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Annals of Clinical and Laboratory Science

Zhe Fan, Yingyi Zhang, Jiyong Pan, Shuang Wang
BACKGROUND: Acute appendicitis (AA) is a common condition in general surgery, often with serious consequences such as septicemia and intra-abdominal infection. Mean platelet volume (MPV) - a platelet parameter - could be an index of inflammation. We carried out the present study to perform a meta-analysis of AA and MPV. METHODS: A systematic search was conducted in databases of PubMed and Embase from 1966 to 2015. Weighted mean difference and 95% confidence interval (CI) were then calculated...
November 2017: Annals of Clinical and Laboratory Science
Binara Assylbekova, Esther Elishaev, Rohit Bhargava
Unusual variants of endometrial endometrioid adenocarcinoma can be diagnostically challenging and pose difficulties to differentiate from their benign and malignant mimickers. Here we describe an entity that has been well documented in the literature, yet little is known about true outcome due to lack of studies with a large number of cases. Diffusely infiltrative or "adenoma-malignum-like" is an uncommon pattern of invasion in endometrioid endometrial adenocarcinoma which could be confused with adenomyosis...
November 2017: Annals of Clinical and Laboratory Science
Muhammad Riaz Khan, Ihtisham Bukhari, Tang Junxiang, Liu Hui, Niaz Muhammad, Cui Fan, Jiangsheng Zhu, Mian Wu
Sex chromosomal mosaicism has been considered as a major cause of human sexual differentiation disorders, like partial virilization and ambiguous genitalia. 45,X/46,XX, 45,X/46,XY and 46,XY/47,XXY are three most common sex chromosome mosaics associated with human ambiguous genitalia. Here, we report the case of a 3-year-old boy with ambiguous genitalia, bilateral cryptorchidism, and with an inguinal hernia. G banded cytological karyotyping and FISH analyses revealed that the patient has extremely rare and novel sex chromosome mosaic 45,X/45,Y/46,XY/46,YY/47,XYY karyotype...
November 2017: Annals of Clinical and Laboratory Science
Fernando Acosta, Yumi Kojima, Mary Yang
A 67-year-old woman presented with post-menopausal bleeding. Physical examination and imaging revealed a cervical mass with extensive pelvic involvement and supraclavicular lymphadenopathy. Clinical diagnosis was stage IVB cervical carcinoma. Cervical biopsy revealed high-grade squamous intraepithelial lesion and epithelioid tumor cells in the stroma. Lymph node biopsy revealed metastasis by similar epithelioid tumor cells. The epithelioid tumor cells were positive for p16, CD68, and CD163 on immunohistochemical study...
November 2017: Annals of Clinical and Laboratory Science
Rong Xiang, Ran Du, Shuai Guo, Jie-Yuan Jin, Liang-Liang Fan, Ju-Yu Tang, Zheng-Bing Zhou
Split hand/foot malformation (SHFM) is a congenital heterogeneous disorder with prominent limb deficiency. Seven loci have been identified to associate with SHFM, including SHFM1 to SHFM6 and SHFM/SHFLD. SHFM3 is an autosomal dominant disease, of which the pathogenesis is closely related to the genomic rearrangements at 10q24.We described two Chinese patients with the SHFM3 phenotype by high-resolution SNP array technology. We detected a 534kb microduplication at 10q24 encompassing TLX1, LBX1, BTRC and POLL, and a 600kb duplication with TLX1, LBX1, BTRC, POLL, and FBXW4 located...
November 2017: Annals of Clinical and Laboratory Science
Ji Yoon Han, Ja Hyun Jang, In Goo Lee, Soyoung Shin, Joonhong Park
Mutations in SCN8A, which codes for the voltage-gated sodium channel NaV1.6, have been described in relation to infantile onset epilepsy with developmental delay and cognitive impairment. Here, we report the case of an infant and her father with early onset benign familial infantile epilepsy, but without cognitive or neurological impairment. In this patient, diagnostic exome sequencing (DES) identified a heterozygous mutation (c.4427G>A; p.Gly1476Asp) in the SCN8A gene. This mutation, confirmed by Sanger sequencing, effects a highly conserved amino acid...
November 2017: Annals of Clinical and Laboratory Science
Ginell R Post, Elizabeth Guillory, Curtis L Wade, Susan E LeSourd, Steven R Post
BACKGROUND: We previously showed that the prothrombin time (PT), but not activated partial thromboplastin time (APTT), is correlated with serum immunoglobulin level in patients with plasma cell neoplasms. METHODS: To determine if the observed effect of serum immunoglobulins on PT was reagent and/or method dependent, PT and APTT were measured in plasma samples obtained from patients referred to the Myeloma Institute using mechanical (STAR Evolution; Diagnostica Stago) and optical clot detection (ACL TOP 500 analyzer; Instrumentation Laboratory) and manufacturer provided reagents...
November 2017: Annals of Clinical and Laboratory Science
Yaolin Zhou, Alicia R Armstead, Gina M Coshatt, Nita A Limdi, Shuko Harada
BACKGROUND: CYP2C19 polymorphisms contribute about 12% of the variability in the antiplatelet effect of clopidogrel, which is commonly prescribed for patients undergoing percutaneous coronary intervention. For these patients, rapid turnaround time of CYP2C19 genotyping may be critical. We validated and compared the performance of two point-of-care CYP2C19 genotype tests, Nanosphere Verigene CYP2C19 Nucleic Acid Test and Spartan RX CYP2C19 System. MATERIALS AND METHODS: Our CLIA certified Molecular Diagnostic Laboratory performed 99 Verigene tests and 108 Spartan RX CYP2C19 assays...
November 2017: Annals of Clinical and Laboratory Science
Zexin Li, Xitao Li, Shaoda Lin, Yongsong Chen, Shuhua Ma, Yucai Fu, Chiju Wei, Wencan Xu
OBJECTIVES: We found that activation of the nicotinic acid receptor GPR109A, expressed by the MIN6 murine pancreatic β cell line, inhibits nitric oxide accumulation induced by IFN-γ and TNF-α, implicating an anti-inflammatory effect of GPR109A in MIN6 cells. Nevertheless, the mechanism of its anti-inflammatory effect is still unknown. In this study, we used palmitic acid to stimulate MIN6 cells to induce inflammatory cytokine production and explored the mechanism by which GPR109A exerts anti-inflammatory effects...
November 2017: Annals of Clinical and Laboratory Science
Yugang Jiang, Kai Huang, Xixia Lin, Qinchang Chen, Shaoyan Lin, Xiaomin Feng, Chaohui Zhen, Min Huang, Shenming Wang
OBJECTIVES: The purpose of this study is to evaluate whether Berberine can suppress the inflammatory response in atherosclerosis lesions and its potential associated signaling pathways and mechanism of action. METHODS: We isolated human peripheral blood mononuclear cells. After co-culturing them with ox-LDL stimulated cells, ROS was measured by its fluorescence intensity and NADPH oxidase activity was detected by the OD value from the spectrophotometer. Human peripheral blood mononuclear cells were then pretreated with different concentrations of berberine after treatment with NLRP3 activator ATP...
November 2017: Annals of Clinical and Laboratory Science
Răzvan A Ciocan, Cristina Drugan, Claudia D Gherman, Cristina-Sorina Cătană, Andra Ciocan, Tudor C Drugan, Sorana D Bolboacă
BACKGROUND: Chitotriosidase is an enzyme secreted by activated macrophages. This study aims to investigate the usefulness of circulating chitotriosidase activity as a marker of inflammatory status in patients with critical limb ischemia (CLI). MATERIALS AND METHODS: An observational gender-matched case-control study was conducted on patients hospitalized with the primary diagnosis of CLI, as well as a control group. The control group consisted of healthy volunteers...
November 2017: Annals of Clinical and Laboratory Science
Shujun Guo, Helong Wang, Yulan Luo, Jing He, Hua Ma, Lin Shen, Jie Tang, Qiang Fang, Hongtao Wang, Zhongqing Qian, Chuanwang Song
miR-20b is a member of the miR-106a-363 gene cluster, located on the X chromosome. miR-20b regulates the expression of multiple genes in vivo and it is closely related to the occurrence and development of many diseases. These diseases include inflammatory diseases and tumor development, amongst others. However, few studies have focused on the regulation of the miR-20b gene itself. In this study, we are using the miRBase database to obtain the upstream 2000 bp sequence of the miR-20b precursor. Bio-informatics software P-MATCH 1...
November 2017: Annals of Clinical and Laboratory Science
Yan Hu, Ruifang Wu, Huan Li, Yiling Gu, Weixia Wei
PURPOSE: The purpose of this study is to investigate the roles of matrix metalloproteinases (MMPs) and collagen in female patients with pelvic organ prolapse (POP). METHODS: Sixty POP patients comprised the POP group (23 cases of grade II prolapse (POP-subgroup 1) and 37 cases of grade III-IV prolapse (POP-subgroup 2)]; another 35 non-POP patients comprised the control group. Reverse transcription polymerase chain reaction and immunohistochemistry were used to detect the relative mRNA and protein expression of MMP-1, MMP-8, tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), and type I and III collagen in the anterior vaginal wall tissues...
November 2017: Annals of Clinical and Laboratory Science
Shuangfeng Zhang, Liang Ma, Yafeng Chen, Huan Xu, Suning Chen, Wenzhong Wu
BACKGROUND: The hematopoietically expressed homeobox (HHEX) is widely expressed in hematopoietic stem cells and is an essential transcription factor in embryonic development; however its role in hematopoiesis and leukemogenesis is poorly understood. We are thus exploring the association of HHEX and acute myeloid leukemia (AML). METHODS: The study included 56 AML patients and 12 normal bone marrows (NBMs). Real-time quantitative polymerase chain reaction (Q-PCR) was used to assess HHEX expression...
November 2017: Annals of Clinical and Laboratory Science
Mingxiu Guan, Yingna Tong, Xiaobin Liu, Dong Dong, Yunli Zhou
BACKGROUND: M2 type Pyruvate kinase (PKM2) is the key rate-limiting enzyme of glycolysis, and it mainly exists as a dimer in tumor cells. This study aims to establish an enzyme kinetic assay for serum tumor M2 pyruvate kinase (TuM2-PK), and to evaluate its diagnostic value in breast cancer. METHODS: The catalytic kinetics of Pyruvate Kinase (PK) was examined. Its allosteric regulation property and Michaelis constant were then measured. Next, the levels of TuM2-PK in serum were detected and compared with results from an enzyme-linked immunosorbent assay (ELISA)...
November 2017: Annals of Clinical and Laboratory Science
Shiqing Cheng, Ying Ju, Fuyan Han, Yong Wang, Yanqiu Xu, Teng Qu, Zhiming Lu
The T-cell immunoglobulin and mucin domain containing molecule 3 (TIM-3) gene is an important immune regulatory molecule. In fact, studies have shown that polymorphisms in the (TIM-3) gene may be associated with various cancers. The goal of this study was to investigate whether the -1516G/T, -574G/T, or +4259T/G single-nucleotide polymorphisms (SNPs) in the TIM-3 gene contribute to a genetic susceptibility to invasive breast cancer in the Han ethnicity of northern China. Genotyping of the TIM-3 -1516G/T, -574G/T and +4259T/G were performed in 301 patients with invasive breast cancer and in 151 healthy individuals via a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay...
November 2017: Annals of Clinical and Laboratory Science
Xiaohong I Wang, Fengying Huang, Songlin Zhang
Loop electrosurgical excision procedure (LEEP) and cold knife cone (CKC) are often used for the treatment of high-grade cervical intraepithelial lesions. LEEP is an in-office procedure with less discomfort and fewer complications than CKC. However, concerns related to LEEP include the interpretability of the resection margins, positive margins, and the tissue fragmentation. Negative for dysplasia in LEEP or CKC specimens are not uncommon, which may be associated with lesion regression or interpretation errors...
November 2017: Annals of Clinical and Laboratory Science
Guo-Dong Zhu, Shi OuYang, Feng Liu, Zhi-Gang Zhu, Fu-Neng Jiang, Bei Zhang
AIMS AND BACKGROUND: Discs large homolog 1 (DLG1) belongs to the modular proteins Membrane Associated Guanylate Kinases (MAGUKs) and plays a major role in the formation of cell-cell junctions and cell polarity. DLG1 varies in expression and localization among malignancies. However, the clinical significance of DLG1 in the context of human colorectal cancer (CRC) remains unclear. The purpose of this study study is to determine the association of DLG1 with clinicopathological characteristics and prognosis of CRC patients...
November 2017: Annals of Clinical and Laboratory Science
Eta S Berner
The recent interest in diagnostic error in medicine was preceded almost fifty years ago by a focus on the problem-solving strategies of the experts of that time. This focus also extended to include the development of computer-based decision support systems to aid clinicians in diagnosis. Interest in medical errors was heightened with the publication of the Institute of Medicine report To Err is Human in 2000; however, there was still a relative neglect of the problem that diagnostic error presents. This article discusses the extent and types of diagnostic error in medicine, the challenges of studying diagnostic error, and strategies to address these errors...
November 2017: Annals of Clinical and Laboratory Science
Mahesheema Ali, Deepthi Rajapakshe, Liyun Cao, Sridevi Devaraj
Recent studies have reported that biotin interferes with certain immunoassays. In this study, we evaluated the analytical interference of biotin on immunoassays that use streptavidin-biotin in our pediatric hospital. We tested the effect of different concentrations of biotin (1.5-200 ng/ml) on TSH, Prolactin, Ferritin, CK-MB, β-hCG, Troponin I, LH, FSH, Cortisol, Anti-HAV antibody (IgG and IgM), assays on Ortho Clinical Diagnostic Vitros 5600 Analyzer. Biotin (up to 200 ng/mL) did not significantly affect Troponin I and HAV assays...
September 2017: Annals of Clinical and Laboratory Science
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