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Research in Veterinary Science

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https://www.readbyqxmd.com/read/29793151/effect-of-two-months-whole-body-vibration-on-hoof-growth-rate-in-the-horse-a-pilot-study
#1
Bart Tom Halsberghe
Hoof problems are commonly seen in veterinary practice and manipulation of hoof growth rate can be practical and beneficial for that matter. The purpose of this research was to evaluate the effect of whole body vibration (WBV) on hoof growth rate of front feet in the horse. The study was an experimental, single subject, repeated measure design, with all horses serving as control and treatment. Ten horses were subject to WBV, 30 min, twice daily, five days a week, for 60 days in addition to their regular exercise routine...
May 16, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29783122/bix-01294-promotes-the-differentiation-of-adipose-mesenchymal-stem-cells-into-adipocytes-and-neural-cells-in-arbas-cashmere-goats
#2
Qing Wang, Xiao Wang, Defang Lai, Jin Deng, Zhuang Hou, Hao Liang, Dongjun Liu
Chromatin remodeling plays an essential role in regulating gene transcription. BIX-01294 is a specific inhibitor of histone methyltransferase G9a, which is responsible for methylation of histone H3 lysine 9 (H3K9) that can also regulate DNA methylation and chromatin remodeling. The purpose of this study was to investigate the effects of BIX-01294 on the potential of goat adipose derived stem cells (gADSCs) to differentiate into adipocytes and neural cells. To accomplish this, BIX-01294 was used to treat gADSCs for 24 h, and the global level of DNA methylation as well as the expression of genes related to cell proliferation, apoptosis and pluripotency were detected...
May 14, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29783120/canine-mesenchymal-stem-cells-treated-with-tnf-%C3%AE-and-ifn-%C3%AE-enhance-anti-inflammatory-effects-through-the-cox-2-pge-2-pathway
#3
Hye-Mi Yang, Woo-Jin Song, Qiang Li, Su-Yeon Kim, Hyeon-Jin Kim, Min-Ok Ryu, Jin-Ok Ahn, Hwa-Young Youn
Mesenchymal stem cells (MSCs) have been used in studies on treatment of various diseases, and their application to immune-mediated diseases has garnered interest. Various methods for enhancing the immunomodulation effect of human MSCs have been used; however, similar approaches for canine MSCs are relatively unexplored. Accordingly, we evaluated immunomodulatory effects and mechanisms in canine MSCs treated with TNF-α and IFN-γ. Lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were incubated with the conditioned media (CM) from canine MSCs for 48 h...
May 14, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29758535/on-farm-intervention-studies-on-reduction-of-boar-taint-prevalence-feeding-strategies-presence-of-gilts-and-time-in-lairage
#4
E Heyrman, S Millet, F A M Tuyttens, B Ampe, S Janssens, N Buys, J Wauters, L Vanhaecke, M Aluwé
One of the challenges in the production of entire male pigs is the occurrence of boar taint. We separately tested the effect of 3 management strategies to reduce boar taint on respectively 2, 3, and 6 Flemish pig farms: 1) adapted feeding strategies, 2) presence of gilts in the compartment, and 3) varying lairage duration at the slaughterhouse. A commercialized feed concept resulted in a significant reduction of olfactory boar taint prevalence when fed for 2 weeks (T2W) compared to control (T-CON) (P = 0...
May 9, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29758536/characterization-of-sesec_01411-as-a-surface-protective-antigen-of-streptococcus-equi-ssp-zooepidemicus
#5
Honglin Xie, Zigong Wei, Chunquan Ma, Shun Li, Xiaohong Liu, Qiang Fu
Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is a commensal bacterium related to opportunistic infections of many species, including humans, dogs, cats, and pigs. SeseC_01411 has been proven to be immunogenic. However, its protective efficacy remained to be evaluated. In the present study, the purified recombinant SeseC_01411 could elicit a strong humoral antibody response and protect against lethal challenge with virulent SEZ in mice. Our finding confirmed that SeseC_01411 distributes on the surface of SEZ...
May 8, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29758534/investigations-of-the-corneal-epithelium-in-veterinary-medicine-state-of-the-art-on-corneal-stem-cells-found-in-different-mammalian-species-and-their-putative-application
#6
REVIEW
M Patruno, A Perazzi, T Martinello, C Gomiero, L Maccatrozzo, I Iacopetti
The existence of progenitor cells that can readily differentiate into a specific cell type is a common cellular strategy for physiological tissue growth and repair mechanisms. In the mammalian cornea, many aspects regarding the nature and location of these cells are still unclear. In the human limbus (peripheral area of the cornea) progenitor cells have been found and characterized but in non-human mammals, the picture is not so clear. In this review, we examine current knowledge about the morphology of limbus and the localization of corneal epithelial stem cells in all species studied so far, comparing data with humans...
May 8, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29783121/the-influence-of-nail-blocking-conditions-in-cattle-femoral-fractures
#7
Leopoldo A Paolucci, Estevam B Las Casas, Rafael R Faleiros, Cahuê F R Paz, Sergio S Rocha Junior
BACKGROUND: To investigate the effect of different fixation strategies of the intramedullary interlocking nail (IIN) on the mechanical behavior of a polymeric implant applied for femoral fracture fixation in calves, and to evaluate the performance of a glass fiber-reinforced polymer applied in a bovine femoral fracture reduction system, five Holstein male animals with a mean weight (±SD) of 62.8 ± 20.4 kg and aged 74 ± 15 were used to generate biomechanical parameters for this study...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29775839/veterinary-applications-of-pulsed-electromagnetic-field-therapy
#8
REVIEW
James S Gaynor, Sean Hagberg, Blake T Gurfein
Pulsed electromagnetic field (PEMF) therapy can non-invasively treat a variety of pathologies by delivering electric and magnetic fields to tissues via inductive coils. The electromagnetic fields generated by these devices have been found to affect a variety of biological processes and basic science understanding of the underlying mechanisms of action of PEMF treatment has accelerated in the last 10 years. Accumulating clinical evidence supports the use of PEMF therapy in both animals and humans for specific clinical indications including bone healing, wound healing, osteoarthritis and inflammation, and treatment of post-operative pain and edema...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29758533/antimicrobial-resistance-biofilm-formation-and-virulence-reveal-actinobacillus-pleuropneumoniae-strains-pathogenicity-complexity
#9
Monalessa Fábia Pereira, Ciro César Rossi, Larissa Eler Seide, Sebastião Martins Filho, Cláudia de Melo Dolinski, Denise Mara Soares Bazzolli
Porcine pleuropneumonia is an important cause of lowered productivity and economic loss in the pig industry worldwide, associated primarily with Actinobacillus pleuropneumoniae infection. Its colonization and persistence within the upper respiratory tract of affected pigs depends upon interactions between a number of genetically controlled virulence factors, such as pore-forming repeats-in-toxin exoproteins, biofilm formation, and antimicrobial resistance. This study investigated correlations between biofilm-forming capacity, antimicrobial resistance, and virulence of A...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29758532/evaluation-of-the-nova-vet-meter-for-sheep-side-monitoring-of-%C3%AE-hydroxybutyric-acid-bhba-and-description-of-ewe-bhba-during-late-gestation-in-three-flocks-from-the-northeastern-u-s
#10
Amanda K Jones, Rachael E Gately, Tasia D Kellogg, Steven A Zinn, Kristen E Govoni, Sarah A Reed
Prevention of metabolic diseases in small ruminants may improve production efficiency and profitability, yet ewes carrying multiples or who are in poor body condition are at increased susceptibility to develop ketosis. This study evaluated the hand-held Nova Vet Meter to accurately detect β-hydroxybutyric acid (BHBA) concentrations in ewes and determined the percentage of ewes at moderate (0.8 to 1.5 mmol/L BHBA) and greatest (≥1.6 mmol/L BHBA) risk to develop ketosis during late gestation. To validate the Nova Vet Meter, BHBA concentrations of 104 paired blood samples were measured using the Nova Vet Meter and gold-standard laboratory analysis...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29747134/a-polyclonal-antibody-against-extracellular-loops-1-of-chnhe1-blocks-avian-leukosis-virus-subgroup-j-infection
#11
Wei Meng, Defang Zhou, Chengui Li, Guihua Wang, Libo Huang, Ziqiang Cheng
Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and other various tumors, leading to great economical losses in poultry industry. It is a great challenge to develop effective preventive methods for ALV-J control due to its antigenic variations in the variable regions of envelope. In present study, we generated a mouse polyclonal antibody targeting the first extracellular loop (ECL1) of chicken Na+ /H+ exchanger isoform 1 (chNHE1), the receptor of ALV-J, to block ALV-J infection in vitro and in vivo...
May 2, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29747133/time-dependent-changes-in-gene-expression-induced-in-vitro-by-interleukin-1%C3%AE-in-equine-articular-cartilage
#12
Maria Löfgren, Emilia Svala, Anders Lindahl, Eva Skiöldebrand, Stina Ekman
Osteoarthritis is an inflammatory and degenerative joint disease commonly affecting horses. To identify genes of relevance for cartilage pathology in osteoarthritis we studied the time-course effects of interleukin (IL)-1β on equine articular cartilage. Articular cartilage explants from the distal third metacarpal bone were collected postmortem from three horses without evidence of joint disease. The explants were stimulated with IL-1β for 27 days and global gene expression was measured by microarray. Gene expression was compared to that of unstimulated explants at days 3, 9, 15, 21 and 27...
May 1, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29730246/application-of-biosensors-to-detection-of-epidemic-diseases-in-animals
#13
REVIEW
Xin Du, Jun Zhou
Epidemic diseases are the leading cause of animal mortality, resulting in significant losses to the agricultural economy. These economic impacts have generated a strong interest in advancing methods for the diagnosis and control of epidemic diseases in animals. Conventional methods are often time-consuming (typically result is available in 2-10 days), expensive, and require both large-scale equipment and experienced personnel. However, the advent of biosensor technology has ushered in a new and promising approach for the diagnosis of animal diseases...
April 27, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29751280/evaluation-of-serum-insulin-like-growth-factor-1-and-26s-proteasome-concentrations-in-healthy-dogs-and-dogs-with-chronic-diseases-depending-on-body-condition-score
#14
Ingrid Gerke, Franz-Josef Kaup, Stephan Neumann
In patients suffering from chronic diseases, the objective assessment of metabolic states could be of interest for disease prognosis and therapeutic options. Therefore, the aim of this study was to assess insulin-like growth factor-1 (IGF-1) and 26S proteasome (26SP) in healthy dogs and dogs suffering from chronic diseases depending on their body condition score (BCS) and to examine their potential for objective assessment of anabolic and catabolic states. Serum concentrations of IGF-1, an anabolic hormone, and 26SP, a multiprotein complex which is part of the ubiquitin-proteasome pathway, by which the majority of endogenous proteins including the muscle proteins are degraded, were measured in 21 healthy dogs and 20 dogs with chronic diseases by canine ELISA...
April 26, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29734122/reference-1d-and-2d-electrophoresis-maps-for-potential-disease-related-proteins-in-milk-whey-from-lactating-buffaloes-and-blood-serum-from-buffalo-calves-water-buffalo-bubalus-bubalis
#15
André M Santana, Funmilola C Thomas, Daniela G Silva, Eilidh McCulloch, Ana M C Vidal, Richard J S Burchmore, José J Fagliari, Peter D Eckersall
The aim of this study was to identify potential disease related proteins in milk whey of lactating buffaloes and blood serum of buffalo calves, in order to define a reference electrophoresis map for 1-DE and 2-DE. Additionally, changes in some protein patterns from buffalo calves during salmonellosis and lactating buffaloes during mastitis are presented. Milk samples were collected and distributed into groups: Milk samples from healthy buffaloes (SCC < 100.000 cells/ml, negative microbiology and CMT) (G1, n = 5) and buffaloes with subclinical mastitis (SCC > 500...
April 26, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29729606/evaluation-of-tissue-levels-of-toll-like-receptors-and-cytokine-mrnas-associated-with-bovine-periodontitis-and-oral-health
#16
Ana C Borsanelli, David F Lappin, Lorenzo Viora, George King, David Bennett, Iveraldo S Dutra, Marcello P Riggio
Bovine periodontitis is a progressive and purulent infection associated with an anaerobic subgingival biofilm, which induces irreversible damage to the dentition of affected animals. The aetiopathogenesis of the disease is unclear and treatment and control of the disease process in cattle are almost unknown. The aim of this study was to investigate the innate immune response by quantifying expression of Toll-like receptor (TLR) and cytokine genes in gingival tissue samples from cattle with and without periodontitis...
April 25, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29698903/fucoidan-suppresses-excessive-phagocytic-capacity-of-porcine-peripheral-blood-polymorphonuclear-cells-by-modulating-production-of-tumor-necrosis-factor-alpha-by-lipopolysaccharide-stimulated-peripheral-blood-mononuclear-cells
#17
Hyeong-Mok Kim, Changhwan Ahn, Byeong-Teck Kang, Ji-Houn Kang, Eui-Bae Jeung, Mhan-Pyo Yang
We examined the effect of fucoidan, an immune modulator, on the phagocytic capacity of porcine peripheral blood polymorphonuclear cells (PMNs) exposed to culture supernatant from lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). For this purpose, we evaluated the phagocytic capacity of porcine PMNs by flow cytometry and measured levels of tumor necrosis factor-alpha (TNF-α) protein and mRNA in porcine PBMCs by enzyme-linked immunosorbent assay (ELISA) and real time-polymerase chain reaction (PCR), respectively...
April 18, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29684816/gaba-regulates-the-proliferation-and-apoptosis-of-mac-t-cells-through-the-lps-induced-tlr4-signaling-pathway
#18
Yue-Ying Wang, Shi-Ping Sun, He-Shui Zhu, Xian-Qin Jiao, Kai Zhong, Yu-Jie Guo, Guang-Ming Zha, Li-Qiang Han, Guo-Yu Yang, He-Ping Li
Subacute ruminal acidosis (SARA) can cause rapid lipopolysaccharide (LPS) elevation and milk yield decline in lactating ruminants. LPS has been shown to promote apoptosis and reduce the proliferation of mammary epithelial cells. Previous studies have shown that γ- amino butyric acid (GABA) can enhance production performance, regulating β-cell apoptosis and proliferation. Whether GABA can regulate apoptosis and proliferation induced by LPS in mammary epithelial cells is unknown. In this paper, we detected the role of GABA on proliferation and apoptosis as well as inflammation induced by LPS in bovine mammary epithelial cells (MAC-T cell line)...
April 18, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29729605/ethanolic-extract-ocimum-sanctum-enhances-cognitive-ability-from-young-adulthood-to-middle-aged-mediated-by-increasing-choline-acetyl-transferase-activity-in-rat-model
#19
Dwi Liliek Kusindarta, Hevi Wihadmadyatami, Arvendi R Jadi, Srikanth Karnati, Guenter Lochnit, Puspa Hening, Aris Haryanto, Made B Auriva, Medania Purwaningrum
Patients with dementia are increasing steadily, cognitive impairment by dementia not only exclusively suffers by old people but also young to middle aged individuals. However, the mechanism of cognitive impairment occurs in young people is not understood. Further, current medication to impairment did not provide satisfactory results. Therefore, we investigated the potential role of Ocimum sanctum ethanolic extract to enhance cognitive ability in the rat in vivo model. Young to middle aged rats were divided into 3 groups (3, 6, 9 months old) were treated with (0, 50 and 100 mg/kg b...
April 16, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29684817/avermectin-toxicity-in-bovines-less-than-thirty-days-old
#20
Daniel de Castro Rodrigues, Carolina Buzullini, Tiago Arantes Pereira, Breno Cayeiro Curz, Lucas Vinicius Costa Gomes, Vando Edésio Soares, Thiago Souza Azeredo Bastos, Luiz Fellipe Monteiro Couto, Welber Daniel Zanetti Lopes, Gilson Pereira de Oliveira, Alvimar José da Costa
The present study was designed to evaluate possible adverse effects of different dosages of avermectins (abamectin and a combination of ivermectin + abamectin) administered subcutaneously in calves less than one month of age. Clinical as well as biochemical parameters of blood and cerebrospinal fluid (CSF) were evaluated for animals subjected to various treatments. Thirty-five calves were divided into seven groups of five animals each: T01 (control), T02 (abamectin 200 μg/kg), T03 (abamectin 400 μg/kg), T04 (abamectin 600 μg/kg), T05 (ivermectin 450 μg/kg + abamectin 250 μg/kg), T06 (ivermectin 900 μg/kg + abamectin 500 μg/kg) and T07 (ivermectin 1350 μg/kg + abamectin 750 μg/kg)...
April 16, 2018: Research in Veterinary Science
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