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Archives of Microbiology

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https://www.readbyqxmd.com/read/28432382/two-distinct-periplasmic-enzymes-are-responsible-for-tellurite-tellurate-and-selenite-reduction-by-strain-er-te-48-associated-with-the-deep-sea-hydrothermal-vent-tube-worms-at-the-juan-de-fuca-ridge-black-smokers
#1
Chris Maltman, Lynda J Donald, Vladimir Yurkov
Strain ER-Te-48 isolated from a deep-ocean hydrothermal vent tube worm is capable of resisting and reducing extremely high levels of tellurite, tellurate, and selenite, which are used for respiration anaerobically. Tellurite and tellurate reduction is accomplished by a periplasmic enzyme of 215 kDa comprised of 3 subunits (74, 42, and 25 kDa) in a 2:1:1 ratio. The optimum pH and temperature for activity is 8.0 and 35 °C, respectively. Tellurite reduction has a V max of 5.6 µmol/min/mg protein and a K m of 3...
April 21, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28432381/modulation-of-the-multidrug-efflux-pump-emrd-3-from-vibrio-cholerae-by-allium-sativum-extract-and-the-bioactive-agent-allyl-sulfide-plus-synergistic-enhancement-of-antimicrobial-susceptibility-by-a-sativum-extract
#2
Merissa M Bruns, Prathusha Kakarla, Jared T Floyd, Mun Mun Mukherjee, Robert C Ponce, John A Garcia, Indrika Ranaweera, Leslie M Sanford, Alberto J Hernandez, T Mark Willmon, Grace L Tolson, Manuel F Varela
The causative agent of cholera, Vibrio cholerae, is a public health concern. Multidrug-resistant V. cholerae variants may reduce chemotherapeutic efficacies of severe cholera. We previously reported that the multidrug efflux pump EmrD-3 from V. cholerae confers resistance to multiple structurally distinct antimicrobials. Medicinal plant compounds are potential candidates for EmrD-3 efflux pump modulation. The antibacterial activities of garlic Allium sativum, although poorly understood, predicts that a main bioactive component, allyl sulfide, modulates EmrD-3 efflux...
April 21, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28421249/co-effects-of-pyrene-and-nitrate-on-the-activity-and-abundance-of-soil-denitrifiers-under-anaerobic-condition
#3
Zhi-Feng Zhou, Yan-Hong Yao, Ming-Xia Wang, Xiao-Hu Zuo
It has previously been confirmed that polycyclic aromatic hydrocarbons (PAHs) could be degraded by soil microbes coupling with denitrification, but the relationships among soil denitrifiers, PAHs, and nitrate under obligate anaerobic condition are still unclear. Here, co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers were investigated through a 45-day incubation experiment. Two groups of soil treatments with (N30) and without (N0) nitrate (30 mg kg(-1) dry soil) amendment were conducted, and each group contained three treatments with different pyrene concentrations (0, 30, and 60 mg kg(-1) dry soil denoted as P0, P30, and P60, respectively)...
April 18, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28417144/the-effect-of-reconstruction-works-on-planktonic-bacterial-diversity-of-a-unique-thermal-lake-revealed-by-cultivation-molecular-cloning-and-next-generation-sequencing
#4
Gergely Krett, Attila Szabó, Tamás Felföldi, Károly Márialigeti, Andrea K Borsodi
The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical-chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches...
April 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28396915/different-nitrogen-sources-change-the-transcriptome-of-welan-gum-producing-strain-sphingomonas-sp-atcc-31555
#5
Xiaopeng Xu, Zuoming Nie, Zhiyong Zheng, Li Zhu, Hongtao Zhang, Xiaobei Zhan
To reveal effects of different nitrogen sources on the expressions and functions of genes in Sphingomonas sp. ATCC 31555, it was cultivated in medium containing inorganic nitrogen (IN), organic nitrogen (ON), or inorganic-organic combined nitrogen (CN). Welan gum production and bacterial biomass were determined, and RNA sequencing (RNA-seq) was performed. Differentially expressed genes (DEGs) between the different ATCC 31555 groups were identified, and their functions were analyzed. Welan gum production and bacterial biomass were significantly higher in the ON and CN groups compared with those in the IN group...
April 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28391506/inactivation-of-genes-involved-in-base-excision-repair-of-corynebacterium-glutamicum-and-survival-of-the-mutants-in-presence-of-various-mutagens
#6
Fabiola Kautzmann, Josef Altenbuchner
Base Excision Repair (BER) is considered as the most active DNA repair pathway in vivo, which is initiated by recognition of the nucleotide lesions and excision of the damaged DNA base. The genome of Corynebacterium glutamicum ATCC 13032 contains various DNA glycosylases encoding genes (ung, fpg/mutM, tagI, alkA, mutY), two AP-endonuclease encoding genes (nei and nth) and an exonuclease encoding gene xth. To investigate the role of these genes during DNA repair in C. glutamicum, mutants with deletions of one or more genes in BER pathway were created...
April 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28391505/bacterial-communities-on-facial-skin-of-teenage-and-elderly-thai-females
#7
Naraporn Somboonna, Alisa Wilantho, Chutika Srisuttiyakorn, Anunchai Assawamakin, Sissades Tongsima
The Human Microbiome Project was first established to understand the roles of human-associated microbes to human health and disease. This study presents preliminary findings of Thai female facial skin microbiome using three pooled samples from groups of skin microbiome profiles, namely (1) healthy and (2) acne-prone young adults (teenage.hea and teenage.acn) and (3) healthy elderly adults (elderly.hea) based on standard dermatological criteria. These samples were sequenced using 454-pyrosequencing targeting 16S rRNA (V3-V4 regions)...
April 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28389815/identification-of-two-regulatory-genes-involved-in-carbomycin-biosynthesis-in-streptomyces-thermotolerans
#8
Jingjing Zhong, Zhili Lu, Jianlu Dai, Weiqing He
Carbomycins are 16-membered macrolide antibiotics produced by Streptomyces thermotolerans ATCC 11416(T). To characterize gene cluster responsible for carbomycin biosynthesis, the draft genome sequences for strain ATCC 11416(T) were obtained, from which the partial carbomycin biosynthetic gene cluster was identified. This gene cluster was approximately 40 kb in length, and encoding 30 ORFs. Two putative transcriptional regulatory genes, acyB2 and cbmR, were inactivated by insertion of the apramycin resistance gene, and the resulting mutants were unable to produce carbomycin, thus confirming the involvement of two regulatory genes in carbomycin biosynthesis...
April 7, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28386666/electrophoretic-profiles-of-lipopolysaccharides-from-rhizobium-strains-nodulating-pisum-sativum-do-not-reflect-phylogenetic-relationships-between-these-strains
#9
Jolanta Kutkowska, Monika Marek-Kozaczuk, Jerzy Wielbo, Marek Wójcik, Teresa Urbanik-Sypniewska
Rhizobia that nodulate peas comprise a heterogeneous group of bacteria. The aim of this study was to investigate the relationship between phylogeny and electrophoretic and hydroxy fatty acid lipopolysaccharide (LPS) profiles of pea microsymbionts. Based on amplified fragment length polymorphism (AFLP) fingerprinting data, the pea microsymbionts were grouped into two clusters distinguished at 58% similarity level. Based on the concatenated 16S rRNA, recA, and atpD housekeeping gene data, the microsymbionts appeared to be most closely related to Rhizobium leguminosarum biovars viciae and trifolii...
April 6, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28386665/agrobacterium-deltaense-sp-nov-an-endophytic-bacteria-isolated-from-nodule-of-sesbania-cannabina
#10
Jun Yan, Yan Li, Xiao Zeng Han, Wen Feng Chen, Wen Xiu Zou, Zhihong Xie, Meng Li
A Gram-negative, non-spore-forming, aerobic rods, strain YIC4121(T), was isolated from root nodule of Sesbania cannabina grown in Dongying (Yellow River Delta), Shandong Province, PR China. Based on phylogenetic analysis of 16 S rRNA gene sequences, strain YIC4121(T) was assigned to the genus Agrobacterium with 99.7, 99.3, 99.0, 98.8 and 98.7% sequence similarities to Agrobacterium radiobacter LMG140(T), A. pusense NRCPB10(T), A. arsenijevicii KFB 330(T), A. nepotum 39/7(T) and A. larrymoorei ATCC51759(T)...
April 6, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28382474/selection-and-characterization-of-coal-mine-autochthonous-rhizobia-for-the-inoculation-of-herbaceous-legumes
#11
Anabel González Hernández, Ginaini Doin de Moura, Renato Leal Binati, Francisco Xavier Inês Nascimento, Diana Morales Londoño, Ana Carolina Peixoto Mamede, Emanuela Pille da Silva, Rafael Dutra de Armas, Admir José Giachini, Márcio José Rossi, Cláudio Roberto Fonsêca Sousa Soares
Coal open pit mining in the South of Santa Catarina state (Brazil) was inappropriately developed, affecting approximately 6.700 ha. Re-vegetation is an alternative for the recovery of these areas. Furthermore, the use of herbaceous legumes inoculated with nitrogen fixing bacteria is motivated due to the difficulty implementing a vegetation cover in these areas, mainly due to low nutrient availability. Therefore, the aim of this work was to evaluate, among 16 autochthonous rhizobia isolated from the coal mining areas, those with the greatest potential to increase growth of the herbaceous legumes Vicia sativa and Calopogonium mucunoides...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28382473/strains-of-photorhabdus-spp-associated-with-polish-heterorhabditis-isolates-their-molecular-and-phenotypic-characterization-and-symbiont-exchange
#12
Waldemar Kazimierczak, Henryk Skrzypek, Ewa Sajnaga, Marcin Skowronek, Adam Waśko, Anna Kreft
The relationships between six bacterial symbionts of the entomopathogenic nematodes Heterorhabditis bacteriophora and Heterorhabditis megidis from Poland to species and subspecies of the genus Photorhabdus were evaluated. This study was based on phylogenetic analysis of sequence data of five genes: 16S rRNA, gyrB, recA, gltX, and dnaN. The bacteria were also characterized phenotypically by biochemical and physiological tests. Our results have revealed that the Photorhabdus strains isolated from H. megidis belong to P...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28382472/omp31-plays-an-important-role-on-outer-membrane-properties-and-intracellular-survival-of-brucella-melitensis-in-murine-macrophages-and-hela-cells
#13
L Verdiguel-Fernández, R Oropeza-Navarro, Francisco J Basurto-Alcántara, A Castañeda-Ramírez, Antonio Verdugo-Rodríguez
Brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. Among the six recognized species of the genus Brucella, Brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. It causes significant losses in livestock production as a result of abortions, metritis, infertility, and birth of weak animals...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28382471/two-new-species-of-the-genus-streptomyces-streptomyces-camponoti-sp-nov-and-streptomyces-cuticulae-sp-nov-isolated-from-the-cuticle-of-camponotus-japonicus-mayr
#14
Chenyu Piao, Weiwei Zheng, Yao Li, Chongxi Liu, Liying Jin, Wei Song, Kai Yan, Xiangjing Wang, Wensheng Xiang
Two novel actinomycetes, designated strains 2C-SSA16(2)(T) and 1C-GS8(T), were isolated from the cuticle of Camponotus japonicus Mayr, collected from Northeast Agricultural University, Heilongjiang Province, north China. Both of them contained genes (involved in antibiotics biosynthesis) of the ketosynthase (KS) and methyl malonyl transferase domains (PKS-I) and the adenylation domain (NRPS). A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates 2C-SSA16(2)(T) and 1C-GS8(T) exhibited 98...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28378143/calr-is-required-for-the-expression-of-t6ss2-and-the-adhesion-of-vibrio-parahaemolyticus-to-hela-cells
#15
Lingyu Zhang, George Osei-Adjei, Ying Zhang, He Gao, Wenhui Yang, Dongsheng Zhou, Xinxiang Huang, Huiying Yang, Yiquan Zhang
Vibrio parahaemolyticus expresses one major virulence determinant T6SS2, which is constituted into three putative operons, i.e., VPA1027-1024, VPA1043-1028, and VPA1044-1046. CalR, a LysR-type transcriptional regulator, was originally identified as a repressor of the swarming motility and T3SS1 gene expression. As shown in this study, CalR binds to the promoter-proximal region of each of the three operons to activate their transcription, and moreover, CalR activates the adhesion of V. parahaemolyticus to HeLa cells...
April 4, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28378142/functional-characterization-and-transcriptional-analysis-of-icd2-gene-encoding-an-isocitrate-dehydrogenase-of-xanthomonas-campestris-pv-campestris
#16
Ying-Chuan Chiang, Chao-Tsai Liao, Shin-Chiao Du, Yi-Min Hsiao
Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In the genome of Xanthomonas campestris pv. campestris, the phytopathogen that causes black rot in cruciferous plants, two putative IDH genes, icd1 and icd2, have been annotated. Their physiological roles in X. campestris pv. campestris are unclear. In this study, the icd2 gene from X. campestris pv. campestris was characterized in detail. We demonstrated genetically that icd2 gene encodes a functional IDH, and is involved in virulence as well as bacterial attachment...
April 4, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28374062/insights-into-metabolism-and-sodium-chloride-adaptability-of-carbaryl-degrading-halotolerant-pseudomonas-sp-strain-c7
#17
Vikas D Trivedi, Anahita Bharadwaj, Madhushri S Varunjikar, Arminder K Singha, Priya Upadhyay, Kamini Gautam, Prashant S Phale
Pseudomonas sp. strain C7 isolated from sediment of Thane creek near Mumbai, India, showed the ability to grow on glucose and carbaryl in the presence of 7.5 and 3.5% of NaCl, respectively. It also showed good growth in the absence of NaCl indicating the strain to be halotolerant. Increasing salt concentration impacted the growth on carbaryl; however, the specific activity of various enzymes involved in the metabolism remained unaffected. Among various enzymes, 1-naphthol 2-hydroxylase was found to be sensitive to chloride as compared to carbaryl hydrolase and gentisate 1,2-dioxygenase...
April 3, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28364274/quorum-quenching-properties-of-actinobacteria-isolated-from-malaysian-tropical-soils
#18
Kavimalar Devaraj, Geok Yuan Annie Tan, Kok-Gan Chan
In this study, a total of 147 soil actinobacterial strains were screened for their ability to inhibit response of Chromobacterium violaceum CV026 to short chain N-acyl homoserine lactone (AHL) which is a quorum sensing molecule. Of these, three actinobacterial strains showed positive for violacein inhibition. We further tested these strains for the inhibition of Pseudomonas aeruginosa PAO1 quorum sensing-regulated phenotypes, namely, swarming and pyocyanin production. The three strains were found to inhibit at least one of the quorum sensing-regulated phenotypes of PAO1...
March 31, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28357475/iscr2-is-associated-with-the-dissemination-of-multiple-resistance-genes-among-vibrio-spp-and-pseudoalteromonas-spp-isolated-from-farmed-fish
#19
Yixiang Xu, Chao Wang, Gang Zhang, Jingjing Tian, Ying Liu, Xihui Shen, Jie Feng
58 multiresistant strains representing diverse genera were isolated from farmed fish in an aquaculture facility. Resistant rates of strains harboring ISCR2, an insertion sequence type element, were higher than those in which this element was absent. Full genome sequencing of a Vibrio isolate containing ISCR2 confirmed that it is associated with multiple resistance genes, many of which are of clinical relevance. We describe the structural variation within ISCR2, and its distribution throughout multiple diverse aquatic genera, including Vibrio, Shewenalla, Pseudoalteromonas and Psychrobacter, suggesting the potential role of ISCR2 in disseminating antibiotic resistance...
March 29, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28357474/new-advances-in-exopolysaccharides-production-of-streptococcus-thermophilus
#20
REVIEW
Yanhua Cui, Xu Jiang, Mengyuan Hao, Xiaojun Qu, Tong Hu
Streptococcus thermophilus is the most important thermophilic dairy starter, and is widely used in the dairy industry. Streptococcus thermophilus exopolysaccharides received wide attention over recent decades, because they can improve the properties of the dairy product and confer beneficial health effects. The understanding of the regulatory and biosynthetic mechanisms of EPS will improve the EPS biosynthesis, increase the productivity of EPSs, and develop EPSs with desirable properties. The structure of EPSs is the focus of this study...
March 29, 2017: Archives of Microbiology
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