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Journal of Clinical Pathology

Marjolein van Zummeren, Annemiek Leeman, Wieke W Kremer, Maaike C G Bleeker, David Jenkins, Miekel van de Sandt, Daniëlle A M Heideman, Renske Steenbergen, Peter J F Snijders, Wim G V Quint, Johannes Berkhof, Chris J L M Meijer
AIMS: To investigate the accuracy and reproducibility of a scoring system for cervical intraepithelial neoplasia (CIN1-3) based on immunohistochemical (IHC) biomarkers Ki-67 and p16ink4a . METHODS: 115 cervical tissue specimens were reviewed by three expert gynaecopathologists and graded according to three strategies: (1) CIN grade based on H&E staining only; (2) immunoscore based on the cumulative score of Ki-67 and p16ink4a only (0-6); and (3) CIN grade based on H&E supported by non-objectified IHC 2 weeks after scoring 1 and 2...
July 16, 2018: Journal of Clinical Pathology
Claudia Louise Craven, Miles D Chapman, Linda D'Antona, Simon D Thompson, Neghat Lakdawala, Henrik Zetterberg, Laurence D Watkins, Ahmed K Toma
No abstract text is available yet for this article.
July 11, 2018: Journal of Clinical Pathology
Luis Gai, Susan J Done, Donald Cook, Nash Denic, Polycarp Erivwo, Kim Voisey, Kenneth Kao
No abstract text is available yet for this article.
July 7, 2018: Journal of Clinical Pathology
Hiromasa Sakamoto, Toshinari Yamasaki, Takayuki Sumiyoshi, Noriaki Utsunomiya, Masashi Takeda, Tomomi Kamba, Eijiro Nakamura, Osamu Ogawa
AIM: We examined the genetic alterations in a mother and son with multiple eosinophilic chromophobe renal cell carcinomas (chRCCs) showing no other features. METHODS: Germline DNA and bilateral renal cell carcinoma DNA were genetically analysed by whole-exome sequencing. Candidate gene alterations in the first patient's germline were investigated in her child's germline and the chRCCs. RESULTS: We detected several germline gene alterations in the mother...
June 30, 2018: Journal of Clinical Pathology
Deirdre Mary Timlin, Kevin O'Hare, Jan Walker, Giusseppa Castriciano, Yvonne Connolly, Cliona Grant, Christopher Larry Bacon, Elisabeth Vandenberghe, Barbara Dunne, Michael Jeffers, Richard Flavin
No abstract text is available yet for this article.
June 30, 2018: Journal of Clinical Pathology
Alberto Alvarez-Larran, Daniel Martínez, Leonor Arenillas, Ariadna Rubio, Eduardo Arellano-Rodrigo, Juan Carlos Hernández Boluda, Natalia Papaleo, Gonzalo Caballero, Clara Martínez, Francisca Ferrer-Marín, María Isabel Mata, Manuel Pérez-Encinas, María Antonia Durán, José María Alonso, Gonzalo Carreño-Tarragona, Juan Manuel Alonso, Soledad Noya, Elena Magro, Raúl Pérez, Mónica López-Guerra, Irene Pastor-Galán, Francisco Cervantes, Carlos Besses, Luis Colomo, María Rozman
AIM: To characterise the clinical and histological features of MPL -mutated essential thrombocythaemia (ET). PATIENTS AND METHODS: Bone marrow biopsies of 175 patients with ET were centrally reviewed according to the 2016 WHO classification, including 42 cases with MPL mutation, 98 JAK2 V617F-mutated and 35 CALR -mutated. Clinical and histological features were compared among the three genotypes included in the current 2016 WHO classification and among the different types of MPL mutations...
June 22, 2018: Journal of Clinical Pathology
Nicky Josman, Nancy W S Tee, Matthias Maiwald, Liat Hui Loo, Clement K M Ho
BACKGROUND: It is often impractical for each laboratory to establish its own paediatric reference intervals. This is particularly true for specimen types collected using invasive procedures, for example, cerebrospinal fluid (CSF). METHODS: Published CSF reference intervals for white cell count, and concentrations of total protein and glucose were reviewed by stakeholders in a paediatric hospital. Consensus reference intervals for the three CSF parameters were then subjected to verification using guidelines from the Clinical Laboratory Standards Institute and residual CSF specimens...
June 15, 2018: Journal of Clinical Pathology
Arnaud Uguen, Giancarlo Troncone
No abstract text is available yet for this article.
June 14, 2018: Journal of Clinical Pathology
Giuseppe Lippi, Cantor Tarperi, Martina Montagnana, Elisa Danese, Gian Luca Salvagno, Federico Schena
No abstract text is available yet for this article.
June 8, 2018: Journal of Clinical Pathology
Elena Aloisio, Assunta Carnevale, Sara Pasqualetti, Sarah Birindelli, Alberto Dolci, Mauro Panteghini
No abstract text is available yet for this article.
June 1, 2018: Journal of Clinical Pathology
James Liang, Jacques A J Malherbe, Kathryn A Fuller, Bob Mirzai, Carly George, Tina L Carter, Catherine H Cole, Belinda B Guo, Katie Meehan, Wendy N Erber
AIMS: The number of precursor and mature lymphoid cells and plasma cells in normal bone marrow trephine (BMT) biopsies throughout the human lifespan is unknown. Reference ranges have been established from aspirated marrow, but due to haemodilution errors, these do not accurately reflect the native marrow milieu. We aimed to define age-specific, normal reference ranges for lymphoid and plasma cells in BMT biopsy specimens using a combined immunophenotyping and digital enumeration approach...
June 1, 2018: Journal of Clinical Pathology
Marija Grdić Rajković, Sanja Popović-Grle, Andrea Vukić Dugac, Dunja Rogić, Ivana Rako, Margareta Radić Antolić, Mirela Beriša, Lada Rumora
AIMS: Chronic obstructive pulmonary disease (COPD) is characterised with oxidative stress. Paraoxonase 1 (PON1) is an enzyme, coded by PON1 gene, with distinctive antiatherogenic and antioxidative roles. We aimed to investigate the frequencies of Q192R, L55M and -108C>T polymorphisms and association of those polymorphisms with paraoxonase and arylesterase activities in patients with COPD. METHODS: PON1 genotype was determined by PCR-restriction fragment length polymorphism method...
June 1, 2018: Journal of Clinical Pathology
Laure Sorber, Karen Zwaenepoel, An Wouters, Annelies Janssens, Birgitta Hiddinga, Jan Van Meerbeeck, Filip Lardon, Patrick Pauwels, Christian Rolfo
No abstract text is available yet for this article.
May 31, 2018: Journal of Clinical Pathology
Jason H Kurzer, Olga K Weinberg
Differentiating malignant B-lymphoblasts from early benign B cell precursors (hematogones) is a vital component of the diagnosis of B-lymphoblastic leukaemia. It has been previously reported that only late-stage B cell precursors circulate in the peripheral blood. Consequently, flow cytometric detection of cells with immunophenotypic findings similar to earlier stage precursors in the peripheral blood justifiably raises concern for involvement by B-lymphoblastic leukaemia. We report here, however, that benign early B cell precursors can indeed be detected in the peripheral blood, thus complicating the interpretation of flow cytometric findings derived from these sample types...
May 25, 2018: Journal of Clinical Pathology
Gianluca Mauri, Emanuele Valtorta, Giulio Cerea, Alessio Amatu, Michele Schirru, Giovanna Marrapese, Vincenzo Fiorillo, Patrizia Recchimuzzo, Ivana Stella Cavenago, Erica Francesca Bonazzina, Valentina Motta, Calogero Lauricella, Silvio Veronese, Federica Tosi, Martina Maiolani, Giuseppe Rospo, Mauro Truini, Emanuela Bonoldi, Jason Christiansen, Steven J Potts, Salvatore Siena, Andrea Sartore-Bianchi
AIMS: Neurotrophic Tropomyosin Kinase Receptor 1 ( NTRK1 ) gene encodes for the protein Tropomyosin-related kinase A (TRKA). Deregulated activity of TRKA has been shown to have oncogenic potential. We present here the results of an immunohistochemical (IHC) observational cohort study of TRKA expression together with gene copy number (GCN) assessment in various solid tumours. METHODS: Formalin-fixed, paraffin-embedded consecutive samples of different tumour types were tested for TRKA expression...
May 25, 2018: Journal of Clinical Pathology
Eliane P Dias, Bruna L S Picciani, Vanessa de C B Dos Santos, Karin S Cunha
AIMS: In the diagnostic or research field, there is a need that the histopathological evaluation provides as much information as possible, with the shortest time and lowest cost. The development of the tissue microarray (TMA) technology has significantly facilitated and accelerated studies with tissue analyses using in situ technologies. Nevertheless, one of the most recognised limitations of TMA is that the small cores used to construct a TMA may not accurately represent characteristics of the whole tissue specimen...
May 24, 2018: Journal of Clinical Pathology
Caterina De Luca, Alessandra G Rappa, Gianluca Gragnano, Umberto Malapelle, Giancarlo Troncone, Massimo Barberis
AIMS: Any reference laboratory testing non-small cell lung cancer samples for predictive biomarkers needs to develop and validate a wide range of different molecular techniques, each with a specific time requirement and application. Updated international guidelines suggest that next generation sequencing (NGS) to be the initial procedure. However, in a non-negligible subset of cases, library generation may fail or amplicon coverage may be insufficient. In these NGS 'invalid' cases, the Idylla system may represent a viable option for rapid epidermal growth factor receptor ( EGFR ) genotyping...
May 24, 2018: Journal of Clinical Pathology
Kelly J Butnor, Justin Rueckert, Elizabeth N Pavlisko, Thomas A Sporn, Victor L Roggli
AIMS: Florid mesothelial hyperplasia is known to result from endometriosis. Well-differentiated papillary mesothelioma and multiloculated peritoneal inclusion cysts have also been described in women with endometriosis. To our knowledge, peritoneal diffuse malignant mesothelioma (MM) arising in the setting of endometriosis has not been reported. The purpose of this study is to report the clinicopathological characteristics of women with MM and endometriosis. METHODS: The surgical pathology files of a tertiary academic medical centre and the consultation files of one of the study authors were reviewed for cases of MM in females with and without endometriosis...
May 23, 2018: Journal of Clinical Pathology
Daniel K Faria, Leandro U Taniguchi, Luiz A M Fonseca, Mario Ferreira-Junior, Francisco J B Aguiar, Arnaldo Lichtenstein, Nairo M Sumita, Alberto J S Duarte, Maria M Sales
AIM: To detect differences in the pattern of serum calcium tests ordering before and after the implementation of a decision algorithm. METHODS: We studied patients admitted to an internal medicine ward of a university hospital on April 2013 and April 2016. Patients were classified as critical or non-critical on the day when each test was performed. Adequacy of ordering was defined according to adherence to a decision algorithm implemented in 2014. RESULTS: Total and ionised calcium tests per patient-day of hospitalisation significantly decreased after the algorithm implementation; and duplication of tests (total and ionised calcium measured in the same blood sample) was reduced by 49%...
May 18, 2018: Journal of Clinical Pathology
Khaled Alayed, Karen Schweitzer, Amad Awadallah, Shashirekha Shetty, Samir Turakhia, Howard Meyerson
AIM: Develop an objective assay to detect c-MYC protein expression using multiparametric flow cytometry (FCM) as an alternative to immunohistochemistry (IHC). METHODS: 57 patient samples and 11 cell line samples were evaluated. Cell suspensions were obtained and c-MYC staining was performed in combination with CD45 and CD19 and, in some samples, CD10. The percentage of c-MYC+ cells by FCM was correlated with the percentage determined by IHC. The relationship between c-MYC protein expression and the presence of a c-MYC gene rearrangement in aggressive and high-grade lymphomas was also assessed...
May 16, 2018: Journal of Clinical Pathology
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