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Experimental Cell Research

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https://www.readbyqxmd.com/read/29042209/chondrocytes-damage-induced-by-t-2-toxin-via-wnt-%C3%AE-catenin-signaling-pathway-is-involved-in-the-pathogenesis-of-an-endemic-osteochondropathy-kashin-beck-disease
#1
Xi Wang, Yujie Ning, Pan Zhang, Lei Yang, Yingting Wang, Xiong Guo
Kashin-Beck disease (KBD), an endemic osteochondropathy, is characterized by cartilage degeneration which is caused by abnormal catabolism in the extracellular matrix (ECM). In this study, we investigated the expression of the Wnt/β-catenin signaling pathway in KBD pathogenesis. Among the proteins involved in the Wnt/β-catenin signaling pathway, WNT-3A, FZD1, SOX9, and β-catenin were up-regulated, while FRZB was down-regulated in KBD cartilage. C28/I2 cells were evaluated for cell viability using the MTT assay after exposure to T-2 toxin, a suspicious environmental pathogenic factors of KBD...
October 14, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29031557/regulation-of-hc11-mouse-breast-epithelial-cell-differentiation-by-the-e-cadherin-rac-axis
#2
Maximilian Niit, Rozanne Arulanandam, Jamaica Cass, Mulu Geletu, Victoria Hoskin, Graham Côté, Patrick Gunning, Bruce Elliott, Leda Raptis
It was previously demonstrated that differentiation of some established breast epithelial cell lines requires confluence and stimulation with hydrocortisone, insulin and prolactin inducers. We and others previously demonstrated that E-cadherin engagement, which is favored under conditions of confluence, increases the levels and activity of the Rac small GTPase. To investigate the functional relationship between the transforming ability of Rac and its role as an integral component of the differentiative E-cadherin signaling pathway, we introduced a mutationally activated form of Rac, Rac(V12), into the mouse breast epithelium-derived cell line, HC11...
October 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29031634/fecal-bacteria-from-treatment-naive-crohn-s-disease-patients-can-skew-helper-t-cell-responses
#3
Fei Ma, Yi Zhang, Junjie Xing, Xiaoling Song, Ling Huang, Hao Weng, Xiangsong Wu, Emma Walker, Zhongchuan Wang
Many studies have demonstrated that the inflamed mucosa of Crohn's disease (CD) patients presented a disturbed gut commensal community, and the shift in microbial composition and species variety is associated with disease severity. To establish a link between changes in the intestinal bacterial composition and the alteration of inflammation, we obtained fecal bacteria from CD patients and non-CD controls. The bacteria were then used to stimulate the peripheral blood mononuclear cells (PBMCs) from one non-CD individual...
October 11, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29031633/translation-of-noncoding-rnas-focus-on-lncrnas-pri-mirnas-and-circrnas
#4
REVIEW
Lian-Ju Li, Rui-Xue Leng, Yin-Guang Fan, Hai-Feng Pan, Dong-Qing Ye
Mammalian genome is pervasively transcribed, producing large number of noncoding RNAs (ncRNAs), including long noncoding RNAs (lncRNAs), primary miRNAs (pri-miRNA), and circular RNAs (circRNAs). The translation of these ncRNAs has long been overlooked. Increasing studies, however, based on ribosome profiling in various organisms provide important clues to unanticipated translation potential of lncRNAs. Moreover, a few functional peptides encoded by lncRNAs and pri-miRNAs underline the significance of their translation...
October 11, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29024700/high-ybx1-expression-indicates-poor-prognosis-and-promotes-cell-migration-and-invasion-in-nasopharyngeal-carcinoma
#5
Lei-Lei Zhou, Jie Ni, Wan-Ting Feng, Rong Yao, Shun Yue, Ya-Ning Zhu, Hai-Yan Tang, Ling-Yun Lv, Ji-Feng Feng, Wei-Guo Zhu
Y-box binding protein-1 (YBX1) is a multifunctional protein and often acts as an indicator of poor prognosis in cancers. Increasing evidence has shown that the levels of YBX1 protein were closely associated with multidrug resistance, relapse, metastasis and poor prognosis in cancers. However, its role in nasopharyngeal carcinoma (NPC) metastasis remains unknown. In our study, we discovered that the expression of YBX1 was increased in nasopharyngeal carcinoma tissues. YBX1 protein levels positively correlated with T stage and metastasis of NPC patients...
October 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29017757/effects-of-retinoic-acid-signaling-on-extraocular-muscle-myogenic-precursor-cells-in-vitro
#6
Sadie L Hebert, Krysta R Fitzpatrick, Samantha A McConnell, Anja Cucak, Ching Yuan, Linda K McLoon
One major difference between limb and extraocular muscles (EOM) is the presence of an enriched population of Pitx2-positive myogenic precursor cells in EOM compared to limb muscle. We hypothesize that retinoic acid regulates Pitx2 expression in EOM myogenic precursor cells and that its effects would differ in leg muscle. The two muscle groups expressed differential retinoic acid receptor (RAR) and retinoid X receptor (RXR) levels. RXR co-localized with the Pitx2-positive cells but not with those expressing Pax7...
October 7, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29017756/biomechanical-strain-induced-modulation-of-proliferation-coincides-with-an-erk1-2-independent-nuclear-yap-localization
#7
Diana Huelter-Hassler, Martin Wein, Simon D Schulz, Susanne Proksch, Thorsten Steinberg, Britta A Jung, Pascal Tomakidi
Biomechanical strain induces activation of the transcriptional co-activator yes-associated protein (YAP) by nuclear re-distribution. Recent findings indicate that the mechanically responsive mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) 1/2 is involved in the amount of nuclear YAP, reflecting its activation. In this context, we conducted experiments to detect how biomechanical strain acts on the subcellular localization of YAP in periodontal cells. To this end, cells were subjected to 2...
October 7, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28988741/smad7-alleviates-glomerular-mesangial-cell-proliferation-via-the-ros-nf-%C3%AE%C2%BAb-pathway
#8
Nana Lin, Zequan Ji
OBJECTIVE: The aim of this study was to demonstrate that altered gene expression of Smad7regulated NF-κB expression and ROS production on Ang II (Angiotensin II)-induced rat glomerular mesangial cell (GMC) proliferation. METHODS: pAdTrack-CMV-Smad7 was transduced into rat GMC by adeno-transduction using an ADV (adenovirus)-mediated vector in vivo. Diphenylene iodonium chloride (DPI) pre-treated GMC, and blocked ROS generation as determined by DCFH-DA method. Altered expressions of IκBα and p65 were monitored by Western blot analysis and immunofluorescence...
October 5, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28987541/robo-signaling-regulates-the-production-of-cranial-neural-crest-cells
#9
Yan Li, Xiao-Tan Zhang, Xiao-Yu Wang, Guang Wang, Manli Chuai, Andrea Münsterberg, Xuesong Yang
Slit/Robo signaling plays an important role in the guidance of developing neurons in developing embryos. However, it remains obscure whether and how Slit/Robo signaling is involved in the production of cranial neural crest cells. In this study, we examined Robo1 deficient mice to reveal developmental defects of mouse cranial frontal and parietal bones, which are derivatives of cranial neural crest cells. Therefore, we determined the production of HNK1(+) cranial neural crest cells in early chick embryo development after knock-down (KD) of Robo1 expression...
October 5, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28987542/establishment-of-twist-1-and-tgfbr2-as-direct-targets-of-microrna-20a-in-mesenchymal-to-epithelial-transition-of-breast-cancer-cell-line-mda-mb-231
#10
Soumasree De, Sayantani Das, Srimoyee Mukherjee, Sainy Das, Sumita Sengupta Bandyopadhyay
Messenchymal to epithelial transition (MET) is a significant physiological phenomenon involved in embryogenesis and cancer. This study aims at investigating the mechanism of microRNA-20a (miR-20a) mediated regulation of mesenchymal to epithelial transition and identification of its direct target genes in breast cancer cell-line, MDA-MB-231. Reduced migratory and invasive property, altered cellular morphology along with reduced capability for attachment to basement membrane was acquired by over-expression of miR-20a in invasive MDA-MB-231 cell-line initially expressing low level of this micro-RNA, indicating direct correlation between abundance of miR-20a and metastatic property...
October 4, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28987540/serelaxin-inhibits-differentiation-and-fibrotic-behaviors-of-cardiac-fibroblasts-by-suppressing-alk-5-smad2-3-signaling-pathway
#11
Xue-Ping Wu, Hai-Jie Wang, Yong-Li Wang, Hao-Ran Shen, Yu-Zhen Tan
Serelaxin, a recombinant form of human relaxin-2, is currently regarded as a novel drug for treatment of acute heart failure. However, whether therapeutic effects of serelaxin are achieved by inhibiting cardiac fibrosis remains unclear. In this study, we investigate effects of serelaxin on inhibiting cardiac fibrosis. Cardiac fibroblasts (CFs) were isolated from the hearts of adult rats. Effects of serelaxin on differentiation of CFs towards myofibroblasts (MFs) and their fibrotic behaviors after inducion with TGF-β1 were examined...
October 4, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28986067/counterintuitive-regulatory-t-cell-responses-are-observed-in-senior-patients-with-community-acquired-pneumonia-and-are-related-to-disease-severity
#12
Lu-Qin Bian, Ying Bi, Shao-Wei Zhou, Zi-Dan Chen, Jun Wen, Jin Shi, Ling Mao, Ling Wang
Senior individuals older than 65 years of age are at a disproportionally higher risk of developing pneumonia. Impaired capacity to defend against airway infections may be one of the reasons. It is generally believed that weaker regulatory T cell responses may be beneficial to host defense against pathogens. In senior patients with community-acquired bacterial pneumonia, we investigated the frequencies and functions of regulatory T cells. Interestingly, we found that compared to age- and sex-matched healthy controls, senior pneumonia patients presented lower frequencies of Foxp3-expressing and Helios-expressing CD4(+) T cells...
October 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28986066/the-crosstalk-between-sirt1-and-keap1-nrf2-are-anti-oxidative-pathway-forms-a-positive-feedback-loop-to-inhibit-fn-and-tgf-%C3%AE-1-expressions-in-rat-glomerular-mesangial-cells
#13
Kaipeng Huang, Xiang Gao, Wentao Wei
Oxidative stress aroused by advanced glycation-end products (AGEs) is a culprit in the pathological progression of diabetic nephropathy. Both Sirt1 and the Keap1/Nrf2/ARE anti-oxidative pathway exert crucial inhibitory effects on the development of diabetic nephropathy. Our previous study has confirmed that Sirt1 activation can inhibit the upregulation of fibronectin (FN) and transforming growth factor-β1 (TGF-β1) by promoting Keap1/Nrf2/ARE pathway in glomerular mesangial cells (GMCs) challenged with AGEs...
October 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28982539/trepnema-pallidum-flagellins-stimulate-mmp-9-and-mmp-13-expression-via-tlr5-and-mapk-nf-%C3%AE%C2%BAb-signaling-pathways-in-human-epidermal-keratinocytes
#14
Chuanhao Jiang, Man Xu, Xingxing Kuang, Jinhong Xiao, Manyi Tan, Yafeng Xie, Yongjian Xiao, Feijun Zhao, Yimou Wu
Syphilis is a chronic disease caused by Treponema pallidum and the pathogenesis is still unclear. T. pallidum infection induced inflammatory responses are involved in the immunopathological damage in skin and other tissues. Flagellin, the monomeric subunit of bacterial flagella, is a classic pathogen associated molecular patterns (PAMPs) that interacts to TLR5 and induces inflammatory responses. Keratinocytes, as immune sentinels recognize the PAMPs via TLRs, play an important role in skin innate immune response...
October 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28965867/cxcr5-cd8-t-cells-potently-infiltrate-pancreatic-tumors-and-present-high-functionality
#15
Minghui Bai, Youwei Zheng, Haichao Liu, Baowei Su, Yong Zhan, Hua He
Despite continued improvement in conventional therapy, pancreatic cancer continues to be one of the deadliest tumors worldwide with abysmal 5-year survival rate. New immunotherapeutic strategies that aim at improving antitumor cytotoxic CD8(+) T cell responses are being developed in solid tumors. To assist the development of immunotherapies, we investigated the CD8(+) T cells in pancreatic cancer patients. Compared to healthy individuals, pancreatic cancer patients presented a significant enrichment in the frequency of CD8(+)CXCR5(+) T cells...
September 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28965866/the-role-of-c1qbp-in-csf-1-dependent-pkc%C3%AE-activation-and-macrophage-migration
#16
Yong Wang, Jing Su, Bo Yuan, Donghe Fu, Yuanjie Niu, Dan Yue
Macrophages view as double agents in tumor progression. Trafficking of macrophages to the proximity of tumors is mediated by colony-stimulating factor-1 (CSF-1), a growth factor. In this study, we investigated the role of complement1q-binding protein (C1QBP)/ atypical protein kinase C ζ (PKCζ) in CSF-1-induced macrophage migration. Disruption of C1QBP expression impaired chemotaxis and adhesion of macrophage. Phosphorylation of PKCζ is an essential component in macrophage chemotaxis signaling pathway. C1QBP could interact with PKCζ in macrophage...
September 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28964779/the-long-noncoding-rna-casc2-inhibits-tumorigenesis-through-modulating-the-expression-of-pten-by-targeting-mir-18a-5p-in-esophageal-carcinoma
#17
Wenjing Zhang, Wei He, Jianfeng Gao, Yuanyuan Wang, Wenqiao Zang, Ziming Dong, Guoqiang Zhao
Abundant evidence indicates that long noncoding RNAs (lncRNAs) play an important role in various cellular processes, tumorigenesis, and the development of cancer. An increasing number studies are exploring this lncRNA-mediated mechanism. CASC2 is a novel gene in lncRNAs, located at chromosome 10q26. The present study aimed to examine the expression of lncRNA CASC2 in esophageal carcinoma (EC) tissue and to explore the effect of the upregulation of CASC2 on EC cell lines and specific regulatory mechanisms. We found the expression of CASC2 to be significantly downregulated in EC tissues and EC cell lines; moreover, low expression of CACS2 was associated with advanced TNM stage and lymph node metastases...
September 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28964780/gastric-cancer-tissue-derived-mesenchymal-stem-cells-impact-peripheral-blood-mononuclear-cells-via-disruption-of-treg-th17-balance-to-promote-gastric-cancer-progression
#18
Mei Wang, Bin Chen, Xiao-Xian Sun, Xiang-Dong Zhao, Yuan-Yuan Zhao, Li Sun, Chang-Gen Xu, Bo Shen, Zhao-Liang Su, Wen-Rong Xu, Wei Zhu
Gastric cancer tissue-derived mesenchymal stem cells (GC-MSCs) are important resident stromal cells in the tumor microenvironment (TME) and have been shown to play a key role in gastric cancer progression. Whether GC-MSCs exert a tumor-promoting function by affecting anti-tumor immunity is still unclear. In this study, we used GC-MSC conditioned medium (GC-MSC-CM) to pretreat peripheral blood mononuclear cells (PBMCs) from healthy donors. We found that GC-MSC-CM pretreatment markedly reversed the inhibitory effect of PBMCs on gastric cancer growth in vivo, but did not affect functions of PBMCs on gastric cancer cell proliferation, cell cycle and apoptosis in vitro...
September 27, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28962916/igf-i-regulates-ht1080-fibrosarcoma-cell-migration-through-a-syndecan-2-erk-ezrin-signaling-axis
#19
Maria Mytilinaiou, Dragana Nikitovic, Aikaterini Berdiaki, Antonis Papoutsidakis, Dionysios J Papachristou, Aristidis Tsatsakis, George N Tzanakakis
Fibrosarcoma is a tumor of mesenchymal origin, originating from fibroblasts. IGF-I is an anabolic growth factor which exhibits significant involvement in cancer progression. In this study, we investigated the possible participation of syndecan-2 (SDC-2), a cell membrane heparan sulfate (HS) proteoglycan on IGF-I dependent fibrosarcoma cell motility. Our results demonstrate that SDC-2-deficient HT1080 cells exhibit attenuated IGF-I-dependent chemotactic migration (p<0.001). SDC-2 was found to co-localize to IGF-I receptor (IGF-IR) in a manner dependent on IGF-I activity (P ≤ 0...
September 26, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28958711/glycosylation-status-of-bone-sialoprotein-and-its-role-in-mineralization
#20
Lan Xu, Zhenqing Zhang, Xue Sun, Jingjing Wang, Wei Xu, Lv Shi, Jiaojiao Lu, Juan Tang, Jingjing Liu, Xiong Su
The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. However, the structural details of its different glycosylation linkages have not been well studied and their functions in bone homeostasis are not clear. Previous studies suggested that the O-glycans, but not the N-glycans on BSP, are highly sialylated. Herein, we employed tandem mass spectrometry (MS/MS) to demonstrate that the N-glycanson the recombinant human integrin binding sialoprotein (rhiBSP) are also enriched in sialic acids (SAs) at their termini...
September 25, 2017: Experimental Cell Research
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