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Canadian Journal of Microbiology

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https://www.readbyqxmd.com/read/27990842/note-of-appreciation-note-de-reconnaissance
#1
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27958773/studies-on-the-biofilm-produced-by-pseudomonas-aeruginosa-grown-in-different-metal-fatty-acid-salt-media-and-its-application-in-biodegradation-of-fatty-acids-and-bioremediation-of-heavy-metal-ions
#2
P Abinaya Sindu, Pennathur Gautam
Metal fatty acid salts (MFAS) in untreated industrial effluents cause environmental pollution. The use of biocompatible agents for remediation may help in reducing the harm caused to the ambient aquatic organism. Pseudomonas aeruginosa is a ubiquitous organism that thrives under harsh conditions and is resistant to toxic metal ions. The present study shows a proof-of-concept of using this organism in the biodegradation of MFAS. MFAS were prepared and we studied their effect on the growth of the planktonic form and the formation of biofilm by P...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27919161/effect-of-rotor-type-on-the-separation-of-isotope-labeled-and-unlabeled-escherichia-coli-rna-by-isopycnic-density-ultracentrifugation
#3
Elena Herrmann, Patrick Koch, Christian U Riedel, Wayne Young, Markus Egert
Separation of differentially isotope-labeled bacterial RNA by isopycnic density gradient centrifugation is a critical step in RNA-based stable isotope probing analyses, which help to link the structure and function of complex microbial communities. Using isotope-labeled Escherichia coli RNA, we showed that an 8 mL near-vertical rotor performed better than a 2 mL fixed-angle rotor, thereby corroborating current recommendations. Neither increased concentrations of formamide nor urea in the medium improved the separation results using the fixed-angle rotor...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27912317/production-of-bacillus-amyloliquefaciens-og-and-its-metabolites-in-renewable-media-valorisation-for-biodiesel-production-and-p-xylene-decontamination
#4
Augusto Etchegaray, François Coutte, Gabrielle Chataigné, Max Béchet, Ramon H Z Dos Santos, Valérie Leclère, Philippe Jacques
Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27905820/evidence-based-validation-of-quorum-quenching-from-lysinibacillus-sphaericus-and-geobacillus-sp-in-bioremediation-of-oil-sludge
#5
Camilo Gómez-Garzón, Jenny Dussán
Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consortia in applications such as bioremediation. In the current work, we demonstrated the presence of a potential novel AHL-lactonase-encoding locus (Bsph_3377) from Lysinibacillus sphaericus and Geobacillus sp...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27900876/effects-of-production-methods-and-protective-ingredients-on-the-viability-of-probiotic-lactobacillus-rhamnosus-r0011-in-air-dried-alginate-beads
#6
Claude P Champagne, Yves Raymond, Yves Arcand
The goal of this study was to use a microencapsulation technology to prepare air-dried concentrated cultures of Lactobacillus rhamnosus R0011. The cultures were microencapsulated in alginate beads, which were added to a growth medium to allow cell multiplication inside the matrix; the beads were recovered, dipped in protective solutions, and air-dried. The effects of fermentation technology and of the composition of the protective solutions on subsequent survival during air-drying were examined. The cells prepared under a constant pH of 6...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27841947/selenium-uptake-and-assessment-of-the-biochemical-changes-in-arthrospira-spirulina-platensis-biomass-during-the-synthesis-of-selenium-nanoparticles
#7
I Zinicovscaia, T Chiriac, L Cepoi, L Rudi, O Culicov, M Frontasyeva, V Rudic
The process of selenium uptake by biomass of the cyanobacterium Arthrospira (Spirulina) platensis was investigated by neutron activation analysis at different selenium concentrations in solution and at different contact times. Experimental data showed good fit with the Freundlich adsorption isotherm model, with a regression coefficient value of 0.99. In terms of absorption dependence on time, the maximal selenium content was adsorbed in the first 5 min of interaction without significant further changes. It was also found that A...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27835736/citeromyces-matritensis-m37-is-a-salt-tolerant-yeast-that-produces-ethanol-from-salted-algae
#8
Masahiko Okai, Ayako Betsuno, Ayaka Shirao, Nobuo Obara, Kotaro Suzuki, Toshinori Takei, Masachika Takashio, Masami Ishida, Naoto Urano
Algae are referred to as a third-generation biomass for ethanol production. However, salinity treatment is a problem that needs to be solved, because algal hydrolysates often contain high salt. Here, we isolated the salt-tolerant ethanol-producing yeast Citeromyces matritensis M37 from the east coast of Miura Peninsula in Japan. This yeast grew under osmotic stress conditions (20% NaCl or 60% glucose). It produced 6.55 g/L ethanol from YPD medium containing 15% NaCl after 48 h, and the ethanol accumulation was observed even at 20% NaCl...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27819481/characterization-of-klebsiella-sp-strain-s1-a-bacterial-producer-of-secoisolariciresinol-through-biotransformation
#9
Yu-Jie Zhou, Songling Zhu, Dong-Hui Yang, Dan-Dan Zhao, Jia-Jing Li, Shu-Lin Liu
Secoisolariciresinol (SECO) is a lignan of potential therapeutic value for diseases such as cancer, but its use has been limited by the lack of ideal production methods, even though its precursors are abundant in plants, such as flaxseeds. Here, we report the characterization of a bacterial strain, S1, isolated from the human intestinal flora, which could produce secoisolariciresinol by biotransformation of precursors in defatted flaxseeds. This bacterium was a Gram-negative and facultatively anaerobic straight rod without capsules...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27819479/diversity-of-formyltetrahydrofolate-synthetase-genes-in-the-rumens-of-roe-deer-capreolus-pygargus-and-sika-deer-cervus-nippon-fed-different-diets
#10
Zhipeng Li, Gemma Henderson, Yahan Yang, Guangyu Li
Reductive acetogenesis by homoacetogens represents an alternative pathway to methanogenesis to remove metabolic hydrogen during rumen fermentation. In this study, we investigated the occurrence of homoacetogen in the rumens of pasture-fed roe deer (Capreolus pygargus) and sika deer (Cervus nippon) fed either oak-leaf-based (tannin-rich, 100 mg/kg dried matter), corn-stover-based, or corn-silage-based diets, by using formyltetrahydrofolate synthetase (FTHFS) gene sequences as a marker. The diversity and richness of FTHFS sequences was lowest in animals fed oak leaf, indicating that tannin-containing plants may affect rumen homoacetogen diversity...
January 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27848273/announcement-communiqu%C3%A3
#11
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27829287/the-influence-of-microbial-based-inoculants-on-n2o-emissions-from-soil-planted-with-corn-zea-mays-l-under-greenhouse-conditions-with-different-nitrogen-fertilizer-regimens
#12
Pamela Calvo, Dexter B Watts, Joseph W Kloepper, H Allen Torbert
Nitrous oxide (N2O) emissions are increasing at an unprecedented rate owing to the increased use of nitrogen (N) fertilizers. Thus, new innovative management tools are needed to reduce emissions. One potential approach is the use of microbial inoculants in agricultural production. In a previous incubation study, we observed reductions in N2O emissions when microbial-based inoculants were added to soil (no plants present) with N fertilizers under laboratory incubations. This present study evaluated the effects of microbial-based inoculants on N2O and carbon dioxide (CO2) emissions when applied to soil planted with corn (Zea mays L...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27822959/seasonal-changes-in-bacterial-communities-associated-with-healthy-and-diseased-porites-coral-in-southern-taiwan
#13
Chorng-Horng Lin, Chih-Hsiang Chuang, Wen-Hung Twan, Shu-Fen Chiou, Tit-Yee Wong, Jong-Kang Liu, Chyuan-Yao Kao, Jimmy Kuo
We compared the bacterial communities associated with healthy scleractinian coral Porites sp. with those associated with coral infected with pink spot syndrome harvested during summer and winter from waters off the coast of southern Taiwan. Members of the bacterial community associated with the coral were characterized by means of denaturing gradient gel electrophoresis (DGGE) of a short region of the 16S rRNA gene and clone library analysis. Of 5 different areas of the 16S rRNA gene, we demonstrated that the V3 hypervariable region is most suited to represent the coral-associated bacterial community...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27805417/prevalence-of-cry2-type-genes-in-bacillus-thuringiensis-isolates-recovered-from-diverse-habitats-in-india-and-isolation-of-a-novel-cry2af2-gene-toxic-to-helicoverpa-armigera-cotton-boll-worm
#14
Jawahar Lal Katara, Sarvjeet Kaur, Gouthami Krishna Kumari, Nagendra Kumar Singh
Insecticidal cry and vip genes from Bacillus thuringiensis (Bt) have been used for control of lepidopteran insects in transgenic crops. However, novel genes are required for gene pyramiding to delay evolution of resistance to the currently deployed genes. Two PCR-based techniques were employed for screening of cry2-type genes in 129 Bt isolates from diverse habitats in India and 27 known Bt strains. cry2Ab-type genes were more prevalent than cry2Aa- and cry2Ac-type genes. Correlation between source of isolates and abundance of cry2-type genes was not observed...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27805413/in-situ-identification-and-quantification-of-protein-hydrolyzing-ruminal-bacteria-associated-with-the-digestion-of-barley-and-corn-grain
#15
Yun Xia, Yunhong Kong, Heping Huang, Hee Eun Yang, Robert Forster, Tim A McAllister
In this study, BODIPY FL DQ™ casein staining combined with fluorescence in situ hybridization (FISH) was used to detect and identify protein-hydrolyzing bacteria within biofilms that produced active cell-surface-associated serine- and metallo-proteases during the ruminal digestion of barley and corn grain in cows fed barley-based diets at 2 different levels. A doublet coccoid bacterial morphotype associated with barley and corn grain particles fluoresced after BODIPY FL DQ™ casein staining. Bacteria with this morphotype accounted for 3%-10% of the total bacteria attached to surface of cereal grain particles, possibly indicative of an important role in the hydrolysis of the protein matrix within the endosperm...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27784163/variability-in-colonization-of-arbuscular-mycorrhizal-fungi-and-its-effect-on-mycorrhizal-dependency-of-improved-and-unimproved-soybean-cultivars
#16
M S Salloum, M C Guzzo, M S Velazquez, M B Sagadin, C M Luna
Breeding selection of germplasm under fertilized conditions may reduce the frequency of genes that promote mycorrhizal associations. This study was developed to compare variability in mycorrhizal colonization and its effect on mycorrhizal dependency (MD) in improved soybean genotypes (I-1 and I-2) with differential tolerance to drought stress, and in unimproved soybean genotypes (UI-3 and UI-4). As inoculum, a mixed native arbuscular mycorrhizal fungi (AMF) was isolated from soybean roots, showing spores mostly of the species Funneliformis mosseae...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27781484/molecular-characterization-of-pathogenic-and-nonpathogenic-fowl-aviadenovirus-serotype-11-isolates
#17
Patrick D Slaine, James G Ackford, Andrew M Kropinski, Robert A Kozak, Peter J Krell, Éva Nagy
Fowl aviadenoviruses, many of which are of importance in veterinary medicine, are classified into 5 species. In this study, a pathogenic isolate and a nonpathogenic isolate of fowl aviadenovirus serotype 11 (FAdV-11) of species Fowl aviadenovirus D were characterized. Growth rates were analyzed for the 2 isolates, showing notable differences. The complete genomic sequences of the viruses were fully determined and were analyzed. The genomes of the 2 isolates showed 98.1% sequence identity and revealed 6 nonsynonymous mutations between the Ontario isolates...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27763775/reliability-of-haemophilus-influenzae-biofilm-measurement-via-static-method-and-determinants-of-in-vitro-biofilm-production
#18
Najla A Obaid, Stephen Tristram, Christian K Narkowicz, Glenn A Jacobson
Information is lacking regarding the precision of microtitre plate (MTP) assays used to measure biofilm. This study investigated the precision of an MTP assay to measure biofilm production by nontypeable Haemophilus influenzae (NTHi) and the effects of frozen storage and inoculation technique on biofilm production. The density of bacterial final growth was determined by absorbance after 18-20 h incubation, and biofilm production was then measured by absorbance after crystal violet staining. Biofilm formation was categorised as high and low for each strain...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27759425/investigating-the-ability-of-pseudomonas-fluorescens-uw4-to-reduce-cadmium-stress-in-lactuca-sativa-via-an-intervention-in-the-ethylene-biosynthetic-pathway
#19
Lucas J Albano, Sheila M Macfie
A typical plant response to any biotic or abiotic stress, including cadmium (Cd), involves increased ethylene synthesis, which causes senescence of the affected plant part. Stressed plants can experience reduced ethylene and improved growth if they are inoculated with bacteria that have the enzyme ACC deaminase, which metabolizes the ethylene precursor ACC (1-aminocyclopropane-1-carboxylate). We investigated whether one such bacterium, Pseudomonas fluorescens UW4, reduces the production of ethylene and improves the growth of lettuce (Lactuca sativa) sown in Cd-contaminated potting material (PRO-MIX® BX)...
December 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27718589/increased-glucose-utilization-and-cell-growth-of-corynebacterium-glutamicum-by-modifying-the-glucose-specific-phosphotransferase-system-pts-glc-genes
#20
Jianzhong Xu, Junlan Zhang, Dongdong Liu, Weiguo Zhang
The phosphoenolpyruvate:glucose phosphotransferase system (PTS(Glc)) is the major pathway of glucose uptake in Corynebacterium glutamicum. This study investigated glucose consumption rate, cell growth, and metabolite changes resulting from modification of PTS(Glc). The classical l-lysine producer C. glutamicum XQ-8 exhibited low glucose consumption, cell growth, and l-lysine production rates, whereas these parameters were significantly increased during cultivating on glucose plus maltose, through inactivation of SugR, or by overexpression of PTS(Glc) genes...
December 2016: Canadian Journal of Microbiology
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