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Ping Chun Wu, Yin-Hung Lin, Lei Fang Tsai, Ming Hung Chen, Pei-Lung Chen, Shun-Chung Pai
BACKGROUND: ABO subtypes are characterized by the alteration of antigens present and their expression levels on red blood cells and many are linked to genetic changes in the ABO gene. Weakened expression of antigens should be identified to prevent transfusion reactions or ABO-incompatible transplantations. Genotyping can be applied to identify subtypes to complement serologic testing. Next-generation sequencing (NGS) has shown to provide sensitive and accurate genotyping results as well as valuable cis/trans information...
May 16, 2018: Transfusion
Donald M Mock, Demet Nalbant, Svetlana V Kyosseva, Robert L Schmidt, Guohua An, Nell I Matthews, Alexander P J Vlaar, Robin van Bruggen, Dirk de Korte, Ronald G Strauss, José A Cancelas, Robert S Franco, Peter Veng-Pedersen, John A Widness
The current reference method in the United States for measuring in vivo population red blood cell (RBC) kinetics utilizes chromium-51 (51 Cr) RBC labeling for determining RBC volume, 24-hour posttransfusion RBC recovery, and long-term RBC survival. Here we provide evidence supporting adoption of a method for kinetics that uses the biotin-labeled RBCs (BioRBCs) as a superior, versatile method for both regulatory and investigational purposes. RBC kinetic analysis using BioRBCs has important methodologic, analytical, and safety advantages over 51 Cr-labeled RBCs...
May 16, 2018: Transfusion
Hamilton C Tsang, Adam Garcia, Robert Scott, David Lancaster, Dianne Geary, Anh-Thu Nguyen, Raina Shankar, Leslie Buchanan, Tho D Pham
BACKGROUND: The ordering process at Stanford Health Care involved twice-daily shipments predicated upon current stock levels from the blood center to the hospital transfusion service. Manual census determination is time consuming and error prone. We aimed to enhance inventory management by developing an informatics platform to streamline the ordering process and reallocate staff productivity. STUDY DESIGN AND METHODS: The general inventory accounts for more than 50 product categories based on characteristics including component, blood type, irradiation status, and cytomegalovirus serology status...
May 16, 2018: Transfusion
Savino Spadaro, Fabio Silvio Taccone, Alberto Fogagnolo, Federico Franchi, Sabino Scolletta, Riccardo Ragazzi, Alberto Fanni, Elisabetta Marangoni, Maurizio Govoni, Roberto Reverberi, Carlo Alberto Volta
BACKGROUND: Red blood cell distribution width (RDW) is a measure of anisocytosis, generally used in the differential diagnosis of anemia. Recently, RDW was associated with increased mortality in critically ill patients. Red blood cell (RBC) transfusions are potential confounders on RDW values interpretation. The aim of this study was to analyze the changes in RDW after RBC transfusion in intensive care unit (ICU) patients. STUDY DESIGN AND METHODS: This was a prospective, observational study including patients admitted to ICU requiring 1 RBC unit...
May 16, 2018: Transfusion
John G Howe, Gary Stack
BACKGROUND: The intrinsic properties of polypeptide blood group antigens that determine their relative immunogenicities are unknown. Because size, composition, charge, dose, and epitope glycosylation affect the immunogenicity of other polypeptides, we examined whether similar properties were related to the immunogenicity of blood group antigens. STUDY DESIGN AND METHODS: Amino acid (AA) sequences of antithetical blood group antigens were searched for N- and O-glycosylation sites...
May 16, 2018: Transfusion
Hilalion Ahn, Jason Tay, Beverley Shea, Brian Hutton, Risa Shorr, Greg A Knoll, Donald William Cameron, Juthaporn Cowan
BACKGROUND: Prophylactic immunoglobulin has been used with varying efficacy to reduce complications in hematopoietic stem cell transplant recipients. STUDY DESIGN AND METHODS: A systematic review and meta-analysis was conducted of randomized controlled trials that assessed clinical outcomes (overall survival, transplant-related mortality, graft-versus-host disease [GVHD], veno-occlusive disease [VOD], interstitial pneumonitis, disease relapse, cytomegalovirus [CMV] infection and disease, non-CMV infection) of immunoglobulin prophylaxis versus placebo in hematopoietic stem cell transplant recipients...
May 16, 2018: Transfusion
Betty J Kipkeu, Melissa L Shyian, Luciana da Silveira Cavalcante, Trang T Duong, Rae S M Yeung, Beth Binnington, Donald R Branch, Jason P Acker, Jelena L Holovati
BACKGROUND: Monocyte monolayer assay (MMA) is a compatibility testing method for evaluating the clinical significance of red blood cell (RBC) alloantibodies. Time-consuming monocyte isolation procedures and requirement for fresh monocytes have limited application of the MMA. The aim of this study was to develop and assess the utility and efficacy of cryopreserved buffy coat (BC)-derived monocytes for MMA application. STUDY DESIGN AND METHODS: Peripheral blood mononuclear cells (PBMNCs) were isolated from BC or peripheral blood (PB) and pooled and BC PBMNCs were cryopreserved...
May 9, 2018: Transfusion
Arne Knörck, Stefanie Marx, Kim S Friedmann, Sylvia Zöphel, Lisa Lieblang, Carmen Hässig, Isabelle Müller, Jan Pilch, Urban Sester, Markus Hoth, Hermann Eichler, Martina Sester, Eva C Schwarz
BACKGROUND: Research with primary human white blood cell (WBC) subpopulations requires high quantity, quality, and functionality of peripheral blood mononuclear cells (PBMCs) as a source to further characterize cellular subpopulations such as T and B lymphocytes, monocytes, or natural killer cells. Apart from buffy coats derived from whole blood, residual blood from preparative hemapheresis kits are used as a source for PBMCs, but knowledge on the yield and functionality of cells from different devices is limited...
May 6, 2018: Transfusion
Xinxin Lin, Marta Torrabadella, Lluís Amat, Susana Gómez, Carmen Azqueta, Mar Sánchez, Daniel Cuadras, Maria José Martínez Lorenzo, José María Brull, Antoni Gayà, Arantxa Cemborain, Cristina Pérez Garcia, JoséLuís Arroyo, Sergi Querol, Maria Dolores Gómez Roig
BACKGROUND: The need for high-cellular-content cord blood units (CBUs) for allogenic transplantation is evident to improve clinical outcomes. In our environment and with current donation programs, very few collected units meet suggested clinical thresholds, making collection programs highly inefficient. To increase the clinical conversion rate, we have assessed factors influencing the cellular content of the cord blood collection and established the estimated fetal weight percentile (EFWp) as a tool to predict which deliveries will obtain higher cellular counts...
May 6, 2018: Transfusion
Nay Win, Malcolm Needs, Nicole Thornton, Robert Webster, Cherry Chang
BACKGROUND: The therapeutic value of the use of intravenous immunoglobulin (IVIG) to correct anemia and thrombocytopenia as a result of immunologic causes (hemolytic disease of the fetus and newborn and fetal or neonatal alloimmune thrombocytopenia) have been well established. Few published papers exist regarding the use of IVIG in adult settings. We report two patients with clinically significant antibodies against high-incidence antigens, who were successfully transfused with incompatible red blood cells (RBCs), in conjunction with IVIG plus steroids and IVIG...
May 6, 2018: Transfusion
Silvia Spoerl, Dagmar Wäscher, Stefanie Nagel, Christian Peschel, Mareike Verbeek, Katharina Götze, Angela M Krackhardt
BACKGROUND: Cell separators are routinely used to collect CD34+ blood stem cells in the context of customized stem cell transplantation procedures. The Spectra Optia (Terumo BCT) is a novel development of the precursor instrument, the Cobe Spectra (Terumo BCT). STUDY DESIGN AND METHODS: In this report, 146 autologous and 42 allogeneic donors undergoing apheresis on the Cobe Spectra using the mononuclear cell (MNC) program 4.7 or on the Spectra Optia using the new continuous mononuclear cell (cMNC) program 11...
May 6, 2018: Transfusion
Antonella Matteocci, Tommaso Mancuso, Federica Pirelli, Tiruneh Hailemariam, Alessandra Moscetti, Katiuscia Castagna, Angela Collaretti, Letizia Rogai, Guido Nespoli, Paola Grammatico, Luca Pierelli
No abstract text is available yet for this article.
May 6, 2018: Transfusion
Markus Eickmann, Ute Gravemann, Wiebke Handke, Frank Tolksdorf, Stefan Reichenberg, Thomas H Müller, Axel Seltsam
BACKGROUND: Ebola virus (EBOV) and Middle East respiratory syndrome coronavirus (MERS-CoV) have been identified as potential threats to blood safety. This study investigated the efficacy of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate EBOV and MERS-CoV in platelet concentrates (PCs) and plasma, respectively. STUDY DESIGN AND METHODS: PCs and plasma were spiked with high titers of cell culture-derived EBOV and MERS-CoV, treated with various light doses of ultraviolet C (UVC; THERAFLEX UV-Platelets) or methylene blue (MB) plus visible light (MB/light; THERAFLEX MB-Plasma), and assessed for residual viral infectivity...
May 6, 2018: Transfusion
Tatiane Aparecida de Paula Vendrame, Flavia Roche Moreira Latini, Rosangela de Medeiros Person, Afonso José Pereira Cortez, Lilian Castilho, Carine Prisco Arnoni
No abstract text is available yet for this article.
May 6, 2018: Transfusion
Aaron S Hess, John R Hess
No abstract text is available yet for this article.
May 6, 2018: Transfusion
Joseph P Connor, Thomas Raife, Joshua E Medow, Bradley D Ehlenfeldt, Kristen Sipsma
BACKGROUND: Electronic decision support has been used to reduce use of red blood cell (RBC) transfusion. With the goal of reducing transfusions, we modified our RBC orders to default to 1 unit. Next, we created a target-based algorithm, the blood utilization calculator or BUC, to calculate a dose in units, based on initial hemoglobin or hematocrit and weight. STUDY DESIGN AND METHODS: RBC orders defaulted to 1 unit in March 2016 and the BUC was implemented in July 2016...
May 1, 2018: Transfusion
Xuan-Duc Nguyen, Torsten J Schulze, Peter Bugert, Stephanie Lauber-Härtl, Monika Schulz-Linkholt, Karen González-Schulze, Angelika Reil, Thomas Dengler, Simon Panzer, Erhard Seifried
BACKGROUND: White blood cell-associated antibodies can lead to transfusion-related acute lung injury (TRALI). Female donors with a history of pregnancies have been identified as a main cause for these antibodies. Male or female donors without a history of pregnancy are considered as safe donors. STUDY DESIGN AND METHODS: Following the identification of two TRALI cases associated with blood products from male donors, we investigated the frequency of granulocyte-specific and human leukocyte antigen (HLA) antibodies in the entire blood donor population using a high throughput automated flow-cytometry-based granulocyte immunofluorescence test (Flow-GIFT)...
April 29, 2018: Transfusion
Luyi Ye, Fengyong Zhao, Qixiu Yang, Jiamin Zhang, Qin Li, Chen Wang, Zhonghui Guo, Ying Yang, Ziyan Zhu
BACKGROUND: Recently, basigin (BSG), which carries OK antigens on red blood cells (RBCs), was reported to be the receptor of the Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRh5). BSG-PfRh5 is the only essential receptor-ligand pair in P. falciparum invasion that is known to date. However, the kind of OK/BSG polymorphism involved in the selection pressure caused by P. falciparum malaria has not been determined. STUDY DESIGN AND METHODS: Blood samples were collected to detect the expression of OK/BSG...
April 29, 2018: Transfusion
Riitta Niittyvuopio, Eeva Juvonen, Jouni Heiskanen, Vesa Lindström, Anne Nihtinen, Leila Sahlstedt, Liisa Volin
BACKGROUND: Steroid-refractory acute graft-versus-host disease (aGVHD) is a serious complication after hematopoietic stem cell transplantation. The long-term outcome of the patients is poor. Various immunosuppressive agents have been proposed as the second-line therapy but none of them has turned out more effective than the others. Extracorporeal photopheresis (ECP) is a treatment option that does not predispose the patients to severe side effects of the immunosuppressive drugs. STUDY DESIGN AND METHODS: We analyzed the treatment results of ECP in 52 patients with steroid-refractory or steroid-dependent aGVHD...
April 29, 2018: Transfusion
Aline Floch, Dominique Gien, Christophe Tournamille, Btissam Chami, Anoosha Habibi, Frédéric Galactéros, Philippe Bierling, Rachid Djoudi, Corinne Pondarré, Thierry Peyrard, France Pirenne
BACKGROUND: Sickle cell disease (SCD) patients undergo multiple red blood cell (RBC) transfusions and are regularly exposed to low-prevalence (LP) antigens specific to individuals of African descent. This study evaluated the prevalence of antibodies against LP antigens in SCD patients and the need to identify these antibodies in everyday practice. STUDY DESIGN AND METHODS: Plasma from 211 SCD patients was tested with RBCs expressing the following LP antigens: RH10 (V), RH20 (VS), RH23 (DW ), RH30 (Goa ), KEL6 (Jsa ), and MNS6 (He)...
April 29, 2018: Transfusion
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