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L Chen, M Liu, J Ji, W Lin, F Shan, H Liu
To evaluate the diagnostic accuracy of peripheral blood kisspeptin (KISS1) mRNA and plasma cancer antigen 125 (CA125) protein of epithelial ovarian cancer (EOC) in previously pregnant patients, we prospectively enrolled 40 EOC patients as cases and 20 uterine fibroids patients with normal ovary as controls. Levels of peripheral blood KISS1 mRNA and plasma CA125 protein was respectively measured by RT-PCR and electrochemiluminescent method, respectively. Receiver operating characteristic curves with area under curve (AUC) were used to evaluate the diagnostic accuracy...
September 6, 2016: Neoplasma
Z Y Wang, W Zhang, J J Yang, D K Song, J X Wei, S Gao
The clinical significance of thymosin β4 (Tβ4) expression in bladder transitional cell carcinoma (BTCC) remains unclear. The present study assessed the relationship between the expression of Tβ4 protein and the clinicopathological features, as well as the prognosis of bladder cancer patients. Tβ4 protein expression in 24 normal bladder and 138 primary BTCC tissue specimens was detected by immunohistochemistry, and the association of this expression with BTCC clinicopathological features and recurrence as well as patient survival was analyzed...
September 6, 2016: Neoplasma
J Liu, J Wang, Y Song, Y Yang, Y Hu, P Gao, J Sun, X Chen, Y Xu, Z Wang
Long non-coding RNAs (lncRNAs) occupy the majority of the human genome. Their dysregulation is usually related with the procession of diseases, including tumors. Abnormally expressed lncRNAs have been found in colorectal cancer (CRC), but are not fully understood. In the current study, we determined the expression level of a novel lncRNA-RP1-13P20.6 in 99 pairs of CRC tissues compared to their matched normal adjacent tissues, using real time polymerase chain reaction. We further assessed the correlation between their expression levels and their clinicopathological parameters, and determined the potential of RP1-13P20...
September 6, 2016: Neoplasma
Q Li, Y Dai, F Wang, S Hou
Colorectal cancer (CRC) is a disease with high incidence, especially in developed countries. Long non-coding RNAs (lncRNAs) are new research hotspots for their vital roles in regulating gene expression. This study aims to investigate the prognostic value of lncRNAs in CRC patients. A total of 21 cancer-related lncRNAs were detected by PCR array to reveal their expression changes in CRC tissue. A 120-week-long follow-up was performed in 30 CRC patients to analyze the relationship between lncRNA levels and CRC prognosis...
September 6, 2016: Neoplasma
Y Lan, D Liu, M Lin
Urinary bladder cancer is one of the most frequent cancers worldwide. Non-muscle-invasive bladder carcinoma (NMIBC) has a very low curative rate after resection. The meta-analysis aims to compare efficiency of the combination therapy of bacillus Calmette-Guérin (BCG) and mitomycin C (MMC) with each monotherapy on NMIBC treatment. Articles were retrieved in relevant databases up to May, 2016. The Cochrane Collaboration Risk of Bias Tool was used to assess quality of the included studies. Risk ratio (RR) and the 95% confidence interval (CI) were used as the effect size to calculate pooled result...
September 6, 2016: Neoplasma
N Toshikuni, Y Matsue, T Minato, N Hayashi, M Tsutsumi
The transcriptional activity of transforming growth factor-β (TGF-β) is increased in subjects with hepatocellular carcinoma (HCC). Recent studies have indicated that the -509C genotype in hepatitis B virus (HBV)-infected subjects and the -509T genotype in hepatitis C virus (HCV)-infected subjects can increase the transcriptional activity of the TGF-β1 gene. We conducted a meta-analysis to clarify whether these two hepatitis viruses affect the association between TGF-β1 C-509T variants and HCC susceptibility...
September 6, 2016: Neoplasma
M Traczyk-Borszynska, E Borkowska, Z Jablonowski, A Jedrzejczyk, M Pietrusinski, B Kaluzewski, M Sosnowski, M Borowiec
The aim of the study was to assess the genetic diversity of bladder cancer and determine the suitability of a proposed molecular marker panel to monitor the course of bladder cancer patients. The study involved 185 patients with diagnosed bladder cancer. The genetic diversity of the bladder cancer was evaluated by the prevalence of mutations in the TP53, HRAS, FGFR3 and WWOX genes. Mutations were detected in 62.2% of the tumor samples. The most frequently mutated genes were FGFR3 (49.7%) and TP53 (16.2%). No mutation was observed in the WWOX gene...
September 6, 2016: Neoplasma
D Jurkovicova, M Magyerkova, Z Sestakova, L Copakova, V Bella, M Konecny, M Krivjanska, L Kulcsar, M Chovanec
Breast cancer (BC) including its progression into bone metastasis is a complex process involving changes in gene expression and function of both, microRNAs (miRNAs) and their target genes. Deregulation of miRNAs has been described as a crucial factor responsible for the initiation and progression of BC, and specific miRNA expression profiles have been found to be associated with particular disease states, histological tumor types, and BRCA1/2 or HER status. BRCA1 tumor suppressor is involved in DNA damage response and repair and epigenetically controls miR-155 expression and its pro-cancerous potential...
September 6, 2016: Neoplasma
J Chen, L Lai, S Liu, C Zhou, C Wu, M Huang, Q Lin
Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor to initiate the expressions of distinct pro-angiogenic growth genes, particularly the expression of vascular endothelial growth factor (VEGF).CoCl2 was used in rat liver tumor cell line McA RH-7777 to stimulate hypoxia to mimic the hypoxic conditions induced by transcatheter arterial chemoembolization (TACE). CCK8 assays were performed to examine the effect of hypoxia on cell viability. Real-time qRT-PCR, western blot and ELISA assays were used to measure the expression of HIF-1α and VEGF in McA RH-7777 cells under hypoxic conditions, respectively...
August 27, 2016: Neoplasma
U Altanerova, K Benejova, V Altanerova, S Tyciakova, B Rychly, P Szomolanyi, F Ciampor, M Cihova, V Repiska, K Ondicova, B Mravec, C Altaner
We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nanoparticles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0...
August 27, 2016: Neoplasma
M Adelipour, A Allameh, S M Tavangar, Z M Hassan, M Soleimani
Incorporation of endothelial cells or their progenitor cells into newly sprouting blood vessels can contribute to tissue vascularization after ischemic injury. However, the interaction of the stem cells-derived endothelial cells with angiogenesis within tumors is not well understood. The aim of this study was to examine the efficiency of endothelial-like cells derived from MSCs in controlling breast tumor growth associated with abnormal angiogenesis. For this purpose, Balb/c mouse model of breast carcinoma was developed and subjected to cell intra tumor therapy (I...
August 27, 2016: Neoplasma
B Smolkova, S Miklikova, V Horvathova Kajabova, A Babelova, N El Yamani, M Zduriencikova, I Fridrichova, I Zmetakova, T Krivulcik, L Kalinkova, M Matuskova, L Kucerova, M Dusinska
Epithelial-to-mesenchymal transition (EMT) significantly affects the risk of metastasising in breast cancer. Plasticity and reversibility of EMT suggest that epigenetic mechanisms could be the key drivers of these processes, but little is known about the dynamics of EMT-related epigenetic alterations. We hypothesised that EMT, mediated by autocrine and paracrine signals, will be accompanied by changes in DNA methylation profiles. Therefore, conditioned medium from adipose tissue-derived mesenchymal stromal cells was used for induction of EMT in human breast cancer SK-BR-3 cell line...
August 27, 2016: Neoplasma
C Zhang, W Gao, S Wen, Y Wu, R Fu, D Zhao, X Chen, B Wang
To uncover potential key genes, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) associated with laryngeal squamous cell carcinoma (LSCC), microarray data of mRNA, miRNAs and lncRNAs produced from matched sample pairs of LSCC and adjacent normal samples were used to this analysis. Differentially expressed genes (DEGs), miRNAs (DE-miRNAs) and lncRNAs (DE-lncRNAs) were identified, and functions and correlations of them were analyzed. In total, 826 DEGs, 44 DE-miRNAs and 347 DE-lncRNAs were identified. The up-regulated DEGs were mainly related to cell cycle, and the down-regulated DEGs were correlated with regulation of biological quality and extracellular region...
August 27, 2016: Neoplasma
X Z Li, Y L Sun, L Q Cao, M J Li
The presented study aimed to investigate the antitumor efficacy of combination of oxaliplatin with rapamycin, an mTOR inhibitor, in hepatocellular carcinoma (HCC). The activation status of mTOR pathway was first examined in HCC cell lines HepG2, BEL7402, and HuH7 using Western blotting. Effects of rapamycin, oxaliplatin, and their combination on the proliferation of HCC cells were determined in vitro using MTT assay and in vivo using a nude mice model bearing HepG2 xenografts. Drug-induced cell apoptosis was examined by flow cytometry...
August 27, 2016: Neoplasma
S Hong, M Bi, S Chen, P Zhao, B Li, D Sun, J Tai
MicroRNAs (miRNAs) are small, non-coding RNAs that can serve as tumor suppressor genes or oncogenes in tumorigenesis. More and more evidence demonstrate that abnormal expression of miRNAs lead to the gastric carcinoma occurrence. In the present study, we revealed that the expression levels of miR-520f were significantly down-regulated in gastric carcinoma cells and clinical gastric carcinoma samples. Next, we demonstrated that introduction of miR-520f inhibited the growth of gastric carcinoma cells in vitro...
August 27, 2016: Neoplasma
A Zou, R Liu, X Wu
Ovarian cancer is a gynecological malignancy worldwide. Long non-coding RNAs (lncRNAs) research is an emerging area in cancer studies, but little is known about lncRNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in ovarian cancer. This study aims to investigate expression and roles of MALAT1 in ovarian cancer. MALAT1 level was detected in 20 ovarian cancer patients. MALAT1 expression was promoted by transforming growth factor β1 (TGFB1) treatment and inhibited by siRNA transfection in human ovarian cancer cell line SK-OV-3, after which changes in cell viability, proliferation, migration and invasion were analyzed by MTT, colony formation and Transwell assays...
August 27, 2016: Neoplasma
B Xu, H Zhao, C Xu
This study aimed to investigate the role and potential mechanism of miR-22 in clear cell ovarian cancer (CCOC) progression. The gene expression profile of GSE16568, including 3 CCOC samples with miR-22 overexpression and 3 negative controls, was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were screened using the limma package in R. Gene Ontology (GO) and pathway enrichment analysis of DEGs were performed by using The Database for Annotation, Visualization and Integrated Discovery (DAVID)...
August 27, 2016: Neoplasma
S Hong, M Bi, Z Yan, D Sun, L Ling, C Zhao
Colorectal cancer is one of the most common malignant tumors with a high rate of distant metastasis, postoperative recurrence and mortality. ATPase family AAA domain-containing protein 2 (ATAD2), a member of ATPase family, is highly expressed in various cancers, including colorectal cancer. However, whether ATAD2 plays a role in the migration and invasion of colorectal cancer cells remains unknown. In this study, we established ATAD2 knockdown in colorectal cancer cell lines by RNA interference and found that silencing of ATAD2 inhibited the migration and invasion ability of Caco-2 and SW-480 cells...
August 27, 2016: Neoplasma
E Panisova, M Kery, J Kopacek, S Pastorekova, E Svastova
Cancer cells often rely on glycolytic metabolism in order to fulfill high demands of ATP and macromolecules for the sustained growth and proliferation. However, glycolysis is not necessarily the main source of energy for all cancer cells. Some of them rather depend on glutamine or lactate that favor the utilization of oxidative metabolic pathway. Different employment rate of metabolism creates variable products that participate in the formation of environmental milieu, which in turn triggers broad spectrum of cellular signaling pathways leading to migration, invasion, or proliferation...
August 27, 2016: Neoplasma
X Han, L Zhao, X Li
Helicase POLQ-like (HELQ), an ATP-dependent 3'-5' DNA helicase, plays pivotal roles in DNA processing, such as DNA replication, recombination and repair. The recent discovery of HELQ in mouse model and human cancer cell reveal that genetic alteration of HELQ is involved in cancer susceptibility and subfertility. Here we review the origin, function and mechanisms of HELQ in maintaining genomic stability. Furthermore, we also summarize the known roles of HELQ in human carcinogenesis and reproduction. Importantly, we review that HELQ releases DNA replication block at interstrand cross-linking sites in cellular tolerance to cross-linking agents during DNA repair process...
August 27, 2016: Neoplasma
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