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Priyanka Sharma, Rinu Sharma
Esophageal cancer (EC) is a highly complex disease with high incidence and mortality rates. Recent studies have shown that miRNAs play critical roles in diverse biological processes including oncogenesis, and we previously reported significantly increased expression of tissue and circulating miR-144 in EC. This study evaluates the functional significance of miR-144 in esophageal squamous cell carcinoma. Herein, we analysed the role of miR-144 in ESCC by silencing it in KYSE-410 cells, and followed this with cell cycle analysis and the following assays; MTT, annexin, colony formation, scratch and matrigel invasion assay...
June 26, 2018: Neoplasma
L Wachsmannova, J Majek, V Zajac, V Stevurkova, S Ciernikova
The development of colorectal cancer is affected by many factors, especially the intestinal microbiota. However, precise knowledge of bacterial communities associated with the mucosa in various parts of the colon is limited. Herein, we applied the gentamicin protection assay and detected the presence of intracellular bacteria in colorectal biopsies from Slovak patients with colorectal adenoma and carcinoma, and we compared this with healthy controls. The ENTEROtest 24 and MALDI-TOF mass spectrometry identified the cultivated bacteria and results revealed the presence of intracellularly localized Escherichia coli, Proteus mirabilis and Proteus vulgaris in patients with colorectal adenomas and carcinomas...
June 26, 2018: Neoplasma
J M Zhao, W Cheng, X G He, Y L Liu, F F Wang, Y F Gao
Lung cancer remains the most common cause of tumor-related death worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in the development of various cancers, including lung cancer. This study aimed to investigate the effect and the molecular basis of lncRNA PICART1 on lung cancer. We first assessed the PICART1 expression in lung cancer in vitro and vivo by qRT-PCR. Then the expression of PICART1 in SPC-A-1 and NCI-H1975 cell lines was inhibited and overexpressed by transient transfections...
June 26, 2018: Neoplasma
L Tian, Z Z Tao, H P Ye, G Y Li, Z F Zhan, H W Tuo
The early-stage diagnosis and treatment for the recurrence of larynx carcinoma needs further investigation. Mesenchyme homeobox 2 (MEOX2) was speculated as a novel suppressor gene in larynx carcinoma in our study, the molecular mechanism was studied. Real-time quantitative PCR (RT-qPCR) and Western blot were used to detect mRNA and protein levels of MEOX2 in laryngeal cancer tissues and cells (Hep-2, TU212, AMC-NH-8 and TU686 cells), and also apoptosis and phosphoinositide 3-kinase (PI3K)/protein kinase (Akt) related factors in TU212 cells transfected with MEOX2...
June 26, 2018: Neoplasma
R Sobotka, O Capoun, T Hanus, T Zima, M Kalousova, V Soukup
To assess the prognostic importance of serum levels of osteopontin (OP), stanniocalcin-1 (SC), FGF-23, alpha Klotho and 25-OH-D at the time of diagnosis in renal cell carcinoma (RCC) patients a total of 56 RCC patients with staging ≥ pT1b were enrolled in a prospective study. The relationship between the serum level of the analysed parameters and recurrence-free survival (RFS), overall survival (OS) and cancer-specific survival (CSS) was examined. Control group consisted of 20 patients without cancer. Level of osteopontin, stanniocalcin-1, FGF-23 and alpha Klotho were determined by Enzyme-Linked Immunosorbent Assay (ELISA), 25-OH-D by chemiluminiscence immunoanalysis (CLIA)...
June 26, 2018: Neoplasma
X M Lu, H Long
Cancer is the main leading cause of death in the world, although it has been made noteworthy advances in cancer research in the past decades. Early detection of cancer is extremely important in improving the chances of successful therapy. Thus, it is urgently needed to make further efforts to explore novel tumor markers for treatment.Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme which catalyzes the N-methylation of nicotinamide to form 1-methylnicotinamide (1-MNA), and plays an important role in controlling the intracellular concentration of nicotinamide...
June 26, 2018: Neoplasma
S K Chang, J Hou, G G Chen, D D Yu, H Q Wu, Y S Xie, L N Hu, L Gao, W Q Xiao, Y Y Kong, J M Shi
Natural killer (NK) cell-based immunotherapy is promising, as NK cells are in the first line of defense against cancer and capital of lysing tumor cells without pre-stimulation. However, NK cells from multiple myeloma (MM) patients are always deficient in numbers and the expression of certain activating receptors, disabling them in cytotoxicity against the cancer. Therefore, effective strategies to expand NK cells and increase NK cell-mediated cytotoxicity against MM are significant. Here, NK cells were efficiently expanded from peripheral blood mononuclear cells (PBMCs) of newly diagnosed MM patients after co-culture with irradiated K562 cells transfected with 41BBL and membrane-bound interleukin (IL)-15 (K562-mb15-41BBL) in the presence of 200 IU/ml human IL-2...
June 26, 2018: Neoplasma
K Hasakova, M Vician, R Reis, M Zeman, I Herichova
Colorectal cancer (CRC) exhibits differences in its features depending on the location of the tumor. The role of the circadian system in the carcinogenesis is accepted and many studies report different clock gene expression in tumors compared to healthy tissue, although a little attention is given to the changes in clock genes in tumors arising from various locations across the colon and rectum. The aim of our study was to investigate the expression of the clock genes cry1 and cry2 in human CRC tissue and tissue adjacent to colorectal tumors in a cohort of 64 patients by real time PCR...
June 26, 2018: Neoplasma
K Vicikova, E Petrovcikova, P Manka, J Drach, H Stockinger, V Leksa
The mannose 6-phosphate/insulin-like growth factor 2 receptor (CD222, M6P/IGF2R) is a multifunctional transmembrane type I receptor, mostly localized intracellularly, less on the surface of all types of mammalian cells. It is known both to transport lysosomal enzymes through their mannose 6-phosphate moieties and to internalize extracellular ligands like insulin-like growth factor 2 or plasminogen. CD222 is involved in regulation of cell proliferation, migration, T cell activation, and apoptosis. Soluble CD222 has been found in higher concentrations in sera of liver disease patients...
June 26, 2018: Neoplasma
S Q Feng, X Y Zhang, H T Fan, Q J Sun, M Zhang
It was proved that maternally expressed 3 (MEG3), a long non-coding RNA (LncRNA), is down-regulated and inversely correlated with the prognosis in various types of cancer, including bladder cancer (BC). Nevertheless, the role of MEG3 in BC has not been fully elaborated. Here, we found that MEG3 expression was reduced in 21 BC tumor tissue samples compared with the corresponding adjacent tissues. Then, we established T24 and 5637 cells with a stably integrated expression of MEG3 by G418 resistance screening...
June 26, 2018: Neoplasma
G Helbig, A Armatys, K Boral, A J Kopinska, K Wozniczka, M Dworaczek, K Chromik, A Koclega, M Panz-Klapuch, M Wysocka, A Janikowska
The incorporation of pegylated asparaginase (PEG-ASP) into pediatric and adult acute lymphoblastic leukemia (ALL) protocols remains a worldwide therapeutic standard, however the safety profile is a challenge. We herein report, in adults, the toxicity of intravenous single dose of 1000 IU/m2 PEG-ASP administered in remission induction for ALL patients. Thirty two patients at median age at diagnosis of 32 years (median 19-65) were included in this analysis. Most patients had B-cell lymphoblastic leukemia (n=26; 78%) and 81% of cases were dministration to first toxic symptoms was 7 days (range 1-19)...
June 26, 2018: Neoplasma
J Wang, X Q Chu, D Zhang, D F Kong
Gastric cancer is the main leading cause of cancer-related death worldwide. The aberrant expression of paternally expressed gene 10 (PEG10) is involved in development of a range of cancers. However, the potential biological function and the underling mechanism of PEG10 in human gastric carcinoma are still unknown. Knocking down LncRNA PEG10 might represent a promising therapeutic strategy for the treatment of gastric cancer. The expression of PEG10, miR-3200, and AEG1 in human gastric carcinoma NCI-N87 cells were altered by cell transfection assay...
June 26, 2018: Neoplasma
W J Wu, H Yin, J J Hu, X Z Wei
Mounting evidence indicates that long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. LncRNA LINC00313 has been found to be upregulated and associated with the poor prognosis of lung cancer. However, the potential role and clinical value of LINC00313 in human papillary thyroid cancer (PTC) remain elusive and need to be examined. The aim of the present study was to investigate the role of LINC00313 in PTC. We found that the expression of LINC00313 was significantly upregulated in PTC tissues and cell lines and that this upregulation was correlated with a poor prognosis...
June 26, 2018: Neoplasma
R Vrba, D Vrana, C Neoral, R Aujesky, M Stasek, T Bohanes, J Tesarikova, J Cincibuch, B Melichar, M Horakova, J Zapletalova
A single-center retrospective study the complication and mortality of surgical treatment of esophageal cancer 2006 to 2015 is presented. A total of 212 patients with esophageal cancer were operated at the First Department of Surgery University Hospital Olomouc, Czech Republic in the period between 2006 and 2015. Adenocarcinoma was histologically described in 127 patients (59.9%), squamous cell carcinoma in 82 patients (38.7%), and other types of carcinoma were described in 3 cases. According to the preoperative staging of esophageal cancer, the patients with early stage disease (T1-2N0M0) had primary surgery, while the patients with advanced stage (T3-4N0-2M0) were indicated for neoadjuvant chemoradiation with the surgery being performed subsequently...
June 26, 2018: Neoplasma
R Meng, J Fang, Y Yu, L K Hou, J R Chi, A X Chen, Y Zhao, X C Cao
Dysregulation of microRNA (miRNA) is closely related to cancer development and progression. Our previous study has been identified that miR-129-5p suppresses the proliferation and metastasis in breast cancer cells. In this study, we aimed to explore the target gene of miR-129-5p. We found that CBX4, a member of CBXs, is a target of miR-129-5p. CBX4 is up-regulated in breast cancer tissues and overexpression of CBX4 promotes cell proliferation, whereas knockdown of CBX4 suppresses cell proliferation in breast cancer cells...
June 26, 2018: Neoplasma
M Unseld, M Filip, S Seirl, A Gleiss, D Bianconi, M Kieler, S Demyanets, W Scheithauer, C Zielinski, G Prager
The oral multikinase inhibitor regorafenib had beneficial effects in randomized clinical phase III trials compared to the placebo in patients with metastatic colorectal cancer (mCRC) who progressed on standard therapies. The factors which influence regorafenib response and therapy sequence during treatment history are still highly discussed, and herein we analyzed the therapy algorithm, outcome and clinical markers following regorafenib application in a single center register study.Clinical data for 48 metastatic colorectal cancer patients were collected from 01...
June 26, 2018: Neoplasma
K Jasek, I Kasubova, V Holubekova, A Stanclova, L Plank, Z Lasabova
Many diseases have different pathological backgrounds responsible for abnormal cell behavior and exhibiting altered function and signal transduction. This is especially true for tumors and although changes affecting DNA sequence, irreversible mutations and chromosomal aberrations in gastrointestinal stromal tumors (GISTs) have been widely studied, the importance of reversible epigenetic changes increasingly recognized in many cancers has received insufficient attention in these tumors. Epigenetic mechanisms are part of normal development and gene expression under normal conditions, but malfunction of these processes leads to malignant transformation by disturbing both intra- and intercellular communication...
June 26, 2018: Neoplasma
C X Zeng, L Y Tang, C Y Xie, F X Li, J Y Zhao, N Jiang, Z Tong, S B Fu, F J Wen, W S Feng
The homology of epidermal growth factor receptor pathway substrate 8 (EPS8), EPS8L3, is elevated significantly in hepatocellular carcinoma (HCC) tissues and cell lines compared with the normal liver tissues and cell lines. The MTT and colony formation assays demonstrated that overexpressing EPS8L3 enhances, while silencing reduces the proliferation of HCC cells. Further experiments illustrated that overexpressing EPS8L3 promotes the expression of p-AKT, Cyclin D1, but inhibits the transcriptional activity of FOXO1...
June 26, 2018: Neoplasma
J X Yang, Y Sun, L Gao, Q Meng, B Y Yang
Glioma is among the most fatal brain tumors characterized by a highly malignancy and rapid progression and early metastasis. Dysregulation of long non-coding RNA differentiation antagonizing non-protein coding RNA (LncRNA DANCR) is associated with the development, progression and metastasis of various cancers. In the present study, we investigated functional role of LncRNA DANCR in the malignancy of glioma. The results showed that LncRNA DANCR was increased in glioma tissues and cells compared with normal brain tissues and cells...
June 26, 2018: Neoplasma
M Stork, S Sevcikova, Z Adam, M Krejci, V Sandecka, Z Kral, L Brozova, R Velichova, L Pour
The combination of lenalidomide and dexamethasone is the current gold standard for treatment of relapsed multiple myeloma. This study analyzes the efficiency of repeated lenalidomide treatment in patients with relapsed and refractory multiple myeloma. A total of 41 patients were prospectively evaluated at the University Hospital Brno. Lenalidomide was administered at standard dosing and in combination with corticosteroids and/or chemotherapy. The maximum cumulative dose of lenalidomide was limited to 4,200mg because of Czech health insurance rules...
June 26, 2018: Neoplasma
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