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Scandinavian Journal of Clinical and Laboratory Investigation

Niclas Rollborn, Torbjörn Åkerfeldt, Gunnar Nordin, Xiao Yan Xu, Aleksandra Mandic-Havelka, Lars-Olof Hansson, Anders Larsson
Hemoglobin A1c (HbA1c) is a frequently requested laboratory test and there is thus a need for high throughput instruments for this assay. We evaluated a new automated multicapillary zone electrophoresis instrument (Capillarys 3 Tera, Sebia, Lisses, France) for analysis of HbA1c in venous samples. Routine requested HbA1c samples were analyzed immunologically on a Roche c6000 instrument (n = 142) and then with the Capillarys 3 Tera instrument. The Capillarys 3 Tera instrument performed approximately 70 HbA1c tests/hour...
October 21, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Thore Hillig, Torben Kjær Nielsen, Steen Ingemann Hansen, Ann-Britt Nygaard, György Sölétormos
It remains unclear whether total prostate specific antigen (tPSA) or complex PSA (cPSA) has the best diagnostic performance. Additionally, the utility of percentage free PSA (%fPSA) is still debated. Our objectives were to compare the diagnostic performances of tPSA, cPSA, and %fPSA among patients referred from GP to an Urological Specialist and to investigate prognostic factors and survival in the cohort. A total of 1261 consecutive male patients without previously known prostate cancer (PCa) were referred to the same Department of Urology during June 2005 to August 2006...
October 20, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Jing Yu, Zhao-Juan Wang, Long-Hua Chen, Wen-Zhu Dong
The aim of this study was to ascertain the diagnostic value of serum squamous cell carcinoma antigen (SCCA) and SCCA-IgM for hepatocellular carcinoma (HCC). After a comprehensive search of PubMed and Web of Science databases, we identified eligible studies on the diagnostic value serum SCCAs for HCC. The quality of the eligible studies was assessed using the revised Quality Assessment for Studies of Diagnostic Accuracy (QUADAS-2) tool. The overall diagnostic value of SCCAs for HCC was pooled using a bivariate model...
October 20, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Pavel Šiarnik, Zuzana Čarnická, Zuzana Krivošíková, Katarína Klobučníková, Ingrid Žitňanová, Branislav Kollár, Marek Sýkora, Peter Turčáni
Hypercholesterolemia represents a risk factor for the development of atherosclerosis. Lipoprotein research has recently been focused on the phenomenon of atherogenic and non-atherogenic lipoproteins. The aim of this study was to explore the association of lipoprotein subfractions with a measure for endothelial function (represented by reactive hyperemia index [RHI]) and arterial stiffness (represented by augmentation index [AI]) in patients with acute ischemic stroke. We enrolled 51 patients with acute ischemic stroke...
October 19, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Árpád Lábadi, Ágnes Nagy, Árpád Szomor, Attila Miseta, Gábor L Kovács
No abstract text is available yet for this article.
October 14, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Tae-Dong Jeong, Eun-Jung Cho, Dae-Hyun Ko, Woochang Lee, Sail Chun, Ki-Sook Hong, Won-Ki Min
BACKGROUND: The aim of this study was to report the experience of large-scale performance evaluation of 238 Accu-Chek Inform II point-of-care (POC) glucose meters in a single medical setting. METHODS: The repeatability of 238 POC devices, the within-site imprecision of 12 devices, and the linearity of 49 devices were evaluated using glucose control solutions. The glucose results of 24 POC devices and central laboratory were compared using patient samples. RESULTS: Mean concentration of control solutions was 2...
October 14, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Per Lehnert, Pär I Johansson, Sisse R Ostrowski, Christian H Møller, Lia E Bang, Peter Skov Olsen, Jørn Carlsen
Whole blood coagulation and markers of endothelial damage were studied in patients with acute pulmonary embolism (PE), and evaluated in relation to PE severity. Twenty-five patients were enrolled prospectively each having viscoelastical analysis of whole blood done using thrombelastography (TEG) and Multiplate aggregometry. Fourteen of these patients were investigated for endothelial damage by ELISA measurements of Syndecan-1 (endothelial glycocalyx degradation), soluble endothelial Selectin (endothelial cell activation), soluble Thrombomodulin (endothelial cell injury) and Histone Complexed DNA fragments (endothelial cytotoxic histones)...
October 11, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Aleksander J Owczarek, Magdalena Olszanecka-Glinianowicz, Piotr Kocełak, Maria Bożentowicz-Wikarek, Aniceta Brzozowska, Małgorzata Mossakowska, Monika Puzianowska-Kuźnicka, Tomasz Grodzicki, Andrzej Więcek, Jerzy Chudek
BACKGROUND: The available literature suggests that circulating visfatin/Nicotinamide Phosphoribosyltransferase (NAMPT) level variability in humans is related to obesity, insulin resistance, inflammation, and lipid profile. The aim of the study was to assess the relationship between circulating visfatin/NAMPT, obesity, insulin resistance, inflammation, and lipid profile in a large population-based, elderly cohort, applying structural equation modeling. MATERIALS AND METHODS: The analysis included 2983 elderly participants of the PolSenior study with assessed total blood count, fasting concentrations of lipids, glucose, insulin, hs-CRP, interleukin-6, and visfatin/NAMPT (by ELISA), and calculated HOMA-IR...
October 7, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Øyvind Skadberg, Sverre Sandberg, Thomas Røraas, Per Hyltoft Petersen, Hilde Sellevoll, Einar Svarstad, Kristin Sæle, Kristin Moberg Aakre
BACKGROUND: Cardiovascular mortality is high in end stage renal disease (ESRD). This study aimed to: (1) calculate within-week within- and between-subject biological variation (CVI and CVG) for hs-cTn in ESRD; (2) determine the magnitude of hs-cTn concentration changes during haemodialysis (HD) treatment; and (3) compare the CVI and CVG to the within and between-subject variation of cTn concentration changes during HD treatments (CVDIFF-I and CVDIFF-G). METHODS: Serum samples were collected from 20 patients before and after 10 consecutive HD treatments...
October 5, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Michael Glas, Janine Viola Bauer, Hermann Eichler, Thomas Volk
Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10(-9 )L and a calcium concentration of 5 mmol L(-1)...
October 5, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Jonas E Sabroe, Mark B Ellebæk, Niels Qvist
Microdialysis is used for in vivo sampling of extracellular molecules. The technique provides a continuous and dynamic view of concentrations of both endogenous released and exogenous administered substances. Microdialysis carries a low risk of complications and has proven to be a safe procedure in humans. The technique has been applied in several clinical areas, including gastrointestinal surgery. Microdialysis may be used for studies of tissue metabolism, and the technique is also a promising tool for pharmacological studies of drug penetration into abdominal organ tissue and the peritoneal cavity...
October 5, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Mette Christensen, Rikke Fogt Madsen, Line Rosengreen Møller, Cindy Soendersoe Knudsen, Mie Hessellund Samson
INTRODUCTION: The aim of this study was to investigate and compare the stability of adrenocorticotrophic hormone (ACTH) in whole blood stored on ice and at room temperature for up to 48 hours. This study differs from previous studies by a larger data material. MATERIALS AND METHODS: EDTA-blood samples from 30 patients were collected, aliquoted and stored on ice or at room temperature for 0, 2, 4, 24, or 48 h before centrifugation, and the plasma was stored frozen until analysis...
October 5, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Omar Abuyaman, Niels Torring, Rima Obeid, Ebba Nexo
BACKGROUND: Human placenta expresses CD320, a receptor that ensures the uptake of holo-transcobalamin (holoTC). Soluble CD320 (sCD320) is present in the circulation and its concentration increases during pregnancy. AIMS: To investigate a possible association of sCD320, holoTC and total transcobalamin (TC) with the risk of subsequent preeclampsia using serum samples from asymptomatic first trimester pregnant women. Moreover, we aimed to establish reference intervals of the aforementioned biomarkers for first trimester pregnant women who remained healthy throughout pregnancy...
October 4, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Mats Eriksson, Gunnar Strandberg, Miklós Lipcsey, Anders Larsson
BACKGROUND: Intraosseous (IO) access can be established faster than a venous or arterial access when there is an urgent need for rapid initiation of treatment. The access can also be used to draw marrow samples. The aim of the present study was to evaluate the potential use of IO samples for enzyme determinations using a porcine model. MATERIALS AND METHODS: Bilateral tibial intraosseous cannulae and an arterial catheter were used for blood sampling from five healthy anesthetized pigs...
September 29, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Olesja Rissling, Steffen Bauer, Maria Shipkova, Petra Glander, Marco Mai, Pia Hambach, Klemens Budde
OBJECTIVES: Mycophenolic acid (MPA) is an immunosuppressive agent which is commonly used in a fixed dose regime in solid organ transplantation. For clinical trials and therapeutic drug monitoring measuring plasma concentrations is necessary. Also, stability issues have to be addressed. METHODS: We describe an isocratic, RP-based HPLC-UV method for simultaneous determination of MPA and its major metabolite Mycophenolic acid 7-o Glucuronide (MPAG) in human plasma...
September 27, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Adina Elena Stanciu, Anca Elena Hurduc, Marcel Marian Stanciu
OBJECTIVE: We aimed to investigate the effects of thyroid hormone withdrawal on N-terminal prohormone forms of atrial natriuretic peptide (NT-proANP) and brain natriuretic peptide (NT-proBNP) during radioiodine therapy in female patients with differentiated thyroid cancer (DTC). METHODS: Serum concentrations of thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), NT-proANP and NT-proBNP were measured in 51 female patients with DTC (48.7 ± 4.2 years) at three time-points: day of radioiodine therapy (t1 - under acute hypothyroidism), 5 days after radioiodine (t2 - under acute hypothyroidism) and 3 months after radioiodine (t3 - under TSH suppression)...
September 26, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Vanja Cejvanovic, Camilla Asferg, Laura Kofoed Kjær, Ulrik B Andersen, Allan Linneberg, Jan Frystyk, Trine Henriksen, Allan Flyvbjerg, Michael Christiansen, Allan Weimann, Jørgen Jeppesen, Henrik Enghusen Poulsen
OBJECTIVES: The aim of our study was to investigate if the 24-hour excretion of the urinary markers for oxidative stress to DNA and RNA, measured as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo), respectively, were increased in obese individuals with or without hypertension compared to lean controls. METHODS: A total of 63 obese hypertensive men (obeseHT), 40 obese normotensive men (obeseNT) and 27 lean normotensive men (leanNT) were included in the study...
September 26, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Line Brøndum, Brita Singers Sørensen, Jesper Grau Eriksen, Lise Saksø Mortensen, Simon Lønbro, Jens Overgaard, Jan Alsner
OBJECTIVE: To assess the usability of archived plasma and serum by multiplex (Luminex) analysis of circulating proteins (analytes) by evaluating the day to day variation, the effect of several freeze-thaw cycles, and the influence of the media and choice of anticoagulant. METHODS: Nineteen analytes in plasma and serum from 86 head and neck cancer patients and 33 controls were evaluated: EGFR, leptin, OPN, VEGFR-1, VEGFR-2, IL-2, IL-13, PDGF-bb, TNF, PAI-1, SDF-1a, IL-4, IL-6, IL-8, eotaxin, G-CSF, VEGF, GRO-a, and HGF...
September 26, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Ulrik Lausten-Thomsen, Michael Christiansen, Paula Louise Hedley, Cilius Esmann Fonvig, Theresa Stjernholm, Oluf Pedersen, Torben Hansen, Jens-Christian Holm
BACKGROUND: Adipokines are biologically active, low-molecular weight peptides, which play a major role in metabolic homeostasis in humans. Leptin has gained increasing attention in pediatrics as a biomarker for various metabolic pathologies. Yet, its usefulness is hampered by the relative lack of reference values from pediatric settings. Accordingly, this study aims to evaluate serum concentrations of leptin, soluble leptin receptor (sOB-R), and free leptin index (FLI) in healthy Danish schoolchildren aged 6-18 years and subsequently to establish reference intervals across sex and age groups...
November 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Murat Usta, Hale Aral, Ahmet Mete Çilingirtürk, Alev Kural, Ibrahim Topaç, Tuna Semerci, Mehmet Hicri Köseoğlu
Average of normals (AON) is a quality control procedure that is sensitive only to systematic errors that can occur in an analytical process in which patient test results are used. The aim of this study was to develop an alternative model in order to apply the AON quality control procedure to datasets that include qualitative values below limit of detection (LoD). The reported patient test results for tumor markers, such as CA 15-3, CA 125, and CA 19-9, analyzed by two instruments, were retrieved from the information system over a period of 5 months, using the calibrator and control materials with the same lot numbers...
November 2016: Scandinavian Journal of Clinical and Laboratory Investigation
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