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Folia Microbiologica

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https://www.readbyqxmd.com/read/28409422/influence-of-long-term-equine-herpesvirus-type-1-ehv-1-infection-on-primary-murine-neurons-the-possible-effects-of-the-multiple-passages-of-ehv-1-on-its-neurovirulence
#1
Joanna Cymerys, A Słońska, A Tucholska, A Golke, A Chmielewska, M W Bańbura
Equine herpesvirus 1 (EHV-1), like other members of the Alphaherpesvirinae subfamily, is a neurotropic virus causing latent infections in the nervous system of the natural host. In the present study, we have investigated EHV-1 replication (wild-type Jan-E strain and Rac-H laboratory strain) during long-term infection and during the passages of the virus in cultured neurons. The studies were performed on primary murine neurons, which are an excellent in vitro model for studying neurotropism and neurovirulence of EHV-1...
April 13, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28401403/analysis-of-antimicrobial-and-immunomodulatory-substances-produced-by-heterofermentative-lactobacillus-reuteri
#2
Gabriela Greifová, Hyacinta Májeková, Gabriel Greif, Patrik Body, Maria Greifová, Martina Dubničková
Antimicrobial and immunomodulatory potential of various Lactobacillus reuteri strains is closely connected to their metabolite production profile under given cultivation conditions. We determined the in vitro production of antimicrobial substances such as organic acids, ethanol, and reuterin by four strains of L. reuteri (L. reuteri E, L. reuteri KO5, L. reuteri CCM 3625, and L. reuteri ATCC 55730). All studied L. reuteri strains showed the ability to produce lactic acid, acetic acid, and ethanol with concominant consumption of glucose and together with phenyllactic acid-a potent antifungal compound-with concominant consumption of phenylalanine...
April 11, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28382524/characterization-of-a-metagenome-derived-protease-from-contaminated-agricultural-soil-microorganisms-and-its-random-mutagenesis
#3
Chengjian Jiang, Liang Zhang, Fajia Li, Can Meng, Rong Zeng, Jie Deng, Peihong Shen, Qian Ou, Bo Wu
Proteases are typical key enzymes that hydrolyze proteins into amino acids and peptides. Numerous proteases have been studied, but the discovery of metagenome-derived proteases is still significant for both commercial applications and basic research. An unexplored protease gene sep1A was identified by function-based screening from a plasmid metagenomic library derived from uncultured contaminated agricultural soil microorganisms. The putative protease gene was subcloned into pET-32a (+) vector and overexpressed in E...
April 5, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28365878/biofilm-formation-antibiotic-susceptibility-and-rapd-genotypes-in-pseudomonas-aeruginosa-clinical-strains-isolated-from-single-centre-intensive-care-unit-patients
#4
Martina Vaněrková, Barbora Mališová, Iva Kotásková, Veronika Holá, Filip Růžička, Tomáš Freiberger
The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed...
April 1, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28364392/burkholderia-cepacia-complex-in-cystic-fibrosis-in-the-post-epidemic-period-multilocus-sequence-typing-based-approach
#5
Libor Fila, Pavel Dřevínek
Cystic fibrosis (CF) patients in the Czech Republic suffered in the late 1990s from an epidemic with ST32 strain of Burkholderia cepacia complex (Bcc). Cohort segregation of Bcc and of ST32 positive patients was introduced in 1999 and 2002, respectively. We performed a study to evaluate the molecular epidemiology of Bcc infection after implementation of these infection control measures. Patients attending the Prague CF adult Centre from 2000 to 2015 were included in the present study. Demographic data and microbial statuses were collected from patient records...
March 31, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28361459/distribution-and-antifungal-susceptibility-of-yeasts-isolates-from-intensive-care-unit-patients
#6
Vladimír Hrabovský, Victoria Takáčová, Eva Schréterová, Lydia Pastvová, Zuzana Hrabovská, Katarina Čurová, Leonard Siegfried
Yeasts frequently colonize non-sterile sites in the body. The aim of the study was to determine distribution in clinical samples and antifungal susceptibility to five antifungals. From January 2013 through June 2015, 800 isolates were obtained from intensive care unit patients. Candida albicans (58.9%), Candida glabrata (20.4%), Candida krusei (8.6%), and Candida parapsilosis (3.6%) were the leading species. Majority of the C. albicans isolates were susceptible to the fluconazole. Elevated voriconazole minimal inhibitory concentrations (MICs) were observed in isolates exhibiting high fluconazole MICs, most frequently in C...
March 30, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28353080/serological-culture-and-molecular-survey-of-mycobacterium-avium-paratuberculosis-in-a-goat-flock-in-tuscany
#7
Alessia Galiero, Barbara Turchi, Francesca Pedonese, Roberta Nuvoloni, Carlo Cantile, Giuseppe Colombani, Mario Forzan, Domenico Cerri, Patrizia Bandecchi, Filippo Fratini
Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagnana district (Tuscany, Italy) performing ELISA tests, culture and IS900 PCR assay; direct diagnostic methods were carried out not only on bulk tank milk and cheese samples but also on individual milk and tissue specimens collected from nine subjects positive to ELISA tests...
March 28, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28349416/the-effect-of-the-colostral-cells-on-gene-expression-of-cytokines-in-cord-blood-cells
#8
Jiří Hrdý, Olga Novotná, Ingrid Kocourková, Ludmila Prokešová
Beneficial effect of maternal milk is acknowledged, but there is still question whether maternal milk from allergic mother is as good as from healthy one. In our study, we have assayed the effect of cells from colostrum of healthy and allergic mothers on gene expression of cytokines in cord blood cells of newborns of healthy and allergic mothers. Cytokines typical for Th1 (IL-2, IFN-gamma), Th2 (IL-4, IL-13), Tregs (IL-10, TGF-beta), and IL-8 were followed. We were not able to detect significant influence of colostral cells on gene expression of cytokines in cord blood after 2-day coculture using Transwell system...
March 28, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28342148/further-characterization-and-independent-validation-of-a-dna-aptamer-quantum-dot-based-magnetic-sandwich-assay-for-campylobacter
#9
John G Bruno, Jeffrey C Sivils
Previously reported DNA aptamers developed against surface proteins extracted from Campylobacter jejuni were further characterized by aptamer-based Western blotting and shown to bind epitopes on proteins weighing ~16 and 60 kD from reduced C. jejuni and Campylobacter coli lysates. Proteins of these approximate weights have also been identified in traditional antibody-based Western blots of Campylobacter spp. Specificity of the capture and reporter aptamers from the previous report was further validated by aptamer-based ELISA-like (ELASA) colorimetric microplate assay...
March 24, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28337589/colonization-by-non-pathogenic-bacteria-alters-mrna-expression-of-cytochromes-p450-in-originally-germ-free-mice
#10
L Jourová, P Anzenbacher, B Lišková, Z Matušková, P Hermanová, T Hudcovic, H Kozáková, L Hrnčířová, E Anzenbacherová
Gut microbiota provides a wide range of beneficial function for the host and has an immense effect on the host's health state. It has also been shown that gut microbiome is often involved in the biotransformation of xenobiotics; however, the molecular mechanisms of the interaction between the gut bacteria and the metabolism of drugs by the host are still unclear. To investigate the effect of microbial colonization on messenger RNA (mRNA) expression of liver cytochromes P450 (CYPs), the main drug-metabolizing enzymes, we used germ-free (GF) mice, lacking the intestinal flora and mice monocolonized by non-pathogenic bacteria Lactobacillus plantarum (NIZO2877) or probiotic bacteria Escherichia coli Nissle 1917 compared to specific pathogen-free (SPF) mice...
March 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28332133/molecular-typing-and-antimicrobial-susceptibility-testing-to-six-antimicrobials-of-clostridium-difficile-isolates-from-three-czech-hospitals-in-eastern-bohemia-in-2011-2012
#11
V Beran, E J Kuijper, C Harmanus, I M Sanders, S M van Dorp, C W Knetsch, J Janeckova, A Seidelova, L Barekova, J Tvrdik, D Chmelar, I Ciznar
In 2011-2012, a survey was performed in three regional hospitals in the Czech Republic to determine the incidence of Clostridium difficile infections (CDIs) and to characterize bacterial isolates. C. difficile isolates were characterized by PCR ribotyping, toxin genes detection, multiple-locus variable-number tandem-repeat analysis (MLVA), and antimicrobial susceptibility testing to fidaxomicin, vancomycin, metronidazole, clindamycin, LFF571, and moxifloxacin using agar dilution method. The incidence of CDI in three studied hospitals was 145, 146, and 24 cases per 100,000 inhabitants in 2011 and 177, 258, and 67 cases per 100,000 inhabitants in 2012...
March 22, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28316009/safety-assessment-of-commensal-enterococci-from-dogs
#12
Ivana Kubašová, Viola Strompfová, Andrea Lauková
Enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. Generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. In these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. Because there is a lack of information on incidence of these attributes in animal commensal enterococci, we screened 160 strains originating from feces of clinically healthy dogs in Eastern Slovakia (n = 105)...
March 18, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28315021/clinical-strains-of-streptococcus-agalactiae-carry-two-different-variants-of-pathogenicity-island-xii
#13
Eugenia Kuleshevich, Joseph Ferretti, Ilda Santos Sanches, Natesan Balasubramanian, Barbara Spellerberg, Androulla Efstratiou, Paula Kriz, Kornelia Grabovskaya, Olga Arjanova, Alevtina Savitcheva, Valentin Shevchenko, Anton Rysev, Alexander Suvorov
Streptococcus agalactiae or Group B streptococci (GBS) are a common cause of serious diseases of newborns and adults. GBS pathogenicity largely depends on genes located on the accessory genome including several pathogenicity islands (PAI). The present paper is focused on the structure and molecular epidemiological analysis of one of the GBS pathogenicity islands-the pathogenicity island PAI XII (Glaser et al. Mol Microbiol 45(6):1499-1513, 2002). This PAI was found to be composed of three different mobile genetic elements: a composite transposon (PAI-C), a genomic islet (PAI-B), and a pathogenicity island associated with gene sspB1 (PAI-A)...
March 18, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28285373/rhizobia-a-potential-biocontrol-agent-for-soilborne-fungal-pathogens
#14
REVIEW
Krishnashis Das, Radha Prasanna, Anil Kumar Saxena
Rhizobia are a group of organisms that are well known for their ability to colonize root surfaces and form symbiotic associations with legume plants. They not only play a major role in biological nitrogen fixation but also improve plant growth and reduce disease incidence in various crops. Rhizobia are known to control the growth of many soilborne plant pathogenic fungi belonging to different genera like Fusarium, Rhizoctonia, Sclerotium, and Macrophomina. Antagonistic activity of rhizobia is mainly attributed to production of antibiotics, hydrocyanic acid (HCN), mycolytic enzymes, and siderophore under iron limiting conditions...
March 12, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28283946/association-between-caga-vacai-and-dupa-genes-of-helicobacter-pylori-and-gastroduodenal-pathologies-in-chilean-patients
#15
Esteban Paredes-Osses, Katia Sáez, Enrique Sanhueza, Sonja Hebel, Carlos González, Carlos Briceño, Apolinaria García Cancino
In addition to the already known cagA gene, novel genetic markers have been associated with Helicobacter pylori (H. pylori) virulence: the dupA and vacAi genes. These genes might play an important role as specific markers to determine the clinical outcome of the disease, especially the vacAi gene, which has been expected to be a good marker of severe pathologies like gastric adenocarcinoma. In the present study, the association of cagA, dupA, and vacAi genes with gastroduodenal pathologies in Chilean patients was studied...
March 11, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28283945/agar-degrading-bacteria-isolated-from-antarctic-macroalgae
#16
Roxana Alvarado, Sergio Leiva
This study describes the taxonomic diversity of pigmented, agar-degrading bacteria isolated from the surface of macroalgae collected in King George Island, Antarctica. A total of 30 pigmented, agarolytic bacteria were isolated from the surface of the Antarctic macroalgae Adenocystis utricularis, Monostroma hariotii, Iridaea cordata, and Pantoneura plocamioides. Based on the 16S rRNA data, the agarolytic isolates were affiliated to the genera Algibacter, Arthrobacter, Brachybacterium, Cellulophaga, Citricoccus, Labedella, Microbacterium, Micrococcus, Salinibacterium, Sanguibacter, and Zobellia...
March 10, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28281229/potential-of-pichia-pastoris-for-the-production-of-industrial-penicillin-g-acylase
#17
Helena Marešová, Andrea Palyzová, Martina Plačková, Michal Grulich, Vyasa Williams Rajasekar, Václav Štěpánek, Eva Kyslíková, Pavel Kyslík
This study deals with the potential of Pichia pastoris X-33 for the production of penicillin G acylase (PGA(A)) from Achromobacter sp. CCM 4824. Synthetic gene matching the codon usage of P. pastoris was designed for intracellular and secretion-based production strategies and cloned into vectors pPICZ and pPICZα under the control of AOX1 promoter. The simple method was developed to screen Pichia transformants with the intracellularly produced enzyme. The positive correlation between acylase production and pga gene dosage for both expression systems was demonstrated in small scale experiments...
March 9, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28275945/enrichment-culture-and-identification-of-endophytic-methanotrophs-isolated-from-peatland-plants
#18
Zofia Stępniewska, Weronika Goraj, Agnieszka Kuźniar, Natalia Łopacka, Magdalena Małysza
Aerobic methane-oxidizing bacteria (MOB) are an environmentally significant group of microorganisms due to their role in the global carbon cycle. Research conducted over the past few decades has increased the interest in discovering novel genera of methane-degrading bacteria, which efficiently utilize methane and decrease the global warming effect. Moreover, methanotrophs have more promising applications in environmental bioengineering, biotechnology, and pharmacy. The investigations were undertaken to recognize the variety of endophytic methanotrophic bacteria associated with Carex nigra, Vaccinium oxycoccus, and Eriophorum vaginatum originating from Moszne peatland (East Poland)...
March 9, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28238067/acquisition-of-tn6018-3-cs-regions-increases-colistin-mics-against-acinetobacter-baumannii-isolates-harboring-new-variants-of-abars
#19
Mohammad Savari, Alireza Ekrami, Saeed Shoja, Abbas Bahador
Colistin is the last hope to treat extensively drug resistance (XDR) Acinetobacter baumannii (A. baumannii) infections, but resistance to colistin is currently reported in clinical centers all over the world. Here, we studied two colistin-resistant A. baumannii isolates with a difference in minimum inhibitory concentrations (MICs) that were isolated from a single burn patient during treatment in the hospitalization period. The international clonal (IC) lineage, multilocus sequence typing (MLST), and multiple loci variable number tandem repeat (VNTR) analysis (MLVA) typing were used to characterize the relatedness of A...
February 25, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28236177/virulence-genes-and-antimicrobial-susceptibility-of-lactose-negative-and-lactose-positive-strains-of-escherichia-coli-isolated-from-pregnant-women-and-neonates
#20
Agnieszka Kaczmarek, Krzysztof Skowron, Anna Budzyńska, Katarzyna Grudlewska, Eugenia Gospodarek-Komkowska
Escherichia coli can cause serious infections in the neonates and pregnant women. Although E. coli is widely studied, E. coli lactose-negative (lac-) strains have been rarely described before. So, the aim of this study was to compare lac- and lactose-positive (lac+) E. coli strains in respect of antimicrobial susceptibility and the frequency of virulence genes (VGs). The study included 58 lac+ and 58 lac- E. coli strains isolated from pregnant women and neonates. Culture and the results of biochemical reactions were conducted for lac- and lac+ E...
February 24, 2017: Folia Microbiologica
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