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Biochemical and Biophysical Research Communications

Satoshi Inouye, Hironobu Hojo
To validate the use of recombinant aequorin (reAequorin) as a light emission standard, the protein concentrations of highly purified reAequorin were determined by amino acid composition analysis, and the presence of active reAequorin was confirmed by the ratio of absorbance peak at 460 nm to that at 280 nm. The high correlation of the luminescence intensity with the protein concentration showed that reAequorin could be used for a light emission standard to study the luminescence properties of luciferases and to evaluate the detection sensitivity of luminometers...
November 10, 2018: Biochemical and Biophysical Research Communications
Hongqiong Fan, Yuying Li, Chunshui Liu, Yingmin Liu, Jing Bai, Wei Li
Circular RNA (CirRNA) is a type of noncoding RNA that has been shown to play a unique role in tumor development and other fields in recent years. In this study, we aimed to explore the biological role of hsa_circ_100290 in acute myeloid leukemia (AML). First, we found that the expression of hsa_circ_100290 was increased in human AML samples and cell lines. Down-regulation of hsa_circ_100290 significantly suppressed cell proliferation of AML cells. Silencing hsa_circ_100290 also dramatically induced cell cycle arrest and apoptosis...
November 10, 2018: Biochemical and Biophysical Research Communications
Daliang Kong, Chang Li, Qi Yang, Bo Wei, Le Wang, Chuangang Peng
Long noncoding RNAs (lncRNAs) are recognized as a class of critical regulators in various tumors. Recently, lncRNA LSINCT5 has been reported to promote the progression of bladder cancer, ovarian cancer, gastric cancer, and breast cancer. However, the biological function of LSINCT5 remains elusive in osteosarcoma (OS). In our study, we found that LSINCT5 was significantly upregulated in OS tissues than in adjacent normal tissues. Additionally, the expression of LSINCT5 was inversely associated with the prognosis of patients with OS...
November 10, 2018: Biochemical and Biophysical Research Communications
Ailong Zhang, Tong Gao, Shenlan Wu, Zhenhui You, Junjie Zhen, Fun Wan
AIM: To determine the feasibility of using colloidal gold immunochromatography for rapid identification of parathyroid glands during thyroidectomy. MATERIAL AND METHODS: 127 patients undergoing thyroidectomy were randomly divided into PTH-ICGT group (64 cases) and conventional naked eye group (63 cases). The rate of identification of parathyroid glands and the incidence of hypoparathyroidism were compared between the two groups. RESULTS: PTH-CGI assay results showed that PTH concentration in the parathyroid tissue was (955...
November 9, 2018: Biochemical and Biophysical Research Communications
Shinya Endo, Nao Nishimura, Yawara Kawano, Niina Ueno, Shikiko Ueno, Hiro Tatetsu, Yoshihiro Komohara, Motohiro Takeya, Hiroyuki Hata, Hiroaki Mitsuya, Matsuoka Masao, Yutaka Okuno
The sialic glycoprotein, MUC1, is known to be involved in the pathogenesis of various types of cancers. KL-6 is one of the surface antigens of MUC1 and also a marker of interstitial pneumonitis. A fraction of patients with myeloma (3.9%) have elevated serum KL-6 levels without any evidence of interstitial pneumonitis and their myeloma cells have high MUC1 expression. We established a myeloma cell line designated EMM1 from a patient with multiple myeloma accompanied with elevated serum KL-6. EMM1 cells expressed high levels of MUC1 compared with other myeloma cell lines...
November 9, 2018: Biochemical and Biophysical Research Communications
Jiangbo Wan, Fang Huang, Weiwei Hu, Siguo Hao
To explore the role of type 1 regulatory T (Tr1) cells in allogeneic bone marrow transplantation (allo-BMT), we generated Tr1 cells from naïve CD4+ T cells in donor mice that were subjected to stimulation of recipient interleukin (IL)-10 gene-modified dendritic cells (DCs). In animal models, the severity of graft-versus-host disease (GVHD) in the group co-infused with DC-induced Tr1 cells was markedly milder than that in the groups without infusion of Tr1 cells. Moreover, the co-infusion Tr1 cells significantly prolonged the leukemia-free survival in tumor-bearing mice...
November 9, 2018: Biochemical and Biophysical Research Communications
Wenbo Li, Jin Wang
No abstract text is available yet for this article.
November 9, 2018: Biochemical and Biophysical Research Communications
Xinxi Li, Ye Tian, Yang Hu, Zhenwei Yang, Lei Zhang, Jun Luo
Circular (circ)RNAs have been implicated in cancer development. However, few studies have examined the role of circRNAs in papillary thyroid cancer (PTC). In this study we found that circNUP214 was upregulated in clinical PTC specimens relative to adjacent normal tissue. In vitro analyses showed that circNUP214 knockdown suppressed PTC cell proliferation, invasion, migration, and tumorigenesis. A luciferase reporter assay confirmed that circNUP214 binds to miR-145, which directly targets zinc finger E-box binding homeobox (ZEB)2...
November 8, 2018: Biochemical and Biophysical Research Communications
Md Motiar Rahman, Shigeyoshi Matsumura, Yoshiya Ikawa
In the RNA world, enrichment of self-replicating RNAs would have been beneficial to their survival, amplification, and evolution. Self-assembly of RNAs may be a strategy by which they enrich themselves. We examined the effects of molecular crowding on the activity of a bimolecular group I ribozyme and its derivative that self-assembles to form ribozyme oligomers. In a comparative activity assay using PEG as a molecular crowder, PEG rescued mutations in the parent bimolecular ribozyme more effectively than those in the oligomeric form...
November 8, 2018: Biochemical and Biophysical Research Communications
Guoping Ou, Qiaolun Chen, Wanli Liu, Xiaohui Li, Shulin Chen, Xingping Wu, Hao Chen
BACKGROUND: It was found that calcitonin in vitro can degrade rapidly whether in refrigeration or at room temperature, which increases the difficulty of accurate detecting of calcitonin greatly. This study was designed to solve this problem. METHODS: Serum samples with increased calcitonin from patients with medullary thyroid carcinoma were collected and divided into refrigeration and room temperature group. Analyze the degradation rule of calcitonin in the two groups according to the time of blood draw (T0), the time of sample detection (Tx), and the concentration (y) measured at Tx...
November 8, 2018: Biochemical and Biophysical Research Communications
L K Shewell, J J Wang, J C Paton, A W Paton, C J Day, M P Jennings
N-glycolylneuraminic acid (Neu5Gc)-containing glycans are a prominent form of aberrant glycosylation found in human tumor cells and have been proposed as cancer biomarkers. The B subunit of the subtilase cytotoxin (SubB) produced by Shiga toxigenic Escherichia coli recognises Neu5Gc containing glycans. We have previously engineered this lectin, SubB2M, for greater specificity and enhanced recognition of Neu5Gc-containing glycans. Here we further explore the utility of SubB2M to detect Neu5Gc tumor biomarkers in sera from patients with ovarian cancer...
November 8, 2018: Biochemical and Biophysical Research Communications
Yaming Jiu
Both the cytoskeletal intermediate filaments (IFs) and cytoplasmic caveolae contribute to active processes such as cell migration, morphogenesis and vesicular trafficking, but the interplay between these two systems has remained elusive. Here, we find that vimentin and nestin IFs interact with caveolae central component caveolin-1 (CAV-1) and importantly, restrain the intracellular trafficking of CAV-1 positive vesicles by serving as a physical barrier. Consequently, CAV-1 vesicles show less density and mobility in vimentin IFs enriched region, which is a substrate stiffness independent process...
November 8, 2018: Biochemical and Biophysical Research Communications
Toshihiro Kuremoto, Haruka Kogiso, Makoto Yasuda, Taka-Aki Inui, Kentaro Murakami, Shigeru Hirano, Yukiko Ikeuchi, Shigekuni Hosogi, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
Ciliary beating frequency (CBF) was investigated in ciliated nasal epithelial cells (cMNECs) isolated from mice using video microscopy equipped with a high-speed camera. In cMNECs, a spontaneous CBF oscillation was observed. The CBF oscillation was abolished by BAPTA-AM but not by Ca2+ -free solution. The addition of thapsigargin, which depletes Ca2+ from internal stores, also abolished CBF oscillation. Moreover, the intracellular Ca2+ concentration [Ca2+ ]i , spontaneously oscillated even with the Ca2+ -free solution...
November 8, 2018: Biochemical and Biophysical Research Communications
Chanbora Chea, Mutsumi Miyauchi, Toshihiro Inubushi, Kana Okamoto, Sivmeng Haing, Phuong Thao Nguyen, Hiromichi Imanaka, Takashi Takata
Epithelial-to-mesenchymal transition (EMT) is a biological process of invasion and metastasis in cancers, including in oral squamous cell carcinoma (OSCC). However, an effective anticancer drug that directly targets EMT has not yet been discovered. Therefore, we aimed to investigate the repressive effects of bovine lactoferrin (bLF) on EMT to achieve mesenchymal-to-epithelial transition (MET) in OSCC. OSCC cell lines, HOC313 (EMT-induced) and SCCVII (without EMT induction), were treated with bLF. The effects of bLF on EMT in OSCC were identified histologically by haematoxylin and eosin staining and observed morphologically and immunohistochemically using an anti-E-cadherin antibody...
November 8, 2018: Biochemical and Biophysical Research Communications
Lei Yang, Dan Wang, Xue-Ting Wang, Ya-Peng Lu, Li Zhu
BACKGROUND INFORMATION: Divalent metal transporter 1 (DMT1) and transferrin receptor (TfR1) are vital proteins for cellular iron uptake. These proteins have hypoxia-responsive elements (HREs) in their 5'-regulatory region, and they are regulated by hypoxia-inducible factor 1α (HIF-1α) transcriptionally under hypoxic condition. Besides, iron regulatory protein 1 (IRP1) regulates DMT1 and TfR1 by binding to iron-responsive elements (IREs) present in their mRNAs to control cellular iron homeostasis...
November 8, 2018: Biochemical and Biophysical Research Communications
Anusha Jahagirdar, Dandamudi Usharani, Malathi Srinivasan, Ram Rajasekharan
Brown adipose tissue (BAT) is the site of non-shivering thermogenesis in mammals, wherein energy is dissipated as heat. We observed that aqueous extract of black sesame seed triggers an increase in the expression of Uncoupling Protein 1 (UCP1) in brown adipocytes from mice. The active component from the extract was purified and identified to be sesaminol diglucoside (SDG). SDG treatment decreased mass of white fat pads and serum glucose levels and increased UCP1 levels in BAT thereby protecting mice against high fat induced weight gain...
November 8, 2018: Biochemical and Biophysical Research Communications
Wonjun Hong, Junghyun Park, Wonjin Yun, Phil Jun Kang, Daryeon Son, Jihoon Jang, In Yong Kim, Seungkwon You
Control of adipogenesis in mesenchymal stem cells (MSCs) offers enormous potential for management of obesity- and aging-related diseases. Celastrol, the traditional Chinese medicine extracted from Tripterygium wilfordi, exhibits anti-obesity effects in in vitro and in vivo murine models. This study describes how celastrol affects multilineage differentiation potential of human adipose-derived stem cells (hADSCs). We performed in vitro adipogenic differentiation of hADSCs and investigated how celastrol-induced lipid accumulation and expression of adipocyte differentiation markers varied with dose, duration, and donor age...
November 7, 2018: Biochemical and Biophysical Research Communications
Chan Bae Park, Vit-Na Choi, Jae-Bum Jun, Ji-Hae Kim, Youngsoo Lee, Jinhyuk Lee, GyuTae Lim, Jeonghyun Kim, Seon-Yong Jeong, Shin-Young Yim
Mitochondrial dysfunction and subsequent enhanced oxidative stress is implicated in the pathogenesis of autism spectrum disorder (ASD). Mitochondrial transcription factor B2 (TFB2M) is an essential protein in mitochondrial gene expression. No reports have described TFB2M mutations and variations involved in any human diseases. We identified a rare homozygous c.790C>T (His264Tyr) variation in TFB2M gene in two Korean siblings with ASD by whole-exome sequencing. The roles of the TFB2M variation in the pathogenesis of ASD were investigated...
November 7, 2018: Biochemical and Biophysical Research Communications
Huizi Keiko Li, Sumitaka Hasegawa, Nakako Izumi Nakajima, Yukie Morokoshi, Katsuyuki Minegishi, Kotaro Nagatsu
Major histocompatibility complex class I chain-related protein A and B (MICA/B) are ligands of the immune receptor, natural-killer group 2 member D. MICA/B expression is often found in several types of cancer but is restricted in normal tissues. Here, we show that an α-particle emitting astatine-211 (211 At)-labeled antibody targeting MICA/B (211 At-anti MICA/B Ab) efficiently ablates cancer cells in vitro and in vivo. We generated 211 At-anti MICA/B Ab, an anti-MICA/B antibody conjugated with a highly cytotoxic α-particle emitting radionuclide 211 At...
November 5, 2018: Biochemical and Biophysical Research Communications
Reezal Ishak, Maurice B Hallett
It has been proposed that Ca2+ activation of calpain-1 is an important trigger for rapid cell spreading by neutrophils. In this paper, we have investigated this by assessing the ex vivo functioning of neutrophils from calpain-1 null mice, Calpain-1 null neutrophils failed to migrate through TNF-activated endothelial monolayers. The failure to transmigrate through endothelial monolayers was therefore unlikely to be due to a failure of chemotaxis as chemotaxis by adherent calpain-1 null neutrophils towards fMLP was unpaired...
November 5, 2018: Biochemical and Biophysical Research Communications
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