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Folia Microbiologica

Bhagwan Rekadwad, Juan M Gonzalez
The full biotechnological exploitation of thermostable enzymes in industrial processes is necessary for their commercial interest and industrious value. The heat-tolerant and heat-resistant enzymes are a key for efficient and cost-effective translation of substrates into useful products for commercial applications. The thermophilic, hyperthermophilic, and microorganisms adapted to extreme temperatures (i.e., low-temperature lovers or psychrophiles) are a rich source of thermostable enzymes with broad-ranging thermal properties, which have structural and functional stability to underpin a variety of technologies...
November 1, 2018: Folia Microbiologica
Jana Fránová, Igor Koloniuk, Ondřej Lenz, Dimitriyka Sakalieva
A quarantine organism, "Candidatus Phytoplasma mali," is the causal agent of apple proliferation, one of the most important apple diseases in Europe. The genetic diversity of this pathogen in Central and Southern Europe has already been reported; however, almost no data exists from Eastern Europe. In this study, "Ca. P. mali" strains, which were identified in 14 apple trees from the Bulgarian germplasm collection, were characterized by restriction fragment length polymorphism (RFLP) and sequence analysis of four genomic loci...
October 30, 2018: Folia Microbiologica
Radim Dobias, Michal Filip, Katerina Vragova, Dagmar Dolinska, Petra Zavodna, Ales Dujka, Petr Linzer, Patrik Jurek, Barbora Studena, Eva Cerna, Jakub Mrazek, Pavla Jaworska, Michaela Kantorova, Pavlina Lyskova, Eva Krejci, Vit Hubka
Cerebral abscesses caused by dark-pigmented Fonsecaea fungi are rare, especially in otherwise healthy individuals. In this case report, we present a 61-year-old man from Moldova, living in the Czech Republic, who had worked as a locksmith on oil platforms in Turkmenistan, Kazakhstan, Sudan, and Iraq since 1999, and was admitted to a neurology ward for a sudden motion disorder of the right leg, dysarthria, and hypomimia. Imaging revealed presence of expansive focus around the left lateral ventricle of the brain and a pronounced peripheral edema...
October 27, 2018: Folia Microbiologica
Lucélia Santi, Caio J B Coutinho-Rodrigues, Markus Berger, Lisete A S Klein, Eduardo M De Souza, Rafael L Rosa, Jorge A Guimarães, John R Yates, Wendell M S Perinotto, Vânia R E P Bittencourt, Walter O Beys-da-Silva
Beauveria bassiana is widely studied as an alternative to chemical acaricides in controlling the cattle tick Rhipicephalus microplus. Although its biocontrol efficiency has been proved in laboratory and field scales, there is a need to a better understanding of host interaction process at molecular level related to biocontrol activity. In this work, applying a proteomic technique multidimensional protein identification technology (MudPIT), the differential secretome of B. bassiana induced by the host R. microplus cuticle was evaluated...
October 25, 2018: Folia Microbiologica
Shahid Mahmood Chohan, Naeem Rashid, Muhammad Sajed, Tadayuki Imanaka
The genome sequence of Pyrobaculum calidifontis contains two open reading frames, Pcal_0144 and Pcal_0970, exhibiting homology with L-asparaginases. In search of a thermostable L-asparaginase with no glutaminase activity, we have cloned and expressed the gene encoding Pcal_0970 in Escherichia coli. Recombinant Pcal_0970 was produced in insoluble and inactive form which was solubilized and refolded into enzymatically active form. The refolded Pcal_0970 showed the highest activity at or above 100 °C. Optimum pH for the enzyme activity was 6...
October 25, 2018: Folia Microbiologica
Ahmad Raza, Saira Bashir, Romana Tabassum
Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes. This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92. Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23 ) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase. The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h...
October 25, 2018: Folia Microbiologica
Yu V Zaitseva, V A Lipasova, V A Plyuta, O A Koksharova, I V Demidyuk, S V Kostrov, I A Khmel
The luxS gene is responsible for the synthesis of AI-2 (autoinducer-2), a signaling molecule that participates in quorum sensing regulation in a large number of bacteria. In this work, we investigated which phenotypes are regulated by luxS gene in Serratia proteamaculans 94, psychrotrophic strain isolated from spoiled refrigerated meat. AI-2 was identified in S. proteamaculans 94, and the luxS gene involved in its synthesis was cloned and sequenced. A mutant with the inactivated luxS gene was constructed. Inactivation of the luxS gene was shown to lead to the absence of AI-2 synthesis, chitinolytic activity, swimming motility, suppression of the growth of fungal plant pathogens Rhizoctonia solani and Helminthosporium sativum by volatile compounds emitted by S...
October 25, 2018: Folia Microbiologica
Marianna Małek, Paulina Mrowiec, Karolina Klesiewicz, Iwona Skiba-Kurek, Adrian Szczepański, Joanna Białecka, Iwona Żak, Bożena Bogusz, Jolanta Kędzierska, Alicja Budak, Elżbieta Karczewska
Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis. Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epidemiology of the Candida glabrata species complex is available. Due to life-threatening infections caused by these species, it is crucial to supplement this knowledge. The aim of the study was to estimate the prevalence of C...
October 25, 2018: Folia Microbiologica
Mahsa Yazdi, Majid Bouzari, Ezzat Allah Ghaemi
Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family...
October 4, 2018: Folia Microbiologica
Thais Chimati Felix, Denise Von Dolinger de Brito Röder, Reginaldo Dos Santos Pedroso
When it comes to women's health, treating vaginal infections makes up a high proportion of the gynecological services. Among the forms of vaginitis, vulvovaginal candidiasis (VVC) is considered the second most common. Demand for new treatment alternatives is increasingly relevant, especially for therapies with fewer side effects, better tolerability, and lower cost, while still offering improved quality of life in terms of disease prevention. This study intended to investigate the alternative therapies described for the adjuvant treatment of vulvovaginitis caused by Candida species, including alternative and complementary treatment methods used by women...
September 30, 2018: Folia Microbiologica
Abidemi Anota Sunmola, Omonike O Ogbole, Temitope O C Faleye, Adewale Adetoye, Johnson A Adeniji, Funmilola A Ayeni
Enteroviruses have been associated with a host of clinical presentations including acute flaccid paralysis (AFP). The site of primary replication for most enteroviruses is the gastrointestinal tract (GIT) and lactic acid bacteria (LAB) may confer protection in the GIT against them. This study therefore investigates the antiviral potential of some selected lactic acid bacteria against enterovirus isolates recovered from AFP cases. The antiviral activities of Lactobacillus plantarum, Lactobacillus amylovorus, and Enterococcus hirae in broth culture, their cell-free supernatant (CFS), and bacterial cell pellets were assayed against Echovirus 7 (E7), E13, and E19 in a pre- and post-treatment approach using cytopathic effect (CPE) and cell viability (MTT) assay...
September 29, 2018: Folia Microbiologica
Xênia M Souto, Lívia S Ramos, Marta H Branquinha, André L S Santos
The Candida haemulonii complex (Candida haemulonii, Candida haemulonii var. vulnera, and Candida duobushaemulonii) comprises emerging opportunistic human fungal pathogens with recognized multidrug-resistance profiles. Little is known about the virulence markers produced by this fungal complex. However, it is recognized that Candida spp. express a large array of peptidases, which play multiple roles in different aspects of fungal-host interactions. In the present study, we have identified proteolytic enzymes in clinical isolates of the C...
September 26, 2018: Folia Microbiologica
Sema Alacam, Nuran Karabulut, Ayfer Yolcu, Mustafa Onel, Alp Atasoy, Sabahattin Kaymakoglu, Ali Agacfidan
Mutations occurring in viral polymerase gene of hepatitis B virus (HBV) due to the use of nucleos(t)id analogs reduce the activity of the drugs by causing antiviral resistance. In this study, it was aimed to evaluate mutations responsible for drug resistance and drug resistance mutation rates in patients followed up by the diagnosis of chronic hepatitis B (CHB). A total of 318 CHB patients were included in the study. HBV mutations were detected using the INNO-LiPA commercial kit based on the reverse hybridization principle...
September 26, 2018: Folia Microbiologica
Lucia Mališová, Renáta Šafránková, Jana Kekláková, Petr Petráš, Helena Žemličková, Vladislav Jakubů
A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin...
September 20, 2018: Folia Microbiologica
Fuat Aydın, Vehbi Güneş, Latife Çakır Bayram, Seçil Abay, Emre Karakaya, Kemal Varol, Gencay Ekinci, Kadir Semih Gümüşsoy, Hamit Kaan Müştak, Kadir Serdar Diker
Here, we report a case of neonatal calf meningitis due to Streptococcus gallolyticus subsp. gallolyticus (SGG). Clinical, pathological and microbiological findings were evaluated. API Strep, 16S rRNA gene sequencing, rpoB gene sequencing and sodA gene sequencing were used for the complete identification of SGG. This is the first documented report of neonatal calf meningitis due to SGG in veterinary medicine.
September 19, 2018: Folia Microbiologica
Ana Luiza do Rosário Palma, Nádia Domingues, Patrícia Pimentel de Barros, Graziella Nuernberg Back Brito, Antônio Olavo Cardoso Jorge
The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of Candida albicans, Streptococcus mitis, and Streptococcus sanguinis and their in vivo pathogenicity. The in vitro analysis evaluated the effects of S. mitis and S. sanguinis on C. albicans's biofilm formation by CFU count, filamentation capacity, and adhesion (ALS1, ALS3, HWP1) and transcriptional regulatory gene (BCR1, CPH1, EFG1) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on Galleria mellonella, with analyses of the CFU per milliliter count and filamentation...
September 19, 2018: Folia Microbiologica
Maxim S Kharchuk, Andrey N Glushenkov, Elena N Gromozova
The moving volutin (polyphosphate) granules known as "dancing bodies" can be observed in the vacuoles of the yeast cells. The aim of work was to study the effects of cultivation conditions and influences of physico-chemical factors on the motion of vacuolar volutin granules in Saccharomyces cerevisiae cells. The motion of granules is a non-Markovian process. It does not depend on the cell cycle phase, but depends on the growth stage. The maximal number of cells with "dancing bodies" was observed under cultivation of yeast at 25-28 °C and pH 5...
September 11, 2018: Folia Microbiologica
Jimmy Kuo, Yen-Wen Wang, Michael Chen, Georgia Fuh, Chorng-Horng Lin
Paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4, 4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-ol, is a plant growth retardant that mainly inhibits gibberellins (GAs) biosynthesis. In agricultural practice, paclobutrazol is applied to arrest vegetative growth so as to increase the reproductive growth of many orchard fruit, as well as grain crops. However, due to its over-application and chemical stability, paclobutrazol accumulates in soil and inhibits the growth of subsequent crops, especially those grown for vegetative purposes...
August 28, 2018: Folia Microbiologica
Elham Atefi Tabar, Hamid Staji, Ali Mahdavi
Turkeys and broilers have been identified as important reservoirs for Campylobacter jejuni which is of public health significance. The evaluation of the genotypes among C. jejuni strains within different reservoirs is critical for our understanding of the epidemiology of this infectious agent. The present study aimed to compare the genetic diversity and differences of C. jejuni isolates from turkeys and broilers using flagellin PCR-RFLP typing (flaA typing) technique, in terms of the ease of use and discriminatory power...
August 27, 2018: Folia Microbiologica
Poulomi Chakraborty, Sutapa Joardar, Shounak Ray, Papu Biswas, Debasish Maiti, Prosun Tribedi
Microbial biofilms are factions of surface-colonized cells encompassed in a matrix of extracellular polymeric substances. Profound application of antibiotics in order to treat infections due to microbial biofilm has led to the emergence of several drug-resistant microbial strains. In this context, a novel type of 3,6-di(pyridin-2-yl)-1,2,4,5-tetrazine (pytz)-capped silver nanoparticles (TzAgNPs) was synthesized, and efforts were given to test its antimicrobial and antibiofilm activities against Pseudomonas aeruginosa, a widely used biofilm-forming pathogenic organism...
November 2018: Folia Microbiologica
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