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Life Sciences

Kyoung Hee Seo, Sun-Young Jung, Yeonsun Jin, Sung Hoon Lee
AIMS: Anoctamin-1 (TMEM16A) is a calcium-activated chloride channel that is involved in numerous physiological conditions. Its role has been identified in electrophysiological and histological studies of genetic knockout animals. Recent cellular localization studies have shown that anoctamin-1 is co-expressed with presynaptic proteins, therefore its role in presynaptic terminals has been suggested. However, behavioral studies are lacking because conventional knockouts of anoctamin-1 are lethal after birth...
June 18, 2018: Life Sciences
Zhi-Cheng Lai, Jin-Yu Liang, Li-Da Chen, Zhu Wang, Si-Min Ruan, Xiao-Yan Xie, Ming-De Lu, Hang-Tong Hu, Wei Wang
AIMS: To determine if tumors located in the subcapsular space or in proximity to vessels would be a risk factor of local tumor progression (LTP2 ) in hepatocellular carcinomas (HCC) after radiofrequency ablation. MATERIALS AND METHODS: A search of the MEDLINE, EMBASE and Cochrane Library databases from 1998 to 2017 was performed to identify studies examining the risk factors of LTP after radiofrequency ablation (RFA3 ) in HCC. No language or other restrictions were imposed...
June 17, 2018: Life Sciences
Hongfeng Zhang, Ling Li, Lei Liu
AIMS: Fcγ receptor I (FcγRI/CD64) that is restrictedly expressed on monocytes and macrophages, acts as the single high-affinity receptor of immunoglobulin G (IgG) in human. The expression of FcγRI is positively correlated with immune inflammation. The primary aim of this study was to explore the effects of FcγRI expression on immune-related inflammatory response and investigate the potential mechanisms. MAIN METHODS: FcγRI-expressing Ba/F3 cells are the ideal models for evaluating the functions of FcγRI...
June 16, 2018: Life Sciences
Abrar Al-Mahmood Siddiquee, P Ganesan Adaikan, Lang Chu Lau, Baharudin Bin Said, Mark Chong, Jerry Chan, Swee Hin Teoh
AIM: To investigate the effect of intracavernous injection of human umbilical cord blood derived endothelial colony forming cells (HUCB ECFCs) on erectile dysfunction (ED) in Zucker Diabetic Fatty (ZDF) rat model. METHODS: Erectile function was assessed by cavernous nerve electrostimulation in ZDF rats aged 20-28 weeks. Following confirmation of severe ED at the age of 28 weeks, 21 ZDF rats were randomly assigned to three experimental groups: 1 million ECFCs, 2 million ECFCs, and phosphate buffered saline (PBS)...
June 16, 2018: Life Sciences
Qi Tang, Peng Wu, Huiqing Chen, Guohui Li
Protein ubiquitination is a highly conserved post-translational modification affecting various biological processes including viral propagation. Ubiquitination has multiple effects on viral propagation, including viral genome uncoating, viral replication, and immune evasion. Ubiquitination of viral proteins is triggered by the ubiquitin-proteasome system (UPS). This involves the covalent attachment of the highly conserved 76 amino acid residue ubiquitin protein to target proteins by the consecutive actions of E1, E2 and E3 enzymes, and the 26S proteasome that together form a multiprotein complex that degrades target proteins...
June 15, 2018: Life Sciences
Hari Madhuri Doss, Snigdha Samarpita, Ramamoorthi Ganesan, Mahaboobkhan Rasool
AIMS: Bone erosion induced by enhanced osteoclast formation is a debilitating pathological phenomenon in rheumatoid arthritis (RA). Recent finding has revealed that ferulic acid is associated with reduced osteoclast differentiation and bone erosion. However, the underlying mechanism through which ferulic acid inhibited osteoclast differentiation and bone erosion still remains to be elucidated. This study assessed the therapeutic effects of ferulic acid on osteoclast differentiation and bone erosion by targeting RANKL dependent NF-κB pathway...
June 14, 2018: Life Sciences
Tarlan Varzandi, Mahammad Amin Abdollahifar, Seyed Ali Haeri Rohani, Abbas Piryaei, Azita Zadeh-Vakili, Sajad Jeddi, Asghar Ghasemi
INTRODUCTION: Nitric oxide (NO) deficiency is associated with obesity and type 2 diabetes. Nitrite, a NO donor, is considered as a new therapeutic agent in diabetes. This study aims at determining effects of long-term nitrite administration on browning of white adipose tissue (WAT) in type 2 diabetic rats. METHODS: Male rats were divided into 4 groups: Control, control + nitrite, diabetes, and diabetes + nitrite. Sodium nitrite (50 mg/L in drinking water) was administered for 3 months...
June 10, 2018: Life Sciences
Xi Zhang, Yifan Feng, Yuping Wang, Jing Wang, Danni Xiang, Weiran Niu, Fei Yuan
AIMS: To explore the roles of mitochondrial biogenesis and dynamics in both RGC-5 cells apoptosis and rat retinal damage induced by elevated pressure and their involvement in resveratrol (RSV)-induced cell protection. MATERIALS AND METHODS: The chronic ocular hypertension (COH) model was established in rats by injecting superparamagnetic iron oxide into anterior chamber. The RGC-5 cells were incubated under ambient and elevated pressure (70 mm Hg) respectively...
June 9, 2018: Life Sciences
Jiayi Zhang, Yang Liu, Laijin Lu
Peripheral nerve injury is one of the most common clinical diseases. Although the regeneration of the peripheral nerve is better than that of the nerves of the central nervous system, because of its growth rate restrictions after damage. Hence, the outcome of repair after injury is not favorable. Small RNA, a type of non-coding RNA, has recently been gaining attention in neural injury. It is widely distributed in the nervous system in vivo and a significant change in the expression of small RNAs has been observed in a neural injury model...
June 9, 2018: Life Sciences
Yongling Guo, Qin Fang, Dan Ma, Kunling Yu, Bingqing Cheng, Sishi Tang, Tingting Lu, Weili Wang, Jishi Wang
PURPOSE: HDAC4/5 and Smad7 are potential therapeutic targets for the onset and progression of B-cell acute lymphocytic leukemia (B-ALL) and indices for clinical prognosis. In contrast, HO-1 (heat shock protein 32) plays a key role in protecting tumor cells from apoptosis. METHODS: HDAC4/5, HO-1 and Smad7 expressions in 34 newly diagnosed B-ALL cases were detected by real-time PCR and Western blot. Lentivirus and small interference RNA were used to transfect B-ALL cells...
June 7, 2018: Life Sciences
Nosrat Bahrami, Saeed Mehrzadi, Mehdi Goudarzi, Esrafil Mansouri, Iman Fatemi
Di (2-ethylhexyl) phthalate (DEHP) is one of the environmental pollutants that causes testicular damage. Lycopene (LYCO), the main active carotenoid in red fruits and vegetables, has well-known antiinflammatory and antioxidant activities. The present study aimed to investigate the effects of LYCO on DEHP-induced testicular injury in male mice. DEHP (2 g/kg, p.o.) was given for two weeks to mice. LYCO was given at 4 mg/kg, p.o., for two weeks, starting the same day of DEHP insult. Serum testosterone, luteinizing hormone and follicle stimulating hormone and testicular total antioxidant status, malondialdehyde, nitric oxide, glutathione, superoxide dismutase, glutathione peroxidase, catalase, tumor necrosis factor-α, interleukin-1β were measured...
June 7, 2018: Life Sciences
Nahla E El-Ashmawy, Eman G Khedr, El-Zeiny M Ebeid, Mohamed L Salem, Esraa M Mosalam, Abdel-Aziz A Zidan
AIMS: This study aimed to elucidate the benefits of nanoformulation of doxorubicin (DOX) and thymoquinone (TQ) loaded with nanofibers of poly-N-acetyl glucosamine (pGlcNAc), which is known as F2 gel, over their conventional free forms. Moreover, evaluate the role of TQ in improving chemotherapeutic effect and ameliorating nephrotoxicity of DOX. MAIN METHODS: The drugs were loaded into F2 gel followed by measurement of physicochemical characterization. Next, MCF-7 and HEPG2 cells were treated with the prepared formulations and assessed for apoptosis alongside with cellular proliferation...
June 6, 2018: Life Sciences
Ying Hui, Yan Yin
Diabetic retinopathy (DR) remains a leading cause of blindness in adults with diabetes mellitus. The pathogenesis of DR is a multifactorial process, and excessive oxidative stress and apoptosis in retinal endothelial cells (RECs) play vital roles. In this study, RECs were treated with 25 mM glucose for three days, and we found that miR-145 was significantly downregulated in high glucose (HG)-treated RECs. Overexpression of miR-145 attenuated the HG-induced apoptosis, oxidative stress and inflammatory cytokines secretion in RECs...
June 5, 2018: Life Sciences
Shuli Fan, Xiang Gao, Peng Chen, Xu Li
Myricetin has been reported to promote osteogenic differentiation and inhibit osteoclastogenesis. The aim of this study was to investigate the potential effects of myricetin on glucocorticoid-induced osteoporosis (GIOP) both in vivo and in vitro. Male Sprague-Dawley (SD) rats were given dexamethasone (DEX, 0.1 mg/kg, s.c.) once daily and myricetin (1 mg/kg or 2.5 mg/kg, i.p.) once every other day for a total of five weeks. Body weight was recorded once a week. Bone mineral density (BMD), the activities or levels of bone turnover markers, and histological changes were assessed...
June 5, 2018: Life Sciences
Hsiu-Chuan Chou, Chieh-Hsiang Lu, Yi-Ching Su, Li-Hsun Lin, Hui-I Yu, Hsiang-Hsun Chuang, Yi-Ting Tsai, En-Chi Liao, Yu-Shan Wei, Yi-Ting Yang, Yu-An Chien, Xin-Ru Yu, Ying-Ray Lee, Hong-Lin Chan
AIMS: Honokiol is a natural product extracted from herbal plants such as the Magnolia species which have been shown to exhibit anti-tumor and anti-metastatic properties. However, the effects of honokiol on thyroid cancers are largely unknown. MATERIALS AND METHODS: To determine whether honokiol might be useful for the treatment of thyroid cancer and to elucidate the mechanism of toxicity of honokiol, we analyzed the impact of honokiol treatment on differential protein expression in human thyroid cancer cell line ARO using lysine-labeling two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS)...
June 5, 2018: Life Sciences
Zohreh Nowzari, Mohammad Masoumi, Mahdieh Nazari-Robati, Hamed Akbari, Nader Shahrokhi, Gholamreza Asadikaram
AIMS: Apelin and leptin are factors which have a potential physiological and pathological role in cardiovascular homoeostasis. Apelin receptor (APLNR), leptin receptor (LEPR) and leptin variants may affect the vascular tone in heart or peripheral circulation, thereby predisposing patients to hypertension and coronary artery disease (CAD). The aim of the present study was to evaluate four single nucleotide polymorphisms (SNPs) of APLNR genes (rs11544374 and rs948847), LEPR (rs1137101) and leptin (rs7799039) gene in patients with CAD and hypertension...
June 5, 2018: Life Sciences
Elba V M M Carvalho, Weslley F Oliveira, Luana C B B Coelho, Maria T S Correia
Lectins are carbohydrate binding proteins that can stimulate cell proliferation. This property makes these biomolecules capable of being used as mitogen reagents to study the interaction with lymphocytes allowing evaluation of immunomodulatory action, since B and T lymphocytes are related to humoral and innate immunity, respectively. Isolated cells from spleen, which include lymphocytes, are widely applied as a model in screening lectin mitogenic capacity. This mitotic stimulus is initiated by interaction of the lectin with T-cell receptor on cell surface...
June 4, 2018: Life Sciences
Yu Fei, Jianhua Hou, Wei Xuan, Chenghua Zhang, Xiuping Meng
OBJECTIVE: Although angiogenesis plays an important role in coronary collateral circulation (CCC) formation and there are many determinants of coronary angiogenesis, they cannot fully explain the mechanism of CCC formation or as potent biomarker for CCC status. Therefore, there is of great clinical significance to identify the novel molecules associated with CCC. Previously, miR-503 exerts anti-angiogenesis effect via inhibition of VEGF-A and its expression is associated with many angiogenesis-related factors...
June 2, 2018: Life Sciences
Bruna Romana-Souza, Jeanine Salles Dos Santos, Andréa Monte-Alto-Costa
AIMS: In pressure ulcers, the synthesis of reactive oxygen species induced by ischemia and reperfusion leads to chronic inflammation and tissue damage, which impair the closure of these lesions. Caffeic acid phenethyl ester (CAPE), found in propolis, promotes cutaneous wound healing of acute lesions and severe burns. However, the effects of CAPE on wound healing of pressure ulcers have not been investigated. This study investigated the effects of CAPE administration in a murine model of pressure ulcers...
June 1, 2018: Life Sciences
Raisa Petrović, Biljana Bufan, Nevena Arsenović-Ranin, Irena Živković, Rajna Minić, Katarina Radojević, Gordana Leposavić
AIMS: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). MAIN METHODS: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-γ and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively...
May 31, 2018: Life Sciences
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