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Scandinavian Journal of Clinical and Laboratory Investigation

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https://www.readbyqxmd.com/read/28079404/free-amino-acids-in-fibromyalgia-syndrome-relationship-with-clinical-picture
#1
Valeria Ruggiero, Massimiliano Mura, Enrico Cacace, Benedetta Era, Marcella Peri, Giuseppina Sanna, Antonella Fais
The objectives of our study were to evaluate free amino acid (FAA) concentrations in the serum of patients affected by fibromyalgia syndrome (FMS) and to determine the relationships between FAA levels and FMS clinical parameters. Thus, serum amino acid concentrations were quantified (HPLC analysis) in 23 females with fibromyalgia (according to the American College of Rheumatology classification criteria) and 20 healthy females. The results showed significantly higher serum concentrations of aspartate, cysteine, glutamate, glycine, isoleucine, leucine, methionine, ornithine, phenylalanine, sarcosine, serine, taurine, tyrosine and valine in FMS patients vs...
January 12, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28067075/perioperative-humoral-stress-response-to-laparoscopic-versus-open-aortobifemoral-bypass-surgery
#2
Anne H Krog, Per M Thorsby, Mehdi Sahba, Erik M Pettersen, Irene Sandven, Jørgen J Jørgensen, Jon O Sundhagen, Syed S H Kazmi
Minimally invasive surgery seems to reduce hormonal stress response to surgery, but has not previously been examined in major abdominal vascular surgery. Aortic cross-clamping time and operation time is known to be longer in the totally laparoscopic aortobifemoral bypass (LABF) as compared to open aortobifemoral bypass (OABF). The main objective of our study was to measure the hormonal stress response during surgery and aortic cross-clamping in patients undergoing a totally laparoscopic versus an open aortobifemoral bypass...
January 9, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27905214/the-association-of-plasma-oxidative-status-and-inflammation-with-the-development-of-atrial-fibrillation-in-patients-presenting-with-st-elevation-myocardial-infarction
#3
Hasan Aydin Bas, Fatih Aksoy, Atilla Icli, Ercan Varol, Abdullah Dogan, Dogan Erdogan, Ibrahim Ersoy, Akif Arslan, Hatem Ari, Nihal Bas, Recep Sutcu, Mehmet Ozaydin
Atrial fibrillation (AF) is the most common supraventricular arrhythmia following ST elevation myocardial infarction (STEMI). Oxidative stress and inflammation may cause structural and electrical remodeling in the atria making these critical processes in the pathology of AF. In this study, we aimed to evaluate the association between total oxidative status (TOS), total antioxidative capacity (TAC) and high-sensitivity C-reactive protein (hs-CRP) in the development of AF in patients presenting with STEMI. This prospective cohort study consisted of 346 patients with STEMI...
December 1, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27937008/abnormal-leukocyte-scattergrams-and-immature-platelet-fraction-on-sysmex-xn-9000-analyzer-a-new-diagnostic-tool-for-altered-megakaryopoiesis
#4
Sabrina Buoro, Barbara Manenti, Michela Seghezzi, Paola Dominoni, Mauro Vavassori, Rosangela Trezzi, Monica Galli, Guido Finazzi
No abstract text is available yet for this article.
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27937007/hemoglobin-amino-acids-by-the-numbers
#5
Olav Klingenberg
No abstract text is available yet for this article.
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27937006/combination-of-real-time-pcr-and-sequencing-to-detect-multiple-clinically-relevant-genetic-variations-in-the-lactase-gene
#6
Claus Lohman Brasen, Lone Frischknecht, Dorthe Ørnskov, Lotte Andreasen, Jonna Skov Madsen
BACKGROUND: Lactase persistence is an autosomal dominant trait commonly distributed in Europe as well as some parts of east Africa and the Arabian Peninsula. Using real-time PCR to detect the -13910C > T variant common in the European population is a reliable analysis although other variants in the probe-binding site may cause errors in analysis. The aim of this study was to determine the prevalence of the variants in a Danish cohort examined for lactose intolerance as well as to improve the real-time PCR analysis for detection of the different variants...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27905219/early-24-hour-blood-pressure-response-to-roux-en-y-gastric-bypass-in-obese-patients
#7
Julie S Pedersen, Christian Borup, Morten Damgaard, Vindhya D Yatawara, Andrea K Floyd, Niels Gadsbøll, Peter K Bonfils
Recently, it has been proposed, that the blood pressure (BP) lowering effect of gastric bypass surgery not only is explained by the obtained weight loss, but that the anatomical rearrangement of the gut after 'malabsorptive' surgical techniques, such as the laparoscopic Roux-en-Y gastric bypass (LRYGB), may affect BP through a change in a putative 'entero-renal' axis. If so one could anticipate a reduction in BP even before a noticeable weight loss was obtained. The purpose of the present study was to investigate the very early BP response to LRYGB surgery...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27905210/erythrocyte-fatty-acid-composition-does-not-influence-levels-of-free-bioavailable-and-total-25-hydroxy-vitamin-d
#8
Martin Carlsson, Ingela Nilsson, Lars Brudin, Siv-Ping Von, Pär Wanby
In vitro, mono- and polyunsaturated fatty acids (FAs) may decrease the binding affinity of vitamin D metabolites for vitamin D-binding protein, which in turn may influence their bioavailability. FAs incorporated as phospholipids in erythrocyte (ery-) cell membranes reflect dietary intake. The purpose of this study was to investigate ery-FA composition in relation to markers for vitamin D. In healthy females (age 22.6 ± 2.0 years) total 25(OH)D was measured by LC-MS/MS (n = 78), free 25(OH)D with ELISA (n = 64 of 78), and bioavailable 25(OH)D was calculated...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27826984/serum-serotonin-concentration-associated-with-bone-mineral-density-in-chinese-postmenopausal-women
#9
Qiu-Shi Wei, Zhen-Qiu Chen, Xin Tan, Lu-Chen Kang, Xiao-Bing Jiang, Jiang Liang, Wei He, Wei-Min Deng
Recent studies have shown that circulating serotonin plays a potential role in bone metabolism. However, conflicting results have been reported for the relationship between serum serotonin concentrations and bone mineral density (BMD). We investigated whether the serum serotonin concentrations related to BMD in Chinese postmenopausal women. Serum serotonin and bone turnover concentrations of 117 premenopausal women and 262 asymptomatic postmenopausal women were analyzed by enzyme-linked immunosorbent assay...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27768851/analysis-of-hba1c-on-an-automated-multicapillary-zone-electrophoresis-system
#10
Niclas Rollborn, Torbjörn Åkerfeldt, Gunnar Nordin, Xiao Yan Xu, Aleksandra Mandic-Havelka, Lars-Olof Hansson, Anders Larsson
Hemoglobin A1c (HbA1c) is a frequently requested laboratory test and there is thus a need for high throughput instruments for this assay. We evaluated a new automated multicapillary zone electrophoresis instrument (Capillarys 3 Tera, Sebia, Lisses, France) for analysis of HbA1c in venous samples. Routine requested HbA1c samples were analyzed immunologically on a Roche c6000 instrument (n = 142) and then with the Capillarys 3 Tera instrument. The Capillarys 3 Tera instrument performed approximately 70 HbA1c tests/hour...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27762145/elevated-prostate-specific-antigen-and-reduced-10-year-survival-among-a-cohort-of-danish-men-consecutively-referred-from-primary-care-to-an-urological-department-during-2005-2006
#11
Thore Hillig, Torben Kjær Nielsen, Steen Ingemann Hansen, Ann-Britt Nygaard, György Sölétormos
It remains unclear whether total prostate specific antigen (tPSA) or complex PSA (cPSA) has the best diagnostic performance. Additionally, the utility of percentage free PSA (%fPSA) is still debated. Our objectives were to compare the diagnostic performances of tPSA, cPSA, and %fPSA among patients referred from GP to an Urological Specialist and to investigate prognostic factors and survival in the cohort. A total of 1261 consecutive male patients without previously known prostate cancer (PCa) were referred to the same Department of Urology during June 2005 to August 2006...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27762143/diagnostic-value-of-serum-squamous-cell-carcinoma-antigen-for-hepatocellular-carcinoma-a-systematic-review-and-meta-analysis
#12
Jing Yu, Zhao-Juan Wang, Long-Hua Chen, Wen-Zhu Dong
The aim of this study was to ascertain the diagnostic value of serum squamous cell carcinoma antigen (SCCA) and SCCA-IgM for hepatocellular carcinoma (HCC). After a comprehensive search of PubMed and Web of Science databases, we identified eligible studies on the diagnostic value serum SCCAs for HCC. The quality of the eligible studies was assessed using the revised Quality Assessment for Studies of Diagnostic Accuracy (QUADAS-2) tool. The overall diagnostic value of SCCAs for HCC was pooled using a bivariate model...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27758140/association-of-lipoprotein-subfractions-with-endothelial-function-and-arterial-stiffness-in-acute-ischemic-stroke
#13
Pavel Šiarnik, Zuzana Čarnická, Zuzana Krivošíková, Katarína Klobučníková, Ingrid Žitňanová, Branislav Kollár, Marek Sýkora, Peter Turčáni
Hypercholesterolemia represents a risk factor for the development of atherosclerosis. Lipoprotein research has recently been focused on the phenomenon of atherogenic and non-atherogenic lipoproteins. The aim of this study was to explore the association of lipoprotein subfractions with a measure for endothelial function (represented by reactive hyperemia index [RHI]) and arterial stiffness (represented by augmentation index [AI]) in patients with acute ischemic stroke. We enrolled 51 patients with acute ischemic stroke...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27739333/factitious-hyperkalemia-in-hematologic-disorders
#14
Árpád Lábadi, Ágnes Nagy, Árpád Szomor, Attila Miseta, Gábor L Kovács
No abstract text is available yet for this article.
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27724023/coagulopathy-in-patients-with-acute-pulmonary-embolism-a-pilot-study-of-whole-blood-coagulation-and-markers-of-endothelial-damage
#15
Per Lehnert, Pär I Johansson, Sisse R Ostrowski, Christian H Møller, Lia E Bang, Peter Skov Olsen, Jørn Carlsen
Whole blood coagulation and markers of endothelial damage were studied in patients with acute pulmonary embolism (PE), and evaluated in relation to PE severity. Twenty-five patients were enrolled prospectively each having viscoelastical analysis of whole blood done using thrombelastography (TEG) and Multiplate aggregometry. Fourteen of these patients were investigated for endothelial damage by ELISA measurements of Syndecan-1 (endothelial glycocalyx degradation), soluble endothelial Selectin (endothelial cell activation), soluble Thrombomodulin (endothelial cell injury) and Histone Complexed DNA fragments (endothelial cytotoxic histones)...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27191339/can-the-afinion-hba1c-point-of-care-instrument-be-an-alternative-method-for-the-tosoh-g8-in-the-case-of-hb-tacoma
#16
Erna Lenters-Westra, Annuska Strunk, Paul Campbell, Robbert J Slingerland
BACKGROUND: Hb-variant interference when reporting HbA1c has been an ongoing challenge since HbA1c was introduced to monitor patients with diabetes mellitus. Most Hb-variants show an abnormal chromatogram when cation-exchange HPLC is used for the determination of HbA1c. Unfortunately, the Tosoh G8 generates what appears to be normal chromatogram in the presence of Hb-Tacoma, yielding a falsely high HbA1c value. The primary aim of the study was to investigate if the Afinion HbA1c point-of-care (POC) instrument could be used as an alternative method for the Tosoh G8 when testing for HbA1c in the presence of Hb-Tacoma...
February 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27739331/large-scale-performance-evaluation-of-accu-chek-inform-ii-point-of-care-glucose-meters
#17
Tae-Dong Jeong, Eun-Jung Cho, Dae-Hyun Ko, Woochang Lee, Sail Chun, Ki-Sook Hong, Won-Ki Min
BACKGROUND: The aim of this study was to report the experience of large-scale performance evaluation of 238 Accu-Chek Inform II point-of-care (POC) glucose meters in a single medical setting. METHODS: The repeatability of 238 POC devices, the within-site imprecision of 12 devices, and the linearity of 49 devices were evaluated using glucose control solutions. The glucose results of 24 POC devices and central laboratory were compared using patient samples. RESULTS: Mean concentration of control solutions was 2...
December 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27712122/the-relationship-between-circulating-visfatin-nicotinamide-phosphoribosyltransferase-obesity-inflammation-and-lipids-profile-in-elderly-population-determined-by-structural-equation-modeling
#18
Aleksander J Owczarek, Magdalena Olszanecka-Glinianowicz, Piotr Kocełak, Maria Bożentowicz-Wikarek, Aniceta Brzozowska, Małgorzata Mossakowska, Monika Puzianowska-Kuźnicka, Tomasz Grodzicki, Andrzej Więcek, Jerzy Chudek
BACKGROUND: The available literature suggests that circulating visfatin/Nicotinamide Phosphoribosyltransferase (NAMPT) level variability in humans is related to obesity, insulin resistance, inflammation, and lipid profile. The aim of the study was to assess the relationship between circulating visfatin/NAMPT, obesity, insulin resistance, inflammation, and lipid profile in a large population-based, elderly cohort, applying structural equation modeling. MATERIALS AND METHODS: The analysis included 2983 elderly participants of the PolSenior study with assessed total blood count, fasting concentrations of lipids, glucose, insulin, hs-CRP, interleukin-6, and visfatin/NAMPT (by ELISA), and calculated HOMA-IR...
December 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27705007/the-variation-in-high-sensitive-cardiac-troponin-concentration-during-haemodialysis-treatment-is-not-similar-to-the-biological-variation-observed-in-stable-end-stage-renal-disease-patients
#19
Øyvind Skadberg, Sverre Sandberg, Thomas Røraas, Per Hyltoft Petersen, Hilde Sellevoll, Einar Svarstad, Kristin Sæle, Kristin Moberg Aakre
BACKGROUND: Cardiovascular mortality is high in end stage renal disease (ESRD). This study aimed to: (1) calculate within-week within- and between-subject biological variation (CVI and CVG) for hs-cTn in ESRD; (2) determine the magnitude of hs-cTn concentration changes during haemodialysis (HD) treatment; and (3) compare the CVI and CVG to the within and between-subject variation of cTn concentration changes during HD treatments (CVDIFF-I and CVDIFF-G). METHODS: Serum samples were collected from 20 patients before and after 10 consecutive HD treatments...
December 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27701904/impedance-aggregometric-analysis-of-platelet-function-of-apheresis-platelet-concentrates-as-a-function-of-storage-time
#20
Michael Glas, Janine Viola Bauer, Hermann Eichler, Thomas Volk
Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10(-9 )L and a calcium concentration of 5 mmol L(-1)...
December 2016: Scandinavian Journal of Clinical and Laboratory Investigation
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