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Archives of Microbiology

Vinaya Kizhakkepatt Kizhakkekalam, Kajal Chakraborty
The rich diversity of marine macroalgae and their associated bacterial flora represent a potential reservoir of bioactive compounds with valuable biotechnological and pharmaceutical use. Heterotrophic bacteria associated with the intertidal macroalgae were isolated and evaluated for their pharmacological properties using various in vitro models. Among 148 cultivable isolates, more than 50% were dominated by γ-Proteobacteria and Firmicutes, wherein 53 of them showed consistent antibacterial activity against a broad spectrum of clinically significant pathogens...
November 7, 2018: Archives of Microbiology
Xiaoguang Wang, Denis Tamiev, Jagannathan Alagurajan, Alan A DiSpirito, Gregory J Phillips, Mark S Hargrove
Nitrate and nitrite reduction are of paramount importance for nitrogen assimilation and anaerobic metabolism, and understanding the specific roles of each participating reductase is necessary to describe the biochemical balance that dictates cellular responses to their environments. The soluble, cytoplasmic siroheme NADH-nitrite reductase (Nir) in Escherichia coli is necessary for nitrate/nitrite assimilation but has also been reported to either "detoxify" nitrite, or to carry out fermentative ammonification in support of anaerobic catabolism...
November 7, 2018: Archives of Microbiology
Jaewoo Yoon, Mina Yasumoto-Hirose, Hiroaki Kasai
A novel marine flavobacterial species, designated 3KA7-17T , was isolated from the hard coral Acropora formosa D. collected in Japan. The strain was pale-orange pigmented, Gram-stain negative, strictly aerobic, coccus shaped, and non-motile. Preliminary analysis based on the 16S rRNA gene sequence revealed an affiliation with the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (96.0%) to Seonamhaeicola algicola Gy8T . The DNA G + C content was 34.3 mol%...
November 1, 2018: Archives of Microbiology
Chinmoy Ghosh, Avik Sarkar, Kumari Anuja, Manash C Das, Abhik Chakraborty, Junaid Jibran Jawed, Priya Gupta, Subrata Majumdar, Birendranath Banerjee, Surajit Bhattacharjee
Genomic instability resulting from oxidative stress responses may be traced to chromosomal aberration. Oxidative stress suggests an imbalance between the systemic manifestation of reactive free radicals and biological system's ability to repair resulting DNA damage and chromosomal aberration. Bacterial infection associated insult is considered as one of the major factors leading to such stress conditions. To study free radical responses by host cells, RAW 264.7 macrophages were infected with non-pathogenic M...
November 1, 2018: Archives of Microbiology
Shaohua Shi, Lei Tian, Shangqi Xu, Li Ji, Fahad Nasir, Xiujun Li, Zhiping Song, Chunjie Tian
The fungicides used to control diseases in cereal production can have adverse effects on non-target microbial communities, with possible consequences for plant health and productivity. Although we know that fungicides affect microbial community structure and soil activities, it is unclear how crop cultivars have altered the impact of fungicides on rhizomicrobiomes. In this study, the rhizosphere bacterial and fungal communities and structures of cultivated crops and their wild relatives were studied by Illumina MiSeq sequencing analysis...
October 25, 2018: Archives of Microbiology
Eldho Paul, Abhishek Albert, Sasikumar Ponnusamy, Sangeetha Venkatesan, Selvam Govindan Sadasivam
Lactobacillus plantarum WCFS1 (L. plantarum WCFS1) is commonly used as a potential cell factory because of its 'generally recognized as safe' status. The plasmid instability and the presence of antibiotic selection marker complicate the application of genetically modified L. plantarum in human clinical trials. In the present study, we aimed to integrate oxalate decarboxylase (oxdC) gene of Bacillus subtilis origin by targeted chromosomal mutation in L. plantarum using mobile genetic element Ll.LtrB as a therapeutic tool against calcium oxalate stone disease...
October 22, 2018: Archives of Microbiology
Juan Francisco Sánchez-López, Joaquín González-Ibarra, Juan Ignacio Macías-Segoviano, Mayra Cuéllar-Cruz, Aurelio Álvarez-Vargas, Carmen Cano-Canchola, Everardo López-Romero
Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphogenesis and activity of glucosamine-6-phosphate (GlcN-6-P) synthase. Under conditions of yeast development, 15 µM CR abolished conidia (CN) germination, but when yeast cells were first obtained in the absence of the dye and then post-incubated in its presence, yeasts rapidly differentiated into mycelial cells...
October 9, 2018: Archives of Microbiology
Bing Huang, Kun Cheng, Limin Zhang, Ying Huang, Changting Liu
A novel Marinactinospora strain JX35-4T was isolated from red soil which was collected from Wushan, northern Jiangxi Province, China. Analysis of the 16S rRNA gene sequences showed that strain JX35-4T belongs to the genus Marinactinospora and formed a distinct phylogenetic clade with Marinactinospora thermotolerans SCSIO 00652T and Marinactinospora endophytica YIM 690053T with sequence similarity of 96.97% and 96.42%, respectively. The strain was Gram-positive and formed branched substrate hyphae with no fragmentation, and abundant aerial hyphae that differentiated into long spore chains, and short rod-shaped spores...
October 8, 2018: Archives of Microbiology
Tian-Fu He, Lang-Hong Wang, De-Bao Niu, Qing-Hui Wen, Xin-An Zeng
In this study, the antimicrobial mechanism of cinnamaldehyde (CIN) against Gram-negative Escherichia coli ATCC 25922 (E. coli) based on membrane and gene regulation was investigated. Treatment with low concentration (0, 1/8, 1/4, 3/8 MIC) of CIN can effectively suppress the growth of E. coli by prolonging its lag phase and Raman spectroscopy showed obvious distinction of the E. coli after being treated with these concentration of CIN. The determination of relative conductivity indicated that CIN at relatively high concentration (0, 1, 2, 4 MIC) can increase the cell membrane permeability, causing the leakage of cellular content...
October 6, 2018: Archives of Microbiology
Yanlei Zhu
This study aimed to identify Ammodendron bifolium endophytic bacteria, and to evaluate promoting mechanism of selected isolates on seed germination and their effects on host osmotic-stress tolerance. Forty-five strains were isolated from A. bifolium and were classified into 13 different genera by 16S rDNA gene sequence analysis. AY3, AY9 and AG18, which were identified as Staphylococcus, Kocuria, Bacillus sp., promoted host seed ethylene release during germination. Ethrel and 1-aminocyclopropane-1-carboxylic acid (ACC) imitated the effect of AY3, AY9 and AG18 on seed germination...
October 4, 2018: Archives of Microbiology
Xiao-Tian Quan, Qing-Zhen Liu, Muhammad Zubair Siddiqi, Wan-Taek Im
A Gram-stain-negative, strictly aerobic, non-motile, ivory colored and rod-shaped bacterium (designated Gsoil 652T ) isolated from ginseng cultivating soil, was characterized using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 652T was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Phylogenetic analysis, based on 16S rRNA gene sequences similarities, indicated that Gsoil 652T belongs to the genus Caballeronia of the family Burkholderiaceae and was most closely related to Caballeronia choica LMG 22940T (98...
October 4, 2018: Archives of Microbiology
Tomás Santos, H Susana Marinho, Luisa Cyrne
Myo-inositol is a precursor of several membrane phospholipids and sphingolipids and plays a key role in gene regulation in Saccharomyces cerevisiae (S. cerevisiae). Here, we tested whether H2 O2 was affecting the levels of the inositol transporters and thus inositol uptake. In S. cerevisiae cells adapted to H2 O2 Itr1-GFPp accumulated in the plasma membrane until 20 min, concomitantly with an inhibition of its internalization. Exposure to H2 O2 did not alter Itr2-GFPp cellular levels and induced only an 8% decrease at 10 min in the plasma membrane...
October 3, 2018: Archives of Microbiology
Souhir Abdelkrim, Salwa Harzalli Jebara, Omar Saadani, Manel Chiboub, Ghassen Abid, Khediri Mannai, Moez Jebara
In this study, two populations of leguminous plants Lathyrus sativus were grown in four soils that were collected from sites differently contaminated by heavy metals. Evaluations included basic soil properties, concentrations of major nutrients and four metals (copper, zinc, lead and cadmium) in these soils. Investigation of Lathyrus sativus response to contamination showed that the increase of heavy metal concentration in soils affected biomass of plant, number of nodules and plant metal uptake. Heavy metal tolerance of 46 isolated bacteria from the root nodules was evaluated and demonstrated that the maximum concentration of Cd, Pb, Cu and Zn tolerated by strains were 0...
October 1, 2018: Archives of Microbiology
Jong-Pyo Kang, Yue Huo, Yeon-Ju Kim, Jong-Chan Ahn, Joon Hurh, Dong-Uk Yang, Deok-Chun Yang
A novel bacterial strain designated DCY116T was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. Strain DCY116T , belongs to the genus Rhizobium, and is closely related to Rhizobium yantingense H66T (98.3%), Neorhizobium huautlense S02T (98.2%), Rhizobium soli DS-42T (98.1%), Rhizobium smilacinae PTYR-5T (97.9%), and Neorhizobium alkalisoli CCBAU 01393T (97.9%) based on 16S rRNA gene sequence analysis. Analysis of the housekeeping genes atpD, recA, and glnII showed low levels of sequence similarity (96...
September 27, 2018: Archives of Microbiology
Mayara Luciana Sallas, Mônica Pezenatto Dos Santos, Wilson Aparecido Orcini, Érica Boarato David, Rita Luiza Peruquetti, Spencer Luiz Marques Payão, Lucas Trevizani Rasmussen
Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus granting the gene a functional or nonfunctional status; in other words, the functional status of the oipA gene seems to be associated with the development of gastric diseases. This study sought to detect the presence of the oipA gene and to determine its functional status in patients with gastric diseases...
September 26, 2018: Archives of Microbiology
Jaroslav Čepl, Vladimír Scholtz, Jiřina Scholtzová
Serratia marcescens forms different colony patterns under distinct conditions. One of them is the concentric fountain-shaped pattern with pigmented center followed by unpigmented ring and pigmented rim. In this work, we study this pattern formation by construction of the mathematical model able to display this pattern based on putative metabolical traits, supported by series of experiments and by references. A pattern formation of such colony type depends on the disposition of glucose and amino acids, and is accompanied by a pH change in the agar medium...
September 25, 2018: Archives of Microbiology
Dhiraj Kumar Chaudhary, Dockyu Kim, Jaisoo Kim
A novel yellow-colored, Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive, and rod-shaped psychrotolerant bacterium, designated strain PLR-18-3T , was isolated from Arctic soil and was subjected to polyphasic taxonomic study. Cells were able to grow at 0-30 °C, pH 6.0-10.5, and 0-3.0% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, this Arctic strain belonged to the genus Flavobacterium, with the closest neighbor being Flavobacterium noncentrifugens R-HLS-17T (96...
September 20, 2018: Archives of Microbiology
Simone Marcelletti, Marco Scortichini
A complementary taxonomic and population genetic study was performed to delineate genetically and ecologically distinct species within the Pseudomonas syringae complex by assessing 16 strains including pathovar strains that have converged to infect Prunus spp. trees, and two outgroups. Both average nucleotide identity and genome-to-genome distance comparison methods revealed the occurrence of distinct genomospecies, namely 1, 2, 3 and 8 (sensu Gardan et al.), with the latter two being closely related. Strains classified as P...
September 18, 2018: Archives of Microbiology
Yaqian Wu, Junying An, Ying Liu, Yaling Wang, Wenbin Ren, Zhijia Fang, Lijun Sun, Ravi Gooneratne
Bacteriocin CAMT2, produced by Bacillus amyloliquefaciens ZJHD3-06, has been shown to exhibit protective activity against important food spoilage and food-borne bacterial pathogens. This study was conducted to investigate the mode of action of bacteriocin CAMT2 against highly pathogenic Listeria monocytogenes ATCC 19111. The addition of bacteriocin CAMT2 at 64 AU/ml inhibited L. monocytogenes ATCC 19111. An efflux of K+ ions, lactic acid dehydrogenase and an increase in extracellular electrical conductivity was observed in CAMT2-treated L...
September 10, 2018: Archives of Microbiology
Christos T Chasapis
Tetrahymena is a unicellular microbial eukaryotic organism that has been used extensively in toxicology and environmental research. This work attempts to model for the first time the wiring of proteins involved in cellular mechanisms of Cd toxicity in Tetrahymena thermophila. 1975 high-confidence PPIs between 68 Cd-binding proteins and 422 partners were inferred through a novel structural systems biology approach that utilizes comparative analysis between Tetrahymena and other eukaryotes for which experimentally supported protein interactomes exist...
September 7, 2018: Archives of Microbiology
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