journal
MENU ▼
Read by QxMD icon Read
search

Cellular Immunology

journal
https://www.readbyqxmd.com/read/28622785/comprehensive-long-non-coding-rna-expression-profiling-reveals-their-potential-roles-in-systemic-lupus-erythematosus
#1
Lian-Ju Li, Wei Zhao, Sha-Sha Tao, Jun Li, Shu-Zhen Xu, Jie-Bing Wang, Rui-Xue Leng, Yin-Guang Fan, Hai-Feng Pan, Dong-Qing Ye
Long non-coding RNAs can regulate gene transcription, modulate protein function, and act as competing endogenous RNA. Yet, their roles in systemic lupus erythematosus remain to be elucidated. We determined the expression profiles of lncRNAs in T cells of SLE patients and healthy controls using microarrays. Up to 1935 lncRNAs and 1977 mRNAs were differentially expressed. QRT-PCR showed downregulated uc001ykl.1 and ENST00000448942 in SLE patients. Expression of uc001ykl.1 correlated with erythrocyte sedimentation rate (ESR) and C-reactive protein, whereas ENST00000448942 level correlated with ESR and anti-Sm antibodies...
June 10, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28606360/sex-differences-in-regulatory-cells-in-experimental-stroke
#2
Hilary A Seifert, Gil Benedek, Jian Liang, Ha Nguyen, Gail Kent, Arthur A Vandenbark, Julie A Saugstad, Halina Offner
Stroke is the leading cause of disability in the United States. Sex differences, including smaller infarcts in females and greater involvement of immune-mediated inflammation in males may affect the efficacy of immune-modulating interventions. To address these differences, we sought to identify distinct stroke-modifying mechanisms in female vs. male mice. The current study demonstrated smaller infarcts and increased levels of regulatory CD19(+)CD5(+)CD1d(hi) B10 cells as well as anti-inflammatory CD11b(+)CD206(+) microglia/macrophages in the ipsilateral vs...
June 8, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28602279/an-elisa-immunoassay-employing-a-conserved-leishmania-hypothetical-protein-for-the-serodiagnosis-of-visceral-and-tegumentary-leishmaniasis-in-dogs-and-humans
#3
Ana Maria R S Carvalho, Lourena E Costa, Beatriz C S Salles, Thaís T O Santos, Fernanda F Ramos, Mariana P Lima, Miguel A Chávez-Fumagalli, Bruna T Silvestre, Áquila S B Portela, Bruno M Roatt, Julia A G Silveira, Denise U Gonçalves, Danielle F Magalhães-Soares, Mariana C Duarte, Daniel Menezes-Souza, Eduardo A F Coelho
In the present study, a conserved Leishmania hypothetical protein, namely LiHypA, was evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans. This protein showed a high amino acid sequence homology between viscerotropic and cutaneotropic Leishmania species. An enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant antigen (rLiHypA), in addition to the A2 protein and two parasite antigenic preparations, which were used as controls. Regarding human diagnosis, results showed that rLiHypA was more sensitive and specific than ELISA-L...
June 8, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28595857/direct-control-of-b-cells-by-tregs-an-opportunity-for-long-term-modulation-of-the-humoral-response
#4
REVIEW
Elizabeth Weingartner, Amit Golding
Tregs are believed to be important for maintaining self-tolerance and immune homeostasis. It is well established that Tregs maintain self-tolerance and immune homeostasis by directly suppressing and modulating the effector function of T cells. However, there are a small number of studies that suggest Tregs also directly suppress and modulate B cells. Herein we review the literature that has investigated direct action of Tregs on B cell effector function. This area of study is intriguing because it suggests Tregs may influence long-lived humoral immunity...
June 1, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28601359/osteopontin-exacerbates-pseudomonas-aeruginosa-induced-bacteremia-in-mice
#5
Zhenghao Piao, Haiying Yuan
Osteopontin (OPN) is a multifunctional protein involved in various pathophysiological processes. However, the role of OPN in Pseudomonas aeruginosa-related sepsis is not yet clear. Here, we found that OPN expression was elevated in plasma and spleen samples from P. aeruginosa-infected mice. To determine the function of OPN in sepsis, we used wild-type (WT) and OPN-knockout (KO) mice with P. aeruginosa-induced bacteremia. We found that OPN-KO mice exhibited reduced mortality compared with WT mice and that OPN exacerbated spleen bleeding and functional impairment...
May 30, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28535870/age-associated-b-cells-abc-inhibit-b-lymphopoiesis-and-alter-antibody-repertoires-in-old-age
#6
Richard L Riley, Kelly Khomtchouk, Bonnie B Blomberg
With old age (∼2y old), mice show substantial differences in B cell composition within the lymphoid tissues. In particular, a novel subset of IgM(+) CD21/35(lo/-) CD23(-) mature B cells, the age-associated B cells or ABC, increases numerically and proportionately. This occurs at the expense of other B cell subsets, including B2 follicular B cells in spleen and recirculating primary B cells in bone marrow. Our studies suggest that ABC have a distinctive antibody repertoire, as evidenced by relatively high reactivity to the self-antigens phosphorylcholine (PC) and malondialdehyde (MDA)...
April 26, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28457482/aging-effects-on-t-bet-expression-in-human-b-cell-subsets
#7
Daniela Frasca, Alain Diaz, Maria Romero, Francesca D'Eramo, Bonnie B Blomberg
In order to compare human and mouse B cell subset markers, we evaluated T-bet expression in human B cell subsets from individuals of different ages. We found T-bet expressed in unstimulated memory more than naïve B cells, and more in young individuals. TLR7 stimulation up-regulated T-bet in all B cell subsets from young and elderly individuals, and more in the elderly. By fold-increase the best effect was seen in subsets of the elderly and especially in those that undergo class switch (naïve and IgM). We also evaluated CD11c expression, as T-bet+CD11c+ B cells are expanded in healthy elderly individuals and also in patients with autoimmunity...
April 24, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28433198/tumor-promoting-role-of-anti-tumor-macrophages-in-tumor-microenvironment
#8
REVIEW
Kuntal Kanti Goswami, Tithi Ghosh, Sarbari Ghosh, Madhurima Sarkar, Anamika Bose, Rathindranath Baral
Recent advances in tumor biology demand detailed analysis of the complex interaction of tumor cells with their adjacent microenvironment (tumor stroma) to understand the various mechanisms involved in tumor growth and metastasis. Mononuclear phagocytes or macrophages, a type of innate immune cells, defend the organism against infection and injury. On the otherhand, tumor associated macrophages (TAMs) constitute a significant part of the tumor-infiltrating immune cells, have been linked to the growth, angiogenesis, and metastasis of a variety of cancers, most likely through polarization of TAMs to the M2 (alternative) phenotype...
April 13, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28413062/assessment-of-mucosal-integrity-by-quantifying-neutrophil-granulocyte-influx-in-murine-models-of-acute-intestinal-injury
#9
Julius Clemens Fischer, Alexander Wintges, Tobias Haas, Hendrik Poeck
Intact epithelial body surfaces represent physical barriers which protect the organism from invading pathogens and loss of nutrients. Barrier malfunction is closely linked to disorders such as inflammatory bowel disease and graft-versus-host disease. In fact, several pharmacological or radiobiological therapeutic strategies have side effects that affect epithelial surfaces. In this context, assays that accurately assess epithelial barrier integrity in patients and animal models are crucial to create a better understanding of the mechanisms leading to disease or limiting therapeutic approaches due to barrier disruption...
April 11, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28391993/sodium-azide-suppresses-lps-induced-expression-mcp-1-through-regulating-i%C3%AE%C2%BAb%C3%AE-and-stat1-activities-in-macrophages
#10
Cho-Yi Park, Jae-Nyoung Heo, Kyoungho Suk, Won-Ha Lee
Sodium azide (NaN3) is a chemical compound with multiple toxic effects on vascular and neuronal systems, causing hypotension and neurotoxicity, respectively. In order to test its effects on the immune system, human and mouse macrophage-like cell lines were treated with nontoxic doses of NaN3 and the changes in LPS-induced inflammatory activation was measured. Interestingly, the LPS-induced expression of monocyte chemoattractant protein (MCP)-1 was suppressed by NaN3 without affecting the expression of IL-8 and TNF-α...
April 4, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28433199/glycoprotein-non-metastatic-melanoma-protein-b-gpnmb-is-highly-expressed-in-macrophages-of-acute-injured-kidney-and-promotes-m2-macrophages-polarization
#11
Letian Zhou, Hui Zhuo, Huiyu Ouyang, Yexin Liu, Fang Yuan, Lin Sun, Fuyou Liu, Hong Liu
Acute kidney injury (AKI) is an increasingly common disorder that is strongly linked to short- and long-term morbidity and mortality. During AKI process, macrophages, one of the important immune response cells, can polarize into M1 and M2 subtype from M0 subtype. It is well-known that M1 macrophages play a pro inflammatory role while M2 macrophages play an anti-inflammatory role. Glycoprotein non-metastatic melanoma protein b (Gpnmb) is a glycosylated transmembrane protein highly expressed in numerous cells, including osteoblasts, dendritic cells and macrophages...
March 31, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28372797/unique-high-and-homogenous-surface-expression-of-the-transferrin-receptor-cd71-on-murine-plasmacytoid-dendritic-cells-in-different-tissues
#12
Anne Lippitsch, Yuri Chukovetskyi, Nelli Baal, Gregor Bein, Holger Hackstein
Plasmacytoid dendritic cells (pDC) are of increasing interest in cancer vaccine development, but many functions of these highly specialized, multifaceted cells are poorly understood. The transferrin receptor CD71 has also been suggested to function as an antigen uptake receptor on professional antigen-presenting cells. In this study, we employed multiparameter flow cytometry to investigate CD71 expression on various leukocyte subsets, including DC subsets, granulocytes, macrophages, T and B lymphocytes, γδ T cells, and natural killer cells...
March 29, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28372798/stimulation-of-hiv-specific-t-cell-clonotypes-using-allogeneic-hla
#13
Coral-Ann Almeida, Paula van Miert, Kane O'Driscoll, Yvonne M Zoet, Abha Chopra, Mark Watson, Dianne de Santis, Campbell Witt, Mina John, Frans H J Claas, Lloyd J D'Orsogna
We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested...
March 28, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28576260/regulation-of-bone-mass-by-the-gut-microbiota-is-dependent-on-nod1-and-nod2-signaling
#14
Claes Ohlsson, Giulia Nigro, Ivo Gomperts Boneca, Fredrik Bäckhed, Philippe Sansonetti, Klara Sjögren
Germ-free (GF) mice have increased bone mass that is normalized by colonization with gut microbiota (GM) from conventionally raised (CONV-R) mice. To determine if innate immune signaling pathways mediated the effect of the GM, we studied the skeleton of GF and CONV-R mice with targeted inactivation of MYD88, NOD1 or NOD2. In contrast to WT and Myd88(-/-) mice, cortical bone thickness in mice lacking Nod1 or Nod2 was not increased under GF conditions. The expression of Tnfα and the osteoclastogenic factor Rankl in bone was reduced in GF compared to CONV-R WT mice but not in Nod1(-/-) or Nod2(-/-) mice indicating that the effect of the GM to increase Tnfα and Rankl in bone and to reduce bone mass is dependent on both NOD1 and NOD2 signaling...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28511921/myeloperoxidase-a-new-player-in-autoimmunity
#15
REVIEW
Anna Strzepa, Kirkwood A Pritchard, Bonnie N Dittel
Myeloperoxidase (MPO) is the most toxic enzyme found in the azurophilic granules of neutrophils. MPO utilizes H2O2 to generate hypochlorous acid (HClO) and other reactive moieties, which kill pathogens during infections. In contrast, in the setting of sterile inflammation, MPO and MPO-derived oxidants are thought to be pathogenic, promoting inflammation and causing tissue damage. In contrast, evidence also exists that MPO can limit the extent of immune responses. Elevated MPO levels and activity are observed in a number of autoimmune diseases including in the central nervous system (CNS) of multiple sclerosis (MS) and the joints of rheumatoid arthritis (RA) patients...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28499490/frequent-inoculations-with-radiation-attenuated-sporozoite-is-essential-for-inducing-sterile-protection-that-correlates-with-a-threshold-level-of-plasmodia-liver-stage-specific-cd8-t-cells
#16
Hardik Patel, Naveen Yadav, Rajesh Parmar, Satish Patel, Agam P Singh, Neeta Shrivastava, Sarat K Dalai
Whole sporozoite vaccine (WSV) is shown to induce sterile protection that targets Plasmodium liver-stage infection. There are many underlying issues associated with induction of effective sterile protracted protection. In this study, we have addressed how the alterations in successive vaccine regimen could possibly affect the induction of sterile protection. We have demonstrated that the pattern of vaccination with RAS (radiation attenuated sporozoites) induces varying degrees of protection among B6 mice. Animals receiving four successive doses generated 100% sterile protection...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28477840/leukotriene-receptor-expression-in-mast-cells-is-affected-by-their-agonists
#17
Justyna Agier, Sylwia Różalska, Karolina Wódz, Ewa Brzezińska-Błaszczyk
The effects of LTs are mediated by GPCRs: cysLTs interact with CYSLTR1, CYSLTR2, or GPR17, and LTB4 acts via BLT1R or BLT2R. Data relating to the presence of these receptors in mature tissue mast cells are not entirely known. By confocal microscopy with image analyses and flow cytometry, we established that native rat mast cells isolated from peritoneal cavity constitutively express all studied receptors. Moreover, we clearly documented that LTs by themselves can influence their own receptor expression. Low concentrations of LTs induce translocation of LT receptors from cell interior to plasma membrane, which can lead to increased mast cell responsiveness to LT stimulation...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28476343/immune-responses-to-fungal-aeroallergen-in-heligmosomoides-polygyrus-infected-mice-vary-by-age
#18
Nopporn Apiwattanakul, Maneesha Palipane, Amali Eashani Samarasinghe
Parasite infections in the developing world have been considered to promote resistance to immune-mediated diseases such as asthma. Mouse studies have shown that helminths and their products reduce the development of allergic asthma. Since epidemiologic studies that show similar protection are in relation to geohelminth infections that occur in early life, we hypothesized that the parasite-mediated protection against asthma may differ by age. Mice infected with Heligmosomoides polygyrus at 3-weeks of age had similar asthma phenotype compared to mice infected at 28-weeks of age wherein airway eosinophilia was unaltered but tissue inflammation and GC metaplasia were reduced...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28438315/tgf-%C3%AE-1-improving-abnormal-pregnancy-outcomes-induced-by-toxoplasma-gondii-infection-regulating-nkg2d-dap10-and-killer-subset-of-decidual-nk-cells
#19
Xiaoyan Xu, Jian Zhang, Shaowei Zhan, Zhidan Li, Xianbing Liu, Haixia Zhang, Yuzhu Jiang, Xuemei Hu
Our current aim was to investigate whether injection of TGF-β1 played an important role in improving abnormal pregnancy outcomes with T. gondii infection and how the TGF-β1 regulated. Results showed that TGF-β1 exhibited improved pregnancy outcomes induced by T. gondii infection. dNK cytotoxicity was increased with T. gondii infection while decreased with TGF-β1 treatment. dNK cytotoxicity related NKG2D/DAP10 expression, perforin, granzyme, IFN-γ and killer subsets were all increased with T. gondii infection while decreased after TGF-β1 treatment...
July 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28438314/progression-of-experimental-autoimmune-encephalomyelitis-is-associated-with-up-regulation-of-major-sodium-transporters-in-the-mouse-kidney-cortex-under-a-normal-salt-diet
#20
Xiaoming Zhou, Balamurugan Packialakshmi, Yao Xiao, Saule Nurmukhambetova, Jason R Lees
Recent demonstrations of exacerbation of experimental autoimmune encephalomyelitis (EAE) by high salt diets prompted us to study whether EAE stimulated Na absorption by the renal cortex, a primary regulatory site for Na balance, even under a normal NaCl diet. We found that as EAE progressed from mild to severe symptoms, there were parallel increases in the protein abundance of NHE3 and αENaC and the Na,K-ATPase activity with an affiliated elevation of its β1-subunit protein. These effects are associated with increases in the protein levels of the well-known regulators SGK1 and scaffold NHERF2, and phosphorylation of ERK1/2...
July 2017: Cellular Immunology
journal
journal
25183
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"