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Archives of Biochemistry and Biophysics

Johannes von Lintig, Manfred Eggersdorfer, Adrian Wyss
No abstract text is available yet for this article.
September 15, 2018: Archives of Biochemistry and Biophysics
Meichun Zhang, Jing Wu, Weinong Zhong, Ziwen Zhao, Zhaohui Liu
Recently, several long non-coding RNAs (lncRNAs) have been revealed to play crucial roles in tumorigenesis and progression of many cancers. Nevertheless, more than 50,000 lncRNAs were identified in human cells and the roles of majority of these lncRNAs in non-small cell lung cancer (NSCLC) are unknown. In this study, using public NSCLC microarray data we identified a novel lncRNA BRE antisense RNA 1 (BRE-AS1). BRE-AS1 is significantly down-regulated in NSCLC tissues and cell lines. Gain-of-function and loss-of-function assays showed that BRE-AS1 reduces NSCLC cell viability, represses NSCLC cell proliferation, and induces NSCLC cell apoptosis in vitro, and represses NSCLC tumor growth in vivo...
September 15, 2018: Archives of Biochemistry and Biophysics
Gavin Morris, Naadira Pahad, Heini W Dirr, Sylvia Fanucchi
FOXP2 is a transcriptional repressor involved in development of the human brain and is the first gene product to be linked to the evolution of human speech. FOXP2 belongs to the FOX superfamily of proteins that share a common winged helix DNA binding domain - the forkhead domain. A divalent cation (Mg2+ or Ca2+ ) has been identified bound to a group of highly conserved residues in a number of FOX forkhead domain crystal structures. This work aims to investigate the role of the conserved divalent cation binding site by studying both the structure and DNA-binding function of the FOXP2 forkhead domain when in the presence and absence of either cation (Mg2+ or Ca2+ )...
September 15, 2018: Archives of Biochemistry and Biophysics
Xue Chen, Emmanuel Owoseni, Julia Salamat, Arthur I Cederbaum, Yongke Lu
Tobacco and alcohol are often co-abused. Nicotine can enhance alcoholic fatty liver, and CYP2A6 (CYP2A5 in mice), a major metabolism enzyme for nicotine, can be induced by alcohol. CYP2A5 knockout (cyp2a5-/- ) mice and their littermates (cyp2a5+/+ ) were used to test whether CYP2A5 has an effect on nicotine-enhanced alcoholic fatty liver. The results showed that alcoholic fatty liver was enhanced by nicotine in cyp2a5+/+ mice but not in the cyp2a5-/- mice. Combination of ethanol and nicotine increased serum triglyceride in cyp2a5+/+ mice but not in the cyp2a5-/- mice...
September 14, 2018: Archives of Biochemistry and Biophysics
Weijie Wen, Jianwen Chen, Liugang Ding, Xia Luo, Xueping Zheng, Qi Dai, Qianqian Gu, Cui Liu, Ming Liang, Xiaolei Guo, Peiqing Liu, Min Li
BACKGROUND: Astragalus membranaceus is a fundamental herb in Traditional Chinese Medicine and has attracted significant attention due to its anti-inflammatory, and longevity effects. However, its anti-photoaging property remains to be defined. Autophagy plays important roles in regulating cell homeostasis and aging processes. Whether regulation of autophagy could be an efficient way for anti-photoaging is still unclear. OBJECTIVE: To investigate the effects and the possible mechanism of astragaloside on anti-photoaging in UVB-induced photoaging cell model...
September 14, 2018: Archives of Biochemistry and Biophysics
Mayron Alves de Vasconcelos, Samara Sena da Penha, Vinícius Rodrigues Castro E Silva, Talita Abrante Leite, Elnatan Bezerra de Souza, Bartolomeu Warlene Silva Souza, Edson Holanda Teixeira, André Luis Coelho da Silva
Plant ribosome-inactivating proteins (RIPs) are a family of toxins that inhibit protein synthesis. In this study, we have isolated a novel type 2 ribosome-inactivating protein (RIP) present in seeds of the Abrus fruticulosus, named of fruticulosin. Fruticulosin, shows characteristics common to other type 2 RIPs, as specificity by galactosides (d-galactose, N-acetyl-d-galactosamine, and d-lactose), mass of approximately 60 kDa and presence of the of disulfide bonds. The N-terminal amino acid sequence (26 residues) of A-chain fruticulosin, determined by Edman degradation, revealed high similarity of the A-chain with those of other type 2 RIPs...
September 14, 2018: Archives of Biochemistry and Biophysics
Antara A Banerjee, Smita D Mahale
No abstract text is available yet for this article.
September 14, 2018: Archives of Biochemistry and Biophysics
Chinmayi R Kaundinya, Handanahal S Savithri, K Krishnamurthy Rao, Petety V Balaji
Bacillus subtilis 168 EpsC is annotated as "Probable polysaccharide biosynthesis protein" in the SwissProt database. epsC is part of the eps operon, thought to be involved in the biosynthesis of exopolymeric substances (EPS). The present study was undertaken to determine the molecular function of EpsC. Sequence analysis of EpsC suggested the presence of a transmembrane domain. Two N-terminal deletion mutants in which residues 1-89 (EpsC89 ) and 1-115 (EpsC115 ) are deleted were cloned and overexpressed...
September 14, 2018: Archives of Biochemistry and Biophysics
Jia-Heng Li, Zhan Zhang, Ming-Ze Du, Yi-Chun Guan, Jian-Ning Yao, Hai-Yang Yu, Bi-Jun Wang, Xing-Ling Wang, She-Ling Wu, Zhen Li
microRNA (miR)-141-3p has context-dependent effects on tumor progression. In this study, we attempted to explore the expression and function of miR-141-3p in cervical cancer. We found that miR-141-3p expression was significantly increased in cervical cancer specimens relative to normal cervical tissues. Moreover, miR-141-3p levels were associated with tumor size and lymph node metastasis status. Ectopic expression of miR-141-3p significantly increased cervical cancer cell proliferation, colony formation, invasion, and epithelial to mesenchymal transition, whereas depletion of miR-141-3p suppressed cervical cancer cell proliferation and invasion...
September 14, 2018: Archives of Biochemistry and Biophysics
Karim S Echtay, Martin Bienengraeber, Peter Mayinger, Simone Heimpel, Edith Winkler, Doerthe Druhmann, Karina Frischmuth, Frits Kamp, Shu-Gui Huang
The uncoupling protein (UCP1) is a proton (H+ ) transporter in the mitochondrial inner membrane. By dissipating the electrochemical H+ gradient, UCP1 uncouples respiration from ATP synthesis, which drives an increase in substrate oxidation via the TCA cycle flux that generates more heat. The mitochondrial uncoupling-mediated non-shivering thermogenesis in brown adipose tissue is vital primarily to mammals, such as rodents and new-born humans, but more recently additional functions in adult humans have been described...
September 11, 2018: Archives of Biochemistry and Biophysics
Kannikar Wongdee, Mayuree Rodrat, Chutiya Keadsai, Walailak Jantarajit, Jarinthorn Teerapornpuntakit, Jirawan Thongbunchoo, Narattaphol Charoenphandhu
Long-term high-calcium intake and intestinal calcium hyperabsorption are hazardous to the body. It is hypothesized that enterocytes possess mechanisms for preventing superfluous calcium absorption, including secretion of negative regulators of calcium absorption and utilization of calcium-sensing receptor (CaSR) to detect luminal calcium. Herein, Caco-2 monolayers were treated with high doses of 1,25(OH)2 D3 to induce calcium hyperabsorption or directly exposed to high apical calcium. The expression of counterregulatory factor of calcium absorption, fibroblast growth factor (FGF)-23, was also investigated in the intestine of lactating rats, which physiologically exhibit calcium hyperabsorption...
September 11, 2018: Archives of Biochemistry and Biophysics
Bai-Sui Feng, Yong-Jin Wu, Xian-Hai Zeng, Dian Yu, Zhi-Qiang Liu, Cai-Jie Zhou, Zhi-Gang Liu, Peng-Yuan Zheng, Ping-Chang Yang
The immune dysregulation plays an important role in the pathogenesis of ulcerative colitis (UC). Bcl2 like protein-12 (Bcl2L12) and mast cells are involved in immune dysregulation of UC. This study aims to elucidate the role of Bcl2L12 in the contribution to the pathogenesis of T helper (Th)2-biased inflammation in UC patients. The results showed that Bcl2L12 was expressed by peripheral CD4+ T cells that was associated with Th2 polarization in UC patients. Bcl2L12 mediated the protease-activated receptor-2 (PAR2)-induced IL-4 expression in CD4+ cells...
September 11, 2018: Archives of Biochemistry and Biophysics
Xinhang Yang, Brian Bennett, Richard C Holz
The functional roles of the (His)17 region and an insert region in the eukaryotic nitrile hydratase (NHase, EC from Monosiga brevicollis (MbNHase), were examined. Two deletion mutants, MbNHaseΔ238-257 and MbNHaseΔ219-272 , were prepared in which the (His)17 sequence and the entire insert region were removed. Each of these MbNHase enzymes provided an α2 β2 heterotetramer, identical to that observed for prokaryotic NHases and contains their full complement of cobalt ions. Deletion of the (His)17 motif provides an MbNHase enzyme that is ∼55% as active as the WT enzyme when expressed in the absence of the Co-type activator (ε) protein from Pseudonocardia thermophila JCM 3095 (PtNHaseact ) but ∼28% more active when expressed in the presence of PtNHaseact ...
September 8, 2018: Archives of Biochemistry and Biophysics
Parismita Kalita, Harish Shukla, Kundlik Gadhave, Rajanish Giri, Timir Tripathi
Thioredoxin glutathione reductase (TGRsec) is a multi-domain, flavoprotein that plays a principal role in redox homeostasis maintenance. We have previously demonstrated the role of selenocysteine in maintaining TGRsec structure-function, but the role of the glutaredoxin (Grx) domain and FAD is still unclear. In the present study, the urea-induced unfolding of recombinant Fasciola gigantica TGRsec (FgTGRsec) and its N-terminal truncated variant (ΔNTD-FgTGRsec) were examined to understand the role of the Grx domain and FAD in the stabilization of FgTGRsec and ΔNTD-FgTGRsec...
September 8, 2018: Archives of Biochemistry and Biophysics
Tatsuki Matsuzaki, Ayame Kano, Tetsuro Kamiya, Hirokazu Hara, Tetsuo Adachi
Non-thermal plasma (NTP) is applicable to living cells and has emerged as a novel technology for cancer therapy. Plasma-activated medium (PAM), which is prepared by the irradiation of culture medium with NTP, induces cell death in cancer cells. However, difficulties are associated with applying PAM to the clinical phase because culture media cannot be used for medical treatments. The objectives of the present study were to demonstrate the inhibitory effects of plasma-activated lactated Ringer's solution (PAL) on the viability of the A549 cancer cell line and elucidate the underlying mechanisms...
August 28, 2018: Archives of Biochemistry and Biophysics
Zi Wang, Yao Nie, Kunxiao Zhang, Henghao Xu, Theresa A Ramelot, Michael A Kennedy, Maili Liu, Jiang Zhu, Yunhuang Yang
SH2 domain-containing inositol 5-phosphatase 2 (SHIP2) binds with the Y1356-phosphorylated hepatocyte growth factor (HGF) receptor, c-MET, through its SH2 domain, which is essential for the role of SHIP2 in HGF-induced cell scattering and cell spreading. Previously, the experimental structure of the SH2 domain from SHIP2 (SHIP2-SH2) had not been reported, and its interaction with the Y1356-phosphorylated c-MET had not been investigated from a structural point of view. In this study, the solution structure of SHIP2-SH2 was determined by NMR spectroscopy, where it was found to adopt a typical SH2-domain fold that contains a positively-charged pocket for binding to phosphotyrosine (pY)...
August 27, 2018: Archives of Biochemistry and Biophysics
Priyanka Dubey, Sourabh Ghosh, Sauradipta Banerjee
This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause.
August 23, 2018: Archives of Biochemistry and Biophysics
Günter A Müller
Starting with the first description of the anchorage of a subset of cell surface proteins in eukaryotic cells from yeast to mammals with the aid of a glycosylphosphatidylinositol (GPI) moiety covalently attached to the carboxy-terminus of the protein, experimental evidence for the potential of GPI-anchored proteins (GPI-AP) of being released into the extracellular environment has been accumulating. GPI-AP are released as soluble monomers or multimers having lost their anchor or within hetero-/multimeric assemblies with their complete anchor remaining attached...
August 16, 2018: Archives of Biochemistry and Biophysics
Minghua Zou, Changhua Xu, Hua Li, Xianquan Zhang, Weidong Fan
Signal transducer and activator of transcription-3 (STAT3) protein is constitutively activated in ovarian cancer. The purpose of this study was to investigate the effects of 3,3'-diindolylmethane (DIM) on the regulation of STAT3 signaling and ovarian cancer cell viability, invasion, and sensitivity to chemotherapy. Ovarian cancer SKOV3 and A2780 cell lines were treated with various concentrations of DIM for different periods of time for assessment of cell viability as well as gene expression before and after knockdown of STAT3 expression using STAT3 shRNA...
July 21, 2018: Archives of Biochemistry and Biophysics
Clare Hawkins, Christian Obinger
No abstract text is available yet for this article.
October 1, 2018: Archives of Biochemistry and Biophysics
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