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Bulletin of Experimental Biology and Medicine

S A Antonov, E S Manuilova, O V Dolotov, A G Kobylyansky, D R Safina, I A Grivennikov
The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of neurons, but not neuronal progenitors from embryonic stem cells and affected the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. These findings open vista to employ NGF for generation of specific neuron types from embryonic stem cells for fundamental and toxicological studies...
March 31, 2017: Bulletin of Experimental Biology and Medicine
E O Kudasova, L F Vlasova, D E Semenov, E L Lushnikova
Morphological analysis of the subcutaneous fat was performed in rats after subcutaneous implantation of basic dental plastic materials with different hydrophobic and hydrophilic properties. It was shown that subcutaneous implantation of dental plastics with mostly hydrophobic surface and low biocompatibility induced destructive and inflammatory processes of various intensities, sometimes with allergic component; morphological signs of processes persisted for 6 weeks. Modification of basic plastics using glow-discharge plasma and enhancement of their hydrophilicity and biocompatibility significantly reduced the intensity of destructive and inflammatory processes and ensured more rapid (in 2 weeks) repair of the destroyed tissues with the formation of fibrous capsule around the implant...
March 31, 2017: Bulletin of Experimental Biology and Medicine
N V Nizyaeva, T V Sukhacheva, G V Kulikova, M N Nagovitsyna, R A Poltavtseva, N E Kan, V L Tyutyunnik, S V Pavlovich, R A Serov, A I Shchyogolev
Telocytes of placental villi were studied by electronic microscopy during physiological pregnancy. Ultrastructural features of telocytes indicating their heterogeneity and presence of at least three types of villi depending on their localization and kind were observed. All placental telocytes were characterized by small amount of organelles including mitochondria. Presence of long thin processes, which generated a branching network by contacting with each other, served as a typical feature of telocytes including telocytes of the stroma and intermediate villi...
March 31, 2017: Bulletin of Experimental Biology and Medicine
E S Shurkhina, T Yu Polyanskaya, V Yu Zorenko, V M Nesterenko
We examined HCV+ and HCV- hemophilia A patients with knee arthropathy and hematocrit above 38.5%. The mean density of erythrocytes was studied by the phthalate method, intraoperative blood loss was assessed gravimetrically. The volume of blood loss in HCV+ patients with manifest adhesive process and chronic synovitis varied from 300 to 1900 ml, in patients with moderate adhesive process from 400 to 1500 ml. The volume of blood loss in HCV- patients was 300-800 ml. A positive correlation between the blood loss volume and mean density of erythrocytes was detected...
March 31, 2017: Bulletin of Experimental Biology and Medicine
E V Kuevda, E A Gubareva, I S Gumenyuk, A S Sotnichenko, I V Gilevich, R Z Nakokhov, T V Rusinova, T G Yudina, A N Red'ko, S N Alekseenko
We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.
March 31, 2017: Bulletin of Experimental Biology and Medicine
O V Kazakov, A V Kabakov, A F Poveshchenko, I Yu Ishchenko, O V Poveshchenko, D N Strunkin, T V Raiter, S V Michurina, V I Konenkov
Morphological changes in the thymus of female Wistar rats with experimental mammary gland carcinomas were studied. After adjuvant therapy, the area of the cortical matter and density of parenchymal cells in the thymus decreased, while areas of the medulla, connective tissue, and content of immunoblasts and macrophages increased. In the thymuses of rats receiving exogenous DNA, morphological signs of activation of the lymphoid and epithelial components were found: areas of the cortex and medulla, glandular and connective tissue corresponded to the values in intact animals, the counts of lymphocytes in the central part of the cortical matter and of macrophages in all zones of the thymus increased, and lymphocyte migration from the thymus increased (in comparison with the chemotherapy group)...
March 31, 2017: Bulletin of Experimental Biology and Medicine
V N Olesova, M A Amkhadova, T G Simakova, M Z Mirgazizov, M M Pozharitskaya
For evaluation of the efficiency of bone substitute, nanostructurized Gamalant-paste-FORTEPlus was placed into a mandibular defect in rats. Bone tissue reparation was evaluated after 30 days by histological methods under a microscope. Use of bone substitute in experimental mandibular defect ensured more complete and rapid restructuring of the bone tissue in comparison with the control (natural healing).
March 31, 2017: Bulletin of Experimental Biology and Medicine
A E Kotsyuba, V M Chertok, A G Chertok
First-fourth order pial branches of the median cerebral artery were studied by biomicroscopy in male Wistar rats aged 1 and 12 months. Irrespective of age, CO-mediated mechanisms are involved in the regulation of the basal tone of pial vessels of various diameters (more so of arteries with well-developed muscular tunic). Injection of hemin confirmed that endogenous production of CO maintained vasodilatation and this effect was most pronounced in large pial branches of young animals, while zinc protoporphyrin IX blocked this effect in all cases...
March 31, 2017: Bulletin of Experimental Biology and Medicine
A A Spasov, A F Kucheryavenko, V S Sirotenko, K A Gaidukova, A S Morkovnik, V A Anisimova, L N Divaeva, T A Kuz'menko
Antithrombotic activity of a new orally administered antiplatelet compound DAB-15 was compared to that of acetylsalicylic acid, ticlopidine, and clopidogrel in the experimental model of arterial thrombosis in rats caused by surface application of 50% ferric chloride (III) on the carotid artery. Compound DAB-15 exerted a dose-dependent antithrombotic effect and was superior to acetylsalicylic acid, ticlopidine and clopidogrel by 5, 7, and 4.9 times, respectively (by ED50). This necessitates studying of the action mechanism of this antiplatelet compound with consideration of its influence on different stages of the pathogenesis of platelet aggregation...
March 31, 2017: Bulletin of Experimental Biology and Medicine
L G Kolik, A V Nadorova, V N Stolyaruk, I A Miroshkina, I B Tsorin, S A Kryzhanovskii
Effect of trimetazidine (20 and 30 mg/kg) on elevated plus maze behavior of rodents was assessed in the genetic and pharmacological anxiety models. Single intraperitoneal injection of trimetazidine in a dose of 20 mg/kg prevented anxiety development in highly emotional male BALB/c mice and increased the time spent in open arms of the maze. In outbred male rats receiving 10% ethanol solution for 20 weeks, trimetazidine administered intraperitoneally in a dose of 20 mg/kg for 28 days abolished ethanol withdrawal-induced anxiogenesis developed against the background of 4-week alcohol deprivation: it increased the time spent in open arms, the number of entries into open arms, and total locomotor activity in the maze...
March 31, 2017: Bulletin of Experimental Biology and Medicine
O A Bulaeva, P A Abramicheva, T A Balakina, O V Smirnova
We studied possible involvement of prolactin in the regulation of bicarbonate biodynamics using female rat model of cholestasis of pregnancy induced by transplantation of the donor pituitary under the renal capsule of a recipient (hyperprolactinemia) and bile duct ligation (cholestasis). The concentration of bicarbonates in the bile and blood, their excretion, clearance, and reabsorption, as well as glomerular filtration rate and excretion of sodium ions were assessed. It was found that the main effect of prolactin was directed to the kidney-regulated pool of bicarbonates and consisted in stimulation of their clearance and inhibition of reabsorption, which led to a decrease in bicarbonate blood concentration...
March 31, 2017: Bulletin of Experimental Biology and Medicine
I V Gilevich, T V Fedorenko, I A Pashkova, V A Porkhanov, V P Chekhonin
We studied mobilization of bone marrow stem cells the under conditions of boost-therapy with granulocytic CSF and erythropoietin. Immunophenotyping of the peripheral blood was carried out in patients after transplantation of tissue-engineered trachea during the perioperative period. We observed an increase in hemopoietic stem cell pool and appearance of multipotent mesenchymal stromal cells in systemic circulation, their maximum concentration being recorded on days 10-14 after surgery. These data indicated a positive effect of growth factors on mobilization of stem cells...
March 31, 2017: Bulletin of Experimental Biology and Medicine
V I Dubrovina, S V Balakhonov, V V Voitkova, S A Vityazeva, T P Starovoitova, K M Korytov, G F Prozorova, G P Aleksandrova, S I Kolesnikov
The effects of nanostructurized composites argentogalactomannan and argento-poly-1-vinyl-1,2,4-triasole on the kinetics of T cell maturation in the thymus of albino mice were studied. These nanocomposites stimulated the formation of CD3(+)CD4(+)CD8(-) and CD3(+)CD4(-)CD8(+) cells from CD3(-)CD4(-)CD8(-) cells, the effect of argentogalactomannan was more potent. The efficiency of these compounds as agents improving the defense potential deserves special studies.
March 31, 2017: Bulletin of Experimental Biology and Medicine
L I Kozlova, D D Bezmaternykh, M E Mel'nikov, A A Savelov, E D Petrovskii, M B Shtark
Brain EEG-fMRI activity was studied in subjects, who had successfully completed the EEG alpha stimulating training course (20 sessions): for 14 healthy men (20-35 years) three records were obtained in the feedback loop (biofeedback with EEG alpha rhythm with sound reinforcement): in the beginning, middle and at the end of the course. During alpha training, increased functional connectivity was revealed between precuneus network and anterior salience network, left executive control network, default mode network, primary visual network; anterior salience network and executive control network, visual-spatial network...
March 31, 2017: Bulletin of Experimental Biology and Medicine
V M Karimova, K B Pustovit, D V Abramochkin, V S Kuz'min
We studied the effect of extracellular purine nucleotides (NAD(+) and ATP) on spontaneous arrhythmogenic activity caused by norepinephrine in myocardial sleeves of pulmonary veins. In pulmonary veins, NAD(+) and ATP reduced the frequency of action potentials and their duration at regular type of spontaneous activity caused by norepinephrine. NAD(+) and ATP lengthened the intervals between spike bursts at periodic (burst) type of spontaneous activity. In addition, ATP shortened the duration of spike bursts and the number of action potentials in the "bursts" caused by norepinephrine in the pulmonary veins...
March 30, 2017: Bulletin of Experimental Biology and Medicine
N S Fattakhov, D A Skuratovskaya, M A Vasilenko, E V Kirienkova, P A Zatolokin, N I Mironyuk, L S Litvinova
We studied association of single nucleotide polymorphism Glu298Asp (rs1799983) of the NOS3 gene with the risk of metabolic syndrome in the Slavic population. Blood samples were obtained from 128 patients with metabolic syndrome and 100 healthy individuals. Polymorphism Glu298Asp of the NOS3 gene was genotyped by allele-specific PCR. Allele Asp (OR=1.95, 95%CI 1.29-2.95, p=0.007) and genotype Asp/Asp (OR=2.56, 95%CI 0.98-6.72, p=0.04) were associated with the risk of metabolic syndrome in Slavic population. Patients with metabolic syndrome carrying genotype Asp/Asp had higher serum endothelin-1 level in comparison with Glu/Asp and Glu/Glu carriers...
March 30, 2017: Bulletin of Experimental Biology and Medicine
N V Kirbaeva, N E Sharanova, A V Vasil'ev, S S Pertsov
We studied proteome profile of blood serum of Wistar rats with different behavioral activity immediately and in 1 and 3 days after acute stress on the model of 12-h immobilization during the nighttime. Comparative analysis of 2D-electrophoretograms revealed differences in the expression of serum proteins in non-stressed (control) and stressed (experimental) rats. We found 22 protein spots that characterized the proteomic features of blood serum in rats with different prognostic resistance to stress. Mass-spectrometry of isolated spots identified 6 functional proteins...
March 30, 2017: Bulletin of Experimental Biology and Medicine
T V Kozyreva, E S Meyta, V P Kozaruk
We studied the role of purinergic P2X receptors in the body response to cooling. In experiments on rats, P2X receptor antagonist PPADS was administered in different modes, which resulted in changes of different characteristics of the thermoregulatory response to cold. Iontophoresis of P2X antagonist into the skin decreased the thermal thresholds of all thermoregulatory responses to cooling, which can attest to a modulating effect of P2X receptors on peripheral thermosensitive afferents. Intraperitoneal administration of P2X antagonist suppressed thermoregulatory activity of skeletal muscles (shivering) developing during cooling without changing the thresholds of thermoregulatory responses...
March 30, 2017: Bulletin of Experimental Biology and Medicine
Yu O Fedotova, V K Akulova, S G Pivina
Characteristics of depressive-like behavior of male rats with androgen deficiency born by mothers subjected to prenatal stress during pregnancy were assessed by using Porsolt tests and open-field tests. The level of depression-like behavior in prenatally stressed males increased more intensively than in non-stressed gonadectomized males. Chronic administration of testosterone propionate (0.5 mg/kg, intramuscularly, for 14 days) increased depressive behavior in prenatally stressed gonadectomized males in contrast to its antidepressant effect in nonstressed gonadectomized rats...
March 30, 2017: Bulletin of Experimental Biology and Medicine
S G Kalinichenko, N Yu Matveeva, R E Kostiv, A V Puz'
The study established enhanced expression of vascular endothelial growth factor (VEGF) in the subpopulation of osteoblasts located in the regeneration region of femoral bone fracture near the titanium implants with bioactive calcium phosphate and hydroxyapatite coatings and suppressed activity of transforming growth factor-β2 (TGF-β2) in chondroblasts during the two weeks after surgery. In the delayed posttraumatic period, the distribution of TGF-β2 inversely related to its maximal activity. The data revealed the up-regulating effect of bioresorbable coatings on expression of VEGF and TGF-β2 and their implication in the control over various stages of reparative osteogenesis...
March 30, 2017: Bulletin of Experimental Biology and Medicine
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