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Journal of Biochemistry

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https://www.readbyqxmd.com/read/28204531/crystal-structure-of-metagenomic-%C3%AE-xylosidase-%C3%AE-l-arabinofuranosidase-activated-by-calcium
#1
Tomohiko Matsuzawa, Satoshi Kaneko, Naomi Kishine, Zui Fujimoto, Katsuro Yaoi
No abstract text is available yet for this article.
February 16, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28201527/high-dose-of-antibiotic-colistin-induces-oligomerization-of-molecular-chaperone-hsp90
#2
Shuntaro Togashi, Kyosuke Takahashi, Arisa Tamura, Ikumi Toyota, Shiori Hatakeyama, Atsushi Komatsuda, Ikuru Kudo, Erina Sasaki Kudoh, Tomoya Okamoto, Asami Haga, Asuka Miyamoto, Ewa Grave, Taku Sugawara, Hiroaki Shimizu, Hideaki Itoh
No abstract text is available yet for this article.
February 14, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28169401/giardia-duodenalis-rad52-protein-biochemical-characterization-and-response-upon-dna-damage
#3
Rosa María Martínez Miguel, Antonio Sandoval Cabrera, María Luisa Bazán-Tejeda, Ana Laura Torres Huerta, Diego A Martínez Reyes, Rosa María Bermúdez Cruz
No abstract text is available yet for this article.
February 7, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28158808/simultaneous-ablation-of-prmt-1-and-prmt-5-abolishes-asymmetric-and-symmetric-arginine-dimethylations-in-caenorhabditis-elegans
#4
Keiko Hirota, Chihiro Shigekawa, Sho Araoi, Liang Sha, Takayuki Inagawa, Akihiko Kanou, Koichiro Kako, Hiroaki Daitoku, Akiyoshi Fukamizu
No abstract text is available yet for this article.
February 2, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28158602/plakoglobin-maintains-the-integrity-of-vascular-endothelial-cell-junctions-and-regulates-vegf-induced-phosphorylation-of-ve-cadherin
#5
Fumitaka Muramatsu, Hiroyasu Kidoya, Hisamichi Naito, Yumiko Hayashi, Tomohiro Iba, Nobuyuki Takakura
No abstract text is available yet for this article.
February 2, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130419/the-retinoic-acid-receptor-related-orphan-receptor-%C3%AE-positively-regulates-tight-junction-protein-claudin-domain-containing-1-mrna-expression-in-human-brain-endothelial-cells
#6
Hiroshi Matsuoka, Akiho Shima, Arisa Uda, Hirotaka Ezaki, Akihiro Michihara
Members of the claudin family play important roles in the formation of tight junctions (TJs) in several tissues. Claudin domain containing 1 (CLDND1) is homologous to this family and localizes to TJs and the cytoplasm when exogenously expressed in cultured epithelial cell lines. Furthermore, serum antibody levels of CLDND1-derived peptides are elevated in patients with cerebral infection, cardiovascular disease, or diabetes mellitus as compared to healthy controls. However, CLDND1 transcriptional regulation remains poorly analyzed and most regional transcription factor binding sites remain to be defined...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130418/papain-cleavage-of-the-38-000-dalton-fragment-inhibits-the-binding-of-4-4-diisothiocyanostilbene-2-2-disulfonate-to-lys-539-on-the-60-000-dalton-fragment-in-human-band-3
#7
Takeo Yamaguchi, Hideaki Kojima, Shiori Kawaguchi, Maiko Shimada, Haruka Aso
Human band 3 is a 98-kDa transmembrane (TM) protein comprising 14 TM segments. Papain cleavages band 3 into 38- and 60-kDa fragments. Under vigorous conditions, the cleavage of the loop region between the TM 7 of gate domain and the TM 8 of core domain in the 38-kDa fragment produces 7- and 31-kDa fragments. Conformational changes of the TM 5 segment containing Lys-539 by cleavage of the 38-kDa fragment remain unclear. Pressure-induced hemolysis of erythrocytes was suppressed by binding of 4, 4'-diisothiocyanostilbene-2, 2'-disulfonate (DIDS) to Lys-539...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130417/phosphorylation-of-clustered-serine-residues-in-the-n-terminus-of-bps-domain-negatively-regulates-formation-of-the-complex-between-human-grb14-and-insulin-receptor
#8
Junichi Taira, Yutaka Kida, Kohei Inatomi, Hideyuki Komatsu, Yuichiro Higashimoto, Hiroshi Sakamoto
Growth factor receptor-bound protein 14 (Grb14) is a negative regulator of insulin receptor (IR) and is involved in a negative feedback mechanism of insulin signaling. Grb14 associates with IR and inhibits its tyrosine kinase activity through the between pleckstrin homology and Src homology-2 (BPS) domain. We previously reported that the pharmacological inhibition and knockdown of glycogen synthase kinase-3 (GSK-3) facilitates the insulin-induced complex formation of human Grb14 (hGrb14) and IR, suggesting that GSK-3 suppresses hGrb14 recruitment to IR...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130416/crystal-structure-of-the-solute-binding-protein-bxle-from-streptomyces-thermoviolaceus-opc-520-complexed-with-xylobiose
#9
REVIEW
Koji Tomoo, Yasuhiro Miki, Hideaki Morioka, Kiho Seike, Toshimasa Ishida, Sadao Ikenishi, Katsushiro Miyamoto, Tomokazu Hasegawa, Akihito Yamano, Kensaku Hamada, Hiroshi Tsujibo
BxlE from Streptomyces thermoviolaceus OPC-520 is a xylo-oligosaccharide (mainly xylobiose)-binding protein that serves as the initial receptor for the bacterial ABC-type xylo-oligosaccharide transport system. To determine the ligand-binding mechanism of BxlE, X-ray structures of ligand-free (open form) and ligand (xylobiose)-bound (closed form) BxlE were determined at 1.85 Å resolution. BxlE consists of two globular domains that are linked by two β-strands, with the cleft at the interface of the two domains creating the ligand-binding pocket...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130415/neu1-sialidase-controls-gene-expression-and-secretion-of-il-6-and-mcp-1through-nf-%C3%AE%C2%BAb-pathway-in-3t3-l1-adipocytes
#10
Yujin Natori, Miwako Nasui, Kiyoto Edo, Shogo Sato, Takuya Sakurai, Takako Kizaki, Fumiko Kihara-Negishi
A sialidase NEU1 that removes sialic acids from glycoconjugates has been implicated in diverse cellular functions. Aberrant NEU1 activity is associated with various pathologies including lysosomal storage disorder (LSD) sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells. We recently reported that NEU1 activity increases during 3T3-L1 adipogenesis and that it is higher in the epididymal fat of obese and diabetic mice. However, the precise functions of NEU1 in adipocytes have not been elucidated...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130414/regulation-and-physiological-functions-of-mammalian-phospholipase-c
#11
REVIEW
Yoshikazu Nakamura, Kiyoko Fukami
PHOSPHOLIPASE C PLC IS A KEY ENZYME IN PHOSPHOINOSITIDE METABOLISM PLC HYDROLYSES PHOSPHATIDYLINOSITOL 4,5-BIS-PHOSPHATE TO GENERATE 2 SECOND MESSENGERS, INOSITOL 1,4,5-TRISPHOSPHATE AND DIACYLGLYCEROL, THAT GENERATE DIVERSE CELLULAR RESPONSES PLC IS ACTIVATED BY VARIOUS SIGNALLING MOLECULES, INCLUDING CA2+, HETEROMETRIC G PROTEINS, SMALL G PROTEINS, AND RECEPTOR/NON-RECEPTOR TYROSINE KINASES IN ADDITION TO THEIR ENZYMATIC ACTIVITY, SOME PLC SUBTYPES ALSO FUNCTION AS A GUANINE NUCLEOTIDE EXCHANGE FACTOR, GTPASE-ACTIVATING PROTEIN, AND ADAPTOR PROTEIN, INDEPENDENT OF THEIR LIPASE ACTIVITY THERE ARE 13 PLC ISOZYMES IN MAMMALS, AND THEY ARE CATEGORIZED INTO 6 CLASSES BASED ON STRUCTURE GENERATION AND ANALYSIS OF GENETICALLY MODIFIED MICE HAS REVEALED THE UNEXPECTEDLY DIVERSE PHYSIOLOGICAL FUNCTIONS OF PLC ISOZYMES ALTHOUGH ALL PLC ISOZYMES CATALYZE THE SAME REACTION, EACH PLC ISOZYME HAS UNIQUE PHYSIOLOGICAL FUNCTIONS THIS REVIEW FOCUSES ON THE REGULATION AND PHYSIOLOGICAL FUNCTIONS OF PLCS...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28130413/the-cysteine-rich-domain-of-tet2-binds-preferentially-to-mono-and-dimethylated-histone-h3k36
#12
Kazuyuki Yamagata, Akira Kobayashi
Missense mutations in Ten-eleven translocation 2 (TET2) gene are frequently found in leukaemia patients. Although mutations span the entire coding region, they tend to cluster in the C-terminal enzymatic domain and a cysteine-rich (CR) domain of unknown function. Herein, we found the CR domain binds chromatin preferentially at the histone H3 tail by recognising H3 lysine 36 mono- and dimethylation (H3K36me1/2). Importantly, missense mutations in the CR domain perturbed TET2 recruitment to the target locus and its enzymatic activities...
January 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28104810/the-foxp2-forkhead-domain-binds-to-a-variety-of-dna-sequences-with-different-rates-and-affinities
#13
Helen Webb, Olga Steeb, Ashleigh Blane, Lia Rotherham, Shaun Aron, Phillip Machanick, Heini Dirr, Sylvia Fanucchi
FOXP2 is a member of the P subfamily of FOX transcription factors, the DNA binding domain of which is the winged helix forkhead domain. In this work we show that the FOXP2 forkhead domain is able to bind to various DNA sequences, including a novel sequence identified in this work, with different affinities and rates as detected using surface plasmon resonance. Combining the experimental work with molecular docking, we show that high affinity sequences remain bound to the protein for longer, form a greater number of interactions with the protein and induce a greater structural change in the protein than low affinity sequences...
January 18, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28104809/gpnmb-promotes-proliferation-of-developing-eosinophils
#14
Sae Mi Hwang, Jin Hyun Kang, Bo Kyum Kim, Tae Gi Uhm, Hye Jung Kim, Hyune-Hwan Lee, Bert Binas, Il Yup Chung
Glycoprotein non-metastatic melanoma protein B (GPNMB) is a type I transmembrane protein that is expressed in a wide variety of cell types, including hematopoietic lineages. We previously demonstrated that GPNMB is one of the most highly expressed genes at an early and intermediate stage of eosinophil development. We herein examined GPNMB expression and its possible functional effect using cord blood (CB) CD34(+) hematopoietic stem cells differentiating toward eosinophils during a 24-day culture period. Western blot and confocal microscopy analyses showed that GPNMB reached its highest levels at day 12 with most GPNMB-positive cells also expressing major basic protein 1 (MBP1), an eosinophil granule protein...
January 18, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28096454/mitochondrial-function-is-required-for-extracellular-atp-induced-nlrp3-inflammasome-activation
#15
Daichi Sadatomi, Kazutaka Nakashioya, Sayaka Mamiya, Shino Honda, Yuka Kameyama, Yasuo Yamamura, Susumu Tanimura, Kohsuke Takeda
The NLRP3 inflammasome plays a critical role in the processing and release of inflammatory cytokines, such as interleukin-1β (IL-1β) and IL-18. Accumulating evidence suggests that mitochondria are common mediators of NLRP3 inflammasome activation induced by a wide range of inflammatory stimuli; however, the precise role of mitochondria is still not fully understood. Here, we show that mitochondrial function is required for extracellular ATP-induced NLRP3 inflammasome activation. Extracellular ATP induced the loss of mitochondrial membrane potential and mitochondrial fragmentation in a different manner than other stimuli in primary mouse macrophages...
January 17, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28096453/programmable-formation-of-catalytic-rna-triangles-and-squares-by-assembling-modular-rna-enzymes
#16
Hiroki Oi, Daisuke Fujita, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa
RNA is a biopolymer that is attractive for constructing nanoscale objects with complex structures. Three-dimensional (3D) structures of naturally occurring RNAs often have modular architectures. The 3D structure of a group I (GI) ribozyme from Tetrahymena has a typical modular architecture, which can be separated into two structural modules (ΔP5 and P5abc). The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through highly specific and strong assembly between ΔP5 ribozyme and P5abc RNA...
January 17, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28096452/m-ink-a-novel-tool-for-visualizing-melanosomes-and-melanocores
#17
Morié Ishida, Soujiro Marubashi, Mitsunori Fukuda
Melanosome transfer from melanocytes to surrounding keratinocytes is one of the crucial but less well characterized steps in the process of skin pigmentation. Although several markers have generally been used to detect melanosomes in melanocytes, no suitable markers for melanosomes that have been transferred into keratinocytes have ever been reported. The M-INK probe we developed and report here specifically recognizes melanocores and thus makes visualizing melanosomes that have been incorporated into keratinocytes possible even in a fluorescent field...
January 17, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28082721/roles-of-the-cytoskeleton-cell-adhesion-and-rho-signalling-in-mechanosensing-and-mechanotransduction
#18
REVIEW
Kazumasa Ohashi, Sachiko Fujiwara, Kensaku Mizuno
All cells sense and respond to various mechanical forces in and mechanical properties of their environment. To respond appropriately, cells must be able to sense the location, direction, strength and duration of these forces. Recent progress in mechanobiology has provided a better understanding of the mechanisms of mechanoresponses underlying many cellular and developmental processes. Various roles of mechanoresponses in development and tissue homeostasis have been elucidated, and many molecules involved in mechanotransduction have been identified...
January 12, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28082720/enzymatic-kinetics-of-the-quinol-peroxidase-of-an-aggressive-periodontopathic-bacterium
#19
Tasuku Abe, Taketo Kawarai, Yukihiro Takahashi, Kiyoshi Konishi
Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the present work, we have characterized recombinant QPO purified from the membrane fraction of Escherichia coli harboring a plasmid containing QPO gene. Irreversible inactivation of QPO by high concentration of H2O2 exhibited pseudo-first order kinetics. Analysis of initial-rate kinetics of QPO may suggested that enzyme catalytic mechanism is explained by a Ping Pong Bi Bi system rather than sequential systems...
January 12, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28069863/ubiquitin-recognition-by-the-proteasome
#20
REVIEW
Yasushi Saeki
The 26S proteasome is a 2.5-MDa complex responsible for the selective, ATP-dependent degradation of ubiquitylated proteins in eukaryotic cells. Substrates in hundreds cellular pathways are timely ubiquitylated and converged to the proteasome by direct recognition or by multiple shuttle factors. Engagement of substrate protein triggers conformational changes of the proteasome, which drive substrate unfolding, deubiquitylation and translocation of substrates to proteolytic sites. Recent studies have challenged the previous paradigm that Lys48-linked tetraubiquitin is a minimal degradation signal: in addition, monoubiquitylation or multiple short ubiquitylations can serve as the targeting signal for proteasomal degradation...
January 8, 2017: Journal of Biochemistry
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