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Experimental Cell Research

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https://www.readbyqxmd.com/read/29913155/antifibrotic-role-of-pgc-1%C3%AE-sirna-against-tgf-%C3%AE-1-induced-renal-interstitial-fibrosis
#1
Jue-Long Wang, Chin-Wang Chen, Mu-Rou Tsai, Shu-Fen Liu, Tsuing-Jeu Hung, Yu-Ju-Hung, Wen-Teng Chang, Ming-Der Shi, Pei-Fang Hsieh, Yu-Lin Yang
Peroxisome proliferator-activated receptor coactivator-1 alpha (PGC-1α) is a transcriptional coactivator that regulates energy metabolism and mitochondrial biogenesis. Recently, mitochondrial dysfunction has been indicated as an established risk factor for the development of renal fibrosis. However, whether PGC-1α is involved in the pathogenesis of renal fibrosis is unknown. In this study, we treated NRK-49F (normal rat kidney fibroblast) cells with transforming growth factor-beta 1 (TGF-β1) for 24h to establish an in vitro fibrosis model...
June 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29913154/interactions-between-monomeric-cct%C3%AE-and-p150-glued-a-novel-function-for-cct%C3%AE-at-the-cell-periphery-distinct-from-the-protein-folding-activity-of-the-molecular-chaperone-cct
#2
Meriem Echbarthi, Josefine Vallin, Julie Grantham
Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone consisting of eight distinct protein subunits, that when oligomeric is essential for the folding of newly synthesized tubulin and actin. In addition to folding, CCT activity includes functions of individual subunits in their monomeric form. For example, when CCTδ monomer levels are increased in cultured mammalian cells, numerous cell surface protrusions are formed from retraction fibres, indicating that an underlying function for the CCTδ monomer exists...
June 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29913153/pag1-promotes-the-inherent-radioresistance-of-laryngeal-cancer-cells-via-activation-of-stat3
#3
Li Shen, Qing Ke, Jingjing Chai, Chuanyi Zhang, Li Qiu, Feng Peng, Xinzhou Deng, Zhiguo Luo
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1(PAG1) is a ubiquitous protein that is essential for the development and progression of various malignancies. A previous study in our laboratory confirmed that PAG1 plays an important role in modulating the inherent radioresistance of laryngeal cancer cells, but the underlying mechanisms are still poorly defined. In this study, we found that PAG1 was significantly increased in laryngeal cancer tissues compared to adjacent non-tumor tissues (P < 0...
June 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29908163/matrix-metalloproteinases-are-regulated-by-microrna-320-in-macrophages-and-are-associated-with-aortic-dissection
#4
Mingfang Liao, Sili Zou, Yan Bao, Jie Jin, Junlin Yang, Yandong Liu, Mark Green, Futang Yang, Lefeng Qu
Aortic dissection (AD) is the circumferential or transversal tear of the aorta wall that allows blood to infiltrate the layers. MicroRNA (miR) analyses have demonstrated a correlation between miR-320 family and AD. The underlying mechanism is yet unclear. The matrix metalloproteinases (MMPs) are a group of proteolytic enzymes that could catalyze the degeneration of the extracellular matrix and the destruction of the vasculature. In this study, we investigated whether miR-320 presented a role in regulating the production of MMPs in aortic dissection...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29908162/isolation-and-characterization-of-endothelial-colony-forming-cells-from-mononuclear-cells-of-rat-bone-marrow
#5
Shukui Yu, Zhongxuan Li, Wei Zhang, Zhengde Du, Ke Liu, Denghua Yang, Shusheng Gong
Transplantation of bone marrow-derived endothelial progenitor cells (BM-EPCs) has been used as a therapeutic strategy for vascular repair. However, it remains controversial whether BM-EPCs exhibit clonal endothelial colony-forming cell (ECFC) capacity, a characteristic of true EPCs. The aim of this study was to isolate and explore the cellular properties of BM-ECFCs. We isolated BM-ECFCs from rat bone marrow with high purity via an optimized method. This approach involved the removal of selective colonies based on the conventional differential adhesive culture method used to isolate ECFCs from peripheral and umbilical cord blood...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29908161/autophagy-attenuates-compression-induced-apoptosis-of-human-nucleus-pulposus-cells-via-mek-erk-nrf1-atg7-signaling-pathways-during-intervertebral-disc-degeneration
#6
Shuai Li, Wenbin Hua, Kun Wang, Yong Gao, Songfeng Chen, Wei Liu, Yu Song, Xinghuo Wu, Ji Tu, Liang Kang, Kangcheng Zhao, Liming Xiong, Yukun Zhang, Cao Yang
Autophagy dysfunction has been observed in intervertebral disc degeneration (IVDD) cells, a main contributing factor to cell death, but the precise role of autophagy during IVDD is still controversial. This study aimed to investigate the role of autophagy involved in the pathogenesis of human IVDD and determine the signal transduction pathways responsible for compression-induced autophagy in human nucleus pulposus (NP) cells. Autophagy, suppressing the induction of apoptosis, was activated in NP cells exposed to compression...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29908160/pseudo-phosphorylation-at-at8-epitopes-regulates-the-tau-truncation-at-aspartate-421
#7
Lan Cao, Yan Liang, Yunsheng Liu, Yuxia Xu, Wenbin Wan, Cuiqing Zhu
Tau pathology in Alzheimer's disease (AD) includes hyperphosphorylation and truncation of tau. Phosphorylation at S422 is found to suppress truncation of tau at D421 that leading to the generation of ΔTau. However, the interrelation between hyperphosphorylation and generation of ΔTau in AD remains elusive. In current study, staurosporine (Stau) induced ΔTau generation by caspases in SH-SY5Y cells with tau overexpression was found to be accompanied by a dramatic dephosphorylation at S422 and the epitope of the diagnostic antibody AT8 (S199+S202+T205), but a moderate dephosphorylation of PHF1 (S396+S404) epitope...
June 13, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29902537/nucleolin-is-expressed-in-patient-derived-samples-and-glioblastoma-cells-enabling-improved-intracellular-drug-delivery-and-cytotoxicity
#8
Joana Balça-Silva, Anália do Carmo, Hermínio Tão, Olinda Rebelo, Marcos Barbosa, Vivaldo Moura-Neto, Ana Bela Sarmento-Ribeiro, Maria Celeste Lopes, João Nuno Moreira
One of the major challenges in Glioblastoma (GBM) therapy relates with the existence of glioma stem-like cells (GSCs), known to be chemo- and radio-resistant. GSCs and non-stem GBM cells have the ability to interchange, emphasizing the importance of identifying common molecular targets among those cell sub-populations. Nucleolin overexpression has been recently associated with breast cancer sub-populations with different stem-like phenotype. The goal of this work was to evaluate the potential of cell surface nucleolin as a target in GBM cells...
June 11, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29902536/aldolase-promotes-the-development-of-cardiac-hypertrophy-by-targeting-ampk-signaling
#9
Yapeng Li, Dianhong Zhang, Lingyao Kong, Huiting Shi, Xinyu Tian, Lu Gao, Yuzhou Liu, Leiming Wu, Binbin Du, Zhen Huang, Cui Liang, Zheng Wang, Rui Yao, Yanzhou Zhang
Metabolic dysfunction is a hallmark of cardiac hypertrophy and heart failure. During cardiac failure, the metabolism of cardiomyocyte switches from fatty acid oxidation to glycolysis. However, the roles of key metabolic enzymes in cardiac hypertrophy are not understood fully. Here in the present work, we identified Aldolase A (AldoA) as a core regulator of cardiac hypertrophy. The mRNA and protein levels of AldoA were significantly up-regulated in transverse aortic constriction (TAC)- and isoproterenol (ISO)-induced hypertrophic mouse hearts...
June 11, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29902535/adam17-egfr-dependent-erk-activation-mediates-thrombin-induced-ctgf-expression-in-human-lung-fibroblasts
#10
Hui-Yu Chen, Chien-Huang Lin, Bing-Chang Chen
Lung fibroblasts play critical roles in fibrotic procedures and contribute to lung fibrosis. Several studies indicated that thrombin, a disintegrin and metalloproteinase 17 (ADAM17), and connective tissue growth factor (CTGF) participate in the formation of pulmonary fibrosis. In this study, we examined the involvement of the ADAM17/epidermal growth factor receptor (EGFR)/extracellular signal-regulated kinase (ERK) pathway in thrombin-stimulated CTGF manifestation in human lung fibroblasts (WI-38). We found that cells treated with thrombin significantly increased ADAM17 expression, ADAM17 and c-Jun phosphorylation in time-dependent manners...
June 11, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29894707/silencing-pfkp-inhibits-starvation-induced-autophagy-glycolysis-and-epithelial-mesenchymal-transition-in-oral-squamous-cell-carcinoma
#11
Guanhui Chen, Haichao Liu, Yadong Zhang, Jianfeng Liang, Yue Zhu, Ming Zhang, Dongsheng Yu, Cheng Wang, Jinsong Hou
The tumor starvation microenvironment plays a pivotal role in the malignant progression of cancer, which is closely related to autophagy, glycolysis, and epithelial mesenchymal transition (EMT). Nevertheless, the underlying mechanisms of the starvation-mediated malignant phenotype are still not well documented. In this study, we aimed to investigate the effect of starvation on glycolysis, autophagy, and EMT in OSCC and to further elucidate the key metabolic modulator. The results showed that starvation can induce autophagy, EMT, and enhanced glycolysis in OSCC cells...
June 9, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29894706/cd44-collaborates-with-erbb2-mediate-radiation-resistance-via-p38-phosphorylation-and-dna-homologous-recombination-pathway-in-prostate-cancer
#12
Ji-Wei Ma, Xiao Wang, Lei Chang, Xue-Yun Zhong, Haiyan Jing, Xiaolong Zhu, Shaoxiang Wang, WeiWei Xiao
CD44, a glycoprotein, has been reported to have relationship with resistance to radiation in prostate cancer (Cap) cells. However, its molecular mechanism remains unknown. In this study, we demonstrated that inhibited CD44 enhanced the radiosentivity in Cap cells. It has been hypothesized that CD44 combine with ERBB2 and activate downstream phosphated protein to mediate DNA damage repair. Therefore, we conducted a detailed analysis of effects of radiation by clonogenic assay and immunofluorescence stain for p-H2AX foci...
June 9, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29883714/exosomes-derived-from-endothelial-progenitor-cells-ameliorate-acute-lung-injury-by-transferring-mir-126
#13
Xu Wu, Zilong Liu, Lijuan Hu, Wenyu Gu, Lei Zhu
Endothelial progenitor cell (EPC) has potential to attenuate pulmonary inflammation and injury. As a pivotal paracrine entity of stem cells, whether EPC-derived exosomes (EPC-Exos) contribute to acute lung injury (ALI) remains unknown. Exosomes were purified from conditional medium of EPCs, and then characterized by electron micrograph and immunoblotting. A model of ALI was induced by lipopolysaccharide (LPS) and then rats were transplanted with EPC-Exos. The underlying mechanisms of action of EPC-Exos were examined in vitro endothelial functional assays including the TEER, proliferation (CKK-8), angiogenesis and migration...
June 5, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29883713/inhibition-of-mir-135b-by-sp-1-promotes-hypoxia-induced-vascular-endothelial-cell-injury-via-hif-1%C3%AE
#14
Songbai Yang, Jian Yin, Xuhui Hou
Myocardial hypoxia-induced endothelial cell apoptosis contributes to cardiac dysfunction, such as myocardial infarction (MI), myocardial ischemia, and heart failure. Thus, it is important to investigate the molecular mechanisms of vascular endothelial cells (VECs) during exposure to hypoxia. SP-1 is an important regulator of cytokines associated with cell functions. We found that SP-1 expression increased in human umbilical vein endothelial cells (HUVECs) exposed to hypoxia by western blot. Then the SP-1 siRNA was transfected into HUVECs under hypoxic condition...
June 5, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29883712/differentiation-in-stem-progenitor-cells-along-fetal-or-adult-hepatic-stages-requires-transcriptional-regulators-independently-of-oscillations-in-microrna-expression
#15
Sriram Bandi, Sanchit Gupta, Tatyana Tchaikovskaya, Sanjeev Gupta
Understanding mechanisms in lineage differentiation is critical for organ development, pathophysiology and oncogenesis. To determine whether microRNAs (miRNA) may serve as drivers or adjuncts in hepatic differentiation, we studied human embryonic stem cell-derived hepatocytes and primary hepatocytes representing fetal or adult stages. Model systems were used for hepatic lineage advancement or regression under culture conditions with molecular assays. Profiles of miRNA in primary fetal and adult hepatocytes shared similarities and distinctions from pluripotent stem cells or stem cell-derived early fetal-like hepatocytes...
June 5, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29883711/microrna-21a-5p-promotes-fibrosis-in-spinal-fibroblasts-after-mechanical-trauma
#16
Wenzhao Wang, Shi Tang, Hongfei Li, Ronghan Liu, Yanlin Su, Lin Shen, Mingjie Sun, Bin Ning
Traumatic spinal cord injury (SCI) causes permanent disability to at least 180,000 people per year worldwide. Early regulation of spinal fibroblast proliferation may inhibit fibrotic scar formation, allowing the creation of a favorable environment for neuronal regeneration and thereby enhancing recovery from traumatic SCIs. In this study, we aimed to identify the role of microRNA-21-5p (miR-21a-5p) in regulating spinal fibroblasts after mechanical trauma and to investigate the dysregulation of miR-21a-5p in the pathological process of spinal SCI...
June 5, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29864401/cs2164-exerts-an-antitumor-effect-against-human-non-hodgkin-s-lymphomas-in-vitro-and-in-vivo
#17
Manman Deng, Yuanfei Shi, Kai Chen, Haijun Zhao, Yan Wang, Siting Xie, Jintao Zhao, Yiming Luo, Zhihong Fang, Yaqun Fan, Bing Xu
Non-Hodgkin's lymphomas (NHLs) are a heterogeneous group of lymphoproliferative disorders. Mounting studies have suggested an involvement of angiogenesis signaling in NHLs progression and resistance to treatment. In this study, we investigated the cytotoxicity of CS2164, a novel receptor tyrosine kinase inhibitor selectively targeting VEGFR-2 and Aurora B in NHL cells. By in vitro culture system and in vivo xenograft model, we found that CS2164 significantly inhibited cell growth and abolished clonogenicity in NHL cells in a dose- and time-dependent manner...
June 1, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29864400/assessment-of-the-hepatocytic-differentiation-ability-of-human-skin-derived-abcb5-stem-cells
#18
Lysann Tietze, Sandra Winkler, Madlen Hempel, Mark Andreas Kluth, Nils Tappenbeck, Christoph Ganss, Steven Dooley, Bruno Christ
The continuously decreasing willingness for liver donation aggravates treatment of end-stage liver diseases requiring organ transplantation as the only curative strategy. Cell therapy approaches using human hepatocytes or stem cell-derived hepatocyte-like cells may be a therapeutic option out of this dilemma. ABCB5-positive mesenchymal stromal cells from human skin featured promising potential to treat immune-mediated diseases. Since most of chronic liver diseases involve exaggerating immune mechanisms, it was the aim to demonstrate in this study, whether ABCB5+ stem cells may serve as a resource to generate hepatocytic cells for application in liver cell transplantation...
June 1, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29859752/lncrba-gsa5-up-regulated-by-ox-ldl-aggravates-inflammatory-response-and-mmp-expression-in-thp-1-macrophages-by-acting-like-a-sponge-for-mir-221
#19
Jiumin Ye, Chongbao Wang, Dongqi Wang, Huijun Yuan
Atherosclerosis is a chronic inflammatory process, plaque rupture and subsequent thrombosis underline the major causes of acute cardio-cerebral vascular diseases. Long non-coding RNAs (lncRNAs) participate in diverse pathologic processes, including inflammation and myocardial infarction. Recent study confirmed the elevation of lncRNA growth arrest-specific 5 (GAS5) in atherosclerotic rats. In this study, we aimed to explore the role and mechanism of GAS5 in the progression of atherosclerotic plaque. Here, expression of GAS5 was enriched in atherosclerotic plaques and THP-1 macrophage exposed to oxidized low-density lipoprotein (ox-LDL)...
May 31, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29859140/involvement-of-lpa-signaling-via-lpa-receptor-2-in-the-promotion-of-malignant-properties-in-osteosarcoma-cells
#20
Kaede Takahashi, Kaori Fukushima, Kosuke Tanaka, Kanako Minami, Kaichi Ishimoto, Shiho Otagaki, Nobuyuki Fukushima, Kanya Honoki, Toshifumi Tsujiuchi
Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors mediates various biological effects in cancer cells. This study aimed to investigate the roles of LPA receptors in the regulation of cellular functions during tumor progression in osteosarcoma cells. Long-term cisplatin (CDDP)-treated MG63-C and MG63-R7-C cells were generated from osteosarcoma MG-63 and highly-migratory MG63-R7 cells, respectively. LPAR2 and LPAR3 expression levels were significantly higher in MG63-C cells than in MG-63 cells, while LPAR1 expression was reduced...
May 30, 2018: Experimental Cell Research
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