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Journal of Clinical Pathology

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https://www.readbyqxmd.com/read/27899414/sentinel-case-of-richter-transformation-from-chronic-lymphocytic-leukaemia-small-lymphocytic-lymphoma-to-cd3-diffuse-large-b-cell-lymphoma
#1
Ali Ismail, Jawed A Mallick, Dahui Qin, Mohammad O Hussaini
AIM: To report the first case of a Richter syndrome where small lymphocytic lymphoma (SLL) progressed to a CD3+ diffuse large B-cell lymphoma (DLBCL). METHODS: Macrodissection of small and large cell lymphomatous components was performed. This was followed by flow cytometric analysis along with molecular B-cell immunoglobulin (heavy and light chains) and T-cell receptor (γ and β chains) gene rearrangement studies to investigate a clonal relationship between the components...
November 29, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27895166/t-cell-rich-lymphoid-infiltrates-with-large-b-cells-a-review-of-key-entities-and-diagnostic-approach
#2
REVIEW
Chee Leong Cheng, Simon O'Connor
Accurate diagnostic interpretation of a lymphoid population composed predominantly of small T cells, together with smaller numbers of large B cells, with or without a nodular architecture, is a common problem faced by the histopathologist. The differential diagnosis of this histological pattern is wide, ranging from reactive conditions such as drug reactions and viral infections, through borderline entities such as immunodeficiency-related lymphoproliferative disorders to lymphomas. The latter includes entities where the large B cells are primarily neoplastic (classical and nodular lymphocyte-predominant Hodgkin lymphomas and T cell/histiocyte-rich large B cell lymphoma) as well as T cell lymphomas such as angioimmunoblastic T cell lymphoma where the large B cells represent an epiphenomenon and may or may not be neoplastic...
November 28, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27881473/clinicopathological-features-of-neoplasms-with-neuroendocrine-differentiation-occurring-in-the-liver
#3
Yoriko Nomura, Osamu Nakashima, Jun Akiba, Sachiko Ogasawara, Shogo Fukutomi, Rin Yamaguchi, Hironori Kusano, Masayoshi Kage, Koji Okuda, Hirohisa Yano
BACKGROUND/AIMS: We investigated the clinicopathological features of hepatic neuroendocrine tumours (NET) and neuroendocrine carcinoma (NEC), which remain largely unknown. MATERIAL AND METHODS: We examined 1235 tumours from 1048 patients who had undergone curative hepatectomy for liver neoplasms at Kurume University Hospital. Pathological diagnoses were based on the 2010 WHO Classification of Tumours of the Digestive System. We performed immunostaining for hepatocyte markers (eg, hepatocyte paraffin (HepPar)-1), neuroendocrine markers (eg, chromogranin A (CGA)) and the proliferation marker (Ki-67)...
November 23, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27879345/the-role-of-lymph-node-size-and-foxp3-regulatory-t-cells-in-node-negative-colon-cancer
#4
Bruno Märkl, Beate Paul, Tina Schaller, Hallie Kretsinger, Bernadette Kriening, Gerhard Schenkirsch
Recently, we demonstrated that the intratumoural density of CD3+ and CD8+ T cells is independently prognostic and associated with lymph node (LN) harvest and LN size in node-negative colon cancer. We assumed that FOXP3+ T cells (Tregs) could be inversely associated with these LN features. Therefore, we performed a retrospective immunohistochemical analysis using an already well-characterised collection of stage I/II colon cancer cases. Receiver operating characteristic analysis revealed the optimal cut-off for predicting cancer-related death to be 70 FOXP3+ Tregs/mm(2) at the invasion front...
November 22, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27872140/primary-cilia-are-increased-in-number-and-demonstrate-structural-abnormalities-in-human-cancer
#5
Binnaz Yasar, Kim Linton, Christian Slater, Richard Byers
AIMS: Primary cilia play an important role in the regulation of cell signalling pathways and are thought to have a role in cancer but have seldom been studied in human cancer samples. METHODS: Primary cilia were visualised by dual immunofluorescence for anti-CROCC (ciliary rootlet coiled-coil) and anti-tubulin in a range of human cancers (including carcinomas of stomach, pancreas, prostate, lung and colon, lobular and ductal breast cancers and follicular lymphoma) and in matched normal tissue (stomach, pancreas, lung, large and small intestines, breast and reactive lymph nodes) samples using a tissue microarray; their frequency, association with proliferation, was measured by Ki-67 staining and their structure was analysed...
November 21, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27872139/microsatellite-instability-evaluation-by-automated-microfluidic-electrophoresis-an-update
#6
LETTER
Francesco Pepe, Riccardo Smeraglio, Davide Vacirca, Umberto Malapelle, Massimo Barberis, Giancarlo Troncone
No abstract text is available yet for this article.
November 21, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27864452/identifying-progression-predictors-of-breast-ductal-carcinoma-in-situ
#7
REVIEW
Joe Yeong, Aye Aye Thike, Puay Hoon Tan, Jabed Iqbal
Ductal carcinoma in situ (DCIS) refers to neoplastic epithelial cells proliferating within the mammary ducts of the breast, which have not breached the basement membrane nor invaded surrounding tissues. Traditional thinking holds that DCIS represents an early step in a linear progression towards invasive ductal carcinoma (IDC). However, as only approximately half of DCIS cases progress to IDC, important questions around the key determinants of malignant progression need to be answered. Recent studies have revealed that molecular differences between DCIS and IDC cells are not found at the genomic level; instead, altered patterns of gene expression and post-translational regulation lead to distinct transcriptomic and proteomic profiles...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27864451/reacquisition-of-e-cadherin-expression-in-metastatic-deposits-of-signet-ring-cell-carcinoma-of-the-upper-gastrointestinal-system-a-potential-anchor-for-metastatic-deposition
#8
Yihong R Ma, Gene P Siegal, Shi Wei
AIMS: To examine the expression of E-cadherin in paired primary and metastatic signet-ring cell carcinomas (SRCC) of various organ systems in order to explore the potential role of the molecule in metastatic dissemination of this unique tumour type. METHODS: Thirty-seven consecutive cases of SRCC from various organs with paired primary and metastatic tumorous tissue available were retrieved. The intensity of membranous E-cadherin expression was semiquantitatively scored on a scale of 0-3+...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27864450/mage-a-family-expression-is-correlated-with-poor-survival-of-patients-with-lung-adenocarcinoma-a-retrospective-clinical-study-based-on-tissue-microarray
#9
Meixiang Sang, Lina Gu, Danjing Yin, Fei Liu, Yishui Lian, Xiaochong Zhang, Shina Liu, Weina Huang, Yunyan Wu, Baoen Shan
OBJECTIVES: As the best characterised cancer/testis antigen family members, melanoma-associated antigens (MAGE) have been reported to be expressed in various malignant tumours. However, the expression pattern of MAGE-A family in lung adenocarcinoma (LAC) specimens and their prognostic and therapeutic significance for patients with LAC is still unclear. MATERIALS AND METHODS: Tissue microarray-based immunohistochemistry analysis was used to examine the expression of MAGE-A family members (including MAGE-A1, A2, A3, A4, A6, A10, A11 and A12) in 105 paired LAC specimens and the corresponding pericarcinoma specimens...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27864449/what-are-the-critical-steps-in-processing-blood-cultures-a-prospective-audit-evaluating-current-practice-of-reporting-blood-cultures-in-a-centralised-laboratory-serving-secondary-care-hospitals
#10
Manjula Meda, James Clayton, Reela Varghese, Jayakeerthi Rangaiah, Clive Grundy, Farnaz Dashti, David Garner, Katherine Groves, Karen Fitzmaurice, E Hutley
AIMS: To assess current procedures of processing positive blood cultures against national standards with an aim to evaluate its clinical impact and to determine the utility of currently available rapid identification and susceptibility tests in processing of blood cultures. METHODS: Blood cultures from three secondary care hospitals, processed at a centralised laboratory, were prospectively audited. Data regarding processing times, communication with prescribers, changes to patient management and mortality within 30 days of a significant blood culture were collected in a preplanned pro forma for a 4-week period...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27864448/evaluation-of-a-rapid-antigen-test-for-detection-of-streptococcus-pneumoniae-in-cerebrospinal-fluid
#11
Angel Boulos, Derek Fairley, James McKenna, Peter Coyle
Detection of Streptococcus pneumoniae antigen in cerebrospinal fluid (CSF) using lateral flow immunochromatography tests (ICTs) is an effective, rapid and low-cost method to diagnose pneumococcal meningitis. This study evaluated the diagnostic accuracy of the Uni-Gold ICT to detect pneumococcal antigen in CSF specimens, compared with gold standard bacteriology and quantitative real-time PCR (qPCR) testing. CSF specimens (n=69) from patients with suspected bacterial meningitis were included in the study. 13/69 (19%) were positive and 56/69 (81%) were negative for pneumococcus by the gold standard tests...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27856679/a-simple-human-co-culture-model-of-lymphatic-invasion
#12
Kalnisha Naidoo
True lymphovascular invasion in most carcinomas adversely affects survival, but in vitro human models of lymphatic invasion are lacking. Herein is discussed one such model in which certain mechanistic aspects of lymphatic dissemination can be investigated easily in any laboratory. S100P expression is known to be significantly upregulated in the lymph node metastases of human pancreatic adenocarcinoma. Thus, an S100P overexpressing cell line (S5L) and a vector control (V3L) pancreatic cancer cell lines were used to develop a co-culture model with human dermal lymphatic endothelial cells...
November 17, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27856678/a-rare-and-unusual-cause-of-mammographic-calcification-in-the-breast
#13
Andrew Robinson, Abeer M Shaaban
No abstract text is available yet for this article.
November 17, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27852687/incidental-ebv-positivity-in-paediatric-post-transplant-specimens-demonstrates-the-need-for-stringent-criteria-for-diagnosing-post-transplant-lymphoproliferative-disorders
#14
Rebecca L King, Michele E Paessler, Matthew T Howard, Gerald B Wertheim
AIMS: To examine the need for minimal diagnostic criteria for post-transplant lymphoproliferative disorders (PTLD) in children, we sought to determine the rate of incidental Epstein-Barr virus (EBV)-positivity in tissues from organ transplant recipients (OTR). METHODS: EBV in situ hybridisation (ISH) was done retrospectively on tissue from 34 paediatric autopsies of OTR and paediatric tonsillectomy specimens from non-OTR (96) and OTR (6). Patients with a history of PTLD were excluded from both data sets...
November 15, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27849157/success-in-adrenal-venous-sampling-between-two-protocols-experience-at-a-tertiary-centre
#15
LETTER
Mohammad Ali Husainy, Cheng Fang, Ana Nicolescu, Dylan Lewis, Pauline Kane, Royce P Vincent
No abstract text is available yet for this article.
November 14, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27849156/molecular-characterisation-of-acute-intermittent-porphyria-in-a-cohort-of-south-african-patients-and-kinetic-analysis-of-two-expressed-mutants
#16
Philip Fortgens, Elaine Pienaar, Anne Corrigall, Mark Sonderup, C Wendy Spearman, Peter Meissner
AIMS: Acute intermittent porphyria (AIP) is a disorder of the haem biosynthetic pathway caused by mutations in the hydroxymethylbilane synthase (HMBS) gene. Knowledge of the spectrum of mutations present in South Africa is limited. This study presents the molecular profile of 20 South African patients with AIP, and the kinetic analysis of one novel expressed mutated HMBS enzyme and a previously identified mutation at the same position. METHODS: Genomic DNA was isolated from affected probands and selected family members, the HMBS gene amplified and mutations characterised by direct sequencing and restriction enzyme analysis...
November 14, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27836923/single-nucleotide-polymorphism-array-snp-a-improves-the-identification-of-chromosomal-abnormalities-by-metaphase-cytogenetics-in-myelodysplastic-syndrome
#17
Fernanda Borges da Silva, João Agostinho Machado-Neto, Virginia Helena Leira Lipoli Bertini, Elvira Deolinda Rodrigues Pereira Velloso, Cristina Alonso Ratis, Rodrigo T Calado, Belinda Pinto Simões, Eduardo Magalhães Rego, Fabiola Traina
AIMS: The myelodysplastic syndromes (MDS) are a heterogeneous group of clonal haematopoietic stem cell disorders characterised by inefficient haematopoiesis and risk of progression to acute myeloid leukaemia. Metaphase cytogenetics is an extremely valuable clinical tool in the management of haematological malignancies. However, metaphase cytogenetics requires cellular proliferation, its sensitivity and resolution depends on the proportion of clonal cells in the sample and size of the lesion, respectively...
November 11, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27802414/solitary-fibrous-tumour-of-the-genitourinary-tract-a-clinicopathological-study-of-11-cases-and-their-association-with-the-nab2-stat6-fusion-gene
#18
Erik Kouba, Novae B Simper, Shaoxiong Chen, Sean R Williamson, David J Grignon, John N Eble, Gregory T MacLennan, Rodolfo Montironi, Antonio Lopez-Beltran, Adeboye O Osunkoya, Shaobo Zhang, Mingsheng Wang, Lisha Wang, Thu Tran, Robert E Emerson, Lee Ann Baldrige, M Francesca Monn, Konstantinos Linos, Liang Cheng
AIMS: To characterise clinicopathological features and clinical outcomes of the genitourinary tract solitary fibrous tumours, incorporating NAB2-STAT6 gene fusion status. METHODS: The presence of the molecular hallmark NAB2-STAT6 gene fusion and for the defining fusion partner product STAT6 was assessed in 11 cases of the genitourinary tract solitary fibrous tumours. NAB2-STAT6 gene fusion analysis was performed using a break-apart fluorescence in situ hybridisation (FISH) probe using a probe cocktail with Bacterial artificial chromosome (BAC) clones for STAT6 and NAB2...
October 31, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27802413/validation-and-verification-of-automated-urine-particle-analysers
#19
REVIEW
Giuseppe Enrico Bignardi
There is often uncertainty on how validation and verification of newly introduced tests should be conducted, and there is a real risk of verification becoming a meaningless ritual, rather than a useful exercise. This article reviews the literature and makes recommendations regarding the validation and verification of automated urine particles analysers. A generic practical approach to verification is also recommended. For many analysers, the accuracy of white blood cells, epithelial cells and bacterial counts is corroborated by a number of independent evaluations; thus, any verification laboratory work could be significantly scaled down...
October 31, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27802412/cytographic-changes-on-bc-6800-haematological-analyzer-related-to-the-presence-of-candida-albicans-in-peripheral-blood-a-new-tool-to-suspect-candidemia
#20
Antonio La Gioia, Alessandra Devito, Fabiana Fiorini, Maria Bombara, Patrizia Isola, Barbara Spinale, Lucia Francioni, Domenico Salamone, Paola Marelli, Sabrina Buoro, Marcello Fiorini
BACKGROUND: We studied the quantitative and cytographic changes that the presence of Candida albicans (C. albicans) in peripheral blood (PB) samples causes on the Mindray BC-6800 Haematological Analyzer. METHODS: A simulated in vitro candidemia was obtained by adding a different amount of C. albicans to discarded remnants of PB samples. Quantitative data and cytographic features were evaluated immediately as well as after 120 and 240 min of the yeast addition...
October 31, 2016: Journal of Clinical Pathology
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