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Archives of Biochemistry and Biophysics

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https://www.readbyqxmd.com/read/29154781/eucalyptus-globulus-extract-protects-against-uvb-induced-photoaging-by-enhancing-collagen-synthesis-via-regulation-of-tgf-%C3%AE-smad-signals-and-attenuation-of-ap-1
#1
Bom Park, Eunson Hwang, Seul A Seo, Jin-Gyeong Cho, Jung-Eun Yang, Tae-Hoo Yi
UV irradiation triggers the overproduction of matrix metalloproteinases and collagen degradation, which in turn causes increased pigmentation, dryness, and deep wrinkling of the skin. These chronic symptoms are collectively referred to as photoaging. Eucalyptus globulus is an evergreen tree that is widely used in cosmetics because of its antimicrobial activity. In this study, we investigated the protective effect of 50% ethanol extracts of Eucalyptus globulus on UV-induced photoaging in vitro and in vivo. Normal human dermal fibroblasts were treated with Eucalyptus globulus at concentrations ranging from 1 to 100 μg/mL after UVB or non-UVB irradiation...
November 14, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29138096/one-single-salt-bridge-explains-the-different-cytolytic-activities-shown-by-actinoporins-sticholysin-i-and-ii-from-the-venom-of-stichodactyla-helianthus
#2
Esperanza Rivera-de-Torre, Juan Palacios-Ortega, Sara García-Linares, José G Gavilanes, Álvaro Martínez-Del-Pozo
Sticholysins I and II (StnI and StnII), α-pore forming toxins from the sea anemone Stichodactyla helianthus, are water-soluble toxic proteins which upon interaction with lipid membranes of specific composition bind to the bilayer, extend and insert their N-terminal α-helix, and become oligomeric integral membrane structures. The result is a pore that leads to cell death by osmotic shock. StnI and StnII show 93% of sequence identity, but also different membrane pore-forming activities. The hydrophobicity profile along the first 18 residues revealed differences which were canceled by substituting StnI amino acids 2 and 9...
November 11, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29138095/kinetics-and-thermodynamics-of-the-thermal-inactivation-and-chaperone-assisted-folding-of-zebrafish-dihydrofolate-reductase
#3
Charu Thapliyal, Neha Jain, Naira Rashid, Pratima Chaudhuri
The maintenance of thermal stability is a major issue in protein engineering as many proteins tend to form inactive aggregates at higher temperatures. Zebrafish DHFR, an essential protein for the survival of cells, shows irreversible thermal unfolding transition. The protein exhibits complete unfolding and loss of activity at 50 °C as monitored by UV-Visible, fluorescence and far UV-CD spectroscopy. The heat induced inactivation of zDHFR follows first-order kinetics and Arrhenius law. The variation in the value of inactivation rate constant, k with increasing temperatures depicts faster inactivation at elevated temperatures...
November 11, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29080630/effect-of-quercetin-on-cell-protection-via-erythropoietin-and-cell-injury-of-hepg2-cells
#4
Kazuhiko Nishimura, Risa Matsumoto, Yuuki Yonezawa, Hiroshi Nakagawa
Quercetin is a flavonoid that has roles in both cytoprotection and cytotoxicity. The relation of queretin's cytoprotective and cytotoxic effects are unknown. Quercetin has been shown to induce expression of hypoxia-inducible factor, a protein that is known to regulate transcription of the erythropoietin (EPO) gene, and EPO is known to have a cytoprotective effect. This study used HepG2 cells to assess whether the cell-protective and/or cytotoxic roles of quercetin are mediated by promotion of EPO production...
November 8, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29126969/snhg16-mir-216-5p-zeb1-signal-pathway-contributes-to-the-tumorigenesis-of-cervical-cancer-cells
#5
Hong Zhu, Yan Zeng, Chen-Chen Zhou, Weiping Ye
Long non-coding RNAs (lncRNAs) have been confirmed as crucial regulators in tumorgenesis. Small nucleolar RNA host gene 16 (SNHG16) has been recently uncovered to be a potential oncogene in several types of cancers. However, its expression level and potential role in cervical cancer remain uncertain. In our research, we assessed the expression level of SNHG16 in clinical cervical cancer tissues and cells. We made use of functional assays to determine the biological effects of SNHG16 on cell proliferation and migration of cervical cancer...
November 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29126968/jnk-signaling-pathway-regulates-sorbitol-induced-tau-proteolysis-and-apoptosis-in-sh-sy5y-cells-by-targeting-caspase-3
#6
Marta Olivera Santa-Catalina, Montaña Caballero Bermejo, Ricardo Argent, Juan C Alonso, Francisco Centeno, María J Lorenzo
Growing evidence suggests that Diabetes Mellitus increases the risk of developing Alzheimer's disease. It is well known that hyperglycemia, a key feature of Diabetes Mellitus, may induce plasma osmolarity disturbances. Both hyperglycemia and hyperosmolarity promote the altered post-translational regulation of microtubule-associated protein Tau. Interestingly, abnormal hyperphosphorylation and cleavage of Tau have been proven to lead to the genesis of filamentous structures referred to as neurofibrillary tangles, the main pathological hallmark of Alzheimer's disease...
November 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29122589/naringin-prevents-the-inhibition-of-intestinal-ca-2-absorption-induced-by-a-fructose-rich-diet
#7
V Rodríguez, M Rivoira, S Guizzardi, N Tolosa de Talamoni
This study tries to elucidate the mechanisms by which fructose rich diets (FRD) inhibit the rat intestinal Ca(2+) absorption, and determine if any or all underlying alterations are prevented by naringin (NAR). Male rats were divided into: 1) controls, 2) treated with FRD, 3) treated with FRD and NAR. The intestinal Ca(2+) absorption and proteins of the transcellular and paracellular Ca(2+) pathways were measured. Oxidative/nitrosative stress and inflammation parameters were evaluated. FRD rats showed inhibition of the intestinal Ca(2+) absorption and decrease in the protein expression of molecules of both Ca(2+) pathways, which were blocked by NAR...
November 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29037962/mild-palmitate-treatment-increases-mitochondrial-mass-but-does-not-affect-ea-hy926-endothelial-cells-viability
#8
Dorota Dymkowska, Maria Kawalec, Tomasz Wyszomirski, Krzysztof Zabłocki
A dyslipidaemia-related increase of the concentration of long-chain fatty acids in the plasma is an important pathological factor substantially increasing risk of serious consequences in vascular endothelium. Inflammatory response, atherosclerosis and insulin resistance seem the most severe. Palmitate at excessive concentrations has been shown to have a harmful effect on endothelial cells impairing NO generation, stimulating reactive oxygen species (ROS) formation and affecting their viability. On the other hand we found that palmitate applied for 48 h at 100 μM concentration which is sufficient to induce inflammatory response, increase ROS generation and reduce insulin sensitivity of EA...
November 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29122590/physiological-serum-copper-concentrations-found-in-malignancies-cause-unfolding-induced-aggregation-of-human-serum-albumin-in-vitro
#9
Asim Rizvi, Mohd Furkan, Imrana Naseem
Malignancies are characterized by several drastic metabolic changes, one of which is a progressive rise in the levels of serum copper. This rise in serum copper is documented across all malignancies and across malignancies in several species. This study aims to explore in vitro the effect of increased copper levels on the structure of the blood protein human serum albumin. Exposure of human serum albumin to physiologically relevant copper concentrations for 21 days resulted in structural modifications in the protein which were evident by changes in the intrinsic florescence...
November 6, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29100984/sexual-dimorphism-in-oxidant-induced-adaptive-homeostasis-in-multiple-wild-type-d-melanogaster-strains
#10
Laura C D Pomatto, Sarah Wong, John Tower, Kelvin J A Davies
Sexual dimorphism includes the physical and reproductive differences between the sexes, including differences that are conserved across species, ranging from the common fruit fly, Drosophila melanogaster, to humans. Sex-dependent variations in adaptive homeostasis, and adaptive stress responses may offer insight into the underlying mechanisms for male and female survival differences and into differences in chronic disease incidence and severity in humans. Earlier work showed sex-specific differences in adaptive responses to oxidative stressors in hybrid laboratory strains of D...
October 31, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29100983/acetyl-coa-carboxylase-in-escherichia-coli-exhibits-a-pronounced-hysteresis-when-inhibited-by-palmitoyl-acyl-carrier-protein
#11
Alexandra Evans, Wendy Ribble, Erin Schexnaydre, Grover L Waldrop
Acetyl-CoA carboxylase (ACC) in bacteria is composed of three components: biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase. ACC catalyzes the first committed step in fatty acid synthesis: the carboxylation of acetyl-CoA to form malonyl-CoA via a two-step reaction. In the first half-reaction, biotin carboxylase catalyzes the ATP-dependent carboxylation of the vitamin biotin covalently linked to biotin carboxyl carrier protein. In the second half-reaction, the carboxyl group is transferred from biotin to acetyl-CoA by the enzyme carboxyltransferase...
October 31, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29100982/thermodynamics-of-cooperative-binding-of-fad-to-human-nqo1-implications-to-understanding-cofactor-dependent-function-and-stability-of-the-flavoproteome
#12
Rafael Clavería-Gimeno, Adrian Velazquez-Campoy, Angel Luis Pey
The stability of human flavoproteins strongly depends on flavin levels, although the structural and energetic basis of this relationship is poorly understood. Here, we report an in-depth analysis on the thermodynamics of FAD binding to one of the most representative examples of such relationship, NAD(P)H:quinone oxidoreductase 1 (NQO1). NQO1 is a dimeric enzyme that tightly binds FAD, which triggers large structural changes upon binding. A common cancer-associated polymorphism (P187S) severely compromises FAD binding...
October 31, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29097312/insights-on-the-conformational-dynamics-of-human-frataxin-through-modifications-of-loop-1
#13
Martín E Noguera, Martín Aran, Clara Smal, Diego S Vazquez, María Georgina Herrera, Ernesto A Roman, Nadine Alaimo, Mariana Gallo, Javier Santos
Human frataxin (FXN) is a highly conserved mitochondrial protein involved in iron homeostasis and activation of the iron-sulfur cluster assembly. FXN deficiency causes the neurodegenerative disease Friedreich's Ataxia. Here, we investigated the effect of alterations in loop-1, a stretch presumably essential for FXN function, on the conformational stability and dynamics of the native state. We generated four loop-1 variants, carrying substitutions, insertions and deletions. All of them were stable and well-folded proteins...
October 30, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29107586/ru-fe-bimetallic-complexes-synthesis-characterization-cytotoxicity-and-study-of-their-interactions-with-dna-hsa-and-human-topoisomerase-ib
#14
Jessica E Takarada, Adriana P M Guedes, Rodrigo S Correa, Elisângela de P Silveira-Lacerda, Silvia Castelli, Federico Iacovelli, Victor Marcelo Deflon, Alzir Azevedo Batista, Alessandro Desideri
Three ruthenium/iron-based compounds, 1: [Ru(MIm)(bipy)(dppf)]PF6 (MIm = 2-mercapto-1-methylimidazole anion), 2: [RuCl(Im)(bipy)(dppf)]PF6 (Im = imidazole), and 3: [Ru(tzdt)(bipy)(dppf)]PF6 (tzdt = 1,3-thiazolidine-2-thione anion) (dppf = 1,1'-bis(diphenylphosphine)ferrocene and bipy = 2,2'-bipyridine), were synthesized, and characterized by elemental analyses, conductivity, UV/Vis, IR, (1)H, (13)C and (31)P{1H} NMR spectroscopies, and by electrochemical technique. The complex 3 was also characterized by single-crystal X-ray...
October 28, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29111295/kinetic-and-structural-characterization-of-a-cis-3-chloroacrylic-acid-dehalogenase-homologue-in-pseudomonas-sp-uw4-a-potential-step-between-subgroups-in-the-tautomerase-superfamily
#15
Jake A LeVieux, Bert-Jan Baas, Tamer S Kaoud, Rebecca Davidson, Patricia C Babbitt, Yan Jessie Zhang, Christian P Whitman
A Pseudomonas sp. UW4 protein (UniProt K9NIA5) of unknown function was identified as similar to 4-oxalocrotonate tautomerase (4-OT)-like and cis-3-chloroacrylic acid dehalogenase (cis-CaaD)-like subgroups of the tautomerase superfamily (TSF). This protein lacks only Tyr-103 of the amino acids critical for cis-CaaD activity (Pro-1, His-28, Arg-70, Arg-73, Tyr-103, Glu-114). As it may represent an important variant of these enzymes, its kinetic and structural properties have been determined. The protein shows tautomerase activity with phenylenolpyruvate, but lacks native 4-OT activity and dehalogenase activity with the isomers of 3-chloroacrylic acid...
October 27, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29056417/regulation-of-glutamate-dehydrogenase-gdh-in-response-to-whole-body-freezing-in-wood-frog-liver-linked-to-differential-acetylation-and-adp-ribosylation
#16
Stuart R Green, Kenneth B Storey
Glutamate dehydrogenase (GDH) represents a critical enzyme catalyzing the reaction spanning amino acid catabolism, the Krebs cycle, and urea production in the wood frog. GDH breaks down glutamate and NAD(+) to generate α-ketoglutaric acid (α-KG), NADH and ammonium that can be metabolized to form urea. Purification of GDH from control and frozen male wood frog livers was performed using a two-step column chromatography procedure with a cation exchange column and a GTP-agarose affinity column. Analysis of kinetic parameters of the purified GDH showed several notable differences between the control and stress...
October 19, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29029877/substitutions-of-s101-decrease-proton-and-hydride-transfers-in-the-oxidation-of-betaine-aldehyde-by-choline-oxidase
#17
Giovanni Gadda, Hongling Yuan
Choline oxidase oxidizes choline to glycine betaine, with two flavin-mediated reactions to convert the alcohol substrate to the carbon acid product. Proton abstraction from choline or hydrated betaine aldehyde in the wild-type enzyme occurs in the mixing time of the stopped-flow spectrophotometer, thereby precluding a mechanistic investigation. Mutagenesis of S101 rendered the proton transfer reaction amenable to study. Here, we have investigated the aldehyde oxidation reaction catalyzed by the mutant enzymes using steady-state and rapid kinetics with betaine aldehyde...
October 10, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29024617/stm2360-encodes-a-d-ornithine-d-lysine-decarboxylase-in-salmonella-enterica-serovar-typhimurium
#18
Robert S Phillips, Pafe Poteh, Katherine A Miller, Timothy R Hoover
STM2360 is a gene located in a small operon of undetermined function in Salmonella enterica serovar Typhimurium LT2. The amino acid sequence of STM2360 shows significant similarity (∼30% identity) to diaminopimelate decarboxylase (DapDC), a Fold III pyridoxal-5'-phosphate (PLP) dependent enzyme involved in l-lysine biosynthesis. We have found that the protein coded by STM2360 has a previously undocumented catalytic activity, d-ornithine/d-lysine decarboxylase (DOKDC). The reaction products, cadaverine and putrescine, respectively, were identified by NMR and mass spectrometry...
October 9, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29017749/characterization-of-the-nuclear-import-pathway-for-blm-protein
#19
Zhiqiang Duan, Jiafu Zhao, Houqiang Xu, Haixu Xu, Xinqin Ji, Xiang Chen, Jianming Xiong
Numerous studies have shown that nuclear localization of BLM protein, a member of the RecQ helicases, mediated by nuclear localization signal (NLS) is critical for DNA recombination, replication and transcription, but the mechanism by which BLM protein is imported into the nucleus remains unknown. In this study, the nuclear import pathway for BLM was investigated. We found that nuclear import of BLM was inhibited by two dominant-negative mutants of importin β1 and NTF2/E42K, which lacks the ability to bind Ran and RanGDP, respectively, but was not inhibited by the Ran/Q69L, which is deficient in GTP hydrolysis...
October 7, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28986131/selenoprotein-msrb1-deficiency-exacerbates-acetaminophen-induced-hepatotoxicity-via-increased-oxidative-damage
#20
Ki Young Kim, Geun-Hee Kwak, Mahendra Pratap Singh, Vadim N Gladyshev, Hwa-Young Kim
Acetaminophen (APAP) overdose induces acute liver damage and failure via reactive oxygen species production and glutathione (GSH) depletion. Methionine sulfoxide reductase B1 (MsrB1) is an antioxidant selenoenzyme that specifically catalyzes the reduction of methionine R-sulfoxide residues. In this study, we used MsrB1 gene-knockout mice and primary hepatocytes to investigate the effect of MsrB1 on APAP-induced hepatotoxicity. Analyses of histological alterations and serum indicators of liver damage showed that MsrB1(-/-) mice were more susceptible to APAP-induced acute liver injury than wild-type (MsrB1(+/+)) mice...
October 3, 2017: Archives of Biochemistry and Biophysics
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