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Canadian Journal of Microbiology

Kandaswamy Rekha, Baburaj Baskar, Santhanam Srinath, Balasundaram Usha
Malic acid, one of the major organic acid exudates from roots, plays a significant role in the chemotaxis of beneficial bacteria to the plant's rhizosphere. In this study, the effect of a plant growth promoting rhizobacterium, Bacillus subtilis RR4, on the synthesis and exudation of malic acid (MA) from roots is demonstrated in rice. To test the chemotactic ability of RR4 towards MA, capillary chemotaxis assay was performed which revealed a positive response (relative chemotactic ratio of 6.15 with 10 µM MA) and with increasing concentrations of MA, an elevated chemotactic response was observed...
October 18, 2017: Canadian Journal of Microbiology
Zhibo Cheng, Fenghua Zhang, William Jeffrey Gale, Weichao Wang, Wen Sang, Haichang Yang
The objective of this study was to evaluate bacterial community structure and diversity in soil aggregate fractions when salinized farmland was reclaimed after > 27 yr of abandonment and then farmed again for 1, 5, 10, and 15 yr. Illumina MiSeq high-throughput sequencing was performed to characterize the soil bacterial communities in five aggregate size classes in each treatment. The results indicated that reclamation significantly increased macro-aggregation (> 0.25 mm), as well as soil organic C, available N, and available P...
October 18, 2017: Canadian Journal of Microbiology
James Gillis, Kenton Ko, Juliana A Ramsay, Bruce A Ramsay
Greater than 65 % of canola and high oleic soy oil fatty acids is oleic acid, which is readily converted to nonanoic (NA) and azelaic (AzA) acids by ozonolysis. NA is an excellent substrate for medium-chain-length polyhydroxyalkanoate (mcl-PHA) production but AzA has few uses. Pseudomonas citronellolis DSM 50332 and Pseudomonas fluorescens ATCC 17400, both able to produce mcl-PHA from fatty acids and able to grow on AzA as the sole source of carbon and energy were assessed for the accumulation of mcl-PHA from AzA and NA...
October 17, 2017: Canadian Journal of Microbiology
Supansa Bunpa, Nishibuchi Mitsuaki, Jumroensri Thawonsuwan, Natthawan Sermwittayawong
<i>Vibrio alginolyticus</i> is ubiquitous in marine environments and is among the human and marine animal pathogens. The aims of this study were to analyze the relationships between genetic diversity and origins, and to develop new primers based on <i>gyrB</i> sequence for identification of <i>V. alginolyticus</i> isolated from various sources. To determine the genetic diversity of this bacterium, arbitrarily primed polymerase chain reaction (AP-PCR) technique was performed on 36 strains of <i>V...
October 10, 2017: Canadian Journal of Microbiology
Deborah A Court, Shivani Khetoo, Sabbir Rahman Shuvo, Shayne D Reitmeier, Georg Hausner
In eukaryotic cells communication and dynamic interactions among different organelles are important for maintaining cellular homeostasis. The Endoplasmic-Reticulum (ER) Mitochondria Encounter Structure (ERMES) complex establishes membrane contact sites between ER and mitochondria and is essential for phospholipid transport, protein import and mitochondrial dynamics and inheritance. In this work, in-silico analyses were used to probe the intramolecular interactions in ERMES proteins and the interactions that support the ERMES complex...
October 6, 2017: Canadian Journal of Microbiology
Parveen Kumar Sharma, Rifat Munir, Teresa de Kievit, David B Levin
Pseudomonas chlororaphis strain PA23 was isolated from soybean roots as a plant growth-promoting rhizobacterium (PGPR) and secretes a wide-range of compounds, including the antibiotics phenazine-1-caroxymide (PCA), pyrrolnitrin, and 2-hydroxyphenazine. We have determined that P. chlororaphis PA23 can synthesize medium chain length polyhydroxyalkanoate (PHA) polymers utilizing free fatty acids, such as octanoic acid and nonanoic acid, as well as vegetable oils as sole carbon sources. Genome analysis identified a pha operon containing 7 genes in P...
October 5, 2017: Canadian Journal of Microbiology
Jordyn Bergsveinson, Barry Ziola
Beer-spoilage-related lactic acid bacteria (BSR LAB) belong to multiple genera and species; however, beer-spoilage capacity is isolate-specific and partially acquired via horizontal gene transfer within the brewing environment. Thus, the extent to which genus-, species- or environment- (i.e., brewery-) level genetic variability influences beer-spoilage phenotype is unknown. Publicly available Lactobacillus brevis genomes were analyzed via BlAst Diagnostic Gene findEr (BADGE) for BSR genes and assessed for pangenomic relationships...
October 4, 2017: Canadian Journal of Microbiology
Wenguang Fan, Haiwei Ren, Yingying Cao, Yonggang Wang, Guicheng Huo
The aim of this study was to investigate the effects of a low dietary protein and high carbohydrate infant formula on the large intestine of neonatal rats. A total of 24 neonatal SD rats (14d old) were randomly assigned to the low-protein, high-carbohydrate infant formula-fed group (IF group) and a human breast milk-fed group (HF group). After 7 days, we selected 6 rats at random from each group to study. No significantly different microbial colonization patterns were observed in the two groups at the phylum level...
September 21, 2017: Canadian Journal of Microbiology
Marek Bartoszewicz, Urszula Czyzewska
Gram-positive rods named <i>B. cereus sensu lato</i> (<i>sl</i>), are common in natural habitats and food products. It is believed that they are restricted to spores, however their ecology in aquatic habitats is still poorly investigated. Thus, the aim of the study was to assess the rain-dependent fluctuations of the concentration of <i>B. cereus sl</i> vegetative cells and spores with evaluation of their phylogenetic and population structure in relation to the toxicity and psychrotolerance...
September 20, 2017: Canadian Journal of Microbiology
Kun Luo, Caro-Lyne DesRoches, Anne Johnston, Linda J Harris, Hui-Yan Zhao, Thérèse Ouellet
<i>Fusarium graminearum </i>is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight (FHB) in temperate regions of the world. Previous studies have shown that <i>F. graminearum </i>can synthetize indole-3-acetic acid (auxin) using L-tryptophan (L-TRP)-dependent pathways. In the present study, we have taken a broader approach to examine the metabolism of L-TRP in <i>F. graminearum </i>liquid culture. Our results showed that <i>F. graminearum </i>was able to transiently produce the indole tryptophol when supplied with L-TRP...
September 19, 2017: Canadian Journal of Microbiology
Manu Singh, Yvonne Yau, Shirley Wang, Valerie Waters, Ayush Kumar
In this study, we analyzed 15 multidrug resistant cystic fibrosis isolates of Pseudomonas aeruginosa from chronic lung infections for expression of four different multidrug efflux systems, MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY, using quantitative reverse transcriptase-PCR. Overexpression of MexXY pump was observed in all of the isolates tested. Analysis of regulatory genes that control the expression of these four efflux pumps revealed a number of previously uncharacterized mutations. Our work shows that MexXY pump overexpression is common in cystic fibrosis isolates and could be contributing to their reduced aminoglycoside susceptibility...
September 18, 2017: Canadian Journal of Microbiology
Chao Yang, Rosalind Bueckert, Jeff Schoenau, Axel Diederichsen, Hossein Zakeri, Thomas Warkentin
Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate super-nodulating pea mutant Rondo-nod3(fix+), hyper-nodulating pea mutant Frisson P88 Sym29, CDC Meadow commercial control, and a non-nodulating mutant Frisson P56 (nod-) to evaluate BNF in a greenhouse assay. Significant differences in strain×cultivar interactions were detected for shoot and root dry weight which ranged from 1...
September 18, 2017: Canadian Journal of Microbiology
Hui Xia, Qiongwei Tang, Jie Song, Jiang Ye, Haizhen Wu, Huizhan Zhang
Small colony variants (SCVs) are a commonly observed subpopulation of bacteria that have a small colony size and distinctive biochemical characteristics. SCVs are more resistant to some antibiotics than the wild-type, and usually cause persistent infections in the clinic. SCV studies have been very active during the past two decades, especially of <i>Staphylococcus aureus</i>. However, fewer studies on <i>Escherichia coli </i>SCVs exist, so we studied an <i>E. coli </i>SCV during an experiment involving the deletion of the<i> yigP</i> locus...
September 15, 2017: Canadian Journal of Microbiology
Xiangyun Zang, Meiting Liu, Han Wang, Yihong Fan, Haichang Zhang, Jiawen Liu, Enlu Xing, Xiuhong Xu, Hongtao Li
Cellulose degradation is an important part of global carbon cycle in composting process, and β-Glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. This work analyzes the succession of β-glucosidase-producing microbial communities that persists throughout composting, evaluates their metabolic activities and community advantage during the various phases of composting. Fungal and bacterial β-glucosidase genes belonging to glycoside hydrolase families GH1 and GH3 amplified from DNA and gene abundance levels were analyzed...
September 11, 2017: Canadian Journal of Microbiology
Dianne K Newman
September 7, 2017: Canadian Journal of Microbiology
Ayman El-Shibiny, Salma El-Sahhar
Since their discovery in 1915, bacteriophages have been used to treat bacterial infections in animals and humans because of their unique ability to infect their specific bacterial hosts without affecting other bacterial populations. The research carried out in this field throughout the 20th century, largely in Georgia, part of USSR and Poland, led to the establishment of phage therapy protocols. However, the discovery of penicillin and sulfonamide antibiotics in the Western World during the 1930s was a setback in the advancement of phage therapy...
September 1, 2017: Canadian Journal of Microbiology
Rui Han, Xin Zhang, Jing Liu, Qifu Long, Laisheng Chen, Deli Liu, Derui Zhu
Keke Salt Lake is located in the Qaidamu Basin of China. It is a unique magnesium sulfate-subtype hypersaline lake that exhibits a halite domain ecosystem, yet its microbial diversity has remained unstudied. Here, the microbial community structure and diversity was investigated via high-throughput sequencing of the V3-V5 regions of 16S rRNA genes. A high diversity of operational taxonomic units was detected for Bacteria and Archaea (734 and 747, respectively), comprising 21 phyla, 43 classes, and 201 genera of Bacteria and 4 phyla, 4 classes, and 39 genera of Archaea...
August 29, 2017: Canadian Journal of Microbiology
Ryan M Chanyi
No abstract is required for this submission.
August 25, 2017: Canadian Journal of Microbiology
Amélia Bourceret, Corinne Leyval, François Thomas, Aurélie Cébron
At centimeter scale, soil bacterial assemblages are shaped by both abiotic (edaphic characteristics and pollutants) and biotic parameters. In a planted industrial soil, contaminated with polycyclic aromatic hydrocarbons (PAHs), pollution was previously shown to be distributed randomly with hot and cold spots, using rhizoboxes. We therefore investigated the effect of this patchy PAH distribution on the bacterial community assemblage, and compared it to that of root depth gradients found in the rhizosphere of either alfalfa or ryegrass...
August 25, 2017: Canadian Journal of Microbiology
Wenxi Qian, ZhiPeng Li, Weiping Ao, Guangyong Zhao, JianPing Wu, Guangyu Li
No abstract text is available yet for this article.
October 2017: Canadian Journal of Microbiology
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