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Scandinavian Journal of Clinical and Laboratory Investigation

Andreas Hillarp, Karin Strandberg, Fariba Baghaei, Inger Fagerberg Blixter, Kerstin M Gustafsson, Tomas L Lindahl
Edoxaban is an oral direct factor Xa inhibitor for prophylaxis and treatment of thromboembolic disorders. The effects on common coagulation assays are clinically valuable information and in certain clinical situations a quick assessment of the anticoagulant is wanted. Our aim was to investigate the effect of edoxaban on routine coagulation methods and evaluate anti-Xa assays, commonly used for other direct factor Xa inhibitors, for estimation of the drug concentration. Edoxaban was spiked to plasma samples from healthy subjects in the concentration range 0-742 µg/L and analyzed using different reagents for activated partial thromboplastin time (APTT) and prothrombin time (PT)...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Marevic Sanja, Petrik Jozsef, Popovic Grle Sanja, Cepelak Ivana, Grgic Ivana, Gorenec Lana, Stjepanovic Gordana, Laskaj Renata, Zidovec Lepej Snjezana
Cytokines are biological response modifiers involved in the pathophysiology of chronic obstructive pulmonary disease (COPD). This study investigated the potential use of cytokines as disease severity biomarkers in COPD patients and the possible effect of statin therapy on cytokine expression. Possible associations between cytokines, body mass index (BMI) and smoking have also been studied. Cytokines IFN-γ, IL-2, IL-12 p70, TNF-α, TNF-β, IL-4, IL-5, IL-6, IL-10, IL-1β and IL-8 were measured in the plasma of 100 clinically stable COPD patients using a fluorescent bead immunoassay on a flow cytometer...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Aline Louise Lemes, Josi Karla Amadeu, Cristina Salazar Berrocal, Juliana Lucena Schussel, Meltem Bakkal, José Miguel Amenábar
Oxidative stress biomarkers of oral and systemic diseases can be found in saliva. However, there is no uniformity for the saliva collection time in these kinds of analyses and saliva composition may change because of mechanical stimulation. Therefore, the aim of this work was to determine the effect of mechanical stimulation for 10 min on the concentrations of vitamin C, vitamin E, total antioxidants and total oxidants in saliva. Saliva samples from individuals of both sexes, aged between 18 and 38 years, were collected for 10 min at 2 minintervals...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Ilya Valerevich Smirnov, Irina Vladimirovna Gryazeva, Margarita Yurevna Vasileva, Irina Yurevna Krutetskaia, Olga Alexandrovna Shashkova, Marina Platonovna Samoylovich, Anastasia Yurevna Stolbovaya, Nellya Grigorevna Solodovnikova, Irina Evegnevna Zazerskaya, Dmitriy Igorevich Sokolov, Sergey Alexeevich Selkov, Vladimir Borisovich Klimovich
Soluble endoglin (sEng) is a fragment of a membrane-associated receptor (CD105) expressed on endothelial cells, mesenchymal stem cells and trophoblast cells. It is considered as a regulatory factor involved in the development of preeclampsia (PE) and cancer-associated neo-angiogenesis. The purpose of this study was to describe a new sandwich ELISA for sEng quantification. In contrast to three commercial kits, the new ELISA was able to quantify sEng not only in blood plasma and cell culture supernatants, but also in urine and cerebrospinal fluid...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Magnus H Jonsson, Ami Hommel, Aleksandra Turkiewicz, Ulf Ekelund, Olle Melander, Martin Englund, Peter Bentzer
Hip fractures in elderly carry a high mortality. Our objective was to test the hypothesis that plasma lactate concentration at hospital admission can be used to identify patients with a high risk for poor outcome. Hip fracture patients admitted to a university hospital in Sweden from January 2011 to August 2014 in whom a venous lactate was obtained at admission were included in this prospective observational study. Primary outcome measure was 30-d mortality and secondary outcome measure was a composite outcome of 30-d mortality and postoperative complications...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Dae-Hyun Ko, Eun-Jung Cho, Woochang Lee, Sail Chun, Won-Ki Min
Therapeutic drug monitoring of tacrolimus and cyclosporine is crucial to the success of organ transplantation. We evaluated the analytical performances and accuracy of two commercially available tacrolimus and cyclosporine assays (Roche ISD and Siemens) in comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 342 leftover whole blood samples requested for tacrolimus or cyclosporine assays were stored at -20 °C until analysis. Repeatability and between-run imprecision were evaluated using quality control materials provided by the manufacturer...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
August Bagge, Ulf Schött, Thomas Kander
The effect of omega-3 fatty acids on platelet aggregation and coagulation is highly unclear. Studies both support and refute the impacts of omega-3 fatty acids on prolonged bleeding time and platelet inhibition as well as its purported positive effects on cardiovascular disease. In a previous pilot study we suggested an inhibition of platelet aggregation measured with multiple electrode aggregometry. Following on that, the aim of the present study was to investigate the effects of supplementary high doses of omega-3 fatty acids on platelet aggregation and coagulation in a sample-size calculated number of healthy volunteers using Sonoclot, multiple electrode aggregometry, and flow-based Cellix instruments after 10 days of omega-3 fatty acid intake...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Eun Hye Cho, Rihwa Choi, Eun-Suk Kang, Hyung-Doo Park
IgG consists of four subclasses: IgG1, IgG2, IgG3, and IgG4. Changes in the serum concentration of each subclass reflect different clinical situations, and quantification of each subclass is important to assess patients' clinical states. Herein, we evaluated the analytical performance of the SPAPLUS turbidimetric analyzer (The Binding Site, Birmingham, UK) for IgG subclass. Precision, linearity, comparison with the BNII system (Siemens Healthineers, Erlangen, Germany), and reference interval were assessed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Sofie Sommer Hedegaard, Anna Christina Klein, Anne-Mette Hvas
The role of thrombophilia in paediatric thromboembolic disease remains controversial. The objective of the present study was to investigate the prevalence of thrombophilia and other risk factors among children with a thromboembolic event. We systematically examined the medical records and laboratory results of 126 children (≤15 years) referred to Aarhus University Hospital, Denmark with a recent thromboembolic event during 2005-2016. Among the 126 children with thrombosis, 99 (79%) had arterial thrombosis, 66 (52%) being in the neonatal period...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Erland Jørn Erlandsen, Else Randers
The aim of this study was to document the differences between two Roche creatinine measurement methods, the CREP2 test on the cobas c702 and the CREA PLUS test on the Modular P. Samples with creatinine isotope dilution mass spectrometry (IDMS) values were analyzed on both instruments. Method comparison using the remaining plasma samples was performed twice, using two different lot numbers of reagents and two different lot numbers of calibrators on both instruments. Medians and percentiles of the plasma creatinine values produced on the Modular P and cobas c702 from 2012 to 2017 were compared...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Bodil Bjørndal, Inge Bruheim, Vegard Lysne, Marie S Ramsvik, Per M Ueland, Jan E Nordrehaug, Ottar K Nygård, Rolf K Berge
Plasma concentrations of metabolites along the choline oxidation and tryptophan degradation pathways have been linked to lifestyle diseases and dietary habits. This study aimed to investigate how krill oil, a source of ω-3 polyunsaturated fatty acids (PUFAs) with a high phosphatidylcholine content, affected these parameters. The pilot study was conducted as a 28 days intervention in 17 healthy volunteers (18-36 years), who received a supplement of 4.5 g krill oil per day, providing 833 mg ω-3 PUFAs, and 1750 mg phosphatidylcholine...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Songlin Yu, Yicong Yin, Qian Cheng, Jianhua Han, Xinqi Cheng, Ye Guo, Dandan Sun, Shaowei Xie, Ling Qiu
Rapid and simple method for serum and urine iodine is necessary for iodine-related diseases. This study aimed to establish and validate a simple and rapid method for quantification of iodine in human urine and serum using inductively coupled plasma mass spectrometry (ICP-MS). Generally, samples were diluted using water with 1.5% isopropanol and 7 mmol hydrous ammonium before analysis by the ICP-MS. Performance of the method was validated, and the urine and serum iodine levels of 72 pregnant women were analyzed...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Zenthuja Sivalingam, Nils Erik Magnusson, Erik Lerkevang Grove, Anne-Mette Hvas, Steen Dalby Kristensen, Sanne Bøjet Larsen
Inflammation is an important mediator in the pathogenesis of atherosclerosis. Neutrophil gelatinase-associated lipocalin (NGAL) is a small glycoprotein secreted by neutrophils. NGAL regulates the activity of matrix metalloproteinase-9, which plays a role in plaque instability. It has therefore been hypothesised that NGAL may modulate inflammation and promote the development and progression of atherosclerosis. Our aim was to assess the predictive value of plasma NGAL in a prospective cohort study of 876 high-risk patients with stable coronary artery disease (CAD)...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Pia Afzelius, Stig Molsted, Lise Tarnow
To assess distal blood pressures (BPs) in ankle and toe, walking capacity, intermittent claudication symptoms, physical activity and self-rated health after intermittent vacuum treatment (IVT) for peripheral artery disease (PAD). Forty-eight patients with PAD were randomised to either a control (C)-group with lifestyle changes or lifestyle changes in combination with VacuMed® treatment thrice weekly for 6 weeks. Ankle and toe BPs were measured before, after 6 weeks of intervention and after 6 weeks follow-up...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Joakim Lindström, Anders E Henriksson
Regular measurement of prothrombin time (PT) as an international normalized ratio (INR) is of great importance in ensuring a safe Warfarin treatment due to the narrow therapeutic range. Hence, accurate results of the PT/INR analysis are crucial. Due to the high sampling load that patients on Warfarin experience, some prefer capillary sampling (from the tip of the finger) as an alternative to standard venous sampling (from the cubital fossa). The present study evaluated the method of capillary sampling compared to the standard venous method for plasma PT/INR measurement...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Line Dam Heftdal, Anne Gitte Loft, Oliver Hendricks, Alice Ashouri Christiansen, Berit Schiøttz-Christensen, Bodil Arnbak, Anne Grethe Jurik, René Østgård, Bent Winding Deleuran, Holger Jon Møller, Stinne Ravn Greisen
The chronic joint inflammation in axial spondyloarthritis (axSpA) is characterized by infiltration of activated macrophages. The haptoglobin-hemoglobin receptor CD163 and the mannose receptor CD206 are strongly expressed on M2c and M2a macrophages, respectively. We measured the soluble forms of the receptors (sCD163 and sCD206) in plasma (PL) in two axSpA cohorts. All patients fulfil the 2009 Assessment of SpondyloArthritis International Society (ASAS) classification criteria for axSpA and/or the 1984 modified New York criteria for ankylosing spondylitis...
September 4, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Karoline Winckler, Niels Wiinberg, Andreas Kryger Jensen, Birger Thorsteinsson, Louise Lundby-Christensen, Berit Lilienthal Heitmann, Gorm Boje Jensen, Lise Tarnow
Carotid intima-media thickness (IMT) and ankle brachial index (ABI) are non-invasive indicators of generalised atherosclerosis. The aim was to determine the association between carotid IMT and ABI in subjects with and without diabetes mellitus (DM), and to analyse specific age change-points. We included 2744 subjects from the Copenhagen City Heart Study (mean age (SD) 56.6 (17.2) years, 56.8% women and body mass index (BMI) 25.4 (4.1) kg/m2 ). Carotid IMT and ABI measurements were performed during the fifth examination...
August 3, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Misuk Ji, Mina Hur, Hee-Won Moon, Mikyoung Park, Yeo-Min Yun, Sang-Heon Lee
Anti-cyclic citrullinated peptide antibodies (anti-CCPs) are important diagnostic markers for rheumatoid arthritis (RA). We evaluated the analytical and clinical performance of the QUANTA Flash CCP3 (INOVA Diagnostics, USA), a fully automated third-generation anti-CCP assay, in comparison with three second-generation anti-CCP (CCP2) assays. A total of 300 sera (67 from RA patients, 64 from other rheumatic diseases, 43 from osteoarthritis [OA], and 126 from other conditions) were tested with QUANTA Flash CCP3, Kallestad Anti-CCP II (Bio-Rad, USA), Elecsys Anti-CCP (Roche Diagnostics GmbH, Germany), and ARCHITECT Anti-CCP (Abbott Diagnostics, USA)...
August 3, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Erdim Sertoglu, Ahmet Omma, Cigdem Yucel, Seda Colak, Sevinc Can Sandıkcı, Taner Ozgurtas
Behçet's disease (BD) is a rare, chronic, inflammatory disorder characterized by multisystemic vasculitis including mucocutaneous, neurologic, and ophthalmic involvement. Our aim is to compare vascular endothelial growth factor (VEGF) and soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) levels in BD, among the patients having or not having organ involvement, disease activation and especially vascular involvement. Fifty-five patients with BD, 25 of which were accompanied by vascular involvement, and 31 control subjects were included in the study...
July 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Haneen Khashoggi, Stefano Pignalosa, Carmelo Russo, Massimo Pieri, Sergio Bernardini
Glycated hemoglobin (HbA1c ) measurement provides the most important medium to long-term marker of time-averaged glycemic status. Its relationship to clinical outcome in diabetes has been convincingly demonstrated for both type 1 and type 2 diabetes. The main HbA1c measurement methods for clinical routine are as follows: ion-exchange chromatography; affinity chromatography, capillary electrophoresis, immunoassay and enzymatic methods. In this study, we evaluated the analytical performances of a new HPLC instrument (Tosoh HLC-723 G11 in the VAR mode) in HbA1c analysis and compared it with a capillary electrophoresis instrument (Sebia Capillarys 2 Flex Piercing)...
July 11, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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