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Scandinavian Journal of Clinical and Laboratory Investigation

Pablo Corres, Sara Maldonado-Martín, Ilargi Gorostegi-Anduaga, Simon M Fryer, Borja Jurio-Iriarte, Aitor MartínezAguirre-Betolaza, Iñaki Arratibel-Imaz, Silvia Francisco-Terreros, Javier Pérez-Asenjo
Cardiorespiratory fitness (CRF) is positively associated with enhanced cardiovascular health. This cross-sectional study aimed to determine associations between CRF and the biochemical profile of overweight/obese adults diagnosed with primary hypertension (HTN). Does cardiorespiratory fitness (exposure) positively affect the biochemical profile (outcome) in overweight/obese individuals suffering from HTN? Assessment with anthropometric, ambulatory blood pressure monitoring (24 h), CRF (peak oxygen uptake, V̇O2peak ) and biochemical analysis was performed on 214 participants (138 men, 76 women)...
November 24, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Josef van Helden, Ralf Weiskirchen
There is growing interest in measuring plasma fibroblast growth factor 23 (FGF23) concentrations in a number of clinical settings. However, a reliable assay with acceptable performance is lacking. Plasma samples of healthy adults and patients with different stages of chronic kidney disease (CKD) were used to compare the precision, recovery, linearity and the pre-analytical stability characteristics of a new fully automated FGF23 (intact) assay with a commercially available FGF23 (intact) ELISA. Method agreement was evaluated, reference and stage-specific ranges for kidney disease were established...
October 31, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Anping Xu, Jie Lei, Jie Li, Weidong Chen, Yu Zhou, Ruoyang Zheng, Ling Ji
Hemoglobin A1c (HbA1c ) is a widely utilized biomarker for the diagnosis and management of diabetes mellitus. Here, we describe an α1-globin chain hemoglobin variant and investigate its effect on HbA1c measurement. A 26-year-old pregnant woman was suspected to harbor a hemoglobin variant following HbA1c measurement during a routine prenatal examination using D10 (Bio-Rad). An oral glucose tolerance test (OGTT) was performed using an AU5800 clinical chemistry system (Beckman Coulter). HbA1c was reanalyzed using VII-T 2...
October 30, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Jinyoung Yang, Hyeyoung Lee, Ae Ran Choi, Ki Hyun Park, Ji Hyeong Ryu, Eun-Jee Oh
In vitro allergen-specific immunoglobulin E (sIgE) measurement has been used as an important diagnostic tool for allergic diseases. Currently, quantitative sIgE levels are detected mainly by using ImmunoCAP and Immulite 2000 assay system. These two systems have the same calibration scale at 0-100 kUA/L, but they differ in used allergens, detection methods and automation systems. We compared 1600 paired sIgE results for 204 allergic patients, including 100 paired sIgE assay results for each of 16 allergens (Alternaria alternata, birch-alder mix, cat dander, D...
October 30, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Nicoleta Răcătăianu, Nicoleta Valentina Leach, Sorana D Bolboacă, Angela Cozma, Eleonora Dronca, Ana Valea, Alina Silaghi, Ana Maria Bîlc, Cristina Ghervan
The aim of this article is to study the possible relation of serum vitamin D concentrations to body mass index (BMI), visceral fat thickness (VFT), insulin resistance (IR), inflammation (serum monocyte chemoattractant protein-1 - MCP-1) and thyroid parameters in obese patients. A total of 158 non-diabetic, obese patients aged 19-68 without a history of thyroid pathology were recruited. Biochemical markers, insulin, 25-OH vitamin D, thyroid parameters (TSH, FT3, FT4, TPO antibodies, TG antibodies) and VFT were measured...
October 26, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Paul Guenther Lassmann-Klee, Lauri Lehtimäki, Tuula Lindholm, L Pekka Malmberg, Anssi Raimo Antero Sovijärvi, Päivi Piirilä
Fractional exhaled nitric oxide (FENO ) is used to assess eosinophilic inflammation of the airways. FENO values are influenced by the expiratory flow rate and orally produced NO. We measured FENO at four different expiratory flow levels after two different mouthwashes: tap water and carbonated water. Further, we compared the alveolar NO concentration (CANO ), maximum airway NO flux (J'awNO ) and airway NO diffusion (DawNO ) after these two mouthwashes. FENO was measured in 30 volunteers (healthy or asthmatic) with a chemiluminescence NO-analyser at flow rates of 30, 50, 100 and 300 mL/s...
October 22, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Kjell Grankvist, Gudmundur Sigthorsson, Gunn B Kristensen, Jonna Pelanti, Mads Nybo
The preanalytical phase contains a vast number of practices whose variation may influence the results of laboratory testing and should, therefore, be standardized. The Working Group on Preanalytical Phase of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM WG-PA) has suggested a standardization of venous blood specimen collection (VBSC) requirements for fasting samples including 12 h fasting time and water ad lib in the morning prior to specimen collection. The Nordic Scientific Preanalytical Working Group investigated the fasting definitions used in the Nordic countries...
October 19, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Astrid H Egesborg, Henrik Sørensen, Niels D Olesen, Niels H Secher
Phenylephrine is an α-adrenergic agent and yet seems to increase near-infrared spectroscopy determined muscle oxygenation (Sm O2 ) that reflects the ratio between oxygenated (O2 Hb) and deoxygenated (Hb) haemoglobin/myoglobin. We examined whether the increase in Sm O2 by phenylephrine reflects veno-constriction and to secure filling of the veins, subjects were exposed to head-up tilt (HUT). Phenylephrine (0.2 mg) was administered to 10 healthy males (24 years (22-27; median with interquartile range)) during 40° HUT with Sm O2 , O2 Hb and Hb determined for the biceps brachii and vastus lateralis muscles...
October 19, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Andreas Hillarp, Karin Strandberg, Fariba Baghaei, Inger Fagerberg Blixter, Kerstin M Gustafsson, Tomas L Lindahl
Edoxaban is an oral direct factor Xa inhibitor for prophylaxis and treatment of thromboembolic disorders. The effects on common coagulation assays are clinically valuable information and in certain clinical situations a quick assessment of the anticoagulant is wanted. Our aim was to investigate the effect of edoxaban on routine coagulation methods and evaluate anti-Xa assays, commonly used for other direct factor Xa inhibitors, for estimation of the drug concentration. Edoxaban was spiked to plasma samples from healthy subjects in the concentration range 0-742 µg/L and analyzed using different reagents for activated partial thromboplastin time (APTT) and prothrombin time (PT)...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Marevic Sanja, Petrik Jozsef, Popovic Grle Sanja, Cepelak Ivana, Grgic Ivana, Gorenec Lana, Stjepanovic Gordana, Laskaj Renata, Zidovec Lepej Snjezana
Cytokines are biological response modifiers involved in the pathophysiology of chronic obstructive pulmonary disease (COPD). This study investigated the potential use of cytokines as disease severity biomarkers in COPD patients and the possible effect of statin therapy on cytokine expression. Possible associations between cytokines, body mass index (BMI) and smoking have also been studied. Cytokines IFN-γ, IL-2, IL-12 p70, TNF-α, TNF-β, IL-4, IL-5, IL-6, IL-10, IL-1β and IL-8 were measured in the plasma of 100 clinically stable COPD patients using a fluorescent bead immunoassay on a flow cytometer...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Aline Louise Lemes, Josi Karla Amadeu, Cristina Salazar Berrocal, Juliana Lucena Schussel, Meltem Bakkal, José Miguel Amenábar
Oxidative stress biomarkers of oral and systemic diseases can be found in saliva. However, there is no uniformity for the saliva collection time in these kinds of analyses and saliva composition may change because of mechanical stimulation. Therefore, the aim of this work was to determine the effect of mechanical stimulation for 10 min on the concentrations of vitamin C, vitamin E, total antioxidants and total oxidants in saliva. Saliva samples from individuals of both sexes, aged between 18 and 38 years, were collected for 10 min at 2 minintervals...
October 2, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Ilya Valerevich Smirnov, Irina Vladimirovna Gryazeva, Margarita Yurevna Vasileva, Irina Yurevna Krutetskaia, Olga Alexandrovna Shashkova, Marina Platonovna Samoylovich, Anastasia Yurevna Stolbovaya, Nellya Grigorevna Solodovnikova, Irina Evegnevna Zazerskaya, Dmitriy Igorevich Sokolov, Sergey Alexeevich Selkov, Vladimir Borisovich Klimovich
Soluble endoglin (sEng) is a fragment of a membrane-associated receptor (CD105) expressed on endothelial cells, mesenchymal stem cells and trophoblast cells. It is considered as a regulatory factor involved in the development of preeclampsia (PE) and cancer-associated neo-angiogenesis. The purpose of this study was to describe a new sandwich ELISA for sEng quantification. In contrast to three commercial kits, the new ELISA was able to quantify sEng not only in blood plasma and cell culture supernatants, but also in urine and cerebrospinal fluid...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Magnus H Jonsson, Ami Hommel, Aleksandra Turkiewicz, Ulf Ekelund, Olle Melander, Martin Englund, Peter Bentzer
Hip fractures in elderly carry a high mortality. Our objective was to test the hypothesis that plasma lactate concentration at hospital admission can be used to identify patients with a high risk for poor outcome. Hip fracture patients admitted to a university hospital in Sweden from January 2011 to August 2014 in whom a venous lactate was obtained at admission were included in this prospective observational study. Primary outcome measure was 30-d mortality and secondary outcome measure was a composite outcome of 30-d mortality and postoperative complications...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Dae-Hyun Ko, Eun-Jung Cho, Woochang Lee, Sail Chun, Won-Ki Min
Therapeutic drug monitoring of tacrolimus and cyclosporine is crucial to the success of organ transplantation. We evaluated the analytical performances and accuracy of two commercially available tacrolimus and cyclosporine assays (Roche ISD and Siemens) in comparison with liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 342 leftover whole blood samples requested for tacrolimus or cyclosporine assays were stored at -20 °C until analysis. Repeatability and between-run imprecision were evaluated using quality control materials provided by the manufacturer...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
August Bagge, Ulf Schött, Thomas Kander
The effect of omega-3 fatty acids on platelet aggregation and coagulation is highly unclear. Studies both support and refute the impacts of omega-3 fatty acids on prolonged bleeding time and platelet inhibition as well as its purported positive effects on cardiovascular disease. In a previous pilot study we suggested an inhibition of platelet aggregation measured with multiple electrode aggregometry. Following on that, the aim of the present study was to investigate the effects of supplementary high doses of omega-3 fatty acids on platelet aggregation and coagulation in a sample-size calculated number of healthy volunteers using Sonoclot, multiple electrode aggregometry, and flow-based Cellix instruments after 10 days of omega-3 fatty acid intake...
October 1, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Eun Hye Cho, Rihwa Choi, Eun-Suk Kang, Hyung-Doo Park
IgG consists of four subclasses: IgG1, IgG2, IgG3, and IgG4. Changes in the serum concentration of each subclass reflect different clinical situations, and quantification of each subclass is important to assess patients' clinical states. Herein, we evaluated the analytical performance of the SPAPLUS turbidimetric analyzer (The Binding Site, Birmingham, UK) for IgG subclass. Precision, linearity, comparison with the BNII system (Siemens Healthineers, Erlangen, Germany), and reference interval were assessed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Sofie Sommer Hedegaard, Anna Christina Klein, Anne-Mette Hvas
The role of thrombophilia in paediatric thromboembolic disease remains controversial. The objective of the present study was to investigate the prevalence of thrombophilia and other risk factors among children with a thromboembolic event. We systematically examined the medical records and laboratory results of 126 children (≤15 years) referred to Aarhus University Hospital, Denmark with a recent thromboembolic event during 2005-2016. Among the 126 children with thrombosis, 99 (79%) had arterial thrombosis, 66 (52%) being in the neonatal period...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Erland Jørn Erlandsen, Else Randers
The aim of this study was to document the differences between two Roche creatinine measurement methods, the CREP2 test on the cobas c702 and the CREA PLUS test on the Modular P. Samples with creatinine isotope dilution mass spectrometry (IDMS) values were analyzed on both instruments. Method comparison using the remaining plasma samples was performed twice, using two different lot numbers of reagents and two different lot numbers of calibrators on both instruments. Medians and percentiles of the plasma creatinine values produced on the Modular P and cobas c702 from 2012 to 2017 were compared...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Bodil Bjørndal, Inge Bruheim, Vegard Lysne, Marie S Ramsvik, Per M Ueland, Jan E Nordrehaug, Ottar K Nygård, Rolf K Berge
Plasma concentrations of metabolites along the choline oxidation and tryptophan degradation pathways have been linked to lifestyle diseases and dietary habits. This study aimed to investigate how krill oil, a source of ω-3 polyunsaturated fatty acids (PUFAs) with a high phosphatidylcholine content, affected these parameters. The pilot study was conducted as a 28 days intervention in 17 healthy volunteers (18-36 years), who received a supplement of 4.5 g krill oil per day, providing 833 mg ω-3 PUFAs, and 1750 mg phosphatidylcholine...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Songlin Yu, Yicong Yin, Qian Cheng, Jianhua Han, Xinqi Cheng, Ye Guo, Dandan Sun, Shaowei Xie, Ling Qiu
Rapid and simple method for serum and urine iodine is necessary for iodine-related diseases. This study aimed to establish and validate a simple and rapid method for quantification of iodine in human urine and serum using inductively coupled plasma mass spectrometry (ICP-MS). Generally, samples were diluted using water with 1.5% isopropanol and 7 mmol hydrous ammonium before analysis by the ICP-MS. Performance of the method was validated, and the urine and serum iodine levels of 72 pregnant women were analyzed...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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