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Biochemical and Biophysical Research Communications

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https://www.readbyqxmd.com/read/29146190/newly-developed-selective-immunoinactivation-assay-revealed-reduction-in-adipose-triglyceride-lipase-activity-in-peripheral-leucocytes-from-patients-with-idiopathic-triglyceride-deposit-cardiomyovasculopathy
#1
Atsuko Takagi, Yasuyuki Ikeda, Kunihisa Kobayashi, Kazuhiro Kobayashi, Yoshihiko Ikeda, Junji Kozawa, Hideyuki Miyauchi, Ming Li, Chikako Hashimoto, Yasuhiro Hara, Satoshi Yamaguchi, Akira Suzuki, Tatsushi Toda, Hironori Nagasaka, Ken-Ichi Hirano
Triglyceride deposit cardiomyovasculopathy (TGCV) is a rare and newly identified disease among patients requiring cardiac transplantation. TGCV is characterized by cardiomyocyte steatosis and triglyceride (TG)-deposit atherosclerosis, resulting from the abnormal intracellular metabolism of TG. TGCV is classified into primary and idiopathic types. Primary TGCV carries ultra-rare genetic mutations in the adipose triglyceride lipase (ATGL), a rate-liming enzyme that hydrolyzes intracellular TG in adipose and non-adipose tissues...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146189/oxidized-low-density-lipoprotein-promotes-osteoclast-differentiation-from-cd68-positive-mononuclear-cells-by-regulating-hmgb1-release
#2
Chunfeng Hou, Luan Luan, Chunfeng Ren
Patients with ankylosing spondylitis (AS) have an increased risk for cardiovascular mortality. The circulating ox-LDL/LDL ratio is associated with subclinical atherosclerosis in patients with systemic lupus erythematosus. In this study, we found that the ox-LDL/LDL ratio was increased in AS patients. The levels of serum RANKL and HMGB1 were also elevated in AS patients, and the number of CD68(+)/RANK(+) cells was increased in peripheral blood from AS patients. 0.03% ox-LDL in LDL, similar to the ox-LDL/LDL ratio in peripheral blood from AS patients, promoted cytoplasmic translocation and release of HMGB1 as well as RANK expression...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146188/old-drug-new-tricks-chlorhexidine-acts-as-a-potential-allosteric-inhibitor-toward-pak1
#3
Han-Wei Huang, Xiang-Yu Zhang, Pei-Lu Song, Hai-Lun Jiang, Wei Li, Peng-Liang Wang, Jian Wang, Fu-Nan Liu
This paper describes the identification of chlorhexidine, an agent commonly used in clinical as a novel potential allosteric inhibitor of PAK1. In cellular assays, chlorhexidine showed a good inhibitory profile, and its inhibitory profile was even better than IPA-3, a well-known allosteric inhibitor. In pharmacology experiments, chlorhexidine successfully inhibited the relief of PAK1 dimer and inhibited the activation of PAK1. Our findings offer an insight for the new drug development of PAK1 inhibitor. We also provide a possible explanation for the phenomenon that the application of the chlorhexidine in peritoneal lavage inhibited the development of tumor...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146187/lox-related-collagen-crosslink-changes-act-as-an-initiator-of-bone-fragility-in-a-zdf-rats-model
#4
Xun Xiao, Jie Ren, Jun Chen, Zhaohui Liu, Ye Tian, Neel R Nabar, Min Wang, Liang Hao
Diabetes mellitus type 2 (DM2) results in bone abnormalities that manifest as increased bone fragility. Bone consists of two phases, the mineral phase and the matrix phase, and disorders in both are seen in DM2. However, the phase in which DM2 mediated bone fragility is initiated is still unknown. In this study, a male Zucker diabetic fatty (fa/fa) (ZDF) rat model was used to investigate the underlying mechanism initiating DM2 mediated bone fragility. The fracture surface morphology, pre- and post-yield bone mechanical behavior, insoluble collagen volume, lysyl oxidase family (LOX) enzyme levels and correlation analysis was performed to determine the relationship between insoluble collagen and post-yield behavior...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146186/human-soluble-thrombomodulin-induced-blockade-of-peripheral-hmgb1-dependent-allodynia-in-mice-requires-both-the-lectin-like-and-egf-like-domains
#5
Yusuke Hayashi, Ryuichi Tsujita, Maho Tsubota, Haruka Saeki, Fumiko Sekiguchi, Goichi Honda, Atsufumi Kawabata
Thrombomodulin (TM), an endothelial protein with anti-coagulant activity, is composed of 5 domains, D1-D5. Recombinant human soluble TM (TMα) consisting of D1-D3, which is generated in CHO cells, suppresses inflammatory and nociceptive signals by inactivating high mobility group box 1 (HMGB1), one of damage-associated molecular patterns. TMα sequesters HMGB1 with the lectin-like D1 and promotes its degradation by thrombin binding to the EGF-like D2. We prepared TM's D123, D1 and D2 by the protein expression system of yeast, and evaluated their effects on HMGB1 degradation in vitro and on the allodynia caused by HMGB1 in distinct redox forms in mice in vivo...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146185/biomarker-identification-for-statin-sensitivity-of-cancer-cell-lines
#6
Vineet K Raghu, Colin H Beckwitt, Katsuhiko Warita, Alan Wells, Panayiotis V Benos, Zoltán N Oltvai
Statins are potent cholesterol reducing drugs that have been shown to reduce tumor cell proliferation in vitro and tumor growth in animal models. Moreover, retrospective human cohort studies demonstrated decreased cancer-specific mortality in patients taking statins. We previously implicated membrane E-cadherin expression as both a marker and mechanism for resistance to atorvastatin-mediated growth suppression of cancer cells; however, a transcriptome-profile-based biomarker signature for statin sensitivity has not yet been reported...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146184/glutamate-production-from-ammonia-via-glutamate-dehydrogenase-2-activity-supports-cancer-cell-proliferation-under-glutamine-depletion
#7
Yukiko Takeuchi, Yasumune Nakayama, Eiichiro Fukusaki, Yasuhiro Irino
Cancer cells rapidly consume glutamine as a carbon and nitrogen source to support proliferation, but the cell number continues to increase exponentially after glutamine is nearly depleted from the medium. In contrast, cell proliferation rates are strongly depressed when cells are cultured in glutamine-free medium. How cancer cells survive in response to nutrient limitation and cellular stress remains poorly understood. In addition, rapid glutamine catabolism yields ammonia, which is a potentially toxic metabolite that is secreted into the extracellular space...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146183/d-rhamnose-%C3%AE-hederin-inhibits-migration-and-invasion-of-human-breast-cancer-cell-line-mda-mb-231
#8
Lin Cheng, Tian-Song Xia, Liang Shi, Lingyun Xu, Weixian Chen, Yulan Zhu, Qiang Ding
Many natural products have been shown to have inhibitory effects on the metastatic process of various cancers including breast cancer. An active triterpenoid saponin D Rhamnose β-hederin (DRβ-H) from Clematis ganpiniana, has been known induce the apoptosis of breast cancer cells, but the effect of DRβ-H on the metastasis of breast cancer cells is largely unknown. In this study, we demonstrated that a non-cytotoxic concentration of DRβ-H markedly suppressed wound healing migration, migration through the chamber and invasion through the matrigel...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146182/plakophilin3-increases-desmosome-assembly-size-and-stability-by-increasing-expression-of-desmocollin2
#9
Mansa Gurjar, Kumarkrishna Raychaudhuri, Snehal Mahadik, Divya Reddy, Apurva Atak, Trupti Shetty, Kruthi Rao, Mansi S Karkhanis, Prajakta Gosavi, Lalit Sehgal, Sanjay Gupta, Sorab N Dalal
Previous reports show that the desmosomal plaque protein plakophilin3 (PKP3) is essential for desmosome formation. Here, we report that PKP3 over-expression decreases calcium dependency for de novo desmosome formation and makes existing cell-cell adhesion junctions more resilient in low calcium medium due to an increase in desmocollin2 expression. PKP3 overexpression increases the stability of other desmosomal proteins independently of the increase in DSC2 levels and regulates desmosome formation and stability by a multimodal mechanism affecting transcription, protein stability and cell border localization of desmosomal proteins...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29146181/proximity-labeling-of-cis-ligands-of-cd22-siglec-2-reveals-stepwise-%C3%AE-2-6-sialic-acid-dependent-and-independent-interactions
#10
Amin Alborzian Deh Sheikh, Chizuru Akatsu, Akihiro Imamura, Hajjaj H M Abdu-Allah, Hiromu Takematsu, Hiromune Ando, Hideharu Ishida, Takeshi Tsubata
Lectins expressed on the cell surface are often bound and regulated by the membrane molecules containing the glycan ligands on the same cell (cis-ligands). However, molecular nature and function of cis-ligands are generally poorly understood partly because of weak interaction between lectins and glycan ligands. Cis-ligands are most extensively studied in CD22 (also known as Siglec-2), an inhibitory B lymphocyte receptor specifically recognizing α2,6 sialic acids. CD22, CD45 and IgM are suggested to be ligands of CD22...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137987/degree-of-pectin-methyl-esterification-in-endosperm-cell-walls-is-involved-in-embryo-bending-in-arabidopsis-thaliana
#11
José E Cruz-Valderrama, Karina Jiménez-Durán, Esther Zúñiga-Sánchez, Alexis Salazar-Iribe, Judith Márquez-Guzmán, Alicia Gamboa-deBuen
The endosperm is a transitory structure involved in proper embryo elongation. The cell walls of mature seed endosperm are generally composed of a uniform distribution of cellulose, unesterified homogalacturonans, and arabinans. Recent studies suggest that changes in cell wall properties during endosperm development could be related to embryo growth. The degree of methyl esterification of homogalacturonans may be involved in this endosperm tissue remodelling. The relevance of the degree of homogalacturonan methyl esterification during seed development was determined by immunohistochemical analyses using a panel of probes with specificity for homogalaturonans with different degrees of methyl esterification...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137986/long-range-stress-transmission-guides-endothelial-gap-formation
#12
C Corey Hardin, Joyjit Chattoraj, Greeshma Manomohan, Jader Colombo, Trong Nguyen, Dhananjay Tambe, Jeffrey J Fredberg, Konstantin Birukov, James P Butler, Emanuela Del Gado, Ramaswamy Krishnan
In endothelial gap formation, local tractions exerted by the cell upon its basal adhesions are thought to exceed balancing tensile stresses exerted across the cell-cell junction, thus causing the junction to rupture. To test this idea, we mapped evolving tractions, intercellular stresses, and corresponding growth of paracellular gaps in response to agonist challenge. Contrary to expectation, we found little to no relationship between local tensile stresses and gap formation. Instead, we discovered that intercellular stresses were aligned into striking multi-cellular domains punctuated by defects in stress alignment...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137985/the-effect-of-surface-charge-on-the-thermal-stability-and-ice-recrystallization-inhibition-activity-of-antifreeze-protein-iii-afp-iii
#13
R C Deller, B M Carter, I Zampetakis, F Scarpa, A W Perriman
The aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (-40 °C to +90 °C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137984/recombinant-human-glp-1-rhglp-1-alleviating-renal-tubulointestitial-injury-in-diabetic-stz-induced-rats
#14
Weiqin Yin, Shiqing Xu, Zai Wang, Honglin Liu, Liang Peng, Qing Fang, Tingting Deng, Wenjian Zhang, Jinning Lou
GLP-1-based treatment improves glycemia through stimulation of insulin secretion and inhibition of glucagon secretion. Recently, more and more findings showed that GLP-1 could also protect kidney from diabetic nephropathy. Most of these studies focused on glomeruli, but the effect of GLP-1 on tubulointerstitial and tubule is not clear yet. In this study, we examined the renoprotective effect of recombinant human GLP-1 (rhGLP-1), and investigated the influence of GLP-1 on inflammation and tubulointerstitial injury using diabetic nephropathy rats model of STZ-induced...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137983/transcriptional-regulation-of-acetoacetyl-coa-synthetase-by-sp1-in-neuroblastoma-cells
#15
Shinya Hasegawa, Masahiko Imai, Masahiro Yamasaki, Noriko Takahashi, Tetsuya Fukui
Acetoacetyl-CoA synthetase (AACS) is the enzyme responsible for cholesterol and fatty acid synthesis in the cytosol. We have previously shown that AACS has an important role in normal neuronal development and that knockdown of SREBP-2, which orchestrates cholesterol synthesis, resulted in the downregulation of AACS mRNA levels. In this study, we investigated the transcriptional mechanism of AACS in Neuro-2a, neuroblastoma cells. Luciferase assay showed that the minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137982/adipose-tissue-complement-factor-b-promotes-adipocyte-maturation
#16
Hiroaki Matsunaga, Misaki Iwashita, Takanori Shinjo, Akiko Yamashita, Mitsudai Tsuruta, Shoichiro Nagasaka, Ataru Taniguchi, Mitsuo Fukushima, Naoya Watanabe, Fusanori Nishimura
OBJECTIVES: It is well-known that the complement system plays an essential role in host immunity. Observational studies have indicated that complement system-related molecules such as complement factor B (CfB) and other components are correlated with obesity and/or insulin resistance parameters. In this study, we investigated the role of adipocyte-derived CfB in adipose tissue metabolism. METHODS: We investigated the expression level of complement system-related genes in adipocytes...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137981/umbilical-cord-derived-mesenchymal-stem-cells-alleviated-inflammation-and-inhibited-apoptosis-in-interstitial-cystitis-via-akt-mtor-signaling-pathway
#17
Juncong Xie, Bolong Liu, Jialiang Chen, Yuancheng Xu, Hailun Zhan, Fei Yang, Wenbiao Li, Xiangfu Zhou
Interstitial cystitis (IC) is a bladder syndrome characterized by pelvic pain and urinary frequency without infection or other identifiable pathology. There are no effective treatments to cure IC. This study investigated the effects of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) injection on IC rat model. Furthermore, we used a coculture system to find the possible molecular mechanism on the human uroepithelial cells (SV-HUC-1), which was the cell model of IC. A rat model of IC was established via systemic injection with cyclophosphamide (CYP) and a cell model of IC was induced by being exposed to tumor necrosis factor (TNF)-α (10 ng/ml)...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137980/interaction-of-alpha-synuclein-with-cytogaligin-a-protein-encoded-by-the-proapoptotic-gene-galig
#18
Saïd El Haddad, Amandine Serrano, Thierry Normand, Chloé Robin, Martine Dubois, Fabienne Brule-Morabito, Lucile Mollet, Stéphane Charpentier, Alain Legrand
GALIG, an internal gene to the human galectin-3 gene, encodes two distinct proteins, Mitogaligin and Cytogaligin through translation of a unique mRNA in two overlapping alternative reading frames. When overexpressed GALIG induces apoptosis. In cultured cells, Mitogaligin destabilizes mitochondria membranes through interaction with cardiolipin. Little is known regarding the role of Cytogaligin. This protein displays multiple subcellular localizations; cytosol, nucleus, and mitochondria. We illustrate here that Cytogaligin is also secreted in the extracellular medium...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137979/acute-and-chronic-cadmium-telluride-quantum-dots-exposed-human-bronchial-epithelial-cells-the-effects-of-particle-sizes-on-their-cytotoxicity-and-carcinogenicity
#19
Wei Zheng, Yan-Ming Xu, Dan-Dan Wu, Yue Yao, Zhan-Ling Liang, Heng Wee Tan, Andy T Y Lau
Quantum dots (QDs) are semiconducting nanocrystals with unique optical properties. When coated with shell/capping, QDs are not deleterious to cells and organisms. However, when QDs are retained in the cellular environment for a certain period of time, their coatings may be degraded, yielding "naked" QDs. Although some studies have documented the acute effects of QDs in various cell lines, however, to our knowledge, there are no published studies on the chronic effects of QDs in normal lung cells. In this study, we therefore sought to study the effects of cadmium telluride (CdTe) QDs of various particles sizes on their cytotoxicity and carcinogenicity in normal human bronchial epithelial cells (BEAS-2B)...
November 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29137978/endothelial-robo4-regulates-il-6-production-by-endothelial-cells-and-monocytes-via-a-crosstalk-mechanism-in-inflammation
#20
Keisuke Shirakura, Ryosuke Ishiba, Taito Kashio, Miki Sakai, Yu Fukushima, Nana Yamamoto, Shiori Manabe, Naoya Shigesada, Toru Tanaka, Nobumasa Hino, William C Aird, Takefumi Doi, Yoshiaki Okada
Roundabout4 (Robo4) is an endothelial cell-specific receptor that stabilizes vasculature in pathological angiogenesis. Previous studies have shown that Robo4 is a potential therapeutic target for inflammatory diseases, but its precise roles in inflammation remain unclear. To investigate physiological Robo4 functions in inflammation, we performed a loss-of-function study in vitro and in vivo using lipopolysaccharide (LPS)-induced endotoxemia models. Subcutaneous injection of LPS into Robo4-knockout mice reduced circulating IL-6 levels...
November 11, 2017: Biochemical and Biophysical Research Communications
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