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Research in Veterinary Science

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https://www.readbyqxmd.com/read/29132685/first-report-on-the-isolation-of-trueperella-abortisuis-from-companion-animals
#1
J-P Wickhorst, A A Hassan, O Sammra, M Alssahen, C Lämmler, E Prenger-Berninghoff, M Naggert, M Timke, J Rau, A Abdulmawjood
The present study gives a detailed phenotypic and genotypic characterization of three Trueperella abortisuis strains isolated from a ten year old male Hovawart dog with an abscess of anal sac, from urine of an eight year old European shorthair cat with urolithiasis and nephrolithiasis and from a 14year old Maine Coon cat with a perianal abscess, respectively. All three strains could be identified phenotypically, by MALDI-TOF MS analysis and genotypically by sequencing the 16S rDNA and the molecular target genes gap and tuf...
November 7, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29129269/partial-two-thirds-nephrectomy-in-pigs-a-comparison-of-three-surgical-approaches
#2
Hai-Feng Liu, Wei-Jing Meng, Xiang-Dong Kong, Ge Bai, Hui Li, Jian-Tao Zhang, Hong-Gang Fan, Hong-Bin Wang
Partial (2/3) nephrectomy can be performed via the laparoscopic, retroperitoneal, and transperitoneal approach. Outcomes of the three approaches were compared in this study. 2/3 nephrectomy were performed in 21 healthy Bama miniature pigs (mean bodyweight 20.59±2.78kg). Pigs were divided into three groups: those that underwent 2/3 nephrectomy via laparoscopy (LN group, n=7), the retroperitoneal approach (RN group, n=7), or the transperitoneal approach (TN group, n=7). We monitored pre- and postoperative physiologic parameters, blood cell count, and stress and renal function biomarkers...
November 7, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29128114/saturated-or-unsaturated-fat-supplemented-maternal-diets-influence-omental-adipose-tissue-proteome-of-suckling-goat-kids
#3
Laura Restelli, Andreia T Marques, Giovanni Savoini, Guido Invernizzi, Michela Carisetti, Cristina Lecchi, Emoke Bendixen, Fabrizio Ceciliani
The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil...
November 3, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29132684/interactions-between-lactobacilli-and-chicken-macrophages-induce-antiviral-responses-against-avian-influenza-virus
#4
Bahram Shojadoost, Raveendra R Kulkarni, Jennifer T Brisbin, Wanderley Quinteiro-Filho, Tamiru N Alkie, Shayan Sharif
Macrophages are an important cell type of the innate immune system that upon activation, can exert antiviral functions and also can induce virus-specific adaptive immune responses. Macrophage interaction with certain probiotic bacteria such as lactobacilli can enhance antiviral functions of these cells. We have previously shown that administration of lactobacilli to chickens can effectively augment immune response to vaccine antigens. Here, we investigated the effects of representative strains of three Lactobacillus species, L...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29131970/aloe-barbadensis-miller-versus-silver-sulfadiazine-creams-for-wound-healing-by-secondary-intention-in-dogs-and-cats-a-randomized-controlled-study
#5
Dario Drudi, Debora Tinto, Davide Ferranti, Federico Fiorelli, Mara Dal Pozzo, Ombretta Capitani
The objective of this study was to compare the effectiveness of the topical application of Aloe barbadensis Miller (juice and fresh gel) to skin wounds, in dogs and cats, with that of topically applied silver sulfadiazine cream. The sample included 16 patients with cutaneous wounds (13 dogs and three cats) that were divided into three groups. Aloe vera "juice" and "fresh gel" were applied for groups I and II, respectively, while silver sulfadiazine was applied for the control group III. In order to evaluate the healing of wounds, the following parameters were taken into consideration: the percentage of wound shrinkage, the healing time and the macroscopic appearance of the scarring process...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29122298/evoked-potential-and-eeg-study-of-the-neurotoxicity-of-hydramethylnon-in-rats
#6
George M Strain
The objective of the study was to assess the neurotoxicity, using electrodiagnostic tests, of hydramethylnon (Amdro, AC 217,300), an insecticide marketed for the treatment of red imported fire ants, cockroaches, and other insects. Animals were male Fisher 344 albino rats and Long-Evans hooded rats. Brainstem auditory, visual, and somatosensory evoked potentials (BAER, VEP, SEP) and electroencephalograms (EEG) were recorded from implanted screw electrodes before and at multiple time points through day 10 after a single oral dose of hydramethylnon (at 50% or 75% of the LD50) plus vehicle, or vehicle alone...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29113645/discrepancies-between-feline-coronavirus-antibody-and-nucleic-acid-detection-in-effusions-of-cats-with-suspected-feline-infectious-peritonitis
#7
Eleonora Lorusso, Viviana Mari, Michele Losurdo, Gianvito Lanave, Adriana Trotta, Giulia Dowgier, Maria Loredana Colaianni, Andrea Zatelli, Gabriella Elia, Domenico Buonavoglia, Nicola Decaro
Intra-vitam diagnosis of feline infectious peritonitis (FIP) is a challenge for veterinary diagnosticians, since there are no highly specific and sensitive assays currently available. With the aim to contribute to fill this diagnostic gap, a total of 61 effusions from cats with suspected effusive FIP were collected intra-vitam for detection of feline coronavirus (FCoV) antibodies and RNA by means of indirect immunofluorescence (IIF) assay and real-time RT-PCR (qRT-PCR), respectively. In 5 effusions there was no evidence for either FCoV RNA or antibodies, 51 and 52 specimens tested positive by IIF and qRT-PCR, respectively, although antibody titres≥1:1600, which are considered highly suggestive of FIP, were detected only in 37 effusions...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29108655/proteinase-activated-receptor-2-distribution-and-expression-in-equine-small-intestine-tracts-following-herniation-through-the-epiploic-foramen
#8
Noemi Romagnoli, Augusta Zannoni, Chiara Bernardini, Thomas Gobbetti, Cristiano Bombardi, Anna Maria Rambaldi, Maria Morini, Francesco Dondi, Riccardo Rinnovati, Carlotta Lambertini, Alessandro Spadari
Proteinase-activated receptor 2 (PAR2) is a G-protein-coupled receptor for trypsin and mast cell tryptase; it is highly expressed at the intestinal level with multiple functions, such as epithelial permeability and intestinal motility. The aim of the study was to evaluate the distribution and expression of proteinase-activated receptor 2 in the small intestine during herniation through epiploic foramen. In this prospective clinical study, eight horses admitted for colic and which underwent exploratory laparotomy were considered...
October 31, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29113646/effects-of-recombinant-canine-interferon-%C3%AE-injected-before-general-anesthesia-with-propofol-and-isoflurane-on-natural-killer-cytotoxic-activity-during-anesthesia-in-dogs
#9
Hirotaka Matsumoto, Takuma Miyata, Tomoko Ohkusa, Takahiro Teshima, Hidekazu Koyama
We aimed to determine whether subcutaneous injection of recombinant canine interferon-γ (rCaIFN-γ) 1h before general anesthesia with a combination of propofol and isoflurane (P-I) changes the cytotoxic activity of natural killer (NK) cells during anesthesia in dogs. Nine clinically healthy beagles were assigned to one of two groups: rCaIFN-γ-treated group (n=5) and control group (no treatment, n=4). One hour before P-I anesthesia, rCaIFN-γ was subcutaneously injected at a dose of 1×10(4)U/kg body weight for the test group and physiologic saline was subcutaneously injected for the control group...
October 26, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29126629/characterization-of-innate-responses-induced-by-in-ovo-administration-of-encapsulated-and-free-forms-of-ligands-of-toll-like-receptor-4-and-21-in-chicken-embryos
#10
Jegarubee Bavananthasivam, Tamiru N Alkie, Ayumi Matsuyama-Kato, Douglas C Hodgins, Shayan Sharif
Toll-like receptors (TLRs) are a family of innate receptors that recognize pathogen-associated molecular patterns, including double-stranded RNA, CpG DNA and lipopolysaccharide (LPS). After interaction with their ligands, TLRs initiate innate responses that are manifested by activating cells and inducing expression of cytokines that help mediate adaptive immune responses. TLR ligands (TLR-Ls) have the potential to be used prophylactically (alone) or as vaccine adjuvants to promote host immunity. Encapsulating TLR-Ls in nanoparticles, such as Poly (d,l-lactic-co-glycolic acid), may prolong responses through sustained release of the ligands...
October 25, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29126628/effect-of-transportation-on-the-sympatho-adrenal-system-responses-in-horses
#11
Pietro Medica, Giuseppe Bruschetta, Cristina Cravana, Adriana Ferlazzo, Esterina Fazio
The objective of current study was to evaluate the effect of transportation stress on the circulating adrenaline (A), noradrenaline (NA) and dopamine (DA) responses of stallions, according to the different distances. Forty-two stallions were studied before and after road transportation of 100, 200 and 300km, for a period of 1-3h. An increase in plasma A after 100km (P<0.001) was observed. A similar result was seen in plasma NA after 100km (P<0.001), and 300km (P<0.001). Increases in plasma DA after 100 and 200km (P<0...
October 25, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28987957/the-first-report-of-cases-of-pet-dogs-with-naturally-occurring-cancer-treated-with-the-antitumor-peptide-cigb-552
#12
Maribel G Vallespi, Juan C Rodriguez, Lilibet Calaña Seoane, Patricia Alvarez, Hector Santana, Hilda Garay, Ibrahim Acosta Cabrera, Joan Torres Espinosa, Osvaldo Reyes
The absence of an effective therapy against human solid tumors has fostered the development of promising antineoplastic therapeutic candidates, as the CIGB-552 peptide. This synthetic peptide has shown to be effective in reducing tumor size and increasing the lifespan in tumor-bearing mice. Therefore, this work was aimed to explore the safety profile and preliminary assessment of antitumor activity of the CIGB-552 peptide therapeutic candidate in a small population of dogs (n=9) having malignant spontaneously-arising solid tumors...
September 29, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28992489/methotrexate-induces-high-level-of-apoptosis-in-canine-lymphoma-leukemia-cell-lines
#13
Aleksandra Pawlak, Justyna Kutkowska, Bożena Obmińska-Mrukowicz, Andrzej Rapak
INTRODUCTION: Methotrexate is an antimetabolite used in the treatment of cancer and non-malignant diseases including rheumatoid arthritis, psoriasis and graft vs. host disease. Combination therapy with methotrexate was successful in the treatment of canine lymphoma, mammary tumor and invasive urinary bladder cancer. Lymphoma, the most common hematopoietic cancer in dogs, and leukemia are sensitive to chemotherapy, which is why methotrexate may be an important treatment option for these diseases...
September 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28987958/study-of-the-association-of-atmospheric-temperature-and-relative-humidity-with-bulk-tank-milk-somatic-cell-count-in-dairy-herds-using-generalized-additive-mixed-models
#14
Francesco Testa, Giuseppe Marano, Federico Ambrogi, Patrizia Boracchi, Antonio Casula, Elia Biganzoli, Paolo Moroni
Elevated bulk tank milk somatic cell count (BMSCC) has a negative impact on milk production, milk quality, and animal health. Seasonal increases in herd level somatic cell count (SCC) are commonly associated with elevated environmental temperature and humidity. The Temperature Humidity Index (THI) has been developed to measure general environmental stress in dairy cattle; however, additional work is needed to determine a specific effect of the heat stress index on herd-level SCC. Generalized Additive Model methods were used for a flexible exploration of the relationships between daily temperature, relative humidity, and bulk milk somatic cell count...
September 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28985615/body-temperature-and-motion-evaluation-of-an-online-monitoring-system-in-pigs-challenged-with-porcine-reproductive-respiratory-syndrome-virus
#15
Tamás Süli, Máté Halas, Zsófia Benyeda, Réka Boda, Sándor Belák, Marta Martínez-Avilés, Eduardo Fernández-Carrión, José Manuel Sánchez-Vizcaíno
Highly contagious and emerging diseases cause significant losses in the pig producing industry worldwide. Rapid and exact acquisition of real-time data, like body temperature and animal movement from the production facilities would enable early disease detection and facilitate adequate response. In this study, carried out within the European Union research project RAPIDIA FIELD, we tested an online monitoring system on pigs experimentally infected with the East European subtype 3 Porcine Reproductive & Respiratory Syndrome Virus (PRRSV) strain Lena...
September 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29055273/dehydroepiandrosterone-and-cortisol-dehydroepiandrosterone-ratios-in-dairy-cattle-with-postpartum-metritis
#16
Neele Hendrika Gundlach, Maren Feldmann, Yasmin Gundelach, Marcelo Araujo Gil, Ursula Siebert, Martina Hoedemaker, Marion Schmicke
Dehydroepiandrosterone (DHEA), a C19 steroid synthesized predominantly by the adrenal cortex, and its sulfated precursor (DHEAS) has antiglucocorticoid and immunoprotective properties. DHEA is important in modulating inflammatory responses; therefore, we hypothesized that DHEA concentrations might be altered in bovine postpartum metritis. DHEA, DHEAS, the cortisol/DHEA-ratio (C/D-ratio) and the DHEAS/DHEA ratio were determined in cows with and without clinical metritis subcategorized by the blood leukocyte numbers...
September 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28968573/infrared-thermal-imaging-as-a-method-to-evaluate-heat-loss-in-newborn-lambs
#17
L Labeur, G Villiers, A H Small, G N Hinch, S Schmoelzl
Thermal imaging technology has been identified as a potential method for non-invasive study of thermogenesis in the neonatal lamb. In comparison to measurement of the core body temperature, infrared thermography may observe thermal loss and thermogenesis linked to subcutaneous brown fat depots. This study aimed to identify a suitable method to measure heat loss in the neonatal lamb under a cold challenge. During late pregnancy (day 125), ewes were subjected to either shearing (n=15) or mock handling (sham-shorn for 2min mimicking the shearing movements) (n=15)...
September 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28972942/the-effect-of-stable-bedding-materials-on-dust-levels-microbial-air-contamination-and-equine-respiratory-health
#18
Agnieszka Kwiatkowska-Stenzel, Dorota Witkowska, Janina Sowińska, Artur Stopyra
The choice of bedding material affects the quality of air in a stable and, consequently, the respiratory health of horses and humans. The risk of respiratory problems can be mitigated by improving the quality of air in the stable. The choice of bedding material is particularly important in cold climate conditions where horses are kept indoors throughout the year. This study examined the impact of three bedding materials: straw (S), peat with shavings (PS), and crushed wood pellets (CWP). The investigated factors were air contamination, including dust contamination and microbial (bacterial and fungal) contamination, and the condition of the equine respiratory tract...
September 25, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28968572/analyses-of-long-non-coding-rnas-and-mrna-profiling-through-rna-sequencing-of-mdbk-cells-at-different-stages-of-bovine-viral-diarrhea-virus-infection
#19
Qiman Ma, Liangyuan Li, Yan Tang, Qiang Fu, Sheng Liu, Shengwei Hu, Jun Qiao, Chuangfu Chen, Wei Ni
BACKGROUND: Bovine viral diarrhea virus (BVDV) infection is a dynamic and complex process that leads to significant economic losses in the dairy and cattle industries. However, our understanding of the protective and pathological mechanism underlying host infection is limited. METHODS: To determine whether BVDV regulates specific activities of the host cell, the expression of long non-coding RNA (lncRNA) during BVDV NADL infection was studied by deep sequencing...
September 25, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28958409/the-immune-system-is-part-of-a-whole-organism-regulatory-network
#20
EDITORIAL
Gianfranco Gabai, Massimo Amadori, Christopher H Knight, Dirk Werling
No abstract text is available yet for this article.
September 20, 2017: Research in Veterinary Science
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