Marcos Luis-Calero, Federica Marinaro, Pablo Fernández-Hernández, José M Ortiz-Rodríguez, Javier G Casado, Eva Pericuesta, Alfonso Gutiérrez-Adán, Esperanza González, Mikel Azkargorta, Ricardo Conde, Maider Bizkarguenaga, Nieves Embade, Félix Elortza, Juan M Falcón-Pérez, Óscar Millet, Lauro González-Fernández, Beatriz Macías-García
In vitro maturation (IVM) of oocytes is clinically used in horses to produce blastocysts but current conditions used for horses are suboptimal. We analyzed the composition of equine preovulatory follicular fluid (FF) secretome and tested its effects on meiotic competence and gene expression in oocytes subjected to IVM. Preovulatory FF was obtained, concentrated using ultrafiltration with cut-off of 10 kDa, and stored at -80 °C. The metabolic and proteomic composition was analyzed, and its ultrastructural composition was assessed by cryo-transmission microscopy...
March 11, 2024: Research in Veterinary Science