journal
https://read.qxmd.com/read/38537746/dna-damage-regulated-autophagy-modulator-1-prevents-glioblastoma-cells-proliferation-by-regulating-lysosomal-function-and-autophagic-flux-stability
#1
JOURNAL ARTICLE
Hongqiang Zhang, Lan Luan, Xinyu Li, Xu Sun, Kang Yang
Glioblastoma (GBM) is the most aggressive and life-threatening brain tumor, characterized by its highly malignant and recurrent nature. DNA damage-regulated autophagy modulator 1 (DRAM-1) is a p53 target gene encoding a lysosomal protein that induces macro-autophagy and damage-induced programmed cell death in tumor growth. However, the precise mechanisms underlying how DRAM-1 affects tumor cell proliferation through regulation of lysosomal function and autophagic flux stability remain incompletely understood...
March 25, 2024: Experimental Cell Research
https://read.qxmd.com/read/38537745/young-human-plasma-derived-extracellular-vesicles-rescue-and-reactivate-il-1%C3%AE-and-tnf-%C3%AE-treated-chondrocytes
#2
JOURNAL ARTICLE
Rongjie Wu, Yu Xie, Yujie Peng, Xiaohu Wu, Yuanchen Ma, Feng-Juan Lyu, Qiujian Zheng, Zhantao Deng
Osteoarthritis (OA) is a degenerative disease that affects millions of individuals worldwide. Despite its prevalence, the exact causes and mechanisms behind OA are still not fully understood, resulting in a lack of effective treatments to slow down or halt disease progression. Recent research has discovered that extracellular vesicles (EVs) present in the circulation of young mice have a remarkable ability to activate musculoskeletal stem cells in elderly mice. Conversely, EVs derived from elderly mice do not exhibit the same potential, indicating that EVs obtained from young individuals may hold promise to activate aging cells in degenerative tissue...
March 25, 2024: Experimental Cell Research
https://read.qxmd.com/read/38531506/decoding-the-epigenetic-mechanism-of-mammalian-sex-determination-updates-emerging-hypothesis-and-unsolved-questions
#3
REVIEW
Cash Kumar, Jagat Kumar Roy
Sex determination embodies a dynamic and intricate developmental process wielding significant influence over the destiny of bipotential gonads, steering them towards male or female gonads. Gonadal differentiation and the postnatal manifestation of the gonadal phenotype involve a sophisticated interplay of transcription factors such as SOX9 and FOXL2. Central to this interplay are chromatin modifiers regulating the mutual antagonism during this interplay. In this review, the key findings and knowledge gaps in DNA methylation, histone modification, and non-coding RNA-mediated control throughout mammalian gonadal development are covered...
March 24, 2024: Experimental Cell Research
https://read.qxmd.com/read/38513962/protein-phosphatase-4-mediates-palmitic-acid-induced-endothelial-dysfunction-by-decreasing-enos-phosphorylation-at-serine-633-in-huvecs
#4
JOURNAL ARTICLE
Zhengwei Liang, Gang Sun, Junshi Zhang, Qian Zhang, Xiaoyu Li, Si Qin, Sha Lv, Jing Ding, Qifang Zhang, Yong Xia, Deqin Lu
Plasma saturated free fatty acid (FFA)-induced endothelial dysfunction (ED) contributes to the pathogenesis of atherosclerosis and cardiovascular diseases. However, the mechanism underlying saturated FFA-induced ED remains unclear. This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein phosphatase 2A (PP2A), thereby reducing endothelial nitric oxide synthase (eNOS) phosphorylation at Ser633 and Ser1177, respectively...
March 19, 2024: Experimental Cell Research
https://read.qxmd.com/read/38508329/tmem158-functions-as-an-oncogene-and-promotes-lung-adenocarcinoma-progression-through-the-pi3k-akt-pathway-via-interaction-with-twist1
#5
JOURNAL ARTICLE
Tao Xu, Fang Yin, Kaihu Shi
Lung adenocarcinoma (LUAD) is a common and deadly form of lung cancer, with high rates of metastasis and unsatisfactory clinical outcomes. Herein, we examined the influence of TMEM158 on the LUAD progression. A combination of bioinformatic analyses was used to assess the TMEM158 expression pattern, prognostic implications, and potential function in LUAD. The levels of TMEM158 and TWIST1 were evaluated in clinical samples from LUAD patients using Western blot analysis and qRT-PCR. To discover the function and underlying molecular pathways of TMEM158 in LUAD, we employed a combination of experimental approaches in vitro, such as flow cytometry analysis and colony formation, Co-IP, CCK-8, Transwell, and wound-healing assays...
March 18, 2024: Experimental Cell Research
https://read.qxmd.com/read/38508328/tgf-%C3%AE-egfr-signaling-promotes-lipopolysaccharide-induced-abnormal-elastin-deposition-and-alveolar-simplification
#6
JOURNAL ARTICLE
Jianhui Li, Jian Cao, Chongbing Yan, Xiaohui Gong
Bronchopulmonary dysplasia (BPD) is characterized by shortened secondary septa and fewer, larger alveoli. Elastin deposition to the distal tips of the secondary septa is critical for elongation of the secondary septa. Alveolar myofibroblasts, which are thought to migrate to the septal tips during alveolarization, are mainly responsible for elastin production and deposition. Antenatal exposure to inflammation induces abnormal elastin deposition, thereby increasing the risk of developing BPD. Here, we found that lipopolysaccharide (LPS) significantly increased the expression of transforming growth factor-α (TGF-α) in an LPS-induced rat model of BPD and in LPS-treated human pulmonary epithelial cells (BEAS-2B)...
March 18, 2024: Experimental Cell Research
https://read.qxmd.com/read/38508327/inhibition-of-pd-l1-expression-in-non-small-cell-lung-cancer-may-reduce-vasculogenic-mimicry-formation-by-inhibiting-the-epithelial-mesenchymal-transformation-process
#7
JOURNAL ARTICLE
Yuqi Shi, Wenjuan Li, Qianhao Jia, Jiatao Wu, Shoufan Wu, Shiwu Wu
Non-small cell lung cancer (NSCLC) is a kind of highly malignant tumor. Studies have shown that Vasculogenic mimicry (VM) may be responsible for dismal prognosis in NSCLC. Immunotherapy with programmed death-1 (PD-1) or programmed death ligand-1 (PD-L1) has significantly altered the treatment of assorted cancers, including NSCLC, but its role and mechanism in the formation of Vasculogenic mimicry (VM) in NSCLC remains unclear. This study aimed to investigate the role of the anti-PD-L1 antibody in the formation of VM in NSCLC and its possible mechanisms...
March 18, 2024: Experimental Cell Research
https://read.qxmd.com/read/38499143/a-comparison-between-different-human-hepatocyte-models-reveals-profound-differences-in-net-glucose-production-lipid-composition-and-metabolism-in-vitro
#8
JOURNAL ARTICLE
Flavio Bonanini, Madhulika Singh, Hong Yang, Dorota Kurek, Amy C Harms, Adil Mardinoglu, Thomas Hankemeier
Hepatocytes are responsible for maintaining a stable blood glucose concentration during periods of nutrient scarcity. The breakdown of glycogen and de novo synthesis of glucose are crucial metabolic pathways deeply interlinked with lipid metabolism. Alterations in these pathways are often associated with metabolic diseases with serious clinical implications. Studying energy metabolism in human cells is challenging. Primary hepatocytes are still considered the golden standard for in vitro studies and have been instrumental in elucidating key aspects of energy metabolism found in vivo...
March 16, 2024: Experimental Cell Research
https://read.qxmd.com/read/38499142/klf10-cbs-increases-the-sensitivity-of-gastric-carcinoma-cells-to-methionine-restriction-by-promoting-sulfur-transfer-pathway
#9
JOURNAL ARTICLE
Lin Xin, Zhi-Yang Liu, Chen-Xi Liu, Jie Sheng, Qi Zhou, Yi-Wu Yuan, Zhen-Qi Yue, Hao Lu, Jin-Heng Gan
Gastric cancer metastasis is a major cause of poor prognosis. Our previous research showed that methionine restriction (MR) lowers the invasiveness and motility of gastric carcinoma. In this study, we investigated the particular mechanisms of MR on gastric carcinoma metastasis. In vitro, gastric carcinoma cells (AGS, SNU-5, MKN7, KATO III, SNU-1, and MKN45) were grown in an MR medium for 24 h. In vivo, BALB/c mice were given a methionine-free (Met- ) diet. Transwell assays were used to investigate cell invasion and migration...
March 16, 2024: Experimental Cell Research
https://read.qxmd.com/read/38494067/regulation-of-the-ages-induced-inflammatory-response-in-human-periodontal-ligament-cells-via-the-ampk-nf-%C3%AE%C2%BAb-nlrp3-signaling-pathway
#10
JOURNAL ARTICLE
Zhu-Ling Guo, Jie Zhou, Xue-Jing Lin, Qing Yuan, Yu-Lei Dong, Qi-Bing Liu, Tao Wang
The heightened prevalence and accelerated progression of periodontitis in individuals with diabetes is primarily attributed to inflammatory responses in human periodontal ligament cells (HPDLCs). This study is aimed at delineating the regulatory mechanism of nucleotide-binding oligomerization domain-like receptors (NLRs) in mediating inflammation incited by muramyl dipeptide (MDP) in HPDLCs, under the influence of advanced glycation end products (AGEs), metabolic by-products associated with diabetes. We performed RNA-seq in HPDLCs induced by AGEs treatment and delineated activation markers for the receptor of AGEs (RAGE)...
March 15, 2024: Experimental Cell Research
https://read.qxmd.com/read/38492634/notoginsenoside-r1-targets-ppar-%C3%AE-to-inhibit-hepatic-stellate-cell-activation-and-ameliorates-liver-fibrosis
#11
JOURNAL ARTICLE
Cheng Guo, Linying Lai, Boyu Ma, Qian Huang, Zhirong Wang
BACKGROUND: Hepatic fibrosis, a common pathological process that occurs in end-stage liver diseases, is a serious public health problem and lacks effective therapy. Notoginsenoside R1 (NR1) is a small molecule derived from the traditional Chinese medicine Sanqi, exhibiting great potential in treating diverse metabolie disorders. Here we aimed to enquired the role of NR1 in liver fibrosis and its underlying mechanism in hepatoprotective effects. METHODS: We investigated the anti-fibrosis effects of NR1 using CCl4 -induced mouse mode of liver fibrosis as well as TGF-β1-activated JS-1, LX-2 cells and primary hepatic stellate cells...
March 14, 2024: Experimental Cell Research
https://read.qxmd.com/read/38490621/targeting-inhibitor-of-apoptosis-proteins-iaps-enhances-susceptibility-of-oral-squamous-carcinoma-cells-to-cisplatin
#12
JOURNAL ARTICLE
Tianyi Gao, Stefania Magnano, Amy Rynne, Lucy O'Kane, Patricia Hannon Barroeta, Daniela M Zisterer
PURPOSE: Oral Squamous Cell Carcinoma (OSCC) is the 6th most common cancer worldwide. It is generally aggressive and closely associated with chemoresistance and poor survival. There is accumulating evidence for the involvement of inhibitors of apoptosis proteins (IAPs), including IAP1 and XIAP, in mediating chemotherapy resistance in OSCC. Various strategies for targeting IAPs have been designed and tested in recent years and several small molecule IAP inhibitors are in clinical trials as monotherapies as well as in combination with radiotherapy and chemotherapy...
March 13, 2024: Experimental Cell Research
https://read.qxmd.com/read/38485079/single-cell-tracking-as-a-tool-for-studying-emt-phenotypes
#13
JOURNAL ARTICLE
Ellen Marie Botne Quinsgaard, Mónica Suárez Korsnes, Reinert Korsnes, Siver Andreas Moestue
This article demonstrates that label-free single-cell video tracking is a useful approach for in vitro studies of Epithelial-Mesenchymal Transition (EMT). EMT is a highly heterogeneous process, involved in wound healing, embryogenesis and cancer. The process promotes metastasis, and increased understanding can aid development of novel therapeutic strategies. The role of EMT-associated biomarkers depends on biological context, making it challenging to compare and interpret data from different studies. We demonstrate single-cell video tracking for comprehensive phenotype analysis...
March 12, 2024: Experimental Cell Research
https://read.qxmd.com/read/38479704/alkbh5-mediated-upregulation-of-cpt1a-promotes-macrophage-fatty-acid-metabolism-and-m2-macrophage-polarization-facilitating-malignant-progression-of-colorectal-cancer
#14
JOURNAL ARTICLE
Mingming Sun, Yinzi Yue, Xiaopeng Wang, Huayi Feng, Yuanyuan Qin, Mengyao Chen, Yahui Wang, Shuai Yan
m6A modification has been studied in tumors, but its role in host anti-tumor immune response and TAMs polarization remains unclear. The fatty acid oxidation (FAO) process of TAMs is also attracting attention. A co-culture model of colorectal cancer (CRC) cells and macrophages was used to simulate the tumor microenvironment. Expression changes of m6A demethylase genes FTO and ALKBH5 were screened. ALKBH5 was further investigated. Gain-of-function experiments were conducted to study ALKBH5's effects on macrophage M2 polarization, CRC cell viability, proliferation, migration, and more...
March 11, 2024: Experimental Cell Research
https://read.qxmd.com/read/38462209/high-level-27-hc-impairs-trophoblast-cell-invasion-and-migration-via-lxr-in-pre-eclampsia
#15
JOURNAL ARTICLE
Hai Tong, Tingting Wang, Ruixin Chen, Pingsong Jin, Youqing Jiang, Xue Zhang, Hongbo Qi
INTRODUCTION: To explore the potential impact of 27-hydroxycholesterol (27-HC) on trophoblast cell function in pre-eclampsia. RESULTS: The levels of 27-HC and the expression of CYP27A1 are upregulated in clinical samples of PE. Furthermore, high concentrations of 27-HC can inhibit the invasion and migration ability of trophoblast cells in vitro, and this inhibitory effect is weakened after LXR silencing. In HTR8/SVneo cells treated with 27-HC, the expression of ABCA1/ABCG1 are increased...
March 8, 2024: Experimental Cell Research
https://read.qxmd.com/read/38462208/the-kdm5-inhibitor-pbit-reduces-proliferation-of-castration-resistant-prostate-cancer-cells-via-cell-cycle-arrest-and-the-induction-of-senescence
#16
JOURNAL ARTICLE
Tunde Smith, Tytianna White, Zhenbang Chen, LaMonica V Stewart
The compound 2-4(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT) is an inhibitor of the KDM5 family of lysine-specific histone demethylases that has been suggested as a lead compound for cancer therapy. The goal of this study was to explore the effects of PBIT within human prostate cancers. Micromolar concentrations of PBIT altered proliferation of castration-sensitive LNCaP and castration-resistant C4-2B, LNCaP-MDV3100 and PC-3 human prostate cancer cell lines. We then characterized the mechanism underlying the anti-proliferative effects of PBIT within the C4-2B and PC-3 cell lines...
March 8, 2024: Experimental Cell Research
https://read.qxmd.com/read/38462207/mettl3-promotes-trophoblast-ferroptosis-in-preeclampsia-by-stabilizing-the-acsl4-m-6-a-modification
#17
JOURNAL ARTICLE
Yang Wang, Gang Zhang, Yan Gao, Xuemei Zhang, Hongbo Qi
This study aims to explore the role of methyltransferase-like 3 (METTL3) modulation of ferroptosis in the pathogenesis of trophoblast-mediated preeclampsia. The expression of METTL3 and acyl-CoA synthetase long chain family member 4 (ACSL4) was measured in clinical placental tissues and trophoblasts using qPCR and Western blot techniques. The effects of METTL3 on the symptoms of preeclampsia were also validated in rat models. METTL3 and ACSL4 were upregulated in placental tissues from patients with preeclampsia and in hypoxia-induced trophoblasts...
March 8, 2024: Experimental Cell Research
https://read.qxmd.com/read/38432023/corrigendum-to-twist2-inhibits-emt-and-induces-oxidative-stress-of-lung-cancer-cells-by-regulating-fgf21-mediated-ampk-mtor-pathway-exp-cell-res-2021-aug-1-405-1-112661-ecr-21-80
#18
Yingjian Song, Wei Zhang, Jiuxu Zhang, Zhaolei You, Tao Hu, Guangyuan Shao, Zheng Zhang, Zhicheng Xu, Xiaofeng Yu
No abstract text is available yet for this article.
March 2, 2024: Experimental Cell Research
https://read.qxmd.com/read/38402662/corrigendum-to-mex3a-promotes-development-and-progression-of-breast-cancer-through-regulation-of-pik3ca-exp-cell-res-2021-jul-1-404-1-112580-epub-2021-apr-1
#19
Xianbiao Shi, Yulu Sun, Yin Zhang, Wei Wang, Jiahan Xu, Yinan Guan, Yitao Ding, Yongzhong Yao
No abstract text is available yet for this article.
February 24, 2024: Experimental Cell Research
https://read.qxmd.com/read/38401687/icbp90-an-epigenetic-regulator-induces-dkk3-promoter-methylation-promotes-glioma-progression-and-reduces-sensitivity-to-cis-platinum
#20
JOURNAL ARTICLE
Qinchen Cao, Xinxin Wang, Jie Liu, Yang Dong, Xiaolong Wu, Yin Mi, Ke Liu, Mingzhi Zhang, Yonggang Shi, Ruitai Fan
Glioma is the most common brain malignancy, characterized by high morbidity, high mortality, and treatment-resistance. Inverted CCAAT box Binding Protein of 90 kDa (ICBP90) has been reported to be involved in tumor progression and the maintenance of DNA methylation. Herein, we constructed ICBP90 over-expression and knockdown glioma cell lines, and found that ICBP90 knockdown inhibited glioma cell proliferation, migration, and invasion. ICBP90 silencing potentially enhanced cellular sensitivity to cis-platinum (DDP) and exacerbated DDP-induced pyroptosis, manifested by the elevated levels of gasdermin D-N-terminal and cleaved caspase 1; whereas, ICBP90 over-expression exhibited the opposite effects...
February 22, 2024: Experimental Cell Research
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