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Theriogenology

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https://www.readbyqxmd.com/read/28601162/immature-and-mature-sperm-morphometry-in-fresh-and-frozen-thawed-falcon-ejaculates
#1
S Villaverde-Morcillo, A J Soler, M C Esteso, C Castaño, A Miñano-Berna, F Gonzalez, J Santiago-Moreno
Sperm morphometry is one characteristic which may be useful in prediction of fertility and sperm freezability in a species. Knowledge of the sperm characteristics of the ejaculate and the morphometric descriptors is necessary to effectively develop sperm cryopreservation. The aim of the current study was to provide a general description of the sperm from two falcon species (Peregrine falcon Falco peregrinus peregrinus/brookei and Gyrfalcon Falco rusticolus) including immature sperm, sperm head morphometric descriptors, and the existence of mature sperm subpopulations...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601161/effective-use-of-sexedultra%C3%A2-sex-sorted-semen-for-timed-artificial-insemination-of-beef-heifers
#2
J M Thomas, J W C Locke, R Vishwanath, J B Hall, M R Ellersieck, M F Smith, D J Patterson
An experiment was designed to evaluate the relative fertility of SexedULTRA(TM) sex-sorted semen compared to conventional, non-sex-sorted semen when used among beef heifers in conjunction with split-time AI following the 14-d CIDR-PG protocol. Units of conventional semen were generated with 25.0 × 10(6) live cells per 0.5 ml straw prior to freezing, and units of sex-sorted semen were generated using the SexedULTRA(TM) Genesis III sorting technology with 4.0 × 10(6) live cells per 0.25 ml straw prior to freezing...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601160/sperm-collection-by-transrectal-ultrasound-guided-massage-of-the-accessory-sex-glands-is-less-stressful-than-electroejaculation-without-altering-sperm-characteristics-in-conscious-goat-bucks
#3
Silvia Abril-Sánchez, Aline Freitas-de-Melo, Florencia Beracochea, Juan Pablo Damián, Julia Giriboni, Julián Santiago-Moreno, Rodolfo Ungerfeld
In anesthetized non-domestic ruminants transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) is an alternative method to collect semen slightly less stressful than electroejaculation (EE). However, some sperm characteristics are better when semen is collected with EE than with TUMASG. As anesthesia reduces the response to stressors, the advantages of TUMASG may be reduced in anesthetized animals, and thus, TUMASG may be even more advantageous in conscious animals. Therefore, the aim of the present study was to compare the stress response and the characteristics of the sperm collected with TUMASG and EE in conscious goat bucks...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601159/abnormal-pregnancies-associated-with-deviation-in-progestin-and-estrogen-profiles-in-late-pregnant-mares-a-diagnostic-aid
#4
Mitsumori Shikichi, Katsuroku Iwata, Katsumi Ito, Daisuke Miyakoshi, Harutaka Murase, Fumio Sato, Kenji Korosue, Shunichi Nagata, Yasuo Nambo
Measurement of progestines and estrogens in maternal plasma has been advocated as an indicator of foeto-placental function. The goals of the present study were as follows: to determine progestin and estrogen concentrations in mares with normal and abnormal pregnancies during late gestation, evaluate the diagnostic value of hormone concentrations, and investigate the relationship between hormone concentrations after treatment and the survival of foals. Blood samples were collected monthly from 459 pregnant mares between Day 201 of gestation and foaling...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601158/effect-of-corifollitropin-alfa-supplemented-with-or-without-lh-on-ovarian-stimulation-and-embryo-viability-in-rabbit
#5
María Pilar Viudes-de-Castro, Francisco Marco-Jiménez, José Isaí Cedano-Castro, José Salvador Vicente
There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601157/protein-based-flushing-related-blood-urea-nitrogen-effects-on-ovarian-response-embryo-recovery-and-embryo-quality-in-superovulated-ewes
#6
İrfan Tur, Dursun Ali Dínç, Ahmet Semacan
The present study is the first report that evaluates effects of nutritional effects of flushing with differing diet crude protein ratios on blood urea nitrogen (BUN) levels, related some reproductive parameters and embryo quality in ewe. During mating season, before synchronization protocol ewes were fed on alfalfa hay and additive concentrate feeding as flushing. Intra vaginal FGA containing sponges applied for 12 days for the purpose of synchronization and pFSH was administered by 8 declining doses for the purpose of superovulation...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601156/ovopel-and-carp-pituitary-extract-for-the-reproductive-induction-of-colossoma-macropomum-males
#7
Edenilce de Fátima Ferreira Martins, Danilo Pedro Streit, Janessa Sampaio de Abreu, Ruy Alberto Caetano Corrêa-Filho, Carlos Antonio Lopes de Oliveira, Nelson Mauricio Lopera-Barrero, Jayme Aparecido Povh
The objective of this study was to evaluate Ovopel and carp pituitary extract (CPE) in the reproductive induction of Colossoma macropomum males. Nine treatments were tested in triplicate, totaling 27 experimental units. C. macropomum breeders were subjected to the following treatments: 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, and 0.7 Ovopel pellet/kg; 2.5 mg CPE/kg (traditional protocol); and a control treatment (no hormone). Breeders under hormone treatment produced a larger (P < 0.05) semen volume (2.4 ± 0...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601155/boar-genotype-as-a-factor-shaping-age-related-changes-in-semen-parameters-and-reproduction-longevity-simulations
#8
Damian Knecht, Anna Jankowska-Mąkosa, Kamil Duziński
The aim of this study was a detailed analysis of the boar genotypes used in AI stations with an indication of their production capacity, including age and a precise analysis of their culling time and reason. The study included 334 boars: 81 Polish Large White (PLW), 108 Polish Landrace (PL), 49 Pietrain (P), 56 Duroc × Pietrain (D × P) and 40 Hampshire × Pietrain (H × P). Semen volume, spermatozoa concentration, total number of spermatozoa, number of motile spermatozoa, and number of insemination doses were analyzed...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601154/spermatozoal-transcripts-expression-levels-are-predictive-of-semen-quality-and-conception-rate-in-bulls-bos-taurus
#9
Sivashanmugam Parthipan, Sellappan Selvaraju, Lakshminarayana Somashekar, Arunachalam Arangasamy, Muniandy Sivaram, Janivara Parameswaraiah Ravindra
Spermatozoal transcripts expression levels could be used to assess fertility potential of a male. The objective of the present study was to elucidate the predictive ability of the expression levels of growth, apoptosis and homeostasis regulating transcripts on sperm functions and fertility. The expression levels of spermatozoal RNA isolated from the neat semen samples were related to the good (discarded ejaculate, <25%; n = 7) and poor (discarded ejaculate, >40%, n = 6) quality semen producer and bulls (n = 12) with known conception rate...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601153/equine-chorionic-gonadotropin-increases-fertility-of-grazing-dairy-cows-that-receive-fixed-time-artificial-insemination-in-the-early-but-not-later-postpartum-period
#10
Alexandre B Prata, Guilherme C S Pontes, Pedro Leopoldo J Monteiro, Jéssica N Drum, Milo C Wiltbank, Roberto Sartori
This study evaluated effects of equine chorionic gonadotropin (eCG) on fertility of 679 crossbred (Bos taurus x Bos indicus) lactating grazing cows synchronized for fixed-time AI (FTAI). At a random day of the estrous cycle cows received an intravaginal progesterone (P4) implant, 2 mg estradiol benzoate (EB) and 100 μg gonadorelin (D0-AM). On D7-AM, cows received 0.5 mg sodium cloprostenol and were randomly assigned into two treatments: eCG (n = 340; 400 IU eCG on D7), or Control (n = 339; no eCG). On D8-PM, P4 implants were removed and cows received 0...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601152/identification-and-expression-of-x-linked-inhibitor-of-apoptosis-protein-during-follicular-development-in-goat-ovary
#11
Peng Han, Haiyun Xin, Jiayin Peng, Jinxing Hou, Lei Zhang, Yuxuan Song, Guang Li, Binyun Cao, Xiaopeng An
X-linked inhibitor of apoptosis protein (XIAP), an endogenous of inhibitor of caspases, plays crucial roles in regulating ovarian granulosa cell apoptosis during follicular atresia. The aim of the present study was to determine the presence and localization of XIAP in the goat ovary and its expression level during follicular development. The full length cDNA of XIAP from goat ovary cells was cloned using reverse transcription PCR. A total of 497 amino acid residues were encoded by open reading frame and had high identity with homologous sequences from other mammals...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601151/effect-of-season-and-age-on-scrotal-circumference-testicular-parameters-and-endocrinological-profiles-in-mithun-bulls
#12
P Perumal, N Savino, C T R Sangma, S Chang, T Z T Sangtam, M H Khan, G Singh, Brijesh Kumar, D Yadav, N Srivastava
Mithun is a domesticated free-range bovine species primarily used as a meat animal and is a pride of North Eastern Hilly regions of India. The present study was conducted to measure the effect of seasons on scrotal & testicular biometrics and endocrinological profiles for different age groups at different seasons in mithun bulls. A total of 30 mithun males were selected from the mithun breeding farm, ICAR-NRC on Mithun, Medziphema, Nagaland, India and were equally distributed into five groups based on their age...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601150/progress-and-challenges-of-fish-sperm-vitrification-a-mini-review
#13
REVIEW
Miaomiao Xin, Mohammad Abdul Momin Siddique, Borys Dzyuba, Rafael Cuevas-Uribe, Anna Shaliutina-Kolešová, Otomar Linhart
To survive low temperature is required for a long-term storage (cryopreservation), cells should be vitrified to a state in which intracellular water is solidified without ice crystal formation. Two different approaches are described for fish sperm cryopreservation: 1) sperm conventional cryopreservation, in which extracellular water is partially crystallized and 2) sperm vitrification, in which both intra- and extra-cellular liquids are vitrified. Sperm vitrification has been applied to some fish species with limited success...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601149/cells-expressing-cd4-cd8-mhcii-and-endoglin-in-the-canine-corpus-luteum-of-pregnancy-and-prepartum-activation-of-the-luteal-tnf%C3%AE-system
#14
Renata M Nowaczyk, Ewelina Jursza-Piotrowska, Aykut Gram, Marta J Siemieniuch, Alois Boos, Mariusz P Kowalewski
In the dog, knowledge about involvement of the immune system in controlling luteal function is restricted to observations showing a time-dependent invasion of immune cells into the corpus luteum (CL) of non-pregnant bitches. Therefore, this study investigated the presence of CD4-, CD8-, MHCII- and endoglin-expressing cells in CL collected throughout pregnancy from pre-implantation until prepartum luteolysis. Immunohistochemistry and semi-quantitative RT-PCR were applied. The time-dependent expression of CD4, CD8 and endoglin was more strongly related to formation of the CL, whereas MHCII was induced during luteolysis...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601148/leptin-supplementation-in%C3%A2-vitro-improved-developmental-competence-of-buffalo-oocytes-and-embryos
#15
Bibhudatta S K Panda, Sriti Pandey, Anjali Somal, Mehtab S Parmar, Irfan A Bhat, Indu Baiju, Mukesh K Bharti, G Sai Kumar, Vikash Chandra, G Taru Sharma
The aim of the present study was to determine potential role of leptin on in vitro developmental competence of buffalo oocytes and embryos. Slaughterhouse derived culture grade buffalo cumulus oocyte complexes (COCs) were matured in vitro (IVM) with leptin (10 ng/ml) or without leptin (control). In each experiment, a pool of matured COCs was used for further in vitro embryo production and another pool of COCs was used for cumulus cells and mature oocytes isolation to study the relative mRNA expression of developmentally important genes...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601147/deep-anestrous-mares-under-natural-photoperiod-treated-with-recombinant-equine-fsh-refsh-and-lh-relh-have-fertile-ovulations-and-become-pregnant
#16
Geraldine A Meyers-Brown, Meaghan C Loud, John C Hyland, Janet F Roser
The most common equine breeding practice to decrease the time to the first ovulation of the year is to use artificial lights starting December 1 in the Northern Hemisphere. It can take 60-90 d for this lighting regimen to induce a fertile ovulation. The success rate for pharmaceutical compounds to carry out the same process has been variable. One compound that did induce an early ovulation was recombinant equine follicle stimulating hormone (reFSH), but neither pregnancy nor cyclicity was established in that study...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601146/genetic-component-of-sensitivity-to-heat-stress-for-nonreturn-rate-of-brazilian-holstein-cattle
#17
M L Santana, A B Bignardi, G Stefani, L El Faro
The objectives of the present study were: 1) to investigate variation in the genetic component of heat stress for nonreturn rate at 56 days after first artificial insemination (NR56); 2) to identify and characterize the genotype by environment interaction (G × E) due to heat stress for NR56 of Brazilian Holstein cattle. A linear random regression model (reaction norm model) was applied to 51,748 NR56 records of 28,595 heifers and multiparous cows. The decline in NR56 due to heat stress was more pronounced in milking cows compared to heifers...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28601145/ovarian-development-and-the-reproductive-profile-of-astyanax-altiparanae-teleostei-characidae-over-one-year-applications-in-fish-farming
#18
Mônica Cassel, Chayrra Chehade, Giovana Souza Branco, Danilo Caneppele, Elizabeth Romagosa, Maria Inês Borella
Astyanax altiparanae is a Brazilian species of substantial commercial, environmental and scientific importance; however, existing studies on its reproduction do not seem to provide enough details. In light of the increasing use of this species in fish farming and the need for basic studies for the development of new production technologies, we describe the structural and ultrastructural characteristics of the ovaries of A. altiparanae, and characterize the species' reproductive cycle. Females were collected monthly from March 2013 to February 2014, and reproductive management began in October 2013...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28583616/effects-of-season-on-placental-foetal-and-neonatal-development-in-horses
#19
Elisabeth Beythien, Christine Aurich, Manuela Wulf, Jörg Aurich
Seasonal changes in metabolic rate have been shown in horses and we hypothesized that this leads to the birth of smaller foals early in the year. Mares and their foals were assigned to three groups by day of foaling within the year (e.g. 1 January = day 1): Group 1 (n = 10) day 40-65, group 2 (n = 8) day 67-92, group 3 (n = 9) day 94-121. Groups did not differ with regard to parity. In foals, height at withers and body weight were determined on days 1-5 and weekly until 12 weeks of age. Chest circumference, distances fetlock to carpus, carpus to elbow, poll to nose and crown-rump length were determined on day 5 and weekly until 12 weeks of age...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583615/identification-of-differentially-expressed-genes-in-early-developmental-ovary-of-yellow-river-carp-cyprinus-carpio-var-using-suppression-subtractive-hybridization
#20
Wan-Wan Zhang, Yong-Fang Jia, Fang Wang, Qi-Yan Du, Zhong-Jie Chang
Ovary development appears to be under polygenic control, and is influenced by multiple genetic factors that may vary from organism to organism. To gain a better insight into the molecular mechanisms of carp ovary development, Suppression Subtractive Hybridization (SSH) DNA libraries in two species of Yellow River carp were analyzed. Primordial gonads and stage II ovaries were used as testers, and adult ovaries as drivers. One hundred and fifty differentially-expressed candidate genes were examined by Southern blot microarray hybridization...
July 15, 2017: Theriogenology
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