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J M Zhao, W Cheng, X G He, Y L Liu, F F Wang, Y F Gao
Lung cancer remains the most common cause of tumor-related death worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in the development of various cancers, including lung cancer. This study aimed to investigate the effect and the molecular basis of lncRNA PICART1 on lung cancer. We first assessed the PICART1 expression in lung cancer in vitro and vivo by qRT-PCR. Then the expression of PICART1 in SPC-A-1 and NCI-H1975 cell lines was inhibited and overexpressed by transient transfections...
June 26, 2018: Neoplasma
L Tian, Z Z Tao, H P Ye, G Y Li, Z F Zhan, H W Tuo
The early-stage diagnosis and treatment for the recurrence of larynx carcinoma needs further investigation. Mesenchyme homeobox 2 (MEOX2) was speculated as a novel suppressor gene in larynx carcinoma in our study, the molecular mechanism was studied. Real-time quantitative PCR (RT-qPCR) and Western blot were used to detect mRNA and protein levels of MEOX2 in laryngeal cancer tissues and cells (Hep-2, TU212, AMC-NH-8 and TU686 cells), and also apoptosis and phosphoinositide 3-kinase (PI3K)/protein kinase (Akt) related factors in TU212 cells transfected with MEOX2...
June 26, 2018: Neoplasma
R Sobotka, O Capoun, T Hanus, T Zima, M Kalousova, V Soukup
To assess the prognostic importance of serum levels of osteopontin (OP), stanniocalcin-1 (SC), FGF-23, alpha Klotho and 25-OH-D at the time of diagnosis in renal cell carcinoma (RCC) patients a total of 56 RCC patients with staging ≥ pT1b were enrolled in a prospective study. The relationship between the serum level of the analysed parameters and recurrence-free survival (RFS), overall survival (OS) and cancer-specific survival (CSS) was examined. Control group consisted of 20 patients without cancer. Level of osteopontin, stanniocalcin-1, FGF-23 and alpha Klotho were determined by Enzyme-Linked Immunosorbent Assay (ELISA), 25-OH-D by chemiluminiscence immunoanalysis (CLIA)...
June 26, 2018: Neoplasma
X M Lu, H Long
Cancer is the main leading cause of death in the world, although it has been made noteworthy advances in cancer research in the past decades. Early detection of cancer is extremely important in improving the chances of successful therapy. Thus, it is urgently needed to make further efforts to explore novel tumor markers for treatment.Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme which catalyzes the N-methylation of nicotinamide to form 1-methylnicotinamide (1-MNA), and plays an important role in controlling the intracellular concentration of nicotinamide...
June 26, 2018: Neoplasma
S K Chang, J Hou, G G Chen, D D Yu, H Q Wu, Y S Xie, L N Hu, L Gao, W Q Xiao, Y Y Kong, J M Shi
Natural killer (NK) cell-based immunotherapy is promising, as NK cells are in the first line of defense against cancer and capital of lysing tumor cells without pre-stimulation. However, NK cells from multiple myeloma (MM) patients are always deficient in numbers and the expression of certain activating receptors, disabling them in cytotoxicity against the cancer. Therefore, effective strategies to expand NK cells and increase NK cell-mediated cytotoxicity against MM are significant. Here, NK cells were efficiently expanded from peripheral blood mononuclear cells (PBMCs) of newly diagnosed MM patients after co-culture with irradiated K562 cells transfected with 41BBL and membrane-bound interleukin (IL)-15 (K562-mb15-41BBL) in the presence of 200 IU/ml human IL-2...
June 26, 2018: Neoplasma
K Hasakova, M Vician, R Reis, M Zeman, I Herichova
Colorectal cancer (CRC) exhibits differences in its features depending on the location of the tumor. The role of the circadian system in the carcinogenesis is accepted and many studies report different clock gene expression in tumors compared to healthy tissue, although a little attention is given to the changes in clock genes in tumors arising from various locations across the colon and rectum. The aim of our study was to investigate the expression of the clock genes cry1 and cry2 in human CRC tissue and tissue adjacent to colorectal tumors in a cohort of 64 patients by real time PCR...
June 26, 2018: Neoplasma
K Vicikova, E Petrovcikova, P Manka, J Drach, H Stockinger, V Leksa
The mannose 6-phosphate/insulin-like growth factor 2 receptor (CD222, M6P/IGF2R) is a multifunctional transmembrane type I receptor, mostly localized intracellularly, less on the surface of all types of mammalian cells. It is known both to transport lysosomal enzymes through their mannose 6-phosphate moieties and to internalize extracellular ligands like insulin-like growth factor 2 or plasminogen. CD222 is involved in regulation of cell proliferation, migration, T cell activation, and apoptosis. Soluble CD222 has been found in higher concentrations in sera of liver disease patients...
June 26, 2018: Neoplasma
S Q Feng, X Y Zhang, H T Fan, Q J Sun, M Zhang
It was proved that maternally expressed 3 (MEG3), a long non-coding RNA (LncRNA), is down-regulated and inversely correlated with the prognosis in various types of cancer, including bladder cancer (BC). Nevertheless, the role of MEG3 in BC has not been fully elaborated. Here, we found that MEG3 expression was reduced in 21 BC tumor tissue samples compared with the corresponding adjacent tissues. Then, we established T24 and 5637 cells with a stably integrated expression of MEG3 by G418 resistance screening...
June 26, 2018: Neoplasma
G Helbig, A Armatys, K Boral, A J Kopinska, K Wozniczka, M Dworaczek, K Chromik, A Koclega, M Panz-Klapuch, M Wysocka, A Janikowska
The incorporation of pegylated asparaginase (PEG-ASP) into pediatric and adult acute lymphoblastic leukemia (ALL) protocols remains a worldwide therapeutic standard, however the safety profile is a challenge. We herein report, in adults, the toxicity of intravenous single dose of 1000 IU/m2 PEG-ASP administered in remission induction for ALL patients. Thirty two patients at median age at diagnosis of 32 years (median 19-65) were included in this analysis. Most patients had B-cell lymphoblastic leukemia (n=26; 78%) and 81% of cases were dministration to first toxic symptoms was 7 days (range 1-19)...
June 26, 2018: Neoplasma
J Wang, X Q Chu, D Zhang, D F Kong
Gastric cancer is the main leading cause of cancer-related death worldwide. The aberrant expression of paternally expressed gene 10 (PEG10) is involved in development of a range of cancers. However, the potential biological function and the underling mechanism of PEG10 in human gastric carcinoma are still unknown. Knocking down LncRNA PEG10 might represent a promising therapeutic strategy for the treatment of gastric cancer. The expression of PEG10, miR-3200, and AEG1 in human gastric carcinoma NCI-N87 cells were altered by cell transfection assay...
June 26, 2018: Neoplasma
W J Wu, H Yin, J J Hu, X Z Wei
Mounting evidence indicates that long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. LncRNA LINC00313 has been found to be upregulated and associated with the poor prognosis of lung cancer. However, the potential role and clinical value of LINC00313 in human papillary thyroid cancer (PTC) remain elusive and need to be examined. The aim of the present study was to investigate the role of LINC00313 in PTC. We found that the expression of LINC00313 was significantly upregulated in PTC tissues and cell lines and that this upregulation was correlated with a poor prognosis...
June 26, 2018: Neoplasma
R Vrba, D Vrana, C Neoral, R Aujesky, M Stasek, T Bohanes, J Tesarikova, J Cincibuch, B Melichar, M Horakova, J Zapletalova
A single-center retrospective study the complication and mortality of surgical treatment of esophageal cancer 2006 to 2015 is presented. A total of 212 patients with esophageal cancer were operated at the First Department of Surgery University Hospital Olomouc, Czech Republic in the period between 2006 and 2015. Adenocarcinoma was histologically described in 127 patients (59.9%), squamous cell carcinoma in 82 patients (38.7%), and other types of carcinoma were described in 3 cases. According to the preoperative staging of esophageal cancer, the patients with early stage disease (T1-2N0M0) had primary surgery, while the patients with advanced stage (T3-4N0-2M0) were indicated for neoadjuvant chemoradiation with the surgery being performed subsequently...
June 26, 2018: Neoplasma
C X Zeng, L Y Tang, C Y Xie, F X Li, J Y Zhao, N Jiang, Z Tong, S B Fu, F J Wen, W S Feng
The homology of epidermal growth factor receptor pathway substrate 8 (EPS8), EPS8L3, is elevated significantly in hepatocellular carcinoma (HCC) tissues and cell lines compared with the normal liver tissues and cell lines. The MTT and colony formation assays demonstrated that overexpressing EPS8L3 enhances, while silencing reduces the proliferation of HCC cells. Further experiments illustrated that overexpressing EPS8L3 promotes the expression of p-AKT, Cyclin D1, but inhibits the transcriptional activity of FOXO1...
June 26, 2018: Neoplasma
J X Yang, Y Sun, L Gao, Q Meng, B Y Yang
Glioma is among the most fatal brain tumors characterized by a highly malignancy and rapid progression and early metastasis. Dysregulation of long non-coding RNA differentiation antagonizing non-protein coding RNA (LncRNA DANCR) is associated with the development, progression and metastasis of various cancers. In the present study, we investigated functional role of LncRNA DANCR in the malignancy of glioma. The results showed that LncRNA DANCR was increased in glioma tissues and cells compared with normal brain tissues and cells...
June 26, 2018: Neoplasma
X F Ni, L H Zhao, G Li, M Hou, M Su, C L Zou, X Deng
Nuclear receptor interacting protein (NRIP1), also known as RIP140, is a transcriptional coregulator that is required for the maintenance of energy homeostasis and ovulation. Although several studies have identified roles for NRIP1 in various cell processes, the biological functions of NRIP1 in esophageal squamous cell carcinoma (ESCC) remain unknown. In the present study, we demonstrate that NRIP1 inhibited the migration and invasion of ESCC cells. Further mechanistic studies revealed that NRIP1 is directly targeted by miR-548-3p and miR-576-5p...
June 26, 2018: Neoplasma
N Li, K Jiang, L P Fang, L L Yao, Z Yu
Long noncoding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in gastric cancer (GC) tissues compared with normal counterparts and CCAT1 upregulation can promote proliferation and migration of GC cells in vitro. B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) expression is positively correlated with tumor progression. The present study aimed to investigate the biological functions of CCAT1 and the relationships between CCAT1 and Bmi-1 in GC progression. In the present study, CCAT1 was knocked down by specific shRNA transfection in two human GC cell lines (MGC-803 and SGC-7901)...
June 26, 2018: Neoplasma
S H Huang, T Zhang, C G Zhao, J Qin, P Qi, F T Li, X J He
The aim of the study was to investigate the anticancer potential of Aclidinium bromide (INN) in glioblastoma. Glioblastoma cell lines U251 and U87 were treated with INN. The effects of INN on cell migration and invasion were assessed by transwell migration and invasion assays. Additionally, the effects of INN on the proliferation and apoptosis were detected by CCK-8 kit and flow cytometry. Western blotting was employed to determine anti-apoptotic proteins and the signaling pathway changes. The results showed that INN effectively suppressed proliferation, migration and invasion and induced apoptosis in U251 and U87 cells, respectively...
June 26, 2018: Neoplasma
K Dinas, V Moschaki, K Grammanikou, L Zepiridis, G Pratilas, A Sotiriadis, M Kalder
The aim of this study was to assess breast self-examination (BSE) practice in a representative sample of Greek midwives and midwifery students. Breast self-examination (BSE) is infrequent in healthcare professionals, including physicians and nurses. All midwives (n = 245) and graduating midwifery students (n = 165) who attended a congress of midwives were eligible for participation in the study. A self-administered, anonymous questionnaire was developed to assess BSE practice. Midwives performed BSE more frequently than students (p ent: BSE education should be included in the curricula of midwifery schools in order to increase BSE frequency, improve BSE accuracy and promote BSE teaching to patients...
June 26, 2018: Neoplasma
P Q Liang, M Miao, R Hu, Z G Liu, H N Jiang, C Li, S Y Ma
The relationships between autophagy-associated gene expression and clinical characteristics and prognosis in acute myeloid leukemia (AML) have not been well revealed. We examined mRNA expression of Bcl-2, p62, Beclin 1, VPS34, Rubicon, ALFY, UVRAG, ULK1, LC3 and NBR1 in 20 AML cases and 10 benign hematological cases by real-time PCR. Clinical information, treatment responses and outcomes of the AML patients were collected. Beclin 1, LC3, UVRAG, Rubicon and NBR1 were downregulated in AML patients compared with control group (P Keywords: acute myeloid leukemia; autophagy; clinical outcome...
June 26, 2018: Neoplasma
F Guo, D Jiao, G Q Sui, L N Sun, Y J Gao, Q F Fu, C X Jin
YWHAZ (14-3-3ζ) has been reported to be a prognostic marker for various tumors and play a crucial role in many oncogenic processes, including proliferation, migration and invasion. However, the functional role and mechanism of YWHAZ in gastric cancer (GC) are not in detail and still remain to be studied. In the present study, the endogenous expression of YWHAZ in gastric cancer cell line BGC-823 was silenced by YWHAZ-specific short hairpin RNA (shRNA). Our data showed that YWHAZ silencing resulted in cell cycle arrest in BGC-823 cells...
June 26, 2018: Neoplasma
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