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Canadian Journal of Microbiology

Ashok Bankar, Smita Zinjarde, Aishwarya Telmore, Aishwarya Walke, Ameeta Ravikumar
The marine dimorphic yeast, Y. lipolytica has been proposed as a suitable model for the dimorphism study. In this study, the morphological behaviour of two marine strains of Y. lipolytica (NCIM 3589 and NCIM 3590) was studied under stress of different heavy metals. The scanning electron microscope (SEM) was used to investigate the morphological features of yeast cells. This study revealed that the normal ellipsoidal shape of yeast cells was changed into oval, rounded, or elongated ones in response to different heavy metal-stress...
April 24, 2018: Canadian Journal of Microbiology
Joost Theo Petra Verhoeven, Marta Canuti, Hannah J Munro, Suzanne C Dufour, Andrew S Lang
High-throughput sequencing (HTS) technologies are becoming increasingly important within microbiology research, but aspects of library preparation, such as high cost per sample or strict input requirements, make HTS difficult to implement in some niche applications and for research groups on a budget. To answer these necessities, we developed ViDiT, a customizable, PCR-based, extremely low-cost (<5 US dollars per sample) and versatile library preparation method, and CACTUS, an analysis pipeline designed to rely on cloud computing power to generate high-quality data from ViDiT-based experiments without the need of expensive servers...
April 19, 2018: Canadian Journal of Microbiology
Karlee Fischer, Mikayla Roberts, Scott Roscoe, Yasin Avci, Sirinart Ananvoranich
To gain an insight into the mechanisms by which <i>Toxoplasma gondii</i> interacts with the host cells at the molecular level, human poly-(A) binding protein (PABP) is being investigated. PABP family is an indicator of translation in the cell, given its roles in mRNA stability, translational initiation and repression. Following a heat shock, the formation of PABP-containing granules was observed in both the cytoplasm and nucleus of human fibroblasts. This heat shock response does not affect the progression of <i>T...
April 15, 2018: Canadian Journal of Microbiology
Walid Ellouze, Chantal Hamel, Asheesh K Singh, Vachaspati Mishra, Ron DePauw, Ron Knox
Understanding the variation in how wheat genotypes shape their arbuscular mycorrhizal (AM) fungal communities in a prairie environment is foundational to breeding for enhanced AM fungi-wheat interactions. The AM fungal communities associated with 32 durum wheat genotypes were described by pyrosequencing of amplicons. The experiment was set up at two locations in the Canadian prairies. The intensively managed site was highly dominated by Funneliformis. Genotype influenced the AM fungal community in the rhizosphere soil, but there was no evidence of a differential genotype effect on the AM fungal community of durum wheat roots...
April 10, 2018: Canadian Journal of Microbiology
Adharsh Rajasekar, Sekar Raju, Eduardo Medina-Roldan, Jonathan Bridge, Charles K S Moy, Stephen Wilkinson
The impact of contaminated leachate on groundwater from landfills is well known but specific effects on bacterial consortia are less well-studied. Bacterial communities in landfill and an urban site located in Suzhou, China were studied using Illumina high-throughput sequencing. A total number of 153944 good quality reads were produced and sequences assigned to 6388 operational taxonomic units (OTUs). Bacterial consortia consisted of up to 16 phyla including Proteobacteria (31.9 to 94.9% at landfill, 25.1 to 43...
April 10, 2018: Canadian Journal of Microbiology
Maria Daniela Artigas Ramirez, Jessica Danila Silva, Naoko Ohkama-Ohtsu, Tadashi Yokoyama
Aluminium (Al) toxicity is a major problem affecting soil fertility, microbial diversity, and nutrients uptake of plants. Rhizobia response and legume-interaction under Al conditions are still unknown and it is important to understand how to develop and improve legume cultivation under Al-stress. In this study, rhizobia-response was recorded under different Al-concentrations. Al-effect on rhizobial cells was characterized by combination with different two pH conditions. Symbiosis process was compared between α- and β-rhizobia inoculated onto soybean varieties...
April 5, 2018: Canadian Journal of Microbiology
Babafela Awosile, Greg J German, Juan Carlos Rodriguez-Lecompte, Matthew Elijah Saab, Luke C Heider, J Trenton McClure
The aim of this study was to determine the frequency of fecal carriage of vancomycin-resistant Enterococcus spp and Escherichia coli with reduced susceptibilities to extended-spectrum cephalosporins (ESCs) and quinolones in humans on Prince Edward Island, Canada. Convenience fecal samples from individuals on Prince Edward Island were screened phenotypically using selective culture and genotypically using multiplex polymerase chain reactions to detect E. coli and Enterococcus spp. resistant to critically important antimicrobials...
April 5, 2018: Canadian Journal of Microbiology
David Szalewski, Victoria S Hinrichs, Denise K Zinniel, Raúl G Barletta
The genus <i>Aspergillus</i> includes fungal species that cause major health issues of significant economic importance. These microorganisms are also the culprit for production of carcinogenic aflatoxins in grain storages, contaminating crops, and economically straining the production process. <i>Aspergillus fumigatus</i> is a very important pathogenic species, being responsible for high human morbidity and mortality on a global basis. The prevalence of these infections in immunosuppressed individuals is on the rise and physicians struggle with the diagnosis of these deadly pathogens...
March 27, 2018: Canadian Journal of Microbiology
Géssika Silva Souza, Lais Pessanha Carvalho, Edésio José Tenório de Melo, Valdirene Moreira Gomes, André de Oliveira Carvalho
Plant defensins are plant antimicrobial peptides that present diverse biological activities<i> in vitro</i>, including the elimination of <i>Leishmania amazonensis</i>. Plant defensins are considered promising candidates for the development of new drugs. This protozoan genus has great epidemiological importance and the mechanism behind the protozoan death by defensins is unknown, thus, we chose <i>L. amazonesis</i> for this study. The aim of the work was to analyze the possible toxic mechanisms of <i>Vu</i>-Defr against<i> L...
March 27, 2018: Canadian Journal of Microbiology
Amanda C Carroll, Alex Wong
Plasmids are extrachromosomal DNA elements that can be found throughout bacteria, as well as in other domains of life. Nonetheless, the evolutionary processes underlying the persistence of plasmids are incompletely understood. Bacterial plasmids may encode genes for traits which are sometimes beneficial to their hosts, such as antimicrobial resistance, virulence, heavy metal tolerance, and the catabolism of unique nutrient sources. In the absence of selection for these traits, however, plasmids generally impose a fitness cost on their hosts...
March 21, 2018: Canadian Journal of Microbiology
Wenxi Qian, Weiping Ao, Xiaohong Hui, Jianping Wu
The ruminal microbiota plays major roles in feed digestion. The composition and fermentation of the bacterial communities in three important ruminant species have been studied previously. Here, we extended this research to the effect of concentrate-to-forage ratios on ruminal bacterial communities in Tarim red deer (Cervus elaphus yarkandensis). Different concentrate-to-forage ratios [2:8, 3:7, 4:6 and 5:5] were fed to Tarim red deer for 20 days. Ruminal bacterial communities were elucidated by 16S ribosomal RNA gene sequencing on an Illumina HiSeq 2500 platform...
March 21, 2018: Canadian Journal of Microbiology
Luciana Ramos Almeida, Patrícia Silva Costa, Andréa M A Nascimento, Mariana Reis, Katharina Oliveira Barros, Luige Biciati Alvim, Alvaro Cantini Nunes, Dulciene Magalhães Queiroz, Gifone Aguiar Rocha, Jacques Robert Nicoli, Sílvia Beleza Moura
Although <i>Lactobacillus </i>species are recognized as normal inhabitants of porcine gastric mucosa, the association of these bacteria with health status or gastric ulcer disease has never been considered. We investigated the bacterial load of <i>Lactobacillus </i>isolated from the antrum, corpus and <i>pars esophagea</i> of stomachs with (n=13) and without (n=10) ulcer of the <i>pars esophagea</i> of slaughtered pigs. We also evaluated in vitro antagonistic properties against typical pathogens of strains isolated from stomachs without ulcer...
March 19, 2018: Canadian Journal of Microbiology
Urmi Bajpai, Abhishek Kumar Mehta, Kandasamy Eniyan, Avni Sinha, Ankita Ray, Simran Virdi, Shazeb Ahmad, Aridni Shah, Deepanksha Arora, Devyani Marwaha, Gunjan Chauhan, Prarthna Saraswat, Punita Bathla, Ruchi Singh
Bacteriophages are being considered as a promising natural resource for the development of alternative strategies against mycobacterial diseases, especially in the context of the wide spread occurrence of drug-resistance amongst the clinical isolates of M. tuberculosis. However, there isn't much information documented on mycobacteriophages from India. Here, we report the isolation of 17 mycobacteriophages using M. smegmatis as the bacterial host where 9 phages also lyse M. tuberculosis H37Rv. We present detailed analysis of one of these mycobacteriophage (PDRPv)...
March 15, 2018: Canadian Journal of Microbiology
Lingling Gan, Xuemei Zhang, Xiuyu Xu, Wenchun Xu, Chang Lu, Jin Cui, Hong Wang
<i>spd1672</i>, a novel <i>Streptococcus pneumoniae</i> (<i>S. pn</i>) gene induced in vivo, has been identified to contribute to the virulence of <i>S. pn</i>; however, the role of <i>spd1672</i> during host innate immune reaction against<i> S. pn</i> infection is unknown. In the present study, mice were infected with wild type D39 and mutant D39Δspd1672 strains. Compared to the D39-infected mice, reduced bacterial load and attenuated inflammatory response were observed in the D39Δ<i>spd1672</i>-treated mice...
March 15, 2018: Canadian Journal of Microbiology
Riyoko Tamai, Michiyo Kobayashi-Sakamoto, Yusuke Kiyoura
Galectin-1 and galectin-3 are C-type lectin receptors that bind to lipopolysaccharide (LPS) in the cell wall of gram-negative bacteria. In this study, we investigated the effects of galectin-1 and galectin-3 on adhesion to and invasion of the human gingival epithelial cell line Ca9-22 by Porphyromonas gingivalis, a periodontal pathogenic gram-negative bacterium. Recombinant galectin-1, but not galectin-3, enhanced P. gingivalis adhesion and invasion, although both galectins bound similarly to P. gingivalis...
March 15, 2018: Canadian Journal of Microbiology
Jie Ren, Tingting Lian, Lili Shao, Yuanjun Liu, Quanzhong Liu
Chlamydia trachomatis (C. trachomatis) is the most common cause of bacterial sexually transmitted infections. The effect of antibiotic treatment is not satisfactory, and there is currently no vaccine to prevent C. trachomatis infection. Our results showed that Chlamydia virus CPG1 capsid protein Vp1 treatment significantly inhibited C. trachomatis growth in cell culture, and the inclusion numbers of different C. trachomatis serotypes were decreased. In addition, we conducted a preliminary investigation of the possible mechanisms behind the Vp1 inhibition effects and C...
March 6, 2018: Canadian Journal of Microbiology
Patricia Rojas, Nuria Rodriguez, Vicenta de la Fuente, Daniel Sánchez-Mata, Ricardo Amils, Jose Luis Sanz Martin
Soda lakes are inhabited by important haloalkaliphilic microbial communities that are well adapted to these extreme characteristics. The surface waters of the haloalkaline Mono Lake (CA, USA) are alkaline but, in contrast to its bottom waters, do not present high salinity. We have studied the microbiota present in the shoreline sediments of Mono Lake using next-generation sequencing techniques. The statistical indexes showed that Bacteria had a higher richness, diversity and evenness compared to Archaea. 17 phyla and 8 "candidate divisions", were identified among the Bacteria, with a predominance of the phyla <i>Firmicutes</i>, <i>Proteobacteria </i>and <i>Bacteroidetes</i>...
March 6, 2018: Canadian Journal of Microbiology
Rhiannon L Wallace, Danielle L Hirkala, Louise M Nelson
The ability of <i>Pseudomonas fluorescens</i> isolates 1-112, 2-28, and 4-6, to control <i>Mucor piriformis</i> (Mucor rot) on 'Gala', 'McIntosh', 'Ambrosia', and 'Spartan' apples in commercial cold storage, and their possible mechanisms of action were investigated. Isolates 1-112 and 2-28, provided significant levels of disease control on 'McIntosh' and 'Spartan' apples, while isolate 4-6 provided control of Mucor rot on 'Gala' and 'Spartan' apples compared with control fruits after 15 weeks storage at 0°C...
March 5, 2018: Canadian Journal of Microbiology
Alexsander Moraes, Filipe C Cortelli, Taís B Miranda, Davi R Aquino, José R Cortelli, Maria Isabel A Guimarães, Fernando O Costa, Sheila C Cortelli
Transovarial transmission is among the reported factors able to influence environmental maintenance of dengue virus (DENV). Endemic areas with active transmission of dengue are suitable for studying transovarial transmission. Brazil is a country where dengue is endemic and where DENV-1 is the most common disease-related virus serotype. This study aimed at identifying transovarial transmission of DENV-1 in <i>Aedes aegypti</i> larvae using reverse-transcriptase nested real-time polymerase chain reaction (RT-nested-qPCR)...
March 5, 2018: Canadian Journal of Microbiology
Xiaoying Zhi, Jianliang Lv, Yanquan Wei, Yuan Gao, Ping Du, Yanyan Chang, Yun Zhang, Run Wu, Huichen Guo
No abstract text is available yet for this article.
February 28, 2018: Canadian Journal of Microbiology
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