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Plant Physiology

Yuan Zheng, Zhaojin Chen, Liang Ma, Chancan Liao
The phytohormone abscisic acid (ABA) is critical for plants encountering abiotic stress. We previously reported that the Arabidopsis (Arabidopsis thaliana) transcription factor MYB30 participates in ABA responses via SUMO ligase SAP-MIZ Domain-Containing SIZ1-mediated sumoylation. Here, we show that the RING-type ubiquitin E3 ligase RHA2b, which positively regulates ABA signaling, interacted with and ubiquitinated MYB30 to modulate MYB30 stability through the 26S proteasome pathway. The degradation rate of MYB30 was significantly repressed in the rha2b-1 mutant...
July 20, 2018: Plant Physiology
Kevin Magne, Jean-Malo Couzigou, Katharina Schiessl, Shengbin Liu, Jeoffrey George, Vladimir Zhukov, Lucien Sahl, Frederic Boyer, Anelia Iantcheva, Kirankumar S Mysore, Jiangqi Wen, Sylvie Citerne, Giles E Oldroyd, Pascal Ratet
Symbiotic interactions between legume plants and rhizobia result in the formation of nitrogen-fixing nodules, but the molecular actors and the mechanisms allowing for the maintenance of nodule identity are poorly understood. Medicago truncatula NODULE ROOT1 (MtNOOT1), Pisum sativum COCHLEATA1 (PsCOCH1) and Lotus japonicus NOOT-BOP-COCH-LIKE1 (LjNBCL1) are orthologs of Arabidopsis (Arabidopsis thaliana) AtBLADE-ON-PETIOLE1/2 and are members of the NOOT-BOP-COCH-LIKE (NBCL) gene family, which has conserved roles in plant development and is essential for indeterminate and determinate nodule identity in legumes...
July 19, 2018: Plant Physiology
Ke-Lin Huang, Guang-Jing Ma, Mei-Li Zhang, Huan Xiong, Huan Wu, Cai-Zhi Zhao, Chun-Sen Liu, Han-Xin Jia, Liang Chen, Johan Olav Kjorven, Xue-Bao Li, Feng Ren
PHOSPHATE STARVATION RESPONSE 1 (PHR1) is a key regulatory component of the response to phosphate (Pi) starvation. However, regulation of PHR1 in this response remains poorly understood. Here, we report that PHR1 is a target of the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 and is positively regulated by auxin signaling in Arabidopsis thaliana roots. PHR1 expression was induced by exogenous auxin and suppressed by auxin transport inhibitors in Arabidopsis roots. In the PHR1 promoter, three auxin-response elements, which are directly bound by ARF7 and ARF19, were shown to be essential for PHR1 expression...
July 19, 2018: Plant Physiology
Ann-Kathrin Schürholz, Vadir Lopez-Salmeron, Zhenni Li, Joachim Forner, Christian Wenzl, Christophe Gaillochet, Sebastian Augustin, Amaya Vilches-Barro, Michael Fuchs, Michael Gebert, Jan U Lohmann, Thomas Greb, Sebastian Wolf
Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell type-specific trans-activation in Arabidopsis thaliana (Arabidopsis) based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate cloning system, we produced a comprehensive set of transgenic lines termed GR-LhG4 driver lines targeting most tissues in the Arabidopsis shoot and root with a strong focus on the indeterminate meristems...
July 19, 2018: Plant Physiology
Dandan Qian, Guoqiang Chen, Lihong Tian, Le Qing Qu
Endoplasmic reticulum-associated protein degradation (ERAD) plays an important role in endoplasmic reticulum (ER) quality control. To date, little is known about the retrotranslocation machinery in the plant ERAD pathway. We obtained a DERLIN-like protein (OsDER1) through a SWATH-based quantitative proteomic analysis of ER membrane proteins extracted from ER-stressed rice (Oryza sativa) seeds. OsDER1, a homolog of yeast and mammal DER1, is localized in the ER and accumulates significantly under ER stress. Overexpression or suppression of OsDER1 in rice leads to activation of the unfolded protein response and hypersensitivity to ER stress, and suppression results in floury, shrunken seeds...
July 19, 2018: Plant Physiology
Hugo Alonso-Cantabrana, Asaph B Cousins, Florence R Danila, Timothy Ryan, Robert E Sharwood, Susanne von Caemmerer, Robert T Furbank
Phosphoenolpyruvate carboxylase (PEPC), localised to the cytosol of mesophyll cell, catalyzes the first carboxylation step of the C4 photosynthetic pathway. Here, we used RNA interference to target the cytosolic photosynthetic PEPC isoform in Setaria viridis and isolated independent transformants with very low PEPC activities. These plants required high ambient CO2 concentrations for growth, consistent with the essential role of PEPC in the C4 photosynthesis. The combination of estimating direct CO2 fixation by the bundle sheath using gas exchange measurements and modelling C4 photosynthesis with low PEPC activity allowed the calculation of bundle sheath conductance to CO2 diffusion (gbs) in the progeny of these plants...
July 17, 2018: Plant Physiology
Ke Wang, Zong-Li Guo, Wen-Tao Zhou, Cheng Zhang, Ze-Yuan Zhang, Yue Lou, Shuang-Xi Xiong, Xiaozhen Yao, Jiong-Jiong Fan, Jun Zhu, Zhong-Nan Yang
The outer pollen wall (sexine) protects pollen and contains sporopollenin, which is synthesized using a pathway of approximately eight genes in Arabidopsis thaliana. MALE STERILITY 188 (MS188) and its direct upstream regulator ABORTED MICROSPORES (AMS) are two transcription factors essential for tapetum development. Here, we show that all the sporopollenin biosynthesis proteins are specifically expressed in the tapetum and are secreted into anther locules. MS188, a MYB transcription factor expressed in the tapetum, directly regulates the expression of POLYKETIDE SYNTHASE A (PKSA), PKSB, MALE STERILE2 (MS2), and a CYTOCHROME P450 gene (CYP703A2)...
July 17, 2018: Plant Physiology
Jing Wang, Jawon Song, G B Clark, Stanley J Roux
Annexins are a multigene family of calcium-dependent membrane-binding proteins that play important roles in plant cell signalling. Annexins are multifunctional proteins and their function in plants is not comprehensively understood. Arabidopsis thaliana annexins AnnAt1 and AnnAt2 are 64% identical in their primary structure, and both are highly expressed in seedlings. Here, we showed that annAt-mutant seedlings grown in the absence of sugar show decreased primary root growth and altered columella cells in root caps; however, these mutant defects are rescued by sucrose, glucose or fructose...
July 17, 2018: Plant Physiology
Kyle A Pelot, Ruibing Chen, David M Hagelthorn, Cari A Young, J Bennett Addison, Andrew Muchlinski, Dorothea Tholl, Philipp Zerbe
Diterpenoids constitute a diverse class of metabolites with critical functions in plant development, defense, and ecological adaptation. Major monocot crops, such as maize (Zea mays) and rice (Oryza sativa), deploy diverse blends of specialized diterpenoids as core components of biotic and abiotic stress resilience. Here, we describe the genome-wide identification and functional characterization of stress-related diterpene synthases (diTPSs) in the dedicated bioenergy crop switchgrass (Panicum virgatum). Mining of the allotetraploid switchgrass genome identified an expansive diTPS family of 31 members, and biochemical analysis of 11 diTPSs revealed a modular metabolic network producing a diverse array of diterpenoid metabolites...
July 15, 2018: Plant Physiology
Yuling Jiao
No abstract text is available yet for this article.
July 15, 2018: Plant Physiology
Said S Hafidh, David Potěšil, Karel Müller, Jan Fíla, Christos Michailidis, Anna Herrmannová, Jana Feciková, Till Ischebeck, Leoš Shivaya Valášek, Zbyněk Zdráhal, David Honys
Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are later utilized for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum) - long-term storage EDTA/puromycine-resistant particles (EPPs), translating polysomes, and free ribonuclear particles - throughout tobacco pollen development and in in vitro growing pollen tubes...
July 14, 2018: Plant Physiology
Wei Shen, Benjamin G Bobay, Laura A Greeley, Maria I Reyes, Cyprian A Rajabu, Robert Kevin Blackburn, Mary Beth Dallas, Michael B Goshe, Jose Trinidad Ascencio-Ibanez, Linda Hanley-Bowdoin
Geminiviruses are single-stranded DNA viruses that infect a wide variety of plants and cause severe crop losses worldwide. The geminivirus replication initiator protein (Rep) binds to the viral replication origin and catalyzes DNA cleavage and ligation to initiate rolling circle replication. In this study, we found that the Tomato golden mosaic virus (TGMV) Rep is phosphorylated at Ser-97 by sucrose non-fermenting 1 (SNF1)-related protein kinase 1 (SnRK1), a master regulator of plant energy homeostasis and metabolism...
July 13, 2018: Plant Physiology
Yan Zubo, Ivory C Blakley, Jose M Franco-Zorrilla, Maria V Yamburenko, Roberto Solano, Joseph J Kieber, Ann E Loraine, G Eric Schaller
Fundamental questions regarding how chloroplasts develop from proplastids remain poorly understood despite their central importance to plant life. Two families of nuclear transcription factors, the GATA NITRATE-INDUCIBLE CARBON-METABOLISM-INVOLVED (GNC) and GOLDEN TWO-LIKE (GLK) families, have been implicated in directly and positively regulating chloroplast development. Here, we determined the degree of functional overlap between the two transcription factor families in Arabidopsis thaliana, characterizing their ability to regulate chloroplast biogenesis both alone and in concert...
July 12, 2018: Plant Physiology
Farzaneh Tamnanloo, Hassan Damen, Raman Jangra, Jin Suk Lee
Stomata on the plant epidermis control gas and water exchange and are formed by mitogen-activated protein kinase (MAPK)-dependent processes. Although the contribution of MAP KINASE3 (MPK3) and MPK6 (MPK3/MPK6) to the control of stomatal patterning and differentiation in Arabidopsis (Arabidopsis thaliana) has been examined extensively, how they are inactivated and regulate distinct stages of stomatal development is unknown. Here, we identify a dual-specificity phosphatase, MAP KINASE PHOSPHATASE1 (MKP1), which promotes stomatal cell fate transition by controlling MAPK activation at the early stage of stomatal development...
July 12, 2018: Plant Physiology
Yuanyuan Zhang, Kai Chen, Fangjie Zhao, Cuiju Sun, Cheng Jin, Yuheng Shi, Yangyang Sun, Yuan Li, Meng Yang, Xinyu Jing, Jie Luo, Xingming Lian
Copper (Cu) is an essential micronutrient for plant growth. However, the molecular mechanisms underlying Cu trafficking and distribution to different organs in rice are poorly understood. Here, we report the function and role of Antioxidant Protein1 (OsATX1), a Cu chaperone in rice (Oryza sativa). Knocking out OsATX1 resulted in increased Cu concentrations in roots, whereas OsATX1 overexpression reduced root Cu concentrations but increased Cu accumulation in the shoots. At the reproductive stage, the concentrations of Cu in developing tissues, including panicles, upper nodes and internodes, younger leaf blades, and leaf sheaths of the main tiller, were significantly increased in OsATX1-overexpressing plants and decreased in osatx1 mutants compared to that in wild type...
July 12, 2018: Plant Physiology
Aurélie Chambon, Allan West, Daniel Vezon, Christine Horlow, Arnaud De Muyt, Liudmila Chelysheva, Arnaud Ronceret, Alice R Darbyshire, Kim Osman, Stefan Heckmann, F Christopher H Franklin, Mathilde Grelon
During the leptotene stage of prophase I of meiosis, chromatids become organized into a linear looped array via a protein axis that forms along the loop bases. Establishment of the axis is essential for the subsequent synapsis of the homologous chromosome pairs and the progression of recombination to form genetic crossovers. Here, we describe ASYNAPTIC4 (ASY4), a meiotic axis protein in Arabidopsis thaliana. ASY4 is a small coiled-coil protein that exhibits limited sequence similarity with the C-terminal region of the axis protein ASY3...
July 12, 2018: Plant Physiology
Timothy J Hearn, Maria C Marti, S M Abdul-Awal, Rinukshi Wimalasekera, Camilla R Stanton, Michael J Haydon, Frederica L Theodoulou, Matthew A Hannah, Alex Ar Webb
Circadian clocks drive rhythms with a period near 24 hours, but the molecular basis of the circadian period's regulation is poorly understood. We previously demonstrated that metabolites affect the free-running period of the circadian oscillator of Arabidopsis thaliana, with endogenous sugars acting as an accelerator and exogenous nicotinamide acting as a brake. Changes in circadian oscillator period are thought to adjust the timing of biological activities through the process of entrainment, in which the circadian oscillator becomes synchronised to rhythmic signals such as light and dark cycles, as well as changes in internal metabolism...
July 11, 2018: Plant Physiology
Alexandra Baekelandt, Laurens Pauwels, Zhibiao Wang, Na Li, Liesbeth De Milde, Annelore Natran, Mattias Vermeersch, Yunhai Li, Alain Goossens, Dirk Inzé, Nathalie Gonzalez
In Arabidopsis, reduced expression of the transcriptional regulator PEAPOD2 (PPD2) results in propeller-like rosettes with enlarged and dome-shaped leaves. However, the molecular and cellular processes underlying this peculiar phenotype remain elusive. Here, we studied the interaction between PPD2 and NOVEL INTERACTOR OF JAZ (NINJA) and demonstrated that ninja loss-of-function plants produce rosettes with dome-shaped leaves similar to those of ppd mutants, but without the increase in size. We showed that ninja mutants have a convex-shaped primary cell cycle arrest front, putatively leading to excessive cell division in the central leaf blade region...
July 10, 2018: Plant Physiology
Rachel Maczis Shahan, Christopher Zawora, Haley Melissa Wight, John Sittmann, Wanpeng Wang, Stephen M Mount, Zhongchi Liu
Fragaria vesca, a diploid strawberry, is a developing model system for the economically important Rosaceae family. Unlike tomato, the traditional model for fleshy fruit development, strawberry fleshy fruit develops from the floral receptacle and its ripening is non-climacteric. Additionally, the external seed configuration of strawberry fruit facilitates the study of seed-to-fruit cross-tissue communication, particularly phytohormone biosynthesis and transport. Insight gained from strawberry fruit development broadens our understanding of general developmental processes...
July 10, 2018: Plant Physiology
Dario Leister
No abstract text is available yet for this article.
July 10, 2018: Plant Physiology
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