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Archives of Biochemistry and Biophysics

Seyede Elnaz Banijamali, Mehriar Amininasab, Mitra Maryam Elmi
A new member of kunitz-type protein family, PPTI (PseudocerastesPersicusTrypsin Inhibitor), was isolated from the venom of Persian false horned viper Pseudocerastes persicus and characterized. Mass spectrometry and amino acid sequencing revealed that PPTI is a 68 amino acid protein with molecular weight of about 7.6 kDa. The first amino acid residue of PPTI is N-terminally blocked via a post translational modification to pyroglutamyl. Sequence comparison against UniProtKB shows a high sequence similarity of PPTI with kunitz-type proteins, especially serine protease inhibitors and dendrotoxins (DTXs)...
November 16, 2018: Archives of Biochemistry and Biophysics
Hayden Hyatt, Rafael Deminice, Toshinori Yoshihara, Scott K Powers
Prolonged skeletal muscle inactivity (e.g. limb immobilization, bed rest, mechanical ventilation, spinal cord injury, etc.) results in muscle atrophy that manifests into a decreased quality of life and in select patient populations, a higher risk of morbidity and mortality. Thus, understanding the processes that contribute to muscle atrophy during prolonged periods of muscle disuse is an important area of research. In this regard, mitochondrial dysfunction has been directly linked to the muscle wasting that occurs during extended periods of skeletal muscle inactivity...
November 16, 2018: Archives of Biochemistry and Biophysics
Matthew R Battistini, Brian G O'Flynn, Christopher Shoji, Gabriela Suarez, Lamar C Galloway, David J Merkler
The arylalkylamine N-acyltransferases (AANATs) are enzymes that catalyze the acyl-CoA-dependent formation of N-acylarylalkylamides: acyl-CoA + arylalkylamine → N-acylarylalkylamides + CoA-SH. Herein, we describe our study of a previously uncharacterized AANAT from Bombyx mori: Bm-iAANAT3. Bm-iAANAT3 catalyzes the direct formation of N-acylarylalkylamides and accepts a broad range of short-chain acyl-CoA thioesters and amines as substrates. Acyl-CoA thioesters possessing an acyl chain length >10 carbon atoms are not substrates for Bm-iAANAT3...
November 16, 2018: Archives of Biochemistry and Biophysics
Renata Smulik-Izydorczyk, Michał Rostkowski, Angelika Gerbich, Dominika Jarmoc, Jan Adamus, Agnieszka Leszczyńska, Radosław Michalski, Andrzej Marcinek, Karol Kramkowski, Adam Sikora
The recent interest in the clinical applications of Piloty's acid derivatives as HNO donors for the treatment of cardiovascular system dysfunction has led us to the examination of factors controlling HNO release from selected ortho-substituted N-hydroxysulfonamides. Here we present the kinetic and quantum mechanical studies on the mechanism of HNO release from selected ortho-substituted N-hydroxysulfonamides and in vivo examination of the antiaggregatory properties of N-hydroxy-(2-bromobenzene)sulfonamide complex with sodium salt of β-cyclodextrin sulfobutyl ethers-ethyl ethers as compared with Angeli's salt...
November 15, 2018: Archives of Biochemistry and Biophysics
Massimo Bertinaria, Simone Gastaldi, Elisabetta Marini, Marta Giorgis
The NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome is the best recognized and most widely implicated regulator of caspase-1 activation. It is a key regulator of innate immune response and is involved in many pathophysiological processes. Recent evidences for its inappropriate activation in autoinflammatory, autoimmune, as well as in neurodegenerative diseases attract a growing interest toward the development of small molecules NLRP3 inhibitors. Based on the knowledge of biochemical and structural aspects of NLRP3 activation, one successful strategy in the identification of NLRP3 inhibitors relies on the development of covalent irreversible inhibitors...
November 15, 2018: Archives of Biochemistry and Biophysics
Paul M L Janssen
Myocardial relaxation is critical for the heart to allow for adequate filling of the ventricles prior to the next contraction. In human heart failure, impairment of myocardial relaxation is a major problem, and impacts most patients suffering from end-stage failure. Furthering our understanding of myocardial relaxation is critical in developing future treatment strategies. This review highlights processes involved in myocardial relaxation, as well as governing processes that modulate myocardial relaxation, with a focus on impairment of myocardium-level relaxation in human end-stage heart failure...
November 14, 2018: Archives of Biochemistry and Biophysics
Laudimir Leonardo Walbert Veloso-Silva, Paulo Roberto Dores-Silva, Dayane Eliara Bertolino Reis, Louis Fellipe Moreno Oliveira, Sílvia Helena Libardi, Júlio César Borges
First described in yeast in 1932 by Christian & Warburg, the Old Yellow Enzyme (OYE) (EC has aroused the interest of the scientific community regarding its high ability to catalyze stereoselective reactions of α/β-unsaturated carbonyl compounds with important industrial applications. In addition, the OYE family of proteins has been found in different organisms, such as plants, bacteria and protozoa, but not in mammals, which makes it an excellent candidate for a functional and molecular study aimed at more effective therapies with fewer undesirable side effects...
November 14, 2018: Archives of Biochemistry and Biophysics
Maria Gallo, Sira Defaus, David Andreu
In 1988, two unrelated papers reported the discovery of peptide vectors with innate cell translocation properties, setting the ground for a new area of research that over the years has grown into considerable therapeutic potential. The vectors, named cell-penetrating peptides (CPPs), constitute a now large and diversified family, sharing the extraordinary ability to diffuse unaltered across cell membranes while ferrying diverse associated cargos. Such properties have made CPPs ideal tools for delivery of nucleic acids, proteins and other therapeutic/diagnostic molecules to cells and tissues via covalent conjugation or complexation...
November 14, 2018: Archives of Biochemistry and Biophysics
Karissa M Dieseldorff Jones, Yeojung Koh, Rebecca S Weller, Rajdeep S Turna, Ferhaan Ahmad, Sabine Huke, Björn C Knollmann, Jose Renato Pinto, Hyun Seok Hwang
Mutations in cardiac troponin T (TnT) associated with hypertrophic cardiomyopathy generally lead to an increase in the Ca2+ sensitivity of contraction and susceptibility to arrhythmias. In contrast, TnT mutations linked to dilated cardiomyopathy decrease the Ca2+ sensitivity of contraction. Here we tested the hypothesis that two TnT disease mutations with opposite effects on myofilament Ca2+ sensitivity can attenuate each other's phenotype. We crossed transgenic mice expressing the HCM TnT-I79N mutation (I79N) with a DCM knock-in mouse model carrying the heterozygous TnT-R141W mutation (HET)...
November 13, 2018: Archives of Biochemistry and Biophysics
Matthew Triolo, David A Hood
Skeletal muscle mitochondria are essential in providing the energy required for locomotion. In response to contractile activity, the production of mitochondria is upregulated to meet the energy demands placed upon muscle cells. In a coordinated fashion, exercise also promotes the breakdown of dysfunctional mitochondria via mitophagy. Mitophagy is characterized by the selection of poorly functioning organelles, engulfment in an autophagosome and transport to lysosomes for degradation. In addition to the activation of mitophagy, exercise also elevates lysosome biogenesis...
November 12, 2018: Archives of Biochemistry and Biophysics
Tahir Ali, Iram Mushtaq, Sonia Maryam, Anam Farhan, Kiran Saba, Muhammad Ishtiaq Jan, Aneesa Sultan, Mariam Anees, Burcu Duygu, Sadia Hamera, Sobia Tabassum, Qamar Javed, Paula A da Costa Martins, Iram Murtaza
Early and specific diagnosis of oxidative stress linked diseases as cardiac heart diseases remains a major dilemma for researchers and clinicians. MicroRNAs may serve as a better tool for specific early diagnostics and propose their utilization in future molecular medicines. We aimed to measure the microRNAs expressions in oxidative stress linked cardiac hypertrophic condition induced through stimulants as Endothelin and Isoproterenol. Cardiac hypertrophic animal models were confirmed by BNP, GATA4 expression, histological assays, and increased cell surface area...
November 12, 2018: Archives of Biochemistry and Biophysics
Huayi Huang, Oreste Salavaggione, Lee Rivera, Sarbajit Mukherjee, Rolf Brekken, Bud Tennant, Renuka Iyer, Araba Adjei
Vascular endothelial growth factor (VEGF) stimulates angiogenesis. Human hepatocellular carcinoma (HCC) is a VEGF-driven tumor often associated with chronic hepatitis B or C virus infection. The woodchuck is a well-characterized model of hepatitis B virus related HCC and a valuable tool for translational studies of novel VEGF targeted agents. We cloned the cDNA encoding woodchuck VEGF (wVEGF), transiently expressed it in COS cells and functionally characterized the recombinant protein. The open reading frame of wVEGF contained 645 nucleotides encoding a protein of 214 amino acids...
November 12, 2018: Archives of Biochemistry and Biophysics
Fernanda Dal'Maso Camera, Bruna Gianatassio Pozzi, Carla de Souza Paganini, Helen Rebelo Sorato, Fernanda Tavares, Bárbara da Costa Pereira, Giulia S Pedroso, Silvane Souza Roman, Paulo Cesar Lock Silveira, Renata Tiscoski Nesi, Ricardo Aurino Pinho
The present study sought to evaluate the effects of physical training on histological parameters and oxidative stress in the myocardium of mice chronically exposed to hand-rolled cornhusk cigarette (HRCC) smoke. Male Swiss mice (60 days old, 30-35 g) were either exposed to ambient air or passively exposed to the smoke of 12 cigarettes daily over 3 sessions (4 cigarettes per session) for 60 consecutive days with or without physical training for 8 weeks. Forty-eight hours after the last training session, the heart was surgically removed for histological analysis and measurement of oxidative stress parameters...
November 8, 2018: Archives of Biochemistry and Biophysics
Radhakrishnan Mahalakshmi
Non-covalent interactions between naturally occurring aromatic residues have been widely exploited as scaffold stabilizing agents in de novo designed peptides and in Nature - inspired structures. Our understanding of the factors driving aromatic interactions and their observed interaction geometries have advanced remarkably with improvements in conventional structural studies, availability of novel molecular methods and in silico studies, which have together provided atomistic information on aromatic interactions and interaction strengths...
November 3, 2018: Archives of Biochemistry and Biophysics
Rebekka Mauser, Albert Jeltsch
Various post-translational modifications (PTMs) have been identified on histone proteins, which occur at hundreds of different sites. Histone PTMs influence the chromatin structure and serve as binding sites for reading domains, which further mediate downstream effects. Histone PTM antibodies or recombinant proteins derived from reading domains are unique research reagents essentially required to study histone modifications. To validate their specificity, histone PTM peptide arrays are used, because they allow to investigate the binding of proteins to a large number of different peptides in one experiment...
November 2, 2018: Archives of Biochemistry and Biophysics
Mingzhi Zhu, Xiaochun Wang, Yuanting Gu, Fang Wang, Lin Li, Xinguang Qiu
Increasing studies have highlighted the critical role of lncRNAs in cancer pathogenesis and development. LncRNA maternally expressed gene 3 (MEG3) was reported to function as a tumor suppressor in breast cancer. However, the detailed molecular mechanism of MEG3 involved in breast cancer progression remains far from being addressed. Our findings showed that MEG3 was downregulated and miR-21 was upregulated in breast cancer patient tissues and cells. MEG3 overexpression suppressed cell proliferation and glycolysis, and induced apoptosis in breast cancer cells...
October 31, 2018: Archives of Biochemistry and Biophysics
María-José Dávila-Rodríguez, João Paulo Barolli, Katia Mara de Oliveira, Legna Colina-Vegas, Fábio da Silva Miranda, Eduardo Ernesto Castellano, Gustavo Von Poelhsitz, Alzir Azevedo Batista
This paper describes on the interaction studies of carbonyl heterobimetallic compounds of Ru(II)/Fe(II) containing polypyridyl ligands, with general formula ct-[RuCl(CO)(N-N)(dppf)]PF6 , N-N = 1,10-phenanthroline (phen) 5; dipyrido[3,2-f:2',3'-h]quinoxaline (dpq) 6; dipyrido[3,2-a:2',3'-c]phenazine (dppz) 7; dipyrido[3,2-f:2',3'-h]quinoxalino[2,3-b]quinoxaline (dpqQX) 8 and dppf = 1,1'-bis(diphenylphosphino) ferrocene], with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA). Also, it describes the cellular viability assays of these complexes in tumorigenic and non-tumorigenic cell lines...
October 31, 2018: Archives of Biochemistry and Biophysics
Zhuqing Zha, Anping Su, Shaochuan Huo
Estrogenic signals can regulate the progression of osteosarcoma (OS) via classic estrogen receptor α/β (ERα/β). G protein-coupled estrogen receptor (GPER) can mediate the non-genomic effects of estrogen and regulate the progression of various cancers. Our present study revealed that the expression of GPER in OS cells and tissues was lower than that in their corresponding controls. Activation of GPER via its specific agonist G-1 can decrease the proliferation, migration, and invasion of OS cells. By screening the expression of cytokines involved in the progression of OS, we found that activation of GPER can inhibit the expression of interleukin-6 (IL-6) and IL-8 in OS cells...
October 30, 2018: Archives of Biochemistry and Biophysics
Takuo Ogiso, Tatsuki Fukami, Kenji Mishiro, Keigo Konishi, Jeffrey P Jones, Miki Nakajima
Human aldehyde oxidase 1 (AOX1) catalyzes the oxidation of various drugs and endogenous compounds. Recently, we found that AOX1 catalyzed the reduction of drugs such as nitrazepam and dantrolene. In this study, we aimed to clarify the substrate selectivity of human AOX1 for the reduction of nitroaromatic drugs to obtain helpful information for drug development. We investigated whether 11 nitroaromatic drugs were reduced by AOX1 using recombinant AOX1 and human liver cytosol (HLC) in the presence of N1 -methylnicotinamide, an electron donor to AOX1...
October 25, 2018: Archives of Biochemistry and Biophysics
Eric Adua, Elham Memarian, Alyce Russell, Irena Trbojević-Akmačić, Ivan Gudelj, Julija Jurić, Peter Roberts, Gordan Lauc, Wei Wang
Aberrant protein glycosylation may reflect changes in cell metabolism of type II diabetes mellitus (T2DM) and offers fresh vistas for discovering potential biomarkers. However, the functional significance of T2DM N-glycan alterations is underexplored, since to date, N-glycan profiling studies have been performed in selected populations. Geographically and genetically isolated populations are needed for validation of specific biomarkers. This age-sex matched cross sectional study comprising 232 T2DM patients and 219 controls was conducted in Ghana, Western Africa...
October 24, 2018: Archives of Biochemistry and Biophysics
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