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Biochemical and Biophysical Research Communications

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https://www.readbyqxmd.com/read/29792866/cardiac-extracellular-matrix-hydrogel-together-with-or-without-inducer-cocktail-improves-human-adipose-tissue-derived-stem-cells-differentiation-into-cardiomyocyte-like-cells
#1
Masoumeh Baghalishahi, Seyed Hasan Efthekhar-Vaghefi, Abbas Piryaei, S N Nematolahi-Mahani, Hamid Reza Mollaei, Yousef Sadeghi
Studies have demonstrated that differentiation of stem cells into cardiomyocytes is a complex phenomenon that requires sufficient inducing factors at various time points. Cardiac extracellular matrix (cECM) could provide tissue specific microenvironment and act as an inductive template for efficient cell differentiation. The aim of this study was to investigate the effect of cECM on differentiation of human adipose tissue-derived stem cells (hADSCs) into cardiomyocytes using cECM hydrogel in combination with a cardiac inductive cocktail...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792865/aspirin-restores-abt-737-mediated-apoptosis-in-human-renal-carcinoma-cells
#2
Yen-Chuan Ou, Jian-Ri Li, Jiaan-Der Wang, Wen-Ying Chen, Yu-Hsiang Kuan, Ching-Ping Yang, Su-Lan Liao, Hsi-Chi Lu, Chun-Jung Chen
Aspirin is a novel chemopreventive agent against malignancy. However, outcomes of aspirin monotherapy of renal cell carcinoma (RCC) are inconsistent across studies. ABT-737, an BH3 mimetic inhibitor, is also a promising antitumor drug. Cancer cells including those from RCC, that have high levels of Mcl-1, are refractory to ABT-737-induced apoptosis. We here investigated how aspirin treatment modulates the ABT-737-induced apoptosis. Using the in vitro model of human 786-O cells, we showed that aspirin had sensitized cells to ABT-737 induced apoptosis...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792864/delavatine-a-an-unusual-isoquinoline-alkaloid-exerts-anti-inflammation-on-lps-induced-proinflammatory-cytokines-production-by-suppressing-nf-%C3%AE%C2%BAb-activation-in-bv-2-microglia
#3
Qing Xie, Guo-Zhen Wu, Niao Yang, Yun-Heng Shen, Jian Tang, Wei-Dong Zhang
Delavatine A, an unusual isoquinoline alkaloid isolated from I. delavayi, was first studied for anti-inflammatory effect using lipopolysaccharide (LPS)-induced BV-2 microglia. In the present study, we found that delavatine A substantially suppressed the LPS-induced pro-inflammatory mediators, nitric oxide (NO), and tumor necrosis factor-a (TNF-a), interleukin-6 (IL-6), interleukin-1β (IL-1β) in BV-2 microglial cells. These effects resulted from the inhibition of their regulatory genes inducible NO synthase (iNOS), cycloxygenase-2 (COX-2) and TNF-a, IL-6, IL-1β...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792863/identification-of-immunodominant-cd8-epitope-in-the-stalk-domain-of-influenza-b-viral-hemagglutinin
#4
Abenaya Muralidharan, Caroline Gravel, Amparo Duran, Louise Larocque, Changgui Li, Adrian Zetner, Gary Van Domselaar, Lisheng Wang, Xuguang Li
Human infections by type B influenza virus constitute about 25% of all influenza cases. The viral hemagglutinin is comprised of two subunits, HA1 and HA2. While HA1 is constantly evolving in an unpredictable fashion, the HA2 subunit is highly conserved, making it a potential candidate for a universal vaccine. However, immunodominant epitopes in the HA2 subunit remain largely unknown. To delineate MHC Class I epitopes, we first identified 9-mer H-2Kd -restricted CD8 T cell epitopes in the HA2 domain by in silico analyses, followed by evaluating the immunodominance of these peptides in mice challenged with the virus...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792862/the-reason-for-the-low-ca-2-sensitivity-of-thin-filaments-associated-with-the-glu41lys-mutation-in-the-tpm2-gene-is-freezing-of-tropomyosin-near-the-outer-domain-of-actin-and-inhibition-of-actin-monomer-switching-off-during-the-atpase-cycle
#5
Stanislava V Avrova, Olga E Karpicheva, Nikita A Rysev, Armen O Simonyan, Vladimir V Sirenko, Charles S Redwood, Yurii S Borovikov
The E41K mutation in TPM2 gene encoding muscle regulatory protein beta-tropomyosin is associated with nemaline myopathy and cap disease. The mutation results in a reduced Ca2+ -sensitivity of the thin filaments and in muscle weakness. To elucidate the structural basis of the reduced Ca2+ -sensitivity of the thin filaments, we studied multistep changes in spatial arrangement of tropomyosin (Tpm), actin and myosin heads during the ATPase cycle in reconstituted fibers, using the polarized fluorescence microscopy...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792861/nlgn3-protects-retinal-cells-from-h-2-o-2-via-activating-nrf2-signaling
#6
Xiu-Miao Li, Dan Huang, Qing Yu, Jin Yao, Jian Yang
Intensified oxidative stress can cause severe damage to human retinal pigment epithelium (RPE) cells and retinal ganglion cells (RGCs). The potential effect of neuroligin-3 (NLGN3) against the process is studied here. Our results show that NLGN3 efficiently inhibited hydrogen peroxide (H2 O2 )-induced death and apoptosis in human RPE cells and RGCs. H2 O2 -induced reactive oxygen species (ROS) production, lipid peroxidation and DNA damage in retinal cells were alleviated by NLGN3. NLGN3 activated nuclear-factor-E2-related factor 2 (Nrf2) signaling, enabling Nrf2 protein stabilization, nuclear translocation and expression of key anti-oxidant enzymes (HO1, NOQ1 and GCLC) in RPE cells and RGCs...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792860/diphenyleneiodonium-enhances-oxidative-stress-and-inhibits-japanese-encephalitis-virus-induced-autophagy-and-er-stress-pathways
#7
Manish Sharma, Kiran Bala Sharma, Shailendra Chauhan, Sankar Bhattacharyya, Sudhanshu Vrati, Manjula Kalia
Diphenyleneiodonium (DPI) and N-acetyl-L-cysteine (NAC), two widely used anti-oxidants, were employed to evaluate the role of oxidative stress in Japanese encephalitis virus (JEV) induced autophagy, stress responses and replication. DPI and NAC exerted opposite effects on ROS levels in JEV infected mouse neuronal cells (Neuro2a), mouse embryonic fibroblasts (MEFs) and human epithelial cells (HeLa). While NAC effectively quenched ROS, DPI enhanced ROS levels, suggesting that DPI induces oxidative stress in JEV infected cells...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792859/long-non-coding-rna-miat-is-estrogen-responsive-and-promotes-estrogen-induced-proliferation-in-er-positive-breast-cancer-cells
#8
Yuehua Li, Baohong Jiang, Xiaoping Wu, Qin Huang, Wenqi Chen, Hongbo Zhu, Xiaofei Qu, Liming Xie, Xin Ma, Guo Huang
Estrogen drives the development and progression of estrogen receptor (ER)-positive breast cancer. However, the detailed mechanism underlying ER-driven carcinogenesis remains unclear despite extensive studies. Previously reports indicated higher expression of long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) in ER-positive breast cancer tissues than in ER-negative tissues. However, the functional relevance of MIAT in ER-positive breast cancer tumorigenesis was poorly understood...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792858/luciferase-of-the-japanese-syllid-polychaete-odontosyllis-umdecimdonta
#9
Darrin T Schultz, Alexey A Kotlobay, Rustam Ziganshin, Artyom Bannikov, Nadezhda M Markina, Tatiana V Chepurnyh, Ekaterina S Shakhova, Ksenia Palkina, Steven H D Haddock, Ilia V Yampolsky, Yuichi Oba
Odontosyllis undecimdonta is a marine syllid polychaete that produces bright internal and exuded bioluminescence. Despite over fifty years of biochemical investigation into Odontosyllis bioluminescence, the light-emitting small molecule substrate and catalyzing luciferase protein have remained a mystery. Here we describe the discovery of a bioluminescent protein fraction from O. undecimdonta, the identification of the luciferase using peptide and RNA sequencing, and the in vitro reconstruction of the bioluminescence reaction using highly purified O...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792857/tgf-%C3%AE-plays-a-vital-role-in-triple-negative-breast-cancer-tnbc-drug-resistance-through-regulating-stemness-emt-and-apoptosis
#10
Xiaodan Xu, Lu Zhang, Xiaogang He, Ping Zhang, Caihong Sun, Xiaojun Xu, Yaojuan Lu, Feifei Li
Triple negative breast cancer (TNBC) is the most malignant subtype of breast cancer in which the cell surface lacks usual targets for drug to exhibit its effects. Epirubicin (Epi) is widely used for TNBC, but a substantial number of patients develop Epi resistance that is usually associated with poor prognosis. Transforming growth factor (TGF-β) is a multifunctional cytokine. In recent study, it appears that TGF-β influences the cancer stem cell population, thus, the drug resistance of cancer may also be affected...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787763/the-torc1-signaling-pathway-regulates-respiration-induced-mitophagy-in-yeast
#11
Yang Liu, Koji Okamoto
Mitophagy is an evolutionarily conserved autophagy process that selectively degrades mitochondria. This catabolic event is considered to be a mitochondrial quality control system crucial for cell homeostasis, however, mechanisms regulating mitophagy remain largely unknown. Here we show that the TORC1 (target of rapamycin complex 1) signaling pathway regulates mitophagy in budding yeast via SEACIT (Seh1-associated complex inhibiting TORC1) consisting of Iml1, Npr2, and Npr3. Cells lacking SEACIT displayed significant reductions in mitophagy during prolonged respiratory growth, while the other selective autophagy processes are less affected...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787762/hypoxic-glioblastoma-release-exosomal-vegf-a-induce-the-permeability-of-blood-brain-barrier
#12
Chen Zhao, Hongyan Wang, Chenghao Xiong, Yu Liu
Exosomes are nano-vesicles released by tumor cells to modulate extracellular environment. Accumulating evidence revealed that glioblastoma derived exosomes contain multiple pro-angiogenic factors to induce the proliferation of endothelial cells. Here, we investigated the role of GBM-derived exosomes in inducing the permeability of the blood-brain barrier. We found that VEGF-A was over-expressed in hypoxic GBM-derived exosomes, which enhance the permeability of a BBB in vitro model by interrupting the expression of claudin-5 and occludin...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787761/biophysical-studies-and-modelling-indicate-the-binding-preference-of-taz-ww-domain-for-lats1-ppxy-motif
#13
Apoorva Verma, Fu Lin, Tan Ying Chong, Muhammad Nur Hidayat, Chacko Jobichen, Hao Fan, J Sivaraman
The Hippo tumor suppressor pathway is an important regulator of cell proliferation and apoptosis, and signal transduction occurs through phosphorylation of the effector protein TAZ by the serine/threonine kinase LATS1/2. Here, we report the biophysical and computational studies to characterize the interaction between TAZ and LATS1/2 through WW domain-PPxY motif binding. We show that the TAZ WW domain exhibits a binding preference for the second of the two PPxY motifs of LATS1 in vitro. We modelled the structure of the domain in complex with LATS1 PPxY2 peptide and, through molecular dynamics simulations, show that WW domain-PPxY2 complex is stable with some flexibility in the peptide region...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787760/in-vivo-gene-correction-with-targeted-sequence-substitution-through-microhomology-mediated-end-joining
#14
Jeong Hong Shin, Soobin Jung, Suresh Ramakrishna, Hyongbum Henry Kim, Junwon Lee
Genome editing technology using programmable nucleases has rapidly evolved in recent years. The primary mechanism to achieve precise integration of a transgene is mainly based on homology-directed repair (HDR). However, an HDR-based genome-editing approach is less efficient than non-homologous end-joining (NHEJ). Recently, a microhomology-mediated end-joining (MMEJ)-based transgene integration approach was developed, showing feasibility both in vitro and in vivo. We expanded this method to achieve targeted sequence substitution (TSS) of mutated sequences with normal sequences using double-guide RNAs (gRNAs), and a donor template flanking the microhomologies and target sequence of the gRNAs in vitro and in vivo...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787759/glycosylation-status-of-nicastrin-influences-catalytic-activity-and-substrate-preference-of-%C3%AE-secretase
#15
Mohammad Moniruzzaman, Seiko Ishihara, Mika Nobuhara, Hidekazu Higashide, Satoru Funamoto
γ-Secretase complex, the assembly of nicastrin (NCT), Presenilin (PS), Presenilin Enhancer-2 (PEN-2) and Anterior pharynx defective 1 (Aph-1), catalyzes the cleavage of amyloid precursor protein to generate amyloid-β protein (Aβ), the main culprit of Alzheimer's disease. NCT becomes matured through complex glycosylation and play important role in γ-secretase activity by interacting with catalytic subunit PS. However, the role of NCT glycosylation on γ-secretase activity and substrate specificity is still unknown...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787758/tissue-factor-mediates-microvesicles-shedding-from-mda-mb-231-breast-cancer-cells
#16
Araci M da R Rondon, Vitor H de Almeida, Tainá Gomes, Bruno R F Verçoza, Renato S Carvalho, Sandra König, Juliany C F Rodrigues, Claudia Dos S Mermelstein, Henri H Versteeg, Robson Q Monteiro
Extracellular vesicles, such as microvesicles (MVs), were identified as important players in tumor progression and acquisition of an aggressive phenotype. Tissue factor (TF) is a transmembrane protein that initiates the blood coagulation cascade. In tumor cells, TF has been associated with aggressiveness and cancer progression. Previous studies demonstrate that TF is incorporated into MVs secreted by tumor cells; however, it is unknown whether TF is actively involved in the release of MVs. Here, we investigated the influence of TF expression on the release of MVs...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787757/dlx2-overexpression-enhanced-accumulation-of-type-ii-collagen-and-aggrecan-by-inhibiting-mmp13-expression-in-mice-chondrocytes
#17
Jianfei Zhang, Wenbin Zhang, Jun Shi, Jiewen Dai, Steve Guofang Shen
Genetic studies revealed a crucial role of Distal-homebox (Dlx) genes in skeletal development, and our previous study demonstrated overexpressing Dlx2 in neural crest cells led to abnormal cartilage structure, including ectopic cartilage in the maxillary region and nasal bone in mice. The aim of this study was to investigate how Dlx2 overexpression affects chondrogenesis in mouse chondroblast cell line TMC23 and the underlying mechanism. We first demonstrated that Dlx2 expression was upregulated during chondrogenesis in TMC23 cells...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787756/fibronectin-type-iii-domain-containing-4-attenuates-hyperlipidemia-induced-insulin-resistance-via-suppression-of-inflammation-and-er-stress-through-ho-1-expression-in-adipocytes
#18
Wonjae Lee, Subin Yun, Geum Hee Choi, Tae Woo Jung
Although Fibronectin Type III Domain Containing 4 (FNDC4) has been reported to be involved in the modulation of inflammation in macrophages, its effects on inflammation and insulin resistance in adipose tissue are unknown. In the current study, we investigated the effects of FNDC4 on hyperlipidemia-mediated endoplasmic reticulum (ER) stress, inflammation, and insulin resistance in adipocytes via the AMP-activated protein kinase (AMPK)/heme oxygenase-1 (HO-1)-mediated pathway. Hyperlipidemia-induced nuclear factor κB (NFκB), inhibitory κBα (IκBα) phosphorylation, and pro-inflammatory cytokines such as TNFα and MCP-1 were markedly mitigated by FNDC4...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787755/vibsanin-a-sensitizes-human-acute-myeloid-leukemia-cells-to-tyrosine-kinase-inhibitor-induced-myeloid-differentiation-via-activation-of-pkc-and-upregulation-of-lyn
#19
Xing Shen, Shuang Xing, Lu Zhang, Fangmin Wang, Hongling Ou, Yajun Shan, He Xiao, Guolin Xiong, Xinru Wang, Qinshi Zhao, Yuwen Cong, Zuyin Yu
Differentiation therapies have been proposed to overcome the impaired cell differentiation in acute myeloid leukemia (AML). However, thus far the all-trans retinoic acid-based differentiation therapy has been the only successful modality in treating acute promyelocytic leukemia. Here, we showed that vibsanin A, a novel protein kinase C (PKC) activator, sensitized AML cells to tyrosine kinase inhibitor (TKI)-induced differentiation. Vibsanin A augmented the ability of TKIs to induce growth inhibition and G1 cell cycle arrest of AML cells...
May 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29787754/different-players-generate-positive-responses-in-two-in-vitro-cytokine-assay-formats-with-aqueous-and-immobilized-tgn1412-analog
#20
Yoshika Iwata, Asako Harada, Chiyomi Kubo, Tomoaki Inoue, Mitsuyasu Tabo, Masayuki Mishima
To detect potential risk of severe cytokine release syndrome, in vitro assay formats with human cells have been developed. The two major testing platforms are a combination of whole blood with aqueous-phase test articles (whole blood cytokine assay, WBCA) and peripheral blood mononuclear cells with solid-phase articles (PBMC assay). Significant induction of cytokines was seen in both assays after treatment with a widely used control agent, TGN1412 or its analog CD28SA, but the WBCA cytokine profile differed from what was expected from clinical experience...
May 19, 2018: Biochemical and Biophysical Research Communications
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