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Indian Journal of Microbiology

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https://www.readbyqxmd.com/read/29434408/infection-and-microbiome-impact-of-tuberculosis-on-human-gut-microbiome-of-indian-cohort
#1
Utkarsh Sood, Abhay Bajaj, Roshan Kumar, Sachin Khurana, Vipin Chandra Kalia
No abstract text is available yet for this article.
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434407/carotenoid-production-by-a-novel-isolate-of-microbacterium-paraoxydans
#2
Swati Ojha, Sumeet Kapoor, Saroj Mishra
This study reports extraction and characterization of carotenoid pigments from Microbacterium paraoxydans, a non-photosynthetic bacterium, cultivated in Luria-Bertani (LB) medium. The isolate was identified to be moderately halo- and osmo-tolerant capable of withstanding high (~ 6%) salt and sugar (30% w/v sucrose, 20% w/v glucose) concentrations after a brief period of adaptation. The pigments were characterized using a combination of UV-Vis spectral analysis with the λmax at 407, 436 and 466 nm and ESI-MS with an m/z value at 536...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434406/phage-application-for-the-protection-from-acute-hepatopancreatic-necrosis-disease-ahpnd-in-penaeus-vannamei
#3
Jin Woo Jun, Jee Eun Han, Sib Sankar Giri, Kathy F J Tang, Xiaohui Zhou, Luis Fernando Aranguren, Hyoun Joong Kim, Saekil Yun, Cheng Chi, Sang Guen Kim, Se Chang Park
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has been one of the most problematic diseases in marine shrimp aquaculture throughout Southeast Asia and Latin America. To evaluate the effectiveness of a bacteriophage (phage) treatment for AHPND, a series of bioassays were carried out in a marine shrimp (Penaeus vannamei) model using an AHPND-V. parahaemolyticus strain that is highly pathogenic to shrimp. We monitored the mortality and histopathological changes during phage treatment...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434405/inclusion-of-2-mercaptoethanol-in-lysis-buffer-could-interfere-with-isolation-of-high-molecular-weight-dna-from-freshwater-microalgae
#4
Willy Yee, Janushree Nanda Kumar, Priya Devi Muthusamy
2-mercaptoethanol (2-ME), alongside polyvinylpyrrolidone is commonly used in plant DNA extractions to deal with polyphenols, which could interfere with extraction and downstream applications. 2-ME is also commonly used to denature proteins and nucleases, especially RNAses. On the contrary, we found that the presence of 2-ME in lysis buffer interfered with DNA extraction from 12 strains of freshwater microalgae, resulting in DNA with poor integrity. We also found that the TNES-urea buffer, commonly used for preservation and DNA extraction from fish, appears as effective as the SDS and CTAB buffer for some microalgae strains...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434404/in-vitro-antibacterial-activity-of-phlorotannins-from-edible-brown-algae-eisenia-bicyclis-against-streptomycin-resistant-listeria-monocytogenes
#5
Hyo-Jung Kim, Chakradhar Dasagrandhi, Song-Hee Kim, Bo-Geum Kim, Sung-Hwan Eom, Young-Mog Kim
Listeria monocytogenes (LM) is an important food borne pathogen responsible for listeriosis. Further, LM is an etiological agent associated with life threatening conditions like meningitis and encephalitis. Biofilm forming and drug resistant LM may potentially become difficult to treat infections and hence effective controlling measures are required to prevent LM infections. In view of this, the present study evaluated an anti-listerial potential of edible brown seaweed, Eisenia bicyclis, by disc diffusion and micro-dilution methods...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434403/synthesis-of-protein-inorganic-nanohybrids-with-improved-catalytic-properties-using-co3-po4-2
#6
Ashok Kumar, In-Won Kim, Sanjay K S Patel, Jung-Kul Lee
In the present study, a method for easy and rapid synthesis of lipase nanohybrids was evaluated using cobalt chloride as an encapsulating agent. The synthesized nanohybrids exhibited higher activity (181%) compared to free lipase and improved catalytic properties at higher temperature and in harsh conditions. The nanohybrids retained 84% of their residual activity at 25 °C after 10 days. In addition, these nanohybrids also exhibited high storage stability and reusability. Collectively, the synthesis of carrier-free immobilized biocatalysts was performed rapidly within 24 h at 4 °C...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434402/synthesis-antimicrobial-and-antioxidant-activity-of-pyrazole-based-sulfonamide-derivatives
#7
Jagdish R Badgujar, Dhananjay H More, Jyotsna S Meshram
A series of new sulfonamides have been synthesized from Ampyrone with different benzene sulfonyl chlorides to yield the N-1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl) benzenesulfonamides (4a-e). All synthesized compounds were characterized on the basis of FTIR, 1H NMR, and 13C NMR, and also by the aid of mass spectral data. Further, all synthesized compounds have studied for their in vitro antimicrobial activities against selected bacterial as well as fungal strains by the agar well diffusion method...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434401/point-of-care-tuberculosis-sero-diagnosis-kit-for-wild-animals-combination-of-proteins-for-improving-the-diagnostic-sensitivity-and-specificity
#8
Maroudam Veerasami, K Venkataraman, Chitra Karuppannan, Arun Attur Shanmugam, Mallepaddi Chand Prudhvi, Thomas Holder, Polavarapu Rathnagiri, K Arunmozhivarman, Gopal Dhinakar Raj, Martin Vordermeier, B Mohana Subramanian
Tuberculosis is a significant problem globally for domestic animals as well as captive and free ranging wild life. Rapid point of care (POC) serology kits are well suited for the diagnosis of TB in wild animals. However, wild animals are invariably exposed to environmental non-pathogenic mycobacterium species with the development of cross reacting antibodies. In the present study, POC TB diagnosis kit was developed using a combination of pathogenic Mycobacteria specific recombinant antigens and purified protein derivatives of pathogenic and non-pathogenic Mycobacteria...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434400/evaluation-of-gamma-radiation-inactivation-of-a-bioterrorism-agent-bacillus-anthracis-spores-on-different-materials
#9
Mesut Ortatatli, Kadir Canitez, Sermet Sezigen, Ruşen Koray Eyison, Levent Kenar
Decontamination of suspected packages, such as sealed envelopes, liquids and tools that are likely contaminated with biological agents is of great importance. In this study, we aimed to determine the gamma radiation dose required for the decontamination of paper, fabric and liquid materials without causing any damage to the structure of these materials. Each study group included 11 pieces of paper, fabric and sterile saline contaminated with 0.8 × 105 virulent Bacillus anthracis (B. anthracis) spores. These specimens were exposed to doses of 5...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434399/anti-viral-activities-of-oroxylum-indicum-extracts-on-chikungunya-virus-infection
#10
Syuhadaratul Aini Mohamat, Rafidah Hanim Shueb, Nor Fazila Che Mat
Chikungunya virus (CHIKV) is a re-emerging mosquito-borne alphavirus that poses a threat to human worldwide. Driven by the lack of approved medication and vaccination, research on anti-Chikungunya agents has received great attention. In an effort to determine potential inhibitor of CHIKV, this study aimed at investigating the potential anti-viral activity of Oroxylum indicum extracts towards CHIKV-infected Vero cells. The virucidal, pre- and post-treatment effects of O. indicum were evaluated, using the maximum non-toxic dose of O...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434398/a-highly-promiscuous-integron-plasmids-extended-spectrum-beta-lactamases-and-efflux-pumps-as-factors-governing-multidrug-resistance-in-a-highly-drug-resistant-vibrio-fluvialis-isolate-bd146-from-kolkata-india
#11
Neha Rajpara, Mrinalini Nair, Ashima Kushwaha Bhardwaj
In an earlier study from this laboratory, Vibrio fluvialis BD146, a clinical isolate from Kolkata, India, 2002, was found to be resistant to all the fourteen antibiotics tested. It harboured a high copy number plasmid pBD146 and a low copy number plasmid. In the present study, a more detailed analysis was carried out to unravel different resistance mechanisms in this isolate. Sequencing showed that variable region of class 1 integron located on low copy number plasmid harbored arr3-cmlA-blaOXA10-aadA1 gene cassettes...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434397/evaluation-of-industrial-biocides-on-a-novel-aspergillus-versicolor-tanck-1-and-elucidation-of-their-probable-biocidal-mechanism
#12
S Kavitha, Yasmin Khambhaty, N K Chandra Babu
Abstract: In the present investigation, a novel fungus was isolated from leather watch strap and identified as Aspergillus versicolor TANCK-1 by 18 s rRNA sequencing. The isolated fungus was evaluated against three structurally different fungicides such as 2-(thiocyanomethylthio)benzothiazole (TCMTB), potassium dimethyldithiocarbamate (KDDC) and 2,2-dibromo-3-nitrilopropionamide (DBNP) to control the fungal growth. Among these, TCMTB was found to effectively inhibit the growth at a minimum concentration of 31...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434396/structural-and-functional-properties-of-exopolysaccharide-excreted-by-a-novel-bacillus-anthracis-strain-pfab2-of-hot-spring-origin
#13
Aparna Banerjee, Shalini Gaur Rudra, Koushik Mazumder, Vinod Nigam, Rajib Bandopadhyay
Exopolysaccharide produced by a unique avirulent Bacillus anthracis strain PFAB2 of hot spring origin has been characterized and its functional properties are investigated which is a first report. Maximum yield of EPS is 7.66 g/l with 2% glucose and 1% peptone as optimum carbon and nitrogen source respectively. The EPS is found to be a homopolymer consisting of only glucose as principle monosaccharide component. Through 1H NMR study, different dextran-like proton peaks are observed. Molecular weight of the EPS resembles low molecular weight bacterial origin polysaccharides...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434395/co-utilization-of-crude-glycerol-and-biowastes-for-producing-polyhydroxyalkanoates
#14
Subhasree Ray, Rakesh Sharma, Vipin Chandra Kalia
Polyhydroxyalkanoate (PHA) production by Bacillus thuringiensis EGU45 and defined mixed culture of Bacillus spp. were studied by using crude glycerol (CG) and hydrolyzed biowastes as feed material. Hydrolysates from onion peels (OP), potato peels, pea-shells (PS), apple pomace 2% total solids obtained with defined mixed hydrolytic cultures (MHC2) were inoculated with B. thuringiensis EGU45 and defined mixed bacterial cultures (5MC1), which produced PHA at the rate of 40-350 and 65-450 mg/L, respectively. Addition of CG (1%, v/v) to these hydrolysates resulted in 1...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434394/mycobacterium-mageritense-parotitis-in-an-immunocompetent-adult
#15
Taro Okabe, Teppei Sasahara, Jun Suzuki, Tsubasa Onishi, Masayoshi Komura, Shigehiro Hagiwara, Hiromichi Suzuki, Yuji Morisawa
Mycobacterium mageritense, a rapidly growing mycobacterium, is a rare clinical pathogen. Furthermore, parotitis due to non-tuberculosis mycobacterium is very rare in adults. Herein, we report the first case of M. mageritense parotitis in an immunocompetent adult. A 40-year-old man presented with swelling in a left parotid lesion. He was diagnosed with parotitis. The culture from the parotid abscess grew M. mageritense. He was unsuccessfully treated with levofloxacin monotherapy. Trimethoprim-sulfamethoxazole was added, leading to some clinical response; however, the erythema persisted despite 14 months of antibiotic therapy...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434393/the-mycotoxin-zearalenone-hinders-candida-albicans-biofilm-formation-and-hyphal-morphogenesis
#16
Satish Kumar Rajasekharan, Jin-Hyung Lee, Yueju Zhao, Jintae Lee
Yeast-mold mycobiota inhabit several natural ecosystems, in which symbiotic relationships drive strategic pathoadaptation. Mycotoxins are metabolites produced by diverse mycotoxigenic fungi as a defense against yeasts, though at times yeasts secrete enzymes that degrade, detoxify, or bio-transform mycotoxins. The present study is focused on the in vitro inhibitory effects of zearalenone (ZEN), a F2 mycotoxin produced by several Fusarium and Gibberella species, on different microbial strains. ZEN exhibited no effect on the planktonic growth or biofilms of several Gram positive and negative bacteria at the tested concentrations...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434392/nanoparticles-in-biological-hydrogen-production-an-overview
#17
Sanjay K S Patel, Jung-Kul Lee, Vipin C Kalia
Biological hydrogen (H2) production enhancement through the use of nanoparticles (NPs) supplement in the media is being recognized as a promising approach. The NPs, including those of metal and metal oxides have shown a significant improvement in the BHP. A number of organisms as pure or mixed cultures can produce H2 in presence of NPs from pure sugars and biowaste as a feed. However, their H2 production efficiencies have been found to vary significantly with the type of NPs and their concentration. In this review article, the potential role of NPs in the enhancement of H2 production has been assessed in dark- and photo-fermentative organisms using sugars and biowaste materials as feed...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434391/gut-bioreactor-and-human-health-in-future
#18
Hemant J Purohit
Gut-microbiome provides the complementary metabolic potential to the human system. To understand the active participation and the performance of the microbial community in human health, the concept of gut as a plug-flow reactor with the fed-batch mode of operation can provide better insight. The concept suggests the virtual compartmentalized gut with sequential stratification of the microbial community in response to a typical host genotype. It also provides the analysis plan for gut microbiome; and its relevance in developing health management options under the identified clinical conditions...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434390/the-torch-bearer-microbiologist-prof-j-jay-vakil-1927-2017
#19
EDITORIAL
V K Rale, V C Kalia
No abstract text is available yet for this article.
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29151654/development-and-evaluation-of-simple-dot-blot-assays-for-rapid-detection-of-staphylococcal-enterotoxin-a-in-food
#20
Mamta Singh, Ravi Kant Agrawal, Bhoj Raj Singh, Sanjod Kumar Mendiratta, Rajesh Kumar Agarwal, Mithilesh Kumar Singh, Deepak Kumar
The present study was aimed to develop and evaluate dot-blot assays for rapid detection of staphylococcal enterotoxin-A (SEA) in food. Dot blots were developed in two formats, indirect and sandwich utilizing mouse monoclonal anti-SEA and rabbit polyclonal anti-SEA antibodies. In indirect dot-blot format, recombinant SEA was directly coated on NCM dot-blot strip and detection was carried out by anti-SEA antibodies. In sandwich dot-blot format, SEA was trapped between anti-SEA capture and detection antibodies...
December 2017: Indian Journal of Microbiology
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