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Journal of Pharmacy and Pharmacology

Pengli Li, Jiejie Hao, Haihua Li, Huashi Guan, Chunxia Li
OBJECTIVES: Propylene glycol alginate sodium sulfate (PSS) is poorly absorbed by oral administration due to its large molecular weight and slightly degradability in stomach acidic environment. Here, a novel enteric-coated nano formulation of PSS (enteric PSS-NP) was prepared to improve its bioavailability and efficacy. METHODS: The enteric PSS-NP was prepared by double (W1 /O/W2 ) emulsion and solvent evaporation method. The drug release characteristics in vitro were studied in artificial gastrointestinal fluid...
March 12, 2018: Journal of Pharmacy and Pharmacology
Suelen S da Silva, Sandra S Mizokami, Jacqueline R Fanti, Idessania N Costa, Juliano Bordignon, Ionice Felipe, Wander R Pavanelli, Waldiceu A Verri, Ivete Conchon Costa
OBJECTIVES: To evaluate the analgesic effect of Glucantime (antimoniate N-methylglucamine) in Leishmania amazonensis infection and complete Freund's adjuvant (CFA), chronic paw inflammation model, in BALB/c mice. METHODS: Two models of chronic inflammatory pain in BALB/c mice paw were used: infection with L. amazonensis and CFA stimulation. Both animals models received daily treatment with Glucantime (10 mg/kg, i.p.) and during the treatment was measured the mechanical hyperalgesia with electronic version of von Frey filaments...
March 12, 2018: Journal of Pharmacy and Pharmacology
Mai Hashimoto, Kazuaki Taguchi, Takako Ishiguro, Satoru Kohgo, Shuhei Imoto, Keishi Yamasaki, Hiroaki Mitsuya, Masaki Otagiri
OBJECTIVES: 4'-cyano-2'-deoxyguanosine (CdG), a novel nucleoside analogue, has a high degree of antiviral activity against the chronic hepatitis B virus (HBV). The objective of this study was to develop an analytical method for quantitatively determining CdG levels in biological samples by liquid chromatography-mass spectrometry (LC/MS) and to investigate the pharmacokinetic properties of CdG in rats after intravenous and oral administration. METHODS: An analytical method using a UPLC system interfaced with a TOF-MS system was developed and validated...
March 12, 2018: Journal of Pharmacy and Pharmacology
Mahmoud A El-Saied, Mansour Sobeh, Walied Abdo, Osama M Badr, Lamiaa T Youssif, Ibrahim H Elsayed, Samir M Osman, Michael Wink
OBJECTIVES: The aim of this study was to investigate the potential anticancer properties of a methanol extract of Rheum palmatum roots against diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) in rats and to characterize its phytoconstituents. METHODS: HPLC-PDA-MS/MS was used to profile the secondary metabolites in R. palmatum root extract. HCC was induced using diethylnitrosamine (DENA). The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT), alpha-fetoprotein (AFP), total proteins, serum albumin and serum globulin was determined...
March 9, 2018: Journal of Pharmacy and Pharmacology
Artur Martynov, Gennady Didenko, Boris Farber, Sophya Farber, Olena Cruts
OBJECTIVES: Many adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and also provides the absence toxicity in the fRNA. METHODS: For the immunotherapy, we used immunotropic 70 kDa lectin Bacillus subtilis B-7025...
March 9, 2018: Journal of Pharmacy and Pharmacology
Shipeng Chen, Luo Yang, Jie Feng
OBJECTIVES: To explore the apoptotic effects and underlying mechanisms of nitidine chloride (NC) in epithelial ovarian cancer. METHODS: The MTT cell proliferation assay was used to detect the inhibitory effects of different concentrations of NC (0, 0.3125, 0.625, 1.25, 2.5, 5 and 10 μg/ml) in SKOV3 ovarian carcinoma cells. The number of apoptotic cells was observed by Hoechst staining and measured by flow cytometry. Quantitative PCR was used to measure the expression of Fas, Fas-associated death domain-containing protein (FADD), caspase-8 and caspase-3...
March 7, 2018: Journal of Pharmacy and Pharmacology
Nathalia Regina F Leal, Mariana Vieira Vigliano, Fabiana A Pinto, Thayane V de Sousa, Leosvaldo S M Velozo, Kátia C C Sabino, Maria da Graça Justo, Marsen Garcia Pinto Coelho
OBJECTIVES: To evaluate the anti-inflammatory potential of Pterodon polygalaeflorus hexane extract (HE) and its fractions on macrophage migration in vitro and in vivo. METHODS: Hexane extract from P. polygalaeflorus fruits was fractionated and yielded four fractions. RAW 264.7 cells were treated with samples to evaluate cell viability (MTT assay), cell migration (wound healing and transwell assays), CD14 expression (flow cytometry), iNOS and cytokine mRNA expression (RT-qPCR), NO (Griess reaction) and cytokine (ELISA) production...
March 7, 2018: Journal of Pharmacy and Pharmacology
Xingzhen Zhang, Lei Peng, Anchang Liu, Jianbo Ji, Lixia Zhao, Guangxi Zhai
OBJECTIVES: To evaluate the effects of tetrahydrocurcumin (THC) on the radiosensitivity of glioma cells and the possible molecular mechanism. METHODS: MTT assay, colony forming and wound healing assays were performed to detect the proliferation, radiosensitivity and migration of cells with various treatments. Cell apoptosis, cell cycle and GHS level were determined for exploring potent sensitization mechanism of THC. Meanwhile, protein expressions of cyclin D1 and PCNA were also measured...
March 1, 2018: Journal of Pharmacy and Pharmacology
Pablo Torres-Vergara, Jeffrey Penny
OBJECTIVES: The effects of anti-inflammatory glucocorticoids dexamethasone (DX) and hydrocortisone (HC), pro-inflammatory cytokine interleukin-1β (IL-1β) and dietary long-chain polyunsaturated fatty acids (PUFAs) on expression and activity of the ATP-binding cassette transporter P-glycoprotein (P-GP) were studied in porcine brain endothelial cells (PBECs). METHODS: Primary PBECs were treated for 24 h with glucocorticoids, IL-1β and long-chain PUFAs. P-GP activity was determined by measuring intracellular calcein accumulation and P-GP expression by Western blotting...
March 1, 2018: Journal of Pharmacy and Pharmacology
Dayane P de Araújo, Patrícia C N Nogueira, Alan Diego C Santos, Roberta de Oliveira Costa, Jalles D de Lucena, Carlos Vinicius Jataí Gadelha-Filho, Francisco Arnaldo V Lima, Kelly Rose T Neves, Luzia Kalyne Almeida M Leal, Edilberto R Silveira, Glauce S B Viana
OBJECTIVES: Aspidosperma species are used for several diseases, especially for malaria in Brazil. Although the genus is object of pharmacological studies, almost none are found on Aspidosperma pyrifolium. We investigate neuroprotective, antioxidant and anti-inflammatory properties of the APSE-Aq fraction (benzoic acid glycosylated derivative) on Parkinson's disease model. METHODS: Male Wistar rats were subjected to a 6-hydroxydopamine injection into the right striatum and treated or not with APSE-Aq (100 or 200 mg/kg, p...
February 28, 2018: Journal of Pharmacy and Pharmacology
Lichun Zhao, Dandan Sun, Haibin Lu, Bing Han, Guangyuan Zhang, Qingxiang Guan
OBJECTIVES: A system of stearic acid (SA)-modified Bletilla striata polysaccharide (BSP) micelles was developed for the targeted delivery of docetaxel (DTX) as a model anticancer drug (DTX-SA-BSP). METHODS: Particle size, zeta potential and DTX release in vitro were measured in release media at different pH values. Quantitative cellular uptake, cytotoxicity assay in vitro and antitumour efficacy in vivo were also evaluated. Cell apoptosis was assessed by flow cytometry...
February 27, 2018: Journal of Pharmacy and Pharmacology
Xiaojing Lin, Tingbao Zhao, Cheng-Hsien Lin, Dan Zuo, Zhujun Ye, Shide Lin, Shaonan Wen, Lin Liu, Mao-Tsun Lin, Ching-Ping Chang, Chien-Ming Chao
OBJECTIVES: This study aimed to investigate the cardioprotective effects of melatonin on heat stroke (HS) induced acute myocardial infarction in rats and to explore the underlying mechanisms. METHODS: Myocardial injury was induced by subjecting the anaesthetized rats to a high ambient temperature of 43°C for 70 min. Such a high ambient temperature caused hyperthermia, hypotension and myocardial injury in rats. Rats were treated with melatonin (3 mg/kg) intravenously one hour before and followed by an additional dose immediately after heat stress...
February 27, 2018: Journal of Pharmacy and Pharmacology
Sandeep Rawat, Arun K Jugran, Indra D Bhatt, Ranbeer S Rawal
OBJECTIVES: Hedychium spicatum Buch. Ham. ex D.Don. (Family Zingiberaceae) is a rhizomatous herb, used in medicines, food, cosmetics and perfumery industries. Traditionally, it is widely used in treating inflammation, pain, asthma, foul breath, vomiting, diarrhoea, bronchitis, hiccough and blood diseases. This study systematically reviewed traditional and folk uses, pharmacological properties, bioactive compounds and market potential of H. spicatum. Research gaps and potential of future research have also been discussed...
February 27, 2018: Journal of Pharmacy and Pharmacology
Josiane M da Silva, Jéssica L M Conegundes, Nícolas C C Pinto, Renata F Mendes, Maria Christina M N Castañon, Elita Scio
OBJECTIVES: This study aimed to evaluate the chronic topical anti-inflammatory activity of the pharmaceutical formulation ProHLP containing the hexane fraction of Lacistema pubescens (HLP). It was also investigated the possible cutaneous and systemic adverse effects of HLP and ProHLP in mice when compared to dexamethasone. METHODS: The chronic topical anti-inflammatory activity was determined by croton oil multiple application-induced mouse ear oedema model. Histopathological analyses of ear tissue samples sensitized with croton oil were performed...
February 13, 2018: Journal of Pharmacy and Pharmacology
Apichaya Puangpetch, Weerapon Unaharassamee, Napa Jiratjintana, Napatrupron Koomdee, Chonlaphat Sukasem
OBJECTIVE: Single nucleotide polymorphisms in serotonin 2C receptor (HTR2C), leptin (LEP), and leptin receptor (LEPR) genes are reportedly associated with the presence of metabolic syndrome (MS). We investigated whether HTR2C:rs518147 (-697G/C), rs12836771 (A/G), LEP: rs7799039 (-2548G/A) and LEPR:rs1137101 (668A/G) are related to MS in psychotic disorder patients treated with atypical antipsychotics. METHODS: A cross-sectional study including 200 patients was conducted; genetic polymorphisms in HTR2C (rs518147 and rs12836771), LEP (rs7799039) and LEPR (rs1137101) were genotyped...
February 13, 2018: Journal of Pharmacy and Pharmacology
Harish Kumar, Rohini Agrawal, Vipin Kumar
OBJECTIVES: Barleria cristata (Family: Acanthaceae), commonly known as Philippine violet, is used in different ethnomedical systems for the treatment of a wide range of ailments. This review aimed to provide a scientific overview of B. cristata with reference to its ethnobotanical aspects, geographical distribution, medicinal uses, phytochemistry and pharmacological activity, and critical analyses research gaps and future research opportunities for investigations on this plant. KEY FINDINGS: Ethnomedical uses of the plant have been observed in lungs disorders, inflammatory conditions, toothache, anaemia, snake bite, diabetes and tuberculosis...
February 13, 2018: Journal of Pharmacy and Pharmacology
An-Kuo Chou, Chong-Chi Chiu, Ming-Ming Han, Yu-Wen Chen, Jhi-Joung Wang, Ching-Hsia Hung
OBJECTIVES: The objective of this experiment was to investigate spinal anaesthetic effects of pramoxine and its comparison with bupivacaine, a long-lasting local anaesthetic. METHODS: After intrathecal injection, three neurobehavioural assessments, which consisted of nociceptive, proprioceptive and motor block, were constructed in rats. The effects of bupivacaine and pramoxine (four doses of each drug) in a dose-related manner were conducted to obtain the ED 50 (50% effective dose)...
February 13, 2018: Journal of Pharmacy and Pharmacology
Ewa Witkowska-Banaszczak
OBJECTIVES: This article describes the flavonoid composition of the flowers of Trollius europaeus and the method of isolation thereof and provides an attempt at investigating the antioxidant activity of the isolated flavonoids and the antityrosinase activity of the extracts from the investigated material. METHODS: The compositional data were acquired by combining results of nuclear magnetic resonance, ultraviolet spectroscopy, electrospray ionisation-tandem mass spectrometry (ESI-MS/MS) analyses and those of an analysis of the products of acid hydrolysis of the compounds...
February 12, 2018: Journal of Pharmacy and Pharmacology
Hongxue Shen, Sheng Liu, Pinggang Ding, Lulu Wang, Jianming Ju, Guohui Liang
OBJECTIVES: We aimed to prepare novel magnolol-loaded mixed micelles (MAG-M) by pluronic F127 and L61 to overcome the challenges of magnolol's poor solubility and then further improve its oral bioavailability. METHODS: Magnolol-loaded mixed micelles containing pluronic F127 and L61 were prepared by an organic solvent evaporation method. Physicochemical, transport experiment across Caco-2 cell monolayers and pharmacokinetic studies were performed to characterize MAG-M and to determine the final improvement of the oral bioavailability...
February 12, 2018: Journal of Pharmacy and Pharmacology
Sun Young Park, Young-Whan Choi, Geuntae Park
OBJECTIVES: Our study verified the neuroprotective properties of emodin against oxygen-glucose deprivation/reoxygenation (OGD/R) and demonstrated its mechanism. METHODS: Human neuronal SH-SY5Y cells were investigated by analysing cell viability, lactate dehydrogenase levels, expression of molecules related to apoptotic cell death, and using biochemical techniques, flow cytometry and Western blot assays. KEY FINDINGS: Emodin reduced OGD/R-lead to neurotoxicity in SH-SY5Y cells...
February 9, 2018: Journal of Pharmacy and Pharmacology
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