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Biochemistry. Biokhimii︠a︡

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https://www.readbyqxmd.com/read/28371610/short-exogenous-peptides-regulate-expression-of-cle-knox1-and-grf-family-genes-in-nicotiana-tabacum
#1
L I Fedoreyeva, T A Dilovarova, V V Ashapkin, Yu Ts Martirosyan, V Kh Khavinson, P N Kharchenko, B F Vanyushin
Exogenous short biologically active peptides epitalon (Ala-Glu-Asp-Gly), bronchogen (Ala-Glu-Asp-Leu), and vilon (Lys-Glu) at concentrations 10(-7)-10(-9) M significantly influence growth, development, and differentiation of tobacco (Nicotiana tabacum) callus cultures. Epitalon and bronchogen, in particular, both increase growth of calluses and stimulate formation and growth of leaves in plant regenerants. Because the regulatory activity of the short peptides appears at low peptide concentrations, their action to some extent is like that of the activity of phytohormones, and it seems to have signaling character and epigenetic nature...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371609/trastuzumab-and-pertuzumab-plant-biosimilars-modification-of-asn297-linked-glycan-of-the-mabs-produced-in-a-plant-with-fucosyltransferase-and-xylosyltransferase-gene-knockouts
#2
T V Komarova, E V Sheshukova, E N Kosobokova, M V Serebryakova, V S Kosorukov, V N Tashlitsky, Y L Dorokhov
Plant biosimilars of anticancer therapeutic antibodies are of interest not only because of the prospects of their practical use, but also as an instrument and object for study of plant protein glycosylation. In this work, we first designed a pertuzumab plant biosimilar (PPB) and investigated the composition of its Asn297-linked glycan in comparison with trastuzumab plant biosimilar (TPB). Both biosimilars were produced in wild-type (WT) Nicotiana benthamiana plant (PPB-WT and TPB-WT) and transgenic ΔXTFT N...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371608/studying-factors-involved-in-biogenesis-of-lysobacter-sp-xl1-outer-membrane-vesicles
#3
I V Kudryakova, N E Suzina, N G Vinokurova, N A Shishkova, N V Vasilyeva
The Gram-negative bacterium Lysobacter sp. XL1 produces outer membrane vesicles that are heterogeneous in size, density, and protein composition. One of the subpopulations is secretory vesicles for lytic protease L5 of Lysobacter sp. XL1 (Kudryakova et al. (2015) FEMS Microbiol. Lett., 362, fnv137). Protein L5 was assumed to influence biogenesis of these secretory vesicles that contain it. Using a Pseudomonas fluorescens Q2-87/B expression system, it was shown that the recombinant L5 protein may act as a factor of vesicle biogenesis...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371607/femtosecond-and-picosecond-dynamics-of-recombinant-bacteriorhodopsin-primary-reactions-compared-to-the-native-protein-in-trimeric-and-monomeric-forms
#4
O A Smitienko, O V Nekrasova, A V Kudriavtsev, M A Yakovleva, I V Shelaev, F E Gostev, D A Dolgikh, I B Kolchugina, V A Nadtochenko, M P Kirpichnikov, T B Feldman, M A Ostrovsky
Photochemical reaction dynamics of the primary events in recombinant bacteriorhodopsin (bRrec) was studied by femtosecond laser absorption spectroscopy with 25-fs time resolution. bRrec was produced in an Escherichia coli expression system. Since bRrec was prepared in a DMPC-CHAPS micelle system in the monomeric form, its comparison with trimeric and monomeric forms of the native bacteriorhodopsin (bRtrim and bRmon, respectively) was carried out. We found that bRrec intermediate I (excited state of bR) was formed in the range of 100 fs, as in the case of bRtrim and bRmon...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371606/structure-and-gene-cluster-of-the-k93-capsular-polysaccharide-of-acinetobacter-baumannii-b11911-containing-5-n-acetyl-7-n-r-3-hydroxybutanoyl-pseudaminic-acid
#5
A A Kasimova, M M Shneider, N P Arbatsky, A V Popova, A S Shashkov, K A Miroshnikov, Veeraraghavan Balaji, Indranil Biswas, Yu A Knirel
Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with one- and two-dimensional (1)H and (13)C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-d-Galp-(1→6)-β-d-Galp-(1→3)-d-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371605/microrna-294-promotes-cellular-proliferation-and-motility-through-the-pi3k-akt-and-jak-stat-pathways-by-upregulation-of-nras-in-bladder-cancer
#6
Yongwei Li, Zhengfei Shan, Chu Liu, Diandong Yang, Jitao Wu, Changping Men, Yankai Xu
In our study we examined the role of microRNA-294 (miR-294) in bladder cancer and related mechanisms. Real-time polymerase chain reaction (RT-PCR) was performed to determine the expression level of miR-294. Western blot was used to determine the expression of NRAS, mainly factors in the PI3K/AKT and JAK/STAT pathways. Cell counting kit-8 assay, clonogenic assay, wound-healing assay, transwell and flow cytometry were used to explore, respectively, cell proliferation, survival, migration, invasion, and apoptosis of bladder cancer cell line T24...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371604/polyols-have-unique-ability-to-refold-protein-as-compared-to-other-osmolyte-types
#7
G S Sharma, L R Singh
Effects of solvent environments on protein refolding have gained significant attention due to their biotechnological and pharmaceutical applications. Recent advances have shown that a number of organic osmolytes have the unique ability to induce proper folding of several misfolded proteins and simultaneously inhibit aggregation during the process. In the present study, we investigated the effects of polyol osmolytes on the refolding of guanidinium chloride-denatured ribonuclease-A (RNase-A) and compared it with that of other osmolyte types...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371603/triosephosphates-as-intermediates-of-the-crabtree-effect
#8
S S Sokolov, O V Markova, K D Nikolaeva, I A Fedorov, F F Severin
An increase in glucose concentration in the medium rapidly decreases respiration rate in many cell types, including tumor cells. The molecular mechanism of this phenomenon, the Crabtree effect, is still unclear. It was shown earlier that adding the intermediate product of glycolysis fructose-1,6-bisphosphate to isolated mitochondria suppresses their respiration. To study possible roles of glycolytic intermediates in the Crabtree effect, we used a model organism, the yeast Saccharomyces cerevisiae. To have the option to rapidly increase intracellular concentrations of certain glycolytic intermediates, we used mutant cells with glycolysis blocked at different stages...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371602/toward-gene-therapy-of-hypertension-experimental-study-on-hypertensive-isiah-rats
#9
M N Repkova, A S Levina, A A Seryapina, N V Shikina, E V Bessudnova, V F Zarytova, A L Markel
TiO2-based nanocomposites were prepared to deliver oligonucleotides into cells. The nanocomposites were designed by the immobilization of polylysine-containing oligonucleotides on TiO2-nanoparticles (TiO2·PL-DNA). We showed for the first time the possibility of using the proposed nanocomposites for treatment of hypertensive disease by introducing them into hypertensive ISIAH rats developed as a model of stress-sensitive arterial hypertension. The mRNA of the gene encoding angiotensin I-converting enzyme (ACE1) involved in the synthesis of angiotensin II was chosen as a target...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371601/attenuation-of-focal-adhesion-kinase-reduces-lipopolysaccharide-induced-inflammation-injury-through-inactivation-of-the-wnt-and-nf-%C3%AE%C2%BAb-pathways-in-a549-cells
#10
D Bai, S Cong, L P Zhu
Overall analysis and understanding of mechanisms are of great importance for treatment of infantile pneumonia due to its high morbidity and mortality worldwide. In this study, we preliminarily explored the function and mechanism of focal adhesion kinase (FAK) in regulation of inflammatory response induced by lipopolysaccharides in A549 cells. Flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, quantitative reverse transcription polymerase chain reaction, and Western blot analysis were used to explore the correlation of FAK expression with cell apoptosis, viability, and the inflammatory cytokine activity in A549 cells...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371600/hur-stabilizes-lnc-sox5-mrna-to-promote-tongue-carcinogenesis
#11
Lifang Wang, Shucheng Ye, Junye Wang, Zhenfang Gu, Yanhui Zhang, Chunmei Zhang, Xuezhen Ma
Long noncoding RNAs (lncRNAs) have been recently regarded as systemic regulators in multiple biological processes including tumorigenesis. In this study, we report an ultra-highly expressed lncRNA, lnc-Sox5, in tongue tumor tissues. The results imply that lnc-Sox5 may play vital role in tongue carcinoma progression. We observed that the growth of Tca8113 cells was suppressed by lnc-Sox5 downregulation. Additionally, lnc-Sox5 knockdown simultaneously increased Tca8113 cell apoptosis, but the cell cycle was arrested...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371599/formyl-peptide-receptor-polymorphisms-27-most-possible-ways-for-phagocyte-dysfunction
#12
REVIEW
S S Skvortsov, A G Gabdoulkhakova
Formyl peptide receptors (FPRs) expressed by mammalian myeloid cells are the important part of innate immunity. They belong to the seven-transmembrane domain class of receptors coupled to heterotrimeric GTP-binding proteins. Binding of the receptor with a wide spectrum of exogenous and endogenous ligands triggers such defensive phagocyte reactions as chemotaxis, secretory degranulation, and respiratory burst, keeping a balance of inflammatory and antiinflammatory processes in the organism. The association between single nucleotide polymorphisms in the gene of FPR1 receptor resulting in disruption of the receptor structure and the development of certain pathologies accompanied with inflammation, such as aggressive periodontitis, macular degeneration, and even gastric cancer (Maney, P...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371598/natural-and-chemotherapy-induced-clonal-evolution-of-tumors
#13
REVIEW
M K Ibragimova, M M Tsyganov, N V Litviakov
Evolution and natural selection of tumoral clones in the process of transformation and the following carcinogenesis can be called natural clonal evolution. Its main driving factors are internal: genetic instability initiated by driver mutations and microenvironment, which enables selective pressure while forming the environment for cell transformation and their survival. We present our overview of contemporary research dealing with mechanisms of carcinogenesis in different localizations from precancerous pathologies to metastasis and relapse...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28371597/role-of-proton-pumps-in-tumorigenesis
#14
REVIEW
V A Kobliakov
One of the differences between normal and cancer cells is lower pH of the extracellular space in tumors. Low pH in the extracellular space activates proteases and stimulates tumor invasion and metastasis. Tumor cells display higher level of the HIF1α transcription factor that promotes cell switch from mitochondrial respiration to glycolysis. The terminal product of glycolysis is lactate. Lactate formation from pyruvate is catalyzed by the specific HIF1α-dependent isoform of lactate dehydrogenase A. Because lactate accumulation is deleterious for the cell, it is actively exported by monocarboxylate transporters...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320281/cerebral-mechanisms-of-hypoxic-ischemic-postconditioning
#15
REVIEW
O V Vetrovoy, E A Rybnikova, M O Samoilov
This review analyzes recent data on mechanisms of cerebral hypoxia and the protective methods of hypoxic and ischemic postconditioning, as well as their interrelationship with the key mechanisms responsible for neuroprotection and neuroplasticity. Upregulation of expression of antiapoptotic factors and neurotrophins and modulation of activity of several protein kinases and transcription factors such as hypoxia-inducible factor-1 (HIF-1) are considered as the most important aspects in the neuroprotective potential of postconditioning...
March 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320280/molecular-mechanisms-mediating-involvement-of-glial-cells-in-brain-plastic-remodeling-in-epilepsy
#16
REVIEW
L G Khaspekov, L E Frumkina
In this review we summarize published data on the involvement of glial cells in molecular mechanisms underlying brain plastic reorganization in epilepsy. The role of astrocytes as glial elements in pathological plasticity in epilepsy is discussed. Data on the involvement of aquaporin-4 in epileptogenic plastic changes and on participation of microglia and extracellular matrix in dysregulation of synaptic transmission and plastic remodeling in epileptic brain tissue are reviewed.
March 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320279/plasticity-of-central-and-peripheral-sources-of-noradrenaline-in-rats-during-ontogenesis
#17
N S Bondarenko, L K Dilmukhametova, A Yu Kurina, A R Murtazina, A Ya Sapronova, A P Sysoeva, M V Ugrumov
The morphogenesis of individual organs and the whole organism occurs under the control of intercellular chemical signals mainly during the perinatal period of ontogenesis in rodents. In this study, we tested our hypothesis that the biologically active concentration of noradrenaline (NA) in blood in perinatal ontogenesis of rats is maintained due to humoral interaction between its central and peripheral sources based on their plasticity. As one of the mechanisms of plasticity, we examined changes in the secretory activity (spontaneous and stimulated release of NA) of NA-producing organs under deficiency of its synthesis in the brain...
March 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320278/cooperative-synthesis-of-dopamine-in-rat-mediobasal-hypothalamus-as-a-compensatory-mechanism-in-hyperprolactinemia
#18
A U Kurina, T S Pronina, L K Dilmukhametova, G V Maleev, M V Ugrumov
Dopamine (DA), synthesized in the mediobasal hypothalamus by dopaminergic neurons containing two enzymes of DA synthesis - tyrosine hydroxylase and decarboxylase of aromatic L-amino acids, or by monoenzymatic non-dopaminergic neurons containing one DA synthesis enzyme in cooperation, is known to have an inhibitory effect on prolactin secretion. Deterioration of this inhibitory control leads to an increase in prolactin concentration in the blood and to the development of hyperprolactinemia syndrome. In a rat model of hyperprolactinemia induced by administration of a neurotoxin causing degeneration of dopaminergic and noradrenergic neurons, the level of DA first decreases, leading to an increase in prolactin level (decompensation stage), while later both levels are restored to normal (compensation stage)...
March 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320277/mechanisms-of-brain-glucocorticoid-resistance-in-stress-induced-psychopathologies
#19
REVIEW
V M Merkulov, T I Merkulova, N P Bondar
Exposure to stress activates the hypothalamic-pituitary-adrenal axis and leads to increased levels of glucocorticoid (GC) hormones. Prolonged elevation of GC levels causes neuronal dysfunction, decreases the density of synapses, and impairs neuronal plasticity. Decreased sensitivity to glucocorticoids (glucocorticoid resistance) that develops as a result of chronic stress is one of the characteristic features of stress-induced psychopathologies. In this article, we reviewed the published data on proposed molecular mechanisms that contribute to the development of glucocorticoid resistance in brain, including changes in the expression of the glucocorticoid receptor (GR) gene, biosynthesis of GR isoforms, and GR posttranslational modifications...
March 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28320276/effects-of-short-term-exposure-to-lithium-on-antiapoptotic-bcl-xl-protein-expression-in-cortex-and-hippocampus-of-rats-after-acute-stress
#20
N N Dygalo, A V Bannova, E V Sukhareva, G T Shishkina, K A Ayriyants, T S Kalinina
The antiapoptotic protein Bcl-xL is involved in development of neurobiological resilience to stress; hence, the possibility of use of psychotropic drugs to increase its expression in brain in response to stress is of considerable interest. Lithium is a neurotropic drug widely used in psychiatry. In work, we studied effects of lithium administration (for 2 or 7 days) on the expression of Bcl-xL mRNA and protein in the hippocampi and cortices of rats subjected to stress that induced depression-like behavior in the animals...
March 2017: Biochemistry. Biokhimii︠a︡
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