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Indian Journal of Microbiology

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https://www.readbyqxmd.com/read/29651188/integrated-artificial-intelligence-approaches-for-disease-diagnostics
#1
Rajat Vashistha, Deepak Chhabra, Pratyoosh Shukla
Mechanocomputational techniques in conjunction with artificial intelligence (AI) are revolutionizing the interpretations of the crucial information from the medical data and converting it into optimized and organized information for diagnostics. It is possible due to valuable perfection in artificial intelligence, computer aided diagnostics, virtual assistant, robotic surgery, augmented reality and genome editing (based on AI) technologies. Such techniques are serving as the products for diagnosing emerging microbial or non microbial diseases...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651187/effect-of-encapsulation-on-viability-of-bifidobacterium-longum-cfr815j-and-physiochemical-properties-of-ice-cream
#2
Ankita Kataria, Sajan C Achi, Prakash M Halami
The health beneficial attributes of bifidobacteria and its safe association with the host gut has increased its significance as a probiotic. However delivering probiotic bifidobacteria with Minimum Biological Value (MBV) through product has always been a challenge. In the present study, an attempt was made to maintain the viability of native isolate of Bifidobacterium longum CFR 815j and deliver through ice-cream. B. longum CFR815j was microencapsulated in alginate starch capsules by emulsification followed by evaluation of bead stability in simulated gastrointestinal conditions...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651186/in-vitro-activity-of-doxycycline-and-%C3%AE-lactam-combinations-against-different-strains-of-burkholderia-pseudomallei
#3
Nurul Iman Mohamad, Azian Harun, Habsah Hasan, Zakuan Zainy Deris
Although doxycycline is active against Burkholderia pseudomallei and has been used in the eradication stage of melioidosis therapy, it is not regularly used during the initial intensive phase. In order to assess its potential use in intensive phase therapy, we investigated in vitro pharmacodynamic activity of doxycycline and β-lactams alone and in combination against four Malaysian strains of B. pseudomallei. Using a checkerboard assay, the combinations of doxycycline and imipenem, doxycycline and ceftazidime, and doxycycline and amoxicillin-clavulanate tested against four strains showed indifferent effects with summation fractional inhibitory concentration values ranging from 0...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651185/tackling-salmonella-persister-cells-by-antibiotic-nisin-combination-via-mannitol
#4
Praveen Rishi, Neha Rani Bhagat, Reena Thakur, Preeti Pathania
Bacterial persisters (defined as dormant, non-dividing cells with globally reduced metabolism) are the major cause of recurrent infections. As they neither grow nor die in presence of antibiotics, it is difficult to eradicate these cells using antibiotics, even at higher concentrations. Reports of metabolites (which help in waking up of these inactive cells) enabled eradication of bacterial persistence by aminoglycosides, suggest the new potential strategy to improve antibiotic therapy. Here we propose, mannitol enabled elimination of Salmonella persister cells by the nisin-antibiotic combination...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651184/psychrotolerant-sphingobacterium-kitahiroshimense-lt-2-isolated-from-dhundi-glacier-himachal-pradesh-origin-prediction-and-future-application
#5
Shivika Sharma, Subhankar Chatterjee
A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651183/annotation-and-de-novo-sequence-characterization-of-extracellular-%C3%AE-fructofuranosidase-from-penicillium-chrysogenum-strain-hkf42
#6
Vaibhav V Gujar, Priya Fuke, Anshuman A Khardenavis, Hemant J Purohit
The genome of a fungal strain Penicillium chrysogenum strain HKF42, which can grow on 20% sucrose has been annotated for 7595 protein coding sequences. On mining of CAZymes, we could annotate a β-fructofuranosidase gene responsible for fructo-oligosaccharides (FOS) synthesis which is a known prebiotic. The enzyme activity was demonstrated and validated with the generation of FOS as kestose and nystose.
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651182/first-report-of-a-new-isolate-of-metarhizium-rileyi-from-maize-fields-of-quivic%C3%A3-n-cuba
#7
Sandra Pérez Álvarez, Amaury Méndez Guerrero, Bernardo Nayar Débora Duarte, Marco Antonio Magallanes Tapia, Jesús Alicia Chávez Medina, Yoannis Domínguez Rodríguez
Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S . frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651181/extracellular-synthesis-and-characterization-of-gold-nanoparticles-using-mycobacterium-sp-brs2a-ar2-isolated-from-the-aerial-roots-of-the-ghanaian-mangrove-plant-rhizophora-racemosa
#8
Mustafa Camas, Anil Sazak Camas, Kwaku Kyeremeh
Through the use of genomes that have undergone millions of years of evolution, marine Actinobacteria are known to have adapted to rapidly changing environmental pressures. The result is a huge chemical and biological diversity among marine Actinobacteria . It is gradually becoming a known fact that, marine Actinobacteria have the capability to produce nanoparticles which have reasonable sizes and structures with possible applications in biotechnology and pharmacology. Mycobacterium sp. BRS2A-AR2 was isolated from the aerial roots of the mangrove plant Rhizophora racemosa ...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651180/production-of-a-mixture-of-fengycins-with-surfactant-and-antifungal-activities-by-bacillus-sp-ma04-a-versatile-pgpr
#9
Alejandro Hernández-Morales, Ramón-Alvar Martínez-Peniche, Jackeline-Lizzeta Arvizu-Gómez, Sofía-María Arvizu-Medrano, Areli Rodríguez-Ontiveros, Miguel-Angel Ramos-López, Juan-Ramiro Pacheco-Aguilar
Bacillus sp. strain MA04 a plant growth-promoting rhizobacteria (PGPR) showed hemolytic activity on blood agar plates, and the supernatant from liquid culture in nutrient broth at 24 h exhibited emulsification activity, suggesting the production of biosurfactants. In antagonist assays, the supernatant showed antifungal activity against phytopathogenic fungi such as Penicillium expansum , Fusarium stilboides , Sclerotium rolfsii y Rhizoctonia solani , finding a reduction of mycelial growth of all fungi tested, ranging from 35 to 69%, this activity was increased with time of culture, accomplishing percentages of inhibition up to 85% with supernatants obtained at 72 h...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651179/incubation-of-aquilaria-subintegra-with-microbial-culture-supernatants-enhances-production-of-volatile-compounds-and-improves-quality-of-agarwood-oil
#10
Sakon Monggoot, Chadin Kulsing, Yong Foo Wong, Patcharee Pripdeevech
Incubation with microbial culture supernatants improved essential oil yield from Aquilaria subintegra woodchips. The harvested woodchips were incubated with de man, rogosa and sharpe (MRS) agar, yeast mold (YM) agar medium and six different microbial culture supernatants obtained from Lactobacillus bulgaricus , L. acidophilus , Streptococcus thermophilus , Lactococcus lactis , Saccharomyces carlsbergensis and S. cerevisiae prior to hydrodistillation. Incubation with lactic acid bacteria supernatants provided higher yield of agarwood oil (0...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651178/transformation-products-of-carbamazepine-cbz-after-ozonation-and-their-toxicity-evaluation-using-pseudomonas-sp-strain-ksh-1-in-aqueous-matrices
#11
Kshitiz Dwivedi, Ashwinkumar P Rudrashetti, Tapan Chakrabarti, R A Pandey
Carbamazepine (CBZ) is an anti-epileptic and anti-convulsant drug widely used for the treatment of epilepsy and other bipolar disorders. Ozone as an advanced oxidation process has been widely used for the degradation of CBZ resulting in the formation of transformation products (ozonides). The present research aims to isolate and identify potential microorganism, capable of degradation of CBZ and its transformation products. The cell viability and cytotoxicity of pure CBZ and their ozone transformation products were evaluated using the cells of Pseudomonas sp...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651177/potential-of-marine-derived-fungi-to-remove-hexavalent-chromium-pollutant-from-culture-broth
#12
Nikita P Lotlikar, Samir R Damare, Ram Murti Meena, P Linsy, Brenda Mascarenhas
Chromium (Cr) released from industrial units such as tanneries, textile and electroplating industries is detrimental to the surrounding ecosystems and human health. The focus of the present study was to check the Cr(VI) removal efficiency by marine-derived fungi from liquid broth. Amongst the three Cr(VI) tolerant isolates, #NIOSN-SK56-S19 ( Aspergillus sydowii ) showed Cr-removal efficiency of 0.01 mg Cr mg-1 biomass resulting in 26% abatement of total Cr with just 2.8 mg of biomass produced during the growth in 300 ppm Cr(VI)...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651176/cloning-of-ompa-gene-from-acinetobacter-baumannii-into-the-eukaryotic-expression-vector-pbudce4-1-as-dna-vaccine
#13
Hossein Ansari, Abbas Doosti, Mohammad Kargar, Mahdi Bijanzadeh, Mojtaba Jaafarinia
Antibiotic resistant features of Acinetobacter baumannii is partly due to the decreased outer membrane proteins (OMPs) permeability. The OmpA is one of the most conserved proteins among A. baumannii with a considerable antigenic potential to stimulate the multidimensional immune system responses. The present study was aimed to clone the ompA gene into the eukaryotic expression vector with potential as DNA vaccine. The ompA gene of A. baumannii was amplified using polymerase chain reaction (PCR). The target DNA was cloned and sub-cloned into the pTZ57R/T and pBudCE4...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651175/adaptive-strategies-of-bacillus-thuringiensis-isolated-from-acid-mine-drainage-site-in-sabah-malaysia
#14
Low Yi Yik, Grace Joy Wei Lie Chin, Cahyo Budiman, Collin Glenn Joseph, Baba Musta, Kenneth Francis Rodrigues
The adaptive process in bacteria is driven by specific genetic elements which regulate phenotypic characteristics such as tolerance to high metal ion concentrations and the secretion of protective biofilms. Extreme environments such as those associated with heavy metal pollution and extremes of acidity offer opportunities to study the adaptive mechanisms of microorganisms. This study focused on the genome analysis of Bacillus thuringiensis (Bt MCMY1), a gram positive rod shaped bacterium isolated from an acid mine drainage site in Sabah, Malaysia by using a combination of Single Molecule Real Time DNA Sequencing, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR)...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651174/identification-and-pathogenicity-of-fungal-pathogens-causing-black-point-in-wheat-on-the-north-china-plain
#15
Kai-Ge Xu, Yu-Mei Jiang, Yang-Kun Li, Qiao-Qiao Xu, Ji-Shan Niu, Xin-Xin Zhu, Qiao-Yun Li
Fungi associated with black point were isolated from three highly susceptible wheat genotypes in the North China Plain. The 21 isolates represented 11 fungal genera. The most prevalent genera were Alternaria (isolation frequency of 56.7%), Bipolaris (16.1%), and Fusarium (6.0%). The other eight genera were Curvularia , Aspergillus , Cladosporium , Exserohilum , Epicoccum , Nigrospora , Penicillium , and Ulocladium ; their isolation frequencies ranged from 0.8 to 4.8%. The pathogenicity of the isolates was individually assessed in the greenhouse by inoculating wheat plants with spore suspensions...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651173/in-vitro-anticancer-activity-of-staphyloxanthin-pigment-extracted-from-staphylococcus-gallinarum-kx912244-a-gut-microbe-of-bombyx-mori
#16
Delicia Avilla Barretto, Shyam Kumar Vootla
The present study reports the in vitro biological nature of the pigment produced by Staphylococcus gallinarum KX912244, isolated as the gut microflora bacterium of the insect Bombyx mori . The purified pigment was characterized as Staphyloxanthin based on bio-physical characterization techniques like Fourier transform infrared spectroscopy, high performance liquid chromatography, Proton nuclear magnetic resonance spectroscopy (1 H NMR), Liquid chromatography-Mass spectroscopy and Gas chromatography-Mass spectroscopy...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651172/virulence-factors-in-candida-albicans-and-streptococcus-mutans-biofilms-mediated-by-farnesol
#17
Renan Aparecido Fernandes, Douglas Roberto Monteiro, Laís Salomão Arias, Gabriela Lopes Fernandes, Alberto Carlos Botazzo Delbem, Debora Barros Barbosa
The aim of this study was to evaluate the effect of farnesol on the production of acids and hydrolytic enzymes by biofilms of Streptococcus mutans and Candida albicans . The present study also evaluated the time-kill curve and the effect of farnesol on matrix composition and structure of single-species and dual-species biofilms. Farnesol, at subinhibitory concentrations, showed a significant reduction in S. mutans biofilm acid production, but did not alter C. albicans hydrolytic enzyme production. The number of cultivable cells of both microorganisms was significantly reduced after 8 h of contact with farnesol...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651171/wastewater-a-potential-bioenergy-resource
#18
Jyotsana Prakash, Rakesh Sharma, Subhasree Ray, Shikha Koul, Vipin Chandra Kalia
Wastewaters are a rich source of nutrients for microorganisms. However, if left unattended the biodegradation may lead to severe environmental hazards. The wastewaters can thus be utilized for the production of various value added products including bioenergy (H2 and CH4 ). A number of studies have reported utilization of various wastewaters for energy production. Depending on the nature of the wastewater, different reactor configurations, wastewater and inoculum pretreatments, co-substrate utilizations along with other process parameters have been studied for efficient product formation...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434408/infection-and-microbiome-impact-of-tuberculosis-on-human-gut-microbiome-of-indian-cohort
#19
Utkarsh Sood, Abhay Bajaj, Roshan Kumar, Sachin Khurana, Vipin Chandra Kalia
No abstract text is available yet for this article.
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29434407/carotenoid-production-by-a-novel-isolate-of-microbacterium-paraoxydans
#20
Swati Ojha, Sumeet Kapoor, Saroj Mishra
This study reports extraction and characterization of carotenoid pigments from Microbacterium paraoxydans , a non-photosynthetic bacterium, cultivated in Luria-Bertani (LB) medium. The isolate was identified to be moderately halo- and osmo-tolerant capable of withstanding high (~ 6%) salt and sugar (30% w/v sucrose, 20% w/v glucose) concentrations after a brief period of adaptation. The pigments were characterized using a combination of UV-Vis spectral analysis with the λmax at 407, 436 and 466 nm and ESI-MS with an m/z value at 536...
March 2018: Indian Journal of Microbiology
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