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Andrologia

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https://www.readbyqxmd.com/read/29896906/assessment-of-seminal-granulysin-in-infertile-men-with-varicocele
#1
Nancy W Mikhael, Asmaa M El-Refaie, Jehan H Sabry, Essam M Akl, Amal Y Habashy, Taymour Mostafa
Varicocele has a common association with male infertility, but its exact role is still debated. Apoptosis has been suggested as one of the mechanisms of varicocele-associated infertility. Granulysin is a molecule that plays a role in apoptosis with no previous study about its role in male infertility. This case-controlled study aimed to assess seminal plasma granulysin level in infertile patients with varicocele. This study involved 90 men that were allocated into fertile normozoospermic men (n = 20), infertile men without varicocele (n = 30) and infertile men with varicocele (n = 40)...
June 13, 2018: Andrologia
https://www.readbyqxmd.com/read/29882361/influence-of-human-sperm-origin-testicular-or-ejaculated-on-embryo-morphokinetic-development
#2
Filomena Scarselli, Valentina Casciani, Elisabetta Cursio, Saverio Muzzì, Alessandro Colasante, Simona Gatti, Maria Chiara Greco, Pierfrancesco Greco, Maria Giulia Minasi, Ermanno Greco
In this retrospective observational study (October 2014 - July 2016), the impact of sperm origin on embryo morphokinetics and on clinical outcomes after intracytoplasmic sperm injection was evaluated. The developmental kinetics of embryos obtained either with testicular sperm (40 cycles; testicular sperm group) or with thawed donor sperm (26 cycles; donor sperm group) was analysed up to day-3 of culture with a time-lapse incubation system. In the testicular sperm group, all patients were affected by nonobstructive azoospermia...
June 8, 2018: Andrologia
https://www.readbyqxmd.com/read/29876952/can-melissa-officinalis-improve-chromatin-structure-and-sperm-parameters-in-a-rat-model-of-varicocele
#3
Bijan Rezakhaniha, Reza Heidari, Mehdi Abbasi
Excessive production of reactive oxygen species is a central mechanism in the pathology of varicocele; Melissa officinalis (MO) is a medicinal plant from the Lamiaceae family that has antioxidant activity. In this study, we have investigated the potential therapeutic effect of MO on sperm parameters and chromatin structure in varicocelised rat. Male Wistar rats were randomly assigned to control, sham, varicocele, MO treatment and placebo groups. Rats in varicocele, MO treatment and placebo groups underwent left varicocele induction...
June 6, 2018: Andrologia
https://www.readbyqxmd.com/read/29862548/ameliorating-effect-of-kisspeptin-10-on-methotrexate-induced-sperm-damages-and-testicular-oxidative-stress-in-rats
#4
Mehmet Güvenç, Mesut Aksakal
The purpose of this study was to determine the kisspeptin-10 (Kiss) administration on the damages in testicular oxidant-antioxidant system, reproductive organ weights and some spermatological characteristics resulted from methotrexate (MTX) exposure. Group 1 (n:6) received saline only; group 2 (n:6) received 50 nmol/kg kisspeptin-10 for 10 days; group 3 (n:10) received single-dose methotrexate 20 mg/kg; and group 4 (n:10) received MTX 20 mg/kg single dose and, after 3 days, received kisspeptin-10, 50 nmol/kg, lasted for 10 days by intraperitoneal injection...
June 3, 2018: Andrologia
https://www.readbyqxmd.com/read/29862542/effects-of-perfluorooctanoic-acid-exposure-during-pregnancy-on-the-reproduction-and-development-of-male-offspring-mice
#5
Pengyan Song, Danyang Li, Xiaodan Wang, Xiuhui Zhong
This study was conducted to explore the effects of maternal exposure to perfluorooctanoic acid (PFOA) on reproduction and development of male offspring mice. Pregnant mice were given 1, 2.5 or 5 mg/kg BW PFOA daily by gavage during gestation. The results showed that the survival number of offspring mice at weaning was significantly decreased. There were no differences in the testicular index of offspring mice between PFOA exposure groups and non-PFOA group. Maternal exposure to PFOA reduced the level of testosterone in the male offspring mice on PND 21 (p < 0...
June 3, 2018: Andrologia
https://www.readbyqxmd.com/read/29862537/the-clinical-and-neonatal-outcomes-after-stimulation-of-immotile-spermatozoa-using-spermagic-medium
#6
Yi-Fan Gu, Qin-Wei Zhou, Shuo-Ping Zhang, Chang-Fu Lu, Fei Gong, Yun Shi, Guang-Xiu Lu, Ge Lin
To evaluate the efficiency and safety of SperMagic medium on stimulating the immotile spermatozoa in testicular sperm extraction (TESE) and absolute asthenozoospermia, 96 patients with TESE and 106 patients with absolute asthenozoospermia were enrolled in this study. The motile spermatozoa were detected in 47 TESE patients and 68 absolute asthenozoospermia and these patients were assigned to control group. The immotile spermatozoa in 49 TESE patients and 34 absolute asthenozoospermia were stimulated with SperMagic medium...
June 3, 2018: Andrologia
https://www.readbyqxmd.com/read/29808481/association-between-male-infertility-and-seminal-plasma-levels-of-growth-hormone-and-insulin-like-growth-factor-1
#7
Mara Simopoulou, Anastassios Philippou, Evangelos Maziotis, Konstantinos Sfakianoudis, Nikolaos Nitsos, Panagiotis Bakas, Roxane Tenta, Evangelos Zevolis, Konstantinos Pantos, Michael Koutsilieris
Growth hormone (GH) and insulin-like growth factor 1 (IGF-1) have been proposed to play a pivotal role in male infertility due to their anabolic effects. The aim of this study was to investigate possible associations between seminal plasma levels of GH and IGF-1 and sperm parameters. Fifty men participated in this study. Semen analysis was performed, while cell-free seminal plasma was collected following sperm centrifugation. Seminal plasma concentrations of IGF-1 and GH were determined by enzyme-linked immunosorbent assay (ELISA)...
May 28, 2018: Andrologia
https://www.readbyqxmd.com/read/29806220/vitamin-e-attenuates-nicotine-and-noise-induced-reproductive-impairment-in-male-albino-wistar-rats
#8
S A Bisong, I E Ukoh, V U Nna, P E Ebong
Previous studies showed that exposure to stress or nicotine induced reproductive impairment in male rats. Here, we assessed the effect of an antioxidant (vitamin E) on nicotine-, stress- and nicotine + stress-induced reproductive impairment in male rats. Forty-eight male albino Wistar rats were divided into eight groups as follows; control, stress (generator noise 90-120 dB, 8 hr/day), nicotine (1.5 mg kg-1 day-1 ), nicotine + stress, vitamin E (100 mg kg-1 day-1 ), stress + vitamin E, nicotine + vitamin E and stress + nicotine + vitamin E...
May 28, 2018: Andrologia
https://www.readbyqxmd.com/read/29806204/evaluation-of-the-sysmex-uf-1000i-system-as-an-alternative-for-the-screening-of-genital-tract-inflammation-in-male-infertility-patients
#9
Yonghua Wu, Xu Yang, Haining Wang, Tiancheng Wang
The number of white blood cell (WBC) in semen is an important indicator of genital tract inflammation in male infertility. The peroxidase assay is the recommended reference method for seminal WBC counting. However, it is time-consuming and may cause relatively heavy workload in daily routine. Meanwhile, the main component in the reagent of peroxidase test is harmful to human and the environment. In this study, we evaluated the analytical performance of the Sysmex UF-1000i that is a urine flow cytometer as a screening tool for genital tract infection in male infertility patients through the counting of seminal WBC...
May 28, 2018: Andrologia
https://www.readbyqxmd.com/read/29806152/reduction-in-peyronie-s-like-plaque-size-using-a-vacuum-erection-device-in-a-rat-model-of-peyronie-s-disease-via-the-tgf-%C3%AE-smad-signalling-pathway
#10
Jinhong Li, Shuzhen Wang, Feng Qin, Mengli Zhu, Xuanhe You, Changjing Wu, Fudong Fu, Jiuhong Yuan
Peyronie's disease (PD) is a fibrotic disorder of the tunica albuginea (TA). This study aimed to determine the therapeutic effects of a vacuum erection device (VED) in an animal model of PD and explore the possible mechanisms. Twenty-seven male Sprague-Dawley rats were used. The sham group (group A) (N = 9) received a 50-μl-saline vehicle injection into the TA, while the remaining 18 rats (groups B and C) received a TGF-β1 injection into the TA. The treatment group (group C) underwent VED therapy for 10 days after the TGF-β1 injection...
May 28, 2018: Andrologia
https://www.readbyqxmd.com/read/29797334/pelvic-mass-schwannoma-of-the-left-seminal-vesicle
#11
Jun Huang, Zhouping Cheng, Ronggang Li, Nan Yao, Jiazheng Cao, Shuo Deng, Ronghai Wu
Schwannomas rarely occur in seminal vesicles. Here, we report a schwannoma of the left seminal vesicle. A 55-year-old man presented no clinical symptoms, and a mass in the left region of the seminal vesicle was found incidentally in a medical examination. A computed tomography and magnetic resonance imaging of pelvic were obtained and revealed a 5.17 × 2.59 × 3.5 cm mass on the left seminal vesicle. Transrectal ultrasound-guided seminal biopsy revealed a diagnosis of seminal vesical schwannoma. Laparoscopic resection of the tumour was performed...
May 24, 2018: Andrologia
https://www.readbyqxmd.com/read/29785804/can-scsa-and-tunel-forecast-apoptosis-related-motility-depletion-in-asthenozoospermia
#12
Z Moradian Fard, M Naghdi, P Salehi, A Ajami, M R Deemeh, M H Meshkibaf
This study is an attempt to determine the power of SCSA and TUNEL for the evaluation of apoptosis status and apoptosis-related motility depletion in Asthenozoospermia. Fifty-one semen samples from Asthenozoospermic and 20 samples from fertile men participated in this study. SCSA and TUNEL were applied for the assessment of DNA integrity by flow cytometry. Annexin V conjugated with FITC labelling and FLICA method were used for the assessment of externalisation of phosphatidylserine and spermatozoon with active Caspase 3 respectively...
May 21, 2018: Andrologia
https://www.readbyqxmd.com/read/29781510/tramadol-opioid-abuse-is-associated-with-a-dose-and-time-dependent-poor-sperm-quality-and-hyperprolactinaemia-in-young-men
#13
A G A Farag, M A Basha, S A Amin, N F Elnaidany, N G Elhelbawy, M M T Mostafa, S A Khodier, R A Ibrahem, R Z Mahfouz
Tramadol, one of the most commonly abused drugs in Middle East, impacts spermatogenesis and disturbs reproductive hormones in animal studies. We aimed to investigate tramadol impact on sperm quality and on levels of testosterone, prolactin and gonadotropins, in tramadol abusers (n = 30) to age-matched control (n = 30). Abusers had significantly low percentages of sperm motility, normal forms and vitality compared with control (95% CI -40.7 to -19.3, -13.5 to -9.3 and -31.9 to -9.7 respectively). Hypoandrogenism (95% CI -4...
May 21, 2018: Andrologia
https://www.readbyqxmd.com/read/29781095/gasoline-exhaust-damages-spermatogenesis-through-downregulating-%C3%AE-6-integrin-and-%C3%AE-1-integrin-in-the-rat-model
#14
L Luo, E Li, S Zhao, J Wang, Z Zhu, Y Liu, J Luo, Z Zhao
The health influence of air pollution has been an international public health concern. Increasing evidence has suggested that air pollution has been associated with decreased sperm quality. However, the underlying molecular mechanisms are still not fully elucidated. We aimed to verify whether gasoline exhaust leads to reproductive impairment by injuring spermatogonial stem cells and explore its underlying molecular mechanism. Twenty male Sprague-Dawley rats were randomly divided into two groups: the exposure group (n = 10) and the control group (n = 10)...
May 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29781087/is-%C3%AE-endorphin-significant-in-the-control-of-the-male-sexual-response
#15
Stefan Ückert, Armin J Becker, Andreas Bannowsky, Christian G Stief, Markus A Kuczyk
It has been assumed that β-endorphin, belonging to the family of opiodergic neuropeptides, might facilitate the inhibition of the male sexual response; however, its role in the control of the penile erectile tissue remains to be elucidated. This study aimed to evaluate in healthy men the course of β-endorphin in the systemic and cavernous blood through different stages of sexual arousal. Thirty-four (34) men were exposed to erotic stimuli to induce penile tumescence and rigidity. Blood was aspirated from the corpus cavernosum and a cubital vein during the penile conditions flaccidity, tumescence, rigidity and detumescence...
May 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29770471/protective-effects-of-the-hydroalcoholic-extract-of-fumaria-parviflora-on-testicular-injury-induced-by-torsion-detorsion-in-adult-rats
#16
M Shokoohi, H Shoorei, M Soltani, S-H Abtahi-Eivari, R Salimnejad, M Moghimian
This study was designed to determine the effects of daily oral administration (250 mg/kg) of the hydroalcoholic extract of Fumaria parviflora (FP) for 14 days on the sperm parameters, oxidative stress parameters, serum testosterone levels, expression of Bax and Bcl-2 genes, and apoptosis index of germ cells after testicular torsion-detorsion (ischaemia-reperfusion, IR) injury model in rats. Twenty-eight adult male Wistar rats were divided randomly into four groups of seven each: sham operation, torsion-detorsion (TD), TD plus the hydroalcoholic extract FP (TDFP) and only FP without TD application (FP)...
May 16, 2018: Andrologia
https://www.readbyqxmd.com/read/29770466/comparative-evaluation-of-the-effects-of-withania-somnifera-with-pentoxifylline-on-the-sperm-parameters-in-idiopathic-male-infertility-a-triple-blind-randomised-clinical-trial
#17
R Nasimi Doost Azgomi, H Nazemiyeh, H Sadeghi Bazargani, S M B Fazljou, F Nejatbakhsh, A Moini Jazani, Y Ahmadi AsrBadr, A Zomorrodi
In this triple-blind randomised clinical trial, we compared the effects of Withania somnifera and pentoxifylline on the sperm parameters in idiopathic male infertility. One hundred infertile male patients were randomly allocated into either W. somnifera or pentoxifylline groups. Patients in the herbal group received six capsules containing 5 g/daily of W. somnifera root, and subjects in the pentoxifylline group received six capsules containing 800 mg/daily of pentoxifylline and placebo for 90 days. Sperm parameters were analysed at the beginning and end of the study...
May 16, 2018: Andrologia
https://www.readbyqxmd.com/read/29770464/chemically-characterised-extract-of-saraca-asoca-improves-the-sexual-function-in-male-wistar-rats
#18
M Gill, A Rai, M Kinra, S Sumalatha, C M Rao, S P Cheruku, R Devkar, N Kumar
In this study, methanolic extract of Saraca asoca bark was evaluated for its aphrodisiac potential using male and female Wistar albino rats. Male rats were dosed daily for 54 days at a dose of 100 mg/kg p.o. Sexual activity of male rats was assessed after 14, 28, 42 and 54 days of the study. Male rats were placed in a glass chamber lit with a dim red light (10W) followed by the introduction of sexually receptive female rats in a ratio of 1:1. Improvement in sexual behaviour of male rats was characterised by an increase in both mount frequency and intromission frequency and decrease or reduction in mount latency and intromission latency compared to normal control...
May 16, 2018: Andrologia
https://www.readbyqxmd.com/read/29761547/a-novel-combination-of-silane-coated-silica-colloid-with-hybrid-rna-extraction-protocol-and-rna-enrichment-for-downstream-applications-of-spermatozoal-rna
#19
K Vijayalakshmy, P Kumar, M Virmani, S Pawaria, N S Lalaji, P Sharma, R Rajendran, P S Yadav, D Kumar
Spermatozoa are specialised cells with low RNA content as compared to somatic cells. The suitable sperm RNA extraction and enrichment protocols for downstream applications are available for human, cattle, stallion and mouse but not for buffalo spermatozoa. Therefore, the present work was conducted to find out suitable colloidal solution for sperm purification and appropriate protocol for sperm RNA extraction and enrichment/amplification of RNA. For purification, we used PVP-coated silica colloidal solution (PVP-Si), silane-coated silica colloidal solution (Silane-Si) and iodixanol...
May 14, 2018: Andrologia
https://www.readbyqxmd.com/read/29761542/ameliorative-effect-of-resveratrol-on-testicular-oxidative-stress-spermatological-parameters-and-dna-damage-in-glyphosate-based-herbicide-exposed-rats
#20
F Avdatek, Y O Birdane, R Türkmen, H H Demirel
In this study, the reproductive impacts of being exposed to glyphosate (GLF) and the protective impacts of resveratrol (RES) were assessed in 28 Wistar male rats, which were equally separated into four groups. Control group were fed normal diet without GLF or RES, group II received normal feed containing 20 mg kg-1 daily-1 RES, group III received normal feed containing 375 mg kg-1 daily-1 GLF, and group IV received normal feed containing 375 mg kg-1 daily-1 GLF+20 mg kg-1 daily-1 RES. GLF administration decreased sperm motility, sperm plasma membrane integrity, glutathione level and superoxide dismutase in the testicular tissue of rats...
May 14, 2018: Andrologia
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