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Research in Veterinary Science

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https://www.readbyqxmd.com/read/28735241/a-new-integrated-approach-to-analyze-bulk-tank-milk-and-raw-milk-filters-for-the-presence-of-the-e-coli-serogroups-frequently-associated-with-vtec-status
#1
Francesca Albonico, Claudia Gusmara, Theo Gugliotta, Monica Loiacono, Michele Mortarino, Alfonso Zecconi
We optimized a combination of microbiological and molecular methods to quickly identify the presence of the O157 and the six non-O157 serogroups (O26, O45, O103, O111, O121 and O145) most frequently associated with VTEC status, at herd level. The lower detection limit of this methodology is 10(1)CFU/ml for each of the serogroups tested. We tested 67 bulk tank milk (BTM) and raw milk filters (RMF) derived from dairy herds located in Lombardy and Trentino Alto Adige. We identified 3 positive samples and 20 positive samples out of 67 respectively in the BTM and RMF...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28735242/transcriptomic-analysis-of-the-larva-taenia-multiceps
#2
W H Li, N Z Zhang, L Yue, Y Yang, L Li, H B Yan, T T Li, Z G Qu, W Z Jia, B Q Fu
Taenia multiceps is an adult worm affiliated to Taeniidae family, Platyhelminthes phylum. The larvae of the parasite (Coenurus cerebralis) parasitic in the brain and spinal cord in domestic and wild ruminants or humans can led to a fatal central nervous system (CNS) disease. The aims of the present study were to define the transcriptome profiles of the larvae of T. multiceps by RNA-Seq approach, and to generate large functional gene datasets that could be used to predict the key molecular pathways linked to this cestode...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28715672/genomic-and-pathogenic-analysis-of-a-muscovy-duck-parvovirus-strain-causing-short-beak-and-dwarfism-syndrome-without-tongue-protrusion
#3
Qiuling Fu, Yu Huang, Chunhe Wan, Guanghua Fu, Baomin Qi, Longfei Cheng, Shaohua Shi, Hongmei Chen, Rongchang Liu, Zhenhai Chen
In 2008, clinical cases of short beak and dwarfism syndrome (SBDS) caused by Muscovy duck parvovirus (MDPV) infection were found in mule duck and Taiwan white duck farms in Fujian, China. A MDPV LH strain causing duck SBDS without tongue protrusion was isolated in this study. Phylogenetic analysis show that the MDPV LH strain was clustered together with other MDPV strains, but divergent from GPV isolates. Two major fragment deletions were found in the inverted terminal repeats (ITR) of MDPV LH similar to the ones in the ITR of MDPV GX5, YY and SAAS-SHNH strains...
July 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28711697/role-of-muscarinic-receptors-in-the-contraction-of-jejunal-smooth-muscle-in-the-horse-an-in-vitro-study
#4
Alessandro Menozzi, Cristina Pozzoli, Enzo Poli, Giada Bontempi, Paolo Serventi, Valentina Meucci, Luigi Intorre, Simone Bertini
Nonselective antimuscarinic drugs are clinically useful in several pathologic conditions of horses, but, blocking all muscarinic receptor (MR) subtypes, may cause several side effects. The availability of selective antimuscarinic drugs could improve therapeutic efficacy and safety. We aimed to enlighten the role of different MR subtypes by evaluating the effects of nonselective, and selective M1, M2 and M3 MR antagonists on the contractions of horse jejunum. Segments of circular muscle of equine jejunum, were put into organ baths, connected to isotonic transducers, and the effects on ACh concentration-response curves, and on electrical field stimulation (EFS)-evoked contractions of intestinal preparations, induced by nonselective or selective MR antagonists, compared to pre-drug level, were studied...
July 11, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28711819/differentiation-potential-of-synoviocytes-derived-from-joints-with-cranial-cruciate-ligament-rupture-and-medial-patella-luxation-in-dogs
#5
H M Suranji Wijekoon, Kazuhide Toyota, Sangho Kim, Jing Fang, Eugene C Bwalya, Kenji Hosoya, Masahiro Okumura
The objective of this study was to assess the differentiation capability of synoviocytes derived from dogs with inflammatory joint conditions. Cranial cruciate ligament ruptured (CCLr) (n=12) and medial patella luxated (MPL) (n=10) knee joints of the dogs were used to collect the synovial membrane (SM). Synoviocytes were enzymatically released from the SM and analyzed by flow cytometry for specific cellular markers (CD44 and CD90) of mesenchymal stem cells (MSCs), while doing histopathology from another part of SM sections...
July 9, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28711694/normal-ultrasonographic-reference-values-for-the-gastrointestinal-tract-in-developing-puppies
#6
Tommaso Banzato, Chiara Milani, Elena Zambello, Alessandro Zotti
Thickness of the individual layers of the wall of the stomach, duodenum, jejunum and colon was assessed by means of ultrasonography in developing puppies at 4, 8 and 16weeks of age. Reference intervals for the thickness of individual ultrasonographic layers of the stomach, duodenum, jejunum and colon at different ages are reported. An increase in wall thickness of all the examined gastro-intestinal tracts in relation to age was recorded. The effect of body-weight was stronger on duodenal and jejunal thickness whereas it resulted lesser on stomach and colon...
July 9, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28710961/epigenetic-modification-differences-between-fetal-fibroblast-cells-and-mesenchymal-stem-cells-of-the-arbas-cashmere-goat
#7
Xiao Wang, Zhimin Wang, Qing Wang, Hefei Wang, Hao Liang, Dongjun Liu
To explore the epigenetic mechanisms regulating mesenchymal stem cells, we analyzed epigenetic patterns in control goat fetal fibroblast cells (gFFCs), adipose-derived stem cells (gADSCs), bone marrow stromal cells (gBMSCs), and muscle-derived satellite cells (gMDSCs). We found that the 5mC content of gBMSC genomes was lower than that of gFFC genomes, while the 5mC content of gADSC and gMDSC genomes surpassed that of gFFC genomes. H3K9 acetylation did not differ significantly among those cells; gFFCs, gADSCs, and gMDSCs contained acetylated H3K9, H3K14, H3K18, H4K5, and H4K12, but gBMSCs contained almost no acetylated H4K5 and H4K12...
July 9, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28710955/epidemiologic-evaluation-of-canine-urolithiasis-in-thailand-from-2009-to-2015
#8
Vachira Hunprasit, Carl A Osborne, Pamela J Schreiner, Jeff B Bender, Jody P Lulich
The cross-sectional study described the epidemiology of 8560 canine urolith submissions from Thailand to the Minnesota Urolith Center between January 2009 and December 2015. The frequency of urolith types, the relationships between urolith type and breed, sex, and neutered status, and change of annual submission proportion over the study period were analyzed. Struvite was the most common canine urolith (44%), and was commonly found in intact females with a mean age of 6.3±3.1years. Calcium oxalate was the second most common (27%), more frequently found in intact males with a mean age of 8...
July 9, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28738285/molecular-assessment-characterization-and-differentiation-of-theca-stem-cells-imply-the-presence-of-mesenchymal-and-pluripotent-stem-cells-in-sheep-ovarian-theca-layer
#9
Samane Adib, Mojtaba Rezazadeh Valojerdi
The ability of ovarian theca stem cells to differentiate into oocyte and theca cells may lead to a major advancement in reproductive biology and infertility treatments. However, there is little information about function, growth and differentiation potential of these immature cells. In this study adult sheep theca stem cells (TSCs) characteristics, and differentiation potential into osteocyte-like cells (OSLCs), adipocyte-like cells (ALCs), theca progenitor-like cells (TPCs), and oocyte-like cells (OLCs) were investigated...
July 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28711696/week-to-week-variation-of-scintigraphic-semi-quantitative-thyroid-variables-in-14-healthy-experimental-cats
#10
Veerle Volckaert, Eva Vandermeulen, Emmelie Stock, Luc Duchateau, Sylvie Daminet, Myriam Hesta, Jimmy Saunders, Robrecht Dockx, Kathelijne Peremans
Although scintigraphy is an important tool for the assessment of thyroid function in cats, time variation of commonly used thyroid variables has not been investigated to this day. The aim of this study was to investigate whether a week-to-week variation of scintigraphic variables exists in healthy cats of a magnitude that is clinically relevant, as this could lead to misinterpretation of results. Fourteen adult, healthy, experimental cats were included in the study. At 3 time points, with 7-day intervals, the cats underwent a thyroid pertechnetate scan and blood samples were collected...
July 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28711695/identification-of-a-conformational-neutralizing-epitope-on-the-vp1-protein-of-type-a-foot-and-mouth-disease-virus
#11
Wenming Liu, Baolin Yang, Mingxia Wang, Haiwei Wang, Decheng Yang, Wenge Ma, Guohui Zhou, Li Yu
Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. In recent years, outbreaks of serotype A FMD have occurred in many countries. High-affinity neutralizing antibodies against a conserved epitope could provide protective immunity against diverse subtypes of FMDV serotype A and protect against future pandemics. In this study, we generated a serotype A FMDV-specific potent neutralizing monoclonal antibody (MAb), 6C9, which recognizes a conformation-dependent epitope...
July 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28693904/corrigendum-to-dietary-allium-hookeri-reduces-inflammatory-response-and-increases-expression-of-intestinal-tight-junction-proteins-in-lps-induced-young-broiler-chicken-res-vet-sci-112-149-155
#12
Youngsub Lee, Sung-Hyen Lee, Ujvala Deepthi Gadde, Sung-Taek Oh, Sung-Jin Lee, Hyun S Lillehoj
No abstract text is available yet for this article.
July 7, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28709108/vibrio-parahaemolyticus-and-v-alginolyticus-associated-meningo-encephalitis-in-a-bottlenose-dolphin-tursiops-truncatus-from-the-adriatic-coast-of-italy
#13
Ludovica Di Renzo, Gabriella Di Francesco, Chiara Profico, Cristina E Di Francesco, Nicola Ferri, Daniela Averaimo, Giovanni Di Guardo
A case of Vibrio parahaemolyticus- and V. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (Tursiops truncatus) found stranded along the Adriatic coast of Italy in 2016 is herein reported, along with a minireview on V. parahaemolyticus and V. alginolyticus infections in aquatic mammals. Macroscopically, two abscesses were found in the dolphin's forebrain, along with an extensive, bilateral, parasitic broncho-pneumonia. Histologically, a suppurative-to-pyogranulomatous meningo-encephalitis involved the brain but not the cerebellum...
July 1, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28689144/sequence-variation-in-the-b1-gene-among-toxoplasma-gondii-isolates-from-swine-and-cats-in-italy
#14
Azzurra Santoro, Fabrizia Veronesi, Giovanni Luigi Milardi, David Ranucci, Raffaella Branciari, Manuela Diaferia, Simona Gabrielli
The evaluation of the genetic variations of Toxoplasma gondii among isolates of a wide variety of animal hosts can provide significant information for better understanding the epidemiology and population structure of the parasite in different geographical areas. The aim of this study was to provide information on T. gondii genetic diversity in host species living in central Italy, which could act as a potential source of human infection. Seventy-seven feline faecal samples, and 36 and 20 diaphragm pillar tissue samples from pigs and wild boars were collected in Umbria (central Italy)...
July 1, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28692924/experimental-co-infection-of-infectious-bronchitis-and-low-pathogenic-avian-influenza-h9n2-viruses-in-commercial-broiler-chickens
#15
Kareem E Hassan, Ahmed Ali, Salama A S Shany, Magdy F El-Kady
In this study, commercial broilers were experimentally infected with single (classical IBV, variant IBV or AIV-H9N2) or mixed AIV-H9N2 with classical, variant or vaccine strains of IBV. Birds were monitored for clinical and pathological outcomes and virus shedding for 10days post infection (DPI). Clinical signs were limited to the respiratory tract in all challenged groups and varied from mild to moderate mouth breathing to severe respiratory signs with snorting sound and extended head. Mortalities were only recorded in mixed AIV-H9N2/variant IBV challenge group...
June 30, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28688366/differentiation-of-flavobacterium-psychrophilum-from-flavobacterium-psychrophilum-like-species-by-maldi-tof-mass-spectrometry
#16
Marta Pérez-Sancho, Ana Isabel Vela, Tom Wiklund, Markus Kostrzewa, Lucas Domínguez, José Francisco Fernández-Garayzábal
Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific identification of F...
June 29, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28672254/orally-administered-recombinant-lactobacillus-casei-vector-vaccine-expressing-%C3%AE-toxoid-of-clostridium-perfringens-that-induced-protective-immunity-responses
#17
Mojtaba Alimolaei, Mehdi Golchin, Majid Ezatkhah
Clostridium perfringens types B and C cause enteritis and enterotoxemia in animals. The conventional vaccine production systems need time-consuming detoxification and difficult quality control steps. In this study, a modified β-toxoid gene was synthesized, cloned into the pT1NX vector, and electroporated into Lactobacillus casei competent cells to yield L. casei-β recombinant strain. Surface expression of the recombinant β-toxoid was evaluated by ELISA and confirmed by immunofluorescence microscopy. Vaccinated BALB/c mice with L...
June 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28688615/innovative-dairy-cow-management-to-improve-resistance-to-metabolic-and-infectious-diseases-during-the-transition-period
#18
REVIEW
P Lacasse, N Vanacker, S Ollier, C Ster
The incidence of metabolic and infectious diseases varies greatly during the lactation cycle. Most new cases of clinical mastitis appear at the beginning of lactation, and the incidence increases with the level of milk production. In addition to mastitis, many other infectious diseases become clinically apparent during the first 2weeks of lactation. During this time, cows are in a negative energy balance and must mobilize body reserves to balance the deficit between food energy intake and energy required for milk production...
June 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28672253/accuracy-of-a-cow-side-test-for-the-diagnosis-of-hyperketonemia-and-hypoglycemia-in-lactating-dairy-cows
#19
K Macmillan, I López Helguera, A Behrouzi, M Gobikrushanth, B Hoff, M G Colazo
The objective of this study was to evaluate the use of a cow-side device (FreeStyle Precision Neo™) to diagnose ketosis and hypoglycemia based on measures of blood β-hydroxybutyrate (BHBA) and glucose. Eleven commercial dairy farms were visited and blood samples were taken from Holstein cows between 2 and 14days in milk, yielding 441 samples for BHBA analysis and 308 samples for glucose analysis. Concentrations of BHBA and glucose were measured in two ways, 1) using the cow-side device with whole blood immediately after sampling and 2) serum samples analyzed with a standard laboratory assay (Animal Health Laboratory, University of Guelph, Canada)...
June 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28668734/can-digital-dermatitis-be-detected-in-the-milking-parlor-without-washing-cows-feet
#20
Victor H S Oliveira, Jan T Sørensen, Peter T Thomsen
Scoring digital dermatitis (DD) in the milking parlor after washing the hind feet of cows has been recommended. However, farmers might be reluctant to perform this washing due to compromised udder hygiene. The objective of this study was to evaluate if DD prevalence can be determined without the washing procedure. A total of 4510 cows from 22 dairy herds were scored for DD in the milking parlor without and with washing their hind feet. In all DD infected herds, DD prevalence was higher when scored with washing...
June 27, 2017: Research in Veterinary Science
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