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Experimental Cell Research

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https://www.readbyqxmd.com/read/29233684/mmu-mir-1963-negatively-regulates-the-ameloblast-differentiation-of-ls8-cell-line-by-directly-targeting-smoc2-3-utr
#1
Yue Wang, Huaiguang Chang, Haochen Liu, Yang Liu, Dong Han, Jinhao Xing, Hongshan Zhao, Hailan Feng
RUNX2 is a key regulator of osteogenic differentiation and odontoblastic differentiation. RUNX2 mutations could cause Cleidocranial dysplasia (CCD; OMIM119600), which is featured by abnormal development of bone and teeth. By using microRNA array, we identified a large number of microRNAs that showed different expression between wild-type Runx2 group and mutant groups. The aim of this study is to find out the effect of mmu-miR-1963, which was downregulated in all mutant Runx2 groups, on the ameloblast differentiation of LS8 cells...
December 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29233683/caspase-mediated-cleavage-of-x-ray-repair-cross-complementing-group-4-promotes-apoptosis-by-enhancing-nuclear-translocation-of-caspase-activated-dnase
#2
Yumi Sunatani, Radhika Pankaj Kamdar, Mukesh Kumar Sharma, Tadashi Matsui, Ryo Sakasai, Mitsumasa Hashimoto, Yasuhito Ishigaki, Yoshihisa Matsumoto, Kuniyoshi Iwabuchi
X-ray repair cross-complementing group 4 (XRCC4), a repair protein for DNA double-strand breaks, is cleaved by caspases during apoptosis. In this study, we examined the role of XRCC4 in apoptosis. Cell lines, derived from XRCC4-deficient M10 mouse lymphoma cells and stably expressing wild-type XRCC4 or caspase-resistant XRCC4, were established and treated with staurosporine (STS) to induce apoptosis. In STS-induced apoptosis, expression of wild-type, but not caspase-resistant, XRCC4 in XRCC4-deficient cells enhanced oligonucleosomal DNA fragmentation and the appearance of TUNEL-positive cells by promoting nuclear translocation of caspase-activated DNase (CAD), a major nuclease for oligonucleosomal DNA fragmentation...
December 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29233682/potential-role-of-mg53-in-the-regulation-of-transforming-growth-factor-%C3%AE-1-induced-atrial-fibrosis-and-vulnerability-to-atrial-fibrillation
#3
Jingwen Guo, Fengpeng Jia, Yingjiu Jiang, Qiang Li, Yucheng Yang, Minghan Xiao, Hua Xiao
Atrial fibrosis plays a critical role in atrial fibrillation (AF) by the transforming growth factor (TGF)-β1/Smad pathway. The disordered differentiation, proliferation, migration and collagen deposition of atrial fibroblasts play significant roles in atrial fibrosis. Mitsugumin (MG)53 is predominantly expressed in myocardium of rodents and has multiple biological functions. However, the role of MG53 in cardiac fibrosis remains unclear. This study provided clinical and experimental evidence for the involvement of MG53 in atrial fibrosis in humans and atrial fibrosis phenotype in cultured rat atrial fibroblasts...
December 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29229447/hnrnp-a1-promotes-keratinocyte-cell-survival-post-uvb-radiation-through-pi3k-akt-mtor-pathway
#4
Jianguo Feng, Yi Liao, Xichao Xu, Qian Yi, Ling He, Liling Tang
hnRNP A1 acts as a critical splicing factor in regulating many alternative splicing events in various physiological and pathophysiological progressions. hnRNP A1 is capable of regulating UVB-induced hdm2 gene alternative splicing according to our previous study. However, the biological function and underlying molecular mechanism of hnRNP A1 in cell survival and cell cycle in response to UVB irradiation are still unclear. In this study, silencing hnRNP A1 expression by siRNA transfection led to decreased cell survival after UVB treatment, while promoting hnRNP A1 by lentiviruse vector resulted in increased cell survival...
December 8, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29225051/%C3%AE-taxilin-temporally-regulates-centrosome-disjunction-in-a-nek2a-dependent-manner
#5
Tomohiko Makiyama, Satoru Higashi, Hiroshi Sakane, Satoru Nogami, Hiromichi Shirataki
Never in mitosis A-related kinase 2A (Nek2A), a centrosomal serine/threonine kinase, is involved in mitotic progression by regulating the centrosome cycle. Particularly, Nek2A is necessary for dissolution of the intercentriole linkage between the duplicated centrosomes prior to mitosis. Nek2A activity roughly parallels its cell cycle-dependent expression levels, but the precise mechanism regulating its activity remains unclear. In this study, we found that γ-taxilin co-localized with Nek2A at the centrosome during interphase and interacted with Nek2A in yeast two-hybrid and pull-down assays and that γ-taxilin regulated centrosome disjunction in a Nek2A-dependent manner...
December 7, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29225050/inhibition-of-the-mitochondrial-calcium-uniporter-prevents-il-13-and-allergen-mediated-airway-epithelial-apoptosis-and-loss-of-barrier-function
#6
Sara C Sebag, Olha M Koval, John D Paschke, Christopher J Winters, Alejandro P Comellas, Isabella M Grumbach
Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca2+ uniporter (MCU) resides in the inner mitochondrial membrane and controls mitochondrial Ca2+ uptake into the mitochondrial matrix. To understand the function of MCU in models of allergic asthma, in vitro and in vivo studies were performed using models of functional deficiency or knockout of MCU...
December 7, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29223442/exosomes-derived-from-imatinib-resistant-chronic-myeloid-leukemia-cells-mediate-a-horizontal-transfer-of-drug-resistant-trait-by-delivering-mir-365
#7
Qing-Hua Min, Xiao-Zhong Wang, Jing Zhang, Qing-Gen Chen, Shu-Qi Li, Xiao-Qing Liu, Jing Li, Jing Liu, Wei-Ming Yang, Yu-Huan Jiang, Yan-Mei Xu, Jin Lin, Qiu-Fang Gao, Fan Sun, Lei Zhang, Bo Huang
Chronic myeloid leukemia (CML) is a malignant disorder of hematopoietic stem/progenitor cells. Majority of patients can be effectively treated with tyrosine kinase inhibitors (TKIs) such as imatinib, but a portion of patients will develop drug resistance. Accumulated evidences have identified exosomes in cancer as promoters of tumor progression. Herein, we found that exosomes derived from imatinib resistant CML cells can be internalized into sensitive CML cells and confer drug-resistance traits. We also demonstrated a significant higher level of miR-365 in exosomes derived from drug-resistant CML cells compared with those from sensitive ones using microarray and qRT-PCR...
December 6, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29208462/microrna-630-inhibits-breast-cancer-progression-by-directly-targeting-bmi1
#8
Xiu-Feng Gong, An-Lu Yu, Jun Tang, Chen-Long Wang, Jian-Rong He, Guo-Qiang Chen, Qian Zhao, Ming He, Ci-Xiang Zhou
MicroRNAs (miRNAs) play critical roles in breast cancer cell biological processes, including proliferation and apoptosis by inhibiting the expression of their target genes. Herein, we reported that miR-630 overexpression initiates apoptosis, blocks cell cycle progression and suppresses cell proliferation in breast cancer cells. Furthermore, BMI1, a member of polycomb group family, was identified as a direct target of miR-630, and there was a negative correlation between the expression levels of BMI1 and miR-630 in human breast cancer samples...
December 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29208461/simvastatin-inhibits-the-development-of-radioresistant-esophageal-cancer-cells-by-increasing-the-radiosensitivity-and-reversing-emt-process-via-the-pten-pi3k-akt-pathway
#9
Yingying Jin, Kun Xu, Qingjuan Chen, Baofeng Wang, Jiyuan Pan, Shan Huang, Yang Wei, Hongbing Ma
Acquired radioresistance compromises the efficacy of radiotherapy for carcinomas including esophageal cancer (EC), thus resulting in recurrence and poor survival. Recent research corroborated radiosensitive function of simvastatin in stem-like breast cancer cells. However, its role in EC radioresistance remains poorly elucidated. Here, we developed a radioresistant EC cell line Ec9706-R with higher resistance to irradiation relative to control Ec9706 cells. Intriguingly, Ec9706-R cells exhibited epithelial-mesenchymal transition (EMT) characteristics with high invasion and migration ability...
December 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29208460/ralgps2-is-involved-in-tunneling-nanotubes-formation-in-5637-bladder-cancer-cells
#10
A D'Aloia, G Berruti, B Costa, C Schiller, R Ambrosini, V Pastori, E Martegani, M Ceriani
RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor (GEF) for RalA containing a PH domain and an SH3-bindind region and it is involved in several cellular processes, such as cytokinesis, control of cell cycle progression, differentiation, cytoskeleton organization and rearrangement. Up to now, few data have been published regarding RalGPS2 role in cancer cells, and its involvement in bladder cancer is yet to be established. In this paper we demonstrated that RalGPS2 is expressed in urothelial carcinoma-derived 5637 cancer cells and is essential for cellular growth...
December 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29208459/mir-3202-protects-smokers-from-chronic-obstructive-pulmonary-disease-through-inhibiting-faim2-an-in-vivo-and-in-vitro-study
#11
Wen Shen, Jia Liu, Minjuan Fan, Shukun Wang, Yang Zhang, Linqiao Wen, Ruili Wang, Wei Wei, Na Li, You Zhang, Guohou Zhao
Previous study found the variable miR-3202 as a potential biomarker in smoker with or without chronic obstructive pulmonary disease (COPD). This study aims to identify the molecular involvement of miR-3202 in the pathophysiology of COPD. Level of miR-3202 in blood sample of non-smoker non-COPD(C), smoker without COPD(S), smoker with stable COPD(S-COPD) and smoker with acute exacerbation COPD(AE-COPD) was observed by quantitative real-time PCR. By bioinformatics prediction, Fas apoptotic inhibitory molecule 2 (FAIM2) was identified as a potential target of miR-3202...
December 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29208458/inhibition-of-tgf-%C3%AE-pathway-reverts-extracellular-matrix-remodeling-in-t-cruzi-infected-cardiac-spheroids
#12
Patrícia M Ferrão, Líndice M Nisimura, Otacílio C Moreira, Marcelo G Land, Mirian C Pereira, Leila de Mendonça-Lima, Tania C Araujo-Jorge, Mariana C Waghabi, Luciana R Garzoni
Chagasic cardiomyopathy (CC) is the main manifestation of Chagas Disease (CD). CC is a progressive dysfunctional illness, in which transforming growth factor beta (TGF-β) plays a central role in fibrogenesis and hypertrophy. In the present study, we tested in a three-dimensional (3D) model of cardiac cells culture (named cardiac spheroids), capable of mimicking the aspects of fibrosis and hypertrophy observed in CC, the role of TGF-β pathway inhibition in restoring extracellular matrix (ECM) balance disrupted by T...
December 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29197556/mir-195-inhibited-abnormal-activation-of-osteoblast-differentiation-in-mc3t3-e1-cells-via-targeting-raf-1
#13
Chen Chao, Feng Li, Zhiping Tan, Weizhi Zhang, Yifeng Yang, Cheng Luo
BACKGROUND: Recent reports have demonstrated that RAF-1L613V (a mutant of RAF-1) mutant mice show bone deformities similar to Noonan syndrome. It has been suggested that RAF-1L613V might abnormally activate osteoblast differentiation of MC3T3-E1 cells. METHODS: To demonstrate that RAF-1 is associated with bone deformity and that RAF-1L613V dependent bone deformity could be inhibited by microRNA-195 (miR-195), we first investigated the amplifying influence of wild-type RAF-1 (WT) or RAF-1L613V (L613V) on the viability and differentiation of MC3T3-E1 cells induced by bone morphogenetic protein-2 (BMP-2) via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis...
November 29, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196167/cathepsin-b-links-oxidative-stress-to-the-activation-of-nlrp3-inflammasome
#14
Hua Bai, Bo Yang, Wenfeng Yu, Yan Xiao, Dejun Yu, Qifang Zhang
Oxidative stress-mediated activation of NLRP3 inflammasome in microglia is critical in the development of neurodegerative diseases such as Alzheimer's disease (AD), Parkinson disease (PD). However, the mechanism underlying oxidative stress activates NLRP3 inflammasome remains exclusive. Here we demonstrated cathepsin B (CTSB) as a regulator of the activation of NLRP3 inflammasome by H2O2. H2O2 induced IL-1β secretion in NLRP3 inflammasome-dependent manner. H2O2 treatment increased CTSB activity, which in turn activated NLRP3 inflammasome, and subsequently processed pro-caspase-1 cleavage into caspase-1, resulting in IL-1 β secretion...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196166/1-25-oh-2d3-attenuates-pulmonary-arterial-hypertension-via-microrna-204-mediated-tgfbr2-smad-signaling
#15
Hua Yu, Min Xu, Yizhi Dong, Jingjing Liu, Yaozhe Li, Wei Mao, Jindan Wang, Liangxing Wang
Pulmonary arterial hypertension (PAH) is a devastating disease characterized by high pulmonary artery pressure. It is reported that microRNA-204 (miR-204) plays an important role in the development of PAH. Calcitriol [1,25-dihydroxyvitamin D3, 1,25(OH)2D3] mediates multiple pathophysiological processes. The aim of the current study was to explore the role of 1,25(OH)2D3 in PAH. PAH was induced in rats and rat pulmonary arterial endothelial cells (PAECs) were isolated as in vitro PAH model. The mean pulmonary artery pressure, morphologic changes, and expressions of transforming growth factor-beta1 (Tgfbr2), Smad2/7, alpha smooth muscle actin (α-SMA), and p21 were then measured...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196165/osteopontin-promotes-collagen-i-synthesis-in-hepatic-stellate-cells-by-mirna-129-5p-inhibition
#16
Yinghua Chen, Yitao Ou, Jiale Dong, Guizhi Yang, Zhi Zeng, Ying Liu, Bing Liu, Weidong Li, Xiaoshun He, Tian Lan
Activation of hepatic stellate cells (HSCs) is an essential event in the initiation and progression of liver fibrosis. HSCs are believed to be the major source of collagen-producing myofibroblasts in fibrotic livers. A key feature in the pathogenesis of liver fibrosis is fibrillar Collagen I (Col 1) deposition. Osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed in HSCs, could drive fibrogenesis by modulating the HSC pro-fibrogenic phenotype and Col 1 expression. Here, we aimed to investigate the molecular mechanism of OPN regulating the activation of HSCs...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196164/glucocorticoids-indirectly-decrease-colon-cancer-cell-proliferation-and-invasion-via-effects-on-cancer-associated-fibroblasts
#17
Zuzanna Drebert, Elly De Vlieghere, Jolien Bridelance, Olivier De Wever, Karolien De Bosscher, Marc Bracke, Ilse M Beck
Cancer-associated fibroblasts (CAFs) support cancer growth, invasion, and metastasis. Glucocorticoids (GCs), drugs often administered together with chemotherapy, are steroidal ligands of the glucocorticoid receptor (GR), a transcription factor which upon activation regulates expression of multiple genes involved in suppression of inflammation. We have previously shown that in dexamethasone (Dex)-treated CAFs derived from colon cancer, production and secretion of several factors related to cancer progression, such as tenascin C (TNC) and hepatocyte growth factor (HGF), were strongly suppressed...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29196163/the-mir-182-sort1-axis-regulates-vascular-smooth-muscle-cell-calcification-in-vitro-and-in-vivo
#18
Zhanman Zhang, Wenhong Jiang, Han Yang, Qiuning Lin, Xiao Qin
Arterial calcification is a common feature of cardiovascular disease. Sortilin is involved in the development of atherosclerosis, but the specific mechanism is unclear. In this study, we established calcification models in vivo and in vitro by using vitamin D3 and β-glycerophosphate, respectively. In vivo, the expression of SORT1 was up-regulated and the expression of miR-182 was down-regulated in calcified arterial tissues. Meanwhile there was a negative correlation between SORT1 expression and miR-182 levels...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29191552/production-of-bioactive-recombinant-human-eb-peptide-of-pro-igf-i-and-identification-of-binding-components-from-the-plasma-membrane-of-human-breast-cancer-cells-mda-mb-231
#19
Jay H Lo, Thomas T Chen
E-peptide of the pro-Insulin-like growth factor-I (pro-IGF-I) is produced from pre-pro-IGF-I by proteolytic cleavage in the post-translational processing. The human Eb-peptide (hEb-peptide), derived from the E domain of pro-IGF-IB isoform, is a bioactive molecule whose exact physiological role remains elusive. Accumulated evidence reported from our laboratory indicated that hEb-peptide possesses activity against multiple hallmark characteristics of solid tumor in different cancer cell types. In human breast carcinoma cells (MDA-MB-231), it was demonstrated that hEb-peptide can promote cell attachment to substratum, inhibit colony formation in a semisolid medium, reduce cancer cell invasion, and inhibit cancer-induced angiogenesis...
November 27, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29179977/altered-o-glycosylation-is-associated-with-inherent-radioresistance-and-malignancy-of-human-laryngeal-carcinoma
#20
Xiaoxia Dong, Zhiguo Luo, Yingying Wang, Lu Meng, Qiwen Duan, Li Qiu, Feng Peng, Li Shen
Radioresistance (inherent or acquired) remains a major obstacle affecting the clinical outcome of radiotherapy for laryngeal carcinoma. Results from our laboratory and other groups suggest that aberrant glycosylation contributes to cancer acquired radioresistance. However, the role of glycosylation in inherent radioresistance of laryngeal carcinoma has not been fully uncovered. In this study, we investigated the glycan profiling of the inherent radioresistant (Hep-2max) and radiosensitive (Hep-2min) cell lines using lectin microarray analysis...
November 26, 2017: Experimental Cell Research
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