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Journal of Clinical Pathology

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https://www.readbyqxmd.com/read/28325747/brief-fixation-enables-same-day-breast-cancer-diagnosis-with-reliable-assessment-of-hormone-receptors-e-cadherin-and-her2-neu
#1
Altuna Halilovic, Joris Bulte, Yvonne Jacobs, Hanneke Braam, Patricia van Cleef, Margrethe Schlooz-Vries, Annelies Werner, Oliver Boelens, Iris Nagtegaal, Hans de Wilt, Peter Bult
AIMS: Preoperative core needle biopsy (CNB) is commonly used to confirm the diagnosis of breast cancer. For treatment purposes and for determining histological type, especially in case of neoadjuvant therapy, oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) status and E-cadherin assessments are crucial. Considering the increasing demand for same-day diagnosis of breast lesions, an accelerated method of CNB processing was developed, in which the tissue fixation time is radically reduced...
March 21, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28314739/intratumorous-heterogeneity-for-ras-mutations-in-a-treatment-na%C3%A3-ve-colorectal-tumour
#2
Sebastian Lunke, Belinda Lee, Sevastjan Kranz, Peter Gibbs, Paul Waring, Michael Christie
Activating mutations in KRAS and NRAS genes in patients with colorectal cancer (CRC) are associated with a lack of response to treatment with anti-epidermal growth factor receptor (EGFR) therapies. Mutations in these genes are thought to be mutually exclusive, however reports have described CRCs with two activating rat sarcoma (RAS) mutations. This has fuelled discussion about whether these mutations are the result of intratumorous heterogeneity, or if they are co-occurring in the same cancer cell clone. We present a case of a colorectal tumour with three RAS mutations detected during routine diagnostic testing...
March 17, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28298390/clinical-usefulness-of-the-free-web-based-image-analysis-application-immunoratio-for-assessment-of-ki-67-labelling-index-in-breast-cancer
#3
Min-Kyung Yeo, Hee Eun Kim, Sung Hun Kim, Byung Joo Chae, Byung Joo Song, Ahwon Lee
AIMS: Ki-67 is a prognostic marker in breast cancer; however, the use of the Ki-67 labelling index (LI) in clinical practice requires a consistent and easily accessible scoring method. The present study evaluated the use of the free internet-based image analysis program ImmunoRatio to score Ki-67 LI in breast cancer in comparison with manual counting. METHODS: Ki-67 immunohistochemical detection was performed in 577 breast cancer cases, and the Ki-67 LI was determined by ImmunoRatio and manual counting...
March 15, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28292978/validating-a-fully-automated-real-time-pcr-based-system-for-use-in-the-molecular-diagnostic-analysis-of-colorectal-carcinoma-a-comparison-with-ngs-and-ihc
#4
Richard Colling, Lai Mun Wang, Elizabeth Soilleux
BACKGROUND: Molecular testing is increasingly needed in colorectal carcinoma (CRC) and the current clinically relevant mutations are in BRAF, KRAS and NRAS. This study aimed to further validate a new alternative polymerase chain reaction (PCR) platform (Idylla, Biocartis) against existing next-generation sequencing (NGS) and immunohistochemistry (IHC) assays. METHODS: 56 Idylla tests were performed on 43 CRC cases, in a total of 74 comparisons against an NGS panel (Ion Torrent) and the VE1 (anti-BRAF) antibody IHC...
March 14, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28289065/gleason-grade-grouping-of-prostate-cancer-is-of-prognostic-value-in-asian-men
#5
Joe Yeong, Rehena Sultana, Jonathan Teo, Hong Hong Huang, John Yuen, Puay Hoon Tan, Li Yan Khor
AIM: The International Society of Urological Pathology made recommendations for the use of Grade Groups (GG) originally described by Epstein and colleagues over Gleason score (GS) alone in 2014, which was subsequently adopted by the WHO classification in 2016. The majority of studies validating this revision have been in Caucasian populations. We therefore asked whether the new GG system was retrospectively associated with biochemical disease-free survival in a mixed-ethnicity cohort of Asian men...
March 13, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28275044/variation-in-haemoglobin-measurement-across-different-hemocue-devices-and-device-operators-in-rural-cambodia
#6
Aviva I Rappaport, Susan I Barr, Timothy J Green, Crystal D Karakochuk
: Point-of-use haemoglobinometers, such as the HemoCue, are a common method to measure haemoglobin (Hb) concentration in field settings as the device is portable, requires only a small finger-prick capillary blood sample and computes an immediate Hb reading. The aim of this study was to compare Hb measurements across different HemoCue devices and across device operators using capillary blood samples collected from women during a trial in rural Cambodia. We compared mean±SD capillary Hb concentration (g/L) across n=12 different HemoCue Hb 301 devices and across n=9 device operators among 2846 Cambodian women...
March 8, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28264878/assessment-of-alk-gene-rearrangement-in-lung-cancer-using-a-new-rapid-automated-surefish-dako-omnis-assay
#7
Maiko Takeda, Takahiko Kasai, Shigeki Shimizu, Masanori Kitaichi, Kensuke Kojima, Akihiro Nagoya, Akio Hayashi, Tomoki Utsumi, Hyung-Eun Yoon, Akihide Matsumura, Shinji Atagi
The ALK fluorescence in situ hybridisation (FISH) method is the examination essential for pathological diagnosis and choice of molecular-targeted therapy in ALK-rearranged lung cancer. Here, for detection of ALK gene rearrangement in patients with lung cancer, we evaluated the rapid FISH technology (ALK SureFISH), a newly developed assay for the automated staining platform Dako Omnis, using 21 formalin-fixed paraffin-embedded (FFPE) samples. All cases could be evaluated with the SureFISH method. SureFISH provided excellent quality signals without any background staining...
March 6, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28258096/extramural-venous-invasion-in-colorectal-cancer-stage-i
#8
LETTER
Klaus Dirschmid, William Sterlacci, Michaela Rhomberg, Felix A Offner
No abstract text is available yet for this article.
March 3, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28249942/quantifying-tumour-vascularity-in-non-luminal-breast-cancers
#9
Maria Ryssdal Kraby, Signe Opdahl, Lars A Akslen, Anna M Bofin
AIMS: Microvessel density (MVD), proliferating MVD (pMVD) and vascular proliferation index (VPI) are methods used to quantify tumour vascularity in histopathological sections. In this study, we assessed MVD, pMVD and VPI in non-luminal subtypes of breast cancer. Differences between subtypes were studied, and the prognostic value of each method was assessed. METHODS: All non-luminal subtypes (61 basal phenotype (BP), 60 human epidermal growth factor receptor 2 (HER2) type and 30 five negative phenotype (5NP)) were selected from a series comprising 909 cases of breast cancer...
March 1, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28249941/plasmablastic-lymphoma-versus-plasmablastic-myeloma-an-ongoing-diagnostic-dilemma
#10
Janice S Ahn, Ryan Okal, Jeffrey A Vos, Matthew Smolkin, Abraham S Kanate, Flavia G Rosado
AIMS: To determine the utility of clinical, morphological and phenotypical features in the differential diagnosis of plasmablastic lymphoma and myeloma with plasmablastic features. METHODS: All plasmablastic neoplasms identified from a 15-year retrospective search were reviewed and classified into 'lymphoma', 'myeloma' or 'indeterminate'. The classification was then compared with the previously established clinical diagnosis. Lessons learned from this review were used to design a diagnostic algorithm for pathologists to use in the absence of known clinical history...
March 1, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28242622/hbme1-expression-in-warthin-tumour-relationships-to-ck5-6-p63-bcl2-and-wt1-expression-and-histogenesis
#11
LETTER
Adriana Handra-Luca
No abstract text is available yet for this article.
February 27, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28213561/primary-care-requests-for-anaemia-chemistry-tests-in-spain-potential-iron-transferrin-and-folate-over-requesting
#12
Maria Salinas, Maite López-Garrigós, Emilio Flores, Carlos Leiva-Salinas
AIM: To study the regional variability of requests for anaemia chemistry tests in primary care in Spain and the associated economic costs of potential over-requesting. METHODS: Requests for anaemia tests were examined in a cross-sectional study. Clinical laboratories from different autonomous communities (AACCs) were invited to report on primary care anaemia chemistry tests requested during 2014. Demand for iron, ferritin, vitamin B12 and folate tests per 1000 inhabitants and the ratios of the folate/vitamin B12 and transferrin/ferritin requests were compared between AACCs...
February 17, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28209662/the-impact-of-between-analytical-platform-variability-on-the-classification-of-pleural-effusions-into-exudate-or-transudate-using-light-s-criteria
#13
Michael P Cornes, Andrew J Chadburn, Claire Thomas, Catherine Darby, Rachel Webster, Clare Ford, Rousseau Gama
BACKGROUND: Light's criteria are ratios of pleural fluid to serum total protein (TP), pleural fluid to serum lactate dehydrogenase (LDH) and pleural fluid LDH to the upper reference limit for serum LDH. They are used to classify pleural effusions into an exudate or transudate when pleural fluid protein is 25-35 g/L. We evaluated the impact of between analytical platforms on the classification of pleural effusions using Light's criteria. METHODS: Light's criteria were used to classify pleural effusions with fluid TP between 25 and 35 g/L into exudate and transudate...
February 16, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28202495/expression-of-grk2-and-igf1r-in-hepatocellular-carcinoma-clinicopathological-and-prognostic-significance
#14
Song-Bai Lin, Li Zhou, Zhi-Yong Liang, Wei-Xun Zhou, Ye Jin
AIM: It has been shown that G-protein-coupled receptor kinase 2 (GRK2) negatively regulates the insulin-like growth factor 1 receptor (IGF1R) signalling pathway in hepatocellular carcinoma (HCC). The aim of this study was to evaluate the clinicopathological and prognostic significance of GRK2 and IGF1R in HCC. METHODS: Expression of GRK2 and IGF1R was first detected by tissue microarray-based immunohistochemistry in 156 patients with HCC. Staining results, termed the H-score, were then correlated with clinicopathological variables and patient survival...
February 15, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28183782/telomere-maintenance-in-soft-tissue-sarcomas
#15
REVIEW
Nicholas Eastley, Barbara Ottolini, Carmen Garrido, Jacqueline A Shaw, Thomas Alasdair McCulloch, Robert U Ashford, Nicola J Royle
Soft tissue sarcomas (STS) are a diverse group of heterogeneous malignant tumours derived from mesenchymal tissues. Over 50 different STS subtypes are recognised by WHO, which show a wide range of different biological behaviours and prognoses. At present, clinicians managing this complex group of tumours face several challenges. This is reflected by the relatively poor outcome of patients with STSs compared with many other solid malignant tumours. These include difficulties securing accurate diagnoses, a lack of effective systemic treatments and absence of any sensitive circulating biomarkers to monitor patients throughout their treatment and follow-up...
February 9, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28183781/aligning-the-flow-cytometric-evaluation-with-the-diagnostic-need-an-evidence-based-approach
#16
Dawn Williams-Voorbeijpel, Francisco Sanchez, Christine G Roth
AIMS: Elimination of non-value added testing without compromising high-quality clinical care is an important mandate for laboratories in a value-based reimbursement system. The goal of this study was to determine the optimal combination of flow cytometric markers for a screening approach that balances efficiency and accuracy. METHODS: An audit over 9 months of flow cytometric testing was performed, including rereview of all dot plots from positive cases. RESULTS: Of the 807 cases in which leukaemia/lymphoma testing was performed, 23 were non-diagnostic and 189 represented bronchoalveolar lavage specimens...
February 9, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28167490/a-snake-in-the-grass
#17
Rhiannon Trefor, Murali Varma
A 77-year-old man presented with haematuria. Cystoscopy revealed a papillary tumour in the trigone region of the bladder. A TURBT was performed. Review the high-quality, interactive digital Aperio slide at http://virtualacp.com/JCPCases/jclinpath-2016-204015 and consider your diagnosis. WHAT IS YOUR DIAGNOSIS?: Urothelial carcinoma pTaUrothelial carcinoma pT1Metastatic carcinomaProstatic adenocarcinomaProstatic polyp.
February 6, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28159767/ticagrelor-reversal-in-vitro-assessment-of-four-haemostatic-agents
#18
Leyla Calmette, Anne-Céline Martin, Bernard Le Bonniec, Diane Zlotnik, Isabelle Gouin-Thibault, Christilla Bachelot-Loza, Pascale Gaussem, Anne Godier
AIM: Management of ticagrelor-induced bleeding is challenging as platelet transfusion is ineffective. An effective strategy is needed. This study aimed to investigate in vitro the efficacy of four haemostatic drugs (HDs), namely recombinant activated factor VII (rFVIIa), fibrinogen concentrate (Fib), tranexamic acid (TXA) and factor XIII concentrate (FXIII) to improve the haemostatic capacity in the presence of ticagrelor. METHODS: Blood was spiked with ticagrelor then supplemented by either HD or control...
February 3, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28153953/evaluation-of-a-fast-and-fully-automated-platform-to-diagnose-egfr-and-kras-mutations-in-formalin-fixed-and-paraffin-embedded-non-small-cell-lung-cancer-samples-in-less-than-one-day
#19
Laetitia Lambros, Charline Caumont, Briac Guibourg, Fanny Barel, Isabelle Quintin-Roué, Pascale Marcorelles, Jean-Philippe Merlio, Arnaud Uguen
AIMS: Searching for EGFR and KRAS mutations within non-small cell lung carcinoma (NSCLC) samples remains time-consuming and can delay treatment choices in patients with acute deterioration. We evaluated the performances of the fully automated Idylla platform to quickly detect these mutations in NSCLC samples. METHODS: We used the Idylla EGFR Mutation Assay and the Idylla KRAS Mutation Test to analyse 18 formalin-fixed paraffin-embedded NSCLC tumour samples with known EGFR and KRAS mutation status according to next-generation sequencing (NGS) and droplet digital PCR (ddPCR) for EGFRT790M mutations...
February 2, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28153952/qmc-pcrx-a-novel-method-for-rapid-mutation-detection
#20
Henry O Ebili, James Hassall, Abutaleb Asiri, Hersh Ham-Karim, Wakkas Fadhil, Ayodeji Johnson Agboola, Mohammad Ilyas
AIMS: We previously described the quick multiplex consensus PCR (QMC-PCR) as a method for rapid mutation screening in low-quality template. QMC-PCR has two-stages: a prediagnostic multiplex (PDM) reaction followed by a single specific diagnostic reaction with high-resolution melting (HRM) analysis. We aimed to develop QMC-PCRx in which second stage was multiplexed to allow testing of multiple targets. METHODS: The PDM reaction was retained without change. For the second stage, in silico design was used to identify targets amenable to a multiplex specific diagnostic reaction and multiplex HRM (mHRM) analysis...
February 2, 2017: Journal of Clinical Pathology
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