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Folia Microbiologica

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https://www.readbyqxmd.com/read/28667598/comparison-of-two-bacterial-dna-extraction-methods-from-non-polluted-and-polluted-soils
#1
Mélanie Mazziotti, Sonia Henry, Philippe Laval-Gilly, Antoine Bonnefoy, Jaïro Falla
DNA extraction from soil samples is a critical step for molecular biology analyses. The present study compared the efficiency of two DNA isolation methods from non-polluted and polluted soils with or without the presence of a plant. Both applied methods used chemical and physical lyses, but method 1 had an additional physical disruption. The main difference between these two methods was the humic acid purification technique as it was carried out during cell lysis for method 1 and after cell lysis for method 2...
July 1, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28646380/isolation-selection-and-biological-characterization-research-of-highly-effective-electricigens-from-mfcs-for-phenol-degradation
#2
Guo Wei, Du Xia, Wang Li-Li, Yan Hong
The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bacillus cereus. The effects of pH, temperature, concentrations of phenol, heavy metal ions, and salt on the growth of strain as well as the degradation of phenol have been carefully studied. The WL027-strain exhibited favorable tolerance for the metal cations including Cr(2+), Co(2+), Pb(2+), and Cu(2+) with the concentration of 0...
June 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28631154/soil-derived-organic-particles-and-their-effects-on-the-community-of-culturable-microorganisms
#3
Lukáš Bystrianský, Marcel Štofik, Milan Gryndler
Soil microbial community interacts with a range of particulate material in the soil, consisting of both inorganic and organic compounds with different levels of water solubility. Though sparingly water-soluble and insoluble organic compounds in the soil may affect living organisms, they are difficult to introduce into microbiological media. Their biological activity (i.e., their effect on soil microorganisms) thus has been almost neglected in most of the cultivation assays. To fill this gap, we propose the use of fine organic particles prepared from soil organic matter that are introduced into a laboratory medium where microbial community is cultivated...
June 19, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28623536/comparison-of-fungicidal-properties-of-non-thermal-plasma-produced-by-corona-discharge-and-dielectric-barrier-discharge
#4
J Julák, H Soušková, V Scholtz, E Kvasničková, D Savická, V Kříha
The inactivation of four micromycete species by action of non-thermal plasma was followed. Two sources of plasma were compared, namely, positive corona discharge and dielectric barrier discharge. The corona discharge appeared as suitable for fungal spore inactivation in water suspension, whereas the barrier discharge inactivated spores on the surface of cultivation agar. Cladosporium sphaerospermum was the most sensitive, being inactivated within 10 min of exposure to plasma, whereas Aspergillus oryzae displayed decrease in viable cell count only, the complete inactivation was not achieved even after 40 min of exposure...
June 17, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28597345/antimicrobial-effect-of-okcel%C3%A2-h-d-prepared-from-oxidized-cellulose
#5
Petra Moťková, Iveta Brožková, Jarmila Vytřasová, Rudolf Kukla
The antimicrobial effect of OKCEL® H-D, a topical, absorbable hemostatic textile prepared from oxidized cellulose, was tested. Testing by dilution and diffusion methods was conducted on a spectrum of 27 select microorganisms, including also antibiotic-resistant strains. OKCEL® H-D showed inhibitory effects on nearly all tested bacteria. In testing using the dilution suspension method, the majority of bacteria showed decrease in cell density by 7-8 orders of magnitude after just 6 h of exposure. For clinical isolates of antibiotic-resistant strains, a reduction occurred after 24 h of exposure...
June 8, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28551852/cultivable-microscopic-fungi-from-an-underground-chemosynthesis-based-ecosystem-a-preliminary-study
#6
Alena Nováková, Vít Hubka, Šárka Valinová, Miroslav Kolařík, Alexandra Maria Hillebrand-Voiculescu
Movile Cave, a unique groundwater ecosystem in southern Romania, was discovered in 1986. This chemoautotrophic cave contains an abundant and diverse fauna with terrestrial and aquatic invertebrate communities, including 33 endemic species. Since its discovery, studies have focused mainly on cave chemoautotrophic bacteria, while the microfungal community has been largely neglected. In this study, we determined the microfungal species living on various substrates in Movile Cave and compared this spectrum with the mycobiota detected outside the cave (outside air-borne and soil-borne microfungi)...
May 28, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28550367/in-vitro-study-of-antioxidant-and-antibacterial-activities-of-lactobacillus-probiotic-spp
#7
Z Pourramezan, R Kasra Kermanshahi, M Oloomi, A Aliahmadi, H Rezadoost
This study investigated the influence of aeration and minimal medium conditions on antioxidant and antibacterial activities of 21 probiotic Lactobacillus strains isolated from dairy products. The probiotic potential of the isolates was evaluated by pH and bile tolerance. Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to confirm the phenotypic identification of isolates. Antioxidant producer isolates were screened by resistance to reactive oxygen species (ROS). The antioxidant and antibacterial activities of extracellular materials after 48 h fermentation with antioxidative strains were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and broth microdilution assays, respectively...
May 26, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28540585/antibiofilm-activity-and-mode-of-action-of-dmso-alone-and-its-combination-with-afatinib-against-gram-negative-pathogens
#8
Mohd Fakharul Zaman Raja Yahya, Zazali Alias, Saiful Anuar Karsani
Biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. Enclosed in a self-produced extracellular polymeric substance (EPS) matrix, the biofilms often cause persistent infections. The objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (DMSO) and afatinib against Gram-negative pathogens. Test microorganisms used in this study were Escherichia coli ATCC 1299, Pseudomonas aeruginosa ATCC 10145, and Salmonella typhimurium ATCC 14028...
May 24, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28534230/trueperella-pyogenes-isolated-from-a-brain-abscess-of-an-adult-roebuck-capreolus-capreolus
#9
Jörn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Omar Hashim Sheet, Tobias Eisenberg, Osama Sammra, Mazen Alssahen, Christoph Lämmler, Ellen Prenger-Berninghoff, Michael Zschöck, Markus Timke, Amir Abdulmawjood
The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S-23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay...
May 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28516267/methods-of-candida-dubliniensis-identification-and-its-occurrence-in-human-clinical-material
#10
Martina Mahelová, Filip Růžička
Candida dubliniensis was reported as a new species in 1995. This species is often misidentified as Candida albicans. The aims of this work were to determine the occurrence of C. dubliniensis in various clinical materials, to evaluate several ways to identify it and to examine the genetic variability of isolates. Among 7706 isolates originally identified as C. albicans, 237 were identified as C. dubliniensis (3.1%). Most of the C. dubliniensis isolates were obtained from the upper and lower respiratory tract (61...
May 17, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28497337/identification-of-calmodulin-binding-proteins-in-the-entomopathogenic-fungus-beauveria-bassiana
#11
Jiyoung Kim, Junsang Oh, Deok-Hyo Yoon, Gi-Ho Sung
Calmodulin (CaM) is a primary Ca(2+) receptor and plays a pivotal role in a variety of cellular responses in eukaryotes. Even though a large number of CaM-binding proteins are well known in yeast, plants, and animals, little is known regarding CaM-targeted proteins in filamentous fungi. To identify CaM-binding proteins in filamentous fungi, we used a proteomics method coupled with co-immunoprecipitation (CoIP) and MALDI-TOF/TOF mass spectrometry (MS) in Beauveria bassiana. Through this method, we identified ten CaM-binding proteins in B...
May 11, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28451946/using-phenotype-microarrays-in-the-assessment-of-the-antibiotic-susceptibility-profile-of-bacteria-isolated-from-wastewater-in-on-site-treatment-facilities
#12
Łukasz Jałowiecki, Joanna Chojniak, Elmar Dorgeloh, Berta Hegedusova, Helene Ejhed, Jörgen Magnér, Grażyna Płaza
The scope of the study was to apply Phenotype Biolog MicroArray (PM) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. In the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. In the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria were 53, 56, and 42%, respectively...
April 27, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28124783/diversity-of-culturable-bacterial-communities-in-the-intestinal-tracts-of-goldfish-carassius-auratus-and-their-ability-to-produce-n-acyl-homoserine-lactone
#13
Haruo Sugita, Shun Kitao, Satoshi Narisawa, Ryosuke Minamishima, Shiro Itoi
Intestinal bacteria isolated from goldfish (Carassius auratus) were identified based on 16 ribosomal RNA (rRNA) gene sequences and screened for their ability to produce N-acyl homoserine lactone (AHL), an autoinducer of the quorum sensing (QS) system. The 230 aerobes/facultative anaerobes that were isolated comprised members of the genera Aeromonas (184 isolates), Citrobacter (11), Enterobacter (2), Shewanella (28), Vagococcus (1), and Vibrio (4). Among these genera, the two most abundant species were Aeromonas veronii (163 isolates) and Shewanella xiamenensis (27)...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28108870/elucidating-the-interactions-and-phytotoxicity-of-zinc-oxide-nanoparticles-with-agriculturally-beneficial-bacteria-and-selected-crop-plants
#14
Anuraag Boddupalli, Rameshwar Tiwari, Anamika Sharma, Surender Singh, Radha Prasanna, Lata Nain
There is a growing interest in the use of bioinoculants to assist mineral fertilizers in improving crop production and yield. Azotobacter and Pseudomonas are two agriculturally relevant strains of bacteria which have been established as efficient bioinoculants. An experiment involving addition of graded concentrations of zinc oxide (ZnO) nanoparticles was undertaken using log phase cultures of Azotobacter and Pseudomonas. Growth kinetics revealed a clear trend of gradual decrease with Pseudomonas; however, Azotobacter exhibited a twofold enhancement in growth with increase in the concentration of ZnO concentration...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28105601/gordonia-isolation-and-identification-in-clinical-samples-and-role-in-biotechnology
#15
REVIEW
Fatemeh Andalibi, Mehdi Fatahi-Bafghi
Gordonia spp. are members of the actinomycete family, and the environment, especially soil, is the natural habitat of this genus of bacteria. Gordonia spp. are important for two aspects: first, some Gordonia species cause a broad spectrum of diseases in healthy and immunocompromised individuals; second, these bacteria are capable of producing useful secondary metabolites, which may be used in various industries; therefore, discrimination of the genus Gordonia from other genera in the actinomycete family is important...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28097636/andrographolide-antibacterial-activity-against-common-bacteria-of-human-health-concern-and-possible-mechanism-of-action
#16
Malabika Banerjee, Debaprasad Parai, Subrata Chattopadhyay, Samir Kumar Mukherjee
Increasing bacterial resistance to common drugs is a major public health concern for the treatment of infectious diseases. Certain naturally occurring compounds of plant sources have long been reported to possess potential antimicrobial activity. This study was aimed to investigate the antibacterial activity and possible mechanism of action of andrographolide (Andro), a diterpenoid lactone from a traditional medicinal herb Andrographis paniculata. Extent of antibacterial action was assessed by minimal bactericidal concentration method...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28084600/effect-of-lantibiotic-gallidermin-against-biogenic-amine-producing-faecal-staphylococci-from-ostriches-and-pheasants
#17
Andrea Lauková, Anna Kandričáková, Pavel Pleva, Leona Buňková, Jana Ščerbová
In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococci from ostriches and pheasants and their sensitivity to lantibiotic bacteriocin gallidermin. Gallidermin belongs in a group of polycyclic proteinaceous antimicrobial substances. Thirty-six faecal staphylococci (24 strains from 140 ostriches, 12 from 60 pheasants) comprising different species were tested...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28063019/raoultella-spp-clinical-significance-infections-and-susceptibility-to-antibiotics
#18
REVIEW
Alicja Sękowska
The genus Raoultella belongs to the family of Enterobacteriaceae. Raoultella spp. are Gram-negative, aerobic, non-motile rods. This genus can be distinguished from the genus Klebsiella, in that genus use histamine as the only source of carbon in the medium. Also, Raoultella grow at 4 °C and do not produce gas from lactose at 44.5 °C. Raoultella sp. is known to inhabit natural environments (water, soil, plants). The reservoir of Raoultella is the gastrointestinal tract and upper respiratory tract. Raoultella spp...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28025801/chromium-tolerance-oxidative-stress-response-morphological-characteristics-and-ftir-studies-of-phytopathogenic-fungus-sclerotium-rolfsii
#19
Shahid Rafi, Amna Shoaib, Zoia Arshad Awan, Nayab Batool Rizvi, Nafisa, Muhammad Shafiq
Sclerotium rolfsii is one of the most destructive fungal plant pathogens that can infect over 500 plants and can adapt to diverse environmental conditions. The present research work was carried out to evaluate the impact of both hexa- and trivalent chromium (Cr) on growth, morphology, enzymatic characteristics, and metal accumulation in S. rolfsii under laboratory conditions. Experiments were performed in both malt extract broth and agar growth medium amended with six different concentrations (10, 20, 40, 60, 80, and 100 ppm) of each Cr(III) and Cr(VI) ions inoculated with fungus and incubated for 6-7 days at 25 ± 3 °C...
May 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/27988836/an-intra-laboratory-cultural-and-real-time-pcr-method-comparison-and-evaluation-for-the-detection-of-subclinical-paratuberculosis-in-dairy-herds
#20
COMPARATIVE STUDY
Annet Heuvelink, Abdulwahed Ahmed Hassan, Hilmar van Weering, Erik van Engelen, Michael Bülte, Ömer Akineden
Mycobacterium avium subsp. paratuberculosis (MAP) is a vigorous microorganism which causes incurable chronic enteritis, Johne's disease (JD) in cattle. A target of control programmes for JD is to accurately detect MAP-infected cattle early to reduce disease transmission. The present study evaluated the efficacy of two different cultural procedures and a TaqMan real-time PCR assay for detection of subclinical paratuberculosis in dairy herds. Therefore, sixty-one faecal samples were collected from two Dutch dairy herds (n = 40 and n = 21, respectively) which were known to be MAP-ELISA positive...
May 2017: Folia Microbiologica
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