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Elina V García, María E Oliva, Jean G LeBlanc, Antonio D Barrera
Recent studies have demonstrated that the oviductal environment plays an active role in modulating the epigenetic marks of the preimplantation embryo genome, but the molecular factors that mediate this epigenetic effect are unknown. Folate is a well-known epi-nutrient that can impact on cell epigenetic machinery during embryonic and fetal development. However, the study of this epi-nutrient in the oviduct is still limited. The present study was conducted to confirm the presence and physiological concentration of folate in bovine oviductal fluid (OF) and to determine if bovine oviduct epithelial cells (BOECs) are able to regulate the uptake of this micronutrient...
July 10, 2018: Theriogenology
Rafael Dos Santos de Andrade, Frederico Ozanan Barros Monteiro, Hani Rocha El Bizri, Luciana Pantoja, Richard Bodmer, João Valsecchi, Pedro Mayor
The white-lipped peccary (Tayassu pecari) is an endangered large-sized Neotropical ungulate that is one of the most hunted mammals in the Amazon. Here, we used two embryos and 102 white-lipped peccary fetuses originated from animals hunted for subsistence in the Peruvian and Brazilian Amazon to describe the intrauterine development of external and internal morphology of this Neotropical ungulate. Logistic regressions were used to estimate the probability of occurrence of main external characteristics in relation to the total dorsal length (TDL), while multiple linear and non-linear regressions were conducted to assess the relationship between external and visceral biometry with TDL...
July 10, 2018: Theriogenology
J M Guillen-Muñoz, C A Meza-Herrera, R Rivas-Muñoz, Z Zuñiga-Garcia, G Calderon-Leyva, Miguel Mellado, Francisco G Veliz-Deras
This research evaluated the possible influence of exposure of male goats to estrogenized female goats ("female effect") upon males' sexual behavior [appetitive (ASB) & consummatory (CSB)], as well as the induction of reproductive activity of crossbred dairy female goats exposed to such treated males ("male effect") during the early and deep anestrous periods. Crossbred dairy adult male goats (n = 12; 24-48 mo. old) and 80 anovulatory crossbred dairy adult female goats (34-50 mo. old) were used during two experimental periods: March to April and April to May...
July 7, 2018: Theriogenology
Ismael Lamas-Toranzo, Eva Pericuesta, Pablo Bermejo-Álvarez
In vitro maturation (IVM) leads to reduced developmental rates compared to the use of in vivo matured oocytes. This reduction can be attributed to the suboptimal environment experienced during IVM, but the use of incompetent oocytes also plays a significant role. The objective of this study has been to characterize the mitochondrial and metabolic differences between competent and incompetent bovine oocytes selected prior to IVM based on Brilliant Cresyl Blue (BCB) staining. BCB selection allowed to sort two populations of cumulus-oocyte complexes (COCs) exhibiting diverse developmental competence despite showing a similar size and thereby being morphologically undistinguishable otherwise...
July 7, 2018: Theriogenology
Raul A Gonzalez-Castro, Elaine M Carnevale
Limited clinical information is available regarding sperm population parameters that are important for use with equine intracytoplasmic sperm injection (ICSI). Therefore, the appropriateness of a sample of sperm is typically not known before ICSI. The aim of our study was to determine which sperm population characteristics were predictive of ICSI outcome. Frozen-thawed sperm samples (n = 114) from 37 stallions in a clinical program were analyzed after ICSI for percentages of normal morphology (MORPH+), live as assessed by eosin/nigrosin stain (LIVE+), membrane intact as assessed by hypoosmotic swelling test (HOS+), and DNA fragmentation determined by sperm chromatin dispersion (DNA-)...
July 5, 2018: Theriogenology
Dong-Hyuk Jeong, Jeong-Ho Kim, Ki-Jeong Na
The Amur leopard cat (Prionailurus bengalensis euptilurus) is a globally endangered species, and there is thus an urgent need to increase its population. The objectives of this study were to: (1) confirm the utility of urethral catheterization (UC) for semen collection from Amur leopard cats; (2) investigate proper dilution media for fresh semen; and (3) investigate the effectiveness of sperm cryopreservation, including examining the effect of glycerol concentration. Six adult males in captivity (mean weight 5...
July 5, 2018: Theriogenology
Alejandro Maruri, Paula Romina Cruzans, María Soledad Lorenzo, María Fernanda Tello, Gabriela Maia Teplitz, María Clara Carou, Daniel Marcelo Lombardo
The coculture with somatic cells is an alternative to improve suboptimal in vitro culture (IVC) conditions and promote embryo development. Several cell types have been used for this purpose, but there is no information about using luteal cells in short-term coculture with embryos. Consequently, this study aimed to assess the effect of a short-term coculture of early bovine embryos-luteal cells on the in vitro development and embryo quality. Presumptive embryos were cultured from day 0 to day 2 in medium alone (control) or cocultured with bovine luteal cells (BLC-1)...
July 4, 2018: Theriogenology
Y X Fan, Z Wang, C F Ren, T W Ma, K P Deng, X Feng, F Z Li, F Wang, Y L Zhang
Nutritional intake and reproductive allocation are strongly associated and dietary energy restriction (ER) or surpluses can affect reproductive capacity. The objective of this study was to investigate the effect of energy levels on sheep testicular development. Three-month old Hu sheep were assigned to four groups, and fed diets containing different levels of energy (Control, maintenance energy; ER1, 85% maintenance energy; ER2, 70% maintenance energy; ER3, 55% maintenance energy). Two months later, half the sheep in each group were euthanized, whereas the remaining sheep were euthanized after a further 3 months feeding on a compensatory energy diet...
July 3, 2018: Theriogenology
Ariadna Corral, Marcin Balcerzyk, Miguel Gallardo, Christiani A Amorim, Ángel Parrado-Gallego, Ramón Risco
Cryopreservation and subsequent transplantation of ovarian tissue is the only option to preserve fertility in certain patients facing gonadotoxic treatment. So far, cryopreservation of ovarian tissue has been carried out mostly by a controlled rate slow cooling process, typically known as slow freezing. Even though there are still some concerns about the iatrogenic damage on the follicle population, this technique has been used in the more than 100 live births reported to date. It is well known that the control of the cryoprotectant loading in the tissue is crucial to in a cryopreservation procedure...
July 3, 2018: Theriogenology
Xue-Yu Xu, De Wu, Sheng-Yu Xu, Lian-Qiang Che, Zheng-Feng Fang, Bin Feng, Jian Li, Cai-Mei Wu, Yan Lin
To elucidate the role of microRNA (miRNA) during early testicular development, we constructed three small RNA libraries from boar testes at three timepoints. Utilizing Solexa deep sequencing technology, over 12 million reads were measured. Of a total of 263 known miRNAs, 159 were co-expressed in all libraries. Read counts of the top 20 most abundant miRNAs accounted for more than 75% of total known miRNAs, and in all libraries, miR-10b was the most abundant. Analysis of higher fold changes in miRNAs (|log2_fold changes| > 4) revealed seven miRNAs that were involved in testes development (miR-381, miR-205, miR-217, miR-146a-5p, miR-187, miR-215, and miR-195)...
July 3, 2018: Theriogenology
Jerica J J Rich, Emmalee J Northrop, Erin L Larimore, George A Perry
Previous research has indicated that multiple small doses of GnRH following CIDR removal increased circulating concentrations of estradiol. Therefore, our objective was to determine if a single small dose of GnRH (5 μg or 10 μg) at CIDR removal would impact expression of estrus and/or interval to estrus. Beef cows and heifers (n = 1620; n = 1057 cows, n = 563 heifers) were synchronized using the 7-day CO-Synch + CIDR protocol, and randomly assigned to one of three treatments (0, 5, or 10 μg of a GnRH analog at CIDR removal)...
June 30, 2018: Theriogenology
B A Rakha, M S Ansari, S Akhter, J Santiago-Moreno, E Blesbois
Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol...
June 28, 2018: Theriogenology
K A Meller, J Całka, M Kaczmarek, B Jana
Under physiological conditions, noradrenaline (NA) and adrenergic receptors (ARs) are implicated in the function of the uterus. The role of NA and the expression of ARs in the inflamed uterus is not fully understood. The aim of the present study was to determine the effect of inflammation on the levels of α1 (A, B, D)-, α2 (A, B, C)- and β (1, 2, 3)-ARs mRNA and protein expression and the localization of these receptors in the porcine uterus. On Day 3 of the estrous cycle (Day 0 of the study), 50 ml of either saline (group SAL) or E...
June 28, 2018: Theriogenology
Xiaofeng Sun, Fangming Xiu, Bo Pan, Yapeng Li, James T Haskins, Wei Shen, Julang Li
Antimicrobial peptides (AMP) are host defense peptides present in all species examined. The objective of the current study was to characterize the expression of a group of antimicrobial peptides in ovarian cells, and to investigate their expression response to pathogen ligands. It was found that while PG1 transcript was not detected in the ovary, the expression of BD2 is the highest in small follicle derived granulosa cells (SGC), and its expression decreases during follicular development to large follicle stage (LGC; p < 0...
June 28, 2018: Theriogenology
Marcello Raspa, Martin Fray, Renata Paoletti, Lluis Montoliu, Alessandro Giuliani, Ferdinando Scavizzi
The mouse is widely used for biomedical research and an increasing number of genetically altered models are currently generated, therefore centralized repositories are essentials to secure the important mouse strains that have been developed. We have previously reported that spermatozoa of wild type and mutant strains frozen using standard laboratory protocols can be transported in dry ice (-79 °C) for 7 days and safely stored in a -80 °C freezer for up to two years. The objective of this new study was to compare the effects of the freezing techniques using LN2 or -80 °C freezer on fertility of frozen-thawed mouse spermatozoa...
June 28, 2018: Theriogenology
Liang-Ting Tang, Xue-Qin Ran, Ning Mao, Fu-Ping Zhang, Xi Niu, Yi-Qi Ruan, Fan-Li Yi, Sheng Li, Jia-Fu Wang
Alternative splicing (AS) is an important mechanism for controlling gene expression, which regulates multiple biological processes in higher organisms. Chinese indigenous Xiang pig has distinctive biological characteristics, such as small size, early sexual maturity, lower litter size and not very clear exhibition of estrous behaviors. To further understand how AS responds to estrous cycles in Xiang pig, the genome-wide analysis of AS events was performed by RNA-seq method in Xiang pig ovaries at diestrous and estrous...
June 27, 2018: Theriogenology
M K Muhammad Aslam, Vinay K Sharma, Shashank Pandey, A Kumaresan, A Srinivasan, T K Datta, T K Mohanty, Savita Yadav
Associations between expression of some proteins in spermatozoa and fertility have been sought in recent years to identify the male fertility markers. Since the incidence of sub-fertility is high in crossbred bulls, the present investigation was carried out on high- and low-fertile crossbred bulls to identify fertility markers in spermatozoa through proteomics approach. Sperm proteome of high-fertile bulls were compared with low-fertile bulls using 2D-DIGE and MALDI-TOF-MS techniques and the results were validated with immuno-blotting...
June 27, 2018: Theriogenology
V L Maciel, M C Caldas-Bussiere, V Silveira, R S Reis, A F L Rios, C S Paes de Carvalho
The aim of this work was to evaluate the proteomic changes that occurred in the frozen-thawed bovine spermatozoa after the addition of l-arginine (L-arg) during in vitro sperm capacitation. Aspects related to the sperm capacitation pattern like membrane integrity, mitochondrial activity, sperm motility and vigor, and the sperm proteome were determined. These were respectively assessed by chlortetracycline staining, H342/PI, JC-1, light microscopy, and the proteomic abundance by nUPLC-MS/MS analysis. Frozen-thawed sperm from three Nellore bulls were capacitated in vitro for 3 h in sp-TALP medium supplemented with 20 μg/mL heparin (Control) or with 20 μg/mL heparin plus 1 mM L-arg (L-arg group)...
June 26, 2018: Theriogenology
S Akhter, B A Rakha, M S Ansari, S Iqbal, M Khalid
Egg yolk is used as a cryoprotectant in semen preservation. However, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. Therefore, study was conducted to identify the optimum level of pigeon egg yolk (PEY) in Tris citric acid (TCA) extender for freezability and in vivo fertility of buffalo semen. Semen was collected at weekly intervals for a period of three weeks (replicates) from 6 Nili Ravi buffalo bulls (2 ejaculates/bull/replicate) and diluted with TCA extender (50 × 106 motile spermatozoa ml-1 ) containing 5%, 10%, 15% and 20% PEY or 20% CEY (control) and cryopreserved...
June 23, 2018: Theriogenology
Meenakshi Pradhan, Aruna Pal, A K Samanta, Samiddha Banerjee, R Samanta
Cytochrome B is an important polypeptide of the mitochondria helpful in energy metabolism through oxidative phosphorylation. Cytochrome B plays an immense role in the reproduction of animals and due to its mutation prone nature, it can affect the basic physiology of animals. Cytochrome B affects reproductive system in males and equally plays an important role in transferring and providing energy in the development of the embryo, zygote, and oocytes precisely in females. The present study was conducted on Ghungroo pig to study their molecular and reproductive traits and the effect of the cytochrome B gene in the female reproduction of the Ghungroo pig...
June 23, 2018: Theriogenology
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