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Folia Microbiologica

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https://www.readbyqxmd.com/read/29134547/detection-of-virulence-related-genes-in-lactococcus-garvieae-and-their-expression-in-response-to-different-conditions
#1
Giovanni Eraclio, Giovanni Ricci, Mattia Quattrini, Paolo Moroni, Maria Grazia Fortina
Lactococcus garvieae has emerged as an important zoonotic pathogen. However, information regarding mechanisms and factors related to its pathogenicity is lacking. In the present study, we investigated the distribution and functionality of genes related to virulence factors in L. garvieae strains isolated from different niches (diseased fish, humans, meat and dairy products, vegetables), using both post-genomic and genotypic analysis. Putative genes encoding hemolysin, fibronectin-binding protein, and penicillin acylase were detected in all analyzed genomes/strains...
November 13, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29134546/effect-of-hyperbaric-air-on-endotoxin-from-bacteroides-fragilis-strains
#2
Dittmar Chmelař, Adéla Kašíková, Petrana Martineková, Michal Hájek, Miroslav Rozložník, Marek Brabec, Jana Janečková, Jana Vobejdová, Ivan Čižnár
The aim of the project was to determine any effect of hyperbaric air on Bacteroides fragilis strains cultivated under hyperbaric conditions. Previously, it was hypothesized that there was a correlation between the presence of Bacteroides bacteria in patients preferring a meaty diet and cancer of the small intestine, and particularly of the large intestine and rectum. With respect to the fact that Bacteroides fragilis (BAFR) group are important producers of endotoxins, measurement and statistical evaluation of endotoxin production by individual strains of isolated Bacteroides species were used to compare bacteria isolated from various clinical samples from patients with colon and rectum cancer in comparison with strains isolated from other non-cancer diagnoses...
November 13, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29127620/potential-of-the-strain-raoultella-sp-kdf8-for-removal-of-analgesics
#3
Andrea Palyzová, Jiří Zahradník, Helena Marešová, Lucie Sokolová, Eva Kyslíková, Michal Grulich, Václav Štěpánek, Tomáš Řezanka, Pavel Kyslík
The bacterial strain KDF8 capable of growth in the presence of diclofenac and codeine analgesics was obtained after chemical mutagenesis of nature isolates from polluted soils. The strain KDF8 was identified as Raoultella sp. based on its morphology, biochemical properties, and 16S rRNA gene sequence. It was deposited in the Czech Collection of Microorganisms under the number CCM 8678. A growing culture efficiently removed diclofenac (92% removal) and partially also codeine (about 30% degradation) from culture supernatants within 72 h at 28 °C...
November 11, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29127619/changes-in-the-lung-bacteriome-in-relation-to-antipseudomonal-therapy-in-children-with-cystic-fibrosis
#4
Lenka Kramná, Pavel Dřevínek, Jake Lin, Michal Kulich, Ondrej Cinek
The lung in cystic fibrosis (CF) is home to numerous pathogens that shorten the lives of patients. The aim of the present study was to assess changes in the lung bacteriome following antibiotic therapy targeting Pseudomonas aeruginosa in children with CF. The study included nine children (9-18 years) with CF who were treated for their chronic or intermittent positivity for Pseudomonas aeruginosa. The bacteriomes were determined in 16 pairs of sputa collected at the beginning and at the end of a course of intravenous antibiotic therapy via deep sequencing of the variable region 4 of the 16S rRNA gene, and the total bacterial load and selected specific pathogens were assessed using quantitative real-time PCR...
November 10, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29116587/a-simplified-globally-affordable-experimental-setup-for-monitoring-dna-diagnosis-by-a-qd-based-technique
#5
Samira Rostam Gohari, Razieh Yazdanparast
The unavailability of simple, quick, and sensitive genetic-based molecular diagnostic techniques has become the main driving force for inventing new approaches in the era of quantum dots (QDs): a new class of fluorescent probes with fascinating optical electronic properties. Using the unique size-dependent light-emitting properties of QDs, we have developed a QD-based ultrasensitive technique which removes the necessity for the genetic amplification step required in almost all types of molecular-based diagnostic techniques...
November 8, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29116586/correction-to-acquisition-of-tn6018-3-cs-regions-increases-colistin-mics-against-acinetobacter-baumannii-isolates-harboring-new-variants-of-abars
#6
Mohammad Savari, Alireza Ekrami, Saeed Shoja, Abbas Bahador
The published online version of this article contained a mistake. The correct affiliation of Alireza Ekrami should have been "Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran" . The authors regret this error.
November 7, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29079936/the-current-status-of-aureobasidium-pullulans-in-biotechnology
#7
REVIEW
Sehanat Prasongsuk, Pongtharin Lotrakul, Imran Ali, Wichanee Bankeeree, Hunsa Punnapayak
Different strains of the saprophytic yeast-like fungus Aureobasidium pullulans (Ascomycota: Dothideales) exhibit different biochemical characteristics, while their ubiquitous occurrence across diverse habitats and environmental conditions makes them an easily accessible source for biotechnological exploitation. They are useful in agricultural and industrial applications. Their antagonistic activities against postharvest pathogens make them suitable bioagents for the postharvest preservation of fruits and vegetables, while they possess antimicrobial activities against bacteria and fungi...
October 27, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29076050/correction-to-diversity-of-culturable-bacterial-communities-in-the-intestinal-tracts-of-goldfish-carassius-auratus-and-their-ability-to-produce-n-acyl-homoserine-lactone
#8
Haruo Sugita, Shun Kitao, Satoshi Narisawa, Ryosuke Minamishima, Shiro Itoi
In the original publication, the number of the NCTC strain used was published incorrectly. The authors apologize for the error. The error has no effect on the validity of the results and is corrected for accuracy.
October 26, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/29052811/four-saccharomyces-species-differ-in-their-tolerance-to-various-stresses-though-they-have-similar-basic-physiological-parameters
#9
Jana Zemančíková, Marie Kodedová, Klára Papoušková, Hana Sychrová
Saccharomyces species, which are mostly used in the food and beverage industries, are known to differ in their fermentation efficiency and tolerance of adverse fermentation conditions. However, the basis of their difference has not been fully elucidated, although their genomes have been sequenced and analyzed. Five strains of four Saccharomyces species (S. cerevisiae, S. kudriavzevii, S. bayanus, and S. paradoxus), when grown in parallel in laboratory conditions, exhibit very similar basic physiological parameters such as membrane potential, intracellular pH, and the degree to which they are able to quickly activate their Pma1 H(+)-ATPase upon glucose addition...
October 20, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28983822/in-vitro-activity-of-micafungin-against-biofilms-of-candida-albicans-candida-glabrata-and-candida-parapsilosis-at-different-stages-of-maturation
#10
Małgorzata Prażyńska, Tomasz Bogiel, Eugenia Gospodarek-Komkowska
Candida spp. is able to form a biofilm, which is considered resistant to the majority of antifungals used in medicine. The aim of this study was to evaluate the in vitro activity of micafungin against Candida spp. biofilms at different stages of their maturation (2, 6, and 24 h). We assessed the inhibitory effect of micafungin against 78 clinical isolates of Candida spp., growing as planktonic or sessile cells, by widely recommended broth microdilution method. The in vitro effect on sessile cells viability was evaluated by colorimetric reduction assay...
October 5, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28971316/resveratrol-pterostilbene-and-baicalein-plant-derived-anti-biofilm-agents
#11
Irena Kolouchová, Olga Maťátková, Martina Paldrychová, Zdeněk Kodeš, Eva Kvasničková, Karel Sigler, Alena Čejková, Jan Šmidrkal, Kateřina Demnerová, Jan Masák
Microbial adhesion to surfaces and the subsequent biofilm formation may result in contamination in food industry and in healthcare-associated infections and may significantly affect postoperative care. Some plants produce substances with antioxidant and antimicrobial properties that are able to inhibit the growth of food-borne pathogens. The aim of our study was to evaluate antimicrobial and anti-biofilm effect of baicalein, resveratrol, and pterostilbene on Candida albicans, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli...
October 2, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28965320/detection-of-coxiella-burnetii-dna-and-anti-coxiella-burnetii-igg-antibodies-in-precolostral-blood-samples-of-stillborn-calves-in-an-endemically-infected-holstein-dairy-herd
#12
Markus Freick, Andrea Konrath, Haimo Enbergs, Jörg Walraph, Jim Weber, Karin Eulenberger
Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, occurs widely in cattle herds. After invasion of the pregnant uterus and initial localization in the placenta, active C. burnetii infections may spread to the fetus hematogenously or by the amniotic-oral route and thus may cause abortion, premature delivery, stillbirth, and weak offspring (APSW) complex. In a case-control study, we investigated precolostral blood samples of 56 stillborn calves and 30 live births from a dairy herd endemically infected with C...
September 30, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28956275/infection-of-recurrent-calcaneal-ulcer-caused-by-a-biofilm-producer-myroides-odoratimimus-strain
#13
Arianna Pompilio, Giuseppe Galardi, Giovanni Gherardi, Fabio Verginelli, Cristina Geminiani, Argenia Paola Pilloni, Piergiorgio Catalanotti, Giovanni Di Bonaventura
We report a case of recurrent post-traumatic ulcer infection due to Myroides odoratimimus in an immunocompromised male. We have also reviewed the medical literature on isolated M. odoratimimus infections. The strain, isolated from ulcer discharge, was multidrug-resistant and treatment with meropenem, based on susceptibility testing, led to resolution of infection. The strain was also able to form a relevant amount of biofilm over time, thus suggesting a possible role of sessile communities in the chronicization of infection...
September 27, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28942582/analysis-of-streptomyces-coelicolor-m145-genes-sco4164-and-sco5854-encoding-putative-rhodaneses
#14
Tetiana Gren, Bohdan Ostash, Volodymyr Babiy, Ihor Rokytskyy, Victor Fedorenko
Streptomyces coelicolor genome carries two apparently paralogous genes, SCO4164 and SCO5854, that encode putative thiosulfate sulfurtransferases (rhodaneses). These genes (and their presumed translation products) are highly conserved and widely distributed across actinobacterial genomes. The SCO4164 knockout strain was unable to grow on minimal media with either sulfate or sulfite as the sole sulfur source. The SCO5854 mutant had no growth defects in the presence of various sulfur sources; however, it produced significantly less amounts of actinorhodin...
September 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28940128/multi-locus-sequence-typing-mlst-of-non-fermentative-gram-negative-bacilli-isolated-from-bloodstream-infections-in-southern-poland
#15
Agnieszka Chmielarczyk, Monika Pobiega, Dorota Romaniszyn, Jadwiga Wójkowska-Mach
Non-fermentative Gram-negative bacilli are now one of the most important causes of severe infections in Polish hospitals. Acinetobacter species are serious concern because of the high prevalence of multi-drug resistance among strains. Resistance profiles for 53 Gram-negative non-fermentative blood isolates were done. MLST was carried out using 44 strains representing the most commonly isolated species: A. baumannii, P. aeruginosa, and S. maltophilia. MLST revealed that all 22 A. baumannii belonged to sequence type (ST) 2...
September 22, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28913757/mycobacterium-shimoidei-cavitary-pulmonary-disease-with-favorable-outcome
#16
Vide Popovic, Dragan Arar, Dragica Roso Popovic, Igor Barisic, Marija Tonkic, Irena Peric, Anita Novak, Zana Rubic, Vera Katalinic-Jankovic, Mateja Jankovic Makek, Ivana Goic-Barisic
We report a case of cavitary pulmonary disease caused by Mycobacterium shimoidei in 67-year-old female with history of asthma. Even though susceptibility testing was not available, choice of treatment regimen (streptomycin, rifampicin, ethambutol, and clarithromycin), based on a few cases with favorable outcome reported in the literature, resulted with an excellent clinical, microbiological, and radiological response. This is the first report of pulmonary disease caused by M. shimoidei, but also the first ever isolation of M...
September 14, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28905285/effect-of-bacteriocin-and-exopolysaccharides-isolated-from-probiotic-on-p-aeruginosa-pao1-biofilm
#17
Vivek Sharma, Kusum Harjai, Geeta Shukla
Microorganisms develop biofilms on indwelling medical devices and are associated with biofilm-related infections, resulting in substantial morbidity and mortality. Therefore, to prevent and control biofilm-associated infections, the present study was designed to assess the anti-biofilm potential of postbiotics derived from probiotic organisms against most prevalent biofilm-forming Pseudomonas aeruginosa PAO1. Eighty lactic acid bacteria isolated from eight neonatal fecal samples possessed antibacterial activity against P...
September 14, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28891002/fusarium-equiseti-lpsc-1166-and-its-in-vitro-role-in-the-decay-of-heterostachys-ritteriana-leaf-litter
#18
Ernesto Franco, María I Troncozo, Margot Baez, María V Mirífico, Gerardo L Robledo, Pedro A Balatti, Mario C N Saparrat
The role of microorganisms in litter degradation in arid and semi-arid zones, where soil and water salinization is one of the main factors limiting carbon turnover and decay, remains obscure. Heterostachys ritteriana (Amaranthaceae), a halophyte shrub growing in arid environments such as "Salinas Grandes" (Córdoba, Argentina), appears to be the main source of organic matter in the area. Little is known regarding the microorganisms associated with H. ritteriana, although they are a potential source of enzymes such as cellulolytic ones, which might be important in biotechnological fields such as bioethanol production using ionic liquids...
September 10, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28889401/severity-of-drug-resistance-and-co-existence-of-enterococcus-faecalis-in-diabetic-foot-ulcer-infections
#19
Kavitha Shettigar, Deepika V Bhat, Kapaettu Satyamoorthy, Thokur Sreepathy Murali
The genes encoding aminoglycoside resistance in Enterococcus faecalis may promote collateral aminoglycoside resistance in polymicrobial wounds. We studied a total of 100 diabetic foot ulcer samples for infection and found 60 samples to be polymicrobial, 5 to be monomicrobial, and 35 samples to be culture negative. A total of 65 E. faecalis isolates were screened for six genes coding for aminoglycoside resistance, antibiotic resistance patterns, and biofilm production. Infectious Diseases Society of America/International Working Group on the Diabetic Foot system was used to classify the wound ulcers...
September 9, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28887734/mating-of-natural-saccharomyces-cerevisiae-strains-for-improved-glucose-fermentation-and-lignocellulosic-inhibitor-tolerance
#20
Trudy Jansen, Justin Wallace Hoff, Neil Jolly, Willem Heber van Zyl
Natural Saccharomyces cerevisiae isolates from vineyards in the Western Cape, South Africa were evaluated for ethanol production in industrial conditions associated with the production of second-generation biofuels. The strains displayed high phenotypic diversity including the ability to grow at 45 °C and in the presence of 20% (v/v) ethanol, strain YI13. Strains HR4 and YI30 were inhibitor-tolerant under aerobic and oxygen-limited conditions, respectively. Spore-to-spore hybridization generated progeny that displayed heterosis, including increased ethanol productivity and improved growth in the presence of a synthetic inhibitor cocktail...
September 8, 2017: Folia Microbiologica
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