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Research in Veterinary Science

Kristiane Barington, Henrik E Jensen, Kerstin Skovgaard
Prediction-models based on gene expression profiles from experimental bruises are capable of determining the age of bruises with a precision of ±2 h. However, these models have not yet been applied on tissue from pigs in forensic cases requested by the police. We applied two prediction-models, based on mRNA expression of 13 (prediction-model no. 1) and 4 genes (prediction-model no. 2) involved in inflammation, on forensic cases of porcine bruises in order to determine if gene expression profiles can be used for age determination in forensic cases...
September 3, 2018: Research in Veterinary Science
Chaoying Liu, Chunlan Shan, Qin Dong, Guowen Fu, Ru Zhao, Yulin Yan, Hong Gao
To investigate the effects of pathogenic Escherichia coli high pathogenicity island (HPI) on the expression of inflammatory factors via ubiquitin proteasome pathway. Firstly, the UBC-sus-263 shRNA plasmid was successfully established and transfected into porcine small intestine epithelial cells (IPEC-J2) by liposome to silence the ubiquitinntion gene. Then the IPEC-J2 was infected with E. coli HPI+ and HPI- strains, respectively. Finally, the mRNA of intracellular NF-κB and IκB-α,and the protein levels of NF-κB, IκB-α, TNF-α and IL-1 in IPEC-J2 cell line transfected with UBC-sus-263 shRNA (Ub-shRNA) were detected...
September 3, 2018: Research in Veterinary Science
Vanessa L D Queiroz-Castro, Eduardo P da Costa, Saullo Vinicius P Alves, Abelardo Silva Júnior, Mariana Machado-Neves, José D Guimarães
Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and ovaries from 82 cows that had not been vaccinated against BoHV-1 were collected for serological analysis...
August 31, 2018: Research in Veterinary Science
M A A Soares-Costa, J J N Costa, A W B Silva, E V Cunha, L R F M Paulino, B R Silva, A L C Silva, R van den Hurk, J R V Silva
This study evaluated the effects of frutalin (0.6, 6.0 or 60.0 μg/mL) and doxorubicin (0.3 μg/mL) on survival, growth and ultrastructure of in-vitro cultured goat secondary follicles. The effects of these substances on the levels of mRNA for Casp3, Casp6, Bax, and Bcl2 were also investigated. Results showed that, after 6 days of culture, frutalin or doxorubicin reduced the percentage of normal follicles (P < 0.05), but doxorubicin had higher toxicity than frutalin. Except for follicles cultured with 0...
August 30, 2018: Research in Veterinary Science
Ratchaneewan Khiaosa-Ard, Qendrim Zebeli
Dietary shifts play an important role in decreased longevity in ruminant livestock. Ruminants evolved as cellulose fermenters adapt to fiber-rich diets. Instead, high-producing ruminants nowadays are commonly fed with grain-based diets to increase intake and productivity. Such diets, however, trade off the health of the animal. One negative aspect of such feeding is related to elevated levels of bacterial endotoxin (lipopolysaccharide, LPS) in the gut lumen and the likelihood of LPS translocation across the gut causing systemic and local (tissue) inflammation with consequences for production and longevity...
August 24, 2018: Research in Veterinary Science
Dagmara Miłek, Aleksandra Woźniak, Tomasz Stadejek
In the last years several novel parvoviruses (PPVs) were discovered in pigs worldwide. The most recently discovered porcine parvovirus species is PPV7, which was detected in USA and China to date. This study reports the first evidence of PPV7 in Europe. Overall, 902 serum samples and 896 fecal samples were collected between 2014 and 2017 from 3 to 20 weeks old pigs from 14 conventional swine farms in Poland. PPV7 DNA was detected in samples from all examined farms. Overall, PPV7 was detected in 39,0% fecal pools and in 19,6% serum pools...
August 23, 2018: Research in Veterinary Science
C X Wang, S Niu, A R Jahejo, F J Jia, Z Li, N Zhang, G B Ning, D Zhang, H Q Li, H L Ma, W F Hao, W W Gao, S M Gao, J H Li, G L Li, F Yan, R K Gao, Y J Zhao, H C Chen, W X Tian
Thiram, a carbamate pesticide, is known to induce tibial dyschondroplasia (TD) in broiler chickens. This study used a thiram-induced TD model to explore whether apoptosis-related genes were expressed in erythrocytes of broiler chickens and the impacts of thiram-induced TD and the recombinant GSTA3 protein in regulating these genes expression. In this study, mRNA and protein expression of six types of apoptosis-related genes (Bcl-2, Bax, Murine double minute MDM2, Bcl-2-associated athanogene BAG-1, BAG-3, STAT3) were identified in erythrocytes of broiler chickens by real-time PCR and immunohistochemistry, and we also found that thiram-induced TD induced the decreased expression of these antiapoptotic genes in the initial stage of TD and promoted their expression in TD recovery, which suggested that the expression of these apoptosis-related genes in erythrocytes is highly related to the development of TD...
August 23, 2018: Research in Veterinary Science
Jianzhong Wang, Yanlong Jiang, Wentao Yang, Chunwei Shi, Haibin Huang, Hao Sun, Gaosheng Liu, Chunfeng Wang, Guilian Yang, Yanan Cai
Trichinella spiralis is an intestinal nematode parasite that can cause trichinellosis in humans and animals worldwide. The most important known hosts of T. spiralis are pigs, horses, dogs and cats. Pork and its products are the main sources of infection in human trichinellosis. Vaccines against these infections are urgently needed. In this study, the genes encoding the 43-kDa or 45-kDa glycoprotein present in the excretory-secretory (ES) products from T. spiralis muscle larvae (ML) were cloned into the eukaryotic expression vector pVAX1, resulting plasmids pVAX1-Ts43 and pVAX1-Ts45, respectively...
August 23, 2018: Research in Veterinary Science
Gianfranco Gabai, Enrico Novelli
No abstract text is available yet for this article.
August 22, 2018: Research in Veterinary Science
Adam Tarko, Aneta Štochmalova, Sandra Hrabovszka, Adriana Vachanova, Abdel Halim Harrath, Saleh Alwasel, Roland Grossman, Alexander V Sirotkin
Exposure to xylene leads to dysfunction of mammalian female reproduction. Quercetin present in vegetables contribute significantly to their role as health-promoting foods. The effects of xylene and quercetin on ovarian cell function, their interrelationships, and mechanisms of action are insufficiently studied. In this in vitro study, we examined the effects of xylene, quercetin, and xylene/quercetin combination on basic bovine ovarian cell functions, such as proliferation, apoptosis, and hormone release. Furthermore, we examined the protective effect of quercetin against the potential negative effects of xylene...
August 2018: Research in Veterinary Science
E Semik-Gurgul, T Ząbek, A Fornal, M Wnuk, K Pawlina-Tyszko, A Gurgul, J Klukowska-Rötzler, C Koch, K Mählmann, M Bugno-Poniewierska
Genetic and epigenetic alterations in the equine sarcoid, a locally invasive skin tumour of equids, are still poorly characterized. Numerous studies have provided reliable evidence for the relationship between the development of cancer and the loss of function of a number of tumour suppressor genes. In the present study, we assessed methylation levels in the promoter region of SFN, S100A14 and POU2F3 genes in sarcoid samples to clarify whether DNA methylation may be associated with previously identified changes in the expression level of these genes during the course of tumour progression...
August 2018: Research in Veterinary Science
Álvaro Álvarez-Estrada, Sonia Martínez-Martínez, César-Bernardo Gutiérrez Martín, María-José García-Iglesias, Claudia Pérez-Martínez, Sheila Yubero-Delgado, João Antônio Guizzo, Rafael Frandoloso, Elías-Fernando Rodríguez-Ferri
Three recombinant outer membrane proteins (rOmps) from the Haemophilus parasuis Nagasaki strain (serovar 5 reference strain), rOmpP2, rOmpP5 and rOmpD15, which have previously shown protection against H. parasuis infection in mice, were cloned, expressed and evaluated as vaccine antigens in colostrum-deprived pigs. When these animals were immunized with these rOmps and were later challenged intratracheally with 108 CFUs of the Nagasaki strain, no protection was seen in terms of survival, clinical signs, pathological results and recovery of H...
August 2018: Research in Veterinary Science
Qianzhen Zhang, Yansong Ge, Hui Li, Ge Bai, Zhihui Jiao, Xiangdong Kong, Weijing Meng, Hongbin Wang
Hepatic ischemia reperfusion injury (HIRI) occurs commonly in liver surgery and liver transplantation. Hydrogen, a safe and effective antioxidant, exerts a protective effect against liver injury. In this study, we investigated the role of hydrogen-rich saline (HRS) in apoptosis in a miniature pig model of laparoscopic HIRI upon hepatectomy. Bama miniature pigs were randomly assigned to sham, I/R and HRS groups. The pigs received 10 mL/kg HRS by portal venous injection 10 min before reperfusion and at 1 d, 2 d, and 3 d after surgery...
August 2018: Research in Veterinary Science
Benjamin Andreas Berk, Rowena Mary Anne Packer, Tsz Hong Law, Holger Andreas Volk
Epilepsy is the most common chronic neurological disorder in dogs. Some diets have been shown to have a positive impact upon the seizure activity in dogs with idiopathic epilepsy (IE), while other diets and dietary supplements (DS), although marketed as providing health benefits, lack conclusive scientific evidence on their actual beneficial effects. A web-based owner questionnaire was designed to assess how and why owners of dogs with IE use different dietary regimes and DS. The study cohort, with 297 valid responses, consisted mainly of pure-breed (82...
August 2018: Research in Veterinary Science
Seval Yilmaz, Emre Kaya, Aysegul Karaca, Ozhan Karatas
This study was conducted to investigate the protective effects of lycopene against the toxic effects of Aflatoxin B1 (AFB1 ) exposure in kidney and heart of rat by evaluating antioxidant defense systems and lipid peroxidation (LPO). Forty-two healthy three-month-old male Wistar-Albino rats were used in this study. The animals were randomly divided into six experimental groups including 7 rats in each. These groups were arranged as follows: control group, lycopene (5 mg/kg/day, orally for 15 days) group, AFB1 (0...
August 2018: Research in Veterinary Science
Qiu Jue Wu, Xiao Chuan Zheng, Tian Wang, Tie Ying Zhang
The aim of this study was to investigate the effects of oridonin (ORI) on the immune cells, Th1 /Th2 balance and the expression of B lymphocyte stimulator (BLys) in the spleens of broilers infected with Salmonella pullorum. In a completely randomized design, 300 one-day-old AA male broilers were divided to 5 treatments. The groups included a noninfection control (CON) group receiveed a basal diet; a S. pullorum infect control group received the basal diet; and S. pullorum infect group receiveed the basal diet plus 50, 80, and 100 mg/kg ORI, respectively...
August 2018: Research in Veterinary Science
Bo Hu, Zhiyu Fan, Houjun Wei, Yanhua Song, Rulong Qiu, Mengmeng Chen, Weizhong Xu, Jiabin Xue, Fang Wang
During the past years epizootic outbreaks of mucoid enteropathy syndrome (MES) disease have emerged and spread rapidly in rabbit farms in East China causing great economic losses. To investigate the nature and evolution of the disease, two trials were conducted in an attempt to reproduce the disease and to assess the microbiota cecal profile changes associated with the disease. In the first trial, twenty 6-week-old New Zealand White healthy rabbits were assigned randomly to one of two treatments: 1) Inoculation with 2...
August 2018: Research in Veterinary Science
J H J Bittar, A Hoyos-Jaramillo, D J Hurley, A R Woolums, L J Havenga, J M Lourenço, G Barnett, V Gomes, J T Saliki, D D Harmon, R A Palomares
The objective was to evaluate the effects of injectable trace minerals (ITM) concurrent with modified-live virus (MLV) vaccination on protection from bovine viral diarrhea virus (BVDV) infection in dairy calves. In a previous study (Palomares et al., 2016), thirty dairy calves received two doses of a MLV vaccine subcutaneously (SC), concurrently with ITM (n = 15) or saline (n = 15), SC. Five months later, 20 of these calves received ITM (G1, n = 10) or saline (G2, n = 10) according to their previous groups and were challenged intranasally with BVDV2...
August 2018: Research in Veterinary Science
L B Jensen, T Birk, B Borck Høg, L Stehr, S Aabo, H Korsgaard
Cross and co-resistance to antimicrobials are presented for 765 Danish Escherichia coli isolates of porcine origin from 2009 to 2013. All isolates and data originate from the DANMAP surveillance but have not previously been used to describe the occurrence of cross and co- resistance. Data presented here clearly indicate the ability of low classified antimicrobials as ampicillin to uphold resistance to critical important antimicrobials for human treatment.
August 2018: Research in Veterinary Science
Michele Losurdo, Giulia Dowgier, Maria Stella Lucente, Mario Cinone, Michele Camero, Maria Loredana Colaianni, Giuseppe Passantino, Gianluca Ventriglia, Domenico Buonavoglia, Nicola Decaro
The long-term shedding of Canine alphaherpesvirus 1 (CaHV-1) by neonatal pups with natural infection is reported. The pups belonged to a litter of 11 pointers of a breeding kennel in southern Italy, 9 of which developed a fatal form of systemic infection, as resulted by the detection of CaHV-1 in internal organs (kidney, liver, lung and brain) of one of this dogs and in the vaginal swab of their mother. The two remaining animals displayed a milder form of disease, with one pup showing ocular involvement, and underwent a progressive recovery...
August 2018: Research in Veterinary Science
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