid-load. The overall analyses revealed that dietary carbohydrates and fats combinatorially induce triglyceride accumulation in PBMCs...
January 30, 2017: Biochimie
https://www.readbyqxmd.com/read/28153694/production-in-pichia-pastoris-antifungal-activity-and-crystal-structure-of-a-class-i-chitinase-from-cowpea-vigna-unguiculata-insights-into-sugar-binding-mode-and-hydrolytic-action
#5
Patrícia G Castro Landim, Tuana O Correia, Fredy D A Silva, Denise R Nepomuceno, Helen P S Costa, Humberto M Pereira, Marina D P Lobo, Frederico B M B Moreno, José Brandão-Neto, Suelen C Medeiros, Ilka M Vasconcelos, José T A Oliveira, Bruno L Sousa, Ito L Barroso-Neto, Valder N Freire, Cristina P S Carvalho, Ana C O Monteiro-Moreira, Thalles B Grangeiro
A cowpea class I chitinase (VuChiI) was expressed in the methylotrophic yeast P. pastoris. The recombinant protein was secreted into the culture medium and purified by affinity chromatography on a chitin matrix. The purified chitinase migrated on SDS-polyacrylamide gel electrophoresis as two closely-related bands with apparent molecular masses of 34 and 37 kDa. The identity of these bands as VuChiI was demonstrated by mass spectrometry analysis of tryptic peptides and N-terminal amino acid sequencing. The recombinant chitinase was able to hydrolyze colloidal chitin but did not exhibit enzymatic activity toward synthetic substrates...
January 30, 2017: Biochimie
https://www.readbyqxmd.com/read/28143796/clr4-specificity-and-catalytic-activity-beyond-h3k9-methylation
#6
Denis Kusevic, Srikanth Kudithipudi, Nahid Iglesias, Danesh Moazed, Albert Jeltsch
In fission yeast, the catalytic activity of the protein lysine methyltransferase (PKMT) Clr4, the sole homolog of the mammalian SUV39H1 and SUV39H2 enzymes, majorly contributes to the formation of heterochromatin. The enzyme introduces histone 3 lysine 9 (H3K9) di- and tri-methylation, a central heterochromatic histone modification, and later it was also found to methylate the Mlo3 protein, which has a role in heterochromatin formation as well. Herein, we have investigated the substrate specificity of Clr4 using custom made mutational scanning peptide arrays...
January 28, 2017: Biochimie
https://www.readbyqxmd.com/read/28137394/corrigendum-to-fragile-x-syndrome-are-signaling-lipids-the-missing-culprits-biochimie-130c-2016-188-194
#7
Ricardos Tabet, Nicolas Vitale, Hervé Moine
No abstract text is available yet for this article.
January 27, 2017: Biochimie
https://www.readbyqxmd.com/read/28115185/plasma-kallikrein-enhances-platelet-aggregation-response-by-subthreshold-doses-of-adp
#8
Tatiana F Ottaiano, Sheila S Andrade, Cleide de Oliveira, Mariana C C Silva, Marcus V Buri, Maria A Juliano, Manoel J B C Girão, Misako U Sampaio, Alvin H Schmaier, Alexander Wlodawer, Francisco H A Maffei, Maria L V Oliva
Human plasma kallikrein (huPK) potentiates platelet responses to subthreshold doses of ADP, although huPK itself, does not induce platelet aggregation. In the present investigation, we observe that huPK pretreatment of platelets potentiates ADP-induced platelet activation by prior proteolysis of the G-protein-coupled receptor PAR-1. The potentiation of ADP-induced platelet activation by huPK is mediated by the integrin αIIbβ3 through interactions with the KGD/KGE sequence motif in huPK. Integrin αIIbβ3 is a cofactor for huPK binding to platelets to support PAR-1 hydrolysis that contributes to activation of the ADP signaling pathway...
January 20, 2017: Biochimie
https://www.readbyqxmd.com/read/28111291/cd36-gene-polymorphism-is-associated-with-alzheimer-s-disease
#9
Omar Šerý, Jana Janoutová, Laura Ewerlingová, Alice Hálová, Jan Lochman, Vladimír Janout, Naim A Khan, Vladimir J Balcar
CD36 gene encodes a membrane glycoprotein (type B scavenger receptor) present on the surface of many types of cells and having multiple cellular functions ranging from angiogenesis to gustatory perception of fatty acids. Using a case control genetic association approach we have analyzed selected single nucleotide polymorphisms (SNP's) in a total of 859 patients with Alzheimer's disease (AD) and controls and have identified the allele T in rs3211893 polymorphism of CD36 gene as significantly increasing the risk of AD...
January 19, 2017: Biochimie
https://www.readbyqxmd.com/read/28111290/suppression-of-neu1-sialidase-delays-the-absorption-of-yolk-sac-in-medaka-oryzias-latipes-accompanied-with-the-accumulation-of-%C3%AE-2-3-sialo-glycoproteins
#10
Sena Ryuzono, Ryo Takase, Yuko Kamada, Takanori Ikenaga, Petros Kingstone Chigwechokha, Masaharu Komatsu, Kazuhiro Shiozaki
Sialidase catalyzes the removal of sialic acids from glycoconjugates. Recently, medaka sialidase Neu1 has been cloned and its enzymatic properties were investigated. Although enzymatic properties of this sialidase, such as optimal pH and substrate specificity, exhibits high similarity with human NEU1, Neu1 physiological functions in fish are still unclear. Here, to understand Neu1 significance in medaka embryogenesis, sialidase translation knockdown was carried out with one-cell stage fertilized egg using morpholino oligo injection...
January 19, 2017: Biochimie
https://www.readbyqxmd.com/read/28109719/the-expanding-repertoire-of-g4-dna-structures
#11
Anna Varizhuk, Dmitry Ischenko, Vladimir Tsvetkov, Roman Novikov, Nikolay Kulemin, Dmitry Kaluzhny, Maria Vlasenok, Vladimir Naumov, Igor Smirnov, Galina Pozmogova
The definition of DNA and RNA G-quadruplexes (G4s) has recently been broadened to include structures with certain defects: bulges, G-vacancies or mismatches. Despite the striking progress in computational methods for assessing G4 folding propensity, predicting G4s with defects remains problematic, reflecting the enhanced sequential diversity of these motifs. "Imperfect" G4 motifs, i.e., those containing interrupted or truncated G-runs, are typically omitted from genomic analyses. We report here studies of G4s with defects and compare these structures with classical ("perfect") quadruplexes...
January 18, 2017: Biochimie
https://www.readbyqxmd.com/read/28104508/exposure-to-high-levels-of-fumarate-and-succinate-leads-to-apoptotic-cytotoxicity-and-altered-global-dna-methylation-profiles-in-vitro
#12
Johannes F Wentzel, Angélique Lewies, Abel J Bronkhorst, Etresia van Dyk, Lissinda H du Plessis, Piet J Pretorius
In the Krebs cycle, succinate is oxidized to fumarate by succinate dehydrogenase (SDH), followed by the conversion of fumarate to malate by fumarate hydratase (FH). In cells with defective SDH and FH, the Krebs cycle is congested, respiration impaired and fumarate and succinate accumulates. Several studies have indicated that the accumulation of these substrates are associated with cytotoxicity and oncogenesis. High levels of succinate and fumarate induce hypoxia inducible factor (HIF1A) hydroxylases, leading to the activation of oncogenic HIF pathways...
January 16, 2017: Biochimie
https://www.readbyqxmd.com/read/28104507/insights-into-ligand-binding-to-a-glutathione-s-transferase-from-mango-structure-thermodynamics-and-kinetics
#13
Ignacio Valenzuela-Chavira, Carmen A Contreras-Vergara, Aldo A Arvizu-Flores, Hugo Serrano-Posada, Alonso A Lopez-Zavala, Karina D García-Orozco, Javier Hernandez-Paredes, Enrique Rudiño-Piñera, Vivian Stojanoff, Rogerio R Sotelo-Mundo, Maria A Islas-Osuna
We studied a mango glutathione S-transferase (GST) (Mangifera indica) bound to glutathione (GSH) and S-hexyl glutathione (GSX). This GST Tau class (MiGSTU) had a molecular mass of 25.5 kDa. MiGSTU Michaelis-Menten kinetic constants were determined for their substrates obtaining a Km, Vmax and kcat for CDNB of 0.792 mM, 80.58 mM min(-1) and 68.49 s(-1) respectively and 0.693 mM, 105.32 mM min(-1) and 89.57 s(-1), for reduced GSH respectively. MiGSTU had a micromolar affinity towards GSH (5.2 μM) or GSX (7...
January 16, 2017: Biochimie
https://www.readbyqxmd.com/read/28087294/bioactive-metabolites-of-docosahexaenoic-acid
#14
REVIEW
Ondrej Kuda
Docosahexaenoic acid (DHA) is an essential fatty acid that is recognized as a beneficial dietary constituent and as a source of the anti-inflammatory specialized proresolving mediators (SPM): resolvins, protectins and maresins. Apart from SPMs, other metabolites of DHA also exert potent biological effects. This article summarizes current knowledge on the metabolic pathways involved in generation of DHA metabolites. Over 70 biologically active metabolites have been described, but are often discussed separately within specific research areas...
January 10, 2017: Biochimie
https://www.readbyqxmd.com/read/28082125/oligomeric-state-of-hypoxanthine-guanine-phosphoribosyltransferase-from-mycobacterium-tuberculosis
#15
Wai Soon Eng, Dianne T Keough, Dana Hockova, Donald J Winzor, Luke W Guddat
Sedimentation equilibrium and size-exclusion chromatography experiments on Mycobacterium tuberculosis hypoxanthine-guanine phosphoribosyltransferase (MtHGPRT) have established the existence of this enzyme as a reversibly associating mixture of dimeric and tetrameric species in 0.1 M Tris-HCl-0.012 M MgCl2, pH 7.4. Displacement of the equilibrium position towards the larger oligomer by phosphate signifies the probable existence of MtHGPRT as a tetramer in the biological environment. These data thus add credibility to the relevance of considering enzyme function in the light of a published tetrameric structure deduced from X-ray crystallography...
January 9, 2017: Biochimie
https://www.readbyqxmd.com/read/28077274/the-role-and-regulation-of-the-peroxisome-proliferator-activated-receptor-alpha-in-human-liver
#16
REVIEW
Sander Kersten, Rinke Stienstra
The peroxisome proliferator-activated receptor α (PPARα) is a ligand-activated transcription factor that is abundantly expressed in liver. PPARα is activated by fatty acids and various other lipid species, as well as by a class of chemicals referred to as peroxisome proliferators. Studies in mice have shown that PPARα serves as the master regulator of hepatic lipid metabolism during fasting. In addition, PPARα suppresses inflammation and the acute phase response. Comparatively little is known about PPARα in human liver...
January 7, 2017: Biochimie
https://www.readbyqxmd.com/read/28065690/medium-throughput-biochemical-compound-screening-identifies-novel-agents-for-pharmacotherapy-of-neurofibromatosis-type-i
#17
Galina Semenova, Dina Stepanova, Sergey M Deyev, Jonathan Chernoff
The variable manifestation of phenotypes that occur in patients with neurofibromatosis type 1 (NF1) includes benign and malignant neurocutaneous tumors for which no adequate treatment exists. Cell-based screening of known bioactive compounds library identified the protein phosphatase 2A (PP2A) inhibitor Cantharidin and the L-type calcium channel blocker Nifedipine as potential candidates for NF1 pharmacotherapy. Validation of screening results using human NF1-associated malignant peripheral nerve sheath tumor (MPNST) cells showed that Cantharidin effectively impeded MPNST cell growth, while Nifedipine treatment significantly decreased local tumor growth in an MPNST xenograft animal model...
January 5, 2017: Biochimie
https://www.readbyqxmd.com/read/28057584/coregulator-mediated-control-of-skeletal-muscle-plasticity-a-mini-review
#18
Svenia Schnyder, Barbara Kupr, Christoph Handschin
Skeletal muscle plasticity is a complex process entailing massive transcriptional programs. These changes are mediated by the action of nuclear receptors and other transcription factors. In addition, coregulator proteins have emerged as important players in this process by linking transcription factors to the RNA polymerase II complex and inducing changes in the chromatic structure. An accumulating body of work highlights the pleiotropic functions of coregulator proteins in the control of tissue-specific and whole body metabolism...
January 2, 2017: Biochimie
https://www.readbyqxmd.com/read/28038935/defense-peptide-repertoire-of-stellaria-media-predicted-by-high-throughput-next-generation-sequencing
#19
Anna A Slavokhotova, Andrey A Shelenkov, Tatyana V Korostyleva, Eugene A Rogozhin, Nataliya V Melnikova, Anna V Kudryavtseva, Tatyana I Odintsova
Being perfectly adapted to diverse environments, chickweed (Stellaria media (L.) Vill), a ubiquitous garden weed, grows widely in Europe and North America. As opposed to the model plants, many weeds, and S. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. In this study, for the first time RNA-seq analysis of healthy and infected with Fusarium oxysporum chickweed seedlings, as well as de novo transcriptome assembly and annotation, are presented...
December 27, 2016: Biochimie
https://www.readbyqxmd.com/read/28034718/control-of-adipogenesis-by-oxylipins-gpcrs-and-ppars
#20
REVIEW
Valentin Barquissau, Rayane A Ghandour, Gérard Ailhaud, Martin Klingenspor, Dominique Langin, Ez-Zoubir Amri, Didier F Pisani
Oxylipins are bioactive metabolites derived from the oxygenation of ω3 and ω6 polyunsaturated fatty acids, triggered essentially by cyclooxygenase and lipoxygenase activities. Oxylipins are involved in the development and function of adipose tissue and their productions are strictly related to diet quality and quantity. Oxylipins signal via cell surface membrane (G Protein-coupled receptors) and nuclear receptors (peroxisome proliferator-activated receptors), two pathways playing a pivotal role in adipocyte biology...
December 27, 2016: Biochimie
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