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Scandinavian Journal of Clinical and Laboratory Investigation

Søren Kristiansen, Lennart Friis-Hansen, Claus Antonio Juel Jensen, Steen Ingemann Hansen
We verified the lactate dehydrogenase (LDH) reference interval (RI) provided by the Nordic Reference Interval Project (NORIP). The serum LDH concentration was analysed on the Dimension Vista 1500 system with an IFCC method with a bias of +2.1 % and +2.7 % against NFKK Reference Serum X and ERM-AD453/IFCC, respectively, showing verification of transference of the NORIP RI. Selective data mining in clinical laboratory information systems for retrospective serum LDH test results was used to calculate an indirect RI...
June 13, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Eva Rabing Brix Petersen, Mads Nybo
Tourniquets are widely used to make the vein more visible prior to blood collection. Venepuncture tourniquets are however a non-sterile and potentially reusable equipment. Several studies have shown that they are colonised by a variety of pathogenic bacteria and consecutively use of the same tourniquet on multiple patients will increase the risk of a nosocomial infection. This matter is however only scarcely studied. The objective of this study was to investigate the nationwide use of disposable and non-disposable venepuncture tourniquets and the standardised procedures for cleaning the tourniquets...
June 10, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Ji-Fang Guo, Yu Zhang, Qing-Xia Zheng, Yao Zhang, Hui-Hua Zhou, Lun-Meng Cui
Bioinformatics indicate that miR-223 regulates many genes associated with cholesterol metabolism, and it could also control high-density lipoprotein-cholesterol (HDL-C) uptake. As reported in previous study, miR-223 was found to be upregulated from human subjects with familial hypercholesterolaemia, however, it remains to be determined using a larger group of coronary heart disease (CHD) patients. Moreover, whether it correlates with severity of atherogenesis, has never been elucidated before. We aim to further explore the association between circulating miR-223 content and severity of CHD...
June 10, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Kirsten Sørensen, Jens Kjeldsen-Kragh, Henrik Husby, Çiğdem Akalın Akkök
Alloimmunization against the RhD antigen is the most common cause of hemolytic disease of the fetus and newborn. Antenatal anti-D prophylaxis in addition to postnatal anti-D prophylaxis reduces the number of RhD-immunizations compared to only postnatal administration. Cell-free fetal DNA released from the apoptotic trophoblastic placental cells into the maternal circulation can be used to determine the fetal RHD type in a blood sample from an RhD negative mother. Based on this typing, antenatal anti-D prophylaxis can be recommended only to RhD negative women carrying an RhD positive fetus, since only these women are at risk of developing anti-D...
June 5, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Peter Ridefelt, Linda Hilsted, Anders Juul, Dan Hellberg, Pål Rustad
BACKGROUND: Reference intervals are crucial tools aiding clinicians when making medical decisions. However, for children such values often are lacking or incomplete. The present study combines data from separate pediatric reference interval studies of Denmark and Sweden in order to increase sample size and to include also pre-school children who were lacking in the Danish study. METHODS: Results from two separate studies including 1988 healthy children and adolescents aged 6 months to 18 years of age were merged and recalculated...
May 28, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Caroline U Nilsson, Karin Strandberg, Peter Reinstrup
The anticoagulant warfarin is commonly monitored with prothrombin time (PT). Viscoelastic haemostatic assays (VHA) are primarily used in situations of acute bleeding to guide haemostatic therapy. Much research has focused on VHA monitoring of new oral anticoagulants. However, many patients are still anticoagulated with warfarin and effect of warfarin anticoagulation on VHA is uncertain. The aim of this study was to assess warfarin anticoagulation on three different VHA and compare these findings with prothrombin time (PT), coagulation factor analyses and a thrombin generation assay (TGA)...
May 23, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Benoit Dugué, Giovanni Lombardi, Giuseppe Banfi
Posture changes have been known for a long time to influence the concentration of many analytes in blood especially macromolecules. It is very clear that when someone switches from supine to sitting or standing or from sitting to standing a hemoconcentration is induced. Similarly, when someone switches from standing to sitting or lying a hemodilution occurs. In the context of clinical chemistry, rules have been issued to buffer the impact of postural changes close to the blood specimen collection (e.g. 15 min of seated rest before the blood puncture)...
May 22, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Tarek Mohey El-Gammacy, Dina Mohamed Shinkar, Noha Refaat Mohamed, Amany Rasmy Al-Halag
Preterm neonates with respiratory distress syndrome (RDS) are at increased risk of acute kidney injury (AKI). Our study aimed at determining whether serum cystatin C (sCysC) on day 3 of life (D3) can early predict AKI in preterm neonates with RDS. This prospective study was conducted on 75 preterm neonates; 50 with RDS and 25 without RDS. On D3, sCysC, serum creatinine (sCr) and blood urea nitrogen (BUN) were measured and estimated glomerular filtration rate (eGFR) was calculated. sCr and BUN levels were measured again on days 5 and 7...
May 22, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Mustafa K Özçürümez, Rainer Haeckel
Reference limits (RLs) are required to evaluate laboratory results for medical decisions. The establishment of RL depends on the pre-analytical and the analytical conditions. Furthermore, biological characteristics of the sub-population chosen to provide the reference samples may influence the RL. The most important biological preconditions are gender, age, chronobiological influences, posture, regional and ethnic effects. The influence of these components varies and is often neglected. Therefore, a list of biological variables is collected from the literature and their influence on the estimation of RL is discussed...
May 15, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Freja Eriksen, Jutarat Charoenpatrawut, Sumbul Zaman, Isa Neimann Thomasen, Lise Bathum
No abstract text is available yet for this article.
May 15, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Denis Monneret, Fouzi Mestari, Shaedah Djiavoudine, Guillaume Bachelot, Maxime Cloison, Françoise Imbert-Bismut, Maguy Bernard, Pierre Hausfater, Jean-Marc Lacorte, Dominique Bonnefont-Rousselot
Wide-range C-reactive protein (wr-CRP) has been proposed as an economical alternative to high-sensitivity C-reactive protein (hs-CRP) for the evaluation of low-grade inflammation-associated cardiovascular risk (LGI-CVR). Concomitant values of serum hs-CRP and plasma wr-CRP ≤5 mg/L, and high-sensitivity cardiac troponin T (hs-cTnT), all assayed on Roche Diagnostics analyzers over a 1.8-year period, were extracted from a hospital laboratory database. Hs-CRP and wr-CRP values were compared (Bland-Altman method; Deming's correlation), then separately classified into low (<1 mg/L), moderate (1-3 mg/L) and high (>3 mg/L) LGI-CVR ranges for agreement test (κ), assessed before and after Deming's regression-based adjustment of wr-CRP (Adj-wr-CRP)...
May 15, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Sylwia Płaczkowska, Lilla Pawlik-Sobecka, Izabela Kokot, Agnieszka Piwowar
Civilizational developments occurring during recent decades have resulted in an increased incidence of a variety of metabolic disorders related to insulin resistance in younger people. The determination of decision limits for insulin resistance indices, especially among young people, is a significant challenge in clinical practice. The aim of this study was the estimation of metabolic factors related to their relationship to insulin resistance and metabolic syndrome (MS) features in young, apparently healthy people...
May 9, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Hajime Kataoka
This study examined how changes in heart failure (HF) status induce changes in the index of erythrocyte hydration based on mean red blood cell volume (MCV). Data from 47 HF patients (32% men; 78.2 ± 9.7 years) that experienced worsening and recovery of HF were analysed. Blood tests included measurements of MCV, albumin, serum solutes and b-type natriuretic peptide (BNP). Among a total of 47 worsening HF events, changes in MCV were positively correlated with changes in body weight (r = 0.31, p = ...
April 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Ivan Tasić, Svetlana Kostić, Nikola M Stojanović, Vlado Skakić, Jovana Cvetković, Aleksandar Djordjević, Mirjana Karadzić, Dragan Djordjević, Stanoje Andonov, Viktor Stoičkov, Dimitrije Tasić, Meenakshi Vanka, Dragan Lović
The goal of the present study was to determine the prevalence of hyperuricemia in patients with coronary artery disease (CAD), within three months after coronary events. Also, we aimed to determine whether the presence of hyperuricemia holds correlation with severe CAD, overall heart functioning and risk factors for CAD. The study included 505 consecutive CAD patients, 385 males and 120 females, aged 60.9 ± 9.6 years, with a mean body mass index (BMI) 28.0 ± 3.7 kg/m2 . All patients were admitted to specialized cardiovascular rehabilitation within three months post-acute myocardial infarction (AMI) without revascularization (32...
April 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Qiang Su, Ziliang Ye, Yuhan Sun, Huafeng Yang, Lang Li
To investigate the relationship between miRNA-30e level in circulation and no-reflow phenomenon in patients with acute ST-segment elevation myocardial infarction (STEMI) during primary percutaneous coronary intervention (pPCI). A total of 255 consecutive patients with STEMI undergoing pPCI were enrolled in this study. These patients were divided into two groups according to the occurrence of reflow during pPCI, namely normal-reflow group with 214 cases and no-reflow group with 41 cases. The plasma levels of miRNA-30e were quantified using real-time quantitative polymerase chain reaction...
April 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Anita Rogowicz-Frontczak, Stanislaw Pilacinski, Anna Teresa Chwialkowska, Dariusz Naskret, Dorota Zozulinska-Ziolkiewicz
To investigate the effect of insulin resistance (IR) on thyroid function, thyroid autoimmunity (AIT) and thyroid volume in type 1 diabetes (T1DM). 100 consecutive patients with T1DM aged 29 (±6) years with diabetes duration 13 (±6) years were included. Exclusion criteria were: history of thyroid disease, current treatment with L-thyroxin or anti-thyroid drugs. Evaluation of thyroid stimulating hormone (TSH), free thyroid hormones and anti-thyroid antibodies was performed. Thyroid volume was measured by ultrasonography...
April 19, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Yousun Chung, Dae-Hyun Ko, Jungwon Hyun, Hyun Soo Kim, Min-Jeong Park, Dong Hoon Shin
Urine erythrocyte (Ery) and leukocyte (Leu) dipstick tests are essential for detecting microhematuria and urinary tract infection. Currently, there is no suggestion for establishing the cut-off limits in an ordinal scale test. This study aimed to establish the cut-off limits for urine Ery and Leu dipstick tests via probit regression. From 1 January 2016 to 30 June 2016, laboratory data were collected from patients at one teaching hospital whose specimen had analytical results from both a urine dipstick test and an automated urine particle analyzer...
April 18, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Caio Mauricio Mendes de Cordova, Ernani Tiarajú de Santa Helena, Caroline Galgowski, Vitor Holmo Figueira, Gabrielle Bortoli Setter, Marcello Ricardo Paulista Markus, Carsten Oliver Schmidt, Henry Völzke, Till Ittermann
A simple equation established by Cordova & Cordova (LDL-COR) was developed to provide an improved estimation of LDL-cholesterol in a large Brazilian laboratory database. We evaluated this new equation in a general population cohort in Pomerania, north-eastern Germany (SHIP Study) compared to other existing formulas (Anandaraja, Teerakanchana, Chen, Hattori, Martin, Friedewald and Ahmadi), and its power in the prediction of death by atherosclerosis related events as the primary outcome. Analysis was conducted on a cohort of 4075 individuals considering age, gender, use of lipid lowering therapy and associated co-morbidities such as diabetes, hepatic, kidney and thyroid disease...
May 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Defeng Zhang, Yuanbo Wu, Gengyun Sun
The aim of this study was to investigate the role of miR-192 in differentiation of T follicular helper cells in childhood asthma. Blood samples were taken from eighteen children with acute asthma attacks and fifteen healthy children (HC). Quantitative real-time PCR and Western blotting were used to detect the expression levels of miR-192, C-X-C chemokine receptor type 5 (CXCR5), B-cell lymphoma 6 (BCL-6) and inducible T-cell costimulator (ICOS). The flow cytometry was performed to detect the proportion of CD4 + CXCR5+ Tfh cells on CD4 + T lymphocytes...
May 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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