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Bulletin of Experimental Biology and Medicine

V I Kaledin, O A Koval, E V Kuligina, E L Lushnikova, V P Nikolin, N A Popova, I A Pyshnaya, V A Richter
Antimetastatic effect of the liposomal form of recombinant lactaptin RL2 (a proteolytic fragment of human breast milk κ-casein; 8.6 kDa) was studied in A/Sn mice after intravenous transplantation of GA-1 tumor with high rate of liver metastases. Tumor growth in the liver was found in all mice. In animals dying early, the tumors were presented by multiple nodes of about the same size; in mice dying later, the tumors in the liver were presented by just few large nodes formed by cells that survived chemotherapy...
April 17, 2018: Bulletin of Experimental Biology and Medicine
E N Knyazev, D V Mal'tseva, A A Zakharyants, G S Zakharova, O V Zhidkova, A A Poloznikov
We studied expression profile of microRNA and their target genes in human umbilical vein endothelial cells (HUVEC) during proinflammatory activation with TNFα. TNFα-induced activation of HUVEC was accompanied by a decrease in the expression of CDKN1B, HIST1H3D, and OIP5 genes that are the common target genes for mature microRNA encoded by MIR221, MIR222, and MIR181B1 genes, whose expression increases in activated cells. Proteins encoded by HIST1H3D and OIP5 genes are associated with chromatin compaction and cell cycle...
April 17, 2018: Bulletin of Experimental Biology and Medicine
S V Revenko, L N Tikhomirova, A V Nesterov, I A Tarakanov
In experiments on narcotized rats, BP in the left femoral artery as well as local electrical potential and electrical impedance of the symmetric segment of the right femoral artery were simultaneously recorded in situ with two extracellular nonpolarizable Ag/AgCl electrodes located along the artery at a distance of 3 mm from each other. The pulsatile arterial electrical potentials with amplitude of 100-200 μV and duration of about 50 msec were recorded, which coincided with the front of BP wave corrected for a 10-msec delay of the pressure transducer...
April 16, 2018: Bulletin of Experimental Biology and Medicine
V P Demkin, V V Udut, P P Shchetinin, M V Svetlik, S V Mel'nichuk, A P Shchetinina, M O Pleshkov, D N Starkov, O V Demkin, H Kingma
We propose a new approach to optimization of electrical stimulation of the vestibular nerve and improving the transfer function of vestibular implant. A mathematical model of the vestibular organ is developed based on its anatomy, the model premises, 3D-analysis of MRI and CT images, and mathematical description of physical processes underlying propagation of alternating electric current across the tissues of vestibular labyrinth. This approach was tested in vitro on the rat vestibular apparatus and had been examined anatomically prior to the development of its mathematical model and equivalent electrical circuit...
April 16, 2018: Bulletin of Experimental Biology and Medicine
E V Udut, P P Shchetinin, T V Rudenko, F Lucieer, H Kingma, A P Shchetinina, M O Pleshkov, V P Demkin, V V Udut
Comparative analysis of the groups of patients with idiopathic bilateral vestibular hypofunction and a group of vestibulopathy patients with vertebrobasilar insufficiency demonstrated identity of the basic and additional diagnostic parameters in these syndromes as well as similarity in clinical diagnostic and anamnesis data. In all cases, functional assessment of endothelium-dependent vasodilation and selected biochemical marker sICAM-1 revealed endothelial dysfunction. Drug correction of endothelial dysfunction positively affected the manifestations of major and minor features of the syndrome, which confirmed the contribution of endothelial functional disturbances to the pathogenesis of bilateral vestibular hypofunction...
April 16, 2018: Bulletin of Experimental Biology and Medicine
A M Krasnyi, M I Gracheva, A A Sadekova, V V Vtorushina, I S Balashov, N E Kan, P I Borovikov, L V Krechetova, V L Tyutyunnik
We performed a complex analysis of total and fetal extracellular DNA, 8 cytokines (IL-2, IL-4, IL-6, IL-8, IL-10, granulocyte-macrophage CSF, IFNγ, and TNFα) in blood plasma obtained from women with preeclampsia prior to labor onset. Total (sensitivity 89.47%, specificity 93.75%) and fetal extracellular DNA (sensitivity 73.68%, specificity 87.5%) were the most accurate parameters determining preeclampsia. We revealed a high correlation (p=3×10-6 ) between total and fetal extracellular DNA levels in the group of preeclampsia...
April 16, 2018: Bulletin of Experimental Biology and Medicine
L I Kolesnikova, L V Rychkova, L R Kolesnikova, M A Darenskaya, L V Natyaganova, L A Grebenkina, L I Korytov, S I Kolesnikov
Mature normotensive male WAG rats and stress-sensitive hypertensive ISIAH rats were exposed to "everyday life stress" modelled by alternation of immobilization and adaptation. Increased LPO intensity (increased content of substrates with unsaturated double bonds and primary and secondary LPO products) and reduced content of some antioxidant protection components (reduced retinol level and GSH/GSSG ratio) were revealed in the blood of ISIAH rats. These changes correlated with elevated mean BP. The results can reflect the significant role of LPO changes in the pathogenesis of stress-induced arterial hypertension...
April 16, 2018: Bulletin of Experimental Biology and Medicine
M S Tverskaya, L V Gankovskaya, V V Sukhoparova, A O Virganskii
Effect of natural complex of cytokines with activity of IL-1, IL-2, IL-6, TNF, MIF, and GTFβ on the structure and metabolism of conduction cardiomyocytes was assessed in the control and under acute experimental aortic stenosis. After systemic administration of the cytokine complex in the control, structural abnormalities were revealed in a relatively low number of conduction cardiomyocytes; their relative number increased in the left ventricle and interventricular septum. When the complex was administered against the background of aortic stenosis, morphological changes in the conduction system were seen in a significant number of cells with their plasma imbibition, especially in the left ventricle and interventricular septum...
April 16, 2018: Bulletin of Experimental Biology and Medicine
E A Fedorova, T A Smolonogina, I N Isakova-Sivak, D A Koren'kov, T S Kotomina, G F Leont'eva, A N Suvorov, L G Rudenko
A project of an experimental recombinant vector vaccine for prevention of diseases caused by pathogenic streptococci based on ScaAB lipoprotein of Streptococcus agalactiae and a coldadapted strain of live influenza vaccine as a vector was developed. The sequence of ScaAB lipoprotein was analyzed and fragments forming immunodominant epitopes were determined. Chimeric molecules of influenza virus hemagglutinin H7 carrying insertions of bacterial origin were constructed. Based on the results of simulation, the most promising variants were selected; they represented fragments of lipoprotein ScaAB lacking N-terminal domain bound to hemagglutinin via a flexible linker...
April 16, 2018: Bulletin of Experimental Biology and Medicine
R U Ostrovskaya, S S Yagubova, T A Gudasheva, S B Seredenin
Reduced proliferation and enhanced apoptosis of β cells in diabetes mellitus are associated with a deficiency of brain-derived neurotrophic factor (BDNF). Low-molecular weight compounds similar to different BDNF loops were synthesized at the V. V. Zakusov Research Institute of Pharmacology. They produce a potentiating effect on TrkB phosphorylation, but differently activate post-receptor signaling pathways. We compared their effects on the severity of streptozotocin-induced diabetes mellitus in C57Bl/6 mice...
April 16, 2018: Bulletin of Experimental Biology and Medicine
A V Chechushkov, P M Kozhin, N S Zaitseva, P I Gainutdinov, E B Men'shchikova, A V Troitskii, V A Shkurupy
We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype)...
April 16, 2018: Bulletin of Experimental Biology and Medicine
O N Poteryaeva, G S Russkikh, A V Zubova, M M Gevorgyan, I F Usynin
In patients with type 2 diabetes mellitus, serum activities of MMP-2 and MMP-7 were substantially decreased in comparison with apparently healthy individuals. At the decompensation stage, along with the increased content of glucose and glycated hemoglobin, a pronounced (3-fold) increase in proinsulin concentration was observed. On the contrary, MMP activity and C-peptide concentration decreased at this stage. The ratio of proinsulin concentration to MMP activity at the stages of diabetes mellitus compensation and subcompensation was approximately 1:50, while at the stage of decompensation it was 1:12...
April 16, 2018: Bulletin of Experimental Biology and Medicine
N N Drozd, M A Torlopov, E V Udoratina, Yu S Logvinova
Nanocrystalline particles of chitin in the form of hydrosol in a concentration of 0.63 mg/ml have no effect on aggregation of human platelets and clotting time of platelet-poor plasma in coagulation tests. ADP-induced aggregation of human platelets was inhibited by these nanoparticles in concentrations of 0.63 and 1.00 mg/ml in comparison with the control. Intravenous administration of nanoparticles in a dose of 1 mg/kg to guinea pigs produced no anticoagulant effect. The nanocrystalline particles of chitin can be of interest as potential drug delivery systems...
April 16, 2018: Bulletin of Experimental Biology and Medicine
E N Knyazev, D V Mal'tseva, A A Zacharyants, G S Zakharova, O V Zhidkova, A A Poloznikov
Endothelial HUVEC cells used as an in vitro model of the endothelial monolayer in placental barrier were activated by TNFα in a dose of 2 ng/ml for 24 h. Significant changes in the expression of genes of the SLC family transport protein were observed: an increase in the expression of SLC7A2, SLC12A2, SLC9B2, SLC25A37, SLC16A9, and SLC41A2 and a decrease in the expression of SLC40A1. These transporters participate in the transport of iron, magnesium, sodium, potassium, and chloride ions, protons, and amino acids...
April 16, 2018: Bulletin of Experimental Biology and Medicine
O V Dolgikh, N V Zaitseva, A E Nosov, A V Krivtsov, D G Dianova, O A Kazakova, E A Otavina, I N Alikina
We studied the role of the carrier status for polymorphic loci of genes encoding estrogen receptors (ESR1), endothelial NO synthase (eNOS), and apolipoprotein E (APOE4) and products of their expression nitrogen oxide (NO) and apolipoprotein (ApoE) in the development of arterial hypertension in men. Conventionally healthy volunteers and 149 men with clinical manifestations of stage I-II arterial hypertension were examined. In men with arterial hypertension, the frequency of minor allele A of ESR1 gene was higher (27...
April 16, 2018: Bulletin of Experimental Biology and Medicine
I V Gilevich, A S Sotnichenko, D D Karal-Ogly, E A Gubareva, E V Kuevda, I S Polyakov, B A Lapin, S V Orlov, V A Porkhanov, V P Chekhonin
Biological compatibility of a tissue engineered construct of the trachea (synthetic scaffold) and allogenic mesenchymal stem cells was studied on laboratory Papio hamadryas primates. Subcutaneous implantation and orthotopic transplantations of tissue engineered constructs were carried out. Histological studies of the construct showed chaotically located filaments and mononuclear cells fixed to them. Development of a fine connective tissue capsule was found at the site of subcutaneous implantation of the tissue engineered construct...
April 16, 2018: Bulletin of Experimental Biology and Medicine
S V Michurina, S I Kolesnikov, A L Bochkareva, S A Arkhipov, I Yu Ishchenko
The expression of apoptosis regulators (proapoptotic protein Bad and anti-apoptotic protein Bcl-2) was analyzed and Bcl-2/Bad ratio in the follicular apparatus of the rat ovary was determined on day 3 after hyperthermia (rectal temperature 43.5°C). Hyperthermia in the catabolic phase leads to different degrees of activation of the molecular "switches" of apoptosis in cells of ovarian follicular epithelium. This was seen from increased intensity of immunohistochemical staining for Bad protein against the background of more pronounced expression of Bcl-2 protein...
April 16, 2018: Bulletin of Experimental Biology and Medicine
S Kasuda, R Kudo, K Yuui, Y Sakurai, K Hatake
The possibility of sphingosine-1-phosphate production by induced pluripotent stem cells is examined to assess their potential in treatment of sepsis. The hematopoietic embryoid bodies were derived from the culture of 6-day-old differentiated induced pluripotent stem cells. These embryoid bodies secreted sphingosine-1-phosphate, an important bioactive lipid that regulates integrity of the pulmonary endothelial barrier, prevents elevation of its permeability, and impedes the formation of stress fibers in human endotheliocytes derived from umbilical vein...
April 16, 2018: Bulletin of Experimental Biology and Medicine
I V Maiborodin, V V Morozov, A A Anikeev, N F Figurenko, R V Maslov, V A Matveeva, V I Maiborodina
Hemorrhagic changes after subcutaneous injection of autologous bone marrow multipotent mesenchymal cells with transfected GFP gene and additionally stained cell membranes to WAG rats in the projection of ligated femoral vein were studied by fluorescent microscopy. Hemorrhages in tissues with experimental acute local venous occlusion were caused by a combination of venous hypertension with inflammation around the foreign body - the ligature used for ligation of the vein. Fibrin found in tissues together with erythrocytes in the hemorrhages could stimulate the formation of granulations and new vessels instead of damaged or thrombosed ones...
April 16, 2018: Bulletin of Experimental Biology and Medicine
V N Afonyushkin, Yu N Kozlova, I N Tromenshleger, M L Filipenko, O B Novikova
We propose an original methodological approach to discrimination of newly isolated Salmonella enterica strains with the use of Dienes test. Dienes test is used for identification of P. vulgaris and P. mirabilis strains. It consists in growth suppression by mobile bacterial strain cultures and the formation of a demarcation line (Dienes line) between the strains growing towards each other. Similarities and differences between salmonella phagotyping method and Dienes test-based discrimination of the strains are detected...
April 16, 2018: Bulletin of Experimental Biology and Medicine
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