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Journal of Laboratory and Clinical Medicine

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https://www.readbyqxmd.com/read/16697771/coordinate-activation-of-intracellular-signaling-pathways-by-insulin-like-growth-factor-1-and-platelet-derived-growth-factor-in-rat-hepatic-stellate-cells
#1
Kim R Bridle, Lin Li, Rosemary O'Neill, Robert S Britton, Bruce R Bacon
Proliferation of activated hepatic stellate cells (HSC) is an important event in the development of hepatic fibrosis. Insulin-like growth factor-1 (IGF-1) has been shown to be mitogenic for HSC, but the intracellular signaling pathways involved have not been fully characterized. Thus, the aims of the current study were to examine the roles of the extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase (PI3-K) and p70-S6 kinase (p70-S6-K) signaling pathways in IGF-1- and platelet-derived growth factor (PDGF)-induced mitogenic signaling of HSC and to examine the potential crosstalk between these pathways...
May 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16697769/aspirin-therapy-for-inhibition-of-platelet-reactivity-in-the-presence-of-erythrocytes-in-patients-with-vascular-disease
#2
M Teresa Santos, Juana Vallés, Justo Aznar, Aida Lago, Elena Sanchez, Juan Cosin, Antonio Moscardó, Marta Piñón, M Johan Broekman, Aaron J Marcus
Inhibition of erythrocyte (RBC) promotion of platelet reactivity could improve the antiplatelet effect of aspirin (ASA). We tested different ASA regimens for optimal inhibition of platelets and the effects of RBC in patients with a history of vascular diseases. Collagen-induced platelet activation (14C-5HT, TXA2 release) and platelet recruitment (proaggregatory activity of cell-free releasates from activated platelets) were measured in PRP, platelet-RBC (Hct 40%), and whole blood (WB) in 206 patients initially on 200-300-mg ASA/day...
May 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16697768/amadori-modified-glycated-serum-proteins-and-accelerated-atherosclerosis-in-diabetes-pathogenic-and-therapeutic-implications
#3
REVIEW
Margo P Cohen, Fuad N Ziyadeh, Sheldon Chen
No abstract text is available yet for this article.
May 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16581349/rudolf-virchow
#4
Dale E Hammerschmidt
No abstract text is available yet for this article.
April 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16503246/long-term-effect-of-heme-oxygenase-ho-1-induction-in-glomerular-immune-injury
#5
Prasun K Datta, Pu Duann, Elias A Lianos
In a rat model of macrophage-dependent glomerular immune injury induced by administration of antibody against the glomerular basement membrane (anti-GBM), the authors assessed the anti-proteinuric effect of Heme Oxygenase-1 (HO-1) induction. Rats received anti-GBM antibody alone, anti-GBM antibody and treatment with the HO-1 inducer, hemin, or non-immune serum (controls). Urine protein, creatinine, and nitrite/nitrate excretion were measured on days 5, 7, and 14 after administration of the anti-GBM antibody...
March 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16459169/a-restless-investigator
#6
Dale E Hammerschmidt
No abstract text is available yet for this article.
February 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16459168/identifying-type-vicenza-von-willebrand-disease
#7
Alessandra Casonato, Elena Pontara, Francesca Sartorello, Maria Grazia Cattini, Lisa Gallinaro, Antonella Bertomoro, Antonio Rosato, Roberto Padrini, Antonio Pagnan
Increased clearance of von Willebrand factor (VWF) is one of the main features of type Vicenza von Willebrand disease (VWD), a variant with plasma and platelet VWF level discrepancies and unusually large VWF multimers. Diagnosing type Vicenza VWD may not be easy, due to its heterogeneous phenotype. Here we describe the criteria we adopted to identify type Vicenza in a large group of VWD patients. Emphasizing the contribution of platelet VWF by comparison with plasma values, a first step involved selecting the candidate Vicenza patients on the basis of low or very low plasma VWF and a normal platelet VWF content...
February 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16459166/the-antitumor-thioredoxin-1-inhibitor-px-12-1-methylpropyl-2-imidazolyl-disulfide-decreases-thioredoxin-1-and-vegf-levels-in-cancer-patient-plasma
#8
Amanda F Baker, Tomislav Dragovich, Wendy R Tate, Ramesh K Ramanathan, Denise Roe, Chiu-Hsieh Hsu, D Lynn Kirkpatrick, Garth Powis
Thioredoxin-1 (Trx-1) is a small redox protein that is overexpressed in many human tumors, where it is associated with aggressive tumor growth and decreased patient survival. Trx-1 is secreted by tumor cells and is present at increased levels in the plasma of cancer patients. PX-12 is an irreversible inhibitor of Trx-1 currently in clinical development as an antitumor agent. We have used SELDI-TOF mass spectroscopy to measure plasma Trx-1 from patients treated with PX-12 during a phase I study. Mean plasma Trx-1 levels at pretreatment were significantly elevated in the cancer patients at 182...
February 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16442999/hgba1c-and-glucose-control-we-cannot-play-it-straight-with-patients
#9
EDITORIAL
Byron J Hoogwerf
No abstract text is available yet for this article.
January 2006: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16310518/serum-levels-of-c-reactive-protein-and-procalcitonin-in-critically-ill-patients-with-cirrhosis-of-the-liver
#10
Daliana Peres Bota, Marc Van Nuffelen, Ahmed N Zakariah, Jean-Louis Vincent
Concentrations of C-reactive protein (CRP) and procalcitonin (PCT) have been suggested as markers of infection. The liver is believed to be a key source of CRP and PCT. For this reason we assessed the predictive value of these markers in patients with hepatic cirrhosis in a 31-bed university-hospital department of intensive care. Demographic, clinical, laboratory, and microbiologic data were collected prospectively over 9 months. Of 864 patients included in the study, 79 (9%) had hepatic cirrhosis. Patients with cirrhosis were more likely to have a medical than a surgical admission diagnosis (67 vs 47%, P = ...
December 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16310515/differential-regulation-of-the-rat-heme-oxygenase-1-expression-by-ets-oncoproteins-in-glomerular-mesangial-cells
#11
Prasad D K Dhulipala, Prasun K Datta, E Shyam Reddy, Elias A Lianos
The Ets-1 oncoprotein and the heme-catabolizing enzyme heme oxygenase (HO)-1 have been implicated in the pathogenesis of renal disease. We investigated the role of the putative Ets-binding sites (EBSs) in the transactivation of the proximal promoter of rat heme oxygenase 1 (hmox1) gene by the Ets oncoproteins Fli-1, Erg-2, and Ets-1 in mesangial cells. We examined several rat hmox1-chloramphenicol acetytransferase (CAT) constructs and EBS mutant constructs in an effort to assess the effect of ETS oncoproteins on transactivation of the rat hmox1 proximal promoter in renal glomerular mesangial cells...
December 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16242531/about-the-cover-illustration
#12
EDITORIAL
Dale E Hammerschmidt
No abstract text is available yet for this article.
November 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16242528/c-reactive-protein-increases-matrix-metalloproteinase-2-expression-and-activity-in-cultured-human-vascular-smooth-muscle-cells
#13
Gabriella Doronzo, Isabella Russo, Luigi Mattiello, Mariella Trovati, Giovanni Anfossi
The C-reactive protein (CRP) is a strong predictor of cardiovascular events both in the general population and in patients with coronary artery disease. We aimed to evaluate whether in cultured human vascular smooth muscle cells (hVSMC) CRP modulates the synthesis and release of metalloproteinase-2 (MMP-2), which is deeply involved in plaque instabilization and vascular remodeling, and of the tissue inhibitor of metalloproteinase-2 (TIMP-2). Both in supernatants and in cell lysates of cultured hVSMC exposed to CRP (0-10 mg/L), we evaluated MMP-2 activity (gelatin zymography), MMP-2 and TIMP-2 protein synthesis (immunoblotting), MMP-2 and TIMP-2 mRNA expression (reverse transcription-polymerase chain reaction)...
November 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16131453/reminiscences-of-dr-cecil-j-watson
#14
EDITORIAL
Rudi Schmid
No abstract text is available yet for this article.
September 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/16025086/soluble-cathepsin-k-a-novel-marker-for-the-prediction-of-nontraumatic-fractures
#15
Gerold Holzer, Helge Noske, Thomas Lang, Lukas Holzer, Ulrike Willinger
We sought to evaluate serum concentrations of cathepsin K in peripheral blood and to determine whether they correlated with bone-mineral density (BMD) and the incidence of nontraumatic fractures. We took blood samples from 162 patients (101 with osteoporosis, 48 with osteopenia) and 13 healthy controls, then conducted quantitative measurements of cathepsin K using a commercially available enzyme-linked immunosorbent assay. Cathepsin K concentrations were correlated with the incidence of nontraumatic fracture, BMD, markers of bone turnover (alkaline phosphatase, bone-specific alkaline phosphatase, osteocalcin, parathyroid hormone, and C-telopeptide)...
July 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/15902099/proteome-of-h-411e-liver-cells-exposed-to-insulin-and-tumor-necrosis-factor-alpha-analysis-of-proteins-involved-in-insulin-resistance
#16
Solomon S Solomon, Nicholas Buss, James Shull, Susanne Monnier, Gipsy Majumdar, Jian Wu, Ivan C Gerling
Insulin resistance may be modeled in H-411E liver cells in tissue culture with the use of the cytokine tumor necrosis factor-alpha (TNF-alpha) and insulin. This tissue-culture model nicely mimics IR in human type 2 diabetes mellitus. After incubation of liver cells in tissue culture with INS alone, TNF-alpha alone, and TNF-alpha plus insulin, as well as a control sample, liver-cell extracts were separated on 2D polyacrylamide-gel electrophoresis on the basis of isoelectric point and molecular weight. We analyzed the gel images with the use of PD Quest software (Bio-Rad Laboratories, Hercules, Calif) to identify differentially expressed protein spots (ie, up or down with insulin vs down or up with TNF-alpha plus insulin)...
May 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/15902097/increased-susceptibility-of-fat-laden-zucker-rat-hepatocytes-to-bile-acid-induced-oncotic-necrosis-an-in-vitro-model-of-steatocholestasis
#17
Gregory E Kobak, Rolf Dahl, Michael W Devereaux, Eric Gumpricht, Maret Traber, R Brian Doctor, Ronald J Sokol
UNLABELLED: Metabolic liver disorders cause chronic liver disease and liver failure in childhood. Many of these disorders share the histologic features of steatosis and cholestasis, or steatocholestasis. In this study we sought to (1) develop an in vitro model of steatocholestasis, (2) determine the mechanisms of cell death in this model, and (3) determine the role of mitochondrial disturbances in this model. METHODS: Hepatocytes were isolated from 8-week-old obese (fa/fa) and lean Zucker rats...
May 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/15871309/kibuka-s-umbilical-cord
#18
Dale E Hammerschmidt
No abstract text is available yet for this article.
March 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/15754469/polycystic-ovaries
#19
Dale E Hammerschmidt
No abstract text is available yet for this article.
February 2005: Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/15746654/regulation-of-alternative-splicing-of-caspase-2-through-an-intracellular-signaling-pathway-in-response-to-pro-apoptotic-stimuli
#20
Nozomi Iwanaga, Makoto Kamachi, Kouichiro Aratake, Yasumori Izumi, Hiroaki Ida, Fumiko Tanaka, Mami Tamai, Kazuhiko Arima, Hideki Nakamura, Tomoki Origuchi, Atsushi Kawakami, Katsumi Eguchi
Alternative splicing is an important mechanism in the generation of functionally distinct products from the same gene. Some apoptosis-regulating genes also undergo alternative splicing, generating splice variants that antagonzie normal transcripts on apoptosis. For example, caspase-2 is alternatively spliced, leading to exon 9-lacking caspase-2L (proapoptotic) and exon 9-containing caspase-2S (antiapoptotic) transcripts. Serine-arginine splicing factor proteins (SR proteins) are highly conserved and required for constitutive and alternative messenger RNA (mRNA) splicing...
February 2005: Journal of Laboratory and Clinical Medicine
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