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Journal of Clinical Pathology

Hong-Qing Cai, Peng-Fei Wang, Hai-Peng Zhang, Zhi-Jian Cheng, Shou-Wei Li, Jie He, Yu Zhang, Jia-Jie Hao, Ming-Rong Wang, Chang-Xiang Yan, Jing-Hai Wan
AIM: To identify biomarkers for accurate classification of glioma. PATIENTS AND METHODS: We evaluated the heat shock protein 27 (Hsp27), phosphorylated Hsp27 (p-Hsp27), ATRX and IDH1R132H proteins using immunohistochemistry in 421 glioma tissues. The χ2 test was used to assess the relationship between molecular alterations and clinico-pathological parameters. Kaplan-Meier survival curves were constructed, and differences were detected by the log-rank test. RESULTS: We found that Hsp27 and p-Hsp27 were mainly expressed in aggressive astrocytic gliomas...
March 17, 2018: Journal of Clinical Pathology
Heather L Howie, Xiaohong Wang, Linda Kapp, Jenna N Lebedev, James C Zimring
There are four subtypes of human IgG with different effector functions. Quantifying the relative amount of each IgG subtype is important for laboratory diagnosis in multiple settings. However, in an evolving landscape of the appreciation of human variability and the need for precision/personalised laboratory diagnosis, it has also been shown that there are numerous natural variants of IgG subtypes, with at least 29 having been described. It has recently been reported that commercially available polyclonal antisera to IgG3 cross react with variants of other IgG subtypes, while available monoclonal anti-IgG3 have a blind-spot for the IgG3-04 variant...
March 17, 2018: Journal of Clinical Pathology
Bethany Jill Williams, David Bottoms, David Clark, Darren Treanor
Diagnostic histopathology departments are experiencing unprecedented economic and service pressures, and many institutions are now considering digital pathology as part of the solution. In this document, a follow on to our case for adoption report, we provide information and advice to help departments create their own clear, succinct, individualised business case for the clinical deployment of digital pathology.
March 16, 2018: Journal of Clinical Pathology
James Wiggins, Emily Hayes, Marc Heymann, Rand Hawari, Richard A Carr
No abstract text is available yet for this article.
March 16, 2018: Journal of Clinical Pathology
Antonio La Gioia, Maurizio Fumi, Fabiana Fiorini, Paola Pezzati, Fiamma Balboni, Maria Bombara, Alessandra Marini, Ylenia Pancione, Leonardo Solarino, Elisa Marchese, Silvia Sale, Vincenzo Rocco, Marcello Fiorini
AIMS: The presence of cold agglutinin in blood samples can cause a spontaneous agglutination of red blood cells (RBCs) when low temperature occurs. This phenomenon causes a spurious lowering of RBC count on the automated haematological analysers that are detected by incongruous values (≥370 g/L) of the mean cellular haemoglobi concentration (MCHC). A preheating at 37°C can remove the RBC agglutination generally resulting in a reliable count. It has been reported that the same result can be reached by using the optical reticulocyte (RET) channel of Sysmex analysers where the RBC count is not influenced by the presence of cold agglutinin...
March 13, 2018: Journal of Clinical Pathology
Brice Autier, Sarah Dion, Florence Robert-Gangneux
AIM: Toxoplasmosis following liver transplant with donor-recipient mismatch is rare, but is often life-threatening. However, there are no data on the frequency of cyst carriage in the liver, nor consensual chemoprophylaxis guidelines. This study aimed at describing frequency and localisation of Toxoplasma cysts in the liver in a mouse model of chronic infection to predict the risk in liver transplantation. METHODS: Heart, brain and liver lobes of 21 mice chronically infected with Toxoplasma were collected for DNA extraction and amplification of Toxoplasma gondii rep529 sequence by real-time PCR...
March 13, 2018: Journal of Clinical Pathology
Emad A Rakha, Mohamed Aleskandarani, Michael S Toss, Andrew R Green, Graham Ball, Ian O Ellis, Leslie W Dalton
AIMS: Virtual microscopy utilising digital whole slide imaging (WSI) is increasingly used in breast pathology. Histologic grade is one of the strongest prognostic factors in breast cancer (BC). This study aims at investigating the agreement between BC grading using traditional light microscopy (LM) and digital WSI with consideration of reproducibility and impact on outcome prediction. METHODS: A large (n=1675) well-characterised cohort of BC originally graded by LM was re-graded using WSI...
March 13, 2018: Journal of Clinical Pathology
Caterina Fumagalli, Davide Vacirca, Alessandra Rappa, Antonio Passaro, Juliana Guarize, Paola Rafaniello Raviele, Filippo de Marinis, Lorenzo Spaggiari, Chiara Casadio, Giuseppe Viale, Massimo Barberis, Elena Guerini-Rocco
BACKGROUND: Molecular profiling of advanced non-small cell lung cancers (NSCLC) is essential to identify patients who may benefit from targeted treatments. In the last years, the number of potentially actionable molecular alterations has rapidly increased. Next-generation sequencing allows for the analysis of multiple genes simultaneously. AIMS: To evaluate the feasibility and the throughput of next-generation sequencing in clinical molecular diagnostics of advanced NSCLC...
March 13, 2018: Journal of Clinical Pathology
Nicole Matejckova, Alena Zajicova, Barbora Hermankova, Jan Kossl, Pavla Bohacova, Vladimir Holan, Eliska Javorkova
AIMS: Mesenchymal stem cells (MSCs) have recently been tested in clinical trials to treat severe diseases, including amyotrophic lateral sclerosis (ALS). Since autologous MSCs are frequently used for therapy, we aimed to evaluate the possible influence of the disease on characteristics and function of these cells. METHODS: MSCs were isolated from the bone marrow of patients with ALS and compared with MSCs from healthy controls (HC). The cells were tested for phenotype, growth properties, differentiation ability, metabolic activity, secretory potential, expression of genes for immunomodulatory molecules and for the ability to regulate proliferation of mitogen-stimulated peripheral blood leucocytes...
March 9, 2018: Journal of Clinical Pathology
Liuwei Gao, Hua Zhang, Bin Zhang, Jinfang Zhu, Chen Chen, Weiran Liu
OBJECTIVE: The aim of this study was to evaluate the expression of beta-1,3-N-acetylglucosaminyltransferase-3 (B3GNT3) in non-small cell lung cancer (NSCLC) patients and to investigate the relevance of B3GNT3 expression in tumour prognosis. METHODS: In this study, B3GNT3 expression was examined in five pairs of resectable NSCLC tissue by Western blot and in 42 pairs of resectable NSCLC tissue by quantitative real-time PCR (qRT-PCR). Immunohistochemistry and statistical analysis were performed to assess the relationship between B3GNT3 expression scores and clinicopathological parameters, as well as clinical prognosis in a retrospective cohort of 176 NSCLC patients...
February 26, 2018: Journal of Clinical Pathology
Susanti Susanti, Wakkas Fadhil, Henry Okuchukwu Ebili, Abutaleb Asiri, Ausrine Nestarenkaite, Efthymios Hadjimichael, Hersh A Ham-Karim, Joanne Field, Katherine Stafford, Balwir Matharoo-Ball, James C Hassall, Abid Sharif, Anca Oniscu, Mohammad Ilyas
AIMS: We sought to use PCR followed by high-resolution melting analysis to develop a single closed-tube screening panel to screen for Lynch syndrome. This comprises tests for microsatellite instability (MSI), MLH1 methylation promoter and BRAF mutation. METHODS: For MSI testing, five mononucleotide markers (BAT25, BAT26, BCAT25, MYB , EWSR1 ) were developed. In addition, primers were designed to interrogate Region C of the MLH1 promoter for methylation (using bisulphite-modified DNA) and to test for mutations in codon 600 of BRAF...
February 22, 2018: Journal of Clinical Pathology
Karl Haslam, Sarah Dell, Emer Atkinson, Helen Enright, Patrick Thornton, Stephen E Langabeer
No abstract text is available yet for this article.
February 20, 2018: Journal of Clinical Pathology
Harry R Haynes, Clare L Killick-Cole, Kelly M Hares, Juliana Redondo, Kevin C Kemp, Karwan A Moutasim, Claire Faulkner, Alastair Wilkins, Kathreena M Kurian
AIMS: Histopathological tissue samples are being increasingly used as sources of nucleic acids in molecular pathology translational research. This study investigated the suitability of glioblastoma and control central nervous system (CNS) formalin-fixed paraffin embedded (FFPE) tissue-derived RNA for gene expression analyses. METHODS: Total RNA was extracted from control (temporal lobe resection tissue) and glioblastoma FFPE tissue samples. RNA purity (260/280 ratios) was determined and RNA integrity number (RIN) analysis was performed...
February 20, 2018: Journal of Clinical Pathology
Zuzana Chyra Kufova, Tereza Sevcikova, Jaroslav Januska, Petr Vojta, Arpad Boday, Pavla Vanickova, Jana Filipova, Katerina Growkova, Tomas Jelinek, Marian Hajduch, Roman Hajek
AIMS: Amyloidosis is caused by deposition of abnormal protein fibrils, leading to damage of organ function. Hereditary amyloidosis represents a monogenic disease caused by germline mutations in 11 amyloidogenic precursor protein genes. One of the important but non-specific symptoms of amyloidosis is hypertrophic cardiomyopathy. Diagnostics of hereditary amyloidosis is complicated and the real cause can remain overlooked. We aimed to design hereditary amyloidosis gene panel and to introduce new next-generation sequencing (NGS) approach to investigate hereditary amyloidosis in a cohort of patients with hypertrophic cardiomyopathy of unknown significance...
February 17, 2018: Journal of Clinical Pathology
Mingxuan Lin, Peak Ling Lee, Lily Chiu, Constance Chua, Kenneth H K Ban, Adeline H F Lin, Zit Liang Chan, Tae-Hoon Chung, Benedict Yan, Wee-Joo Chng
AIMS: Multiple myeloma (MM) is a heterogeneous disease characterised by genetically complex abnormalities. The classical mutational spectrum includes recurrent chromosomal aberrations and gene-level mutations. Recurrent translocations involving the IGH gene such as t(11;14), t(4;14) and t(14;16) are well known. However, the presence of complex genetic abnormalities raises the possibility that fusions other than the recurrent IGH translocations exist. We therefore employed a targeted RNA-sequencing panel to identify novel putative fusions in a local cohort of MM...
February 16, 2018: Journal of Clinical Pathology
Rachel S Newby, Matthew Dryden, Raymond N Allan, Rami J Salib
The opportunistic pathogen non-typeable Haemophilus influenzae (NTHi) plays an important role in many chronic respiratory diseases including otitis media, chronic rhinosinusitis, cystic fibrosis and chronic obstructive pulmonary disease. Biofilm formation has been implicated in NTHi colonisation, persistence of infection and recalcitrance towards antimicrobials. There is therefore a pressing need for the development of novel treatment strategies that are effective against NTHi biofilm-associated diseases. SurgihoneyRO is a honey-based product that has been bioengineered to enable the slow release of H2 O2 , a reactive oxygen species to which H...
February 15, 2018: Journal of Clinical Pathology
Benjamin F Dessauvagie, Andrew H S Lee, Katie Meehan, Anju Nijhawan, Puay Hoon Tan, Jeremy Thomas, Bibiana Tie, Darren Treanor, Seemeen Umar, Andrew M Hanby, Rebecca Millican-Slater
AIM: Fibroepithelial lesions (FELs) of the breast span a morphological continuum including lesions where distinction between cellular fibroadenoma (FA) and benign phyllodes tumour (PT) is difficult. The distinction is clinically important with FAs managed conservatively while equivocal lesions and PTs are managed with surgery. We sought to audit core biopsy diagnoses of equivocal FELs by digital pathology and to investigate whether digital point counting is useful in clarifying FEL diagnoses...
February 13, 2018: Journal of Clinical Pathology
Newton A C S Wong, Fernanda Amary, Rachel Butler, Richard Byers, David Gonzalez, Harry R Haynes, Mohammad Ilyas, Manuel Salto-Tellez, Philippe Taniere
The use of biologics targeted to the human epidermal growth factor receptor 2 (HER2) protein is the latest addition to the armamentarium used to fight advanced gastric or gastro-oesophageal junction adenocarcinoma. The decision to treat with the biologic trastuzumab is completely dependent on HER2 testing of tumour tissue. In 2017, the College of American Pathologists, American Society for Clinical Pathology and the American Society of Clinical Oncology jointly published guidelines for HER2 testing and clinical decision making in gastro-oesophageal adenocarcinoma...
February 8, 2018: Journal of Clinical Pathology
Emine Kilic Bagir, Arbil Acikalin, Alper Avci, Derya Gumurdulu, Semra Paydas
AIMS: We explored the relationships between programmed death-1 (PD-1) and programmed death-ligand 1 (PD-L1) expression and the pathological and clinical features of thymic epithelial tumours and thymic hyperplasia. METHODS: We evaluated PD-1 and PDL-1 expressions within epithelial and microenvironmental components in thymic epithelial tumours (n=44) and thymic hyperplasias (n=8), immunohistochemically. We compared the results with demographic, clinical and histopathological features of the cases...
February 8, 2018: Journal of Clinical Pathology
Bao-Hua Yu, Shao-Xian Tang, Xiao-Li Xu, Yu-Fan Cheng, Rui Bi, Ruo-Hong Shui, Xiao-Yu Tu, Hong-Fen Lu, Xiao-Yan Zhou, Wen-Tao Yang
AIMS: To fully elucidate the clinicopathological features of breast carcinoma in sclerosing adenosis (SA-BC). METHODS: Clinical and histological characteristics of 206 SA-BCs from 180 patients were retrospectively evaluated. Immunohistochemical phenotype was examined. The clinicopathological relevance of the topographical pattern of SA-BCs was analysed. RESULTS: Overall, up to 46 patients (25.6%) had contralateral cancer, either SA associated or not...
February 7, 2018: Journal of Clinical Pathology
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