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Vox Sanguinis

S S Lee, E K H Cheung, J N S Leung, C K Lee
BACKGROUND AND OBJECTIVES: Donor screening alone cannot eliminate the risk of transfusion-transmitted HIV infection. Donor deferral according to established criteria is a supplemental strategy, which has focused largely on men who have sex with men (MSM). A study was conducted to determine the compliance of non-MSM donors with such criteria and examine its implications on blood safety. MATERIALS AND METHODS: Chinese donors who have just donated blood at blood donor centres in Hong Kong were recruited...
April 11, 2017: Vox Sanguinis
S Ramirez-Arcos, C Jenkins, W P Sheffield
Although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. Here, we separately spiked thawed cryoprecipitate units with four bacterial strains: Staphylococcus epidermidis, Serratia liquefaciens, Pseudomonas putida and Pseudomonas aeruginosa. No strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. Pathogen inactivation technologies could be explored to mitigate the safety risk posed by extending storage of thawed cryoprecipitate at room temperature...
April 6, 2017: Vox Sanguinis
S M Qadri, D Chen, P Schubert, D V Devine, W P Sheffield
γ-Irradiation of red blood cell (RBC) concentrates prevents transfusion-associated graft-versus-host disease but may diminish RBC quality. Herein, we show that early γ-irradiation (25 Gy) of RBC units and their subsequent storage in SAG-M additive solution altered membrane microvesiculation, supernatant haemoglobin and cytosolic ATP. γ-Irradiation did not influence phosphatidylserine externalization, a marker of erythrocyte apoptotic cell death (eryptosis), in RBC stored for 42 days. However, shorter periods (4-21 days) of storage accentuated eryptosis in γ-irradiated RBC versus untreated RBCs following energy depletion, suggesting that γ-irradiated RBC is primed for stress-induced eryptosis during storage...
April 5, 2017: Vox Sanguinis
M Taha, B Culibrk, M Kalab, P Schubert, Q-L Yi, R Goodrich, S Ramirez-Arcos
BACKGROUND AND OBJECTIVES: Staphylococcus epidermidis forms surface-attached aggregates (biofilms) in platelet concentrates (PCs), which are linked to missed detection during PC screening. This study was aimed at evaluating the efficacy of riboflavin-UV treatment to inactivate S. epidermidis biofilms in buffy coat (BC) PCs. MATERIALS AND METHODS: Biofilm and non-biofilm cells from S. epidermidis ST-10002 and S. epidermidis AZ-66 were individually inoculated into whole blood (WB) units (~10(6) colony-forming units (CFU)/ml) (N = 4-5)...
April 5, 2017: Vox Sanguinis
C R Seed, H Yang, J F Lee
HIV pre-exposure prophylaxis (PrEP) is the use of one or more antiretroviral medications (in combination) to prevent HIV infection. The most commonly used PrEP medication (Truvada(®) , Gilead Sciences, Inc.) acts by inhibiting HIV-1 reverse transcriptase. If someone who is using PrEP unknowingly becomes HIV infected (termed 'PrEP breakthrough infection'), there may be suppressed viral replication resulting in a virus level undetectable by the most sensitive HIV NAT. Failure to seroconvert and seroreversion (loss of previously detectable HIV antibodies) have also both been observed with 2nd, 3rd and 4th generation screening immunoassays, as well as Western blot assays...
March 31, 2017: Vox Sanguinis
Ó Rolfsson, Ó E Sigurjonsson, M Magnusdottir, F Johannsson, G Paglia, S Guðmundsson, A Bordbar, S Palsson, S Brynjólfsson, S Guðmundsson, B Palsson
BACKGROUND AND OBJECTIVES: Metabolomics studies have revealed transition points in metabolic signatures of red cells during storage in SAGM, whose clinical significance is unclear. We set out to investigate whether these transition points occur independent of storage media and define differences in the metabolism of red cells in additive solutions. MATERIALS AND METHODS: Red cell concentrates were stored in SAGM, AS-1, AS-3 or PAGGSM, and sampled fourteen times spanning Day 1-46...
March 31, 2017: Vox Sanguinis
X Hong, S Chen, Y Ying, Y Liu, X Xu, J He, F Zhu
BACKGROUND AND OBJECTIVES: Human platelet alloantigen (HPA) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for HPA-1 to -28bw systems was established using multiplex PCR-SBT and the frequencies of genotypes and alleles of HPA-1 to -28bw systems in the Zhejiang Han population were analysed. MATERIALS AND METHODS: The specific primers were designed according to the nucleotide sequences of HPA-1 to 28bw systems which are located in ITGB3, GP1BA, ITGA2B, ITGA2, GP1BB and CD109, respectively...
March 31, 2017: Vox Sanguinis
E Hussein, J Teruya
BACKGROUND AND OBJECTIVES: Thrombotic thrombocytopenic purpura (TTP) is caused by the decrease of ADAMTS13, leading to the accumulation of ultra large von Willebrand factor (ULVWF). It was proposed that the distribution of blood group O among TTP patients may be potentially lower than expected because of the lower levels of VWF. The aim of this study was to explore the relationship between various blood groups and the clinical outcome in TTP. MATERIALS AND METHODS: Thirty-three patients with TTP with severe ADAMTS13 deficiency were studied...
March 31, 2017: Vox Sanguinis
L Noens, Ma D Vilariño, A Megalou, H Qureshi
BACKGROUND AND OBJECTIVES: Methylene blue is a phenothiazine dye, which in combination with visible light has virucidal and bactericidal properties, disrupting the replication of a broad range of enveloped viruses and some non-enveloped viruses. The study objective was to collect data on adverse reactions occurring with methylene blue plasma administered in a routine clinical practice environment and document their characteristics and severity. MATERIALS AND METHODS: This was an open label, multicentre, non-controlled, non-randomized, non-interventional study...
March 26, 2017: Vox Sanguinis
M Germain, Y Grégoire, R R Vassallo, J P Acker, R Cardigan, D de Korte, D O Irving, S Bégué
BACKGROUND AND OBJECTIVES: Blood operators routinely monitor the pH of apheresis platelets as a marker of the so-called storage lesion, which can result from manufacturing problems. It is also suspected that some donor characteristics can increase the risk of poor platelet storage. To explore this hypothesis, we analysed a large, multinational data set of quality control (QC) pH test results on apheresis platelets. MATERIALS AND METHODS: For the period between September 2011 and August 2014, seven blood operators in Canada, the USA, the Netherlands, the United Kingdom, France and Australia provided pH QC test results and donor characteristics on a total of 21,671 apheresis platelets...
March 23, 2017: Vox Sanguinis
M Abonnenc, B Pesse, J-D Tissot, S Barelli, N Lion
BACKGROUND AND OBJECTIVES: Autologous haematopoietic progenitor cell (HPC) is a prerequisite for high-dose chemotherapy in treatment of several haematologic and non-haematologic malignancies. HPCs are collected by apheresis and cryopreserved until infusion. Postinfusion adverse events have been in part related to the dimethyl sulphoxide (DMSO) used as cryoprotectant. The aim was to evaluate (i) an automated sequential washing procedure for DMSO removal in thawed HPC grafts and (ii) washing solutions in replacement of hydroxyethyl starch (HES)-based solutions...
March 23, 2017: Vox Sanguinis
R Celli, W Schulz, J E Hendrickson, C A Tormey
We hypothesized that diagnoses may be associated with alloantibody 'responder' status and examined associations between disease states and alloimmunization. Patients with ≥1 alloantibody and non-alloimmunized controls were analysed. Pearson's coefficients were calculated to determine associations between alloimmunization and diseases; significant correlations were selected to construct a network. Inflammatory disorders and diseases requiring chronic transfusion support were associated with responder status...
March 23, 2017: Vox Sanguinis
D C Marks, P F van der Meer
BACKGROUND: Serum eye drops (SEDs) are used to treat dry eye syndrome and non-healing corneal lesions when other treatments fail. Despite many clinical studies demonstrating the efficacy of both autologous and allogeneic SEDs, there is no internationally harmonized method for producing SEDs. MATERIALS AND METHODS: A 40-question survey requesting information regarding donor selection, blood collection and processing, infectious disease screening, shelf life and regulatory requirements for the production of autologous and allogeneic SEDs was developed by the Biomedical Excellence for Safer Transfusion Collaborative...
March 23, 2017: Vox Sanguinis
R L Barty, M Pai, Y Liu, D M Arnold, R J Cook, M P Zeller, N M Heddle
BACKGROUND: There have been recurrent shortages of group O blood due to insufficient inventory and use of group O blood in ABO non-identical recipients. We performed a 12-year retrospective study to determine utilization of group O Rh-positive and Rh-negative red blood cells (RBCs) by recipient ABO group. Reasons for transfusing group O blood to ABO non-identical recipients were also assessed. METHODS: Utilization data from all group O Rh-positive and Rh-negative RBCs transfused at three academic hospitals between April 2002 and March 2014 were included...
March 20, 2017: Vox Sanguinis
R R Sharma, M Lozano, M Fearon, M Bigham, R Djoudi, P Gallian, G Woimant, C Lee, J N S Leung, W C Tsoi, N Marwaha, S Sachdev, K Tadokoro, Y Tani, H Matsukura, N Shantseva, E Zhiburt, S Hindawi, J Chay, T Huang, D Teo, N Moleli, S Oyonarte, S B A Jayasekara, A Bokhorst, P van den Burg, P Hewitt, C Bianco, D Kessler
No abstract text is available yet for this article.
March 20, 2017: Vox Sanguinis
R R Sharma, M Lozano, M Fearon, M Bigham, R Djoudi, P Gallian, G Woimant, C Lee, J N S Leung, W C Tsoi, N Marwaha, S Sachdev, K Tadokoro, Y Tani, H Matsukura, N Shantseva, E Zhiburt, S Hindawi, J Chay, T Huang, D Teo, N Moleli, S Oyonarte, S B A Jayasekara, A Bokhorst, P van den Burg, P Hewitt, C Bianco, D Kessler
No abstract text is available yet for this article.
March 20, 2017: Vox Sanguinis
A Torres-Ortuño, R Cid-Sabatel, J Barbero, M García-Dasí
This article discusses, from a psychological perspective, the life experience of the adult and ageing person with haemophilia, including psychological issues, aspects of his personal and social integration, decision-making, communication and other factors that may affect treatment adherence and quality of life. The aim was to provide haematologists and healthcare staff with knowledge and resources to improve communication and support for adult persons with haemophilia, and raise awareness on psychosocial issues related to quality of life, sexuality and aspects associated with ageing with haemophilia...
March 15, 2017: Vox Sanguinis
C Liu, E Schindler, M LeRoy, K Martin-Bredahl, M Dino, K Shah, M Dynis, C S Eby, B J Grossman
BACKGROUND: Extracorporeal photopheresis (ECP) is an established treatment for graft-versus-host disease (GVHD). Various haematocrit thresholds have been used to trigger red blood cells transfusion prior to ECP. Moderate-to-severe GVHD is frequently complicated by anaemia; the safety and collection efficiency with a lower haematocrit for ECP is unknown. METHODS: We prospectively enrolled 26 consecutive adult GVHD patients with haematocrits between 25% and 28·9% who received ECP on the CELLEX system...
March 7, 2017: Vox Sanguinis
A L Kreuger, C Caram-Deelder, J Jacobse, J-L Kerkhoffs, J G van der Bom, R A Middelburg
BACKGROUND: Prolonged storage improves availability of platelet products but could also influence safety and efficacy. This systematic review and meta-analyses summarize and quantify the evidence of the effect of storage time of transfused platelets on clinical outcomes. METHODS: A systematic search in seven databases was performed up to February 2016. All studies reporting storage time of platelet products and clinical outcomes were included. To quantify heterogeneity, I² was calculated, and to assess publication bias, funnel plots were constructed...
March 7, 2017: Vox Sanguinis
E K van de Weerdt, A L Peters, E J Goudswaard, J M Binnekade, K P van Lienden, B J Biemond, A P J Vlaar
BACKGROUND: Correction of coagulopathy prior to central venous catheter (CVC) placement is advocated by guidelines, while retrospective studies support restrictive use of transfusion products. STUDY DESIGN AND METHODS: We conducted a mixed vignette and questionnaire web survey to investigate current practice and preferences for CVC placement. Clinical vignettes were used to quantify the tendency to administer platelet concentrate. A positive ß-coefficient is in favour of administering platelet concentrate...
March 5, 2017: Vox Sanguinis
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