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Annals of Clinical and Laboratory Science

Kilmer S McCully
The formation of phosphoadenosine phosphosulfate (PAPS) is accomplished by the action of the enzyme 3'-phosphoadenosine 5'-phosphosulfate synthase (PAPSS) in two sequential reactions, consisting of (1) reaction of inorganic sulfate with adenosine triphosphate (ATP) to form adenosine phosphosulfate (APS) and pyrophosphate and (2) reaction of APS with inorganic phosphate to form PAPS and adenosine diphosphate (ADP). The hydrolysis of guanosine triphosphate (GTP) is coordinated with synthesis of APS in a reaction sequence which provides the chemical energy for synthesis of APS...
December 2016: Annals of Clinical and Laboratory Science
Nuri Lee, Ji-Eun Kim, Hyun Ju Yoo, JaYoon Gu, Hyori Kim, Junho Chung, Youngil Koh, Hyun Kyung Kim
We present a case of acquired dysfibrinogenemia caused by an autoantibody that inhibited fibrin polymerization in a patient previously diagnosed with MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis, stroke-like episodes). The patient showed prolonged PT, aPTT, and thrombin time. There was no factor deficiency but fibrinogen antigen and activity were decreased. ELISA for detection of fibrinogen antibodies were performed and IgG purified from the patient's plasma bound to fibrinogen more strongly than did control IgG, indicating the presence of a fibrinogen-specific antibody...
December 2016: Annals of Clinical and Laboratory Science
Junmei Hao, Cuiping Zhang, Qinghua Cao, Jiarui Zou, Chaofu Wang
We are reporting one case of multiple pulmonary adenofibromas in a 57-year-old non-smoking female. Ten well-circumscribed masses were identified in both lungs. The masses are characterized by gland-like structures lined by a single layer of simple cuboidal or columnar epithelium. The stroma is abundant and demonstrates compact spindle-cells. The epithelial component is generally positive for CK7, TTF-1, Napsin A. The stromal component displays expression of vimentin, desmin, SMA, h-CALD, ER, PR, Bcl-2, and is negative for CD34, CD117, CD99...
December 2016: Annals of Clinical and Laboratory Science
Mingxing Sui, Yanling Pei, Dong Li, Qiaori Li, Peining Zhu, Tianmin Xu, Manhua Cui
Cervical minimal deviation adenocarcinoma (MDA) is a rare variant of cervical adenocarcinoma that is difficult to diagnose due to the deep location, endogenous growth pattern, deceptively benign appearance of tumor cells, and lack of connection to human papillomavirus (HPV). Cytological evaluation and biopsies offer suboptimal detection and transvaginal sonography or Magnetic Resonance Imaging (MRI) only reveal multiple lesions that mimic multiple benign nabothian cysts. Besides, standard screening, diagnostic tools, and treatments are not established...
December 2016: Annals of Clinical and Laboratory Science
Jun Zou, Da-Nian Ji, Yan Shen, Jian-Long Guan, Song-Bai Zheng
AIMS: To examine the expression of miRNAs and mRNAs of heme oxygenase 1 (HO-1) in patients with active intestinal Behcet's disease (BD). METHODS: Peripheral blood mononuclear cells (PBMCs) were obtained from BD patients with active ileocecal ulcers or from healthy donors. Expression levels of four miRNAs were studied using real-time PCR. The levels of mRNAs of ho-1, bach1, and CD14 were measured by qRT-PCR. Serum levels of cytokines were analyzed by ELISA. RESULTS: Among four miRNAs, only levels of miR-196a2 were significantly decreased from BD patients with active ileocecal ulcers as compared with healthy controls...
December 2016: Annals of Clinical and Laboratory Science
Caihua Zu, Tao Liu, Guoliang Zhang
OBJECTIVE: To investigate the effects of microRNA-506 (miR-506) on malignancy of colorectal carcinoma (CRC) cells and to elucidate the underlying mechanism. METHODS: Human colorectal carcinoma cell lines SW480, SW620, HCT116, and HT29 were served as model. Five experimental groups are established in this study, including cell control, pcDNA3 blank vector control, miR-506 over-expression, pSIH1 blank vector control, and miR-506 suppression groups. Quantitative reverse transcription PCR (qRT-PCR) assay was performed to measure miR-506 level...
December 2016: Annals of Clinical and Laboratory Science
Eric R Rosenbaum, Soumya Pandey, Terry O Harville, Gina A Drobena, Michele Cottler-Fox
BACKGROUND: Panel reactive antibody (PRA) reduction protocols are used to decrease anti-HLA antibodies with concomitant PRA monitoring as a measure of successful treatment prior to organ and haploidentical blood and marrow transplant (BMT). We hypothesized that the more sensitive flow cytometry (FC) based assays for PRA [FlowPRA(®) and Luminex(®) based Single Antigen Bead (SAB)] would also correlate with the ability to find compatible platelets for allosensitized recipients. METHODS: A female patient with myelodysplastic syndrome and a high HLA class I PRA [>90% PRA and cPRA by complement-dependent cytotoxicity (CDC) assay and Flow PRA] required allogeneic BMT...
December 2016: Annals of Clinical and Laboratory Science
Yu Bai, Brian S Castillo, Amanda Tchakarov, Miguel A Escobar, Bryan A Cotton, John B Holcomb, Robert E Brown
People of the Jehovah's Witness faith believe that they shall "abstain from blood." Because of this belief, we encounter the challenges from Jehovah's Witness patients who actively seek medical care for themselves and their children, but refuse the transfusion of blood products, which may result in increased morbidity and mortality in this patient population. With the development/availability of new hemostatic/coagulation products and the advances in medical technology, we, in collaboration with our clinical colleagues and our local Jehovah's Witness leadership, have developed a clinical guideline comprising medical protocol and surgical strategy for patients refusing blood products...
December 2016: Annals of Clinical and Laboratory Science
Sanghoo Lee, Joo-Hoon Kim, Seol-A Kim, Yeoun-Soo Sun, Anna Lee, Seo-Jin Park, Yun-Tae Kim, Kyoung-Ryul Lee, Young-Jin Kim
BACKGROUND: Total 25-hydroxyvitamin D (25(OH)D) is well-known to be a reliable biomarker of human vitamin D status, with the recognition of widespread vitamin D insufficiency in general populations. The aims of this study are to validate a fast and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 25(OH)D2 and 25(OH)D3 in serum and to compare two automated immunoassays with the LC-MS/MS method. METHODS: Samples were prepared by protein precipitation with ethanol including 25(OH)D3-d6, followed by a liquid-liquid extraction with hexane...
December 2016: Annals of Clinical and Laboratory Science
Ana S Holley, John H Miller, Peter D Larsen, Scott A Harding
Low activity levels of the antioxidant enzyme, glutathione peroxidase (GPx), have been implicated in adverse clinical outcomes in coronary artery disease. A potential mechanistic link for this relationship is that low GPx activity predisposes patients to thrombotic complications due to impaired reactive oxygen species (ROS) metabolism. GPx potentially regulates the bioavailability of nitric oxide (NO) - a potent platelet inhibitor - therefore indirectly affecting platelet activation. This study examined the relationship between levels of GPx activity, ROS, and platelet activation in 51 acute coronary syndrome (ACS) patients...
December 2016: Annals of Clinical and Laboratory Science
Thomas B Martins, Michael E Bandhauer, Diane M Wilcock, Harry R Hill, Patricia R Slev
BACKGROUND: The clinical utility of serum IgG measurement in the diagnosis of allergy and food-induced hypersensitivity has been largely discredited. Recent studies, however, have shown that specific IgG can inhibit IgE mediated allergies, and may play a role in allergen specific desensitization. Accurate reference intervals for IgG specific allergens have not been widely established and are needed for better interpretation of serum antibody concentrations. In this study we established 64 IgG reference intervals for 48 common food allergens, 5 venoms, and 11 molds...
December 2016: Annals of Clinical and Laboratory Science
Mohammad F Shaikh, William F Morano, John Lee, Elizabeth Gleeson, Blake D Babcock, Josef Michl, Ehsan Sarafraz-Yazdi, Matthew R Pincus, Wilbur B Bowne
The mouse/murine protein, MDM2, and its human homolog, HDM2, are important negative regulators of the p53 tumor suppressor protein. In normal, untransformed cells, MDM2 levels are tightly regulated to control expression of p53 and apoptosis. Conversely, MDM2 expression appears inherently higher in multiple types of cancer cells, thereby supporting its role as a suppressor of p53 pro-apoptotic activity. MDM2 amplification ranges between two- and ten-fold as reported in brain, breast, lung, and soft tissue tumors...
December 2016: Annals of Clinical and Laboratory Science
Xudong Zhao, Jing Wu, Yan Zhang, Lin Zhu, Xiaojie Lu
Intestinal mucosal injury can be frequently observed in the patients suffering from subarachnoid hemorrhage (SAH). Labile zinc and inflammatory mediators participate in many pathophysiological processes. This study aimed to investigate the increase of intestinal labile zinc, wet/dry weight ratio, and the expression of inflammatory mediators after SAH. As a result, SAH caused a gradual increase of intestinal labile zinc as demonstrated by fluorescence staining with Zinpyr-4 (ZP4). The levels of TNF-α and IL-6 and the intestinal wet/dry weight ratios were higher in the SAH groups compared to the control group following SAH (p<0...
December 2016: Annals of Clinical and Laboratory Science
Sulaiman Ali Al Yousef, Khalid Abdullah Ali AbdelRahim, Asmaa O Ahmed, Khalid Salmeen Almaary, Ahmed Mohamed Ali Mohamed
BACKGROUND: Tuberculosis (TB) is an infectious disease that is caused by Mycobacterium tuberculosis (M.tb). TB has high morbidity and mortality around the world. OBJECTIVE: To evaluate molecular-based methods performed directly on stool samples for the diagnosis of pulmonary tuberculosis (PTB) and to determine the susceptibility to Rifampicin (RMP) and Isoniazid (INH). STUDY DESIGN: This is a descriptive study evaluating the performance of the PCR-based method for direct PTB diagnosis and to determine the susceptibility of RMP and INH using stool samples from PTB patients...
December 2016: Annals of Clinical and Laboratory Science
Deepshikha Nag Chowdhury, Youssef Botros, Vincent A DeBari, Walid Baddoura, Chandra B Chandran
Previous studies have shown a higher prevalence of malignancy in patients with diabetes mellitus (DM) and chronic kidney disease (CKD). The purpose of this study was to investigate the prevalence of adenomatous colon polyps (ACP) as they occur in subjects with DM and coexisting CKD. This is a retrospective cohort study of patients with DM (n=565) who had undergone colonoscopy between 2000-2010. The cohort was further bifurcated into those with CKD (n=296) and those with normal renal function (n=269). Presence or absence of ACP was measured in both groups...
December 2016: Annals of Clinical and Laboratory Science
Dilek Arpaci, Engin Karakece, Aysel Gurkan Tocoglu, Hasan Ergenc, Gonul Gurol, Ihsan Hakki Ciftci, Ali Tamer
PURPOSE: Although the relationship between atherosclerosis and overt hypothyroidism has been confirmed, it remains controversial in cases of subclinical hypothyroidism. Higher TSH and similar T4 suggest differences in set-points or differences due to diagnostic limitations regarding subclinical hypothyroidism. Endothelial dysfunction (ED) is a marker rather than a precursor of cardiovascular disease. Asymmetric dimethylarginine (ADMA) and endocan are known as novel markers of ED in various diseases...
December 2016: Annals of Clinical and Laboratory Science
Eman K Ebrahim, Magda M Assem, Ahmed I Amin, Mahmoud M Kamel, Yomna M El Meligui, Ayman M Metwally
OBJECTIVES: To detect FMS-like tyrosine kinase-3 internal tandem duplicate (FLT3 ITD) mutation, Myeloproliferative leukemia virus oncogene (cMPL) and Ephrin A 4 receptor (EphA4) expressions in Acute myeloid leukemia (AML) and their correlation to patient's clinicopathological characteristics and survival. METHODS: RNA was extracted from blood samples of 58 AML patients (39 adults and 19 children) and 20 age and sex matched controls. FLT3 ITD mutation, cMPL and EphA4 expression was studied using RT-PCR and correlated to the clinical and survival data of the patients...
December 2016: Annals of Clinical and Laboratory Science
Athanasia Alexoudi, Sophia Zachaki, Chrysa Stavropoulou, Stavroula Gavrili, Chara Spiliopoulou, Stavroula Papadodima, Clementine E Karageorgiou, Constantina Sambani
Oxidative stress plays a pivotal role in the pathogenesis of multiple sclerosis (MS). Inactivating polymorphism of genes encoding detoxification enzymes, such as NQO1 and GSTP1 could influence susceptibility to MS. The monoclonal antibody natalizumab is an effective treatment in MS. Natalizumab's efficacy in MS patients with regard to NQO1 and GSTP1 genetic polymorphisms is investigated. 130 patients with definite MS according to the Mc Donald's criteria treated monthly with natalizumab were included in the study...
December 2016: Annals of Clinical and Laboratory Science
Handong Wang, Hongbin Ni, Sufang Han, Wu Xu, Jing Wang, Baoyu Yuan, Tiansheng Zhu, Wei Jin, Weibang Liang, Jianwu Dai
Traumatic facial nerve injury, an important cause of facial paralysis, has a number of adverse effects, including facial muscle dysfunction and facial asymmetry. It has been demonstrated in our previous work that native human NT-3 fused with a collagen-binding domain (CBD-NT-3) could bind to collagen, specifically to exert neurotrophic effects, promoting axonal regeneration. To evaluate the effect of CBD-NT-3 in inducing facial nerve regeneration and functional recovery, the differing effects of CBD-NT-3 and native neurotrophin-3 (NAT-NT-3) were observed using the results of facial nerve functional recovery, electrophysiological testing, and axonal and myelin changes in a rat model of facial nerve crush injury...
December 2016: Annals of Clinical and Laboratory Science
Tomoko Maeda, Arisa Date, Mikio Watanabe, Yoh Hidaka, Yoshinori Iwatani, Toru Takano
In our previous studies, we established a method to analyze cells collected by fluorescence-activated cell sorting (FACS), named mRNA quantification after FACS (FACS-mQ), in which cells are labeled with fluorescent dyes in a manner that minimizes RNA degradation, and then cells sorted by FACS are examined by analyzing their gene expression profile. In this study, we examined methods to maximize the yield of recovered RNA after in-tube immunocytochemistry in addition to RNA analysis using a small dose of extracted RNA...
December 2016: Annals of Clinical and Laboratory Science
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