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Theriogenology

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https://www.readbyqxmd.com/read/28930668/aspects-of-sexual-precocity-and-morphometry-of-uterus-placenta-and-embryos-fetuses-in-piau-breed-and-commercial-line-gilts
#1
José Carlos Montes, Jurandy Mauro Penitente-Filho, Simone Eliza Facioni Guimarães, Paulo Sávio Lopes, Breno Soares Camilo, Hugo Hideki Shiomi, Daniel Araújo Lima, Rogério Oliveira Pinho, Jhonata Vieira Tavares do Nascimento Pereira, Denise Silva Okano, Karine Assis Costa, José Domingos Guimarães
In view of the importance of the genetic material of local breeds in the swine industry and the lack of information about reproductive performance of Piau females, two experiments were conducted to evaluate puberty and sexual maturity as well as the morphometry of embryos/fetuses, placenta and uterus during the first 90 days of gestation in Piau breed and Commercial line gilts. In experiment I, 37 Piau and 25 commercial line gilts were used. From the 120 days of age, detection of estrus was performed using mature boars from the first to third estrus of each gilt...
September 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/28916209/towards-new-strategies-to-manage-livestock-reproduction-using-kisspeptin-analogs
#2
M Beltramo, C Decourt
The discovery of the hypothalamic neuropeptide kisspeptin and its receptor (KISS1R) have dramatically improved our knowledge about the central mechanisms controlling reproduction. Kisspeptin neurons could be considered the hub where internal and external information controlling reproduction converge. The information is here elaborated and the command dispatched to GnRH neurons, the final output of the brain system controlling reproduction. Several studies have shown that in mammals administration of kisspeptin could finely modulate many aspects of reproduction from puberty to ovulation...
September 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923704/cloning-characterisation-docking-and-expression-analysis-of-3-beta-hydroxysteroid-dehydrogenase-during-ontogenetic-development-and-annual-reproductive-cycles-in-catfish-clarias-batrachus
#3
Irfan Ahmad Bhat, Mohd Ashraf Rather, Mir Ishfaq Nazir, Gireesh-Babu Pathakota, Mukunda Goswami, J K Sundaray, Rupam Sharma
Fish like higher animals, have a well-defined mechanism to produce sex steroids that play a critical role in gonadal development and maturation. In this study, we aimed to analyse the expression pattern of 3β-HSD in different tissues, during ontogenetic development and gonadal recrudescence of Clarias batrachus. A full-length cDNA of 1617 bp including an open reading frame (ORF) of 1125 bp encoding 374 amino acids was isolated from testes of C. batrachus. The docking analysis between C. batrachus 3β-HSD protein and eurycomanone exhibited high binding affinity toward each other with total energy of -108...
September 11, 2017: Theriogenology
https://www.readbyqxmd.com/read/28911882/aromatase-inhibitors-a-new-approach-for-controlling-ovarian-function-in-cattle
#4
M J Yapura, E M Zwiefelhofer, R A Pierson, G P Adams
Numerous treatments and protocols have been used to control the reproductive cycle in cattle, with varying effectiveness and many involving the administration of steroid hormones. Steroid hormones, such as estradiol, are perceived as having a negative impact on consumer health. This internationally shared opinion has led to a ban on the use of steroid hormones in food producing animals in many countries (i.e., European Union, New Zealand, and Australia). Letrozole, a non-steroidal aromatase inhibitor, inactivates the aromatase enzyme responsible for the synthesis of estrogens by reversibly binding to the "heme" group of the P450 subunit...
September 11, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923707/insemination-extender-supplementation-with-bestatin-and-edta-has-no-effect-on-rabbit-reproductive-performance
#5
L Casares-Crespo, P Fernández-Serrano, J S Vicente, E Mocé, C Castellini, A M Stabile, M P Viudes-de-Castro
The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, β nerve growth factor (β-NGF) degradation and reproductive performance after artificial insemination...
September 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923705/effect-of-gnrh-on-ovulatory-response-after-luteolysis-induced-by-two-low-doses-of-pgf2%C3%AE-in-lactating-dairy-cows
#6
Tsung-Ching Liu, Chen-Fei Chiang, Chia-Tang Ho, Jacky Peng-Wen Chan
This study investigated the effect of gonadotropin-releasing hormone (GnRH) on ovulatory response after complete luteolysis induced by two low doses of prostaglandin (PG)F2α in lactating dairy cows. Cows (n = 18) ranging between 45 and 65 days in milk were recruited for synchronization by a modified Ovsynch-48 protocol (GnRH-7 days-375 μg PGF2α-1 day-250 μg of PGF2α-1 day-GnRH) over a total of 23 estrous cycles. Synchronized cows (n = 16) were randomly assigned to GnRH and Saline groups in stage 1 of the experiment after 9-10 days of ovulation in synchronization...
September 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923703/transcriptome-analysis-of-three-critical-periods-of-ovarian-development-in-yellow-river-carp-cyprinus-carpio
#7
Yongfang Jia, Ping Nan, Wanwan Zhang, Fang Wang, Ruihua Zhang, Tingting Liang, Xiaolin Ji, Qiyan Du, Zhongjie Chang
Ovary development is a complex process involving numerous genes; the molecular mechanism underlying the ovary development of carp is still unknown. Here we used Illumina HiSeq™ 2500 to explore the transcriptome of undifferentiated gland (PG), juvenile ovary (OJ) and adult ovary (OA) of Yellow River carp (Cyprinus carpio). A total of 58,749 unigenes were obtained, comprising 45,707 known genes and 13,042 new genes. We identified differentially-expressed genes (DEGs) during development and characterized the functional properties of DEGs by comparison with the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes databases...
September 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923706/specific-interferon-tau-gene-regulation-networks-in-bovine-endometrial-luminal-epithelial-cells
#8
Gan Zhao, Kangfeng Jiang, Tao Zhang, Haichong Wu, Changwei Qiu, Ganzhen Deng
Interferon tau (IFNT) plays a critical role as a pregnancy recognition factor in early pregnancy by regulating uterine epithelial gene expression. Illuminating the relation between IFNT and pregnancy will contribute significantly to early pregnancy research in ruminants. Therefore, in this study, we treated primary bovine endometrial luminal epithelial cells (bELECs) without or with IFNT (200 ng/mL) for 6 or12 h. Subsequently, RNA sequencing (RNA-seq) technology was used to evaluate differences in gene expression...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28917726/ovulation-and-fertility-responses-for-sows-receiving-once-daily-boar-exposure-after-weaning-and-ovugel-%C3%A2-followed-by-a-single-fixed-time-post-cervical-artificial-insemination
#9
R R Ulguim, F P Bortolozzo, I Wentz, M Johnston, S K Webel, L Arend, R V Knox
Boar exposure is used to stimulate follicle development and estrus in sows after weaning and also to improve semen uptake and sperm transport with insemination. However, the need and value of boar exposure is uncertain when ovulation induction is used. These studies were designed to determine the effect of daily boar exposure after weaning when used with ovulation induction and fixed time post-cervical artificial insemination (PCAI). In experiment 1, sows were weaned into stalls and assigned to receive 3 min of daily fenceline boar exposure (BE, n = 7) or no boar exposure (NBE, n = 8)...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28917127/differential-expression-of-ten-candidate-genes-regulating-prostaglandin-action-in-reproductive-tissues-of-buffalo-during-estrous-cycle-and-pregnancy
#10
Ankita Dillipkumar Verma, Manjit Panigrahi, Naseer Ahmad Baba, Sourabh Sulabh, Abdul Sadam, Subhashree Parida, Krishnaswamy Narayanan, Arvind A Sonwane, Bharat Bhushan
Prostaglandins (PGs) are the key mediators of several female reproductive functions, including luteolysis, ovulation, fertilization, implantation, pregnancy, and parturition. The present study was conducted in buffalo endometrial and luteal tissues between nonpregnant and two stages of pregnancy (29-38 days of pregnancy, 48-56 days of pregnancy) tissue samples. The genes involved from synthesis upto receptor level effect of PGs (PGF2α and PGE2) were studied for their relative mRNA expression. We have collected the endometrial and luteal tissues from slaughtered animals and confirmed the stages by external examination and crown vertebral rump length measurement of the foetus...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/28917126/effects-of-a-single-administration-of-different-gonadotropins-on-day-7%C3%A2-post-insemination-on-pregnancy-outcomes-of-rabbit-does
#11
N M Hashem, Z R Aboul-Ezz
This study aimed to investigate the effects of a single administration of one of three different gonadotropins on Day 7 post-insemination on ovarian activity, progesterone (P4) concentration and pregnancy outcomes of rabbit does. Multiparous, non-lactating, V-line does were artificially inseminated after synchronization and ovulation induction with equine chorionic gonadotropin (eCG; 25 IU im) and gonadotropin releasing hormone (GnRH; 0.8  μg buserelin im) 48 h later. On Day 7 post-inseminarion, does were randomly allocated into four groups (n = 40/group)...
September 7, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923708/enhanced-in%C3%A2-vitro-maturation-of-canine-oocytes-by-oviduct-epithelial-cell-co-culture
#12
Jingu No, Minghui Zhao, Seunghoon Lee, Sun A Ock, Yoonseok Nam, Tai-Young Hur
Canine-assisted reproductive techniques have been successful for several years; however, the lack of an oocyte in vitro maturation system has limited their application. The aim of this study was to evaluate the effect of canine oviduct epithelial cells (cOECs) on canine oocyte maturation in vitro. Specifically, the method used for isolation of cOECs did not affect the expression of epithelial markers, E-cadherin and cytokeratin, on fresh, cultured and cryopreserved cells. Moreover, BrdU analysis showed that cOECs cultured in Medium 171 supplemented with mammary epithelial growth supplement were more proliferative than counterparts in advanced Dulbecco's modified Eagle medium or Medium 199...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28888334/preparation-characterization-and-application-of-long-acting-fsh-analogs-for-assisted-reproduction
#13
David Ben-Menahem
Assisted reproduction technologies are widely used in humans and domestic animals and often include follicle stimulating hormone (FSH) in the protocol. One limitation with most of the available FSH preparations is the relative short half-life in the circulation that dictates multiple daily injections for the desired follicle development and superovulation. The development of bioactive long-acting structurally modified FSH analogs is desirable for human and veterinary use. In addition, optimal preparations and/or formulations are expected to improve the regimen and efficiency of the treatment...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28881231/follicles-and-gonadotropins-during-waves-2-and-3-in-three-wave-interovulatory-intervals-in-bos-taurus-heifers
#14
O J Ginther, M A R Siddiqui, E R Araujo, S V Dangudubiyyam
Observations were made on follicle dynamics and gonadotropin concentrations in anovulatory wave 2 and ovulatory wave 3 in three-wave interovulatory intervals (n = 15). Hypotheses were not used owing to inadequate availability of rationale. The future dominant follicles for waves 2 and 3 were designated DF2 and DF3 and the largest future subordinate follicles as SF2 and SF3, respectively. The day of expected diameter deviation (day 0) was defined as the day that DF2 or DF3 was closest to 8.5 mm. The first day that DF2 became smaller (P < 0...
August 30, 2017: Theriogenology
https://www.readbyqxmd.com/read/28881230/investigation-of-the-5-flanking-region-and-exon-3-polymorphisms-of-igf-1-gene-showed-moderate-association-with-semen-quality-in-sanjabi-breed-rams
#15
R Bakhtiar, A Abdolmohammadi, H Hajarian, Z Nikousefat, D Kalantar-Neyestanaki
In this study, semen samples were collected from 96 Sanjabi rams in order to investigate the IGF-1 gene polymorphisms and their relationship with the characteristics of semen quality and testicular size. The dimensions of scrotal length, width and circumference were measured during autumn and spring over two years. Blood samples were simultaneously collected from jugular vein to extract DNA. PCR was performed using specific primers to amplify 294 and 272bp fragments including 5' regulatory region and exon 3 of IGF-1 gene, respectively...
August 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28865297/effects-of-hcg-and-salmon-gonadoliberine-analogue-on-spermiation-in-the-eurasian-perch-perca-fluviatilis
#16
Daniel Żarski, Gergely Bernáth, Jarosław Król, Beata I Cejko, Zoltán Bokor, Katarzyna Palińska-Żarska, Sylvain Milla, Pascal Fontaine, Sławomir Krejszeff
This study analysed (i) the effect of human chorionic gonadotropin (hCG) and salmon gonadoliberine analogue (sGnRHa) on the effectiveness of induction of spermiation and (ii) the effect of latency time following the application of those spawning agents on the quantity and quality of the sperm of Eurasian perch, Perca fluviatilis, obtained during out-of-season spawning. For this study, pond-reared fish were used which had been acclimated to the controlled conditions. Three groups were distinguished which were treated with either saline (0...
August 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28863349/effect-of-bovine-somatotropin-injection-in-late-pregnant-holstein-heifers-on-metabolic-parameters-and-steroidogenic-potential-of-the-first-postpartum-dominant-follicle
#17
Diego Andres Velasco Acosta, Augusto Schneider, Carolina Bespalhok Jacometo, Joao Alvarado Rincon, Felipe Cardoso, Marcio Nunes Corrêa
The aim of this study was to determine the effect of pre-partum injections of bovine somatotropin (bST) in dairy heifers on metabolic markers and the steroidogenic potential of the first postpartum dominant follicle. Heifers were assigned to two groups: bST (ST; n = 29), that received two doses of bST (500 mg/dose) at -28 and -14 days relative to calving; and control (CTL; n = 30), that did not received bST. Follicular development was monitored via ultrasound every 3 days starting at 8 days in milk (DIM) in a subset of 20 heifers until the day the first large follicle reached a diameter of 16 mm...
August 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28863964/design-and-biological-effects-of-a-vaginally-administered-gel-containing-the-gnrh-agonist-triptorelin-for-synchronizing-ovulation-in-swine
#18
R V Knox, K R Stewart, W L Flowers, M E Swanson, S K Webel, R R Kraeling
A novel gel formulation was selected for intravaginal delivery of the GnRH agonist (triptorelin) for synchronizing ovulation in pigs. Studies with gilt models were used to assess LH response profiles. The lowest dose of triptorelin that induced the most gilts to show an LH surge was 100 μg in 1.2% methylcellulose gel. This formulation had a similar effect in weaned sows while also advancing ovulation. The timing of administration was evaluated in sows after weaning. Administration at 96 h induced more sows to ovulate (58%) by 48 h compared to treatment at estrus (45%) or for controls (34%), but the desired level of ovulation synchrony was not achieved...
August 24, 2017: Theriogenology
https://www.readbyqxmd.com/read/28888896/hormonal-profile-and-follicular-dynamics-concurrent-with-cidr-and-insulin-modified-ovsync-tai-programs-and-their-impacts-on-the-fertility-response-in-buffaloes
#19
A A Ramoun, A M Emara, B A Heleil, S A Darweish, H A Abou-Ghait
Fifty one cyclic Egyptian buffaloes were used to study the hormonal profile and follicular dynamics concurrent with CIDR and insulin modified Ovsync TAI programs and their impacts on the consequent fertility responses. The buffaloes were randomly assigned into 3 ovulation synchronization protocols: Ovsync-alone (n = 13, control) CIDR-sync (n = 20) and Insulin-sync (n = 18). Ovsync-alone protocol consisted of two im injections of 20 μg bueserlin (GnRHa) on Day 0 (GnRH 1) and on Day 9 (GnRH 2) with an im injection of 500 μg of cloprostenol sodium (PGF2 α) on Day 7...
August 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28846912/impact-of-estradiol-cypionate-prior-to-tai-and-progesterone-supplementation-at-initial-diestrus-on-ovarian-and-fertility-responses-in-beef-cows
#20
T Martins, J P Talamoni, M Sponchiado, J R G Maio, G P Nogueira, G Pugliesi, M Binelli
In cattle, early diestrus progesterone (P4) supplementation modulates endometrial function to exert pro- and anti-pregnancy establishment effects; specifically, P4 stimulates conceptus growth, but also induces early onset of luteolysis. This paradoxical effect is frequently related to the inconsistent fertility outcomes that result from P4 supplementation experiments. Aim was to investigate the impact of exogenous estradiol (E2) treatment at the end of timed fixed AI (TAI) on frequency of early luteolysis and pregnancy of beef cows supplemented with P4...
August 19, 2017: Theriogenology
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