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Bulletin of Experimental Biology and Medicine

N P Makarova, Yu A Romanov, N V Dolgushina, M M Parker, A M Krasnyi
We analyzed the effect of cryopreservation on the expression of glutathione peroxidase (GPX1) and glutathione reductase (GSR) genes in human sperm cells (15 sperm samples from fertile donors and 10 samples from infertile patients). The relative expression of GPX1 and GSR genes was determined by real-time PCR. The rate of post-thaw recovery was 2.1 times higher in the group of fertile donors. A significant increase in the expression of GPX1, but not GSR, was observed in sperm samples from infertile patients, while in patients with infertility, GPX1 expression significantly decreased after cryopreservation/thawing, in samples from fertile donors after the same procedure it increased to the level observed in the sperm samples from infertile patients...
May 24, 2018: Bulletin of Experimental Biology and Medicine
L V Bayunova, I I Zorina, I O Zakharova, N F Avrova
We studied the protective effect of insulin in various concentrations and its effect on the Bax/ Bcl-2 ratio in neurons of rat cerebral cortex under conditions of oxidative stress. The protective effect of insulin was dose-dependent within the nanomolar range (1 nM<10 nM<100 nM). Preincubation with insulin in concentrations of 100 nM and 1 μM significantly increased Bcl-2 content in neurons in 5, 30, and 45 min and 1, 2, and 4 h after the start of cell exposure to H2 O2 . This prooxidant increased the Bax/Bcl-2 ratio in neurons to 141-164% in comparison with the control (100%); preincubation of neurons with insulin returned this ratio to normal...
May 24, 2018: Bulletin of Experimental Biology and Medicine
Yu V Nesterova, T N Povet'eva, N I Suslov, G N Zyuz'kov, V V Zhdanov, Yu S Fedorova, P V Kul'pin, K V Shaposhnikov
Repeated administration of songorine to mice restored mnestic processes impaired by scopolamine treatment, which manifested in improvement of CPAR conditioning and normalization of behavioral activity throughtout the observation period. This thearpeutical effect surpassed that of pyracetam used as the reference drug.
May 24, 2018: Bulletin of Experimental Biology and Medicine
O A Burmistrova, S V Nikulin, G S Zakharova, K A Fomicheva, B Ya Alekseev, M Yu Shkurnikov
During metastatic growth, cells of solid tumors undergo phenotypical changes related to epithelial-mesenchymal transition. Epithelial-mesenchymal transition is regarded as a potential target for prospective antitumor drugs. Fluorescent reporter systems for evaluation of the expression of markers of epithelial and mesenchymal status (E- and N-cadherins) were created. The described approaches can be used for creation of analogous reporter systems.
May 24, 2018: Bulletin of Experimental Biology and Medicine
N V Lyubimova, Yu S Timofeev, V M Abaev, O M Votyakova, N E Kushlinskii
The diagnostic potentialities of complex immunochemical analysis of the serum and daily urine were evaluated in 118 patients with multiple myeloma. In 95 patients, we observed secretion of monoclonal intact immunoglobulins with heavy chains G (N=69), A (N=19), and M (N=4) and biclonal secretion of paraproteins G and A (N=3). Bence-Jones protein was detected in the sera and daily urine of 16 patients and Bence-Jones proteinuria alone was detected in 3 patients. The diagnostic sensitivity of serum immunoelectrophoresis in multiple myeloma is 94...
May 24, 2018: Bulletin of Experimental Biology and Medicine
V G Motin, A V Kiselev, I S Stovbun, V I Sergienko, T S Kalinina
The study examined the effect of calcium salt of N-(5-hydroxynicotinoil)-L-glutamic acid (Ampasse preparation) on neuronal activity in hippocampal CA1 area evoked by stimulation of Schaffer collaterals at a rate of 1 Hz (30 impulses during 30 sec) in the surviving hippocampal slices of Wistar rats. The records of 1st and 30th orthodromic population spikes showed that Ampasse in concentrations of 500 μM, 1, 2, and 10 mM facilitated the synaptic transmission in Schaffer collaterals - hippocampal CA1 pyramidal neurons axis; the maximum effect was observed at 2 mM Ampasse...
May 24, 2018: Bulletin of Experimental Biology and Medicine
G V Zhukova, A I Schikhlyarova, T A Barteneva, A N Shevchenko, F M Zakharyuta
Pronounced antitumor effect of Thymalin in doses lower than the therapeutic doses was shown in experiments on albino outbred male rats with transplanted sarcoma 45. Tumor growth arrest and its regression were observed in more than half of animals and in other cases, the growth was suppressed by 78%. Microstructural changes in the thymus were analyzed. Significant increase in lymphoproliferative activity and the content of tissue basophils and plasmocytes in the thymus lobules was observed. Tumor regression was accompanied by the development of stable antistress adaptation reactions of calm and elevated activation...
May 24, 2018: Bulletin of Experimental Biology and Medicine
Yu S Kisrieva, N A Petushkova, N F Samenkova, G P Kuznetsova, O V Larina, N B Teryaeva, V G Zgoda, I I Karuzina, D U Usachev, A Yu Belyaev
Blood plasma proteome in patients with cerebral ischemia and healthy individuals was studied using comparative proteomic analysis based on tandem HPLC-MS/MS. Mass spectra were analysed in an automated mode using Progenesis LS-MS software and 256 proteins were identified. Significant quantitative differences were revealed for 20 proteins. It was found that changes in the blood plasma proteome in subjects with cerebral ischemia involved a wide range of proteins: molecular chaperones, fibrinolysis, angiogenesis, and immune system proteins, proteins involved in homeostasis maintenance, cell differentiation and proliferation, regulators of apoptosis, and cytoskeleton proteins...
May 24, 2018: Bulletin of Experimental Biology and Medicine
G N Zyuz'kov, E V Udut, L A Miroshnichenko, T Yu Polyakova, E V Simanina, L A Stavrova, G A Prosekin, M Yu Minakova, E V Borodulina, I V Mareev, R V Gurto, V V Zhdanov, V V Udut
JAK/STAT signaling pathway was examined during functional stimulation of mesenchymal progenitor cells with fibroblast growth factor. The differences were observed in the realizations of the proliferation-differentiation potential of CFU-fibroblasts under blockade of JAKs or during selective inactivation of STAT3. The study revealed stimulating influences of JAKs and STAT3 on mitotic activity of progenitor cells and individual roles of these proteins in the control of their maturation. Blockade of JAKs diminished the level of fibroblast colony formation and the score of actively proliferating CFU-fibroblasts at the background increase of the differentiation rate of progenitor cells...
May 24, 2018: Bulletin of Experimental Biology and Medicine
O V Varlamova, A V Babkin, I S Berdinskikh, G V Nazarov, A S Nikiforov, V N Bykov, M B Ivanov
We studied association of Oprm1 gene polymorphisms with signs of N-(1-phenethyl-4-piperidyl)propionanilide intoxication in rats. It was found that the rate of intoxication in laboratory animals depends on genetic features. A polymorphic variant rs105312806 of Oprm1 gene can be a possible marker of animal sensitivity to opioid receptor agonists. This hypothesis was supported by differences in the rats of intoxication signs such as time to lateral posture and sleep duration in homozygous rats carrying different alleles...
May 24, 2018: Bulletin of Experimental Biology and Medicine
A A Kechin, U A Boyarskikh, N A Ermolenko, A S Tyulyandina, D G Lazareva, A M Avdalyan, S A Tyulyandin, N E Kushlinskii, M L Filipenko
Changes (or variants) in BRCA1 and BRCA2 gene sequences can have different lengths and clinical significance: from single nucleotide variants (SNV) and short insertions/deletions (<50 bp) to extended deletions and duplications (so-called copy number variations, or CNV). According to their clinical significance, all variants can be divided into pathogenic, likely pathogenic, variants of uncertain significance, likely benign, and benign. Moreover, variants can be germinal (i.e. inherited from parents) and somatic (arising in the process of development of the organism)...
May 24, 2018: Bulletin of Experimental Biology and Medicine
V Z Agrba, D D Karal-Ogly, T E Gvozdik, P A Kaplanyan, N P Demenkova, I A Gvaramiya, E I Mukhametzyanova, O A Shamsutdinova, Yu P Chuguev, S Sh Kal'sina, A G Konoplyannikov, D E Araviashvili, I N Klots, B A Lapin
Three injections of doxorubicin to rhesus macaques cause severe intoxication, characterized by anemia, cachexia, and degeneration of the viscera. The life span of monkeys injected with the drug and receiving after 24 h mesenchymal stem cell transplantation varied from 96 to 120 days in comparison with 50-74 days in animals receiving stem cells before doxorubicin. Controls received doxorubicin and saline; the lifespan of one monkey was 24 days, of the other - 1 year and 8 months. The increase in activity of proinflammatory cytokine IL-6 was paralleled by an increase in the level of C-reactive protein...
May 24, 2018: Bulletin of Experimental Biology and Medicine
N V Krylova, G N Leonova, O S Maystrovskaya, A M Popov, A A Artyukov
Antiviral activity of the polyphenol complex from seagrass of the Zosteraceae family against highly pathogenic strain of the tick-borne encephalitis virus was studied on passaged culture of porcine embryo kidney cells. The antiviral effect of the test compound manifested in a decrease in the infectious titer of the virus and depended on the concentration and application schemes. Polyphenol complex in a concentration of 100 μg/ml suppressed accumulation of the pathogen in the cell culture: pretreatment of the virus reduced its titer by 4 log, pretreatment of cells by 1...
May 24, 2018: Bulletin of Experimental Biology and Medicine
S A Apryatin, V A Shipelin, Yu S Sidorova, N A Petrov, I V Gmoshinskii, D B Nikityuk
We assessed the effect of intake of easily digested carbohydrates for 133 days on quantitative parameters of neuromotorics and cognitive function in Wistar rats and C57Bl/6J mice. Neuromotorics (muscle tone) was assessed in rats and mice by the forelimb muscle force (grip strength) over 4 months. Anxiety was assessed in the elevated plus-maze test and cognitive function (short-term and long-term memory) was evaluated by conditioned passive avoidance response (CPAR) test over 3 months. The mice, in contrast to rats, receiving the diet with easily digested sugars demonstrated suppression of neuromotorics...
May 24, 2018: Bulletin of Experimental Biology and Medicine
L V Topchieva, I V Kurbatova, O P Dudanova, A A Sokolovskaya, A A Shipovskaya
Association of IL6R gene polymorphic variant rs2228145(C>A) with the development of nonalcoholic steatohepatitis in Karelia residents is detected. The risk of nonalcoholic steatohepatitis is more than 2-fold higher in carriers of CC genotype by rs2228145 polymorphic marker than in carriers of other genotypes. Plasma levels of IL-6 and the content of IL6R gene transcripts in the peripheral blood leukocytes are higher in patients with nonalcoholic steatohepatitis than in normal subjects. No relationships between rs2228145 polymorphism and the level of IL-6 and content of IL6 and IL6R mRNA were detected...
May 24, 2018: Bulletin of Experimental Biology and Medicine
A V Smirnov, D S Mednikov, M V Shmidt, D V Kurkin, D A Bakulin, E V Volotova, I N Tyurenkov
Structural changes in the rat hippocampus in response to chronic cerebrovascular disorders induced by gravity exposure in the caudocranial vector were studied. Qualitative and quantitative morphological analysis detected significant cytoarchitectonic changes in the pyramidal layer: spongiosis, manifest pericellular and perivascular edema, and a drastic increase in the counts of pyramidal neurons with signs of impairment in all hippocampal zones. The density of perikarya in the pyramidal layer decreased. Immunohistochemical study detected high expression of Beclin-1 in CA1 field...
May 24, 2018: Bulletin of Experimental Biology and Medicine
O V Povarova, A V Balatsky, V S Gusakov, O S Medvedev
The study examined the effect of endogenous lipid-soluble antioxidant coenzyme Q10 on the expression of UbiA gene of prenyltransferase domain-containing protein 1 (UbiAd1) involved in synthesis of vitamin K2 (and probably of coenzyme Q10) on a rat model of ischemic stroke provoked by ligation of the middle cerebral artery in the left hemisphere. Ischemia enhanced expression of mRNA of UbiAd1 gene in both cerebral hemispheres, but the effect was significant only in the contralateral one. The study revealed no effect of intraperitoneal injection of coenzyme Q10 (30 mg/kg) on ischemia-produced elevation of mRNA of UbiAd1 gene...
May 24, 2018: Bulletin of Experimental Biology and Medicine
T A Danilova, T A Smirnova, G A Danilina, A A Adzhieva, S G Andreevskaya, N V Shevlyagina, V G Zhukhovitsky
Our study confirmed the capacity of S. pyogenes strains to form biofilms on abiotic surfaces. Chains of streptococci surrounded by bluish film were seen under a microscope after alcian blue staining of the preparations grown on slides. On ultrathin sections in transmission electron microscope, the extracellular matrix (indicator of biofilm maturity) became visible after staining with alcian blue. Microscopy of the sections shows structures characteristic of a biofilm in spaces between the cells. Scanning electron microscopy also demonstrates the presence of a biomembrane...
May 24, 2018: Bulletin of Experimental Biology and Medicine
V Z Agrba, V Z Agrba, D D Karal-Ogly, S Sh Kal'sina, A G Konoplyannikov, T E Gvozdik, I A Gvaramiya, I N Klots, E I Mukhametzyanova, Yu P Chuguev, O A Shamsutdinova, D E Araviashvili, V A Porkhanov, B A Lapin
We studied physiological parameters of rhesus monkeys after administration of anthracycline antibiotic doxorubicin. Intravenous administration of the drug caused intoxication manifested in in an abrupt body weight loss, baldness, vomiting, and exicosis. Intoxication in monkeys determined by the damaging effects of doxorubicin on organs and tissues is also characterized by significant changes in the blood: leukopenia, thrombocytopenia, neutropenia, monocytopenia, lymphocytosis, and a sharp drop of CD20+ B cell content...
May 24, 2018: Bulletin of Experimental Biology and Medicine
K N Bardakova, E A Grebenik, E V Istranova, L P Istranov, Yu V Gerasimov, A G Grosheva, T M Zharikova, N V Minaev, B S Shavkuta, D S Dudova, S V Kostyuk, N N Vorob'eva, V N Bagratashvili, P S Timashev, R K Chailakhyan
We created an anisotropic material based on collagen sponge and reactive polylactide structured by laser photopolymerization. The combination of collagen with reactive polylactide improves the resistance of the formed matrices to biodegradation in comparison with collagen sponge, while the existence of sites with different mechanical characteristics and cell affinity on the matrix provides directed cell growth during their culturing. It was shown that reinforcement of the collagen sponges 7-fold increased the mean Young's modulus for the hybrid matrix without affecting its cytotoxicity...
May 24, 2018: Bulletin of Experimental Biology and Medicine
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