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Ningbo Gong, Ying Wang, Baoxi Zhang, Dezhi Yang, Guanhua Du, Yang Lu
Solvatomorphism occurred in diosgenin solvates. A series of solvates including DMSO, DMF, DMAC, Methanol of diosgenin were obtained and crystallized in order to compare their structures and properties in the solid state. Single-crystal X-ray diffraction was used to analyze the crystal structures and the solvent types and the stoichiometric ratio of the diosgenin solvates. Hirshfeld surface analyses, especially dnorm surface and fingerprint plots, were used to determine intermolecular interactions in the crystal structure...
December 1, 2018: Steroids
Andy Pranata, Christopher C Fitzgerald, Olha Khymenets, Erin Westley, Natasha J Anderson, Paul Ma, Oscar J Pozo, Malcolm D McLeod
Doubly or bisconjugated steroid metabolites have long been known as minor components of the steroid profile that have traditionally been studied by laborious and indirect fractionation, hydrolysis and gas chromatography-mass spectrometry (GC-MS) analysis. Recently, the synthesis and characterisation of steroid bis(sulfate) (aka disulfate or bis-sulfate) reference materials enabled the liquid chromatography-tandem mass spectrometry (LC-MS/MS) study of this metabolite class and the development of a constant ion loss (CIL) scan method for the direct and untargeted detection of steroid bis(sulfate) metabolites...
December 1, 2018: Steroids
Quan Zhang, Lin-Fu Liang, Ze-Hong Miao, Bin Wu, Yue-Wei Guo
Four new polyhydroxylated steroids lobophysterols A-D (1-4), together with two known related compounds sarcophytosterol (5) and klyflaccisteroid B (6), were isolated from the South Chinese Sea soft coral Lobophytum sp. Their structures were elucidated on the basis of spectroscopic analysis and by comparison with those reported in the literature. In bioassay, compound 4 showed weak cytotoxicities against HT-29, SNU-398, and Capan-1 cells.
December 1, 2018: Steroids
Pilar Martín Escudero, Jesús A Muñoz-Guerra, Soledad Vargas García-Tenorio, Ester Serrano Garde, Ana B Solvevilla Navarro, Mercedes Galindo Canales, Nayade Prado, Manuel E Fuentes Ferrer, Cristina Fernández Pérez
This article studies the genetic influence of polymorphism of the UGT2B17 gen on the urinary steroid profile and its implications for the anti-doping field. The study presents the results of a triple-blind randomized placebo-controlled crossover trial with healthy athletes submitted to a single dose of 250mg of testosterone cypionate. Forty urine samples were collected from each participant. Mass spectrometry-based techniques commonly used in Anti-Doping laboratories, were employed to measure the urinary concentration and the Δδ13 C values of a selection of target compounds for testosterone (T) administration together with LH...
November 29, 2018: Steroids
Dagmara Klopotowska, Janusz Matuszyk, Joanna Wietrzyk
MiR-125b belongs to the class of microRNAs, which are short endogenous non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. Recently, it was reported that miR-125b was found to promote migration and invasion of MCF-7 cells and was involved in chemotherapeutic resistance. Decreasing miR-125b expression would have potential therapeutic significance in preventing dissemination of breast cancer cells. The objective of this study was to evaluate miR-125b expression levels in MCF-7 cells following treatment with 1,25-dihydroxyvitamin D3 (calcitriol) and 1,24-dihydroxyvitamin D3 (tacalcitol), active metabolite and synthetic analog of vitamin D3 , respectively...
November 29, 2018: Steroids
Usman M Ashraf, Edwin R Sanchez, Sivarajan Kumarasamy
Chicken Ovalbumin Upstream Promoter Transcription Factor II (COUP-TFII) is an orphan member of the nuclear receptor family of transcriptional regulators. Although hormonal activation of COUP-TFII has not yet been identified, rodent genetic models have uncovered vital and diverse roles for COUP-TFII in biological processes. These include control of cardiac function and angiogenesis, reproduction, neuronal development, cell fate and organogenesis. Recently, an emerging body of evidence has demonstrated COUP-TFII involvement in various metabolic systems such as adipogenesis, lipid metabolism, hepatic gluconeogenesis, insulin secretion, and regulation of blood pressure...
November 24, 2018: Steroids
Vladimir I Kalinin, Alexandra S Silchenko, Sergey A Avilov, Valentin A Stonik
Triterpene glycosides are known as characteristic metabolites of sea cucumbers (class Holothuroidea, phylum Echinodermata). The most of them have lanostane aglycones containing 18(20)-lactone, i.e. belongs to so-called holostane series. However non-holostane glycosides having no lactone (with the both normal and shortened side chains) or containing 18(16)-lactone function were also found in these invertebrates last a few decades. In addition, some very rare findings of the glycosides containing rearranged lanostane skeleton systems were reported...
November 22, 2018: Steroids
Yoko Nishimura, Kimihiko Moriya, Sumitaka Kobayashi, Atsuko Araki, Fumihiro Sata, Takahiko Mitsui, Sachiko Itoh, Chihiro Miyashita, Kazutoshi Cho, Masafumi Kon, Michiko Nakamura, Takeya Kitta, Sachiyo Murai, Reiko Kishi, Nobuo Shinohara
The ratio of the lengths of the 2nd and 4th digits (2D:4D) is considered an index of prenatal exposure to androgen. Indeed, androgen receptors have been linked to digit length, but estrogen receptors are rarely investigated in this context. Thus, we investigated the association between estrogen receptor 1 (ESR1) genetic polymorphisms and 2D:4D in school-aged children. The 2D:4D ratios were determined using Vernier calipers from photocopies of palms provided by 1,800 children aged 7 years who were enrolled in an ongoing prospective cohort study in Hokkaido, Japan...
November 20, 2018: Steroids
Sebastián N Jäger, Exequiel O J Porta, Guillermo R Labadie
A simple and efficient method to synthesize the immunogenic glycolipid BbGL1 is introduced. Two simple steps were required to obtain the desired product in good yield. First, a highly efficient glycosylation of cholesterol using galactosyl trichloroacetimidate as a donor was performed to produce cholesteryl-β-D-galactoside. Finally, an efficient palmitoylation on the C6-OH of the galactose of the synthesized saponin using sym-collidine and acyl chloride under microwave heating that produced BbGL1 in good yield...
November 20, 2018: Steroids
Bakhtiyar S Temirgaziyev, Karolína Kučáková, Yerassyl A Baizhigit, Michal Jurášek, Petr Džubák, Marián Hajdúch, Bohumil Dolenský, Pavel B Drašar, Borash I Tuleuov, Sergazy M Adekenov
20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.) Otth; sometimes Silene wolgensis (Willd.) Bess. ex Spreng. The complexation of the phytoecdysteroid with β-cyclodextrin was studied by NMR spectroscopy. By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2...
November 17, 2018: Steroids
Ilona Jurek, Ilona Góral, Krzysztof Gęsiński, Kamil Wojciechowski
The study discusses the effect of a quinoa seed coat extract on a cholesterol-based Langmuir monolayer mimicking the intercellular lipid mixture in the skin's outermost layer - stratum corneum. Besides cholesterol (CHOL), the monolayer contains also stearic acid (SA) and ceramide VI (CER), in a molar ratio of 10:14:14. Three quinoa extracts were tested for their surface activity: a) from the whole seed, b) from the dehulled seed, and c) from the seed coat. The latter shows significantly higher ability to reduce surface tension (increase surface pressure) than the others...
November 17, 2018: Steroids
Fernando P Filgueira, Núbia S Lobato, Denise L Nascimento, Graziela S Ceravolo, Fernanda R C Giachini, Victor V Lima, Ana Paula Dantas, Zuleica B Fortes, R Clinton Webb, Rita C Tostes, Maria Helena C Carvalho
Conjugated equine estrogens (CEE) have been widely used by women who seek to relieve symptoms of menopause. Despite studies results showing the protective effects against risk factors for cardiovascular diseases by naturally occurring estrogens, little is known about the vascular effects of equilin, one of the main components of CEE and not physiologically present in women. In this regard, the present study aims to compare the vascular effects of equilin in an experimental model of hypertension with those induced by the naturally occurring estrogens, 17β-estradiol...
November 17, 2018: Steroids
Muhammad Ayaz, Abdul Sadiq, Abdul Wadood, Muhammad Junaid, Farhat Ullah, Nadir Zaman Khan
Based on our previous studies on cytotoxic potentials of Polygonum hydropiper L, two steroidal compounds beta-sitosterol and stigmasterol were isolated from the most active fraction and were subjected to cell lines cytotoxicity. Isolated compounds were tested against HeLa, MCF-7 and NIH/3T3 cell lines following MTT assay. Furthermore, the compounds were also docked against tyrosine kinase enzyme to predict the binding mode of phytosterols in the active sites of the enzyme. Beta-sitosterol exhibited considerable cytotoxicity against NIH/3T3, HeLa and MCF-7 cell with 67...
November 13, 2018: Steroids
Justin X Nicoll, Andrew C Fry, Eric M Mosier
PURPOSE: To determine if there is differential expression and phosphorylation of the androgen receptor (AR), glucocorticoid receptor (GR), and mitogen-activated protein kinases (MAPK) in skeletal muscle at rest between males and females. METHODS: Ten college-aged males (mean ± SD; age = 22 ± 2.4 yrs, ht = 175 ± 7 cm, body mass = 84.1 ± 11.8 kg) and ten females (mean ± SD; age = 20 ± 0.9 yrs; ht = 169 ± 7 cm; body mass = 67...
November 7, 2018: Steroids
Taitusi Taufa, Peter T Northcote, Robert A Keyzers
NMR-directed investigation of the two sponge association between Stelletta crater and Desmacella dendyi has resulted in the isolation of two new members of the rare 4-methylidene class of sterols. Craterol A (1) and B (2) represent the first examples of natural products reported from the species S. crater. The isolation of these compounds challenges the role of 4-methylidene sterols as chemotaxonomic markers for the sponge genus Theonella.
November 7, 2018: Steroids
Ryuta Baba, Kenji Oki, Kazuhiro Kobuke, Kiyotaka Itcho, Hirofumi Okubo, Haruya Ohno, Masayasu Yoneda, Noboru Hattori
OBJECTIVE: Elevated midnight cortisol levels induced by non-suppressed ACTH levels may lead to false-positive results for hypercortisolism in patients with adrenal incidentaloma. We investigated whether plasma ACTH-associated high midnight serum cortisol levels are correlated with other endocrinological findings with respect to hypothalamic-pituitaryadrenal function or hypercortisolism status. METHODS: Two-hundred-forty-six patients with adrenocortical adenoma were evaluated via measurements of midnight ACTH and cortisol levels, a 1-mg dexamethasone suppression test (DST), and a cosyntropin-releasing hormone (CRH) stimulation test...
November 7, 2018: Steroids
Juan Manuel Agraz-Cibrian, Diana M Giraldo, Silvio Urcuqui-Inchima
Neutrophils are components of the innate immune system that participate in controlling infectious diseases through microbicidal mechanisms such as phagocytosis, degranulation and the release of neutrophil extracellular traps (NETs). NETs are DNA structures that are released through the decondensation and spreading of chromatin and the adherence of various proteins, including neutrophil elastase (NE), myeloperoxidase (MPO) and peptidyl arginine deiminase 4 (PDA4). Since NETs recovered after treatment of activated polymorphonuclear neutrophils can enhance IL-1β and IFN-α production by LPS-activated macrophages, they are thought to be keys to the host's defenses and inflammation...
November 7, 2018: Steroids
Ahmed M Ramadan, Ahmed Abdel Azeiz, Saeed Baabad, Sameh Hassanein, Nour O Gadalla, Sabah Hassan, Mardi Algandaby, Salwa Bakr, Thana Khan, Heba H Abouseadaa, Hani Mohammed Ali, Areej Al-Ghamdi, Gamal Osman, Sherif Edris, Hala Eissa, Ahmed Bahieldin
Most scientific studies on Calotropis procera refer to the plant as an important source of pharmaceutical compounds and its valuable benefits in medicine. One of the most important substances in this plant is the potential immunostimulant β-sitosterol (BS) that acts in improving human health. This study focused on the effects of lighting before and after irrigation on the BS accumulation pathway namely steroid biosynthesis. Studying the enzymes in BS biosynthetic pathway indicated the upregulation at dawn and predusk of the SMT2 and SMO2 genes encoding sterol methyltransferase 2 and methylsterol monooxygenase, two key enzymes in BS accumulation in C...
November 7, 2018: Steroids
Xiao-Long He, Li-Ting Wang, Xiang-Zhong Gu, Jie-Xin Xiao, Wen-Wei Qiu
A novel synthetic route of producing ursodeoxycholic acid (UDCA) and obeticholic acid (OCA) was developed through multiple reactions from cheap and readily-available cholic acid. The reaction conditions of the key elimination reaction of mesylate ester group were also investigated and optimized, including solvent, base and reaction temperature. In the straightforward synthetic route for preparation of UDCA and OCA, most of the reaction steps have high conversions with average yields of 94% and 92%, and overall yield up to 65% (7 steps) and 36% (11 steps) from cholic acid, respectively...
October 31, 2018: Steroids
Laurie S Conklin, Peter A Merkel, Lauren M Pachman, Hemang Parikh, Shefa Tawalbeh, Jesse M Damsker, David D Cuthbertson, Gabrielle A Morgan, Paul A Monach, Yetrib Hathout, Kanneboyina Nagaraju, John van den Anker, Carol A McAlear, Eric P Hoffman
Glucocorticoids are standard of care for many chronic inflammatory conditions, including juvenile dermatomyositis (JDM) and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). We sought to define pharmacodynamic biomarkers of therapeutic efficacy and safety concerns of glucocorticoid treatment for these two disorders. Previous proteomic profiling of patients with Duchenne muscular dystrophy (DMD) and inflammatory bowel disease (IBD) treated with glucocorticoids identified candidate biomarkers for efficacy and safety concerns of glucocorticoids...
October 21, 2018: Steroids
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