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Archives of Microbiology

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https://www.readbyqxmd.com/read/28799127/evaluation-of-the-antimicrobial-activity-of-a-blend-of-monoglycerides-against-escherichia-coli-and-enterococci-with-multiple-drug-resistance
#1
Immacolata Anacarso, Andrea Quartieri, Riccardo De Leo, Andrea Pulvirenti
Bacterial antibiotic resistance is a natural phenomenon, seriously affecting the treatment of infections. The biggest danger is that current antibiotics are not able to eradicate the resistant strains. In recent years, alternative antibacterial substances are being sought, which can help in these cases. Fatty acids and monoglycerides are known among the natural substances for their antimicrobial properties and, important detail, bacteria do not develop resistance to them. In this work, we studied the antimicrobial effects of a monoglyceride blend against some multi-resistant Enterococci and Escherichia coli strains...
August 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28799031/spirosoma-metallum-sp-nov-isolated-from-an-automobile-air-conditioning-system
#2
Dong-Uk Kim, Hyosun Lee, Suyeon Lee, Sooyeon Park, Jung-Hoon Yoon, So Yoon Park, Jong-Ok Ka
A Gram-stain-negative and yellow-pigmented bacterial strain, designated TX0653(T), was isolated from an automobile evaporator core collected in Korea. The cells were aerobic and rod-shaped. The strain grew at 10-28 °C (optimum, 25 °C), at pH 6.0-7.5 (optimum, 6.5), and in the presence of 0-1% (w/v) NaCl (optimum, 0%). Phylogenetically, the strain was related to members of the genus Spirosoma (95.1-90.8% 16S rRNA sequence similarity) and distantly related to Spirosoma pulveris JSH5-14(T) (95.1%), Spirosoma fluviale MSd3(T) (95...
August 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28795230/improved-multiplex-pcr-primers-for-rapid-identification-of-coagulase-negative-staphylococci
#3
Jonguk Kim, Jisoo Hong, Jeong-A Lim, Sunggi Heu, Eunjung Roh
Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified as S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. pasteuri, S. saprophyticus, S. warneri, and S. xylosus. Although 16S rRNA sequencing is universally accepted as a standard for bacterial identification, the method did not effectively discriminate closely related species, and additional DNA sequencing was required...
August 9, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28785779/bioprospecting-marine-actinomycetes-for-multidrug-resistant-pathogen-control-from-rameswaram-coastal-area-tamil-nadu-india
#4
Femina Wahaab, Kalidass Subramaniam
A potent Streptomyces bacillaris strain RAM25C4 was isolated for controlling methicillin-resistant Staphylococcus aureus and multidrug-resistant bacteria such as Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa. A total of 131 actinomycetes were isolated from the Rameswaram coastal region, Tamil Nadu, India. Among 131 actinomycetes, maximum number of actinomycetes (55%) isolated at the distance of 3-6 m from seashore. Out of 131 actinomycetes, 85% of the actinomycetes exhibited different degree of antagonistic activity against test pathogens...
August 7, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28780591/revealing-strategies-of-quorum-sensing-in-azospirillum-brasilense-strains-ab-v5-and-ab-v6
#5
Josiane Fukami, Julia Laura Fernandes Abrantes, Pablo Del Cerro, Marco Antonio Nogueira, Francisco Javier Ollero, Manuel Megías, Mariangela Hungria
Azospirillum brasilense is an important plant-growth promoting bacterium (PGPB) that requires several critical steps for root colonization, including biofilm and exopolysaccharide (EPS) synthesis and cell motility. In several bacteria these mechanisms are mediated by quorum sensing (QS) systems that regulate the expression of specific genes mediated by the autoinducers N-acyl-homoserine lactones (AHLs). We investigated QS mechanisms in strains Ab-V5 and Ab-V6 of A. brasilense, which are broadly used in commercial inoculants in Brazil...
August 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28780590/alpha-ketoglutarate-enhances-freeze-thaw-tolerance-and-prevents-carbohydrate-induced-cell-death-of-the-yeast-saccharomyces-cerevisiae
#6
Maria M Bayliak, Olha V Hrynkiv, Roksolana V Knyhynytska, Volodymyr I Lushchak
Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stresses in the yeast Saccharomyces cerevisiae. Growth on AKG-supplemented medium prevented a loss of viability and improved fermentative capacity of yeast cells after freeze-thaw treatment. The cells grown in the presence of AKG had higher levels of amino acids (e...
August 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28779470/halomarina-rubra-sp-nov-isolated-from-a-marine-solar-saltern
#7
Yao Zhou, Yang Li, Zhen-Zhen Lü, Heng-Lin Cui
Halophilic archaeal strain ZS-47-S(T) was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-S(T) was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03 M), and at pH 5.5-9.5 (optimum pH 6.5-7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was 5% (w/v). The major polar lipids were C20C20 and C20C25 diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether, and three unidentified glycolipids...
August 4, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28766036/evaluation-of-the-antimicrobial-activities-of-ultrasonicated-spinach-leaf-extracts-using-rapd-markers-and-electron-microscopy
#8
Ammar Altemimi, Naoufal Lakhssassi, Amer Abu-Ghazaleh, David A Lightfoot
Spinach (Spinacia oleracea L.) leaves represent an important dietary source of nutrients, antioxidants and antimicrobials. As such, spinach leaves play an important role in health and have been used in the treatment of human diseases since ancient times. Here, the aims were to optimize the extraction methods for recovering antimicrobial substances of spinach leaves, determine the minimum inhibitory concentrations (MICs) of the antimicrobial substances against Escherichia coli and Staphylococcus aureus and, finally, evaluate the effects of spinach leaves' antimicrobials on bacterial DNA using central composite face-centered methods...
August 1, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28762063/the-first-report-on-listeria-monocytogenes-producing-siderophores-and-responds-positively-to-n-acyl-homoserine-lactone-ahl-molecules-by-enhanced-biofilm-formation
#9
Milind Mohan Naik, Purva Bhangui, Chinmay Bhat
Listeria monocytogenes are Gram-positive well-known emerging food-borne pathogens causing listeriosis in humans. In the present study, we have isolated biofilm-forming Listeria sp. from utensils used by a local milk collection dairy society at Usgao Goa, which collects milk for Goa dairy. Through biochemical tests and 16S rRNA sequence analysis, the bacterium was confirmed to be L. monocytogenes and designated as strain BN3, having GenBank accession number MF095110. We report for the first time Gram-positive L...
July 31, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28748277/kribbella-sindirgiensis-sp-nov-isolated-from-soil
#10
Fadime Ozdemir-Kocak, Kamil Isik, Salih Saricaoglu, Hayrettin Saygin, Kadriye Inan-Bektas, Demet Cetin, Kiymet Guven, Nevzat Sahin
A Kribbella strain FSN23(T) was isolated from soil sample which was collected from Caygoren Dam lakeside located in Sındırgı, Turkey. The isolate was investigated using a polyphasic approach consisting of numeric, chemotaxonomic and molecular analysis. The isolate indicated chemotaxonomic, morphological and phylogenetic properties associated with members of the genus Kribbella. Phylogenetic analysis based on the 16S rRNA sequence of the strain demonstrated that the strain forms a subclade with K. aluminosa HKI 0478(T) and K...
July 26, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28741076/detection-of-verticillium-species-in-swedish-soils-using-real-time-pcr
#11
Georgios Tzelepis, Sarosh Bejai, Muhammad Naeem Sattar, Arne Schwelm, Jonas Ilbäck, Johan Fogelqvist, Christina Dixelius
Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V...
July 24, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28735377/study-of-the-correlation-between-fengycin-promoter-expression-and-its-production-by-bacillus-subtilis-under-different-culture-conditions-and-the-impact-on-surfactin-production
#12
Yazen Yaseen, Frédérique Gancel, Max Béchet, Djamel Drider, Philippe Jacques
This work aimed to rely expression of the fengycin promoter to fengycin production under different culture conditions. To this end, Bacillus subtilis BBG208, derived from BBG21, which is a fengycin overproducing strain carrying the green fluorescent protein (GFP) under the control of fengycin promoter, was used to assess the effects of different carbon and nitrogen sources on surfactin and fengycin production and the fengycin promoter expression. The data showed that some carbon sources oriented synthesis of one family of lipopeptides, while most of the nitrogen sources allowed high co-production of fengycin and surfactin...
July 22, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28733885/a-simple-and-rapid-in-vitro-test-for-large-scale-screening-of-fungal-endophytes-from-drought-adapted-australian-wild-plants-for-conferring-water-deprivation-tolerance-and-growth-promotion-in-nicotiana-benthamiana-seedlings
#13
Khondoker M G Dastogeer, Hua Li, Krishnapillai Sivasithamparam, Michael G K Jones, Stephen J Wylie
Some fungal endophytes confer novel phenotypes and enhance existing ones in plants, including tolerance to water deprivation stress. A range of fungal endophytes was isolated from wild Nicotiana plants growing in arid parts of northern Australia. These were screened for ability to enhance water deprivation stress tolerance by inoculating seedlings of the model plant N. benthamiana in two in vitro tests. Sixty-eight endophyte isolates were co-cultivated with N. benthamiana seedlings on either damp filter paper or on agar medium before being subjected to water deprivation...
July 21, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28707037/variability-of-composition-and-effects-of-essential-oils-from-rhanterium-adpressum-coss-durieu-against-mycotoxinogenic-fusarium-strains
#14
Fatiha Elhouiti, Djilali Tahri, Djalila Takhi, Mohamed Ouinten, Christian Barreau, Marie-Noëlle Verdal-Bonnin, Isabelle Bombarda, Mohamed Yousfi
The antifungal potency of the essential oils of Rhanterium adpressum was evaluated against four mycotoxigenic strains of the genus Fusarium. The essential oils were obtained, separately, by hydro-distillation of the aerial parts of R. adpressum (leaves and flowers). The parts were collected during the period of bloom (3 months) for 3 years. The GC-MS analysis revealed thirty-six compounds for the essential oils, divided into four classes of chemical compounds, with variable percentages according to the month of extraction...
July 13, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28702688/pseudomonas-aestus-sp-nov-a-plant-growth-promoting-bacterium-isolated-from-mangrove-sediments
#15
Rafael L F Vasconcellos, Suikinai Nobre Santos, Tiago Domingues Zucchi, Fábio Sérgio Paulino Silva, Danilo Tosta Souza, Itamar Soares Melo
Strain CMAA 1215(T), a Gram-reaction-negative, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 × 1.3-1.9 µm) bacterium, was isolated from mangrove sediments, Cananéia Island, Brazil. Analysis of the 16S rRNA gene sequences showed that strain CMAA 1215(T) forms a distinct phyletic line within the Pseudomonas putida subclade, being closely related to P. plecoglossicida ATCC 700383(T), P. monteilii NBRC 103158(T), and P. taiwanensis BCRC 17751(T) of sequence similarity of 98...
July 12, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28695265/conservation-of-erwinia-amylovora-pathogenicity-relevant-genes-among-erwinia-genomes
#16
Luigimaria Borruso, Marco Salomone-Stagni, Ivan Polsinelli, Armin Otto Schmitt, Stefano Benini
The Erwinia genus comprises species that are plant pathogens, non-pathogen, epiphytes, and opportunistic human pathogens. Within the genus, Erwinia amylovora ranks among the top 10 plant pathogenic bacteria. It causes the fire blight disease and is a global threat to commercial apple and pear production. We analyzed the presence/absence of the E. amylovora genes reported to be important for pathogenicity towards Rosaceae within various Erwinia strains genomes. This simple bottom-up approach, allowed us to correlate the analyzed genes to pathogenicity, host specificity, and make useful considerations to drive targeted studies...
July 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28688010/quorum-sensing-molecules-production-by-nosocomial-and-soil-isolates-acinetobacter-baumannii
#17
Demet Erdönmez, Abbas Yousefi Rad, Nilüfer Aksöz
Acinetobacter species remain alive in hospitals on various surfaces, both dry and moist, forming an important source of hospital infections. These bacteria are naturally resistant to many antibiotic classes. Although the role of the quorum sensing system in regulating the virulence factors of Acinetobacter species has not been fully elucidated, it has been reported that they play a role in bacterial biofilm formation. The biofilm formation helps them to survive under unfavorable growth conditions and antimicrobial treatments...
July 7, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28685236/engineered-cry1ac-cry9aa-hybrid-bacillus-thuringiensis-delta-endotoxin-with-improved-insecticidal-activity-against-helicoverpa-armigera
#18
Jigar V Shah, Rakeshkumar Yadav, Sanjay S Ingle
Recombinant Bt construct was prepared by exchange of pore forming domain I with cry1Ac to cry9Aa gene by overlap extension PCR (OE-PCR) technique. Construction of cry1Ac-cry9Aa was accomplished by six base pair homology at 3' ends of PCR products of domain I of cry1Ac and domain II and III of cry9Aa. The recombinant toxin was also modified by deletion of N-terminal alpha helix-1 of recombinant toxin. Both Cry toxins were expressed in E. coli BL21(DE3) plysS and purified by His-tag purification. Upon insect bioassay analysis against devastating crop pest Helicoverpa armigera, toxicity of recombinant toxin was found around fivefold higher than native Cry1Ac while alpha helix-1 deleted N-terminal modified toxin did not resulted in significant increase in toxicity...
July 6, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28669069/cyanobacterial-and-rhizobial-inoculation-modulates-the-plant-physiological-attributes-and-nodule-microbial-communities-of-chickpea
#19
Radha Prasanna, Balasubramanian Ramakrishnan, Kaur Simranjit, Kunal Ranjan, Amrita Kanchan, Firoz Hossain, Lata Nain
The present investigation aimed to understand the influence of two plant growth promoting cyanobacterial formulations (Anabaena-Mesorhizobium ciceri biofilm and Anabaena laxa), along with Mesorhizobium ciceri, on the symbiotic performance of five each of desi- and kabuli-chickpea cultivars. Inoculation with cyanobacterial formulations led to significant interactions with different cultivars, in terms of fresh weight and number of nodules, the concentration of nodular leghemoglobin, and the number of pods. The inoculant A...
July 1, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28653084/bacillus-alcaliphilum-sp-nov-a-bacterium-isolated-from-a-soda-lake
#20
Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe, Sasikala Chintalapati, Venkata Ramana Chintalapati
Two novel (14B(T) and 7B) Gram-stain-positive, rod-shaped, motile and endospore-forming bacterial strains were isolated from Lonar soda lake, India. Based on 16S rRNA gene sequence analysis, the strains 14B(T) and 7B were identified as belonging to the class Firmibacteria and were most closely related to Bacillus halodurans LMG 7121(T) (99.7 and 99.8%, respectively), Bacillus okuhidensis LMG 22468(T) (99.1 and 99.2%, respectively) and other members in the genus Bacillus (<97.0%). However, the DNA-DNA relatedness studies indicated that the strains 14B(T) and 7B were distantly related to B...
June 26, 2017: Archives of Microbiology
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