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Theriogenology

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https://www.readbyqxmd.com/read/28735105/inherent-inferior-quality-of-follicular-fluid-in-repeat-breeder-heifers-as-evidenced-by-low-rates-of-in%C3%A2-vitro-production-of-bovine-embryos
#1
M Kafi, M Azari, O Chashnigir, S Gharibzadeh, Z Aghabozorgi, A Asaadi, M R Divar
The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0...
July 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28734228/effect-of-education-on-ability-of-ai-professionals-and-herd-owner-inseminators-to-detect-cows-not-in-oestrus-and-its-relation-with-progesterone-concentration-on-day-of-re-insemination
#2
K Vartia, J Taponen, J Heikkinen, H Lindeberg
The effect of training background of persons performing artificial insemination (AI) (herd-owner inseminators (OWNER), AI technicians (AI-T), and fertility consultants (FC)) on pregnancy rate and their ability to detect cows not in oestrus were studied. A total of 1584 re-AI occasions on 754 dairy farms were included. Milk samples for progesterone (P4) analysis in all cases were collected, as were data on the herd, previous breeding attempts, oestrous signs, uterine tone, slipperiness of cervix, and co-operation of the cow...
July 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28735106/color-doppler-signals-of-blood-flow-in-the-corpus-luteum-and-vascular-perfusion-index-for-ovarian-and-uterine-arteries-during-expansion-of-the-allantochorion-in-bos-taurus-heifers
#3
F L V Pinaffi, E R Araujo, L A Silva, O J Ginther
Hemodynamics of the CL and each main uterine artery during expansion of the allantochorion from the ipsilateral side (CL side) to the contralateral side were studied in heifers (n = 8 nonbred, 9 pregnant). Progesterone concentration, vascular perfusion index for each uterine artery (by spectral ultrasonography), extent of blood flow in the CL (percentage of CL tissue with color-Doppler signals of blood flow), and intrauterine location of the expanding allantochorion (by gray-scale ultrasonic imaging) were determined daily from Days 14-60 (Day 0 = ovulation)...
July 12, 2017: Theriogenology
https://www.readbyqxmd.com/read/28719823/supplementation-with-dietary-linseed-oil-during-peri-puberty-stimulates-steroidogenesis-and-testis-development-in-rams
#4
REVIEW
Wanhong Li, Defu Tang, Fadi Li, Huaqin Tian, Xiangpeng Yue, Fei Li, Xiuxiu Weng, Wu Sun, Weimin Wang, Futao Mo
Omega-3 polyunsaturated fatty acids (ω-3 PUFAs), such as α-linolenic acid (ALA), eicosapentaenoic acid, and docosahexaenoic acid, are involved in male reproductive function. In this study, we investigated the effects of linseed oil (LO) as a source of ALA on the steroidogenesis and changes of testicular histology in rams. Sixteen 3-month old rams during peri-puberty were randomly assigned into two groups. Eight rams were assigned as the control group, and the other received LO (4% dry matter of total diet) as the LO treatment group...
July 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28716338/changing-innovation-into-a-registered-product-from-concept-to-regulatory-approval
#5
Linda Rhodes
Innovation in animal health pharmaceuticals is important to address unmet and underserved medical needs, and often comes from products initially developed for human medicine. The purpose of the review is to help readers understand how breakthroughs from human biotechnology may be developed for use in veterinary medicine, while understanding the key drivers to success, the difficulties of regulatory approval, and the realistic risks and rewards of developing applications for animals. The types of human drugs which may be useful for veterinary applications are reviewed, including examples...
July 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#6
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708511/fsh-receptor-binding-inhibitor-influences-estrogen-production-receptor-expression-and-signal-pathway-during-in%C3%A2-vitro-maturation-of-sheep-cocs
#7
Gong Zhuandi, Liang Haoqin, Deng Yingying, Lai Luju, Wei Suocheng, Zhaxi Yingpai, Li You
Follicle-stimulating hormone (FSH) promotes secretion of follicle fluid and follicle development. FSH acts via cognate FSH receptor (FSHR). It remains unknown whether the supplement of FSH-receptor binding inhibitor (FRBI) into the in vitro maturation (IVM)medium influence the estrogen receptor expression and signal pathway of oocytes in sheep. The present study aimed to investigate FRBI effects on inositol trisphosphate (IP3) of oocytes and protein kinase A (PKA) of sheep granulosa cells, further to elucidate the signal pathway of FRBI effects...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708510/changes-in-localization-and-density-of-cd63-positive-exosome-like-substances-in-the-hen-oviduct-with-artificial-insemination-and-their-effect-on-sperm-viability
#8
A Huang, N Isobe, Y Yoshimura
Avian sperm are stored in the sperm storage tubules (SSTs) of the hen oviduct for a prolonged period. However, the precise mechanisms by which sperm are kept alive in the SSTs are still not fully understood. The aim of this study was to determine whether exosomes are secreted by SST cells and play a role in the survival of sperm. Utero-vaginal junction (UVJ) tissue from approximately 50 wk old White Leghorn hens was collected before (control group) and after intravaginal insemination with seminal plasma (SP group) or semen (AI group)...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708486/improved-quality-of-porcine-embryos-cultured-with-hyaluronan-due-to-the-modification-of-the-mitochondrial-membrane-potential-and-reactive-oxygen-species-level
#9
Marek Romek, Barbara Gajda, Ewa Krzysztofowicz, Marcin Kucia, Agnieszka Uzarowska, Zdzislaw Smorag
Although considerable progress has been made in pig embryo culture systems, the developmental competence and quality of the produced embryos are still lower than their in vivo-derived counterparts. Because hyaluronan (HA) regulates various cellular processes and possesses antioxidant properties, this glycosaminoglycan seems to be a promising supplement in culture media. However, until now, its beneficial influence on in vitro pig embryo development has been debatable. Hence, we aimed to investigate the effect of 0...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28683952/identification-and-profile-of-micrornas-in-xiang-pig-testes-in-four-different-ages-detected-by-solexa-sequencing
#10
Zhi-Yu Luo, Xin-Lan Dai, Xue-Qin Ran, Yong-Xiu Cen, Xi Niu, Shi-Hui Huang, Jia-Fu Wang
To further understand the role of microRNA (miRNA) during testicular development, we constructed four small RNA libraries from the testes of the Chinese indigenous Xiang pig at four different ages, which were sequenced using high-throughput Solexa deep sequencing methods. It yielded over 23 million high-quality reads and 1,342,579 unique sequences. At two and three months of age, the proportion which represented miRNAs was the most abundant class of small RNAs, but it was gradually replaced by the category that represented piRNAs in adult testes...
June 28, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708508/developmental-changes-in-the-histological-structure-of-the-testes-and-testosterone-profiles-in-male-guinea-fowls-numida-meleagris
#11
Iddriss I Abdul-Rahman, Frederick Y Obese, Ian A Jeffcoate
Owing to the paucity of information on the reproductive biology of guinea fowls, a study involving a total of 66 males was conducted, and documented the developmental changes in histological structure of the testes of guinea cocks from hatching until adulthood. Changes in testosterone synthesis during sexual development were also determined. Age-related changes were analysed using univariate analysis for completely randomised design and means separated using Tukey's test/Kruskal-Wallis test and medians separated by Mann-Whitney U test...
June 23, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708507/effects-of-diluents-and-plasma-on-honey-bee-apis-mellifera-l-drone-frozen-thawed-semen-fertility
#12
REVIEW
Aziz Gül, Nuray Şahinler, Ali G Onal, Brandon K Hopkins, Walter S Sheppard
Cryopreservation is an advanced method used to protect germplasm in liquid nitrogen. Honey bees are of special interest to protect because of their pollination activity and critical role in agriculture. There has been important progress in the cryopreservation of honey bee germplasm in recent years, leading to practical recovery of genetic material for breeding purposes following freezing. However, there remains room for improvement and the goal of the present study was to evaluate the effect of different "extenders" added post-thaw on the fertilization rate of cryopreserved honey bee semen...
June 23, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708521/flow-cytometric-enrichment-of-pacific-bluefin-tuna-type-a-spermatogonia-based-on-light-scattering-properties
#13
REVIEW
Kensuke Ichida, Kazuyoshi Kise, Tetsuro Morita, Ryosuke Yazawa, Yutaka Takeuchi, Goro Yoshizaki
We previously established surrogate broodstock in which the donor germ cells transplanted into the peritoneal cavities of xenogeneic recipients were capable of developing into functional eggs and sperm in teleost fish. In this transplantation system, only the undifferentiated germ cells such as type A spermatogonia (ASG) or a portion of the ASG population were capable of being incorporated into the genital ridges of the recipients and undergo gametogenesis. Therefore, the use of enriched ASGs can be expected to achieve efficient donor-cell incorporation...
June 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708517/factors-affecting-the-reproductive-performance-of-bitches-a-prospective-cohort-study-involving-1203-inseminations-with-fresh-and-frozen-semen
#14
F K Hollinshead, D W Hanlon
The aim of this prospective cohort study was to utilize multivariable statistical methods to identify factors that significantly affected whelping rate, litter size and gestation length in a large population of bitches of many different breeds, presented for routine breeding management. In addition, we aimed to determine the incidence of dystocia and the proportion of bitches undergoing a caesarean section procedure. A total of 1146 individual bitches representing 84 different breeds contributed 1203 inseminations over the 9 year (2007-2015) study period...
June 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708515/stage-specific-expression-of-sal-like-protein-4-in-boar-testicular-germ-cells
#15
Hyun-Jung Park, Ran Lee, Won-Young Lee, Jin-Hoi Kim, Jeong Tae Do, Chankyu Park, Hyuk Song
Spermatogenesis, the complex process of sperm cell development including mitotic cell division and meiosis, relies on spermatogonial stem cells (SSCs). While markers for developing germ cells have been well investigated in mice, developmental stage-specific markers of germ cells in domestic animals have not been identified. Sal-like protein 4 (SALL4) is known as a putative marker for undifferentiated spermatogonia in rodents; however, its expression in domestic animals has not been investigated. The objective of this study was to characterize the expression of SALL4 in the developmental stages of boar testes and SSCs...
June 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708514/feeding-rosemary-leaves-powder-ameliorates-rooster-age-related-subfertility
#16
Seyyed Mohsen Borghei-Rad, Saeed Zeinoaldini, Mahdi Zhandi, Hossein Moravej, Mahdi Ansari
Having a high proportion of polyunsaturated fatty acids avian spermatozoa predispose to lipoperoxidation which results in fertility reduction. In the current study, rosemary leaves powder (RLP) was fed to senescent breeder roosters to improve their reproductive performance. Twenty four 70-week-old roosters were randomly divided into four groups and received following treatments including 0 (RLP-0), 2.5 (RLP-2.5), 5 (RLP-5) or 7.5 (RLP-7.5) g of RLP/kg of diet for eight consecutive weeks. Semen characteristics were evaluated weekly...
June 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708513/interaction-of-extender-composition-and-freezing-method-for-effective-semen-cryopreservation-in-the-north-american-river-otter-lontra-canadensis
#17
Helen L Bateman, William F Swanson
Semen cryopreservation and storage in genome resource banks (GRBs), in combination with artificial insemination (AI), could be invaluable for genetic management and conservation of endangered otter species. For any applied conservation benefit, effective methods for otter sperm processing and cryopreservation first must be established. In this study, our objective was to develop an effective semen cryopreservation method for the North American river otter, evaluating the effect of extender composition (i.e...
June 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708519/relationships-between-uterine-health-and-metabolism-in-dairy-cows-with-different-dry-period-lengths
#18
J Chen, N M Soede, G J Remmelink, R M Bruckmaier, B Kemp, A T M van Knegsel
The first objective of this study was to evaluate effects of dry period (DP) length and dietary energy source on ovarian activity, uterine health status, pregnancy rate, and days open in dairy cows in the second subsequent lactation after implementation of DP length and dietary treatments. The second objective was to determine relationships of uterine health status with ovarian activity, milk yield, energy balance (EB), and metabolic status in dairy cows. Holstein-Friesian dairy cows (n = 167) were assigned randomly to 1 of 3 DP lengths (0-, 30-, or 60-d) and 1 of 2 early lactation diets (glucogenic or lipogenic diet) for 2 subsequent lactations...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708516/peroxisome-proliferator-activated-receptor-ppar-isoforms-are-differentially-expressed-in-peri-implantation-porcine-conceptuses
#19
Agnieszka Blitek, Magdalena Szymanska
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand-dependent transcription factors. PPARs are critical regulators of glucose homeostasis and lipid metabolism, and affect cell proliferation and differentiation. In the current study, we examined (1) the profiles of PPARA, PPARD, and PPARG mRNA expression and DNA binding activity in porcine conceptuses collected on Days 10-11 (spherical and tubular conceptuses), 11-12 (filamentous conceptuses), 13-14, and 15-16 (elongated conceptuses) of pregnancy, (2) the presence of PPARA, PPARD, and PPARG proteins in Days 10, 12, and 15 conceptuses...
June 13, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708512/insulin-during-in%C3%A2-vitro-oocyte-maturation-has-an-impact-on-development-mitochondria-and-cytoskeleton-in-bovine-day-8-blastocysts
#20
Denise Laskowski, Renée Båge, Patrice Humblot, Göran Andersson, Marc-André Sirard, Ylva Sjunnesson
Insulin is a key metabolic hormone that controls energy homeostasis in the body, including playing a specific role in regulating reproductive functions. Conditions associated with hyperinsulinemia can lower developmental rates in bovine in vitro embryo production and are linked to decreased fertility in humans, as in cases of obesity or type 2 diabetes. Embryo quality is important for fertility outcome and it can be assessed by choosing scoring standards for various characteristics, such as developmental stage, quality grade, cell number, mitochondrial pattern or actin cytoskeleton structure...
June 13, 2017: Theriogenology
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