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Biochemistry. Biokhimii︠a︡

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https://www.readbyqxmd.com/read/27914464/immunogenicity-of-human-interferon-beta-containing-pharmaceuticals
#1
V D Nazarov, S V Lapin, A V Mazing, E P Evdoshenko, A A Totolian
Multiple sclerosis is a severe autoimmune disease with inflammatory component that continues to be resistant to treatment. One of the approaches retarding its progression is based on using nonspecific therapy with human interferon-beta (IFN-β)-containing pharmaceuticals. Neutralizing antibodies (NAbs) against genetically engineered pharmaceuticals developed by the patient's immune system, which reduce their therapeutic and biological activity, pose a serious problem. Cell lines sensitive to IFN-β activity also quantifying NAb level are applied because direct measurement of IFN-β antiviral activity is complicated...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914463/study-of-immunomodulatory-effects-of-extracellular-hsp70-in-a-mouse-model-of-allergic-airway-inflammation
#2
M A Shevchenko, N I Troyanova, E A Servuli, E L Bolkhovitina, A S Fedorina, A M Sapozhnikov
Immunostimulatory properties of extracellular heat shock proteins 70 kDa (HSP70) became interesting for investigators a long time ago. However, in recent years a series of works showing a significant relation of the immunostimulating effects of recombinant HSP70 to contamination of the protein samples with bacterial endotoxins (lipopolysaccharide, LPS) has been published. The authors showed that intensive elimination of LPS from the protein samples resulted in inversion of immunostimulating effects of HSP70 to immunosuppressive activity of the protein...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914462/recombinant-mhc-tetramers-for-isolation-of-virus-specific-cd8-cells-from-healthy-donors-potential-approach-for-cell-therapy-of-posttransplant-cytomegalovirus-infection
#3
A S Vdovin, S Y Filkin, P R Yefimova, S A Sheetikov, N M Kapranov, Y O Davydova, E S Egorov, E G Khamaganova, M Y Drokov, L A Kuzmina, E N Parovichnikova, G A Efimov, V G Savchenko
Patients undergoing allogeneic hematopoietic stem cell transplantation have a high risk of cytomegalovirus reactivation, which in the absence of T-cell immunity can result in the development of an acute inflammatory reaction and damage of internal organs. Transfusion of the virus-specific donor T-lymphocytes represents an alternative to a highly toxic and often ineffective antiviral therapy. Potentially promising cell therapy approach comprises transfusion of cytotoxic T-lymphocytes, specific to the viral antigens, immediately after their isolation from the donor's blood circulation without any in vitro expansion...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914461/the-role-of-cytokines-in-the-development-of-atherosclerosis
#4
REVIEW
A R Fatkhullina, I O Peshkova, E K Koltsova
Atherosclerosis contributes to the development of many cardiovascular diseases, which remain the leading cause of death in developed countries. Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. It is caused by dyslipidemia and mediated by both innate and adaptive immune responses. Inflammation is a key factor at all stages of atherosclerosis progression. Cells involved in pathogenesis of atherosclerosis were shown to be activated by soluble factors, cytokines, that strongly influence the disease development...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914460/possible-mechanisms-of-acquisition-of-herpesvirus-virokines
#5
E A Gorshkova, E S Shilov
The genomes of certain types of human and primate herpesviruses contain functional homologs of important host cytokines (IL-6, IL-17, and IL-10), or so-called virokines. Virokines can interact with immune cell receptors, transmit a signal to them, and thus switch the type of immune response that facilitates viral infection development. In this work, we have summarized possible ways of virokine origin and proposed an evolutionary scenario of virokine acquisition with involvement of retroviral coinfection of the host...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914459/mechanisms-of-changes-in-immune-response-during-bacterial-coinfections-of-the-respiratory-tract
#6
REVIEW
E N Sviriaeva, K V Korneev, M S Drutskaya, D V Kuprash
Acute diseases of the respiratory tract are often caused by viral pathogens and accompanying secondary bacterial infections. It is known that the development of such bacterial complications is caused mainly by a decreased infiltration with immune system cells and by suppressed inflammation in the lungs. There are significant advances in understanding the mechanisms of secondary infections, although many details remain unclear. This review summarizes current knowledge of the molecular and cellular changes in the host organism that can influence the course of bacterial coinfections in the respiratory tract...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914458/inactivation-of-inflammasomes-by-pathogens-regulates-inflammation
#7
REVIEW
F Yu Garib, A P Rizopulu, A A Kuchmiy, V F Garib
Inflammatory response is initiated and sustained by the action of quintessential pro-inflammatory cytokines of immune system namely IL-1β and IL-18. The maturation process of those cytokines is ensured by caspase-1 enzymatic activity, that is in turn is tightly controlled by multiprotein complexes called inflammasomes. Inflammasomes are activated in cells of innate immune system in response to recognition of conservative parts of microbes (pathogen-associated molecular patterns) or by sensing molecular signs of tissue damage (damage-associated molecular patterns)...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914457/tumor-necrosis-factor-and-lymphotoxin-in-regulation-of-intestinal-inflammation
#8
REVIEW
E O Gubernatorova, A V Tumanov
Ulcerative colitis and Crohn's disease are the major forms of inflammatory bowel disease. Cytokines of the tumor necrosis factor (TNF) family play an important role in the regulation of intestinal inflammation. In this review, we discuss the function of key cytokines of this family - TNF and lymphotoxin (LT) - in mucosal healing, IgA production, and in control of innate lymphoid cells (ILCs), novel regulators of mucosal homeostasis in the gut. TNF plays a central role in the pathogenesis of inflammatory bowel diseases (IBD)...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914456/microbiota-induces-expression-of-tumor-necrosis-factor-in-postnatal-mouse-skin
#9
D V Yuzhakova, M V Shirmanova, A A Bocharov, I V Astrakhantseva, E A Vasilenko, E N Gorshkova, M S Drutskaya, E V Zagaynova, S A Nedospasov, A A Kruglov
Tumor necrosis factor (TNF) is a pleiotropic cytokine that regulates many important processes in the body. TNF production in a physiological state supports the structure of lymphoid organs and determines the development of lymphoid cells in hematopoiesis. However, chronic TNF overexpression leads to the development of various autoimmune disorders. Sites of TNF production in the naïve state remain unclear due to the lack of in vivo models. In the present study, we used TNF-2A-Kat reporter mice to monitor the expression of TNF in different tissues...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914455/mediators-and-biomarkers-of-inflammation-in-meningitis-cytokine-and-peptidome-profiling-of-cerebrospinal-fluid
#10
A A Belogurov, O M Ivanova, Y A Lomakin, R H Ziganshin, M I Vaskina, V D Knorre, E A Klimova, A G Gabibov, V T Ivanov, V M Govorun
Differential diagnosis of bacterial and viral meningitis is an urgent problem of the modern clinical medicine. Early and accurate detection of meningitis etiology largely determines the strategy of its treatment and significantly increases the likelihood of a favorable outcome for the patient. In the present work, we analyzed the peptidome and cytokine profiles of cerebrospinal fluid (CSF) of 17 patients with meningitis of bacterial and viral etiology and of 20 neurologically healthy controls. In addition to the identified peptides (potential biomarkers), we found significant differences in the cytokine status of the CSF of the patients...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914454/-suppressor-factor-of-neutrophils-a-short-story-of-a-long-term-misconception
#11
I A Linge, E V Kondratieva, T K Kondratieva, V A Makarov, V I Polshakov, O Yu Savelyev, A S Apt
A large body of evidence obtained during the last decade has demonstrated that neutrophils suppress T cell proliferation in different models of inflammation and cell interaction. The commonly used method for assessing cell proliferation and proliferation inhibition is measuring [3H]thymidine incorporation into cells. Earlier, we observed inhibition of [3H]thymidine uptake in experiments on neutrophil-mediated regulation of T cell response in tuberculosis immunity. Here, we used different types of proliferating cells to analyze the nature of the soluble "neutrophil factor" by a variety of methods (dialysis, HPLC, mass spectrometry, and NMR) and unambiguously demonstrated that neutrophils do not synthesize a specific factor inhibiting cell proliferation, but secrete high concentrations of extracellular thymidine that competitively inhibit [3H]thymidine incorporation...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914453/myeloid-derived-suppressor-cells-and-proinflammatory-cytokines-as-targets-for-cancer-therapy
#12
REVIEW
K-S N Atretkhany, M S Drutskaya
Myeloid-derived suppressor cells represent a heterogeneous population of immature myeloid cells. Under normal conditions, these cells differentiate into macrophages, dendritic cells, and granulocytes. However, in pathological states such as inflammation, infection, or tumor growth, there is an arrest of their differentiation that results in the accumulation of immature myeloid cells in the organism. In addition, these cells acquire a suppressor phenotype, expressing anti-inflammatory cytokines and reactive oxygen and nitrogen species, and suppress T-cell immune response...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914452/inflammatory-immune-infiltration-in-human-tumors-role-in-pathogenesis-and-prognostic-and-diagnostic-value
#13
REVIEW
A V Bogolyubova, P V Belousov
The cellular microenvironment directly and indirectly influences tumor development and possesses prognostic and in some cases diagnostic value. Over the years, understanding of structural organization of the immune/inflammatory moiety of neoplasms as well as in-depth phenotypic and transcriptomic profiling of its cellular components together provide more and more insights in both basic and translational medical science. In this review, we will discuss the specific roles of various stromal cells and their impact on neoplastic progression as well as address the use of quantitative and phenotypic analysis of immune/inflammatory infiltrate for diagnostics and predicting the clinical course of human malignancies...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914451/effects-of-fibroin-microcarriers-on-inflammation-and-regeneration-of-deep-skin-wounds-in-mice
#14
A Y Arkhipova, M A Nosenko, N V Malyuchenko, R V Zvartsev, A M Moisenovich, A S Zhdanova, T V Vasil'eva, E A Gorshkova, I I Agapov, M S Drutskaya, S A Nedospasov, M M Moisenovich
The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interesting area of regenerative medicine and biomedical research. In many ways, the regenerative potential of biological material is related to its ability to modulate the inflammatory response. At the same time, all foreign materials, once implanted into a living tissue, to varying degree cause an immune reaction...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914450/mechanisms-of-inflammatory-injury-of-renal-tubular-cells-in-a-cellular-model-of-pyelonephritis
#15
M A Morosanova, E Y Plotnikov, L D Zorova, I B Pevzner, V A Popkov, D N Silachev, S S Jankauskas, V A Babenko, D B Zorov
Previously, we have assembled a cellular model of pyelonephritis which contains a primary culture of renal tubular epithelial cells, mononuclear leukocytes, and bacterial lysate or lipopolysaccharide. After cocultivation of renal cells with leukocytes and bacterial lysate, proinflammatory changes were observed in the renal cells, followed by nitrosative and oxidative stress and cell death. The interaction of bacterial antigens not only with leukocytes, but also with epithelial cells of the renal tubules, was partially mediated by signaling pathways involving Toll-like receptors (TLR2 and TLR4)...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914449/molecular-and-cellular-mechanisms-of-inflammation
#16
D V Kuprash, S A Nedospasov
Inflammation is one of the most fundamental and pronounced protective reactions of the organism. From ancient times to the present day, complex and diverse patterns of inflammation development and their role in various diseases have attracted attention of investigators. This issue of Biokhimiya/Biochemistry (Moscow) includes experimental studies and reviews dedicated to various aspects of this important and interesting problem.
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27908248/do-mitochondria-have-an-immune-system
#17
V A Popkov, L D Zorova, I O Korvigo, D N Silachev, S S Jankauskas, V A Babenko, I B Pevzner, T I Danilina, S D Zorov, E Y Plotnikov, D B Zorov
The question if mitochondria have some kind of immune system is not trivial. The basis for raising this question is the fact that bacteria, which are progenitors of mitochondria, do have an immune system. The CRISPR system in bacteria based on the principle of RNA interference serves as an organized mechanism for destroying alien nucleic acids, primarily those of viral origin. We have shown that mitochondria are also a target for viral attacks, probably due to a related organization of genomes in these organelles and bacteria...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27908247/knockdown-of-minichromosome-maintenance-proteins-inhibits-foci-forming-of-mediator-of-dna-damage-checkpoint-1-in-response-to-dna-damage-in-human-esophageal-squamous-cell-carcinoma-te-1-cells
#18
Jinzhong Yu, Ruijie Wang, Jinfeng Wu, Zhongqin Dang, Qinsheng Zhang, Bo Li
Esophageal squamous cell carcinoma (ESCC) has a high morbidity in China and its treatment depends greatly on adjuvant chemotherapy. However, DNA damage repair in cancer cells severely affects the outcome of treatment. This study investigated the potential mechanism regarding mediator of DNA-damage checkpoint 1 (MDC1) and minichromosome maintenance proteins (MCMs) during DNA damage in ESCC. Recombinant vectors of MDC1 and MCMs with tags were constructed and transfected into human ESCC cell line TE-1. Immunoprecipitation and mass spectrometry were performed to screen the MCMs interacting with MDC1, and direct interaction was confirmed by glutathione S-transferase (GST) pull-down assay in vitro...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27908246/inhibition-of-chaperonin-groel-by-a-monomer-of-ovine-prion-protein-and-its-oligomeric-forms
#19
S S Kudryavtseva, Y Y Stroylova, I A Zanyatkin, T Haertle, V I Muronetz
The possibility of inhibition of chaperonin functional activity by amyloid proteins was studied. It was found that the ovine prion protein PrP as well as its oligomeric and fibrillar forms are capable of binding with the chaperonin GroEL. Besides, GroEL was shown to promote amyloid aggregation of the monomeric and oligomeric PrP as well as PrP fibrils. The monomeric PrP was shown to inhibit the GroEL-assisted reactivation of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The oligomers of PrP decelerate the GroEL-assisted reactivation of GAPDH, and PrP fibrils did not affect this process...
October 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27908245/perfect-hemihedral-twinning-in-crystals-of-the-%C3%AE-subunit-of-translation-initiation-factor-2-from-sulfolobus-solfataricus-cause-and-effect
#20
O V Kravchenko, O S Nikonov, N A Nevskaya, E A Stolboushkina, V I Arkhipova, M B Garber, S V Nikonov
The crystal structure of the γ-subunit of translation initiation factor 2 from the archaeon Sulfolobus solfataricus (SsoIF2γ) has been solved based on perfectly hemihedral twinned data. The protein was cocrystallized with the 10-fold molar excess of GTP analog (GDPCP) over protein. However, no nucleotide was found in the structure, and the model demonstrated the apo form of the protein. Two slightly different molecules in the asymmetric unit of the crystal are related by the non-crystallographic 2-fold axis and form a tightly associated dimer...
October 2016: Biochemistry. Biokhimii︠a︡
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