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Journal of Biochemistry

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https://www.readbyqxmd.com/read/29048484/adiponectin-suppression-of-late-inflammatory-mediator-hmgb1-induced-cytokine-expression-in-raw-264-macrophage-cells
#1
Mohamed Elfeky, Takeshi Yoneshiro, Yuko Okamatsu-Ogura, Kazuhiro Kimura
High mobility group protein B1 (HMGB1) is a late inflammatory mediator released from inflammatory cells when stimulated, resulting in exaggerating septic symptoms. We recently demonstrated that full-length adiponectin, a potent anti-inflammatory adipokine, inhibits lipopolysaccharide-induced HMGB1 release. However, the effects of adiponectin on HMGB1-induced exaggerating signals currently remain unknown. This study aimed to investigate the effects of adiponectin on the pro-inflammatory function of HMGB1 in RAW264 macrophage cells...
October 18, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29040577/metabolomics-of-postprandial-plasma-alterations-a-comprehensive-japanese-study
#2
Takeo Moriya, Yoshinori Satomi, Hiroyuki Kobayashi
While endogenous metabolites in plasma can be used as clinical biomarkers, intra-day variations should be carefully considered. The postprandial effect is a large contributing factor and is dependent on regional features (e.g., meals, ethnicity). Thus, for clinical application, regional-specific postprandial baseline data are required. In this study, 10 healthy Japanese volunteers of different ages and genders ate the same meal, and blood samples were taken 30 min before and one hour after the meal challenge...
October 4, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036651/aureobasidium-pullulans-cultured-fluid-induces-il-18-production-leading-to-th1-polarization-during-influenza-a-virus-infection
#3
Daisuke Fujikura, Daisuke Muramatsu, Kochi Toyomane, Satoko Chiba, Takuji Daito, Atsushi Iwai, Takahisa Kouwaki, Masaaki Okamoto, Hideaki Higashi, Hiroshi Kida, Hiroyuki Oshiumi
Several microbial molecules with pathogen-associated molecular patterns stimulate host innate immune responses. The innate immune system plays a crucial role in activating acquired immune response via cytokine production and antigen presentation. Previous studies have shown that Aureobasidium pullulans-cultured fluid (AP-CF), which contains β-glucan, exhibits adjuvant activity and renders mice resistance to influenza A virus infection; however, the underlying mechanism remains elusive. In this study, we investigated the innate immune response to AP-CF...
October 3, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036438/a-selective-sphingosine-1-phosphate-receptor-1-agonist-sew-2871-aggravates-gastric-cancer-by-recruiting-myeloid-derived-suppressor-cells
#4
Yujing Zhou, Feng Guo
The immune status of tumor microenvironment in gastric cancer is poorly understood, which limits the development of novel strategies in this field. Sphingosine-1-phosphate (S1P) acts as an immune modulator, but the role of S1P in gastric cancer is elusive. Here, we aim to investigate S1P receptor 1 (S1P1)-mediated effect of S1P in gastric cancer. We generated a xenograft mouse model and used SEW-2871, a S1P1 specific agonist to activate S1P1 signaling. Tumor-infiltrating lymphocytes (TILs) were isolated and analyzed using flow cytometry...
October 3, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036679/human-recombinant-fab-fragment-from-combinatorial-libraries-of-a-b-cell-lymphoma-patient-recognizes-core-protein-of-chondroitin-sulfate-proteoglycan-4
#5
Yoko Egami, Yuta Narushima, Motohiro Ohshima, Akira Yoshida, Naruki Yoneta, Yasufumi Masaki, Kunihiko Itoh
CD antigens are well known as therapeutic targets of B-cell lymphoma. To isolate therapeutic antibodies that recognize novel targets other than CD antigens, we constructed a phage display combinatorial antibody Fab library from bone marrow lymphocytes of B-cell lymphoma patient. To eliminate antibodies reactive with known B-cell lymphoma antigen, non-hematopoietic and patient's sera reactive HeLaS3 cells was selected as a target of whole cell panning. Five rounds of panning against live HeLaS3 cells retrieved single Fab clone, termed AHSA (Antibody to HeLa Surface Antigen)...
September 28, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/29036583/characterization-of-moss-ent-kaurene-oxidase-cyp701b1-using-a-highly-purified-preparation
#6
Chisato Noguchi, Sho Miyazaki, Hiroshi Kawaide, Osamu Gotoh, Yuzo Yoshida, Yuri Aoyama
CYP701B1 of the moss, Physcomitrella patents, might be a unique cytochrome P450 having the ent-kaurene oxidase (KO) activity occurring in nonvascular plant. Phylogenetic analysis suggested that the gene encoding CYP701B1 was diverged from a common ancestral gene encoding KO of vascular plants. CYP701B1 expressed in Phichia yeast microsomes was purified and characterized. The purified CYP701B1 catalyzed the oxidation of ent-kaurene to ent-kaurenoic acid through three successive monooxygenations, and the rate-limiting step of this oxidation might be the initial step that forms ent-kaurenol...
September 27, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992109/integrin-%C3%AE-1-is-bound-to-galectin-1-in-human-trophoblast
#7
Žanka Bojic-Trbojevic, Milica Jovanovic Krivokuca, Ivana Stefanoska, Nikola Kolundžic, Aleksandra Vilotic, Toshihiko Kadoya, Ljiljana Vicovac
Interaction of sugar binding proteins - galectins, with glycoconjugates is considered relevant for various reproductive processes. Galectin-1 (gal-1) is a molecule involved in trophoblast cell invasion, which is accomplished through interaction with cell surface and/or extracellular matrix glycoproteins. A possibility of interaction of endogenous gal-1 and trophoblast β1 integrins, both previously shown relevant for trophoblast invasion, was investigated. Confocal microscopy showed overlap in gal-1 and β1 integrin localization at the plasma membrane of isolated cytotrophoblast, HTR-8/SVneo extravillous trophoblast cell line and JAr choriocarcinoma cells...
September 13, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992347/common-molecular-pathogenesis-of-disease-related-intrinsically-disordered-proteins-revealed-by-nmr-analysis
#8
Yoshiki Shigemitsu, Hidekazu Hiroaki
Intrinsically disordered proteins (IDPs) are either completely unstructured or contain large disordered regions in their native state; they have drawn much attention in the field of molecular pathology. Some of them substantially tend to form protein self-assemblies, such as toxic or non-toxic aggregates and fibrils, and have been postulated to relate to diseases. These disease-related IDPs include Aβ(1-42) [Alzheimer's disease (AD)], Tau (AD and tauopathy), α-synuclein (Parkinson's disease), and p53 (cancer)...
September 11, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992042/prominent-ige-binding-and-cytokine-inducing-capacities-of-a-newly-cloned-n-terminal-region-of-der-f-14-an-apolipophorin-like-house-dust-mite-allergen
#9
Kareem Gamal ElRamlawy, Takashi Fujimura, Tsunehiro Aki, Akiyasu Okada, Takayuki Suzuki, Takuya Abe, Takaharu Hayashi, Michael J Epton, Wayne R Thomas, Inas Hussein Rafeet, Diaa Beshr Al-Azhary, Kazuhisa Ono, Seiji Kawamoto
We previously characterized a 177 kDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as trigger-factor-fused proteins in Escherichia coli...
September 11, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992234/pharmacologic-management-of-diabetic-retinopathy
#10
Akiyoshi Uemura
Diabetic retinopathy (DR) is a leading cause of vision loss in working-age populations, primarily attributable to retinal vascular hyperpermeability, hypoperfusion, and neoangiogenesis. In the past decade, laser photocoagulation and surgical interventions to treat DR have been replaced by topical administrations of anti-vascular endothelial growth factor drugs and corticosteroids. Although these drugs have revolutionized clinical management of DR, their limited efficacy and adverse effects have raised an increasing demand for new drug development...
September 6, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992227/pluripotency-and-a-secretion-mechanism-of-drosophila-transglutaminase
#11
Toshio Shibata, Shun-Ichiro Kawabata
Transglutaminase (TG) catalyzes the formation of an isopeptide bond between glutamine and lysine residues and amine incorporation into specific glutamine residues. TG is conserved in all metazoans and functions both intracellularly and extracellularly. Here we review the existing knowledge of Drosophila TG with an emphasis on its pluripotency: Drosophila TG (i) plays a key role in cuticular morphogenesis, hemolymph coagulation, and entrapment against invading pathogens, (ii) suppresses the immune deficiency pathway to enable immune tolerance against commensal bacteria through the incorporation of polyamines into the NF-κB-like transcription factor Relish as well as through the protein-protein cross-linking of Relish, (iii) forms a physical matrix in the gut through cross-linking of chitin-binding proteins, and (iv) is involved in the maintenance of homeostasis in microbiota in the gut...
September 5, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992104/a-simplified-and-sensitive-method-to-identify-alzheimer-s-disease-biomarker-candidates-using-patient-derived-induced-pluripotent-stem-cells-ipscs
#12
Keiro Shirotani, Kazuya Matsuo, Sumio Ohtsuki, Takeshi Masuda, Masashi Asai, Yumiko Kutoku, Yutaka Ohsawa, Yoshihide Sunada, Takayuki Kondo, Haruhisa Inoue, Nobuhisa Iwata
We developed a simplified and sensitive method to identify Alzheimer's disease (AD) biomarker candidates by a quantitative and targeted proteomic analysis (combination of liquid chromatography tandem mass spectrometry and multiplexed-multiple reaction monitoring/selected reaction monitoring analysis) of culture media from neurons differentiated from induced pluripotent stem cells (iPSCs) established from AD patients. We found that alpha-1-acid glycoprotein (ORM1) was decreased in the culture media of AD-iPSC-derived neurons, consistent with previous observations for AD patient cerebrospinal fluid, thus validating our new strategy...
September 4, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992222/affinity-labeling-in-situ-of-the-bl12-protein-on-e-coli-70s-ribosomes-by-means-of-a-trna-dialdehyde-derivative
#13
Codjo Hountondji, Jean-Bernard Créchet, Jean-Pierre Le Caër, Véronique Lancelot, Jean A H Cognet, Soria Baouz
In this report, we have used periodate-oxidized tRNA (tRNAox) as an affinity laleling reagent to demonstrate that: (i) the bL12 protein contacts the CCA-arm of P-site bound tRNA on the E. coli 70S ribosomes; (ii) the stoichiometry of labeling is one molecule of tRNAox bound to one polypeptide chain of endogenous bL12; (iii) cross-linking in situ of bL12 with tRNAox on the ribosomes provokes the loss of activity; (iv) intact tRNA protects bL12 in the 70S ribosomes against cross-linking with tRNAox; (v) both tRNAox and pyridoxal 5'-phosphate (PLP) compete for the same or for proximal cross-linking site(s) on bL12 inside the ribosome; (vi) the stoichiometry of cross-linking of PLP to the recombinant E...
August 16, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992041/peripheral-tissue-levels-and-molecular-species-compositions-of-n-acyl-phosphatidylethanolamine-and-its-metabolites-in-mice-lacking-n-acyl-phosphatidylethanolamine-specific-phospholipase-d
#14
Manami Inoue, Kazuhito Tsuboi, Yoko Okamoto, Mayumi Hidaka, Toru Uyama, Toshihiko Tsutsumi, Tamotsu Tanaka, Natsuo Ueda, Akira Tokumura
N-acylethanolamines (NAEs), a class of lipid mediators, are produced from N-acyl-phosphatidylethanolamine (NAPE) by several pathways, including the direct release by NAPE-specific phospholipase D (NAPE-PLD) or the multistep pathway via sn-glycero-3-phospho-N-acylethanolamine (Gp-NAE). Using liquid chromatography-tandem mass spectrometry, we compared peripheral tissue levels of NAPE, Gp-NAE and NAE in NAPE-PLD-deficient (NAPE-PLD-/-) and wild type (WT) mice. NAPE-PLD was suggested to play a major role in the NAPE degradation in heart, kidney, and liver, but not in jejunum, because the NAPE levels except jejunum were significantly higher in NAPE-PLD-/- mice than in WT mice...
August 16, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28992264/proteasome-26s-subunit-psmd1-regulates-breast-cancer-cell-growth-through-p53-protein-degradation
#15
Toshiyuki Okumura, Kazuhiro Ikeda, Takafumi Ujihira, Koji Okamoto, Kuniko Horie-Inoue, Satoru Takeda, Satoshi Inoue
Endocrine therapy using antiestrogens and aromatase inhibitors is usually efficient to treat patients with hormone-sensitive breast cancer. Many patients with endocrine therapy, however, often acquire resistance. In the present study, we performed functional screening using short hairpin RNA library to dissect genes involved in antiestrogen tamoxifen resistance in MCF-7 breast cancer cells. We identified seven candidate genes that are associated with poor prognosis of breast cancer patients based on clinical dataset...
August 3, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28981752/thermodynamic-analyses-of-amino-acid-residues-at-the-interface-of-an-antibody-b2212a-and-its-antigen-roundabout-homolog-1
#16
Anna Yui, Hiroki Akiba, Shota Kudo, Makoto Nakakido, Satoru Nagatoishi, Kouhei Tsumoto
Artificial affinity maturation of antibodies is promising but often shows difficulties because the roles of each amino acid residue are not well known. To elucidate their roles in affinity against the antigen and thermal stability, interface residues in single-chain Fv of an antibody B2212A with its antigen roundabout homolog 1 were mutated and analyzed. Some amino acids played important roles in the affinity while others contributed to thermal stability.
October 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28981751/multiple-ways-to-prevent-transmission-of-paternal-mitochondrial-dna-for-maternal-inheritance-in-animals
#17
REVIEW
Ken Sato, Miyuki Sato
Mitochondria contain their own DNA (mtDNA). In most sexually reproducing organisms, mtDNA is inherited maternally (uniparentally); this type of inheritance is thus referred to as 'maternal (uniparental) inheritance'. Recent studies have revealed various mechanisms to prevent the transmission of sperm-derived paternal mtDNA to the offspring, thereby ensuring maternal inheritance of mtDNA. In the nematode Caenorhabditis elegans, paternal mitochondria and their mtDNA degenerate almost immediately after fertilization and are selectively degraded by autophagy, which is referred to as 'allophagy' (allogeneic [non-self] organelle autophagy)...
October 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28981750/prospect-of-divergent-roles-for-the-cul3-system-in-vascular-endothelial-cell-function-and-angiogenesis
#18
REVIEW
Tomohisa Sakaue, Masashi Maekawa, Hironao Nakayama, Shigeki Higashiyama
Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogenic and angiostatic responses, endothelial cells play a central role in the blood vessel formation and regression. Angiostatic responses, which are evoked by crucial factors such as VEGF and DLL4, have been elucidated...
October 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28486685/inhibition-of-ser-thr-phosphatase-ppm1d-induces-neutrophil-differentiation-in-hl-60-cells
#19
Rui Kamada, Fuki Kudoh, Fumihiko Yoshimura, Keiji Tanino, Kazuyasu Sakaguchi
Protein phosphatase Magnesium-dependent 1, Delta (PPM1D) is a wild-type p53-inducible Ser/Thr phosphatase that acts as a negative regulator of the p53 tumor suppressor. Gene amplification and overexpression of PPM1D have been reported in various cancers including leukemia and neuroblastoma. Therefore, PPM1D is a promising target in cancer therapy. It has been reported that PPM1D knockout mice exhibit neutrophilia in blood and show a defective immune response. Here, we found that inhibition of PPM1D induced neutrophil differentiation of human promyelocytic leukemia cell line HL-60...
October 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28460043/cell-free-mitochondrial-fusion-assay-detected-by-specific-protease-reaction-revealed-ca2-as-regulator-of-mitofusin-dependent-mitochondrial-fusion
#20
Naotada Ishihara, Maki Maeda, Tadato Ban, Katsuyoshi Mihara
Mitochondrial dynamic by frequent fusion and fission have important roles in various cellular signalling processes and pathophysiology in vivo. However, the molecular mechanisms that regulate mitochondrial fusion, especially in mammalian cells, are not well understood. Accordingly, we developed a novel biochemical cell-free mitochondrial fusion assay system using isolated human mitochondria. We used a protease and its specific substrate that are essential for yeast autophagy; Atg4 protease is required for maturation and the de-conjugation of the ubiquitin-like modifier Atg8...
October 1, 2017: Journal of Biochemistry
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