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Bulletin of Experimental Biology and Medicine

E A Safonova, K A Lopatina, T G Razina, E P Zueva, E P Fedorova, A M Gur'ev, M V Belousov
The experiments on C57Bl/6 mice with Lewis lung carcinoma showed that addition of Tussilago farfara L. polysaccharides to conventional cisplatin/paclitaxel polychemotherapy moderated neutropenia caused by antitumor therapy and increased its efficiency. The stimulating effect of polysaccharides on the granulopoietic lineage cells is comparable with that of recombinant CSF Neupogen.
December 2018: Bulletin of Experimental Biology and Medicine
T V Kozyreva, A A Evtushenko, I P Voronova, G M Khramova, V P Kozaruk
Experiments on rats showed that activation of the peripheral ion channel TRPM8 with menthol and rapid cooling (decrease in core temperature by 3°C) led to 1.5-fold activation of the expression of TRPV3 ion channel gene in the posterior hypothalamus, but had no effect on the expression of this gene in the anterior hypothalamus. Neither stimulation of peripheral TRPМ8, nor acute cooling affected the expression of genes for other thermosensitive ion channels (TRPV1, TRPV2, TRPV4, TRPA1, and TRPМ8) in the hypothalamus...
December 2018: Bulletin of Experimental Biology and Medicine
M L Blagonravov, E V Medvedeva, A A Bryk, V A Goryachev, A E Rabinovich, A S Letoshneva, E A Demurov
We presented the results of our study of chronostructure of BP, HR, electrolyte excretion, and locomotor activity under conditions of "free-run rhythm" (light deprivation). In adult male Wistar-Kyoto (normotensive) and SHR (spontaneously hypertensive) rats, BP, biopotentials of the heart (ECG), and locomotor activity were recorded over 24 h by telemetric monitoring and the rate of excretion of electrolytes (Na+ , K+ , Ca2+ , and Mg2+ ) during the nighttime and daytime hours was measured. It was found that under free-run rhythm, 24-h profiles of BP, HR, excretory function of the kidneys, and locomotor activity underwent more considerable changes in normotensive Wistar-Kyoto rats in comparison with hypertensive SHR rats...
December 2018: Bulletin of Experimental Biology and Medicine
P V Kochubei, G V Kopylova, D V Shchepkin, S Yu Bershitskii
The molecular mechanism of violation of the contractile function of skeletal muscles caused by oxidative damage to myosin is not fully understood. Using permeabilized fibres from fast (m. psoas) and slow (m. soleus) rabbit muscles, we studied the effect of myosin oxidation on the mechanism of force generation and its calcium regulation. It was found that this treatment simultaneously reduces the maximum force and fibers stiffness without affecting their calcium sensitivity. This suggests that the mechanism of oxidation-related impairment the force-generating ability of fibers consists in suppression of myosin cross-bridges formation and does no affect the characteristics of actin-myosin interaction...
December 2018: Bulletin of Experimental Biology and Medicine
V P Nuzhny, N A Kibler, D N Shmakov
We studied electrophysiological mechanisms of ventricular arrhythmias in dogs (n=7) under conditions of antiorthostatic hypokinesia (head-down tilt 45°). Abnormal transmural heterogeneity of repolarization in the base and apex of the left ventricle and increased dispersion of myocardial repolarization were revealed. By minute 30 of antiorthostatic hypokinesia, an increase in the duration of repolarization was revealed after a period of ventricular arrhythmia in all segments and regions of heart ventricles, which was accompanied by impairment of the pumping function of the heart...
December 2018: Bulletin of Experimental Biology and Medicine
E G Skurikhin, V A Krupin, O V Pershina, E S Pan, L A Ermolaeva, A V Pakhomova, O Yu Rybalkina, N N Ermakova, E S Khmelevskaya, O E Vaizova, M S Zhukova, A S Pozdeeva, V E Skurikhina, V E Goldberg, A M Dygai
We studied the effects of elastase, cigarette smoke extract, D-galactosamine hydrochloride, and tyrosine kinase inhibitor SU5416 on endothelial progenitor cells and angiogenesis precursors, as well as on Notch-1 expression by immature endothelial cells. Simultaneously with pulmonary emphysema, different damaging factors with diverse mechanisms of action caused pathological changes in the microvascular network of the lungs and destroyed the alveolar endothelium in female C57Bl/6 mice. D-galactosamine hydrochloride disturbed mobilization of endothelial progenitor cells expressing VEGFR (CD45-CD309+ ) and angiogenesis progenitors (CD45- CD309+ CD117+ ) and their migration into emphysema expanded lungs...
December 2018: Bulletin of Experimental Biology and Medicine
E A Safonova, K A Lopatina, A V Vychuzhanina, V A Mashanova, T G Razina, T G Borovskaya, E P Zueva, A M Gur'ev, M V Belousov
Using DNA comet assay we found that polysaccharides from Tussilago farfara L. reduced the intensity of polychemotherapy-induced apoptosis and DNA damage in bone marrow cells and small intestinal epithelium of C57Bl/6 mice, which attested to genoprotective properties of these polysaccharides.
December 2018: Bulletin of Experimental Biology and Medicine
L A Dzikovskaya, K T Erimbetov, I P Grosheva, E V Bondarenko, A Ya Goncharova
We studied the effect of Fe2+ ions in polymerized hemoglobin (Krunidon blood substitute) and in molecular hemoglobin (Sigma) on OH• radical initiation in the Fenton system. It was found that polymerized hemoglobin, as a component of Krunidon preparation, in contrast to hemoglobin tetramer, did not intensify OH• radical generation. The oxidant potential of Krunidon was evaluated in vivo by measuring malondialdehyde level in dog blood plasma after repeated intravenous administration (5 days in a dose of 114 mg/kg) as a biomarker...
December 2018: Bulletin of Experimental Biology and Medicine
A A Tiunova, N V Komissarova, K V Anokhin
We studied the effects of light and non-specific sound stimulation of domestic chick embryos on their filial preference as well as on the expression of two transcriptional factors c-Fos and Egr-1 and neurotrophin BDNF in the embryo brain. Prenatal light stimulation increased preference of the "natural" object, thus producing a priming effect. In the brain of E19 embryos, c-Fos and Egr-1 were expressed at a high basal level and neither light nor sound stimulation affected the number of cells expressing these factors...
December 2018: Bulletin of Experimental Biology and Medicine
A V Gorskaya, O N Antosyuk, O S Ermakova, A S H Mukhametzyanova, K N Kochkina
The genotoxic effects of heterocyclic compounds were evaluated on the basis of genetic and toxicological characteristics of a biological model of Drosophila melanogaster. Analysis of the viability parameters (fertility, progeny mortality) showed that of 6 tested substance, substance No. 3 exhibited minimum toxicity. After application of substances No. 1 and No. 5 in the studied concentrations, the number of survived flies was insufficient for further analysis, which attested to high toxicity of these substances...
December 2018: Bulletin of Experimental Biology and Medicine
V A Serebryakova, O I Urazova, V V Novitsky, A I Vengerovskii, T E Kononova
The effects levofloxacin (fluoroquinolone) and vaccinal BCG strain on cytokine production by blood mononuclear leukocytes was studied in patients with infiltrative pulmonary tuberculosis. Combined treatment with levofloxacin and vaccinal BCG strain suppressed the production of TNFα in drug-resistant pulmonary tuberculosis and production of IL-12 and IFNγ in drug-sensitive tuberculosis.
December 2018: Bulletin of Experimental Biology and Medicine
T G Smirnova, A Yu Savochkina, I I Dolgushin, K V Nikushkina, I V Samuseva
We studied functional activity of neutrophilic granulocytes and monocytes isolated from the peripheral blood of women during the follicular and luteal phases of the menstrual cycle. It was shown that phagocytic activity of neutrophilic granulocytes increases, their intracellular oxygen-dependent bactericidal activity decreases, and the number of monocyte extracellular traps increases in women in the luteal phase of the menstrual cycle in comparison with the follicular phase.
December 2018: Bulletin of Experimental Biology and Medicine
N V Apanovich, M V Peters, P V Apanovich, A S Markova, B Sh Kamolov, V B Matveev, A V Karpukhin
We analyzed association of the levels of VEGFA, RAF1, and mTOR gene expression in the tissue of clear-cell renal cell carcinoma (ccRCC) with tumor metastasizing. Significant association with metastases was found only for VEGFA gene: OR=6.641, 95%CI=2.111-20.696. The risk of metastasis associated with reduced expression of VEGFA gene - 2.467, 95%CI=1.238-4.915. An association of VEGFA gene expression with the time to the metastasis appearance was revealed (p=0.0005). Reduced expression of the VEGFA gene is associated with reduction of the time to metastasis appearance; the median of this time is shifted from 46 to 2 months...
December 2018: Bulletin of Experimental Biology and Medicine
E A Braga, V I Loginov, E A Filippova, A M Burdennyi, I V Pronina, T P Kazubskaya, D S Khodyrev, D O Utkin, D N Kushlinskii, L V Adamyan, N E Kuslinskii
The study was designed to determine genes of microRNAs hypermethylated in malignant ovarian tumors and to select new diagnostic and prognostic markers of the disease and effective system of markers. Using methyl-specific PCR and a representative sample of 54 ovarian cancer specimens, we determined 5 microRNA genes (MIR-34b/c, MIR-9-1, MIR-124-3, MIR-129-2, and MIR-107) hypermethylated in the majority of tumor samples in comparison with paired samples of histologically unchanged tissue (48-57% vs. 4-19%, p<0...
December 2018: Bulletin of Experimental Biology and Medicine
O V Kazakov, A V Kabakov, A F Poveshchenko, T V Raiter, D N Strunkin, O V Poveshchenko, A P Lykov, V I Konenkov
The morphometric analysis of mesenteric lymph nodes was carried out in female Wistar rats with chemically induced breast cancer. In control rats with untreated breast cancer, the volume of the system of sinuses increased in parallel with the appearance of morphological signs of suppression of cell-mediated immunity, inhibition of humoral immunity, and macrophage reaction. Against the background of chemotherapy, we observed a decrease in the volume of paracortex and lymphoid nodules, suppression of proliferative activity of lymphoid cells in paracortical and B-cell zone, and a decrease in macrophage content...
December 2018: Bulletin of Experimental Biology and Medicine
G V Kakurina, I V Kondakova, L V Spirina, E S Kolegova, E E Shashova, O V Cheremisina, V A Novikov, E L Choinzonov
The model of head and neck squamous cell carcinoma (HNSCC) was used to study the expression of genes encoding actin-binding proteins depending on the type of cell motility. The expression of SNAIL1 and CAPN2 mRNA in HNSCC tissue was higher than in specimens of dysplastic epithelium of the larynx and hypopharynx, which can be explained by activation of mesenchymal and amoeboid types of cell motility. In biopsy material of HNSCC patients with T1-2N0M0, expression of genes responsible for actin-binding proteins differed from that of patients with pretumor pathology of the larynx and hypopharynx: expression of FSCN was lower, while expressions of EZR and CAP1 were higher...
December 2018: Bulletin of Experimental Biology and Medicine
M S Makarov, M V Storozheva, N V Borovkova, I N Ponomarev
We studied proliferative activity of multipotent mesenchymal stromal cells on collagen matrices containing platelets stabilized with silver nanoparticles. Dose-dependent stimulation of the growth of stromal cells without their structural damage was observed in the presence of stabilized platelets in proportion of 20-120 mln per 105 multipotent mesenchymal stromal cells. The same doses of non-stabilized platelets produced less pronounced stimulation effect. In higher doses (≥180 mln) stabilized platelets inhibited cell proliferation and induced their damage...
December 2018: Bulletin of Experimental Biology and Medicine
V I Kirpatovskii, G D Efremov, E V Frolova
In experiments on white outbred male rats, a freshly removed (20 experiments) or cryopreserved (10 experiments) testicle from newborn rats (1-2 days after birth) was transplanted under the renal capsule after bilateral orchiectomy. In all experiments with transplantation of freshly removed testicle, it was engrafted. In 3 months, histological examination revealed the formation of mature seminiferous tubules, but spermatogenesis was blocked at the stage of spermatogonia; groups of proliferating Leydig cells in the loose connective tissue between the tubules were also seen...
December 2018: Bulletin of Experimental Biology and Medicine
N P Omelyanenko, E S Mishina, A V Kovalev, A V Volkov
Structural dynamics of the fibrous basis of the reparative regenerate during spontaneous skin wound healing comprises multiple stages, it successively transforms from one organization level to another more complex level, forms a multilevel 3D structure including molecular, supramolecular, fibrillar, fiber, and tissue elements. The formed reparative regenerate is integrated with the preserved skin, together they have common fibrous basis consisting of three parts that are different in organization of fibrous structures: atypical (central), tissue organospecific (peripheral), and transitional...
December 2018: Bulletin of Experimental Biology and Medicine
S G Fomina, D V Novikov, N V Krasnogorova, V V Novikov, S N Pleskova, A V Karaulov
We studied the influence of magnetite nanoparticles (FeO•Fe2 O3 ) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes...
December 2018: Bulletin of Experimental Biology and Medicine
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