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Bulletin of Experimental Biology and Medicine

M A Mukhamedyarov, P N Grigor'ev, E A Ushanova, T L Zefirov, A V Leushina, A L Zefirov
The function of synaptic transmission and presynaptic vesicular cycle in the neuromuscular synapses of the diaphragm was studied in transgenic APP/PS1 mice (Alzheimer's disease model). The decrease in the quantal content of end-plate potential, intense depression of the amplitude of terminal plate potentials under conditions of lasting high frequency stimulation (50 Hz), a drastic prolongation of the synaptic vesicle recycling time in APP/PS1 mice in comparison with wild type mice were detected. Manifest dysfunction of the neuromuscular synapses, caused by disordered neurosecretion and recycling of the synaptic vesicles in the presynaptic nerve endings, was detected in the Alzheimer's disease model on transgenic APP/PS1 mice...
September 2018: Bulletin of Experimental Biology and Medicine
N I Ziyatdinova, A M Kuptsova, L I Faskhutdinov, A L Zefirov, T L Zefirov
We studied the effect of α2 -adrenoreceptor agonist clonidine hydrochloride in concentrations of 10-9 -10-6 M on inotropy, chronotropy, and coronary flow in Langendorff-isolated heart of adult rats. It was found that α2 -adrenoreceptor agonist changed all studied parameters. Left ventricular myocardium contraction force decreased after application of all tested concentrations, the maximum effect was observed at a concentration of 10-6 M. Stimulation of α2 -adrenergic receptors in concentrations of 10-8 , 10-7 , and 10-6 M produced a two-phase effect (initial increase and a subsequent decrease) on the coronary flow...
September 2018: Bulletin of Experimental Biology and Medicine
M A Bakarev, V P Levin, I V Kachesov, N A Abdullaev, A I Neimark
Immunohistochemical and morphometric analysis of the microcirculatory bed in the tumor and non-tumor parenchyma of the prostate was carried out with the use of endothelial cell marker CD34 in patients treated by high-intensity focused ultrasound (HIFU). The numerical density of microvessels in the adenocarcinoma focus did not correlate with the degree of its differentiation, while high values of this parameter were associated with lower incidence of local progression after HIFU. Effective HIFU ablation led to progressive fibrosis and significant reduction of the microcirculatory bed in zones of intact non-tumor glands in control samples; an inverse relationship between the degree of reduction of the microcirculatory bed and the probability of relapse was revealed...
September 2018: Bulletin of Experimental Biology and Medicine
E V Kalinina, Y A Andreev, A S Petrova, K I Lubova, A A Shtil', N N Chernov, M D Novichkova, N K Nurmuradov
Expression of genes that plays a significant role in the control of cellular redox homeostasis was studied during the development of drug resistance of human ovarian adenocarcinoma SKOV-3 cells to cisplatin. It was found that the development of drug resistance was accompanied by enhanced expression of the genes encoding the key antioxidant enzymes (SOD2, CAT, GPX1, and HO-1) and transcription factor Nrf2, as well as reduced expression of the gene encoding NOX5 isoform of NADPH oxidase. The results testify to redox-dependent development of the adaptive antioxidant response as an important process in the mechanism of formation of resistance to cisplatin...
September 2018: Bulletin of Experimental Biology and Medicine
N A Kibler, V P Nuzhny, D N Shmakov
The contractile function of the heart was studied in adult frogs Rana temporaria under the influence of a toxic dose of isoprenaline under conditions of natural sinoatrial rhythm and during heart pacing. The dynamics of ventricular pressure was recorded with a Prucka MacLab 2000 instrument via a catheter introduced into the ventricle through the ventricular wall. Reduced (p<0.05) parameters of the pump function (HR, maximum ventricular systolic pressure, isovolumic indices dP/dtmax and dP/dtmin ) and lengthening of QRS complex and QT interval on ECG attested to impairment of contractile function and electrical processes after exposure to isoprenaline...
September 2018: Bulletin of Experimental Biology and Medicine
D S Ushakov, A S Dorozhkova, E V Babayants, V Yu Ovchinnikov, D N Kushlinskii, L V Adamyan, L F Gulyaeva, N E Kushlinskii
We studied microRNA whose expression can be regulated by carcinogenic compounds. Bioinformatic analysis has detected microRNA potentially regulated by xenosensor receptors AhR (miR-28, miR-30c, miR-30e, miR-139, and miR-153) and CAR (miR-29c, miR-31, miR-185, miR-625, and miR-652). Published data indicate that these microRNAs are oncosuppressors, except miR-31 that can act as an oncogene. The expression of these microRNAs in malignant tumors of the endometrium was studied. The expression of the majority of the studied microRNAs, except miR-652, was 2-3-fold below the normal, which confirms their oncosuppressor function and indicates their involvement in the endometrial carcinogenesis and hence, allows considering them as potential markers of the disease...
September 2018: Bulletin of Experimental Biology and Medicine
T M Plotnikova, G A Chernysheva, V A Smol'yakova, P P Shchetinin, A V Kuchin, I Yu Chukicheva, M B Plotnikov
We studied the cardioprotective effect of 2,6-diisobornyl-4-methylphenol under conditions of myocardial ischemia/reperfusion in rats. Daily administration of 2,6-diisobornyl-4-methylphenol (100 mg/kg intragastrically) over 3 days before and 5 days after modeling of myocardial ischemia/reperfusion prevented the increase in the infarction area by almost 2 times in comparison with the control by day 5 after recirculation. The type and severity of pathological changes in ECG parameters reflecting necrotic changes in the myocardium under the action of the compound significantly decreased by day 35 of the experiment...
September 2018: Bulletin of Experimental Biology and Medicine
I S Lebedeva, Ya R Panikratova, O Yu Sokolov, D A Kupriyanov, A D Rumshiskaya, N V Kost, N F Myasoedov
The effects of nootropic drug Semax on the neuronal network of the brain were studied by the resting state functional magnetic-resonance imaging (resting state fMRI). The study was carried out on two groups of healthy volunteers (11 men and 13 women aged 43.9±9.5 years). Resting state fMRI was carried out 3 times: directly before and 5 and 20 min after intranasal 1% Semax (14 subjects) or placebo (10 subjects). The topography of the resting state default mode network was studied. A greater volume of the default mode network rostral (medial frontal cortex) subcomponent was detected in the Semax group in comparison with controls...
September 2018: Bulletin of Experimental Biology and Medicine
E V Kur'yanova, A V Tryasuchev, V O Stupin, D L Teplyi
Single injection of muscarinic cholinoceptor blocker atropine (1 mg/kg) to outbred male rats reduced β-adrenergic responsiveness of erythrocytes (by 2.2 times) and the content of epinephrine granules on erythrocytes (by 1.5 times), significantly increased HR and rigidity of the heart rhythm, and manifold decreased the power of all spectral components of heart rhythm variability. Stimulation of the central neurotransmitter systems increased β-adrenergic responsiveness of erythrocytes (by 15-26%), decreased the number of epinephrine granules on erythrocytes (by 25-40%), and increased HR and cardiac rhythm intensity...
September 2018: Bulletin of Experimental Biology and Medicine
L А Sever'yanova, D V Plotnikov
We studied the effects of intraperitoneal administration of L-arginine in doses of 5, 15, and 50 μg/kg and peptides in doses containing equimolar amount of this amino acid on aggressive-defensive behavior of rats (footshock model). The peptides were synthesized by binding of Pro-Gly-Pro sequence to one or both ends of the L-arginine molecule. The analgesic and antiagressogenic effects of L-arginine and opposite effects of arginine-containing peptides (except Pro-Gly-Pro tripeptide) were demonstrated. The combination of arginine with glyprolines yielded peptides with intrinsic regulatory properties...
September 2018: Bulletin of Experimental Biology and Medicine
W I Kaganov
Some electrocardiograms, e.g. electrocardiograms recorded in patients with atrial fibrillation, myocardial infarction, or receiving some medications, contain waves of small amplitude. Despite low amplitude, these waves can significantly affect correct identification of the pathological process and diagnosis. The approach proposed by us allows studying these small waves using the wavelet analysis by constructing the corresponding wavelet image of ECG using special software. Two examples of construction of wavelet images of ECG in atrial fibrillation and myocardial infarction are presented...
September 2018: Bulletin of Experimental Biology and Medicine
T I Nikolaeva, K S Laurinavichus, V V Kaptsov, M V Molchanov, P V Shekhovtsov
Enzymatic hydrolysis of biopolymers of the cartilage tissue was studied for obtaining a complex of type II collagen peptides and glycosaminoglycan oligosaccharides. Hydrothermal hydrolysis in a high pressure homogenizer followed by enzymatic hydrolysis of the cartilage tissue biopolymers with proteolytic enzyme preparation Karipazim yielded a complex of collagen peptides and glycosaminoglycan oligosaccharides with molecular weights of 240-720 Da. Low molecular weight of the components increases their bioavailability...
September 2018: Bulletin of Experimental Biology and Medicine
M B Plotnikov, G A Chernysheva, V I Smol'yakova, T M Plotnikova, S V Sysolyatin, Y A Kryukov
We studied anti-ischemic activity of n-tyrozol under conditions of repeated transient myocardial ischemia in rats caused by repeated (5×3 min) occlusion of the left coronary artery. n-Tyrozol administered intraperitoneally in a dose of 20 mg/kg daily over 4 days before the ischemia modeling (the last injection 15 min prior to the start of the experiment) produced a clear-cut anti-ischemic effect: it reduced ST elevation and promoted more complete recovery of ECG during reperfusion. During reperfusion periods, n-tyrozol significantly decreased the risk of ventricular fibrillation and shortened the duration of tachyarrhythmia episodes (ventricular tachycardia and fibrillation)...
September 2018: Bulletin of Experimental Biology and Medicine
A A Zverev, T A Anikina, N G Iskakov, A L Zefirov, T L Zefirov
We studied the effect of neuropeptide Y in concentrations of 10-8 -10-6 M on electrical activity of adult rat right atrial cardiomyocytes with preserved spontaneous activity. Neuropeptide Y was found to modulate the amplitude-time parameters of action potential: in concentrations of 10-7 and 10-6 M it reduced the membrane potential, increased the amplitude of action potential and duration of the repolarization phase, and reduced the frequency of action potential generation. In concentration of 10-6 M, neuropeptide Y produced stronger effect on the analyzed parameters, while in concentration of 10-8 M it produced no significant changes...
September 2018: Bulletin of Experimental Biology and Medicine
L M Kozhevnikova, I B Tsorin, V N Stolyaruk, I F Sukhanova, M B Vititnova, T D Nikiforova, L G Kolik, S A Kryzhanovskii
The expression of Epac proteins (exchange protein directly activated by cAMP) and calmodulin (CaM) was assessed by the content of the corresponding mRNA in biopsy specimens of cardiac atrium, left ventricle, and thoracic aorta of rats with alcoholic cardiomyopathy. In the myocardium, overexpression of Еpac1, Ерас2, and СаМ mRNA was found. The content of Epac2 mRNA in the left ventricle was elevated by 2.9 times (p=0.000001), in the left atrium by 3.2 times (p=0.00001), in the right atrium by 3 times (p=0...
September 2018: Bulletin of Experimental Biology and Medicine
G I Lobov, D V Unt
LPS has an inhibitory effect on contractile activity of bovine mesenteric lymphatic vessels and nodes and causes a pronounced decrease in the tone and phase contractions. The selective inhibitor of inducible NO synthase, 1400W, and cyclooxygenase-2 selective inhibitor, dynastat, significantly attenuated the inhibitory effect of LPS. Dexamethasone interferes with the inhibitory effect of LPS on bovine lymphatic vessels and nodes. It was concluded that LPS stimulates expression of inducible NO synthase and cyclooxygenase-2 in endothelial and smooth muscle cells of lymphatic vessels and nodes...
September 2018: Bulletin of Experimental Biology and Medicine
A M Dolgorukova, V Yu Titov, V A Petrov, A N Osipov, N A Slesarenko, I I Kochish
The study of NO metabolism in chicken embryos showed that the intensity of oxidation of both endogenous and exogenous for the embryo NO donors to nitrate is determined by the presence or state of NO targets, rather than donor concentration. The mechanism of this oxidation and its physiological role are discussed. It was also shown that oxidation product nitrate is actively eliminated from the amnionic sac.
September 2018: Bulletin of Experimental Biology and Medicine
V I Konenkov, E G Koroleva, N B Orlov, V F Prokof'ev, A V Shevchenko, A M Novikov, T I Dergacheva
We analyzed cytokine profile in blood serum of patients with uterine myoma and revealed significantly reduced level of IFNγ and a tendency towards a decrease in the levels of IL-1β and TNFα; the levels of IL-6, IL-8, and IL-12p70 did not differ from those in healthy women. The drop in the concentrations of factors responsible for inflammation and angiogenesis in tissues are unfavorable for proliferation and differentiation of the uterine tissues.
September 2018: Bulletin of Experimental Biology and Medicine
L M Erofeeva, M V Mnikhovich
Changes in the structure and cell composition of carinal lymph nodes were studied in humans during aging. Replacement of node parenchyma with fibrous connective tissue progressing with age was demonstrated. The medullary matter significantly prevailed over the cortical substance. The lymph nodes in the cortical substance were small and had no light centers; the concentration of mature CD20+ B cells was high; the paracortical area was fragmented and thinned and contained no CD4+ T helpers. Ki-67+ cells were absent in all structural components of the lymph nodes reflecting exhaustion of lymphopoietic function, which was determined by the replacement of the reticular tissue of the microenvironment with the connective tissue and by the absence of CD4+ T cells regulating cellular and humoral immunity...
September 2018: Bulletin of Experimental Biology and Medicine
D A Korshunov, I A Klimov, V V Ivanov, I V Kondakova
Antitumor effects of glycolysis inhibitors monoiodoacetate and 2-deoxyglucose were studied on Lewis lung carcinoma model. Monoiodoacetate exhibited antitumor and antimetastatic activities, being not inferior of methotrexate (reference drug); however, the preparation also demonstrated high systemic toxicity. 2-Deoxyglucose exhibited only antitumor effect, while its antimetastatic activity did not differ from the result in the group without treatment.
September 2018: Bulletin of Experimental Biology and Medicine
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