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Bulletin of Experimental Biology and Medicine

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https://www.readbyqxmd.com/read/28730390/expression-of-type-i-cannabinoid-receptors-at-different-stages-of-neuronal-differentiation-of-human-fibroblasts
#1
M Yu Bobrov, V V Bezuglov, L G Khaspekov, S N Illarioshkin, E V Novosadova, I A Grivennikov
Differential expression of type 1 cannabinoid receptors (CR1) was evaluated at different stages of human skin fibroblast transformation into terminally differentiated neurons. Immunocytochemical staining detected no CR1 on fibroblasts, but their transformation into induced pluripotent stem cells was accompanied by marked stimulation of CR1 expression. In neuronal precursors, the receptors were located mainly on cell bodies and at the base of their processes. This distribution was retained at the terminal stage of differentiation of induced pluripotent stem cells into neurons...
July 21, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726189/changing-the-properties-of-multipotent-mesenchymal-stromal-cells-by-ifn%C3%AE-administration
#2
N A Petinati, N M Kapranov, A E Bigil'deev, M D Popova, Yu O Davydova, I V Gal'tseva, N I Drize, L A Kuz'mina, E N Parovichnikova, V G Savchenko
We studied changes in the population of human multipotent mesenchymal stromal cells activated by IFNγ. The cells were cultured under standard conditions; IFNγ was added in various concentrations for 4 h or over 2 passages. It was shown that the total cell production significantly decreased after long-term culturing with IFNγ, but 4-h exposure did not affect this parameter. After 4-h culturing, the expression levels of IDO1, CSF1, and IL-6 increased by 300, 7, and 2.4 times, respectively, and this increase persisted 1 and 2 days after removal of IFNγ from the culture medium...
July 19, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28730389/cardiovascular-effects-of-high-molecular-weight-compounds-of-humic-nature
#3
M V Zykova, M V Belousov, T V Lasukova, A S Gorbunov, L A Logvinova, A M Dygai
The active ingredient extracted from the peat humic substances was characterized by physicochemical parameters evaluated by UV- and IR-spectroscopy, titration, and elemental (C, H, N) analysis. The cardiovascular effects of this ingredient were examined on isolated Langendorff-perfused rat heart. It was found that the active substance in a concentration range of 0.01-0.1 mg/ml produced a vasodilating effect; in addition, it decreased the end-diastolic and left-ventricular developed pressures.
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28730388/assessment-of-oxidative-status-of-the-brain-and-blood-plasma-in-rats-with-modeled-focal-cerebral-ischemia-reperfusion-injury
#4
A A Devyatov, T N Fedorova, S L Stvolinskii, M A Belousova, O S Medvedev, V A Tutelyan
Parameters of the oxidative status of the brain and blood plasma were measured in rats 24 h after 1-h focal cerebral ischemia. In the brain of rats exposed to cerebral ischemia, activities of superoxide dismutase and catalase were elevated. Ischemia reduced the total antioxidant activity of the brain and the levels of malonic dialdehyde and protein carbonyl derivatives. In the blood plasma of experimental rats, superoxide dismutase activity and malonic dialdehyde level increased and total antioxidant activity decreased, i...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28730387/dynamics-of-the-pro-oxidant-antioxidant-system-parameters-in-wound-discharge-and-plasma-in-experimental-purulent-wound-during-its-technological-liquid-phase-treatment
#5
I M Bykov, A A Basov, V V Malyshko, S S Dzhimak, S R Fedosov, A V Moiseev
The dynamics of the pro-oxidant/antioxidant system of rabbits was studied at the local (in exudate) and systemic (blood) levels during therapy of purulent wounds in liquid medium with the use of a programmed device and the efficiency of the proposed technological method was compared with that of classical methods for the treatment of these wounds. More rapid recovery of the indicators of the pro-oxidant/antioxidant system to physiological values in the postoperative period was observed after treatment by the proposed method (7 days vs...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726211/genetic-engineering-of-native-chain-combinations-of-b-cell-repertoires-on-the-surface-of-methylotrophic-yeasts-pichia-pastoris
#6
T V Bobik, E M Shurdova, I V Smirnov, N A Ponomarenko, E N Khurs, V D Knorre, A G Gabibov
We designed genetic constructs for exposing Fab-fragment library of natively paired single cell B-cell receptors on the surface of Pichia pastoris yeast cells. We have previously obtained the A17 antibody in our laboratory [6]. In this study we showed that the newly designed genetic constructs provide a compatible level of A17 antibody Fab fragment on the surface of yeast cells as well as in the case of vectors containing DNA fragments corresponding to each chain of the antibody. The data suggest that the developed approach for constructing immunoglobulin gene libraries is adequate and fully convenient for studying properties of the real human B-lymphocyte repertoire...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726210/sodium-dodecyl-sulfate-cytotoxicity-towards-hacat-keratinocytes-comparative-analysis-of-methods-for-evaluation-of-cell-viability
#7
A L Rusanov, N G Luzgina, A V Lisitsa
Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726209/characteristics-of-mel-ibr-melanoma-line-subclone-after-treatment-with-chicken-embryo-extract
#8
N M Suraeva, L F Morozova, O O Ryabaya, Yu A Khochenkova, A V Samoilov, O S Burova, N V Golubtsova, A E Barmashov, M A Baryshnikova
We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726208/immunolocation-of-heme-oxygenases-in-the-walls-of-cerebral-arteries-of-various-diameters-in-rats
#9
V M Chertok, A E Kotsyuba
The distribution of two enzymes involved in the formation of carbon monoxide, heme oxygenases 1 and 2, in the pial branches of orders I-V of the middle cerebral artery basin and in intracerebral vessels was studied in adult Wistar rats. Immunohistochemical studies detected hemeoxygenase-2 in the endothelium of the small pial and intracerebral arterioles and in myocytes of pial branches I-III. Heme oxygenase 1, an inducible form of the enzyme, is normally not expressed in the cerebral vessels, but the enzyme is expressed in response to sodium metaarsenite...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726207/approaches-to-controlled-co-amplification-of-genes-for-production-of-biopharmaceuticals-study-of-the-insertion-and-amplification-dynamics-of-genetic-cassettes-in-the-genome-of-chinese-hamster-ovary-cells-during-co-expression-of-compatible-pair-of-plasmids
#10
S V Kovnir, N A Orlova, Yu А Khodak, M P Kondrashova, A G Gabibov, K G Skryabin, A I Vorobiev, I I Vorobiev
Plasmid vector family p1.1 based on non-coding regions of Chinese hamster housekeeping gene EEF1A and concatemer of Epstein-Barr virus terminal repeat increases the frequency of genome integration and provides rapid amplification of the target genes in the genome. For a pair of fluorescent proteins eGFP and mCherry it was shown that p1.1 vectors bearing dihydrofolate reductase and glutamine synthetase selection markers upon co-transfection into CHO DG44 cell line allow obtaining a polyclonal cell population in which ~70% of cells express both genes...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726206/preparation-of-recombinant-serpin-from-red-king-crab-paralithodes-%C3%B1-amtschaticus-for-biomedical-research-purposes
#11
N N Kostin, N A Ponomarenko, V A Isaev, G N Rudenskaya, T V Bobik, A G Gabibov, I V Smirnov
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726205/dipeptidyl-peptidase-4-inhibitors-diprotin-a-and-sitagliptin-administered-on-weeks-2-3-of-postnatal-development-modulate-monoamine-metabolism-in-the-striatum-of-adult-rats
#12
N N Khlebnikova, E V Orshanskaya, V B Narkevich, V S Kudrin, N A Krupina
The levels of monoamines and their metabolites in brain structures of adult (3-month-old) rats with emotional and motivational disorders induced by inhibitors of dipeptidyl peptidase 4 (DPP-4; EC 3.4.14.5) diprotin A and sitagliptin on weeks 2-3 of postnatal development (postnatal days 5-18) were studied by HPLC with electrochemical detection. A significant decrease in the level of serotonin metabolite, 5-hydroxyindoleacetic acid, and a pronounced tendency towards reduced serotonin level were detected in the striatum of rats in both study groups...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726204/association-of-level-of-proprotein-convertase-subtilisin-kexin-type-9-with-intima-media-thickness-in-patients-with-familial-hypercholesterolemia
#13
K S Benimetskaya, Yu I Ragino, E V Shakhtshneider, K V Makarenkova, Yu V Shchepina, E M Stakhneva, M I Voevoda
We studied association of PCSK9 protein with the carotid artery intima-media thickness in patients with familial hypercholesterolemia (N=53; age 49.9±6.9 years) treated with statins. Blood level of PCSK9 protein was measured by ELISA; ultrasonography of the carotid arteries with measurement of the thickness of the intima-media complex of the common carotid arteries in the distal segment for 10 mm from the bifurcation on the far wall of the vessel was performed in on-line mode. The mean values were calculated for both sides, the maximum mean value was included in the analysis...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726203/cysteine-containing-peptides-stimulate-monocyte-migration-through-nadph-oxidase-activation
#14
T L Krasnikova, T I Arefieva, E A Pylaeva, M V Sidorova
We analyzed migration of monocytes under the effect of apocinin (NADPH inhibitor) and PD98059 (blocker of extracellular MEK/ERK kinase involved in Nox4 oxidase-mediated migration of monocytes). Migration of monocytes stimulated by cysteine-containing peptides (fragments of chemokines with free thiol group MCP-1 and fractalkine) was completely inhibited by apocinin and MEK/ERK blocker. It is assumed that the stimulating effect of cysteine-containing peptides on monocyte migration is mediated by the NADPH-oxidase system, in particular, Nox4...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726202/experimental-assessment-of-the-effect-of-kagocel-on-reproductive-function-in-pubertal-male-rats
#15
T G Borovskaya, M E Poluektova, A V Vychuzhanina, V A Mashanova, Yu A Shchemerova
We studied possible toxic effects of antiviral drug Kagocel on reproductive function in pubertal male rats. The drug was administered in therapeutic and 10-fold higher doses throughout the spermatogenesis cycle (48 days). Kagocel did not reduce mating and fertilizing capacities, did not suppress spermatogenesis, and had no toxic effects on the offspring. The results characterize Kagocel as a drug with a broad reproductive safety profile and demonstrate that the age limits for using Kagocel in pediatric practice can be extended...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726201/delayed-results-of-experimental-afobazole-therapy-in-rats-after-acute-myocardial-infarction
#16
S A Kryzhanovskii, I B Tsorin, V N Stolyaruk, E O Ionova, M B Vititnova
Delayed cardioprotective effects of anxiolytic Afobazole (15 mg/kg, intraperitoneally for 14 days) were evaluated using dynamic echocardiographic recordings on days 2, 15, 56, and 98 after experimental myocardial infarction modeling (rat model of acute myocardial ischemia). The cardiotropic activity of Afobazole is assumed to be related to its agonistic effects on σ1 receptor of cardiomyocytes. It was found that animals treated with Afobazole had no signs of heart failure by the end of observation, as evidenced by left ventricular ejection fraction...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726200/intranasal-insulin-restores-metabolic-parameters-and-insulin-sensitivity-in-rats-with-metabolic-syndrome
#17
K V Derkach, A O Ivantsov, O V Chistyakova, I B Sukhov, D M Buzanakov, A A Kulikova, A O Shpakov
We studied the effect of 10-week treatment with intranasal insulin (0.5 IU/day) on glucose tolerance, glucose utilization, lipid metabolism, functions of pancreatic β cells, and insulin system in the liver of rats with cafeteria diet-induced metabolic syndrome. The therapy reduced body weight and blood levels of insulin, triglycerides, and atherogenic cholesterol that are typically increased in metabolic syndrome, normalized glucose tolerance and its utilization, and increased activity of insulin signaling system in the liver, thus reducing insulin resistance...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726199/a-study-of-the-protective-properties-of-an-antibody-based-antidote-metabolizing-organophosphorus-pesticide-paraoxon
#18
Yu A Mokrushina, A V Stepanova, V A Palikov, A V Stepanov, G I Belous, I A Dyachenko, V D Knorre, I V Smirnov, A G Gabibov
A catalytic antibody A17 and its mutants highly efficiently interact with organophosphorus pesticide paraoxon. In this work, we studied the protective properties of antibody A17-K47 in paraoxon poisoning using a mouse model. The optimal paraoxon dose simulating the acute toxic effect of organophosphorus compounds was 550 μg/kg. The pharmacokinetic parameters of A17-K47 antibody were t1/2distr =7.2±1.4 min, t1/2el =330±20 min. The antibody did not cause toxic effects when administered at a ten-fold calculated therapeutic dose (610 mg/kg)...
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726198/anti-inflammatory-and-analgesic-activities-of-the-complex-of-flavonoids-from-lychnis-chalcedonica-l
#19
Yu V Nesterova, T N Povet'eva, L N Zibareva, N I Suslov, E P Zueva, S G Aksinenko, O G Afanas'eva, S G Krylova, E N Amosova, O Yu Rybalkina, K A Lopatina
Anti-inflammatory and analgesic activities of the complex of flavonoids from Lychnis chalcedonica L. were studied in the models of acute aseptic inflammation induced by carrageenan, histamine, and serotonin and acetic acid-induced painful chemical stimulation. It is demonstrated that course treatment with flavonoids derived from Lychnis chalcedonica L. produced a stable pharmacological effect comparable with that of the reference anti-inflammatory drug diclofenac.
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28726197/effect-of-phenibut-and-glufimet-a-novel-glutamic-acid-derivative-on-respiration-of-heart-and-brain-mitochondria-from-animals-exposed-to-stress-against-the-background-of-inducible-no-synthase-blockade
#20
V N Perfilova, T A Popova, I I Prokofiev, I S Mokrousov, O V Ostrovskii, I N Tyurenkov
Increased oxygen consumption by heart and brain mitochondria in the absence of ADP and reduced mitochondrial respiration in the presence of ADP were observed in rats exposed to stress simulated by suspension by the dorsal neck skin fold for 24 h, which attests to uncoupling of substrate oxidation and ATP synthesis and can cause electron drain from the respiratory chain, formation of ROS, and oxidative damage to cell structures. Blockade of inducible NO synthase with aminoguanidine (single intraperitoneal dose of 50 mg/kg before stress exposure) increased coupling of respiration and oxidative phosphorylation in heart and brain mitochondria of rats exposed to immobilization-painful stress, which was especially pronounced in cardiomyocytes...
July 18, 2017: Bulletin of Experimental Biology and Medicine
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