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Canadian Journal of Microbiology

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https://www.readbyqxmd.com/read/27848273/announcement-communiqu%C3%A3
#1
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27829287/the-influence-of-microbial-based-inoculants-on-n2o-emissions-from-soil-planted-with-corn-zea-mays-l-under-greenhouse-conditions-with-different-nitrogen-fertilizer-regimens
#2
Pamela Calvo, Dexter B Watts, Joseph W Kloepper, H Allen Torbert
Nitrous oxide (N2O) emissions are increasing at an unprecedented rate owing to the increased use of nitrogen (N) fertilizers. Thus, new innovative management tools are needed to reduce emissions. One potential approach is the use of microbial inoculants in agricultural production. In a previous incubation study, we observed reductions in N2O emissions when microbial-based inoculants were added to soil (no plants present) with N fertilizers under laboratory incubations. This present study evaluated the effects of microbial-based inoculants on N2O and carbon dioxide (CO2) emissions when applied to soil planted with corn (Zea mays L...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27822959/seasonal-changes-in-bacterial-communities-associated-with-healthy-and-diseased-porites-coral-in-southern-taiwan
#3
Chorng-Horng Lin, Chih-Hsiang Chuang, Wen-Hung Twan, Shu-Fen Chiou, Tit-Yee Wong, Jong-Kang Liu, Chyuan-Yao Kao, Jimmy Kuo
We compared the bacterial communities associated with healthy scleractinian coral Porites sp. with those associated with coral infected with pink spot syndrome harvested during summer and winter from waters off the coast of southern Taiwan. Members of the bacterial community associated with the coral were characterized by means of denaturing gradient gel electrophoresis (DGGE) of a short region of the 16S rRNA gene and clone library analysis. Of 5 different areas of the 16S rRNA gene, we demonstrated that the V3 hypervariable region is most suited to represent the coral-associated bacterial community...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27805417/prevalence-of-cry2-type-genes-in-bacillus-thuringiensis-isolates-recovered-from-diverse-habitats-in-india-and-isolation-of-a-novel-cry2af2-gene-toxic-to-helicoverpa-armigera-cotton-boll-worm
#4
Jawahar Lal Katara, Sarvjeet Kaur, Gouthami Krishna Kumari, Nagendra Kumar Singh
Insecticidal cry and vip genes from Bacillus thuringiensis (Bt) have been used for control of lepidopteran insects in transgenic crops. However, novel genes are required for gene pyramiding to delay evolution of resistance to the currently deployed genes. Two PCR-based techniques were employed for screening of cry2-type genes in 129 Bt isolates from diverse habitats in India and 27 known Bt strains. cry2Ab-type genes were more prevalent than cry2Aa- and cry2Ac-type genes. Correlation between source of isolates and abundance of cry2-type genes was not observed...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27805413/in-situ-identification-and-quantification-of-protein-hydrolyzing-ruminal-bacteria-associated-with-the-digestion-of-barley-and-corn-grain
#5
Yun Xia, Yunhong Kong, Heping Huang, Hee Eun Yang, Robert Forster, Tim A McAllister
In this study, BODIPY FL DQ™ casein staining combined with fluorescence in situ hybridization (FISH) was used to detect and identify protein-hydrolyzing bacteria within biofilms that produced active cell-surface-associated serine- and metallo-proteases during the ruminal digestion of barley and corn grain in cows fed barley-based diets at 2 different levels. A doublet coccoid bacterial morphotype associated with barley and corn grain particles fluoresced after BODIPY FL DQ™ casein staining. Bacteria with this morphotype accounted for 3%-10% of the total bacteria attached to surface of cereal grain particles, possibly indicative of an important role in the hydrolysis of the protein matrix within the endosperm...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27784163/variability-in-colonization-of-arbuscular-mycorrhizal-fungi-and-its-effect-on-mycorrhizal-dependency-of-improved-and-unimproved-soybean-cultivars
#6
M S Salloum, M C Guzzo, M S Velazquez, M B Sagadin, C M Luna
Breeding selection of germplasm under fertilized conditions may reduce the frequency of genes that promote mycorrhizal associations. This study was developed to compare variability in mycorrhizal colonization and its effect on mycorrhizal dependency (MD) in improved soybean genotypes (I-1 and I-2) with differential tolerance to drought stress, and in unimproved soybean genotypes (UI-3 and UI-4). As inoculum, a mixed native arbuscular mycorrhizal fungi (AMF) was isolated from soybean roots, showing spores mostly of the species Funneliformis mosseae...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27781484/molecular-characterization-of-pathogenic-and-nonpathogenic-fowl-aviadenovirus-serotype-11-isolates
#7
Patrick D Slaine, James G Ackford, Andrew M Kropinski, Robert A Kozak, Peter J Krell, Éva Nagy
Fowl aviadenoviruses, many of which are of importance in veterinary medicine, are classified into 5 species. In this study, a pathogenic isolate and a nonpathogenic isolate of fowl aviadenovirus serotype 11 (FAdV-11) of species Fowl aviadenovirus D were characterized. Growth rates were analyzed for the 2 isolates, showing notable differences. The complete genomic sequences of the viruses were fully determined and were analyzed. The genomes of the 2 isolates showed 98.1% sequence identity and revealed 6 nonsynonymous mutations between the Ontario isolates...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27763775/reliability-of-haemophilus-influenzae-biofilm-measurement-via-static-method-and-determinants-of-in-vitro-biofilm-production
#8
Najla A Obaid, Stephen Tristram, Christian K Narkowicz, Glenn A Jacobson
Information is lacking regarding the precision of microtitre plate (MTP) assays used to measure biofilm. This study investigated the precision of an MTP assay to measure biofilm production by nontypeable Haemophilus influenzae (NTHi) and the effects of frozen storage and inoculation technique on biofilm production. The density of bacterial final growth was determined by absorbance after 18-20 h incubation, and biofilm production was then measured by absorbance after crystal violet staining. Biofilm formation was categorised as high and low for each strain...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27759425/investigating-the-ability-of-pseudomonas-fluorescens-uw4-to-reduce-cadmium-stress-in-lactuca-sativa-via-an-intervention-in-the-ethylene-biosynthetic-pathway
#9
Lucas J Albano, Sheila M Macfie
A typical plant response to any biotic or abiotic stress, including cadmium (Cd), involves increased ethylene synthesis, which causes senescence of the affected plant part. Stressed plants can experience reduced ethylene and improved growth if they are inoculated with bacteria that have the enzyme ACC deaminase, which metabolizes the ethylene precursor ACC (1-aminocyclopropane-1-carboxylate). We investigated whether one such bacterium, Pseudomonas fluorescens UW4, reduces the production of ethylene and improves the growth of lettuce (Lactuca sativa) sown in Cd-contaminated potting material (PRO-MIX® BX)...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27718589/increased-glucose-utilization-and-cell-growth-of-corynebacterium-glutamicum-by-modifying-the-glucose-specific-phosphotransferase-system-pts-glc-genes
#10
Jianzhong Xu, Junlan Zhang, Dongdong Liu, Weiguo Zhang
The phosphoenolpyruvate:glucose phosphotransferase system (PTS(Glc)) is the major pathway of glucose uptake in Corynebacterium glutamicum. This study investigated glucose consumption rate, cell growth, and metabolite changes resulting from modification of PTS(Glc). The classical l-lysine producer C. glutamicum XQ-8 exhibited low glucose consumption, cell growth, and l-lysine production rates, whereas these parameters were significantly increased during cultivating on glucose plus maltose, through inactivation of SugR, or by overexpression of PTS(Glc) genes...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27771975/does-it-take-a-community-to-raise-a-plant-a-summary-of-the-canadian-crop-microbiome-workshop
#11
Ivan J Oresnik, Lisette Mascarenhas, Christopher K Yost
No abstract text is available yet for this article.
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27622981/erg11-mutations-and-upregulation-in-clinical-itraconazole-resistant-isolates-of-candida-krusei
#12
Wenli Feng, Jing Yang, Yiru Wang, Jinyu Chen, Zhiqin Xi, Zusha Qiao
To better understand the association between the ERG11 gene and drug resistance in Candida krusei, C. krusei strains were isolated from patients from January 2010 to May 2013. Susceptibility to 5-fluorocytosine (5-FC), amphotericin B (AMB), voriconazole (VRC), fluconazole (FLC), and itraconazole (ITR) were tested by broth microdilution method. Mutations were detected using PCR amplification and gene sequencing. Expression levels of ERG11 were measured by real-time PCR and compared by a 2-tailed Student's t test between ITR-susceptible strains and ITR-resistant strains...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27590823/effect-of-a-static-magnetic-field-on-escherichia-coli-adhesion-and-orientation
#13
Lotfi Mhamdi, Nejib Mhamdi, Naceur Mhamdi, Philippe Lejeune, Nicole Jaffrezic, Nöel Burais, Riccardo Scorretti, Jiry Pokorny, Laurence Ponsonnet
This preliminary study focused on the effect of exposure to 0.5 T static magnetic fields on Escherichia coli adhesion and orientation. We investigated the difference in bacterial adhesion on the surface of glass and indium tin oxide-coated glass when exposed to a magnetic field either perpendicular or parallel to the adhesion surface (vectors of magnetic induction are perpendicular or parallel to the adhesion surface, respectively). Control cultures were simultaneously grown under identical conditions but without exposure to the magnetic field...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27579995/revival-of-saprotrophic-and-mycorrhizal-basidiomycete-cultures-after-30-years-in-cold-storage-in-sterile-water
#14
Dana L Richter, Thomas G Dixon, Jill K Smith
Vegetatively colonized agar cores of 54 basidiomycete fungal isolates were stored at 5 °C in tubes of sterile distilled water without manipulation for 30 years. The cultures represented 28 isolates of saprotrophic fungi and 26 isolates of mycorrhizal fungi. These cultures came from a group of 57 fungal isolates that were determined to be viable after 20 years of cold-water storage. Overall, 47 of the 54 isolates (87%) grew vigorously when revived after storage for 30 years. Of the 28 saprotrophic fungal isolates, 26 revived (93%); of the 26 mycorrhizal fungal isolates, 21 revived (81%)...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27564086/diversity-of-culturable-bacteria-recovered-from-pico-bol%C3%A3-var-s-glacial-and-subglacial-environments-at-4950-m-in-venezuelan-tropical-andes
#15
Johnma Rondón, Wileidy Gómez, María M Ball, Alejandra Melfo, Marcos Rengifo, Wilvis Balcázar, Delsy Dávila-Vera, Alirio Balza-Quintero, Rosa Virginia Mendoza-Briceño, Luis Andrés Yarzábal
Even though tropical glaciers are retreating rapidly and many will disappear in the next few years, their microbial diversity remains to be studied in depth. In this paper we report on the biodiversity of the culturable fraction of bacteria colonizing Pico Bolívar's glacier ice and subglacial meltwaters, at ∼4950 m in the Venezuelan Andean Mountains. Microbial cells of diverse morphologies and exhibiting uncompromised membranes were present at densities ranging from 1.5 × 10(4) to 4.7 × 10(4) cells/mL in glacier ice and from 4...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27556366/insight-into-the-kluyveromyces-lactis-pdr1p-regulon
#16
Nora Toth Hervay, Alexandra Konecna, Zuzana Balazfyova, Alexandra Svrbicka, Yvetta Gbelska
The overexpression of efflux pumps is an important mechanism leading to the development of multidrug resistance phenomenon. The transcription factor KlPdr1p, belonging to the Zn2Cys6 family, is a central regulator of efflux pump expression in Kluyveromyces lactis. To better understand how KlPDR1-mediated drug resistance is achieved in K. lactis, we used DNA microarrays to identify genes whose expression was affected by deletion or overexpression of the KlPDR1 gene. Eighty-nine targets of the KlPDR1 were identified...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27510302/characterization-of-two-luxi-r-homologs-in-pantoea-ananatis-lmg-2665-t
#17
Siphathele Sibanda, Jacques Theron, Divine Y Shyntum, Lucy N Moleleki, Teresa A Coutinho
Quorum sensing (QS) plays an important role in the regulation of bacteria-host interactions and ecological fitness in many bacteria. In this study, 2 luxI/R homologs, namely eanI/eanR and rhlI/rhlR, were identified in the genome sequence of Pantoea ananatis LMG 2665(T). To determine a role for these luxI/R homologs in pathogenicity and biofilm formation, mutant bacterial strains lacking either eanI/R or rhlI/R and both of these homologs were generated. The results indicated that both the RhlI/R and EanI/R systems are required for pathogenicity and biofilm formation in strain LMG 2665(T)...
November 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27604298/termitarium-inhabiting-bacillus-endophyticus-tsh42-and-bacillus-cereus-tsh77-colonizing-curcuma-longa-l-isolation-characterization-and-evaluation-of-their-biocontrol-and-plant-growth-promoting-activities
#18
Ankit Kumar Chauhan, Dinesh Kumar Maheshwari, Kangmin Kim, Vivek K Bajpai
Bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. Further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. Termitarium-inhabiting Bacillus strains TSH42 and TSH77 significantly reduced the growth of pathogenic fungus Fusarium solani, controlled the symptoms of rhizome rot in turmeric (Curcuma longa L...
October 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27590666/a-review-of-techniques-for-detecting-huanglongbing-greening-in-citrus
#19
Roberto Arredondo Valdés, Juan C Delgado Ortiz, Mariana Beltrán Beache, Julia Anguiano Cabello, Ernesto Cerna Chávez, Yolanda Rodríguez Pagaza, Yisa M Ochoa Fuentes
Huanglongbing (HLB) is the most destructive disease of citrus worldwide. Monitoring of health and detection of diseases in trees is critical for sustainable agriculture. HLB symptoms are virtually the same wherever the disease occurs. The disease is caused by Candidatus Liberibacter spp., vectored by the psyllids Diaphorina citri Kuwayama and Trioza erytreae. Electron microscopy was the first technique used for HLB detection. Nowadays, scientists are working on the development of new techniques for a rapid HLB detection, as there is no sensor commercially accessible for real-time assessment of health conditions in trees...
October 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27556282/enriching-acid-rock-drainage-related-microbial-communities-from-surface-deposited-oil-sands-tailings
#20
Courtney Dean, Yeyuan Xiao, Deborah J Roberts
Little is known about the microbial communities native to surface-deposited pyritic oil sands tailings, an environment where acid rock drainage (ARD) could occur. The goal of this study was to enrich sulfur-oxidizing organisms from these tailings and determine whether different populations exist at pH levels 7, 4.5, and 2.5. Using growth-based methods provides model organisms for use in the future to predict potential activities and limitations of these organisms and to develop possible control methods. Thiosulfate-fed enrichment cultures were monitored for approximately 1 year...
October 2016: Canadian Journal of Microbiology
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