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Amine Abdelli, Didier Raboisson, Rachid Kaidi, Belabdi Ibrahim, Amer Kalem, Mokrane Iguer-Ouada
A meta-analysis including 36 different results of statistic models from 14 papers was conducted. It evaluated the association between elevated non-esterified fatty acids and/or β-hydroxybutyrate (BHB) on the reproduction outcomes that were pregnancy at first insemination, estrous cyclicity, time to pregnancy, metritis and placental retention. Each association between BHB or NEFA and an outcome reported in literature was a model considered as raw-data for the meta-regression. For each outcome, the meta-regression adjusted the odds ratio, relative risk or hazard ratio with various moderators to reduce the heterogeneity among the studies...
April 15, 2017: Theriogenology
C C Karstrup, H G Pedersen, T K Jensen, J S Agerholm
Pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. Bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. Bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by applying fluorescence in situ hybridization using probes targeting 16S ribosomal RNA of Fusobacterium necrophorum, Porphyromonas levii, Trueperella pyogenes and the overall bacterial domain Bacteria...
April 15, 2017: Theriogenology
V S Machado, R C Neves, F S Lima, R C Bicalho
During the Presynch-Ovsynch protocol, at least half of the cows enrolled display signs of estrus, which can present as an opportunity for cows to be inseminated before the completion of the protocol. The primary objective of this study is to compare two management strategies for first service using the Presynch-Ovsynch protocol: insemination at completion of the Presynch-Ovsynch program (TAIonly) or insemination after estrus detection during the Presynch-Ovsynch protocol, with the remainder of cows being inseminated at timed artificial insemination (ED + TAI)...
April 15, 2017: Theriogenology
Ling Yang, Shengqin Zang, Ying Bai, Xiaolei Yao, Leying Zhang
Lymph nodes are the sites where the immune reaction or suppression takes place. Progesterone (P4) exerts an essential effect of the immunomodulation on the maternal uterus during early pregnancy in ruminants. At present study, the inguinal lymph nodes were obtained at day 16 of non-pregnancy, days 13, 16 and 25 of pregnancy (n = 3 for each group) in ewes, and RT-PCR assay, western blot and immunohistochemistry analysis were used to analyze to the effect of early pregnancy on the expression of P4 receptor (PGR) and progesterone-induced blocking factor (PIBF) in the lymph nodes...
April 15, 2017: Theriogenology
Monica De Los Reyes, Jaime Palomino, Victor H Parraguez, Fernando Ramirez
The aim of this study was to determine the mRNA LHR and LHR protein expression pattern in the canine ovarian follicles at different stage of development throughout the estrous cycle. Dog ovaries were obtained from 1-6y bitches at proestrus/estrus, anestrus and diestrus stages following ovariohysterectomy. Follicular cells were mechanically recovered from follicles distributed into four types (preantral, small antral, medium antral and large antral). Total RNA extraction was performed and the evaluation of gene expression levels was achieved by relative quantification q-PCR analysis...
April 15, 2017: Theriogenology
Kaitlynn M Abell, Miles E Theurer, Robert L Larson, Brad J White, David K Hardin, Richard F Randle, Robert A Cushman
The objective of this project was to quantify patterns in the calving rate of sires in multiple-sire pastures over seven years at a large-scale cow-calf operation. Data consisted of reproductive and genomic records from multiple-sire breeding pastures (n = 33) at the United States Meat Animal Research Center (USMARC) from 2007 to 2013. Calving intervals were analyzed in 21-day periods. A ranking system for each bull was developed based on the calving rate per pasture over the breeding season, with Rank 1 = the bull with greatest calving rate, Rank 3 = the bull with the least calving rate, and Rank 2 = all other bulls...
April 15, 2017: Theriogenology
Natalia Gabriela Canel, Romina Jimena Bevacqua, María Inés Hiriart, Natana Chaves Rabelo, Luiz Sergio de Almeida Camargo, Marina Romanato, Lucrecia Piñeiro de Calvo, Daniel Felipe Salamone
In bovine, intracytoplasmic sperm injection (ICSI) remains inefficient partially due to low levels of sperm decondensation. The aim of this study was to determine whether the injection of normal size sperm pretreated with heparin (Hep) and l-glutathione (GSH), the use of sex-sorted sperm, the double round of sperm freezing/thawing (re frozen), or the combination of these approaches can improve sperm decondensation and embryo development after ICSI in cattle. Cleavage and blastocyst rates were evaluated on days 2 and 7 post ICSI...
April 15, 2017: Theriogenology
Federica Zacchini, Paola Toschi, Grazyna Ewa Ptak
Pregnancies obtained by Assisted Reproductive Technologies are at higher risk of miscarriage than those obtained naturally. Previously, we reported impaired placental vascular development of in vitro produced (IVP) sheep embryos and defective DNA methylation in the placentae of those embryos. One reason behind these observed defects may be an impaired One Carbon Metabolism (OCM) The present study was performed to test the hypothesis that Cobalamin (Vitamin B12, an important OCM co-factor) supplementation during IVM corrects DNA methylation of IVP embryos and, consequently, ameliorates placental vasculogenesis...
April 15, 2017: Theriogenology
O López Albors, F Olsson, A B Llinares, H Gutiérrez, R Latorre, E Candanosa, A Guillén-Martínez, M J Izquierdo-Rico
The expression and localization of VEGFA and its main receptors Flt-1 and KDR is characterized in the oviduct throughout the porcine estrous cycle. Oviducts were sampled from sows at early follicular, late follicular, early luteal and late luteal stages of the estrous cycle and a segment from the mid portion of the ampulla and isthmus studied by real time RT-PCR and quantitative immunohistochemistry. The expression of the three components of the VEGF system was continuous, although differences were observed depending on the oviduct portion, the stage of the estrous cycle and the histological layer...
April 15, 2017: Theriogenology
Mehmet Salih Kaya, Mehmet Kose, Aydin Guzeloglu, Zekeriya Kıyma, Mehmet Osman Atli
In the present study, we aimed to 1) demonstrate the presence of all 10 toll-like receptors (TLRs) in ovine trophoblasts, and 2) investigate the expression profiles of TLR1-10 mRNAs in peripheral blood leukocytes (PBLs) in ewes during early pregnancy. For those purposes, ovine trophoblasts (n = 6) were collected from pregnant ewes on day 13. PBLs were collected from non-pregnant (n = 6) and pregnant ewes (n = 17) on days of mating (d) 0 and 18. TLR mRNAs in ovine trophoblasts were visualized by free-floating in situ hybridization (ISH)...
April 15, 2017: Theriogenology
B S Holst, E Holmroos, L Friling, S Hanås, L-M Langborg, M A Franko, K Hansson
Benign prostatic hyperplasia (BPH) is an age-related disorder in the intact male dog that is associated with an increase in the prostatic size. Ultrasonography gives a reliable estimate of the prostatic size, but a method for screening the prostate size using a serum sample has advantages, such as requiring less expensive equipment. The primary aim of the study was to study the association between the concentration of the circulating biomarker canine prostate specific esterase (CPSE) and prostatic size. Seventy-nine dogs that were four years old or older were included in the study...
April 15, 2017: Theriogenology
Lei Shi, Ruigao Song, Xiaolei Yao, Youshe Ren
The objective of this study was to investigate the effects of selenium (Se) on in vitro proliferation, apoptosis and testosterone production of sheep Leydig cells and its underlying mechanism. Leydig cells were collected from 8-month-old sheep and divided into four treatment groups (0, 2.0, 4.0 and 8.0 μmol/L Se). After treatment with Se for 48 h, the MTT and flow cytometric assay were used to detect cell proliferation and apoptosis. Testosterone level in the culture medium was determined by ELISA. The mRNA expression and protein abundance of cell cycle, apoptosis and testosterone synthesis-related genes were detected using real-time PCR and western blot analysis...
April 15, 2017: Theriogenology
Zexing Wen, Yangyang Pan, Yan Cui, Xiumei Peng, Ping Chen, Jiangfeng Fan, Guyue Li, Tian Zhao, Jian Zhang, Shujian Qin, Sijiu Yu
Colony-stimulating factor 2 (CSF2) is known to promote the development and survival of rodents and ruminants preimplantation embryos; however, the effect of CSF2 on yak embryos has not been reported. The objective of this study was to investigate the effects of CSF2 on the developmental competence of yak embryos cultured in vitro in modified synthetic oviduct fluid (mSOF) medium and on the expression pattern of heat shock protein 70 kDa 1A (HSPA1A). In each experiment, cumulus-oocyte complexes (COCs) were matured in vitro and fertilized with frozen-thawed semen...
April 15, 2017: Theriogenology
Nur Siti Khadijah Ramli, Nelli Giribabu, Sekaran Muniandy, Naguib Salleh
No abstract text is available yet for this article.
April 15, 2017: Theriogenology
J B Roelofs, C Krijnen, E van Erp-van der Kooij
When the daily routine of a cow is disturbed, it may have a detrimental effect on the performance of activity meters to detect estrus. It is possible that during the pasture period, the daily routine of cows is disturbed, adversely affecting the performance of activity meters to detect estrus which does not happen when the cows are housed indoors. The objective of this study was to investigate whether housing conditions (pasture or indoor) affected the performance of activity meters to detect estrus in dairy cows...
April 15, 2017: Theriogenology
Léonie Morin-Doré, Patrick Blondin, Christian Vigneault, François-Xavier Grand, Rémi Labrecque, Marc-André Sirard
Assisted reproduction technologies (ART) and high selection pressure in the dairy industry are leading towards the use of younger females for reproduction, thereby reducing the interval between generations. This situation may have a negative impact on embryo quality, thus reducing the success rate of the procedures. This study aimed to document the effects of oocyte donor age on embryo quality, at the transcriptomic level, in order to characterize the effects of using young females for reproduction purpose...
April 15, 2017: Theriogenology
Chao Wang, Jia-Lian Li, Hong-Kui Wei, Yuan-Fei Zhou, Si-Wen Jiang, Jian Peng
This study aimed to investigate the factors influencing the boar herd life month (BHLM) in Southern China. A total of 1630 records of culling boars from nine artificial insemination centers were collected from January 2013 to May 2016. A logistic regression model and two linear models were used to analyze the effects of breed, housing type, age at herd entry, and seed stock herd on boar removal reason and BHLM, respectively. Boar breed and the age at herd entry had significant effects on the removal reasons (P < 0...
April 15, 2017: Theriogenology
Ding-Ping Bai, Ming-Ming Yang, Lei Qu, Yu-Lin Chen
The development of transgenic technologies in the Cashmere goat (Capra hircus) has the potential to improve the quality of the meat and wool. The piggyBac (PB) transposon system is highly efficient and can be used to transpose specific target genes into the genome. Here, we developed a PB transposon system to produce transgenic Cashmere goat fetal fibroblasts (GFFs) with the enhanced green fluorescent protein (EGFP). We then used the genetically modified GFFs as nuclear donors to generate transgenic embryos by somatic cell nuclear transfer (SCNT)...
April 15, 2017: Theriogenology
Agnieszka Mostek, Mariola Aleksandra Dietrich, Mariola Słowińska, Andrzej Ciereszko
Artificial insemination with cryopreserved semen enables affordable, large-scale dissemination of gametes with superior genetics. However, cryopreservation can cause functional and structural damage to spermatozoa that is associated with reactive oxygen species (ROS) production, impairment of sperm motility and decreased fertilizing potential, but little attention has been paid to protein changes. The goal of this study was to investigate the oxidative modifications (measured as carbonylation level changes) of bull spermatozoa proteins triggered by the cryopreservation process...
April 1, 2017: Theriogenology
M Gobikrushanth, P A Dutra, T C Bruinjé, M G Colazo, S T Butler, D J Ambrose
The objectives were to determine (1) the repeatability of antral follicle counts (AFC) and anti-Müllerian hormone (AMH) concentrations at an unknown stage of follicular growth (FG) and at an expected day of follicular wave emergence (FWE) in dairy cows, and (2) the association between AFC and AMH at the above two stages. Transrectal ultrasound imaging of the ovaries and blood sample collection were performed on 91 lactating Holstein cows (33 primiparous, 58 multiparous) to determine AFC and serum concentrations of AMH at an unknown stage of FG (mean ± SEM; 14 ± 0...
April 1, 2017: Theriogenology
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