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Archives of Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/28941803/dj-1-activates-autophagy-in-the-repression-of-cardiac-hypertrophy
#1
Ruicong Xue, Jingzhou Jiang, Bin Dong, Weiping Tan, Yu Sun, Jingjing Zhao, Yili Chen, Yugang Dong, Chen Liu
Cardiac hypertrophy is the risk factor of heart failure when the heart is confronted with pressure overload or neurohumoral stimuli. Autophagy, a conserved degradative pathway, is one of the important mechanisms involved in the regulation of cardiac hypertrophy. DJ-1 is a traditional anti-oxidative protein and emerging evidence suggested that DJ-1 might modulate autophagy. However, the regulation of autophagy by DJ-1 in the process of cardiac hypertrophy remains unknown. In our study, we firstly discovered that the expression of DJ-1declined in the process of pressure overload cardiac hypertrophy, and its alteration was parallel with the impairment of autophagy...
September 20, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28941802/cftr-modulates-rps27-gene-expression-using-chloride-anion-as-signaling-effector
#2
Ángel G Valdivieso, Consuelo Mori, Mariángeles Clauzure, Macarena Massip-Copiz, Tomás A Santa-Coloma
In Cystic Fibrosis (CF), the impairment of the CFTR channel activity leads to a variety of alterations, including differential gene expression. However, the CFTR signaling mechanisms remain unclear. Recently, culturing IB3-1 CF cells under different intracellular Cl(-) concentrations ([Cl(-)]i), we observed several Cl(-)-dependent genes and further characterized one of them as RPS27. Thus, we hypothesized that Cl(-) might act as a signaling effector for CFTR signaling. Here, to test this idea, we study RPS27 expression in T84 cells modulating the CFTR activity by using CFTR inhibitors...
September 20, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28939102/reaction-rates-of-glutathione-and-ascorbate-with-alkyl-radicals-are-too-slow-for-protection-against-protein-peroxidation-in-vivo
#3
Thomas Nauser, Janusz M Gebicki
Reaction kinetics of amino acid and peptide alkyl radicals with GSH and ascorbate, the two most abundant endogenous antioxidants, were investigated by pulse radiolysis. Rate constants in the order of 10(6) M(-1)s(-1) were found. Alkyl radicals react at almost diffusion controlled rates and irreversibly with oxygen to form peroxyl radicals, and competition with this reaction is the benchmark for efficient repair in vivo. We consider repair of protein radicals and assume comparable rate constants for the reactions of GSH/ascorbate with peptide alkyl radicals and with alkyl radicals on a protein surface...
September 19, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28927883/retinol-saturase-modulates-lipid-metabolism-and-the-production-of-reactive-oxygen-species
#4
Xiao-Yan Pang, Suya Wang, Michael J Jurczak, Gerald I Shulman, Alexander R Moise
Retinol saturase (RetSat) catalyzes the saturation of double bonds of all-trans-retinol leading to the production of dihydroretinoid metabolites. Beside its role in retinoid metabolism, there is evidence that RetSat modulates the cellular response to oxidative stress and plays critical roles in adipogenesis and the accumulation of lipids. Here, we explore the relationship between RetSat, lipid metabolism and oxidative stress using in vitro and in vivo models with altered expression of RetSat. Our results reveal that RetSat is a potent modulator of the cellular response to oxidative stress and the generation of reactive oxygen species (ROS)...
September 18, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28919057/adp-dependent-phosphofructokinases-from-the-archaeal-order-methanosarcinales-display-redundant-glucokinase-activity
#5
Ricardo A Zamora, Felipe Gonzalez-Órdenes, Victor Castro-Fernández, Victoria Guixé
The genome of Methanosarcinales organisms presents both ADP-dependent glucokinase and phosphofructokinase genes. However, Methanococcoides burtonii has a truncate glucokinase gene with a large deletion at the C-terminal, where the catalytic GXGD motif is located. Characterization of its phosphofructokinase annotated protein shows that is a bifunctional enzyme able to supply the absence of the glucokinase activity. Moreover, kinetic analyses of the phosphofructokinase annotated enzyme from, Methanohalobium evestigatum demonstrated that this enzyme is also bifunctional...
September 14, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28917600/atp-alters-protein-folding-and-function-of-escherichia-coli-uridine-phosphorylase
#6
Yi-Kai Liu, Tzu-Hsuan Lin, Pei-Fen Liu
Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. However, the mechanism underlying the destabilization process and its influence on uridine phosphorylase function remain to be established. Here, we comprehensively investigated the effects of ATP on protein folding and function of Escherichia coli uridine phosphorylase...
September 13, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28893509/non-thermal-plasma-induces-mitochondria-mediated-apoptotic-signaling-pathway-via-ros-generation-in-hela-cells
#7
Wei Li, K N Yu, Jie Ma, Jie Shen, Cheng Cheng, Fangjian Zhou, Zhiming Cai, Wei Han
Non-thermal plasma (NTP) has been proposed as a novel therapeutic method for anticancer treatment. Although increasing evidence suggests that NTP selectively induces apoptosis in some types of tumor cells, the molecular mechanisms underlying this phenomenon remain unclear. In this study, we further investigated possible molecular mechanisms for NTP-induced apoptosis of HeLa cells. The results showed that NTP exposure significantly inhibited the growth and viability of HeLa cells. Morphological observation and flow cytometry analysis demonstrated that NTP exposure induced HeLa cell apoptosis...
September 9, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28893510/the-soluble-domains-of-gpi8-and-gaa1-two-subunits-of-glycosylphosphatidylinositol-transamidase-gpi-t-assemble-into-a-complex
#8
Dilani G Gamage, Yug Varma, Jennifer L Meitzler, Rachel Morissette, Travis J Ness, Tamara L Hendrickson
Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active site subunit of GPI-T, is part of a hetero-pentameric complex containing Gpi16, Gaa1, Gpi17, and Gab1. Gpi8, Gaa1, and Gpi16 co-purify as a heterotrimer from Saccharomyces cerevisiae, suggesting that they form the core of the GPI-T. Details about the assembly and organization of these subunits have been slow to emerge. We have previously shown that the soluble domain of S...
September 8, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28870454/introduction-to-flavoproteins-beyond-the-classical-paradigms
#9
EDITORIAL
Pablo Sobrado, Giovanni Gadda
No abstract text is available yet for this article.
September 8, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28888872/the-catalytic-activity-of-a-recombinant-single-chain-variable-fragment-nucleic-acid-hydrolysing-antibody-varies-with-fusion-tag-and-expression-host
#10
Joungmin Lee, Minjae Kim, Youngsil Seo, Yeonjin Lee, Hyunjoon Park, Sung June Byun, Myung-Hee Kwon
The antigen-binding properties of single chain Fv antibodies (scFvs) can vary depending on the position and type of fusion tag used, as well as the host cells used for expression. The issue is even more complicated with a catalytic scFv antibody that binds and hydrolyses a specific antigen. Herein, we investigated the antigen-binding and -hydrolysing activities of the catalytic anti-nucleic acid antibody 3D8 scFv expressed in Escherichia coli or HEK293f cells with or without additional amino acid residues at the N- and C-termini...
September 6, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28888871/interplay-of-mitochondria-apoptosis-regulatory-factors-and-micrornas-in-valvular-heart-disease
#11
Muhammad Ishtiaq Jan, Riaz Anwar Khan, Tahir Ali, Muhammad Bilal, Long Bo, Abdul Sajid, Abdul Malik, Naseeb Urehman, Nayyar Waseem, Javed Nawab, Murad Ali, Abdul Majeed, Hamid Ahmad, Sohail Aslam, Sadia Hamera, Aneesa Sultan, Mariam Anees, Qamar Javed, Iram Murtaza
Valvular heart disease (VHD) is an active process involving a wide range of pathological changes. The major complications of VHD are stenosis and regurgitation, which are macroscopic phenomena, induced in part through cellular changes. Altered expression of mitochondria associated genes causes membrane potential depolarization, leading to the increased levels of apoptosis observed in cardiac dysfunction. Objective of this study is to find molecular medicine candidates that can control expression of the key mitochondria apoptosis regulatory genes...
September 6, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28887129/disulfiram-is-a-slow-binding-partial-noncompetitive-inhibitor-of-20s-proteasome-activity
#12
Brian B Hasinoff, Daywin Patel
The alcohol abuse drug disulfiram has also been shown to exhibit potent cell growth inhibitory and anticancer activity. While a number of cellular and animal studies have suggested that disulfiram exhibits its anticancer activity through interaction with the proteasome, direct evidence for inhibition of proteasome activity is lacking. In this study we show that disulfiram potently inhibits the chymotrypsin-like activity of purified human 20S proteasome at low micromolar pharmacological concentrations. The enzyme progress curves displayed characteristics of a slow-binding reaction, similar to that observed for the FDA-approved proteasomal-targeted anticancer drugs bortezomib and carfilzomib...
September 5, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28882636/bisphenol-a-and-estrogen-induce-proliferation-of-human-thyroid-tumor-cells-via-an-estrogen-receptor-dependent-pathway
#13
YongHong Zhang, Feng Wei, Jing Zhang, Lanxiang Hao, Jie Jiang, Liansheng Dang, Dan Mei, ShanShan Fan, Yajin Yu, Ling Jiang
OBJECTIVE: To determine the relationship between papillary thyroid carcinoma and environmental exposure to bisphenol A (BPA) or 17-β estrogen (E2) by assessing the effects of these compounds on estrogen receptor expression and AKT/mTOR signaling. METHODS: The effects of low levels of BPA (1mM-10nM) and 17β-estradiol (E2, 0.1mM-1nM) on ER expression and cellular proliferation were determined in human thyroid papillary cancer BHP10-3 cells. Protein and mRNA levels of estrogen nuclear receptors (ERα/ERβ) and membrane receptors (GPR30) were determined by immunofluorescence assay, Western blotting, and RT-PCR, respectively, and proliferation was assessed by CCK-8 assay...
September 4, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28842127/mechanistic-insights-into-eggst1-a-mu-class-glutathione-s-transferase-from-the-cestode-parasite-echinococcus-granulosus
#14
Paula Arbildi, Lucía Turell, Verónica López, Beatriz Alvarez, Verónica Fernández
Glutathione transferases (GSTs) comprise a major detoxification system in helminth parasites, displaying both catalytic and non-catalytic activities. The kinetic mechanism of these enzymes is complex and depends on the isoenzyme which is being analyzed. Here, we characterized the kinetic mechanism of rEgGST1, a recombinant form of a cytosolic GST from Echinococcus granulosus (EgGST1), which is related to the Mu-class of mammalian enzymes, using the canonical substrates glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB)...
August 30, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28851624/effect-of-conservative-mutations-l94v-and-l94i-on-the-structure-and-stability-of-horse-cytochrome-c
#15
Sabab Hasan Khan, Asimul Islam, Md Imtaiyaz Hassan, Sujata Sharma, Tej Pal Singh, Faizan Ahmad
A sequence alignment of horse cytochrome c (cyt c) with all known cyts c shows that Leu at position 94 is conserved, except in 14 species which have either Val or Ile at this position. It is also known that Leu94 of the mammalian cyt c plays an important role in folding and stability. The important question here is as to what will happen in terms of folding and stability if Leu94 of the mammalian cyt c is substituted by Val or Ile. To answer this question, we introduced natural substitutes of Leu94 by Val and Ile in horse cyt c...
August 26, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28822840/role-of-adp-ribosylation-factor6-cytohesin1-phospholipased-signaling-axis-in-u46619-induced-activation-of-nadph-oxidase-in-pulmonary-artery-smooth-muscle-cell-membrane
#16
Sajal Chakraborti, Jaganmay Sarkar, Animesh Chowdhury, Tapati Chakraborti
Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cell membrane contains predominantly PLD2 along with PLD1 isoforms of PLD. Pretreatment with pharmacological and genetic inhibitors of PLD2, but not PLD1, attenuated U46619 stimulation of NADPH oxidase activity...
August 16, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28821425/flavin-dependent-thymidylate-synthase-n5-of-flavin-as-a-methylene-carrier
#17
REVIEW
Kalani Karunaratne, Nicholas Luedtke, Daniel M Quinn, Amnon Kohen
Thymidylate is synthesized de novo in all living organisms for replication of genomes. The chemical transformation is reductive methylation of deoxyuridylate at C5 to form deoxythymidylate. All eukaryotes including humans complete this well-understood transformation with thymidylate synthase utilizing (6R)-N(5)-N(10)-methylene-5,6,7,8-tetrahydrofolate as both a source of methylene and a reducing hydride. In 2002, flavin-dependent thymidylate synthase was discovered as a new pathway for de novo thymidylate synthesis...
August 15, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28810138/corrigendum-to-a-comprehensive-review-on-the-colourless-carotenoids-phytoene-and-phytofluene-arch-biochem-biophys-572-2015-188-200
#18
Antonio J Meléndez-Martínez, Paula Mapelli-Brahm, Ana Benítez-González, Carla M Stinco
No abstract text is available yet for this article.
August 12, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28803837/silencing-of-glut1-induces-chemoresistance-via-modulation-of-akt-gsk-3%C3%AE-%C3%AE-catenin-survivin-signaling-pathway-in-breast-cancer-cells
#19
Sunhwa Oh, KeeSoo Nam, Hyungjoo Kim, Incheol Shin
Cancer cells require increased aerobic glycolysis to support rapid cell proliferation. For their increased energy demands, cancer cells express glucose transporter (Glut) proteins at a high level. Glut1 is associated with basal-like breast cancer and is considered a potential therapeutic target. To investigate the possibility of Glut1 as a therapeutic target in breast cancer cells, we downregulated Glut1 in triple-negative breast cancer (TNBC) cell lines using a short hairpin system. We determined whether Glut1 silencing might enhance anti-proliferative effect of chemotherapeutic agents...
August 10, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28802828/photolyase-dynamics-and-electron-transfer-mechanisms-of-dna-repair
#20
REVIEW
Meng Zhang, Lijuan Wang, Dongping Zhong
Photolyase, a flavoenzyme containing flavin adenine dinucleotide (FAD) molecule as a catalytic cofactor, repairs UV-induced DNA damage of cyclobutane pyrimidine dimer (CPD) and pyrimidine-pyrimidone (6-4) photoproduct using blue light. The FAD cofactor, conserved in the whole protein superfamily of photolyase/cryptochromes, adopts a unique folded configuration at the active site that plays a critical functional role in DNA repair. Here, we review our comprehensive characterization of the dynamics of flavin cofactor and its repair photocycles by different classes of photolyases on the most fundamental level...
August 9, 2017: Archives of Biochemistry and Biophysics
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