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Mayra H Frediani, Fernanda J V Guida, Paula A B Salgado, Débora R Gonçalves, Marcel H Blank, Gabriel A Novaes, Ricardo J G Pereira
It is unquestinable that artificial insemination (AI) offers many benefits to avian conservation programs, but a serious impediment towards implementing AI for wild species is the development of effective techniques to consistently collect good quality ejaculates. Thus, we aimed to examine the success rate of electro-stimulation (ES) in collecting semen from 49 unconditioned males from orders Piciformes, Strigiformes, Accipitriformes, Cathartiformes, Galiformes, Anseriformes and Psittaciformes at different times of the year...
November 3, 2018: Theriogenology
Maira N Corso, Lis S Marques, Luis F G Gracia, Rômulo B Rodrigues, Leonardo J G Barcellos, Danilo P Streit
The production of captive fish is only possible through artificial reproduction, but manipulation is a known stressor stimulus. Thus, the objective of the present study was to evaluate the effects of different eugenol concentrations (0, 30, 40, 50 and 60 mg/L) during reproductive management of Rhamdia quelen. Seventy-five mature male R. quelen were randomly distributed among the five treatments, and blood samples were collected at the time of semen collection to measure plasma cortisol. The following parameters were evaluated in the fresh semen samples: motility, motility duration, concentration and fertilization rate...
October 31, 2018: Theriogenology
C Munari, P Ponzio, A R Alkhawagah, A Schiavone, C Mugnai
On commercial farms, rabbit does are subjected to a reproductive rhythm that does not account for their welfare or physiology, leading to reduced longevity and consequently high annual replacement. The European Food Safety Authority (EFSA) recommends limited and infrequent use of hormone treatments, and suggests replacement with alternative methods that do not threaten animal welfare when possible. In the present study, we aimed to determine whether the GnRH analogue lecirelin acetate could be administered by inclusion in the seminal dose during insemination...
October 31, 2018: Theriogenology
Riccardo Orlandi, Emanuela Vallesi, Cristiano Boiti, Angela Polisca, Alessandro Troisi, Cecilia Righi, Paolo Bargellini
We evaluated the potential usefulness of CEUS to assess fetal-maternal circulation during pregnancy in dogs. Nine bitches were examined at 23, 30, and 45 days of gestation using an ultrasound machine (LOGIQ E9) and SonoVue® contrast media as echo-signal enhancer. Qualitative and quantitative evaluation of contrast enhancement patterns of uterine artery and utero/placental vessels were performed on recorded images. Independently of the gestational periods, the qualitative evaluation showed the initial wash-in phase from the first appearance of the uterine artery to the rapid distribution in embryonic vesicles or placenta to the progressive washout, whilst there was no enhancement of either embryos or fetuses in any bitch...
October 30, 2018: Theriogenology
Malgorzata Grzesiak, Ewa Waszkiewicz, Magdalena Wojtas, Kinga Kowalik, Anita Franczak
Vitamin D3 acting via its nuclear receptor (VDR) was shown to target many reproductive tissues and regulate their function. Nevertheless, little is known about the role of vitamin D3 and VDR in the uterus. We hypothesized that VDR expression profile varies in the porcine uterus throughout the course of the estrous cycle, and 1,25(OH)2 D3 influences uterine steroidogenic activity. The aim of this study was to investigate VDR mRNA expression, VDR protein abundance and immunolocalization in the porcine endometrium and myometrium harvested on Days 2-5, 12-13, 15-16 and 18-20 of the estrous cycle...
October 30, 2018: Theriogenology
Felipe Martínez-Pastor, Mercedes Álvarez, Camino Guerra, César A Chamorro, Luis Anel-López, Paulino de Paz, Luis Anel, Manuel Álvarez-Rodríguez
Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol)...
October 29, 2018: Theriogenology
B C Perez, J C C Balieiro, G A Oliveira Junior, L T Andrietta, R G Vizoná, R V Ventura, F A T Bruneli, M G C D Peixoto
The main objective of this study was to verify genetic trends for milk production (MY305) and age at first calving (AFC). Were also considered levels of inbreeding practiced in the Brazilian dual-purpose Guzerá population (TPOP ) comparing it with the same parameters estimated for two sub-populations derived from the reference (TPOP ): female donors submitted to ovum-pick up (DPOP ) and in vitro embryos produced (EPOP ) between 2003 and 2013. Estimated breeding values (EBV) and inbreeding coefficients (F) were regressed by the year of birth (or year of in vitro fertilization) of each animal or embryo in order to obtain annual trends for these parameters separately for each of the three populations studied...
October 29, 2018: Theriogenology
O J Ginther, S V Dangudubiyyam, R R Domingues
Selection of the dominant follicle during a follicular wave is manifested by diameter deviation. At deviation (day 0), growth rate continues for the future dominant follicle (F1) and begins to decrease for the largest subordinate follicle (F2). The percentage of color-Doppler blood-flow signals in the wall of F1 and F2 and the temporality between FSH concentration and F1 and F2 diameter were determined daily in waves 1 and 2 in 24 Holstein heifers. Diameter and blood flow were compared among classes of deviation: (1) conventional (F2 ≥ 7...
October 26, 2018: Theriogenology
Vanmathy Kasimanickam, Mary Buhr, Ramanathan Kasimanickam
Although sperm and seminal plasma differ in their origin, biophysical and biochemical properties of seminal plasma influence the sperm function. Seminal plasma is a fluid medium containing substances from testes, epididymides and accessory glands. Composition of seminal plasma varies among animal species and in boars, prostate and bulbourethral glands are major contributors to the volume and contents. While the origin of some components of seminal plasma are known, the source of recently discovered seminal plasma microRNAs remains unknown, in part due to the difficulty of recovering and characterizing RNA from porcine sperm and seminal plasma...
October 26, 2018: Theriogenology
Ana Paula Pinoti Pavaneli, Marina da Silva Passarelli, Flávia Vieira de Freitas, Gisele Mouro Ravagnani, Mariana Andrade Torres, Simone Maria Massami Kitamura Martins, Marc Yeste, André Furugen Cesar de Andrade
Seminal plasma (SP) plays a vital role in the maintenance of sperm function and integrity along with being involved in their communication with the female reproductive tract. Under in vitro conditions, although it is generally accepted that boar semen is better preserved when SP is diluted (extended) or removed (cryopreserved), its role during storage is not completely elucidated. In this context, the current study sought to determine the role of SP during storage of boar spermatozoa at 17 °C for 72 h...
October 25, 2018: Theriogenology
Fk Hollinshead, Dw Hanlon
Appropriate timing of insemination in the bitch, through monitoring of serial serum progesterone concentrations, has historically been based on the detection of important physiological reproductive events which include the LH surge and ovulation 2-3 days later. Previous hormone profile studies during the estrus period in the bitch, in which determination of these physiological reproductive events has been defined, have been based on low numbers of bitches of similar breeds and body weights. Therefore, the aim of this large-scale prospective study was to define the normal serum progesterone profile during the estrus period in a large number of bitches of various breeds and body weights...
October 22, 2018: Theriogenology
R M Kirberger, E G Bester, M L Schulman, I Janse van Rensburg, M J Hartman
The study objectives were to measure gestational sac (GS) diameter and crown-to-rump (CR) length in conscious pregnant rats and to determine the chronological ultrasonographic appearance of heart beat and fetal organogenesis. The study formed part of a unilateral surgical salpingectomy trial with 16 female Sprague-Dawley rats (Rattus norvegicus). Ten rats were operated on while the other six served as controls. After surgery all were mated at 8-10 weeks of age. Gestational length was taken as 22 days. Rats were manually restrained for abdominal ultrasonography and were scanned daily from day 7 until day 19 or 20 post-mating followed by immediate euthanasia...
October 21, 2018: Theriogenology
Xinyu Wang, Qing Yang, Ke Wang, Hailong Yan, Chuanying Pan, Hong Chen, Jinwang Liu, Haijing Zhu, Lei Qu, Xianyong Lan
Growth differentiation factor 9 (GDF9) gene is an effective intra-ovarian regulator; it plays a crucial role in early folliculogenesis in female mammals. The non synonymous mutations: g.3905A > C (also known as p.Gln320Pro/Q320P) and g.4135G > A (also know as p.Val397Ile/V397I), are two well-known and controversial single nucleotide polymorphisms (SNPs) within GDF9 gene in goats with different prolificacy, and so far, there were no studies on linkage between Q320P and V397I. Therefore, the aim of this work was to study whether Q320P and V397I mutations have a significant effect on litter size, in Shaanbei white cashmere goats (SBWC, n = 1511), and to explore the specific relationship between these two SNPs...
October 19, 2018: Theriogenology
G G Macedo, R D Mingoti, E O S Batista, B M Monteiro, L M Vieira, R V Barletta, M C Wiltbank, G P Nogueira, F P Rennó, J R Maio, P S Baruselli
The main objective of this study was to evaluate the effect of intramuscular (I.M.) administration of different doses of kisspeptin (Kp) on the pattern of luteinizing hormone (LH) release in Bos taurus and Bos indicus prepubertal heifers. Holstein heifers weighing 215.1 ± 38.6 kg (n = 24; aged 5-8 mo) and Gyr heifers weighing 215.4 ± 31.4 kg (n = 24; aged 6-10 mo) were enrolled in this study. The animals were confirmed as non-cyclical by absence of a corpus luteum (CL) as determined by ultrasound scanning of the ovaries and low circulating P4 concentrations (<1...
October 19, 2018: Theriogenology
S M Rowe, L Pryor, W P Tranter, J Hosie, J Cavalieri
This study evaluated the effect of equine chorionic gonadotropin (eCG) on reproductive performance, when incorporated into the first Ovsynch + P4 synchronization following planned start mating (PSM) in pasture-based lactating dairy cows. Two synchrony programs were compared in a randomized controlled trial in Queensland, Australia. Lactating cows from a single dairy herd (n = 782) were randomly allocated to Control and eCG groups. Control cows had their estrous cycles synchronized by treatment with 100 μg gonadotropin releasing hormone (GnRH; im) and insertion of a progesterone (P4) releasing intravaginal device that contained 1...
October 18, 2018: Theriogenology
Raphael Bermal Costa, Natalia Irano, Iara Del Pilar Solar Diaz, Luciana Takada, Isis da Costa Hermisdorff, Roberto Carvalheiro, Fernando Baldi, Henrique Nunes de Oliveira, Humberto Tonhati, Lucia Galvão de Albuquerque
The objective of this study was to assess the accuracy of genomic predictions for female reproductive traits in Nellore cattle. A total of 1853 genotyped cows and 305,348 SNPs were used for genomic selection analyses. GBLUP, BAYESCπ, and IBLASSO were applied to estimate SNP effects. The pseudo-phenotypes used as dependent variables were: observed phenotype (PHEN), adjusted phenotype (CPHEN), estimated breeding value (EBV), and deregressed estimated breeding value (DEBV). Predictive abilities were assessed by the average correlation between CPHEN and genomic estimated breeding value (GEBV) and by the average correlation between DEBV and GEBV in the validation population...
October 17, 2018: Theriogenology
M É S Bezerra, A P O Monte, R S Barberino, T L B G Lins, J L Oliveira Junior, J M S Santos, D O Bezerra, C A Neves, G C Silva, M A M Carvalho, M H T Matos
The aim of this study was to evaluate the effect of the conditioned medium of ovine Wharton's jelly-derived mesenchymal stem cells (oWJ-MSCs) on the morphology, growth, reactive oxygen species (ROS) and glutathione (GSH) intracellular levels, active mitochondria, and meiotic resumption of isolated ovine secondary follicles in vitro. The oWJ-MSCs were isolated and the medium where they were cultured was recovered (conditioned medium). Isolated ovine secondary follicles were cultured for 6 days in 1) supplemented α-MEM+ (control); 2) 50% α-MEM+  + 50% conditioned medium (α-MEM + CM group) or 3) conditioned medium only (CM group)...
October 13, 2018: Theriogenology
Felipe Urra, Marcelo H Ratto, Mauricio Silva
The aim of this study was to evaluate the endometrial vascularization area (EVA) of both uterine horns in llamas subjected to different intrauterine treatments resembling physiological conditions after a single mating. Llamas with a growing follicle (≥8 mm) were randomly assigned to: a) single mating with a fertile male (mating; positive control; n = 6); b) intramuscular administration of 50 μg of gonadorelin acetate plus an intrauterine infusion of 4 ml of PBS (GnRH; negative control; n = 4); c) intrauterine infusion of 4 ml of raw llama seminal plasma (SP; n = 4) or d) intrauterine infusion of 10 mg of β-NGF purified from llama semen diluted in 4 ml of PBS (spβ-NGF; n = 6)...
October 9, 2018: Theriogenology
A C S Castilho, F M Dalanezi, F F Franchi, C A Price, J C P Ferreira, E Trevisol, J Buratini
The aim of this study was to determine the expression of fibroblast growth factor 22 (FGF22) in the bovine corpus luteum (CL) and to investigate the effects of in vivo total or partial cloprostenol-induced luteolysis on the mRNA abundance of FGF22 and its receptor, FGFR1B. Corpora lutea at different stages of development were then dissected from abattoir ovaries (n = 10/stage); a portion of the tissue samples was fixed in paraformaldehyde and the remaining samples were homogenized and subjected to total RNA extraction...
October 5, 2018: Theriogenology
O Merino, R Sánchez, M B Gregorio, F Sampaio, J Risopatrón
Vitamin D (VD) is involved in many functions of the reproductive system male. The intake of diets high-fat and vitamin D deficiency (VD- ) can cause morphological and physiological changes in testis that relate to infertility in the male. However, its effects on sperm quality and in vivo fertility have been little studied. This study analyzed the effects of fat and VD on sperm quality and in vivo fertility in sperm of Sprague-Dawley rats. The rats were divided into four groups: G1: control diet with vitamin D (C/VD+ ), G2: control diet without vitamin D (C/VD- ), G3: high-fat diet with vitamin D (HF/VD+ ) and G4: high-fat diet without vitamin D (HF/VD- ) and were fed for 3 months...
September 27, 2018: Theriogenology
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