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Theriogenology

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https://www.readbyqxmd.com/read/27919445/effective-production-of-recipient-male-pigs-for-spermatogonial-stem-cell-transplantation-by-intratesticular-injection-with-busulfan
#1
Zhuoheng Lin, Jiajing Bao, Qunfang Kong, Yaofu Bai, Fenhua Luo, Zhou Songyang, Yingji Wu, Junjiu Huang
Germ cell transplantation has facilitated spermatogonial stem cell (SSC) and spermatogenesis research and shown great potential in the seed-breeding of domestic livestock. However, little progress has been made in large animals, primarily reflecting the difficulties in preparing sterile recipients. Here, we developed a novel protocol to prepare recipient pigs through the direct injection of busulfan into the cavum vaginale of the scrotums of Landrace-Large bi-crossbreeding male pigs and Seghers male pigs, two economically-important types of pigs, to eliminate endogenous spermatogonia...
November 1, 2016: Theriogenology
https://www.readbyqxmd.com/read/27793457/effect-of-follicular-aging-on-atp-content-and-mitochondria-distribution-in-bovine-oocytes
#2
Dinesh Dadarwal, Fernanda C F Dias, Gregg P Adams, Jaswant Singh
Hereford heifers were assigned randomly to three superstimulation groups and given FSH for 4 days in the short FSH group (n = 5) and FSH starvation group, (n = 5) or for 7 days in the long FSH group (n = 4). In vivo oocyte maturation was induced with LH given 12 hours after the last FSH treatment in short and long FSH groups and 84 hours after the last FSH treatment in the FSH starvation group. The ovaries were removed by colpotomy 18 to 20 hours after LH treatment to aspirate cumulus-oocyte complexes (COCs) from follicles 8 mm or greater...
October 3, 2016: Theriogenology
https://www.readbyqxmd.com/read/27793453/cholesterol-loaded-cyclodextrins-prevent-cryocapacitation-damages-in-buffalo-bubalus-bubalis-cryopreserved-sperm
#3
Valentina Longobardi, Giuseppe Albero, Carolina De Canditiis, Angela Salzano, Antonio Natale, Anna Balestrieri, Gianluca Neglia, Giuseppe Campanile, Bianca Gasparrini
The aim of this study was to investigate the effect of cholesterol-loaded cyclodextrins (CLC) on motility, viability, capacitation status, and in vivo fertility of buffalo frozen-thawed sperm. After the initial semen assessment, buffalo sperm were diluted in BULLXcell extender containing 0- (control), 1.5-, and 3-mg/mL CLC and cryopreserved. At thawing, sperm motility was evaluated by phase contrast microscopy, and viability-capacitation status was assessed by Hoechst 33258-chlortetracycline (CTC) assay. Capacitation status was also evaluated by an indirect immunofluorescence assay to localize phosphotyrosine-containing proteins...
October 3, 2016: Theriogenology
https://www.readbyqxmd.com/read/27746003/expression-and-localization-of-cysteine-rich-secretory-protein-3-crisp-3-in-the-prepubertal-and-postpubertal-male-horse
#4
C E Fedorka, K E Scoggin, E L Squires, B A Ball, M H T Troedsson
The seminal plasma protein, cysteine-rich secretory protein-3 (CRISP-3), has been correlated with increased fertility and first-cycle conception rates, and has been suggested to be involved in the modulation of polymorphonuclear neutrophil and phagocytosis of spermatozoa during the inflammatory response to breeding in the horse. Previous research demonstrated that equine CRISP-3 is located in both the ampulla of the vas deferens and the seminal vesicles. However, this was done with nonquantitative laboratory techniques...
September 21, 2016: Theriogenology
https://www.readbyqxmd.com/read/27842717/mhc-correlated-preferences-in-diestrous-female-horses-equus-caballus
#5
D Burger, C Meuwly, E Marti, H Sieme, M Oberthür, J Janda, S Meinecke-Tillmann, C Wedekind
Genes of the major histocompatibility complex (MHC) have been shown to influence communication in many vertebrates, possibly with context-specific MHC-correlated reactions. Here we test for MHC-linked female preferences in the polygynous horse (Equus caballus) by repeatedly exposing 19 mares to a group of seven sexually experienced stallions. Each mare was tested four times during two consecutive reproductive cycles, twice during estrus and twice during diestrus. Male plasma testosterone concentrations were determined from weekly blood samples, and equine leukocyte antigen (ELA) class I and II alleles were determined serologically at the end of the experiments...
September 20, 2016: Theriogenology
https://www.readbyqxmd.com/read/27743691/influences-of-exogenous-melatonin-on-the-oocyte-growth-and-oxidative-status-of-ovary-during-different-reproductive-phases-of-an-annual-cycle-in-carp-catla-catla
#6
Pradip Mondal, Kazi Nurul Hasan, Palash Kumar Pal, Saumen Kumar Maitra
The present study aimed to evaluate antioxidant role of melatonin in determining seasonality of ovarian growth in adult carp Catla catla. Accordingly, an identical regimen of exogenous melatonin administration (100 μg/100 g body weight per day for 15 days) was followed during the preparatory, prespawning, and spawning phases of an annual reproductive cycle. The study did not include postspawning phase, when the ovaries were completely regressed and devoid of any healthy growing follicles. The ovarian response was evaluated by determining relative number of developing oocytes as well as measuring the levels of melatonin, oxidative stress (using malondialdehyde [MDA] as the marker), both enzymatic (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], and glutathione S-transferase [GST]) and nonenzymatic (reduced glutathione [GSH]) antioxidants in the ovarian homogenates...
September 19, 2016: Theriogenology
https://www.readbyqxmd.com/read/27771114/ovarian-dysfunction-associated-with-zona-pellucida-based-immunocontraceptive-vaccines
#7
Carolynne J Joonè, Martin L Schulman, Henk J Bertschinger
Despite more than 40 years of research into zona pellucida (ZP)-based vaccines, relatively little is known about their mechanism of action. Early research demonstrated precipitation of ZP glycoproteins by antiovarian antiserum, rendering oocytes resistant to sperm binding in vitro. Subsequent work showed significantly decreased fertilization rates following passive immunization, sparking interest in anti-ZP immunocontraception for human and animal use. The primary mechanism of action of ZP vaccines is generally considered to be an antibody-mediated interference with sperm-oocyte binding and/or fertilization...
September 17, 2016: Theriogenology
https://www.readbyqxmd.com/read/27863702/detecting-asymptomatic-rams-infected-with-mycoplasma-agalactiae-in-ovine-artificial-insemination-centers
#8
Miranda Prats-van der Ham, Juan Tatay-Dualde, Christian de la Fe, Ana Paterna, Antonio Sánchez, Juan Carlos Corrales, Antonio Contreras, Ángel Gómez-Martín
Mycoplasma agalactiae (Ma) is the main causative agent of ovine contagious agalactia, which is a serious disease of small ruminants. In endemic areas, its most common clinical situation consists of chronically infected herds, and asymptomatic infected individuals represent an epidemiological risk regarding the transmission of this disease. The aim of this work was to detect the presence of asymptomatic rams infected with Ma in different artificial insemination centers, and to determine the most effective way to identify these individuals so as to implement adequate surveillance protocols...
September 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27751602/heat-stress-induced-autophagy-promotes-lactate-secretion-in-cultured-immature-boar-sertoli-cells-by-inhibiting-apoptosis-and-driving-slc2a3-ldha-and-slc16a1-expression
#9
Zi-Qiang Bao, Ting-Ting Liao, Wei-Rong Yang, Yi Wang, Hong-Yan Luo, Xian-Zhong Wang
This study aimed to determine whether heat stress (HS) could induce autophagy in immature boar Sertoli cells (SCs) and test whether HS-induced autophagy could regulate lactate secretion by SCs. Cultured immature boar SCs were incubated at 43 °C for 30 minutes. The ratio of LC3B-II to LC3B-I and the mRNA transcript levels of LC3B showed time-dependent changes 0 to 48 hours after HS treatment, which peaked at 24 hours and increased by 30.25% or 260%, respectively, compared with control SCs. The density of autolysosomes, which were labeled with a red dye, was higher at 24 hours than at any other time point...
September 15, 2016: Theriogenology
https://www.readbyqxmd.com/read/27771115/comparing-strategies-to-synchronize-estrus-before-fixed-time-artificial-insemination-in-primiparous-2-year-old-beef-cows
#10
J M Abel, B E Bishop, J M Thomas, M R Ellersieck, S E Poock, M F Smith, D J Patterson
Two experiments evaluated controlled internal drug release (CIDR)-based protocols to synchronize estrus in primiparous 2-year-old beef cows. In each experiment, treatments were balanced according to body condition score and days postpartum. Experiment 1 compared the 14-day CIDR-PG (14-d) and 7-day CO-Synch + CIDR (7-d) protocols on the basis of estrous response, pregnancy rates after fixed-time artificial insemination (FTAI), and final pregnancy rate. Cows assigned to 14-d (n = 355) received a CIDR insert on Day 0 with removal on Day 14...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27769573/relationships-between-mitochondrial-dna-content-mitochondrial-activity-and-boar-sperm-motility
#11
Huiduo Guo, Yabin Gong, Bin He, Ruqian Zhao
Energy produced by mitochondria via oxidative phosphorylation (OXPHOS) is essential for mammalian sperm motility. Mammalian mitochondrial DNA (mtDNA)-encoded proteins are subunits of the OXPHOS system. Paradoxically, there are less mitochondrial and mtDNA contents in motile sperm than less motile sperm. Here, mature boar sperm was used as a model to investigate the relationships between mtDNA content, mitochondrial activity, and sperm motility. Motile and less motile sperm were separated by centrifugation on a discontinuous percoll density gradient...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27743689/ovulation-synchronization-with-estradiol-benzoate-or-gnrh-in-a-timed-artificial-insemination-protocol-in-buffalo-cows-and-heifers-during-the-nonbreeding-season
#12
N A T Carvalho, J G Soares, D C Souza, J R G Maio, J N S Sales, B Martins Júnior, R C Macari, M J D'Occhio, P S Baruselli
The aim of this study was to compare estradiol benzoate (EB) and GnRH for the induction of ovulation in a TAI protocol in buffalo during the nonbreeding season. In experiment 1, 141 buffaloes (56 cows and 85 heifers) received an intravaginal P4 device (1.0 g) plus EB (2.0-mg, intramuscular [im]) at random stage of the estrous cycle (Day 0). On Day 9, the P4 device was removed, and buffaloes were given PGF2α (0.53-mg im sodium cloprostenol) plus eCG (400-IU im). Buffaloes were then randomly allocated to one of three groups and treated as follows: EB24 (n = 47), EB (1...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27742403/effect-of-histone-acetylation-modification-with-mgcd0103-a-histone-deacetylase-inhibitor-on-nuclear-reprogramming-and-the-developmental-competence-of-porcine-somatic-cell-nuclear-transfer-embryos
#13
Long Jin, Hai-Ying Zhu, Qing Guo, Xiao-Chen Li, Yu-Chen Zhang, Cheng-Du Cui, Wen-Xue Li, Zheng-Yun Cui, Xi-Jun Yin, Jin-Dan Kang
Cloning remains as an important technique to enhance the reconstitution and distribution of animal population with high-genetic merit. One of the major detrimental factors of this technique is the abnormal epigenetic modifications. MGCD0103 is known as a histone deacetylase inhibitor. In this study, we investigated the effect of MGCD0103 on the in vitro blastocyst formation rate in porcine somatic cell nuclear transferred (SCNT) embryos and expression in acetylation of the histone H3 lysine 9 and histone H4 lysine 12...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27729112/caprine-ovarian-follicle-requirements-differ-between-preantral-and-early-antral-stages-after-ivc-in-medium-supplemented-with-gh-and-vegf-alone-or-in-combination
#14
J Cadenas, J Leiva-Revilla, L A Vieira, L B Apolloni, F L N Aguiar, B G Alves, C H Lobo, A P R Rodrigues, G A Apgar, J Smitz, J R Figueiredo, C Maside
The aim of the present study was to evaluate the effect of growth hormone (GH) and vascular endothelial growth factor (VEGF) added alone, sequentially or in combination, in the presence of insulin at physiological concentration (10 ng/mL) on the IVC of two different follicular categories: preantral (experiment 1; Exp.1) and early antral (experiment 2; Exp.2). Isolated follicles were individually cultured for 24 (Exp.1) and 18 days (Exp.2) in the following treatments: αMEM(+) (Control), or Control medium supplemented with 50 ng/mL GH (GH), 100 ng/mL VEGF (VEGF), the combination of both (GH + VEGF), GH during the first 12 days and VEGF from Day 12 until the end of the culture (GH/VEGF) and vice versa (VEGF/GH)...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27729111/vascularization-and-vegf-expression-altered-in-bovine-yolk-sacs-from-ivf-and-nt-technologies
#15
Andrea Maria Mess, Ana Claudia Oliveira Carreira, Cláudia Marinovic de Oliveira, Paula Fratini, Phelipe Oliveira Favaron, Rodrigo da Silva Nunes Barreto, Christiane Pfarrer, Flávio Vieira Meirelles, Maria Angelica Miglino
Reproductive technologies are widely used in cattle, although many are associated with high-embryonic mortality, especially during early gestation, when the yolk sac undergoes macroscopic changes in structure. We hypothesized that vasculogenesis and angiogenesis are affected, thereby affecting embryonic and placental differentiation. To test this, we studied yolk sac development and gene expression of the vascular endothelial growth factor system (VEGF-A, VEGFR-1/Flt-1, VEGFR-2/KDR). Samples from Days 25 to 40/41 of pregnancy from control cattle (n = 8) and from pregnancies established with IVF, (n = 7) or somatic cell nuclear transfer/clones (n = 5) were examined by histology, immunohistochemistry, and quantitative reverse transcriptase PCR...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27707547/continuous-contact-with-females-in-estrus-throughout-the-year-enhances-testicular-activity-and-improves-seminal-traits-of-male-goats
#16
Julia Giriboni, Lorena Lacuesta, Rodolfo Ungerfeld
The "female effect" consists in the stimulation of males' reproductive activity by different signals emitted by females. This stimulation leads to endocrine and behavioral changes that may modify the seasonal pattern of male ruminants. The aims of this experiment were (1) to describe the local reproductive seasonal pattern of Gabon bucks and (2) to determine if continuous chemical, auditory, and visual contact with does in estrus enhances bucks' testicular activity and improves seminal traits throughout the year and modify their seasonal pattern...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27707545/surgical-embryo-collection-but-not-nonsurgical-embryo-transfer-compromises-postintervention-prolificacy-in-sows
#17
C A Martinez, A Nohalez, I Parrilla, J L Vazquez, J Roca, C Cuello, H Rodriguez-Martinez, E A Martinez, M A Gil
Recent advances in nonsurgical deep uterine (NsDU) embryo transfer (ET) technology allow the noninvasive transfer of porcine embryos into recipients, overcoming the most important impediment for commercial ET in this species. Although many factors in the porcine ET-field have been recently evaluated, many others remain to be explored. We investigated here the future reproductive performance of donors and recipients after artificial insemination subsequent to the default surgical embryo recovery approach and to the NsDU-ET procedure, respectively...
September 13, 2016: Theriogenology
https://www.readbyqxmd.com/read/27865419/gpr39-is-region-specifically-expressed-in-mouse-oviduct-correlating-with-the-zn-2-distribution
#18
Jingqiao Qiao, Huashan Zhao, Ying Zhang, Hongying Peng, Qi Chen, He Zhang, Xueying Zheng, Yaping Jin, Hemin Ni, Enkui Duan, Yong Guo
G-protein-coupled receptor 39 (GPR39) plays a role in cellular and physiological processes, including insulin secretion, cell death inhibition, wound healing, and obesity. Increasing evidence suggests that GPR39 is potently stimulated by zinc ions (Zn(2+)) and is therefore considered a putative Zn(2+) receptor. Given the importance of Zn(2+) in the reproductive system, we proposed that GPR39 might have a functional role in the reproductive system. However, the localization of GPR39 in the reproductive system remains unknown...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27865418/characterizing-emergence-and-divergence-in-the-first-follicular-wave-in-a-tropically-adapted-bos-taurus-breed
#19
Rodrigo Dorneles Tortorella, Guilherme de Paula Nogueira, Mairon Rocha Modesto, Priscilla Cristine Passoni Silva, Joseane Padilha da Silva, Jairo Pereira Neves, Alexandre Floriani Ramos
Tropically adapted Bos taurus breeds are descended from the cattle which were brought from the Iberian Peninsula to Latin America by the colonizers and which adapted over time to local conditions. These breeds have become a genetic treasure that must be preserved. The objective of this study was to characterize ovarian follicular emergence and divergence in tropically adapted B. taurus cows and heifers. Cyclic heifers (n = 11) and nonlactating, multiparous cows (n = 11) were examined at 8-hour intervals using ultrasonography starting on the day following the final application of PGF2α, which was used to synchronize the estrous cycle, and ending 5 days after ovulation...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27865417/knock-down-of-apoptosis-inducing-factor-gene-protects-endoplasmic-reticulum-stress-mediated-goat-granulosa-cell-apoptosis
#20
Diqi Yang, Tingting Jiang, Pengfei Lin, Huatao Chen, Lei Wang, Nan Wang, Fan Zhao, Aihua Wang, Yaping Jin
The apoptosis of granulosa cells is the main cause of follicular atresia, and endoplasmic reticulum (ER) stress is involved in the apoptosis of granulosa cells. Apoptosis inducing factor (AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation, but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown. The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells mediated by ER stress...
January 15, 2017: Theriogenology
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