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Biochemistry. Biokhimii︠a︡

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https://www.readbyqxmd.com/read/29223166/protein-poly-adp-ribosyl-ation-system-changes-in-development-and-aging-as-well-as-due-to-restriction-of-cell-proliferation
#1
REVIEW
G A Shilovsky, S I Shram, G V Morgunova, A N Khokhlov
It is well known that the number of dividing cells in an organism decreases with age. The average rate of cell division in tissues and organs of a mature organism sharply decreases, which is probably a trigger for accumulation of damage leading to disturbance of genome integrity. This can be a cause for the development of many age-related diseases and appearance of phenotypic and physiological signs of aging. In this connection, the protein poly(ADP-ribosyl)ation system, which is activated in response to appearance of various DNA damage, attracts great interest...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223165/role-of-microrna-in-development-of-instability-of-atherosclerotic-plaques
#2
REVIEW
I A Koroleva, M S Nazarenko, A N Kucher
MicroRNAs are small noncoding single-stranded RNAs that regulate gene expression. Today, we see an increasing number of studies highlighting the important role of microRNAs in the development and progression of cardiovascular diseases caused by atherosclerotic lesions of arteries. We review the available scientific data on association of the expression of these biomolecules with instability of atherosclerotic plaques in animal models and humans. We made special emphasis on miR-21, -100, -127, -133, -143/145, -221/222, and -494 because they were analyzed in more than one study...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223164/the-mitochondrial-genome-of-the-moss-brachythecium-rivulare-hypnales-brachytheciaceae
#3
D V Goryunov, M D Logacheva, M S Ignatov, I A Milyutina, A V Fedorova, A V Troitsky
The mitochondrial genome of the pleurocarpous moss Brachythecium rivulare has been sequenced and annotated. The genome consists of 104,460 base pairs and has approximately the same gene set and organization as other bryophyte mitogenomes. Whole mitochondrial genome comparison between B. rivulare and Physcomitrella patens, Tetraphis pellucida, Anomodon rugelii, and Anomodon attenuatus was performed. The primary cause of bryophyte mitochondrial gene length variation was found to be numerous indels in the introns...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223163/neuroprotective-properties-of-endocannabinoids-n-arachidonoyl-dopamine-and-n-docosahexaenoyl-dopamine-examined-in-neuronal-precursors-derived-from-human-pluripotent-stem-cells
#4
E V Novosadova, E L Arsenyeva, E S Manuilova, L G Khaspekov, M Yu Bobrov, V V Bezuglov, S N Illarioshkin, I A Grivennikov
Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine (NADA) and N-docosahexaenoyl dopamine (DHDA) were examined in neuronal precursor cells differentiated from human induced pluripotent stem cells and subjected to oxidative stress. Both compounds exerted neuroprotective activity, which was enhanced by elevating the concentration of the endocannabinoids within the 0.1-10 µM range. However, both agents at 10 µM concentration showed a marked toxic effect resulting in death of ~30% of the cells...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223162/spectral-kinetic-analysis-of-recombination-reaction-of-heme-centers-of-bd-type-quinol-oxidase-from-escherichia-coli-with-carbon-monoxide
#5
S A Siletsky, A V Dyuba, D A Elkina, M V Monakhova, V B Borisov
Recombination of the isolated, fully reduced bd-type quinol oxidase from Escherichia coli with carbon monoxide was studied by pulsed absorption spectrophotometry with microsecond time resolution. Analysis of the kinetic phases of recombination was carried out using the global analysis of multiwavelength kinetic data ("Global fitting"). It was found that the unresolved photodissociation of CO is followed by a stepwise (with four phases) recombination with characteristic times (τ) of about 20 µs, 250 µs, 1...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223161/cell-free-expression-purification-and-characterization-of-the-functional-%C3%AE-2-adrenergic-receptor-for-multianalyte-detection-of-%C3%AE-agonists
#6
Jian Wang, Yuan Liu, Junhua Zhang, Zhengzheng Han, Wei Wang, Yang Liu, Dong Wei, Wei Huang
Large-scale expression of β2-adrenergic receptor (β2-AR) in functional form is necessary for establishment of receptor assays for detecting illegally abused β-adrenergic agonists (β-agonists). Cell-based heterologous expression systems have manycritical difficulties in synthesizing this membrane protein, such as low protein yields and aberrant folding. To overcome these challenges, the main objective of the present work was to synthesize large amounts of functional β2-AR in a cell-free system based on Escherichia coli extracts...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223160/cathelicidin-ll37-promotes-epithelial-and-smooth-muscle-like-differentiation-of-adipose-derived-stem-cells-through-the-wnt-%C3%AE-catenin-and-nf-%C3%AE%C2%BAb-pathways
#7
Yongwei Li, Zhengfei Shan, Bin Yang, Diandong Yang, Changping Men, Yuanshan Cui, Jitao Wu
Ureter reconstruction is a difficult procedure in urology. Adipose-derived stem cells (ADSCs), along with multipotency and self-renewal capacity, are a preferred choice for tissue engineering-based ureteral reconstruction. We explored the synergic role of cathelicidin LL37 (LL37) in epithelial and smooth-muscle-like differentiation. ADSCs were separated from adipose tissues of mouse and characterized by flow cytometry. The ADSCs were then stably transfected with pGC-FU-GFP (pGC) or pGC containing full-length LL37 (pGC-LL37), respectively...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223159/factors-beyond-enolase-2-and-mitochondrial-lysyl-trna-synthetase-precursor-are-required-for-trna-import-into-yeast-mitochondria
#8
M V Baleva, M Meyer, N Entelis, I Tarassov, P Kamenski, B Masquida
In yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to re-direct it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223158/salusin-%C3%AE-attenuates-inflammatory-responses-in-vascular-endothelial-cells
#9
Maryam Esfahani, Masoud Saidijam, Mohammad Taghi Goodarzi, Ahmad Movahedian, Rezvan Najafi
Atherosclerosis accounts for numerous cardiovascular diseases, and cytokines have a critical role in acceleration or suppression of disease. Salusin-α presents a new class of bioactive peptides that can have anti-atherogenic properties. Therefore, the effects of salusin-α on the expression of some pro- and anti-inflammatory cytokines and on TNF-α-induced inflammatory responses in human umbilical vein endothelial cells (HUVECs) were examined. The involvement of the NF-κB pathway in effects of salusin-α in HUVECs was checked using Bay 11-7082 as an NF-κB inhibitor...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223157/self-organization-of-recombinant-membrane-porin-ompf-from-yersinia-pseudotuberculosis-in-aqueous-environments
#10
E V Sidorin, V A Khomenko, N Yu Kim, P S Dmitrenok, A M Stenkova, O D Novikova, T F Solov'eva
Recombinant porin OmpF (an integral protein of bacterial outer membrane) from Yersinia pseudotuberculosis was synthesized in Escherichia coli cells as inclusion bodies. By combining the methods of anion-exchange and gel filtration chromatographies, recombinant OmpF (rOmpF) was isolated as an individual protein in its denatured state, and its characteristic properties (molecular mass, N-terminal amino acid sequence, and hydrodynamic radius of the protein in 8 M urea solution) were determined. According to the data of gel filtration, dynamic light scattering, optical spectroscopy, and binding of the hydrophobic fluorescent probe 8-anilino-1-naphthalenesulfonic acid, the rOmpF is fully unfolded in 8 M urea and exists in random coil conformation...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223156/biochemical-properties-and-phylogeny-of-hydroxypyruvate-reductases-from-methanotrophic-bacteria-with-different-c1-assimilation-pathways
#11
S Y But, S V Egorova, V N Khmelenina, Y A Trotsenko
In the aerobic methanotrophic bacteria Methylomicrobium alcaliphilum 20Z, Methylococcus capsulatus Bath, and Methylosinus trichosporium OB3b, the biochemical properties of hydroxypyruvate reductase (Hpr), an indicator enzyme of the serine pathway for assimilation of reduced C1-compounds, were comparatively analyzed. The recombinant Hpr obtained by cloning and heterologous expression of the hpr gene in Escherichia coli catalyzed NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but did not catalyze the reverse reactions of D-glycerate or glycolate oxidation...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223155/recombinant-human-erythropoietin-with-additional-processable-protein-domains-purification-of-protein-synthesized-in-escherichia-coli-heterologous-expression-system
#12
T M Grunina, A V Demidenko, A M Lyaschuk, M S Poponova, Z M Galushkina, L A Soboleva, S A Cherepushkin, N B Polyakov, D A Grumov, A I Solovyev, V G Zhukhovitsky, I S Boksha, M E Subbotina, A V Gromov, V G Lunin, A S Karyagina
Three variants of human recombinant erythropoietin (rhEPO) with additional N-terminal protein domains were obtained by synthesis in an Escherichia coli heterologous expression system. These domains included (i) maltose-binding protein (MBP), (ii) MBP with six histidine residues (6His) in N-terminal position, (iii) s-tag (15-a.a. oligopeptide derived from bovine pancreatic ribonuclease A) with N-terminal 6His. Both variants of the chimeric protein containing MBP domain were prone to aggregation under nondenaturing conditions, and further purification of EPO after the domain cleavage by enterokinase proved to be impossible...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223154/antiinflammatory-effect-of-rosiglitazone-via-modulation-of-mrna-stability-of-interleukin-10-and-cyclooxygenase-2-in-astrocytes
#13
E V Pankevich, A A Astakhova, D V Chistyakov, M G Sergeeva
Investigation of molecular mechanisms of proinflammatory stimuli signaling in astrocytes is important for understanding their role in pathogenesis of central nervous system diseases as well as in functioning of the innate immunity system in non-immune cells. Here we show that lipopolysaccharide (LPS) stimulation of primary rat astrocytes led to conventional inflammatory response: increase in both proinflammatory (tumor necrosis factor, TNFα; prostaglandin E2, PGE2) and antiinflammatory marker (interleukin 10, IL-10) levels...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223153/temperature-dependence-of-tryptophan-fluorescence-lifetime-in-aqueous-glycerol-and-trehalose-solutions
#14
V V Gorokhov, P P Knox, B N Korvatovskiy, N Kh Seifullina, S N Goryachev, V Z Paschenko
The temperature dependences of tryptophan fluorescence decay kinetics in aqueous glycerol and 1 M trehalose solutions were examined. The fluorescence decay kinetics were recorded in the spectral region of 292.5-417.5 nm with nanosecond time resolution. The kinetics curves were approximated by the sum of three exponential terms, and the spectral distribution (DAS) of these components was determined. An antisymbatic course of fluorescence decay times of two (fast and medium) components in the temperature range from -60 to +10°C was observed...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223152/electron-transfer-through-the-acceptor-side-of-photosystem-i-interaction-with-exogenous-acceptors-and-molecular-oxygen
#15
REVIEW
D A Cherepanov, G E Milanovsky, A A Petrova, A N Tikhonov, A Yu Semenov
This review considers the state-of-the-art on mechanisms and alternative pathways of electron transfer in photosynthetic electron transport chains of chloroplasts and cyanobacteria. The mechanisms of electron transport control between photosystems (PS) I and II and the Calvin-Benson cycle are considered. The redistribution of electron fluxes between the noncyclic, cyclic, and pseudocyclic pathways plays an important role in the regulation of photosynthesis. Mathematical modeling of light-induced electron transport processes is considered...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223151/the-power-and-limitations-of-influenza-virus-hemagglutinin-assays
#16
REVIEW
N B Ustinov, E G Zavyalova, I G Smirnova, A M Kopylov
Influenza virus hemagglutinins (HAs) are surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus internalization. Development of methods for the inhibition of these processes drives progress in the design of new antiviral drugs. The state of the isolated HA (i.e. combining tertiary structure and extent of oligomerization) is defined by multiple factors, like the HA source and purification method, posttranslational modifications, pH, etc. The HA state affects HA functional activity and significantly impacts the results of numerous HA assays...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29223150/modern-approaches-for-identification-of-modified-nucleotides-in-rna
#17
REVIEW
J A Filippova, D V Semenov, E S Juravlev, A B Komissarov, V A Richter, G A Stepanov
This review considers approaches for detection of modified monomers in the RNA structure of living organisms. Recently, some data on dynamic alterations in the pool of modifications of the key RNA species that depend on external factors affecting the cells and physiological conditions of the whole organism have been accumulated. The recent studies have presented experimental data on relationship between the mechanisms of formation of modified/minor nucleotides of RNA in mammalian cells and the development of various pathologies...
November 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29037143/erratum-to-how-fucose-of-blood-group-glycotypes-programs-human-gut-microbiota-biochemistry-moscow-82-973-2017
#18
S V Kononova
This corrects the article DOI: 10.1134/S0006297917090012.
October 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29037142/enzyme-substrate-reporters-for-evaluation-of-substrate-specificity-of-hif-prolyl-hydroxylase-isoforms
#19
A I Osipyants, N A Smirnova, A Yu Khristichenko, D M Hushpulian, S V Nikulin, T A Chubar, A A Zakhariants, V I Tishkov, I G Gazaryan, A A Poloznikov
An organism naturally responds to hypoxia via stabilization of hypoxia-inducible factor (HIF). There are three isoforms of HIFα subunits whose stability is regulated by three isozymes of HIF prolyl hydroxylase (PHD1-3). Despite intense studies on recombinant enzyme isoforms using homogeneous activity assay, there is no consensus on the PHD isoform preference for the HIF isoform as a substrate. This work provides a new approach to the problem of substrate specificity using cell-based reporters expressing the enzyme and luciferase-labeled substrate pair encoded in the same expression vector...
October 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29037141/analysis-of-four-circulating-complexes-of-insulin-like-growth-factor-binding-proteins-in-human-blood-during-aging
#20
O Nedić, M Šunderić, N Gligorijević, V Malenković, G Miljuš
The primary role of insulin-like growth factor binding proteins (IGFBPs) is to regulate availability of IGFs for interacting with receptors, but IGFBPs perform IGF-independent actions as well. The availability and activity of IGFBPs in the circulation is influenced primarily by their concentration and structural modifications, but possibly also by interaction with major plasma proteins such as transferrin, alpha-2-macroglobulin (α2M), and fibrinogen. Four types of circulating IGFBP complexes were examined in this study by immuno- and ligand-binding assays in adults of different age...
October 2017: Biochemistry. Biokhimii︠a︡
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