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Theriogenology

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https://www.readbyqxmd.com/read/28407867/the-relationship-between-mitochondrial-dna-copy-number-and-stallion-sperm-function
#1
Christa R Darr, Luis E Moraes, Richard E Connon, Charles C Love, Sheila Teague, Dickson D Varner, Stuart A Meyers
Mitochondrial DNA (mtDNA) copy number has been utilized as a measure of sperm quality in several species including mice, dogs, and humans, and has been suggested as a potential biomarker of fertility in stallion sperm. The results of the present study extend this recent discovery using sperm samples from American Quarter Horse stallions of varying age. By determining copy number of three mitochondrial genes, cytochrome b (CYTB), NADH dehydrogenase 1 (ND1) and NADH dehydrogenase 4 (ND4), instead of a single gene, we demonstrate an improved understanding of mtDNA fate in stallion sperm mitochondria following spermatogenesis...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407866/subclinical-endometritis-in-beef-cattle-in-early-and-late-postpartum-cytology-bacteriology-haptoglobin-and-test-strip-efficiency-to-evaluate-the-evolution-of-the-disease
#2
A Ricci, G Bonizzi, G Sarasso, S Gallo, A Dondo, S Zoppi, L Vincenti
In cows, detrimental effects on fertility are mainly caused by clinical and subclinical endometritis (SEM). As demonstrated in previous work, Piedmontese cattle are affected by a higher rate of infertility and presence of SEM. The objective of this study is to assess the pattern of SEM at 30 and 60 days postpartum by evaluating the correlation between uterine cytology and microbiology, analyzing SEM consequences on reproductive career and verifying the reliability of rising inflammatory proteins - haptoglobin and the test strip test...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407865/the-effect-of-dual-hemisphere-breeding-on-stallion-fertility
#3
S R Walbornn, C C Love, T L Blanchard, S P Brinsko, D D Varner
Breeding records were analyzed from 24 Thoroughbred stallions that were subjected to dual-hemisphere breeding (DH), including novice (first-year; NOV; n = 11) and experienced (EXP; n = 13) stallions. Fertility variables included seasonal pregnancy rate, pregnancy rate per cycle, and first-cycle pregnancy rate. In addition, values for book size, total number of covers, distribution of mare type (maiden, foaling, and barren) within a stallion's book, cycles per mare, and mare age were examined. Some data were also categorized by mare type (maiden-M, foaling-F, and barren-B)...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407864/birth-of-cloned-mice-from-vaginal-smear-cells-after-somatic-cell-nuclear-transfer
#4
Hiroki Kuwayama, Yoshiaki Tanabe, Teruhiko Wakayama, Satoshi Kishigami
Less invasive methods for donor cell collection will facilitate reproduction of wild animals using somatic-cell nuclear transfer. Stages of the estrous cycle in mice have long been studies using somatic cells that can be collected from vaginal walls using cotton tipped swabs in a relatively non-invasive manner. In this study, we examined the feasibility of these cells as sources of nuclei for somatic-cell cloning using nuclear transfer. Estrous cycles generally comprise proestrus, estrus, metestrus, and diestrus stages...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407863/reproductive-characteristics-of-female-white-tailed-deer-odocoileus-virginianus-in-the-midwestern-usa
#5
Michelle L Green, Amy C Kelly, Damian Satterthwaite-Phillips, Mary Beth Manjerovic, Paul Shelton, Jan Novakofski, Nohra Mateus-Pinilla
Knowledge of reproductive characteristics of wild populations is necessary to inform responsible management decisions that promote herd health. As management, goals, and free-ranging populations change over time and landscapes, updated knowledge of reproductive characteristics are needed to inform responsible management practices. We estimated reproductive characteristics of female white-tailed deer in Illinois, including pregnancy rate, litter size, fetal growth and fetal sex ratio. We found maternal age to have an important influence on several reproductive factors...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407862/lipid-profiles-of-follicular-fluid-from-cows-submitted-to-ovarian-superstimulation
#6
P H Santos, P K Fontes, F F Franchi, M F G Nogueira, K R A Belaz, A Tata, M N Eberlin, M J Sudano, C M Barros, A C S Castilho
Ovarian superstimulation with exogenous gonadotropins has been extensively used to produce in vivo-derived embryos for embryo transfer in cattle. This process modifies the antral follicle microenvironment and affects oocyte and embryo quality as well the differentiation of granulosa cells. Lipids play significant roles in the cell, such as energy storage, cell structure, and fine-tuning of the physical properties and functions of biological membranes. The phospholipid (PL) contents as well as the effects of superstimulatory treatments on the PL profile of follicular fluid from cows, however, remain unknown...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407861/influence-of-different-types-of-lamps-on-the-reproductive-development-of-male-japanese-quail-coturnix-coturnix-japonica
#7
Pâmela L Retes, Marcelo Espósito, Danusa G das Neves, Arabela G A Viana, Louise M Coelho, Manuel F Bobadilla-Mendez, Renata R Alvarenga, Edison J Fassani, Juliano V Peixoto, Márcio G Zangeronimo
Reproductive tract development during puberty is critical to reproductive performance, and the light is crucial in this process in birds. However, in male quail, there is little information on the effects of types of lamps, more specifically the wavelength emitted. Thus, the objective was to evaluate the effects of types of lamps on the reproductive performance of male Japanese quail (Coturnix coturnix japonica). Two hundred and forty male quail were exposed to six different types of lamp (incandescent, white fluorescent, or blue, white, red or green LED)...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407860/effects-of-mg132-on-the-in%C3%A2-vitro-development-and-epigenetic-modification-of-debao-porcine-somatic-cell-nuclear-transfer-embryos
#8
Kaiyuan Shen, Xiangping Li, Xiaoli Dai, Ping Wang, Sheng Li, Zhaocheng Xiong, Peifang Chen, Qingyou Liu, Deshun Shi
The present study was undertaken to examine the effect of MG132, a proteasome inhibitor, on the in vitro development, zygotic genome activation (ZGA) and epigenetic modification of Debao porcine somatic cell nuclear transfer (SCNT) embryos. Treatment of oocytes with 1 μM MG132 from 30 h to 42 h of maturation and SCNT embryos with 5 μM MG132 for 2 h after fusion resulted in higher blastocyst yield (36.5%) of SCNT embryos compared with the control group (11.0%). The ZGA of SCNT embryos at 2- and 4-cell stages was also enhanced by MG132 treatment through altering the RNA pol II status and increasing the expression of eIF3A and TFIIA...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407859/establishment-of-intraperitoneal-germ-cell-transplantation-for-critically-endangered-chinese-sturgeon-acipenser-sinensis
#9
Huan Ye, Chuang-Ju Li, Hua-Mei Yue, Hao Du, Xiao-Ge Yang, Tasuku Yoshino, Takao Hayashida, Yutaka Takeuchi, Qi-Wei Wei
Recent progress in germ cell transplantation techniques in fish has paved the way for the conservation of endangered species. Here, we developed an intraperitoneal germ cell transplantation procedure using Chinese and Dabry's sturgeon as donor and recipient species, respectively. Histological analysis revealed that primordial germ cells migrated on the peritoneal wall at 16 days post-hatch (dph) in Dabry's sturgeon. The genital ridges of Dabry's sturgeon (recipient) first formed at 28 dph, suggesting that for successful colonization of donor germ cells in the recipient gonads, the transplantation should be performed earlier than this age...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407858/influence-of-lipoproteins-at-dry-off-on-metabolism-of-dairy-cows-during-transition-period-and-on-postpartum-reproductive-outcomes
#10
Martina Crociati, Lakamy Sylla, Claudia Floridi, Antonella Comin, Gabriele Fruganti, Maurizio Monaci, Giuseppe Stradaioli
High-yielding dairy cows are metabolically challenged during transition, when intense mobilization and hepatic oxidation of lipids is achieved, thus leading to fatty infiltration, ketosis and generalized inflammation. The condition is associated to periparturient diseases and poor fertility. The aim of this study was to assess whether serum lipoprotein concentrations in the dry period could influence the occurrence of postpartum diseases and reproductive performance in dairy cows. The study was carried out on 30 multiparous Holstein Friesian cows...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407857/a-review-of-the-ongoing-discussion-about-definition-diagnosis-and-pathomechanism-of-subclinical-endometritis-in-dairy-cows
#11
REVIEW
Karen Wagener, Christoph Gabler, Marc Drillich
In the last decade, several new aspects of the inflammation of the bovine endometrium have been investigated and described, including a new definition of subclinical endometritis. This review summarizes the recent discussion about the definition, diagnosis and pathomechanism of subclinical endometritis. Subclinical endometritis also referred to as cytological endometritis is defined by findings of endometrial cytology, which is usually performed with the cytobrush-technique or by low-volume flushing of the uterus...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407856/relationship-between-circulating-progesterone-at-timed-ai-and-fertility-in-dairy-cows-subjected-to-gnrh-based-protocols
#12
M G Colazo, I López Helguera, A Behrouzi, D J Ambrose, R J Mapletoft
The objectives of this retrospective study were: 1) to investigate the effect of plasma progesterone (P4) concentrations at the time of timed-AI (TAI) on fertility, and 2) to examine risk factors associated with plasma P4 concentrations that impair fertility in lactating dairy cows subjected to GnRH-based protocols. Data from 872 lactating Holstein cows that had, or had not been presynchronized prior to a 7-day GnRH-based TAI protocol were examined. However, data from only those cows (n = 697; 79.9%) that ovulated after second GnRH were analyzed...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407855/corrigendum-to-reducing-treatments-in-cattle-superovulation-protocols-by-combining-a-pituitary-extract-with-a-5-hyaluronan-solution-is-it-able-to-diminish-activation-of-the-hypothalamic-pituitary-adrenal-axis-compared-to-the-traditional-protocol-theriogenology
#13
Andrea Biancucci, Tatiana Sbaragli, Antonella Comin, Lakamy Sylla, Maurizio Monaci, Tanja Peric, Giuseppe Stradaioli
No abstract text is available yet for this article.
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407854/corrigendum-to-failure-of-a-single-dose-of-medroxyprogesterone-acetate-to-induce-uterine-infertility-in-postnatally-treated-domestic-cats-theriogenology-85-4-2016-718-723
#14
M Lopez Merlo, M Faya, P G Blanco, A Carransa, C Barbeito, C Gobello
No abstract text is available yet for this article.
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407853/effects-of-environmental-enrichment-on-reproductive-performance-and-quantity-and-morphology-of-cumulus-oocyte-complexes-obtained-from-rattus-norvegicus
#15
Joana Fisch, Iáskara Vieira de Oliveira, Juliana Fank, Lia Mara Gomes Paim, Marília Remuzzi Zandoná, Eliana Franco Lopes, Fernanda Bastos de Mello, Alexandre Tavares Duarte de Oliveira
Several researchers have observed that environmental enrichment (EE) can be effective in reducing stressful conditions and abnormal behavior and may provide better reproductive performance in rodents. In this context, this study aimed to evaluate the reproductive performance of Wistar rats reared in three different housing conditions. Animals were separated into breeding pairs, one pair per cage and pairs randomly assigned to three experimental groups (ten couples per group): control group were provided cages without any environmental enrichment; PVC group with PVC pipe; and cardboard roll group with a commercially available cardboard tube...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407852/establishment-and-characterization-of-a-telomerase-immortalized-porcine-luteal-cells
#16
Liang Zhang, Yong Huang, Zhenyu Wang, Xiaomao Luo, Hongling Zhang, Qian Du, Lingling Chang, Xiaomin Zhao, Dewen Tong
Luteal cells play a crucial role in pregnancy through secreting progesterone to maintain pregnancy and support of fetus. However, low cellular yields and inability to passage primary porcine luteal cells (PLCs) in vitro limit the luteal cell study. Therefore, developing an immortalized porcine luteal cell line is necessary for studying luteal cells activity and function in different diseases. In this study, primary PLCs were obtained from gilts at day 30 to day 50 of gestation and immortalized by human telomerase reverse transcriptase (hTERT)...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407851/time-dependent-changes-and-prognostic-value-of-lactatemia-during-the-first-24%C3%A2-h-of-life-in-brachycephalic-newborn-dogs
#17
C Castagnetti, M Cunto, C Bini, J Mariella, S Capolongo, D Zambelli
Blood lactate concentration is known to be a good prognostic indicator associated with the severity of illness and the patient's outcome both in human and veterinary medicine. It also plays a significant role in the assessment of the newborn, being a good indicator of fetal hypoxia and the ideal predictor of morbidity at term in babies. In veterinary neonatal medicine, hyperlactatemia is considered a valid prognostic marker in critically ill foals; moreover, blood lactate measurement has been proposed for the evaluation of newborn viability and the assessment of fetal distress during delivery in dogs...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407850/association-between-subclinical-hypocalcemia-in-the-first-3-days-of-lactation-and-reproductive-performance-of-dairy-cows
#18
L S Caixeta, P A Ospina, M B Capel, D V Nydam
The objective of this study was to determine the effects of subclinical hypocalcemia on reproductive performance in dairy cows. In a prospective cohort study, 97 cows on 2 dairy farms with automatic milking systems were monitored for subclinical hypocalcemia. Animals were enrolled 7 ± 3 days prior to estimated calving date and three parity groups were defined based on the lactation that the animals were going to start: lactation = 1, lactation = 2, and lactation ≥3. Serum calcium concentration (Ca) was measured in all animals in the first 3 DIM and subclinical hypocalcemia (SCH) was defined as Ca ≤ 8...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28257874/elevated-non-esterified-fatty-acid-and-%C3%AE-hydroxybutyrate-in-transition-dairy-cows-and-their-association-with-reproductive-performance-and-disorders-a-meta-analysis
#19
Amine Abdelli, Didier Raboisson, Rachid Kaidi, Belabdi Ibrahim, Amer Kalem, Mokrane Iguer-Ouada
A meta-analysis including 36 different results of statistic models from 14 papers was conducted. It evaluated the association between elevated non-esterified fatty acids and/or β-hydroxybutyrate (BHB) on the reproduction outcomes that were pregnancy at first insemination, estrous cyclicity, time to pregnancy, metritis and placental retention. Each association between BHB or NEFA and an outcome reported in literature was a model considered as raw-data for the meta-regression. For each outcome, the meta-regression adjusted the odds ratio, relative risk or hazard ratio with various moderators to reduce the heterogeneity among the studies...
April 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28257873/bacterial-invasion-of-the-uterus-and-oviducts-in-bovine-pyometra
#20
C C Karstrup, H G Pedersen, T K Jensen, J S Agerholm
Pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. Bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. Bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by applying fluorescence in situ hybridization using probes targeting 16S ribosomal RNA of Fusobacterium necrophorum, Porphyromonas levii, Trueperella pyogenes and the overall bacterial domain Bacteria...
April 15, 2017: Theriogenology
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