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Mutation Research

Carolina S Stein, José A M de Carvalho, Marta M M F Duarte, Ivana B M da Cruz, Melissa O Premaor, Fabio V Comim, Maria B Moretto, Rafael N Moresco
Uric acid presents different roles in an organism, since it can act as an antioxidant or a pro-oxidant molecule. High serum uric acid levels may cause damage to several structures, including nucleic acids and its components. Therefore, in this study the association between increased serum uric acid concentrations and oxidation of nucleosides was investigated by assessment of urinary 8-hydroxydeoxyguanosine (8-OHdG) in patients with type 2 diabetes (T2D) and in healthy individuals. Urinary 8-OHdG and biochemical parameters were assessed in 61 patients who were initially grouped into 2 groups based on the median serum uric acid levels (<5...
September 22, 2018: Mutation Research
Heloísa Lizotti Cilião, Rossana Batista Oliveira Camargo-Godoy, Marilesia Ferreira de Souza, Amanda Zanuto, Vinicius Daher Alvares Delfino, Ilce Mara de Syllos Cólus
The immunosuppressant mycophenolic acid (MPA), derived from the prodrug mycophenolate mofetil (MMF), is a drug used widely by kidney transplant recipients. This drug selectively inhibits inosine monophosphate dehydrogenase that controls the proliferation of lymphocytes, aiding in the prevention of rejection of the transplanted organ. Polymorphisms in key genes involved in MMF metabolism may alter the function of the enzymes encoded by them and contribute to interindividual variability in the response to the drug and its efficacy...
December 2018: Mutation Research
João Paulo de Castro Marcondes, Pablo Felipe Bertolini Andrade, André Luiz Ventura Sávio, Maruhen Amir Datsch Silveira, Marilza Vieira Cunha Rudge, Daisy Maria Fávero Salvadori
Several findings suggest that in utero stressor stimuli can alter fetal development by promoting transcriptional changes, and predisposing the neonate to diseases later in life. This study aimed to investigate whether a hyperglycemic environment in pregnant women with gestational diabetes mellitus (GDM) is able to cause fetal genetic alterations and predispose neonates to obesity. Transcriptional alteration of SIRT1, TP53 and BCL2 genes, miR-181a (a SIRT1 or BCL2 regulator) and telomere length were evaluated in placental and umbilical-cord blood cells...
December 2018: Mutation Research
Vivian Francilia Silva Kahl, Daniel Simon, Melissa Rosa de Souza, Vieira Henrico da Rosa, Caroline Nicolau, Fernanda R Da Silva, Katia Kvitko, Alessandra Peres, Gilson Pires Dorneles, Cláudia Telles de Souza, Johnny Ferraz Dias, Juliana Da Silva
Pesticides are one of the most frequently investigated chemical, due to their multiple uses in agricultural and public health areas. This study evaluates lymphocytes CBMN (cytokinesis-block micronucleus cytome assay), inflammatory markers, inorganic elements in blood samples, and the relationship of these parameters with XRCC1Arg194Trp, OGG1Ser326Cys and PON1Gln192Arg polymorphisms in a population of tobacco farmers. The study population comprised 129 agricultural workers exposed to pesticides and 91 nonexposed...
December 2018: Mutation Research
Elisa E Córdoba, Ezequiel Lacunza, Martín C Abba, Eduardo Fernández, Alba M Güerci
Although oncological therapies have improved in the last decades, breast cancer (BC) remains a serious health problem worldwide. Radiotherapy (RT) is one of the most frequently used treatments for cancer aimed at eliminating tumor cells. However, it can also alter the surrounding normal tissue, especially the skin, and patient reactions may vary as a result of extrinsic and intrinsic factors. We evaluated the association of gene polymorphisms ATM Asp1853Asn, IL-6 G-174C and TNF-α G-308A involved in central phenotype pathways and development of individual radiosensitivity in BC patients with an exacerbated response to RT...
December 2018: Mutation Research
Yuliana C Banegas, Eliana E Ocolotobiche, Gisel Padula, Elisa E Córdoba, Eduardo Fernández, Alba M Güerci
Radiotherapy is widely used for cancer treatment. However, its adverse effects that may develop during the course of treatment have forced to search agents to protect biological systems against the deleterious effects of radiation. Resveratrol (3,4,5-trihydroxy-trans-stilbene; RSV) is a natural polyphenol currently promoted for its beneficial pleiotropic effects on health, which has been shown to exhibit antioxidant properties while inhibiting the growth of tumor cells. In radioresistant tumors, RSV could contribute to reduce recurrence and treatment failure...
December 2018: Mutation Research
Marcela M López Nigro, Roxana N Peroni, Iván Ayllón-Cabrera, Victoria E Schiariti Lampropulos, Martín I Roma, Marta A Carballo
Several epidemiological studies have demonstrated that a diet with high contents of cruciferous vegetables (which belong to the Brassicaceae family) may reduce the incidence of cancer and neurodegenerative diseases. However, some authors have postulated that they might bring about toxic effects. Therefore, the aim of this study was to assess the effects of chronic administration of Diplotaxis tenuifolia (wild rocket), a species found in Argentina, concerning its putative genotoxicity or antigenotoxicity against the DNA damage inducer cyclophosphamide, and its ability to modulate the hepatic expression of ABC efflux transporters on mice...
December 2018: Mutation Research
Emilene A Nunes, Tânia M Manieri, Andreza C Matias, Fernanda R Bertuchi, Daniela A da Silva, Larissa Lago, Roseli H Sato, Giselle Cerchiaro
In this work, we aim to provide evidence for the protective effect of a copper chelator, neocuproine (NeoCu), against the oxidative stress in NSC34 cells, which inhibits biomolecule oxidation and cell death. Results obtained with the comet assay allowed to determine the increase in oxidized purines and pyrimidines by H2 O2 exposure, and their changes after the addition of NeoCu. We also observed a higher ATP7b activity in nuclei and a higher Cu concentration inside the cells, proving that the NeoCu acts directly in DNA to promote cell recovery in oxidative stress conditions, also observed in Reactive Oxygen Species (ROS) detection assay by Flow Cytometry...
December 2018: Mutation Research
Willian Orlando Castillo, Andres Felipe Aristizabal-Pachon, Elsa Sakamoto-Hojo, Cristian Aldemar Gasca, Fabio Antonio Cabezas-Fajardo, Catarina Takahashi
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neuritic plaques (NPs), and neurofibrillary tangles (NFTs). β-Amyloid peptide 1-4 2 (Aβ(1-42) ) is the principal component of NPs and is associated with oxidative stress, as well as dysfunction of cholinergic neurotransmission system and cell death. Nevertheless, one of the most promising therapeutic approaches for patients with AD is based on the pharmacological intervention to increases acetylcholine levels and reduces oxidative stress in AD brain...
December 2018: Mutation Research
Raíne F De Carli, Débora Dos S Chaves, Tatiane R Cardozo, Ana Paula de Souza, Allan Seeber, Wladimir H Flores, Karol F Honatel, Mauricio Lehmann, Rafael R Dihl
Nickel-based nanoparticles (NPs) are new products with an increasing number of industrial applications that were developed in recent years. NiO NPs are present in several nanotechnological industrial products, and the characterization of their genotoxic potential is essential. The present study assessed the genotoxicity of NiO NPs in vivo and in vitro using the somatic mutation and recombination test in somatic cells of Drosophila melanogaster (SMART), the cytokinesis - block micronucleus assay (CBMN), and the comet assay in a V79 cell line...
December 2018: Mutation Research
Willian Robert Gomes, Paula Pícoli Devóz, Bruno Limonti Cano Luiz, Denise Grotto, Bruno Lemos Batista, Fernando Barbosa, Gustavo Rafael Mazzaron Barcelos
Individual susceptibility to the toxic effects induced by exposure to lead (Pb) may be affected by several variables, such as environmental factors, as well as intrinsic variations among the individuals, which are hypothetically associated to genetic differences in enzymes metabolizing the metal. Aim of the present study was to evaluate the effects of polymorphisms of glutathione (GSH)-genes related to the antioxidant status and Pb metabolism (GCLC, rs17883901 and GCLM, rs41303970) on Pb levels in blood (B-Pb) and plasma (P-Pb), as well as Pb-related effects on activity of glutathione-peroxidase (GPX) and on GSH concentrations...
December 2018: Mutation Research
Catarina S Takahashi
The Asociación Latinoamericana de Mutagénesis, Carcinogénesis y Teratogénesis Ambiental (ALAMCTA) is the organizational structure encompassing the five national environmental mutagenesis societies of Latin America. It was founded in 1980 and has held 10 congresses and had 10 presidents, representing members from throughout Latin America. This brief review describes the founding of ALAMCTA and the key events associated with it, including the initiation in 1993 of the influential Alexander Hollaender Courses in Mexico City, and the hosting of the 11th International Conference on Environmental Mutagens (ICEM) in 2013 in Foz do Iguaçu, Brazil...
December 2018: Mutation Research
Melissa Rosa de Souza, Vivian Francília Silva Kahl, Paula Rohr, Kátia Kvitko, Mónica Cappetta, Wilner Martinez Lopes, Juliana da Silva
Coal is a mixture of several chemicals, mainly inorganic elements and polycyclic aromatic hydrocarbons, many of which have mutagenic and carcinogenic effects. Pneumoconiosis, fibrosis, asbestosis, silicosis, emphysema, loss of lung function and cancer are some examples of coal-related disorders. The aim of this study was to analyze coal miners with respect to telomere length (TL) and percentage (%) of global DNA methylation. The study involved 82 participants divided into two groups: 55 workers exposed to coal and 27 non-exposed individuals...
December 2018: Mutation Research
Lyda Espitia-Pérez, Juliana da Silva, Hugo Brango, Pedro Espitia-Pérez, Karina Pastor-Sierra, Shirley Salcedo-Arteaga, Claudia T de Souza, Johnny F Dias, Luz Stella Hoyos-Giraldo, Miguel Gómez-Pérez, Daniela Salcedo-Restrepo, João A P Henriques
DNA and chromosomal damage in individuals occupationally exposed to coal mining residues have repeatedly been reported in lymphocytes and epithelial cells, suggesting a systemic exposure-response in which generation of oxidative damage may play a major role. Nevertheless, the understanding of this mechanism is still incomplete, particularly in regard to environmental exposures. This study aimed to evaluate DNA damage using the cytome assay (BMN-cyt) in buccal cells and its relation to primary and oxidative DNA damage in lymphocytes, assessed by the high-throughput alkaline and modified (FPG-ENDO III) Comet assay in individuals with environmental exposure to coal mining residues in northern Colombia...
December 2018: Mutation Research
Gustavo A Folle
No abstract text is available yet for this article.
December 2018: Mutation Research
Deidamia Franco de Diana, Jaime Segovia Abreu, Diana Castiglioni Serafini, José Francisco Ortíz, María José Samaniego, Ana Carina Aranda, Enrique Zamorano-Ponce
Cateura landfill located in Asunción-Paraguay is the site of final disposal of tons of garbage of all kinds coming from urban, industrial and commercial areas. Due to an inadequate waste management system, there is a big concern in our country on how it can affect people working within the landfill as waste pickers. When a high risk of exposure affecting workers in their work places is supposed, defining biomarkers of genotoxic damage is obligatory. The principal aim of this study was to evaluate the genetic damage in occupationally exposed women in their work environments through two established biomarkers: the single-cell gel electrophoresis assay (SCGE) accomplished in peripheral blood and the micronucleus test performed in exfoliated cells from oral mucosa (BMCyT)...
December 2018: Mutation Research
Annabel Quinet, Leticia K Lerner, Davi J Martins, Carlos F M Menck
During DNA replication, forks may encounter unrepaired lesions that hamper DNA synthesis. Cells have universal strategies to promote damage bypass allowing cells to survive. DNA damage tolerance can be performed upon template switch or by specialized DNA polymerases, known as translesion (TLS) polymerases. Human cells count on more than eleven TLS polymerases and this work reviews the functions of some of these enzymes: Rev1, Pol η, Pol ι, Pol κ, Pol θ and Pol ζ. The mechanisms of damage bypass vary according to the lesion, as well as to the TLS polymerases available, and may occur directly at the fork during replication...
December 2018: Mutation Research
Roberta Passos Palazzo, Laura Bannach Jardim, Alexandre Bacellar, Fernanda Ramos de Oliveira, Flora Troina Maraslis, Carolina Hilgert Jacobsen Pereira, Juliana da Silva, Sharbel Weidner Maluf
Ataxi A-T elangiectasia (AT) is a multisystem, complex and rare disease inherited in an autosomal recessive manner. Homozygous individuals have a variety of pathological manifestations, however, heterozygotes only present a higher risk of developing cancer. We evaluated the background levels of DNA damage (basal damage) and cell response to bleomycin or ionizing radiation using Comet assay and the cytokinesis-block micronucleus (CBMN) test in individuals with AT, their parents and controls. To evaluate DNA repair, the challenge experiment with ionizing radiation was performed using Comet assay, and different recovery times were evaluated...
December 2018: Mutation Research
Ana Laura Reyes-Ábalos, Pablo Liddle, Gustavo A Folle, María Vittoria Di Tomaso
DNA damage response (DDR) constitutes a protein pathway to handle eukaryotic DNA lesions in the context of chromatin. DDR engages the recruitment of signaling, transducer, effector, chromatin modifiers and remodeling proteins, allowing cell cycle delay, DNA repair or induction of senescence or apoptosis. An early DDR-event includes the epigenetic phosphorylation of the histone variant H2AX on serine 139 of the C-termini, so-called gammaH2AX. GammaH2AX foci detected by immunolabeling on interphase nuclei have been largely studied; nonetheless gammaH2AX signals on mitotic chromosomes are less understood...
December 2018: Mutation Research
Kalpana Gopalakrishnan, Shriram Venkatesan, Esther Su Hui Low, M Prakash Hande
The mTOR pathway and the enzyme telomerase are two key players commonly upregulated in cancers. They render survival and proliferative advantage to cancer cells, and are regarded as attractive anticancer targets. Rapamycin, a macrolide antibiotic and mTOR inhibitor, has recently also been implicated in telomerase inhibition and telomere attrition, although the mechanisms remain poorly understood. Using breast cancer cells (MCF-7 and MDA-MB-231) wherein telomerase activity and mTOR pathway are concurrently overexpressed, this study sought to unravel novel mechanisms by which rapamycin may affect these pathways...
December 2018: Mutation Research
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