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Bulletin of Experimental Biology and Medicine

P F Zabrodskii, M S Gromov, V V Maslyakov
Experiments on random-bred albino mice showed that NF-κB inhibitor (BAY 11-7082) and β2-adrenoreceptor agonist (dexmedetomidine hydrochloride) significantly reduced mouse mortality in 4 and 24 h after sepsis modeling (intraperitoneal administration of E. coli) by reducing blood levels of proinflammatory cytokines TNFα, IL-1β, and IL-6. The combined administration of NF-κB inhibitor and β2-adrenoreceptors agonist have an additive effect.
August 18, 2018: Bulletin of Experimental Biology and Medicine
Z V Bakaeva, N V Ermakova, O V Mankaeva, D S Sveshnikov, A E Severin, A N Sinel'nikova, Yu P Starshinov, I V Radysh, V I Torshin, D A Frolov, I G Kozlov
We studied the effect of collagen fragments (PGP and AcPGP) on serum content of epinephrine, corticosterone, and IL-1β in rats subjected to water-immersion stress. The degree of local inflammation accompanying ulceration was assessed by IL-1β production by ln. gastricus caudalis cells. In 1 h, the sharp increase in hormone concentrations in the blood of stressed animals reflected the high stress intensity. Intranasal administration of PGP reduced the area of stress-induced ulcers by 63%, prevented the increase in the levels of stress hormones and the main proinflammatory cytokine in rat blood...
August 18, 2018: Bulletin of Experimental Biology and Medicine
Ya Sh Schwartz, O M Dolganova, M I Rudina, I A Pyshnaya, N P Bgatova
We studied the effect of extracellular vesicular particles generated during apoptosis by macrophages of M0, M1 and M2 phenotypes on spontaneous and LPS-stimulated production of NO. The fractions of apoptotic bodies and apoptotic microvesicles were obtained in the primary cultures of peritoneal macrophages undergoing apoptosis. The effect of these microparticles on LPS-induced proinflammatory response of recipient macrophages critically depends on the initial phenotype of "donor" macrophages. Microvesicles and especially apoptotic bodies from M1 macrophages stimulate basal NO production...
August 18, 2018: Bulletin of Experimental Biology and Medicine
D D Bezmaternykh, M E Mel'nikov, E D Petrovskii, L I Kozlova, M B Shtark, A A Savelov, O S Shubina, K A Natarova
Depression is associated with changes in the pattern of interaction of cerebral networks, which can reflect both existing symptoms and compensatory processes. The study is based on analysis of resting state fMRI data from 15 patients with mild depression and 19 conventionally healthy individuals. From fMRI signal recorded at rest for 4 min, the independent components were reconstructed. The intergroup differences and dynamics of functional connectivity from the first to the second recording were analyzed. Initially, depressive patients demonstrated weaker connectivity between cerebellar declive network (CN) and left central executive network (CEN) and also sensorimotor network (SMN); left CEN and primary visual network (PVN)...
July 14, 2018: Bulletin of Experimental Biology and Medicine
D I Vodolazhsky, D S Kutilin, Kh A Mogushkova, O I Kit
Cancer-testis antigens, effective markers of tissue malignant transformation, are characterized by heterogonous transcription depending on the pathological features of breast cancer. We performed screening of transcription profile of cancer-testis antigens specific for breast tumor tissues in female patients with and without regional metastasis. The relative expression of 16 genes (MAGEA1, MAGEA2, MAGEA3, MAGEA4, MAGEB1, MAGEB2, GAGE1, GAGE3, GAGE4, MAGEC1, BAGE, XAGE3, NY-ESO1, SSX2, SYCP1, and PRAME1) was analyzed by RT-qPCR method in biopsy specimens of the mammary gland tissues obtained during surgery from 25 patients...
July 14, 2018: Bulletin of Experimental Biology and Medicine
O G Bernikova, K A Sedova, S N Kharin, Ya E Azarov
The effects of therapeutic or preventive-therapeutic administration of water-soluble echinochrome analog U-441 on arrhythmia severity assessed by a set of myocardial spatio-temporal depolarization and repolarization parameters were examined on the model of acute myocardial ischemia in cats. Coronary occlusion increased activation time and decreased repolarization time in the ischemic zone; in addition, it increased both global and borderline (local) dispersions of repolarization. The linear regression model showed that only activation time values measured at the initial state and at termination of occlusion were associated with total arrhythmia score during ischemia (regression coefficient β=0...
July 13, 2018: Bulletin of Experimental Biology and Medicine
M N Zamotaeva, V I Inchina, V V Konorev, Yu V Kuznetsov, N D Kuz'michev
The possibility of correction of morphological changes in the myocardium and biochemical parameters of the blood with 3-hydroxypyridine acetylcysteinate in a dose of 25 mg/kg was studied in the model of doxorubicin-induced chronic heart failure in rats. It was found that 3-hydroxypyridine acetylcysteinate in a dose of 25 mg/kg produced less pronounced cardio-protective effect in experimental chronic heart failure than 3-hydroxypyridine succinate.
July 13, 2018: Bulletin of Experimental Biology and Medicine
N N Sazhina, N M Evteeva, V N Titov
The kinetics of ozone interaction with 14 saturated and unsaturated fatty acids with different molecular structures was studied and velocity constants of these reactions were measured. The relationships between the measured constants and some parameters of fatty acid (length of the aliphatic chain, number of double bonds, location of the double bonds, and its configuration) were detected. The highest velocity constant is recorded for monounsaturated oleic fatty acid: 2.58×105 (M×sec)-1 . Other studied fatty acids had 1...
July 13, 2018: Bulletin of Experimental Biology and Medicine
A S Vetchinova, V V Simonova, E V Novosadova, E S Manuilova, V V Nenasheva, V Z Tarantul, I A Grivennikov, L G Khaspekov, S N Illarioshkin
We performed a cytogenetic analysis of the results of CRISPR/Cas9-correction of G2019S mutation in LRRK2 gene associated with Parkinson's disease. Genome editing was performed on induced pluripotent stem cells derived from fibroblasts of a patient carrying this mutation. A mosaic variant of tetraploidy 92 XXYY/46,XY (24-43% cells from various clones) was found in neuronal precursors differentiated from the induced pluripotent stem cells after gene editing procedure. Solitary cases of translocations and chromosome breaks were observed...
July 13, 2018: Bulletin of Experimental Biology and Medicine
N P Bgatova, S A Bakhbaeva, Yu S Taskaeva, V V Makarova, Yu I Borodin
Structural changes in the liver of CBA mice were studied during the development of experimental hepatocarcinoma-29 inoculated into the hip. A decrease in the volume density of hepatocyte cytoplasm, mitochondria, endoplasmic reticulum, and lipid inclusions and an increase in the volume density of lysosomal structures during tumor growth were observed. All stages of intracellular autophagy were recorded by the method of electron microscopy. These stages included the appearance of autophagosomes, autophagolysosomes, and secondary lysosomes in the hepatocyte cytoplasm...
July 13, 2018: Bulletin of Experimental Biology and Medicine
A A Byvalov, M A Malkova, A V Chernyad'ev, L G Dudina, S G Litvinets, E A Martinson
Incubation of Yersinia pseudotuberculosis cells grown on a solid medium with pseudotuberculous diagnostic bacteriophage for 20 min at 37oC led to a significant decrease in the concentration of both components of the system. This effect was absent when the bacteria were grown in a fluid medium. At the same time, this incubation regimen promoted vesicle formation and typical morphological changes in bacteria grown in both surface and suspension cultures. Co-incubation of the bacteriophage with suspension of vesicles isolated from the suspension culture of Y...
July 13, 2018: Bulletin of Experimental Biology and Medicine
I V Babenkova, A N Osipov, Yu O Teselkin
Dihydroquercetin is a natural flavonoid with antioxidant properties that are determined by its ability to scavenge free radicals and chelate transition metal ions. Chemiluminescent studies showed that iron (II) ions in complex with dihydroquercetin are inactive and cannot catalyze decomposition of hydrogen peroxide (Fenton reaction).
July 13, 2018: Bulletin of Experimental Biology and Medicine
M V Vasin, T S Gan'shina, R S Mirzoyan, L A Semenova, L V Koroleva, R V Afanas'ev, I B Ushakov
We studied the effect of radioprotector indralin (B-190) alone or in combination with monizol on BP and HR in rabbits, reduction of blood supply and spleen weight in rats and (CBA×C57Bl/6)F1 hybrids mice, and on blood loss from a wound on tip of the tail in mice. Being an α1-adrenomimetic, indralin caused hypertensive reaction with the development of bradycardia, reduced blood supply and spleen weight, and sharply reduced blood loss from the wound. Monizol as nitrate reduced BP without affecting HR and reduced blood loss from the wound...
July 13, 2018: Bulletin of Experimental Biology and Medicine
O V Kokorev, V N Khodorenko, S G Anikeev, V E Gunter
The dynamics of the development of the pancreatic islet cells in the porous TiNi scaffold was studied by electron microscopy. Changes in the metabolism of glucose and glycosylated hemoglobin and parameters of the peripheral blood and bone marrow were shown after transplantation of pancreatic islet cells on porous permeable TiNi scaffold during alloxan-induced diabetes. The cells administered on the porous biocompatible scaffold produced more prolonged anti-diabetic effect and normalized hemopoiesis parameters in comparison with their intraperitoneal administration...
July 13, 2018: Bulletin of Experimental Biology and Medicine
N L Lila, S K Sudakov
We developed a new experimental model for studies of orientation and exploratory behavior in humans. This method allows analysis of variants of orientation and exploratory behavior in humans, including risky behavior, and cognitive and individual typological parameters. The model allows distinguishing examinees with high and low orientation and exploratory activity, which helps to determine their psychoemotional status. Surprisingly, individuals in emotional strain demonstrated longer orientation and exploratory behavior...
July 12, 2018: Bulletin of Experimental Biology and Medicine
V M Ukrainskaya, T V Bobik, A Argentova-Stevens, E A Slutskaya, R S Kalinin, A G Gabibov, A V Stepanov
Death receptor 5 (DR5) is a promising target for antitumor therapy due to its high expression on different tumor cells. Resistance of various tumor cells against TRAIL, a natural ligand for the death receptors, reduces its therapeutic potential and prompts the search for novel agonists at these receptors. Previous screening across the combinatorial peptide library yielded a peptide sequence KVVLTHR that specifically binds DR5. Incorporation of this sequence into TNFα resulted in binding DR5 with mutant protein TNFα-mut and appearance of cytotoxicity against lymphoma cells...
July 12, 2018: Bulletin of Experimental Biology and Medicine
A A Ivanov, T I Danilova, O P Popova, A I Erohin, E S Semenihina
We carried out a comparative study of the features of osteogenesis from the progenitor osteogenic periosteal cells in rabbit and human. At the initial stages, high osteogenic potential of both human and rabbit periosteal cells was observed. However, at the later stages, the cell response favors resorption of the new bone tissue formed from periosteal cells in rabbits, but does not affect the bone tissue formed from human progenitor osteogenic periosteal cells. These functional characteristics of rabbit periosteal cells should be considered when planning the experiment...
July 12, 2018: Bulletin of Experimental Biology and Medicine
G A Abalakov, P V Sutyagin
A previously popular antiarrhythmic drug moricizine (ethmozine) is known for its blocking action on the fast sodium channels in cardiomyocytes. Its effects were examined only in isolated cardiomyocytes or in vivo. Here, the effect of moricizine (10 μM) was examined in vitro on perfused right atrial preparation, where it completely reproduced all the previously observed phenomena and disturbed electrical coupling between the working cardiomyocytes in 35.3±3.4 min, which arrested generation of action potentials...
July 12, 2018: Bulletin of Experimental Biology and Medicine
A F Korystova, L N Kublik, Yu A Kim, M Kh Levitman, V V Shaposhnikova, Yu N Korystov
The time course of angiotensin-converting enzyme activity in the rat aorta after fractionated exposure to ionizing radiation and the effects of dihydroquercetin and fucoidin on this parameter were studied. Male Wistar rats were exposed to single or repeated (fractionated) X-ray radiation in a dose of 2.5 Gy at 200 kV. Activity of angiotensin-converting enzyme in aorta segments was evaluated 2 h after the last exposure by hydrolysis of hippuryl-histidineleucin substrate. Enzyme activity in the rat aorta was higher than normally after all the studied doses of fractionated exposure (2...
July 12, 2018: Bulletin of Experimental Biology and Medicine
N V Semenova, I M Madaeva, T A Bairova, R M Zhambalova, O V Kalyuzhnaya, M A Rashidova, L I Kolesnikova
We carried out a comparative analysis of circadian rhythms of melatonin secretion in Caucasian menopausal women with and without insomnia depending on the 3111T/C polymorphism of the Clock gene. Melatonin levels was measured in the saliva 4 times a day (06.00-07.00, 12.00-13.00, 18.00-19.00, and 23.00-00.00 h). Carriers of the TT genotype with insomnia demonstrated significantly higher level of melatonin in the early morning hours compared to the carriers of the minor allele C (12.60±7.58 and 8.98±8.62 pg/ml, respectively, p=0...
July 12, 2018: Bulletin of Experimental Biology and Medicine
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