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Archiwum Medycyny Sa̧dowej i Kryminologii

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https://www.readbyqxmd.com/read/28155989/-first-successful-dna-isolation-and-profiling-from-bone-using-an-approach-that-is-non-destructive-toward-bone-surface
#1
Dominika Pluta, Arleta Lebioda, Anna Jonkisz, Tadeusz Dobosz
Commonly used destructive DNA isolation techniques consist of pulverization which leads to the complete destruction of smaller bones or irreversible damage to larger bones through the cutting of extensive fragments. The procedure is totally unacceptable when handling bones which are valuable for anthropological or religious reasons, as museum exhibits or due to emotional factors. Since the Department's team has already resolved this problem in the case of human teeth (A. Pilecka), efforts have been undertaken to develop a similar non-destructive technique for the isolation of DNA from human bones...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28155988/-hunting-shot-evolution-of-manufacturing-technology
#2
Piotr Bochyński, Maciej Kuliczkowski, Anna Karpiewska, Mariola Turkiewicz, Tadeusz Dobosz
Hunting shot are 1.2-10 mm diameter balls, usually made of lead alloys, forming a cluster projectile used in smoothbore hunting shotguns. Shot may also be used in pistol and revolver ammunition, in which it can constitute structural element of the projectile. Shot pellets may also be made of other materials and have other shapes. The aim of this paper is to aggregate information on the topic available from a number of different sources. It is hoped that such information will be useful for forensic ballistics experts...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28155987/-traumatic-basal-subarachnoid-haemorrhage-or-ruptured-brain-aneurysm-in-16-year-old-boy-case-report
#3
Rafał Skowronek, Mariusz Kobek, Zbigniew Jankowski, Ewa Zielińska-Pająk, Artur Pałasz, Joanna Pilch-Kowalczyk, Rafał Kwarta, Krystian Rygol, Michał Szczepański, Czesław Chowaniec
Traumatic basal subarachnoid haemorrhage (TBSAH) represents only 1.8% of all subarachnoid haemorrhage cases diagnosed during autopsy. This report presents such a case from the current practice of the authors. Sixteen-year-old boy was beaten by the aggressors. Suddenly he lost his consciousness and fall after he received a single blow in the neck. He was resuscitated immediately, but died at the scene. During the external examination we did not find any significant external injuries. Autopsy revealed large contusion of right sternocleidomastoid muscle...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28155986/-histopathological-changes-in-lungs-of-the-mountain-snow-avalanche-victims-and-its-potential-usefulness-in-determination-of-cause-and-mechanism-of-death
#4
Mariusz Kobek, Rafał Skowronek, Christian Jabłoński, Zbigniew Jankowski, Artur Pałasz
On 28 January 2003 snow avalanche in the Polish Tatras happened, in which 8 people died and 5 were injured. We tried to determine cause and manner of death in 6 fatal victims instead of advanced late post mortem changes in internal organs. Taking into consideration the circumstances of death, we paid special attention to histopathological examination of lungs, extended by Gomori's and AZAN staining. Pattern of the changes was similar to those observed in forensic medicine in cases of asphyxia due to airway obstruction and/or immobilization of chest and abdomen (Perthes' syndrome)...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28155985/-polymorphism-of-12-str-loci-included-in-the-investigator-hd-plex-kit-in-polish-population-of-lodz-region
#5
Rafał Wojtkiewicz, Beata Markiewicz, Maciej Jędrzejczyk, Renata Jacewicz
In this study Polish population data as well as efficiency parameters of 12 STR <i>loci</i> included in the Investigator HDplex set were presented. This set contains 9 systems not available in any other commercial multiplexes, ie.: D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325 and D21S2055. The evaluation was preformed based on DNA samples derived from 303 unrelated individuals living in Lodz region, central part of Poland. The obtained distribution of the genotypes is consistent with the assumptions of the Hardy and Weinberg equilibrium (HWE)...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28155984/-pattern-of-drug-abuse-in-addicts-self-referred-drug-rehabilitation-centers-in-khuzestan-province-iran-2014-2015
#6
Farkhondeh Jamshidi, Iraj Nazari, Hossein Tahmasbian Malayeri, Zahra Rahimi, Maria Cheraghi
AIM OF THE STUDY: We aimed to determine the pattern of drug use and the affective demographic factors in addicts in self-referred drug rehabilitation centers in Khuzestan province. MATERIAL AND METHODS: It was a cross-sectional descriptive and analytic study which has done on 4400 addicts in self-referred drug rehabilitation centers in Khuzestan province from March 2014 to March 2015. The collected data were analyzed in SPSS version 22 software. Mean and standard deviation were calculated for continuous variables and the frequency and percentage frequency were calculated for nominal and classified variables, and also t-test, χ2 and ANOVA were used to examine the relationships between variables...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28144932/atypical-stab-injury-suggesting-ritual-suicide
#7
Štěpánka Kučerová, Tomáš Vojtíšek, Petr Hejna
Sharp weapons have been one of the most common deadly tools in cases of homicide and suicide for a long time. Tentative incisions accompany the majority of suicides by sharp force and the absence of hesitation marks provokes questions about the manner of death. We present the bizarre fatality of a 41-year-old male with an isolated circumscribed incision on the right neck, where the body was found lying in a pool of blood in the shower of his apartment. The internal examination revealed a 4-cm-long channel-like defect running inwardly and downwardly along the right sternocleidomastoid region...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28144931/-analysis-of-the-nature-of-injuries-in-victims-of-fall-from-height
#8
Magdalena E Kusior, Katarzyna Pejka, Michał Knapik, Nadja Sajuk, Szymon Kłaptocz, Tomasz Konopka
AIM OF STUDY: To assess the types and extent of injuries sustained by victims of fall from height depending on the height of fall. MATERIAL AND METHODS: The study included 338 bodies of victims of fatal falls from different heights (from the 1st to 10th floors) who were subjected to medico-legal autopsy at the Department of Forensic Medicine, Jagiellonian University Medical College, between 1995 and 2014. For each individual, selected data were collected including gender, age, body height, injury types and presence of alcohol or other intoxicants in blood...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28144930/-coii-long-fragment-reliability-in-characterisation-and-classification-of-forensically-important-flies
#9
Sanaa M Aly, Shereen M Mahmoud
INTRODUCTION: Molecular identification of collected flies is important in forensic entomological analysis guided with accurate evaluation of the chosen genetic marker. The selected mitochondrial DNA segments can be used to properly identify species. The aim of the present study was to determine the reliability of the 635-bp-long cytochrome oxidase II gene (COII) in identification of forensically important flies. MATERIAL AND METHODS: Forty-two specimens belonging to 11 species (Calliphoridae: Chrysomya albiceps, C...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28144929/-population-database-on-d1s1656-d2s441-d2s1338-d3s1358-d8s1179-d10s1248-d22s1045-d12s391-d16s539-d18s51-d19s433-d21s11-fga-th01-vwa-loci-included-in-ngm-system-based-on-one-thousand-unrelated-individuals-from-lodz-region-of-central-poland
#10
Renata Jacewicz, Beata Markiewicz, Rafał Wojtkiewicz, Maciej Jędrzejczyk, Jarosław Berent
A population data obtained on the basis of sample of 1000 unrelated individuals of Polish ancestry living in Lodz region of Central Poland with use of fluorescent multiplex-PCR and capillary electrophoresis were presented. Evaluation included 15 polymorphic loci DNA - STR from NGM multiplex-PCR set, ie. D1S1656, D2S441, D2S1338, D3S1358, D8S1179, D10S1248, D12S391, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, TH01, vWA. The allele frequency distribution and crucial statistical parameters for the investigated markers and the whole set were calculated...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/28144928/-estimation-of-postmortem-interval-using-vitreous-potassium-levels-in-cases-of-fatal-road-traffic-collision
#11
Suzanne N Foster, Paul R Smith, Michael Biggs, Guy N Rutty, Frances E Hollingbury, Stephen R Morley
AIM OF THE STUDY: To produce a formula that can accurately predict postmortem interval (PMI) based on vitreous potassium levels using road traffic collision fatalities. MATERIAL AND METHODS: Vitreous humour samples were taken from 78 individuals who had died following road traffic collisions between 2010 and 2015. Samples were obtained from both eyes and were sent for on-site analysis. Measurement of potassium was by an indirect ion-specific electrode Siemens diagnostics ADVIA 2400 chemistry system...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669344/first-successful-dna-isolation-and-profiling-from-bone-using-an-approach-that-is-non-destructive-toward-bone-surface
#12
D Pluta, A Lebioda, A Jonkisz, T Dobosz
Commonly used destructive DNA isolation techniques consist of pulverization which leads to the complete destruction of smaller bones or irreversible damage to larger bones through the cutting of extensive fragments. The procedure is totally unacceptable when handling bones which are valuable for anthropological or religious reasons, as museum exhibits or due to emotional factors. Since the Department's team has already resolved this problem in the case of human teeth (A. Pilecka), efforts have been undertaken to develop a similar non-destructive technique for the isolation of DNA from human bones...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669343/hunting-shot-evolution-of-manufacturing-technology
#13
P Bochyński, M Kuliczkowski, A Karpiewska, M Turkiewicz, T Dobosz
Hunting shot are 1.2-10 mm diameter balls, usually made of lead alloys, forming a cluster projectile used in smoothbore hunting shotguns. Shot may also be used in pistol and revolver ammunition, in which it can constitute structural element of the projectile. Shot pellets may also be made of other materials and have other shapes. The aim of this paper is to aggregate information on the topic available from a number of different sources. It is hoped that such information will be useful for forensic ballistics experts...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669342/traumatic-basal-subarachnoid-haemorrhage-or-ruptured-brain-aneurysm-in-16-year-old-boy-case-report
#14
R Skowronek, M Kobek, Z Jankowski, E Zielińska-Pająk, A Pałasz, J Pilch-Kowalczyk, R Kwarta, K Rygol, M Szczepański, C Chowaniec
Traumatic basal subarachnoid haemorrhage (TBSAH) represents only 1.8% of all subarachnoid haemorrhage cases diagnosed during autopsy. This report presents such a case from the current practice of the authors. Sixteen-year-old boy was beaten by the aggressors. Suddenly he lost his consciousness and fall after he received a single blow in the neck. He was resuscitated immediately, but died at the scene. During the external examination we did not find any significant external injuries. Autopsy revealed large contusion of right sternocleidomastoid muscle...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669341/histopathological-changes-in-lungs-of-the-mountain-snow-avalanche-victims-and-its-potential-usefulness-in-determination-of-cause-and-mechanism-of-death
#15
M Kobek, R Skowronek, C Jabłoński, Z Jankowski, A Pałasz
On 28 January 2003 snow avalanche in the Polish Tatras happened, in which 8 people died and 5 were injured. We tried to determine cause and manner of death in 6 fatal victims instead of advanced late post mortem changes in internal organs. Taking into consideration the circumstances of death, we paid special attention to histopathological examination of lungs, extended by Gomori's and AZAN staining. Pattern of the changes was similar to those observed in forensic medicine in cases of asphyxia due to airway obstruction and/or immobilization of chest and abdomen (Perthes' syndrome)...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669340/polymorphism-of-12-str-loci-included-in-the-investigator-hd-plex-kit-in-polish-population-of-lodz-region
#16
R Wojtkiewicz, B Markiewicz, M Jędrzejczyk, R Jacewicz
In this study Polish population data as well as efficiency parameters of 12 STR loci included in the Investigator HDplex set were presented. This set contains 9 systems not available in any other commercial multiplexes, ie.: D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325 and D21S2055. The evaluation was preformed based on DNA samples derived from 303 unrelated individuals living in Lodz region, central part of Poland. The obtained distribution of the genotypes is consistent with the assumptions of the Hardy and Weinberg equilibrium (HWE)...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27669339/pattern-of-drug-abuse-in-addicts-self-referred-drug-rehabilitation-centers-in-khuzestan-province-iran-2014-2015
#17
F Jamshidi, I Nazari, Z Rahimi, M Cheraghi
AIM OF THE STUDY: We aimed to determine the pattern of drug use and the affective demographic factors in addicts in self-referred drug rehabilitation centers in Khuzestan province. MATERIAL AND METHODS: It was a cross-sectional descriptive and analytic study which has done on 4400 addicts in self-referred drug rehabilitation centers in Khuzestan province from March 2014 to March 2015. The collected data were analyzed in SPSS version 22 software. Mean and standard deviation were calculated for continuous variables and the frequency and percentage frequency were calculated for nominal and classified variables, and also t-test, χ2 and ANOVA were used to examine the relationships between variables...
2016: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27543959/next-generation-sequencing-ngs-a-golden-tool-in-forensic-toolkit
#18
S M Aly, D M Sabri
The DNA analysis is a cornerstone in contemporary forensic sciences. DNA sequencing technologies are powerful tools that enrich molecular sciences in the past based on Sanger sequencing and continue to glowing these sciences based on Next generation sequencing (NGS). Next generation sequencing has excellent potential to flourish and increase the molecular applications in forensic sciences by jumping over the pitfalls of the conventional method of sequencing. The main advantages of NGS compared to conventional method that it utilizes simultaneously a large number of genetic markers with high-resolution of genetic data...
2015: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27543958/application-of-multi-phase-postmortem-ct-angiography-in-the-investigation-of-vascular-pathology-and-modified-vascular-anatomy-a-special-case-of-vascular-patchwork
#19
S Sabatasso, F Doenz, A Bass, K Michaud, P Mangin, S Grabherr
Multi-phase postmortem CT-angiography (MPMCTA) is used routinely for investigating cases of traumatic and natural death at the University Centre of Legal Medicine, Lausanne-Geneva. Here, we report the case of a patient affected by Leriche syndrome, with a history of numerous cardiovascular interventions, including an axillobifemoral bypass. The multiple cardiovascular changes presented by the patient were visualised by this relatively new technique and they were shown not to be related to the cause of death...
2015: Archiwum Medycyny Sa̧dowej i Kryminologii
https://www.readbyqxmd.com/read/27543957/dangers-resulting-from-dna-profiling-of-biological-materials-derived-from-patients-after-allogeneic-hematopoietic-stem-cell-transplantation-allo-hsct-with-regard-to-forensic-genetic-analysis
#20
R Jacewicz, K Lewandowski, J Rupa-Matysek, M Jędrzejczyk, J Berent
The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient's blood was identical with the donor's in the majority of cases...
2015: Archiwum Medycyny Sa̧dowej i Kryminologii
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