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Experimental Cell Research

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https://www.readbyqxmd.com/read/28432002/mir-429-inhibits-metastasis-by-targeting-kiaa0101-in-soft-tissue-sarcoma
#1
Devyani Samantarrai, Bibekanand Mallick
Soft tissue sarcomas (STS) are a heterogeneous group of rare tumors with high metastatic potential. There being only a handful of publication on metastasis of STS, we investigated the miRNA mediated target gene regulations in modulating the metastatic processes in this cancer. In this study, we amalgamated gene and miRNA expression profiles of high-grade STS samples with miRNA target predictions and identified miR-429 targeting KIAA0101 as a novel pair, which remain unexplored in STS metastasis. We validated their expression in metastatic fibrosarcoma cell line, HT1080 and performed several functional assays using miRNA mimics and KIAA0101 over-expression vector to confirm their role in metastasis...
April 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28432001/in-vivo-and-in-vitro-study-of-osteogenic-potency-of-endothelin-1-on-bone-marrow-derived-mesenchymal-stem-cells
#2
Long-Wei Hu, Xiao Wang, Xin-Qun Jiang, Li-Qun Xu, Hong-Ya Pan
Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration. Endothelin-1(EDN-1)is produced by vascular endothelial cells which plays an important role during bone development. However, its role in BMSCs remains largely unknown. We established EDN-1 overexpressed BMSCs, proliferation ability and osteogenesis differentiation were detected respectively...
April 18, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412247/activation-of-phospholipase-c-%C3%AE-1-and-translocation-of-phosphatidylinositol-3-4-5-trisphosphate-3-phosphatase-contribute-to-gl-v9-induced-apoptosis-in-human-gastric-cancer-cells
#3
Kai Zhao, Guojun Li, Yuyuan Yao, Yuxin Zhou, Zhiyu Li, Qinglong Guo, Na Lu
Gastric cancer is the most common type of tumor in developing countries and the fourth most frequently diagnosed cancer worldwide. Here, we demonstrated the apoptotic effects of GL-V9 on several human gastric cancer cells and selected MGC-803 cells to uncover the underlying mechanism. GL-V9 elevated Bax/Bcl-2 ratio, abated mitochondrial membrane potential and triggered the onset of apoptotic execution in MGC-803 cells. Our research revealed that CHOP silencing could not inhibit apoptosis, neither could it block Ca(2+) release, suggesting that GL-V9-induced apoptosis was independent of CHOP...
April 13, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412246/absence-of-nucks-augments-paracrine-effects-of-mesenchymal-stem-cells-mediated-cardiac-protection
#4
Yuelin Zhang, Sinming Chiu, Xiaoting Liang, Yuet-Hung Chai, Yiming Qin, Junwen Wang, Xiang Li, Beiying Qiu, Vinay Tergaonkar, Hung-Fat Tse, Qizhou Lian
Bone marrow-derived mesenchymal stem cells (BM-MSCs) contribute to myocardial repair after myocardial infarction (MI) by secreting a panel of growth factors and cytokines. This study was to investigate the potential mechanisms of the nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS) in regulation of the profiles of BM-MSCs secretion and compare the therapeutic efficacy of NUCKS(-/-)- and wide type-BM-MSCs (WT-BM-MSCs) on MI. The secretion profiles between NUCKS(-/-)- and WT-BM-MSCs under hypoxia (1%O2) were analyzed...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412245/cxcr5-cd8-t-cells-infiltrate-the-colorectal-tumors-and-nearby-lymph-nodes-and-are-associated-with-enhanced-igg-response-in-b-cells
#5
Junjie Xing, Chenxin Zhang, Xiaohong Yang, Shaoxuan Wang, Zhongchuan Wang, Xu Li, Enda Yu
Colorectal cancer is the third most prevalent cancer type worldwide and contributes to a significant percentage of cancer-related mortality. Recent studies have shown that the CXCR5(+)CD8(+) T cells present more potent proinflammatory function than CXCR5(-)CD8(+) T cells in chronic virus infections and in follicular lymphoma, but the role of CXCR5(+)CD8(+) T cells in colorectal cancer is yet unclear. In this study, we demonstrated that CXCR5(+)CD8(+) T cells were very rare in peripheral blood mononuclear cells from healthy and colorectal cancer individuals, but were significantly enriched in resected tumors and tumor-associated lymph nodes...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412244/a-central-role-for-cadherin-signaling-in-cancer
#6
REVIEW
Antonis Kourtidis, Ruifeng Lu, Lindy J Pence, Panos Z Anastasiadis
Cadherins are homophilic adhesion molecules with important functions in cell-cell adhesion, tissue morphogenesis, and cancer. In epithelial cells, E-cadherin accumulates at areas of cell-cell contact, coalesces into macromolecular complexes to form the adherens junctions (AJs), and associates via accessory partners with a subcortical ring of actin to form the apical zonula adherens (ZA). As a master regulator of the epithelial phenotype, E-cadherin is essential for the overall maintenance and homeostasis of polarized epithelial monolayers...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412243/macrophage-conditioned-medium-induced-cellular-network-formation-in-mcf-7-cells-through-enhanced-tunneling-nanotube-formation-and-tunneling-nanotube-mediated-release-of-viable-cytoplasmic-fragments
#7
Pooja Patheja, Khageswar Sahu
Infiltrating macrophages in tumor microenvironment, through their secreted cytokines and growth factors, regulate several processes of cancer progression such as cancer cell survival, proliferation, invasion, metastasis and angiogenesis. Recently, intercellular cytoplasmic bridges between cancer cells referred as tunneling nanotubes (TNTs) have been recognized as novel mode of intercellular communication between cancer cells. In this study, we investigated the effect of inflammatory mediators present in conditioned medium derived from macrophages on the formation of TNTs in breast adenocarcinoma cells MCF-7...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412242/14-3-3%C3%AE-regulates-copine1-mediated-neuronal-differentiation-in-hib5-hippocampal-progenitor-cells
#8
Jae Cheal Yoo, Nammi Park, Boah Lee, Abdullateef Nashed, Young-Sun Lee, Tae Hwan Kim, Da Yong Lee, Ajung Kim, Eun Mi Hwang, Gwan-Su Yi, Jae-Yong Park
Copine1 (CPNE1), known as a calcium-dependent membrane-binding protein, has tandem C2 domains and an A domain. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Protein kinase B (AKT) phosphorylation in the hippocampal progenitor cell line, HiB5. To better understand its cellular function, we carried out a yeast two-hybrid screening to find CPNE1 binding partners. Among the identified proteins, 14-3-3γ appears to directly interact with CPNE1. Between CPNE1 and 14-3-3γ, the physical interaction as well as the specific binding regions of CPNE1 was confirmed in vitro and in vivo...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28412241/cathepsin-l-silencing-increases-as2o3-toxicity-in-malignantly-transformed-pilocytic-astrocytoma-mpa58-cells-by-activating-caspases-3-7
#9
Monika Primon, Peter C Huszthy, Helena Motaln, Krishna M Talasila, Hrvoje Miletic, Nadia A Atai, Rolf Bjerkvig, Tamara Lah Turnšek
Low-grade, pilocytic astrocytomas are treated by resection, but additional therapy is necessary for those tumors with anaplastic features. Arsenic trioxide (As2O3) is emerging as an effective chemotherapeutic agent for treatment of malignant glioblastoma multiforme, where Cathepsin L silencing enables lower, less harmful As2O3 concentrations to achieve the desired cytotoxic effect. Here, we evaluated the effects of As2O3 combined with stable Cathepsin L shRNA silencing on cell viability/metabolic activity, and apoptosis in primary cultures of recurrent malignantly transformed pilocytic astrocytoma (MPA)...
April 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28408318/regulation-of-agr2-expression-via-3-utr-shortening
#10
Eva Matoulkova, Lucia Sommerova, Michal Pastorek, Borivoj Vojtesek, Roman Hrstka
One recently discussed general mechanism affecting gene expression is 3'-untranslated region (3'UTR) length. Events such as shortening, translocation or loss of 3'UTRs are observed within oncogenes and are proposed to associate with increased expression. Thus, increased efforts are being made to understand constitutive and differential transcript 3'end formation. Investigation of AGR2 mRNA revealed a direct impact of its 3'UTR length on AGR2 expression. In silico analyses identified several regulatory sequences within the distal part of AGR2 mRNA that may regulate 3'UTR length and associated protein levels...
April 11, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28408319/deriving-and-testing-of-dysplastic-murine-hepatocytes-a-new-platform-in-liver-cancer-research
#11
Sharon Pok, Harpreet Vohra, Charbel Wehbe, Vanessa A Barn, Evi Arfianti, Yock-Young Dan, Geoffrey C Farrell, Narci C Teoh
Dysplastic hepatocytes(DH) represent altered hepatocytes with potential for malignant transformation. To date, most research on pathways to hepatocarcinogenesis has focused on use of "hepatoma" cell-lines derived from hepatocellular carcinoma(HCC). We describe a novel technique for deriving/culturing DH and demonstrate their utility for functional studies in vitro, compared to primary hepatocytes(PH) and HCC. PH and DH were prepared by portal vein collagenase perfusion from C57BL/6J mice. DH were subsequently subjected to FACS...
April 10, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28408317/involvement-of-puma-in-pericyte-migration-induced-by-methamphetamine
#12
Yanhong Zhang, Yuan Zhang, Ying Bai, Jie Chao, Gang Hu, Xufeng Chen, Honghong Yao
Mounting evidence indicates that methamphetamine causes blood-brain barrier damage, with emphasis on endothelial cells. The role of pericytes in methamphetamine-induced BBB damage remains unknown. Our study demonstrated that methamphetamine increased the migration of pericytes from the endothelial basement membrane. However, the detailed mechanisms underlying this process remain poorly understood. Thus, we examined the molecular mechanisms involved in methamphetamine-induced pericyte migration. The results showed that exposure of C3H/10T1/2 cells and HBVPs to methamphetamine increased PUMA expression via activation of the sigma-1 receptor, MAPK and Akt/PI3K pathways...
April 10, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28408316/down-regulation-of-lrp1b-in-colon-cancer-promoted-the-growth-and-migration-of-cancer-cells
#13
Zhiqiang Wang, Peng Sun, Chun Gao, Ji Chen, Jun Li, Zhonghao Chen, Ming Xu, Jun Shao, Yunpeng Zhang, Jiang Xie
Aberrant activation of beta-catenin/TCF signaling is one of the hallmarks of colon cancer. It is of great interest to study the mechanism for the regulation of beta-catenin/TCF signaling. In this study, it was found that LRP1B was down-regulated in colon cancer tissues and inhibited the growth, migration and metastasis of colon cancer cells. The molecular mechanism study revealed that LRP1B interacted with DVL2, inhibited the interaction between DVL2 and Axin, and negatively regulated beta-catenin/TCF signaling...
April 10, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28392353/decreased-nuclear-stiffness-via-fak-erk1-2-signaling-is-necessary-for-osteopontin-promoted-migration-of-bone-marrow-derived-mesenchymal-stem-cells
#14
Lingling Liu, Qing Luo, Jinghui Sun, Aoli Wang, Yisong Shi, Yang Ju, Yasuyuki Morita, Guanbin Song
Migration of bone marrow-derived mesenchymal stem cells (BMSCs) plays an important role in many physiological and pathological settings, including wound healing. During the migration of BMSCs through interstitial tissues, the movement of the nucleus must be coordinated with the cytoskeletal dynamics, which in turn affects the cell migration efficiency. Our previous study indicated that osteopontin (OPN) significantly promotes the migration of rat BMSCs. However, the nuclear behaviors and involved molecular mechanisms in OPN-mediated BMSC migration are largely unclear...
April 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28392352/cellular-recognition-and-patterning-in-sensory-systems
#15
REVIEW
Hideru Togashi, Sayaka Katsunuma
Cells dissociated from various tissues of vertebrate embryos preferentially reaggregate with cells from the same tissue when they are mixed together. This tissue-specific recognition process in vertebrates is mainly mediated by a family of cell adhesion molecules because of their specific binding properties. Recent studies have revealed that two families of adhesion molecules, nectins and cadherins, are associated with each other, and these associations provide cells with the differential adhesive affinities required for cellular recognition and complex cellular pattern formations during development...
April 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28392351/tug1-promotes-lens-epithelial-cell-apoptosis-by-regulating-mir-421-caspase-3-axis-in-age-related-cataract
#16
Guoxing Li, Huiyang Song, Weihua Yang, Shanshan Zhang, Kaihui Nan, Peirong Lu
BACKGROUND: Age-related cataract is among the most common chronic disorders of ageing and the apoptosis of lens epithelial cells contributes to non-congenital cataract development. We amid to explore the role of TUG1 and miR-421 in the age-related cataract. METHODS: The expression level of TUG1, miR-421 and caspase-3 were detected by RT-qPCR. The apoptotic-related protein, caspase-3, Bax and blc-2 were analyzed by western blot. We performed ultraviolet (UV) irradiation to induce SAR01/04l cell apoptosis which was analyzed by flow cytometry...
April 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28390677/calibrated-flux-measurements-reveal-a-nanostructure-stimulated-transcytotic-pathway
#17
Tarianna Stewart, William T Koval, Samuel A Molina, Suzanne M Bock, James W Lillard, Russell F Ross, Tejal A Desai, Michael Koval
Transport of therapeutic agents across epithelial barriers is an important element in drug delivery. Transepithelial flux is widely used as a measure of transit across an epithelium, however it is most typically employed as a relative as opposed to absolute measure of molecular movement. Here, we have used the calcium switch approach to measure the maximum rate of paracellular flux through unencumbered intercellular junctions as a method to calibrate the flux rates for a series of tracers ranging in 0.6-900kDa in size across barriers composed of human colon epithelial (Caco-2) cells...
April 5, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28390676/nitric-oxide-donor-augments-antineoplastic-effects-of-arginine-deprivation-in-human-melanoma-cells
#18
Oksana Mayevska, Oleh Chen, Olena Karatsai, Yaroslav Bobak, Maryna Barska, Liliana Lyniv, Iuliia Pavlyk, Yuriy Rzhepetskyy, Natalia Igumentseva, Maria Jolanta Redowicz, Oleh Stasyk
Anticancer therapy based on recombinant arginine-degrading enzymes has been proposed for the treatment of several types of malignant cells deficient in arginine biosynthesis. One of the predicted side effects of such therapy is restricted bioavailability of nitric oxide as arginine catabolic product. Prolonged NO limitation may lead to unwanted disturbances in NO-dependent vasodilation, cardiovascular and immune systems. This problem can be overcome by co-supplementation with exogenous NO donor. However, NO may potentially counteract anticancer effects of therapy based on arginine deprivation...
April 5, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28389210/shape-of-my-heart-cell-cell-adhesion-and-cytoskeletal-dynamics-during-drosophila-cardiac-morphogenesis
#19
REVIEW
Christopher M J McFaul, Rodrigo Fernandez-Gonzalez
The fruit fly Drosophila melanogaster has recently emerged as an excellent system to investigate the genetics of cardiovascular development and disease. Drosophila provides an inexpensive and genetically-tractable in vivo system with a large number of conserved features. In addition, the Drosophila embryo is transparent, and thus amenable to time-lapse fluorescence microscopy, as well as biophysical and pharmacological manipulations. One of the conserved aspects of heart development from Drosophila to humans is the initial assembly of a tube...
April 4, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28389017/corrigendum-to-pna-combo-fish-from-combinatorial-probe-design-in-silico-to-vitality-compatible-specific-labelling-of-gene-targets-in-cell-nuclei-exp-cell-res-345-2016-51-59
#20
Patrick Müller, Jens Rößler, Jutta Schwarz-Finsterle, Eberhard Schmitt, Michael Hausmann
No abstract text is available yet for this article.
April 4, 2017: Experimental Cell Research
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