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Kobe Journal of Medical Sciences

Qingyun, Yan Ma, Toshiaki Kato, Tomoyuki Furuyashiki
In Schizosaccharomyces pombe, Ssp2, an ortholog of AMP-activated protein kinase (AMPK), is critical for cell growth at restrictive temperatures and under glucose depletion as well as sexual differentiation under nitrogen depletion. To identify genes genetically related to Ssp2, we performed a genetic screening to search for the genes whose overexpression rescued the growth defects in Δssp2 cells at restrictive temperatures, and identified 35 cosmids as multicopy suppressor genes. In Southern blot analyses, 22 out of these cosmids were hybridized to an ssp2+ probe...
August 3, 2016: Kobe Journal of Medical Sciences
Endah Wulandari, Sri Widia A Jusman, Yefta Moenadjat, Ahmad A Jusuf, Mohamad Sadikin
BACKGROUND: Wound heals itself spontaneously as physiological process. However, in some individuals, small wounds such as parenteral injections or body piercings may cause increased expression of collagen synthesis. The condition is known as keloid. Histopathology of keloid demonstrates extensive tissue proliferation that extends beyond the margin of primary wound. As a result, it develops uncontrolled or excessive fibrogenesis and tremendous source of collagen that still causes clinical problems until now...
July 25, 2016: Kobe Journal of Medical Sciences
Shungo Furudoi, Takashi Yoshii, Takahide Komori
We describe a patient with oculomotor nerve palsy due to secondary orbital infiltration from the primary malignant lymphoma of the cheek. The patient was a 78-year-old female who had non-Hodgkin lymphoma (diffuse large B cell lymphoma [DLBCL]) of the cheek. The patient received chemotherapy and local radiation therapy. The combined treatment brought about complete remission. About 6 months after the last treatment the patient began to have left blepharoptosis and impaired vision. Findings from ophthalmological and neurosurgical examinations suggested no intraorbital or intracranial lesions...
July 25, 2016: Kobe Journal of Medical Sciences
Ali Bülent Baz, Serdar Akalin, Ömer Faruk Kiliçaslan, Bogaçhan Tokatman, Hasan Arik, Fatih Duygun
Eighty-seven osteoporotic vertebral fractures of 82 patients were treated with balloon kyphoplasty. Preoperative, postoperative and follow-up outcomes of functional impairment, pain scores and vertebral height restorations of the patients were recorded and evaluated statistically. Seventy-two of the patients were female (87.8%) and 10 (12.2%) were male. Mean age of all patients were 66.4 years. Preoperative mean Anterior Vertebral Body Height of 48.20±13.94 % and Middle Vertebral Body Height of 59.40±14.26 % were recorded as 79...
July 14, 2016: Kobe Journal of Medical Sciences
Satoshi Nagamata, Yasuhiko Ebina, Yumika Yamano, Takeo Miyamoto, Mitsuhiro Nishijima, Hideto Yamada
Uterine leiomyosarcoma (ULMS) is an aggressive tumor associated with high rates of progression, recurrence, and mortality. Pazopanib is the only approved molecular targeted drug for advanced soft tissue sarcoma, and it has been proven to prolong progression-free survival relative to placebo. We herein report a case of ULMS with multiple lung metastases treated with pazopanib, which led to sustained disease control for 44 weeks. A 53-year-old woman was referred to our hospital due to massive uterine bleeding from a uterine corpus tumor mass...
July 5, 2016: Kobe Journal of Medical Sciences
Nur Arfian, Khusnul Muflikhah, Sri Kadarsih Soeyono, Dwi Cahyani Ratna Sari, Untung Tranggono, Nungki Anggorowati, Muhammad Mansyur Romi
Kidney fibrosis is the common final pathway of chronic kidney diseases (CKD). It is characterized by myofibroblast formation, inflammation, and epithelial architecture damage. Vitamin D is known as a renoprotective agent, although the precise mechanism is not well understood. This study aimed to elucidate the effect of vitamin D in fibroblast expansion, inflammation, and apoptosis in kidney fibrosis. We performed unilateral ureteral obstruction (UUO) in male Swiss-Webster background mice (3 months, 30-40 grams) to induce kidney fibrosis...
July 5, 2016: Kobe Journal of Medical Sciences
Ryuichi Totsuka, Takaaki Kondo, Shigeki Matsubara, Midori Hirai, Yoichi Kurebayashi
VLA-1 (very late antigen-1) is implicated in recruitment, retention and activation of leukocytes and its blockade has been referred as a potential target of new drug discovery to address unmet medical needs in inflammatory disease area. In the present study, we investigate the effects of an anti-murine CD49a (integrin α subunit of VLA-1) monoclonal antibody (Ha31/8) on various experimental models of inflammatory diseases in mice. Pretreatment with Ha31/8 at an intraperitoneal dose of 250 µg significantly (P<0...
July 5, 2016: Kobe Journal of Medical Sciences
Irwanto, Nancy Margarita Rehatta, Sri Hartini, Satoshi Takada
BACKGROUND: Sleep problems are associated with problems of cognitive functioning, learning, attention and school performance. It has been found that sleep problems are highly prevalent in children with Autistic spectrum disorders (ASD), with rates ranging from 40% to 80%. We aimed to identify the prevalence of sleep problems on children with ASD in Indonesia and Japan. METHODS: A cross-sectional study was conducted in Surabaya, Indonesia and Kobe, Japan. Children aged 4 -10 years old were enrolled using stratified cluster sampling...
July 4, 2016: Kobe Journal of Medical Sciences
Keisuke Arai, Kaori Kuramitsu, Takumi Fukumoto, Masahiro Kido, Atsushi Takebe, Motofumi Tanaka, Hisoka Kinoshita, Tetsuo Ajiki, Hirochika Toyama, Sadaki Asari, Tadahiro Goto, Yonson Ku
There are few descriptions of severe thrombocytopenia during the early postoperative period after liver transplantation, and these have not been fully documented in the literature. Here, we report a case of drug-induced thrombocytopenia requiring transfusion of blood products after living donor liver transplantation. We determined that this was not caused by the interferon-free anti-viral therapy but by tacrolimus A 61-year-old woman with hepatitis C-related cirrhosis and hepatorenal syndrome underwent living donor liver transplantation using a left lobe graft from her son...
June 16, 2016: Kobe Journal of Medical Sciences
Issei Nagura, Takako Kanatani, Masatoshi Sumi, Takeshi Kokubu, Masahiro Kurosaka
We report a case of recurrence of enchondroma in a middle finger after curettage and back-filling with calcium phosphate bone cement (CPC). The radiograph showed a lytic lesion around the CPC filling which showed no signs of absorption after 12 years. The tumor was curated easily, however, a steel bar was needed to remove the CPC mass in a carefully manner not to break the cortex. CPC has an advantage of immediate biomechanical stability, on the other hand, a disadvantage of being unabsorbed inside of bone...
June 16, 2016: Kobe Journal of Medical Sciences
Kaku Matsugi, Tetsuya Hosooka, Kazuhiro Nomura, Wataru Ogawa
Thrombospondin 1 (TSP-1) is abundantly expressed in visceral adipose tissue and this expression is up-regulated in obese humans and rodents. Recent studies showed that genetic deletion of TSP-1 protects mice from diet-induced insulin resistance. However, the molecular mechanism is largely unknown. In this study, we examined the effect of recombinant TSP-1 on insulin signaling in cultured cells from insulin sensitive tissues to investigate whether TSP-1 could act as an adipokine. Here we show that treatment with recombinant TSP-1 suppressed insulin signaling in cultured muscle cells, which was accompanied by the activation of stress signaling such as JNK, p38, and IKK...
June 16, 2016: Kobe Journal of Medical Sciences
Widya Wasityastuti, Yoshihiko Yano, Dewiyani Indah Widasari, Laura Navika Yamani, Neneng Ratnasari, Didik Setyo Heriyanto, Rina Okada, Toshihito Tanahashi, Yoshiki Murakami, Takeshi Azuma, Yoshitake Hayashi
A nucleos(t)ide analog (NA) is the common antiviral drug available for directly treating hepatitis B virus (HBV) infection. However, its application has led to the emergence of NA-resistant mutations mostly in a conserved region of the reverse transcriptase domain of HBV polymerase. Harboring NA-resistant mutations decreases drug effectiveness and increases the frequency of end-stage liver disease. The invention of next-generation sequencing that can generate thousands of sequences from viral complex mixtures provides opportunities to detect minor changes and early viral evolution under drug stress...
June 16, 2016: Kobe Journal of Medical Sciences
Yukihiro Okada, Miho Murata, Tatsushi Toda
OBJECTIVES: The effects of levodopa on articulatory dysfunction in patients with Parkinson's disease remain inconclusive. This study aimed to investigate the effects of levodopa on isolated vowel articulation and motor performance in patients with moderate to severe Parkinson's disease, excluding speech fluctuations caused by dyskinesia. METHODS: 21 patients (14 males and 7 females) and 21 age- and sex- matched healthy subjects were enrolled. Together with motor assessment, the patients phonated five Japanese isolated vowels (/a/, /i/, /u/, /e/, and /o/) 20 times before and 1 h after levodopa treatment...
April 27, 2016: Kobe Journal of Medical Sciences
Tatsuya Tomonaga, Shiba Kumar Rai, Shoji Uga
The use of nucleic acid staining with a fluorochrome dye to differentiate viable and dead (heat-killed) Cryptosporidium oocysts was assessed. The specificities (percentage of unstained viable oocysts) and sensitivities (percentage of stained dead oocysts) of the seven tested dyes (SYTO-17® and SYTO-59® to 64®) ranged from 65 to 76% (average 71%) and 83 to 95% (average 91%), respectively. SYTO-59 and SYTO-17 imparted greater color (4+) intensity than the other dyes (2+ or less). Of these two dyes, SYTO-17 exhibited more brightness and slower discoloration and was selected for use in further experiments...
April 4, 2016: Kobe Journal of Medical Sciences
Yumiko Takao, Yutaka Takaoka, Aki Sugano, Hitoaki Sato, Yasushi Motoyama, Mika Ohta, Takashi Nishimoto, Satoshi Mizobuchi
Shakuyaku-kanzo-to (Shao-Yao-Gan-Cao-Tang) is a Kampo medicine, which is known to be effective against muscle cramps as well as crampy pain in the gastrointestinal smooth muscle and skeletal muscle. However, glycyrrhizin in this medicine also causes adverse drug reactions such as hypokalemia, hypertension, and edema. We analyzed the therapeutic efficacy of Shakuyaku-kanzo-to for painful muscle cramps associated with lumbar spinal stenosis and clarified its minimum effective dose. 58 patients with lumbar spinal stenosis and painful muscle cramps were included...
April 4, 2016: Kobe Journal of Medical Sciences
Daisuke Tsugawa, Takumi Fukumoto, Masahiro Kido, Atsushi Takebe, Motofumi Tanaka, Kaori Kuramitsu, Ippei Matsumoto, Tetsuo Ajiki, Tatsuki Koyama, Yonson Ku
Alpha-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are frequently used as tumor markers in hepatocellular carcinoma (HCC). The authors hypothesized different patient populations with varying tumor sizes would influence the predictive power of tumor markers for survival in HCC patients. The authors investigated the influence of tumor size on predictive powers of AFP and DCP. 181 patients underwent hepatectomy for HCC from 2003 to 2008 at Kobe University Hospital. Tumor markers were measured before and at 1 month post-hepatectomy...
March 7, 2016: Kobe Journal of Medical Sciences
Ahmad Aulia Jusuf, Hiroyuki Sakagami, Satoshi Kikkawa, Toshio Terashima
Expression of beta 2 subunit of Ca²+/calmodulin-dependent protein kinase I (CaMKIβ2) of the rat retina during the developmental period and in the adulthood was studied immunohistochemically. The immunoreactivity of CaMKIβ2 was detected in the earliest development of the primordial retina at embryological day (E) 12. The inner neuroblastic layer from which the presumptive ganglion cells are generated showed the ubiquitous CaMKIβ2 immunoreactivity at E15 and persistently expressed at the same level until postnatal day (P) 0 when the inner neuroblastic layer divides into the ganglionic cell layer and the inner plexiform layer...
March 7, 2016: Kobe Journal of Medical Sciences
Ayako Sonoyama, Masatoshi Kanda, Yojiro Ojima, Tomohiko Kizaki, Noriyuki Ohara
Aggressive adult granulosa cell tumor (AGCT) of the ovary remains uncommon. We report a case of aggressive AGCT of the ovary who had rapid recurrence at two months after surgery. A patient was referred for further examination of a pelvic tumor. She underwent total abdominal hysterectomy, bilateral salpingo-oophorectomy, and pelvic lymphadenectomy. In the areas showing a sarcomatoid pattern, the mitotic count were 25/10 HPFs, and the mitoses were most prominent in foci composed of pleomorphic cells with enlarged and bizarre nuclei...
February 17, 2016: Kobe Journal of Medical Sciences
Hiroaki Suzuki, Daiki Takahashi, Kenji Matsuo, Akira Kimoto, Kousuke Matsumoto, Takumi Hasegawa, Masaya Akashi, Takahide Komori
Dental implant treatment is a highly anticipated therapy with widespread use. However, various accidental symptoms and complications have been reported, and we may reluctantly have to remove an implant body out of necessity. This study evaluated 19 cases (21 jaws) that resulted in removal after the enforcement of implant treatment in our hospital from January 2003 to August 2013 and clarified the details of the removals clinically for the purpose of improvement of future treatment results. Implants were removed at a higher frequency in elderly patients, particularly those 70 years of age or older...
February 17, 2016: Kobe Journal of Medical Sciences
Keiichi Hayashida, Yoshihiro Takeda, Hidetoshi Yatake, Motoi Yamaguchi, Kenyu Yamamoto, Tadao Kuwano, Toshizo Katsuda
Bone mineral density (BMD) is affected by lean body mass and body weight to various degrees in the course of aging. The attempt of this study is to determine the optimal time to begin prevention of osteoporosis. In this study, female hospital employees aged 20-59 years were divided into 2 age groups, 20-39 years and 40-59 years based on age at peak BMD, and the relations of total BMD, subtotal BMD and lumbar spine BMD to lean body mass and body weight were examined in both groups. Subtotal BMD was calculated by subtracting head BMD from total BMD along with whole body measurement...
December 18, 2015: Kobe Journal of Medical Sciences
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