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Folia Microbiologica

Fuat Aydın, Vehbi Güneş, Latife Çakır Bayram, Seçil Abay, Emre Karakaya, Kemal Varol, Gencay Ekinci, Kadir Semih Gümüşsoy, Hamit Kaan Müştak, Kadir Serdar Diker
Here, we report a case of neonatal calf meningitis due to Streptococcus gallolyticus subsp. gallolyticus (SGG). Clinical, pathological and microbiological findings were evaluated. API Strep, 16S rRNA gene sequencing, rpoB gene sequencing and sodA gene sequencing were used for the complete identification of SGG. This is the first documented report of neonatal calf meningitis due to SGG in veterinary medicine.
September 19, 2018: Folia Microbiologica
Ana Luiza do Rosário Palma, Nádia Domingues, Patrícia Pimentel de Barros, Graziella Nuernberg Back Brito, Antônio Olavo Cardoso Jorge
The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of Candida albicans, Streptococcus mitis, and Streptococcus sanguinis and their in vivo pathogenicity. The in vitro analysis evaluated the effects of S. mitis and S. sanguinis on C. albicans's biofilm formation by CFU count, filamentation capacity, and adhesion (ALS1, ALS3, HWP1) and transcriptional regulatory gene (BCR1, CPH1, EFG1) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on Galleria mellonella, with analyses of the CFU per milliliter count and filamentation...
September 19, 2018: Folia Microbiologica
Maxim S Kharchuk, Andrey N Glushenkov, Elena N Gromozova
The moving volutin (polyphosphate) granules known as "dancing bodies" can be observed in the vacuoles of the yeast cells. The aim of work was to study the effects of cultivation conditions and influences of physico-chemical factors on the motion of vacuolar volutin granules in Saccharomyces cerevisiae cells. The motion of granules is a non-Markovian process. It does not depend on the cell cycle phase, but depends on the growth stage. The maximal number of cells with "dancing bodies" was observed under cultivation of yeast at 25-28 °C and pH 5...
September 11, 2018: Folia Microbiologica
Jimmy Kuo, Yen-Wen Wang, Michael Chen, Georgia Fuh, Chorng-Horng Lin
Paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4, 4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-ol, is a plant growth retardant that mainly inhibits gibberellins (GAs) biosynthesis. In agricultural practice, paclobutrazol is applied to arrest vegetative growth so as to increase the reproductive growth of many orchard fruit, as well as grain crops. However, due to its over-application and chemical stability, paclobutrazol accumulates in soil and inhibits the growth of subsequent crops, especially those grown for vegetative purposes...
August 28, 2018: Folia Microbiologica
Elham Atefi Tabar, Hamid Staji, Ali Mahdavi
Turkeys and broilers have been identified as important reservoirs for Campylobacter jejuni which is of public health significance. The evaluation of the genotypes among C. jejuni strains within different reservoirs is critical for our understanding of the epidemiology of this infectious agent. The present study aimed to compare the genetic diversity and differences of C. jejuni isolates from turkeys and broilers using flagellin PCR-RFLP typing (flaA typing) technique, in terms of the ease of use and discriminatory power...
August 27, 2018: Folia Microbiologica
Ivana Kubašová, Andrea Lauková, Ľudmila Hamarová, Peter Pristaš, Viola Strompfová
Some strains of the genus Enterococcus are effective probiotic bacteria if they meet safety and probiotic criteria. In our study, 17 canine enterococci previously selected from a group of 160 isolates based on safety criteria were screened for some functional properties relevant to their use as probiotics. The results of antimicrobial resistance testing showed sensitivity of eleven strains to EFSA recommended antimicrobials. In contrast, the most frequent resistance was observed for cefotaxim (15/17) and oxacillin (13/17)...
August 17, 2018: Folia Microbiologica
Xindong Xu, Li Han, Lixing Zhao, Xiao Chen, Cuiping Miao, Linfang Hu, Xueshi Huang, Youwei Chen, Yiqing Li
Actinomycete strain YIM PH20520, isolated from the rhizosphere soil sample of Panax notoginseng collected in Wenshang, Yunnan Province, China, exhibited antifungal activity against root-rot pathogens of the Panax notoginseng. The structures of bioactive molecules, isolated from the ethyl acetate extract of the fermentation broth of the strain, were identified as echinosporin (1) and 7-deoxyechinosporin (2) based on extensive spectroscopic analyses. 1 exhibited antifungal activity against four tested root-rot pathogens of Panax notoginseng include Fusarium oxysporum, Fusarium solani, Alternaria panax, and Phoma herbarum with the MIC value at 64, 64, 32, and 64 μg/mL, respectively...
August 16, 2018: Folia Microbiologica
Mohammed Baqur S Al-Shuhaib, Hawraa N Al-Kaaby, Sabah L Alwan
Neoscytalidium (or N.) dimidiatum and N. novaehollandiae are two aggressive plant pathogenic species that affect several agricultural crops. Early detection and identification of these fungi are of critical importance to bring about the effective minimization to the threat they pose to the infected plants. Herein, two species of Neoscytalidium were rapidly discriminated by utilizing the rRNA internal transcribed (ITS4-5.8S-ITS5) PCR primers. A total of 100 isolates of Neoscytalidium species, which were isolated from Iraqi canker-infected fig trees, were included in this study...
August 14, 2018: Folia Microbiologica
Branislav Lilić, Brankica Filipić, Milka Malešević, Katarina Novović, Zorica Vasiljević, Milan Kojić, Branko Jovčić
The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis...
August 13, 2018: Folia Microbiologica
Nastaran Farzi, Catherine Behzad, Zahra Hasani, Masoud Alebouyeh, Homayoun Zojaji, Mohammad Reza Zali
Mixed infections and heteroresistance of Helicobacter pylori contribute to decreased efficacy of treatments. This study aimed to investigate frequency of clarithromycin heteroresistance and its link with mixed infections, medication history, and disease severity. A total of 40 pairs of H. pylori strains were isolated from the antrum and corpus of 97 patients. Susceptibility of the strains to clarithromycin was measured by agar dilution method. Site-specific mutations of 23S rRNA at A2143G, A2142G, and A2142C positions were analyzed by PCR and genomic relatedness of pairs of the strains was determined by random amplified polymorphic DNA (RAPD)-PCR...
August 10, 2018: Folia Microbiologica
Chahrazed Mekadim, Jiří Killer, Radko Pechar, Jakub Mrázek
The order Lactobacillales represents a morphologically, metabolically, and physiologically diverse group of bacteria. Lactic acid bacteria represent the core of this phylogenetic group. They are a part of epiphytic microflora, fermented dairy, meat, fruit and vegetable products, and the digestive tract of humans and animals. Despite the fact that these bacteria form a phenotypically and genotypically heterogeneous group, their phylogenetic relationship enables to propose a common genetic marker usable in classification, typing, and phylogeny...
August 9, 2018: Folia Microbiologica
Muhsin Jamal, Saadia Andleeb, Fazal Jalil, Muhammad Imran, Muhammad Asif Nawaz, Tahir Hussain, Muhammad Ali, Sadeeq Ur Rahman, Chythanya Rajanna Das
Biofilm is involved in a variety of infections, playing a critical role in the chronicity of infections. Enterobacter cloacae is a biofilm-forming and multi-drug-resistant (MDR) nosocomial pathogen leading to significant morbidity and mortality. This study aimed at isolation of a bacteriophage against MDR clinical strain of E. cloacae and its efficacy against bacterial planktonic cells and biofilm. A bacteriophage MJ2 was successfully isolated from wastewater and was characterized. The phage exhibited a wide range of thermal and pH stability and demonstrated considerable adsorption to host bacteria in the presence of CaCl2 or MgCl2 ...
August 8, 2018: Folia Microbiologica
Rosario Díaz Rodríguez, Gabriela Heredia, José A Siles, Miguel Jurado, Mario Carlos Nazareno Saparrat, Inmaculada García-Romera, Inmaculada Sampedro
To obtain enzymatic preparations with higher laccase activity levels from Funalia floccosa LPSC 232, available for use in several applications, co-cultures with six filamentous microfungi were tested. A laccase non-producing soil fungus, identified as Penicillium commune GHAIE86, showed an outstanding ability to increase laccase activity (3-fold as compared to that for monoculture) when inoculated in 6-day-old F. floccosa cultures. Maximum laccase production with the F. floccosa and P. commune co-culture reached 60 U/mL, or twice that induced by chemical treatments alone...
August 6, 2018: Folia Microbiologica
Jana Kisková, Zuzana Stramová, Peter Javorský, Jana Sedláková-Kaduková, Peter Pristaš
Brown mud, as a waste product of the industrial process of aluminum production, represents a great environmental burden due to its toxicity to living organisms. However, some microorganisms are able to survive in this habitat, and they can be used in bioremediation processes. Traditional cultivation methods have a limited capacity to characterize bacterial composition in environmental samples. Recently, next-generation sequencing methods have provided new perspectives on microbial community studies. The aim of this study was to analyze the bacterial community in the drainage water of brown mud disposal site near Žiar nad Hronom (Banská Bystrica region, Slovakia) using 454 pyrosequencing...
August 6, 2018: Folia Microbiologica
Martina Paldrychová, Irena Kolouchová, Eva Vaňková, Olga Maťátková, Jan Šmidrkal, Aleš Krmela, Věra Schulzová, Jana Hajšlová, Jan Masák
Microorganisms that cause chronic infections exist predominantly as surface-attached stable communities known as biofilms. Microbial cells in biofilms are highly resistant to conventional antibiotics and other forms of antimicrobial treatment; therefore, modern medicine tries to develop new drugs that exhibit anti-biofilm activity. We investigated the influence of a plant polyphenolic compound resveratrol (representative of the stilbene family) on the opportunistic pathogen Trichosporon cutaneum. Besides the influence on the planktonic cells of T...
July 30, 2018: Folia Microbiologica
Pompeyo Ferro, Ivone Vaz-Moreira, Célia M Manaia
Members of the species Ralstonia pickettii and R. mannitolilytica, although ubiquitous and lacking major virulence factors, have been associated with nosocomial outbreaks. Tolerance to metals, antibiotics, and disinfectants may represent an advantage for their ubiquity and opportunistic pathogenic potential. In this study, we compared five strains that differed on the origin (hospital effluent, tap water, mineral water) and in the susceptibility to aminoglycosides, regarding their tolerance to metals and disinfection...
July 21, 2018: Folia Microbiologica
Maria-Cecília Krähenbühl Amstalden, José Diogo Oliveira, Mathias Strauss, Italo Odone Mazali, Daisy Machado, Thais Holtz Theisen, Marcelo Lancellotti
Streptococcus pneumoniae are natural competent bacteria which requires the presence of a pheromone-like molecule to do the transformation process. This study verified the influence of mesoporous silica (SBA-15 and SBA-16) on the transformation process in S. pneumoniae using a donor DNA obtained from a mutant strain of this microorganism (Sp360∆luxS). The results showed that mesoporous silica SBA-15 and SBA-16 particles doubled the transformation ratio frequency compared with negative control (without nanoparticles) in using SBA-15 (ratio 1...
July 14, 2018: Folia Microbiologica
Somayeh Kiaei, Mohammad Moradi, Hossein Hosseini Nave, Zahra Hashemizadeh, Majid Taati-Moghadam, Davood Kalantar-Neyestanaki
Dissemination of carbapenemase-producing Klebsiella pneumoniae along with 16S rRNA methyltransferase (16S-RMTase) has been caused as a great concern for healthcare settings. The aim of this study was to investigate the prevalence of resistance genes among K. pneumoniae isolates. During October 2015 to February 2016, 30 non-duplicative K. pneumoniae strains were isolated from clinical specimens in a burn center in Kerman, Iran. Antibiotic susceptibility tests of isolates, carbapenemase, extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamase-producing isolates were determined by phenotypic methods...
July 12, 2018: Folia Microbiologica
Martina Krehelova, Otakar Nyč, Eva Sinajová, Marcela Krutova
This study aimed to implement a toxigenic culture as an optional third diagnostic step for glutamate dehydrogenase (GDH)-positive and toxin A/B-negative diarrheal stool samples into a diagnostic algorithm for Clostridioides (Clostridium) difficile infection (CDI), and to characterise C. difficile isolates for epidemiological purposes. During the 5-month study, 481 diarrhoeal stool samples from three Slovak hospitals were investigated and 66 non-duplicated GDH-positive stool samples were found. Of them, 36 were also toxin A/B-positive...
July 3, 2018: Folia Microbiologica
Yanina A Delegan, Leonid N Valentovich, Samira M Shafieva, Khudaverdi G Ganbarov, Andrey E Filonov, Mikhail B Vainstein
A thermotolerant bacterial strain 1D isolated from refinery oil-contaminated soil was identified as Gordonia sp. based on the analysis of 16S rRNA and gyrB gene sequences. The strain was found to utilize crude oil, diesel fuel, and a wide spectrum of alkanes at temperatures up to 50 °C. Strain 1D is the first representative of Gordonia amicalis capable of utilizing alkanes of chain length up to С36 at a temperature of 45-50 °C. The degree of crude oil degradation by Gordonia sp. 1D at 45 °C was 38% in liquid medium and 40% in soil (with regard to abiotic loss)...
June 27, 2018: Folia Microbiologica
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