Olexander M Semenenko, Victoria V Lipson, Alina O Sadchenko, Olga V Vashchenko, Natalia A Kasian, Liliia V Sviechnikova, Longin M Lisetski, Mykola L Babak, Volodimir M Vakula, Olexander V Borisov, Yuliia V Holota, Sergiy O Zozulya, Petro O Borisko, Olexander V Mazepa
An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids with a (tert-butoxycarbonylamino)-3,6-dioxa-8-octanamine (Boc-DOOA) linkage has been developed. Reaction of N-(2-(2-(2-aminoethoxy)ethoxy)ethyl)-betulonamide with methotrexate resulted in a mixture of isomeric conjugates which were separated by column chromatography. Their structures and composition have been fully established by 1 H NMR, 13 C spectra, FAB mass spectrometry and elemental analysis. The identity of conjugates was confirmed by LC-MS data...
November 6, 2023: Steroids