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Journal of Laboratory and Clinical Medicine

M Teresa Santos, Juana Vallés, Justo Aznar, Aida Lago, Elena Sanchez, Juan Cosin, Antonio Moscardó, Marta Piñón, M Johan Broekman, Aaron J Marcus
Inhibition of erythrocyte (RBC) promotion of platelet reactivity could improve the antiplatelet effect of aspirin (ASA). We tested different ASA regimens for optimal inhibition of platelets and the effects of RBC in patients with a history of vascular diseases. Collagen-induced platelet activation (14C-5HT, TXA2 release) and platelet recruitment (proaggregatory activity of cell-free releasates from activated platelets) were measured in PRP, platelet-RBC (Hct 40%), and whole blood (WB) in 206 patients initially on 200-300-mg ASA/day...
May 2006: Journal of Laboratory and Clinical Medicine
Margo P Cohen, Fuad N Ziyadeh, Sheldon Chen
No abstract text is available yet for this article.
May 2006: Journal of Laboratory and Clinical Medicine
Amanda F Baker, Tomislav Dragovich, Wendy R Tate, Ramesh K Ramanathan, Denise Roe, Chiu-Hsieh Hsu, D Lynn Kirkpatrick, Garth Powis
Thioredoxin-1 (Trx-1) is a small redox protein that is overexpressed in many human tumors, where it is associated with aggressive tumor growth and decreased patient survival. Trx-1 is secreted by tumor cells and is present at increased levels in the plasma of cancer patients. PX-12 is an irreversible inhibitor of Trx-1 currently in clinical development as an antitumor agent. We have used SELDI-TOF mass spectroscopy to measure plasma Trx-1 from patients treated with PX-12 during a phase I study. Mean plasma Trx-1 levels at pretreatment were significantly elevated in the cancer patients at 182...
February 2006: Journal of Laboratory and Clinical Medicine
Gabriella Doronzo, Isabella Russo, Luigi Mattiello, Mariella Trovati, Giovanni Anfossi
The C-reactive protein (CRP) is a strong predictor of cardiovascular events both in the general population and in patients with coronary artery disease. We aimed to evaluate whether in cultured human vascular smooth muscle cells (hVSMC) CRP modulates the synthesis and release of metalloproteinase-2 (MMP-2), which is deeply involved in plaque instabilization and vascular remodeling, and of the tissue inhibitor of metalloproteinase-2 (TIMP-2). Both in supernatants and in cell lysates of cultured hVSMC exposed to CRP (0-10 mg/L), we evaluated MMP-2 activity (gelatin zymography), MMP-2 and TIMP-2 protein synthesis (immunoblotting), MMP-2 and TIMP-2 mRNA expression (reverse transcription-polymerase chain reaction)...
November 2005: Journal of Laboratory and Clinical Medicine
Gerold Holzer, Helge Noske, Thomas Lang, Lukas Holzer, Ulrike Willinger
We sought to evaluate serum concentrations of cathepsin K in peripheral blood and to determine whether they correlated with bone-mineral density (BMD) and the incidence of nontraumatic fractures. We took blood samples from 162 patients (101 with osteoporosis, 48 with osteopenia) and 13 healthy controls, then conducted quantitative measurements of cathepsin K using a commercially available enzyme-linked immunosorbent assay. Cathepsin K concentrations were correlated with the incidence of nontraumatic fracture, BMD, markers of bone turnover (alkaline phosphatase, bone-specific alkaline phosphatase, osteocalcin, parathyroid hormone, and C-telopeptide)...
July 2005: Journal of Laboratory and Clinical Medicine
Solomon S Solomon, Nicholas Buss, James Shull, Susanne Monnier, Gipsy Majumdar, Jian Wu, Ivan C Gerling
Insulin resistance may be modeled in H-411E liver cells in tissue culture with the use of the cytokine tumor necrosis factor-alpha (TNF-alpha) and insulin. This tissue-culture model nicely mimics IR in human type 2 diabetes mellitus. After incubation of liver cells in tissue culture with INS alone, TNF-alpha alone, and TNF-alpha plus insulin, as well as a control sample, liver-cell extracts were separated on 2D polyacrylamide-gel electrophoresis on the basis of isoelectric point and molecular weight. We analyzed the gel images with the use of PD Quest software (Bio-Rad Laboratories, Hercules, Calif) to identify differentially expressed protein spots (ie, up or down with insulin vs down or up with TNF-alpha plus insulin)...
May 2005: Journal of Laboratory and Clinical Medicine
Gregory E Kobak, Rolf Dahl, Michael W Devereaux, Eric Gumpricht, Maret Traber, R Brian Doctor, Ronald J Sokol
UNLABELLED: Metabolic liver disorders cause chronic liver disease and liver failure in childhood. Many of these disorders share the histologic features of steatosis and cholestasis, or steatocholestasis. In this study we sought to (1) develop an in vitro model of steatocholestasis, (2) determine the mechanisms of cell death in this model, and (3) determine the role of mitochondrial disturbances in this model. METHODS: Hepatocytes were isolated from 8-week-old obese (fa/fa) and lean Zucker rats...
May 2005: Journal of Laboratory and Clinical Medicine
Kazuhiro Okano, Kosaku Nitta, Shuhei Ogawa, Shigeru Horita, Katsuyoshi Habiro, Hiroshi Nihei, Ryo Abe
Inducible costimulator (ICOS) is the third member of the CD28 superfamily, expressed on antigen-primed T-cells, enhancing Th2 differentiation. Anti-glomerular basement membrane (anti-GBM) glomerulonephritis results from multiple effects generated by both Th1 and Th2 cells. To evaluate the contribution of these T-cells to the progression of anti-GBM glomerulonephritis, we investigated the effect of double blockade of CD28 and ICOS signaling. Anti-GBM glomerulonephritis was induced in C57BL/6 mice, a Th1-prone strain...
October 2004: Journal of Laboratory and Clinical Medicine
Miyazaki Kenichi, Miyazaki Masanobu, Koji Takehiko, Tsukasaki Shoko, Furusu Akira, Abe Katsushige, Harada Takashi, Ozono Yoshiyuki, Kohno Shigeru
Plasmin is an important factor in the degradation of extracellular matrix. In the study reported here we examined the expression of plasminogen-activator inhibitor-1 (PAI-1), urokinase-type plasminogen activator (uPA), and uPA receptor (uPAR), as well as the relevance of such expression to the production of type IV collagen, a major component of extracellular matrix, in the renal tissue of rats with streptozotocin-induced diabetes. Because angiotensin II is involved in the synthesis of PAI-1 and uPA, we also examined the effect of benazepril, an angiotensin-converting-enzyme inhibitor, on the expression of PAI-1, uPA, and uPAR messenger RNAs (mRNAs) and type IV collagen protein...
August 2004: Journal of Laboratory and Clinical Medicine
Lucia Terzuoli, Roberto Pagani, Barbara Frosi, Alessandra Galli, Cosetta Felici, Lucio Barabesi, Brunetta Porcelli
No abstract text is available yet for this article.
January 2004: Journal of Laboratory and Clinical Medicine
Atsuko Kamijo, Kenjiro Kimura, Takeshi Sugaya, Masaya Yamanouchi, Akihisa Hikawa, Norihito Hirano, Yasunobu Hirata, Atsuo Goto, Masao Omata
Previous studies have indicated that in massive proteinuria, free fatty acids (FFAs) bound to albumin were overloaded in the proximal tubule and exacerbated tubulointerstitial damage. Liver-type fatty acid-binding protein (L-FABP) is an intracellular carrier protein of FFAs that is expressed in the proximal tubule of human kidney. We sought to evaluate urinary L-FABP as a clinical marker in chronic renal disease. Urinary L-FABP was measured in patients with nondiabetic chronic renal disease (n = 120) with the use of a newly established ELISA method...
January 2004: Journal of Laboratory and Clinical Medicine
Hongjun Zhang, Tat-Kin Tsang, Christy A Jack
Endoscopic biliary stenting is a common treatment for the palliation of obstructive jaundice caused by inoperable malignant hepatobiliary tumors and benign strictures. The biliary stent, however, often becomes nonfunctional as a result of occlusion. In this study, we sought to confirm that bile glycoprotein mucin was a factor in stent occlusion and to investigate its possible role in biliary-stent blockage. The high-molecular-weight glycoprotein fraction was isolated from stent sludge with the use of gel filtration and a cesium chloride density gradient...
July 2003: Journal of Laboratory and Clinical Medicine
Hiroyuki Hori, Hirohisa Nakata, Genzo Iguchi, Hajime Yamada, Kazuo Chihara, Hisamitsu Baba
Gastrin has the ability to stimulate cell growth in some colorectal cancer cells and some of these cells also express gastrin/CCKB receptors, suggesting that gastrin and its autocrine loop are involved in their proliferation. We previously reported that oncogenic ras induced gastrin gene expression in colon cancer cells. The aim of this study was to investigate whether oncogenic ras also induces gastrin/CCKB receptor gene expression. A transiently transfected activated ras vector stimulated gastrin/CCKB receptor transcriptional activities in both Colo320HSR and LoVo cells, but these ras-increased activities were inhibited by a specific MEK inhibitor, PD98059...
May 2003: Journal of Laboratory and Clinical Medicine
N Yasemin Oguz
Research ethics committees (RECs) are the institutional equivalent of institutional review boards (IRBs) with respect to their role in the ethical analysis of research projects. In Turkey, as in most developing countries, these committees were initiated as a result of pressure from the Western scientific community. Since their formation, RECs have faced problems because of an underdeveloped scientific culture and the absence of established ethical standards. On the one hand, the standards of the RECs and IRBs in the developed world seemed too hard to achieve; on the other, some of the international regulatory rules, such as respect for autonomy, are not culturally sensitive...
May 2003: Journal of Laboratory and Clinical Medicine
Matthew M Heeney, Thad A Howard, Sherri A Zimmerman, Russell E Ware
Hydroxyurea therapy reduces hemolysis and decreases serum bilirubin levels in children and adults with sickle cell anemia (SCA) and may therefore help prevent the development of cholelithiasis in this patient population. We recently reported that a promoter polymorphism in the uridine diphosphoglucuronate glucuronosyltransferase 1A (UGT1A) gene affects steady-state bilirubin levels and the incidence of gallstones in children with SCA. We have now analyzed the influence of the UGT1A genotype on the therapeutic response to hydroxyurea...
April 2003: Journal of Laboratory and Clinical Medicine
Margo P Cohen, Elizabeth Shea, Sheldon Chen, Clyde W Shearman
Albumin modified by Amadori glucose adducts has been shown to modulate signal transduction and induce alterations in renal glomerular cells that contribute to the development of diabetic nephropathy. However, the participation of this nonenzymatically glycated protein in the pathobiology of atherosclerotic cardiovascular disease in diabetes has not been established. To probe this issue, we used macrophage RAW cells to assess the effects of glycated albumin on molecular events implicated in the pathogenesis of diabetes-related vascular complications...
April 2003: Journal of Laboratory and Clinical Medicine
Axel Stachon, Nina Sondermann, Matthias Imohl, Michael Krieg
The presence of nucleated red blood cells (NRBCs) in the blood of patients with a variety of severe diseases is known to be highly associated with increased mortality. Blood analyzers to routinely measure NRBC concentrations are now available. However, the prognostic significance of such measurements has not been evaluated. Using a Sysmex XE-2100 (Sysmex; Norderstedt, Germany), we measured NRBC concentrations in 15,541 blood samples from 4,173 patients at a university clinic over the course of 12 weeks. NRBCs were found at least once in 7...
December 2002: Journal of Laboratory and Clinical Medicine
Richard F Branda, Zhuan Chen, Elice M Brooks, Shelly J Naud, Thomas D Trainer, John J McCormack
The effects of diet and folate status on cyclophosphamide or 5-fluorouracil toxicity were studied in Fischer 344 rats maintained on either a cereal-based diet or a purified diet (AIN-93G). The rats fed the purified diet were divided into 3 groups: folate deficient (no dietary folic acid), folate replete (2 mg folic acid/kg diet), and high folate (2 mg folic acid/kg diet plus 50 mg/kg body weight folic acid intraperitoneally daily). The LD50 for cyclophosphamide was significantly higher for the cereal diet than for the purified diets, but there was no difference among the purified diets...
November 2002: Journal of Laboratory and Clinical Medicine
Alex V Birk, M Johan Broekman, Eva M Gladek, Hugh D Robertson, Joan H F Drosopoulos, Aaron J Marcus, Hazel H Szeto
Extracellular adenosine triphosphate (ATP) regulates platelet reactivity by way of direct action on platelet purinergic receptors or by hydrolysis to adenosine diphosphate (ADP). Subsequent metabolism of ATP and ADP to adenosine monophosphate (AMP) and adenosine inhibits platelet aggregation. Endothelial cell membrane-bound ecto-ATP/ADPase (CD39, E-NTPDase1) is thought to be the main regulator of platelet responsiveness. However, the findings in studies of CD39-knockout mice imply that nucleotidase(s) in plasma regulates circulating adenine nucleotides levels...
September 2002: Journal of Laboratory and Clinical Medicine
Takekazu Kaji, Shunji Ishihara, Nobuo Ashizawa, Naoharu Hamamoto, Hiroshi Endo, Ryo Fukuda, Kyoichi Adachi, Makoto Watanabe, Masafumi Nakao, Yoshikazu Kinoshita
Adherence of Helicobacter pylori to the gastric epithelium is believed to be an important step in the induction of active inflammation of the mucosal layer. However, structural evidence showing a quantitative relationship between the adherence of H. pylori and severity of gastric mucosal inflammation is lacking. We therefore investigated the correlations between severity of gastritis and adherence of morphologically different forms of H. pylori. Fifty-seven biopsy specimens from the gastric bodies of patients with H...
April 2002: Journal of Laboratory and Clinical Medicine
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