journal
MENU ▼
Read by QxMD icon Read
search

Archives of Biochemistry and Biophysics

journal
https://www.readbyqxmd.com/read/27932289/peroxiredoxin-6-in-the-repair-of-peroxidized-cell-membranes-and-cell-signaling
#1
REVIEW
Aron B Fisher
Peroxiredoxin 6 represents a widely distributed group of peroxiredoxins that contain a single conserved cysteine in the protein monomer (1-cys Prdx). The cys when oxidized to the sulfenic form is reduced with glutathione (GSH) catalyzed by the π isoform of GSH-S-transferase. Three enzymatic activities of the protein have been described:1) peroxidase with H2O2, short chain hydroperoxides and phospholipid hydroperoxides as substrates; 2) phospholipase A2 (PLA2); and 3) lysophosphatidylcholine acyl transferase (LPCAT)...
December 5, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27916505/cyclophilin-d-over-expression-increases-mitochondrial-complex-iii-activity-and-accelerates-supercomplex-formation
#2
Julie C Etzler, Mariana Bollo, Deborah Holstein, Janice Jianhong Deng, Viviana Perez, Da-Ting Lin, Arlan Richardson, Yidong Bai, James D Lechleiter
Cyclophilin D (CyPD), a mitochondrial matrix protein, has been widely studied for its role in mitochondrial-mediated cell death. Unexpectedly, we previously discovered that overexpression of CyPD in a stable cell line, increased mitochondrial membrane potentials and enhanced cell survival under conditions of oxidative stress. Here, we investigated the underlying mechanisms responsible for these findings. Spectrophotometric measurements in isolated mitochondria revealed that overexpression of CyPD in HEK293 cells increased respiratory chain activity, but only for Complex III (CIII)...
December 1, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27889386/protein-disulfide-isomerases-redox-connections-in-and-out-of-the-endoplasmic-reticulum
#3
Ana Iochabel Soares Moretti, Francisco Rafael Martins Laurindo
Protein disulfide isomerases are thiol oxidoreductase chaperones from thioredoxin superfamily. As redox folding catalysts from the endoplasmic reticulum (ER), their roles in ER-related redox homeostasis and signaling are well-studied. PDIA1 exerts thiol oxidation/reduction and isomerization, plus chaperone effects. Also, substantial evidence indicates that PDIs regulate thiol-disulfide switches in other cell locations such as cell surface and possibly cytosol. Subcellular PDI translocation routes remain unclear and seem Golgi-independent...
November 24, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27884599/influence-of-sequence-and-lipid-type-on-membrane-perturbation-by-human-and-rat-amyloid-%C3%AE-peptide-1-42
#4
Anne M Brown, David R Bevan
The hallmark characteristics of plaque formation and neuronal cell death in Alzheimer's disease (AD) are caused principally by the amyloid β-peptide (Aβ). Aβ sequence and lipid composition are essential variables to consider when elucidating the impact of biological membranes on Aβ structure and the effect of Aβ on membrane integrity. Atomistic molecular dynamics simulations testing two Aβ sequences, human and rat Aβ (HAβ and RAβ, respectively), and five lipid types were performed to assess the effect of these variables on membrane perturbation and potential link to AD phenotype differences based on differences in sequence...
November 21, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27864141/long-term-inhibition-of-cyclophilin-d-results-in-intracellular-translocation-of-calcein-am-from-mitochondria-to-lysosomes
#5
Daisuke Shinohe, Asuka Kobayashi, Marina Gotoh, Kotaro Tanaka, Yoshihiro Ohta
Cyclophilin D is a peptidyl-prolyl cis-trans isomerase localized in the mitochondrial matrix. Although its effects on mitochondrial characteristics have been well studied, its relation to the uptake of molecules by mitochondria remains unknown. Here, we demonstrated the effects of cyclophilin D on the intracellular translocation of calcein AM. Following addition of calcein AM to control cells or cells overexpressing wild-type cyclophilin D, calcein fluorescence was observed in mitochondria. However, long-term inhibition of cyclophilin D in these cells altered the localization of calcein fluorescence from mitochondria to lysosomes without changing mitochondrial esterase activity...
November 15, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27840097/structural-and-functional-studies-of-the-leishmania-braziliensis-mitochondrial-hsp70-similarities-and-dissimilarities-to-human-orthologues
#6
Paulo R Dores-Silva, Letícia S Nishimura, Vanessa T R Kiraly, Júlio C Borges
Heat shock protein 70 kDa (Hsp70) is a conserved molecular chaperone family involved in several functions related to protein homeostasis. In eukaryotes, Hsp70 homologues are found in all cell compartments. The mitochondrial Hsp70 isoform (mtHsp70) is involved in import of mitochondrial matrix proteins as well as their folding and maturation. Moreover, mtHsp70 has the propensity to self-aggregate, and it depends on the action of the co-chaperone Hsp70-escort protein 1 (Hep1) to be produced functional. Here, we analyze the solution structure and function of mtHsp70 of Leishmania braziliensis (LbmtHsp70)...
November 11, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27840096/signaling-by-4-hydroxy-2-nonenal-exposure-protocols-target-selectivity-and-degradation
#7
Hongqiao Zhang, Henry Jay Forman
4-hydroxy-2-nonenal (HNE), a major non-saturated aldehyde product of lipid peroxidation, has been extensively studied as a signaling messenger. In these studies a wide range of HNE concentrations have been used, ranging from the unstressed plasma concentration to far beyond what would be found in actual pathophysiological condition. In addition, accumulating evidence suggest that signaling protein modification by HNE is specific with only those proteins with cysteine, histidine, and lysine residues located in certain sequence or environments adducted by HNE...
November 10, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27823907/impact-of-hypoxia-inducible-factors-on-estrogen-receptor-expression-in-breast-cancer-cells
#8
Matthias Wolff, Friederike Katharina Kosyna, Jürgen Dunst, Wolfgang Jelkmann, Reinhard Depping
In women breast cancer is still the most commonly diagnosed cancer. This type of cancer is classified as a hormone-dependent tumor. Estrogen receptor (ER) expression and functional status contribute to breast cancer development and progression. Another important factor associated with cancer is hypoxia which is defined as the state of reduced oxygen availability in tissues. Intratumoral hypoxia results in the activation of the hypoxia inducible factors (HIFs). HIFs are heterodimeric transcription factors involved in the regulation of many cellular processes, such as angiogenesis, anaerobic metabolism, cell proliferation/survival, and promotion of metastasis...
November 5, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27818203/cytotoxicity-of-prion-protein-derived-cell-penetrating-peptides-is-modulated-by-ph-but-independent-of-amyloid-formation
#9
Vineeth Mukundan, Christy Maksoudian, Maria C Vogel, Ibrahim Chehade, Marios S Katsiotis, Saeed M Alhassan, Mazin Magzoub
Prion diseases are associated with conversion of cellular prion protein (PrP(C)) into an abnormally folded and infectious scrapie isoform (PrP(Sc)). We previously showed that peptides derived from the unprocessed N-termini of mouse and bovine prion proteins, mPrP1-28 and bPrP1-30, function as cell-penetrating peptides (CPPs), and destabilize model membrane systems, which could explain the infectivity and toxicity of prion diseases. However, subsequent studies revealed that treatment with mPrP1-28 or bPrP1-30 significantly reduce PrP(Sc) levels in prion-infected cells...
November 3, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27815088/-13-c-kinetic-isotope-effects-on-the-reaction-of-a-flavin-amine-oxidase-determined-from-whole-molecule-isotope-effects
#10
José R Tormos, Marina B Suarez, Paul F Fitzpatrick
A large number of flavoproteins catalyze the oxidation of amines. Because of the importance of these enzymes in metabolism, their mechanisms have previously been studied using deuterium, nitrogen, and solvent isotope effects. While these results have been valuable for computational studies to distinguish among proposed mechanisms, a measure of the change at the reacting carbon has been lacking. We describe here the measurement of a (13)C kinetic isotope effect for a representative amine oxidase, polyamine oxidase...
November 1, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27794428/how-are-nitrosothiols-formed-de-novo-in%C3%A2-vivo
#11
REVIEW
Jack R Lancaster
The biological mechanisms of de novo formation of cellular nitrosothiols (as opposed to transnitrosation) are reviewed. The approach is to introduce chemical foundations for each mechanism, followed by evidence in biological systems. The general categories include mechanisms involving nitrous acid, NO autoxidation and oxidant stress, redox active and inactive metal ions, and sulfide/persulfide. Important conclusions/speculations are that de novo cellular thiol nitrosation (1) is an oxidative process, and so should be considered within the family of other thiol oxidative modifications, (2) may not involve a single dominant process but depends on the specific conditions, (3) does not involve O2 under at least some conditions, and (4) may serve to provide a "substrate pool" of protein cysteine nitrosothiol which could, through subsequent enzymatic transnitrosation/denitrosation, be "rearranged" to accomplish the specificity and regulatory control required for effective post-translational signaling...
October 26, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27789205/repositioning-nordihydroguaiaretic-acid-as-a-potent-inhibitor-of-systemic-amyloidosis-and-associated-cellular-toxicity
#12
Saima Nusrat, Nida Zaidi, Masihuz Zaman, Sehbanul Islam, Mohammad Rehan Ajmal, Mohammad Khursheed Siddiqi, Manas Kumar Santra, Rizwan Hasan Khan
Although the cure of amyloid related neurodegenerative diseases, non-neuropathic amyloidogenic diseases and non-neuropathic systemic amyloidosis are appealing energetic research attempts, beneficial medication is still to be discovered. There is a need to explore intensely stable therapeutic compounds, potent enough to restrict, disrupt or wipe out such toxic aggregates. We had performed a comprehensive biophysical, computational and cell based assay, that shows Nordihydroguaiaretic acid (NA) not only significantly inhibits heat induced hen egg white lysozyme (HEWL) fibrillation but also disaggregates preformed HEWL fibrils and reduces the cytoxicity of amyloid fibrils as well as disaggregated fibrillar species...
October 24, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27789204/neutrophils-recruited-to-the-myocardium-after-acute-experimental-myocardial-infarct-generate-hypochlorous-acid-that-oxidizes-cardiac-myoglobin
#13
Xiao Suo Wang, Hyun Bo Kim, Andrea Szuchman-Sapir, Aisling McMahon, Joanne M Dennis, Paul K Witting
Myocardial inflammation following acute myocardial infarct (AMI) is associated with risk of congestive heart failure. Pro-inflammatory neutrophils were recruited to the damaged myocardium 24 h after permanent coronary ligation in rats to induce AMI as judged by the presence of immune-positive myeloperoxidase (MPO) in the tissues; MPO generates the oxidant hypochlorous acid (HOCl). Neutrophils were absent in hearts from Control (untreated) and surgical Sham. Similarly, rats exposed to 1 h coronary ligation (Ischemia) showed no neutrophil infiltrate...
October 24, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27771300/an-insight-into-fusion-technology-aiding-efficient-recombinant-protein-production-for-functional-proteomics
#14
REVIEW
Dinesh K Yadav, Neelam Yadav, Sarika Yadav, Shafiul Haque, Narendra Tuteja
Advancements in peptide fusion technologies to maximize the protein production has taken a big leap to fulfill the demands of post-genomics era targeting elucidation of structure/function of the proteome and its therapeutic applications, by over-expression in heterologous expression systems. Despite being most preferred protein expression system armed with variety of cardinal fusion tags, expression of the functionally active recombinant protein in E. coli remains plagued. The present review critically analyses the aptness of well-characterized fusion tags utilized for over-expression of recombinant proteins with improved solubility and their compatibility with downstream purification procedures...
October 19, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27769838/keap1-as-the-redox-sensor-of-the-antioxidant-response
#15
Virve Sihvola, Anna-Liisa Levonen
Reactive oxygen species (ROS) and products of their reactions with cellular macromolecules such as unsaturated fatty acids have been implicated to be important regulators of signalling processes via oxidation or alkylation of redox active thiol residues in target proteins. One of key redox-sensitive signalling proteins mediating the response to oxidant stress is Keap1 (Kelch-like erythroid cell-derived protein with CNC homology [ECH]-associated protein 1), which is a negative regulator of transcription factor Nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) and the central hub for sensing endogenous and environmental oxidative and electrophilic stress...
October 18, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27769837/ligand-binding-phenomena-that-pertain-to-the-metabolic-function-of-renalase
#16
Brett A Beaupre, Joseph V Roman, Matthew R Hoag, Kathleen M Meneely, Nicholas R Silvaggi, Audrey L Lamb, Graham R Moran
Renalase catalyzes the oxidation of isomers of β-NAD(P)H that carry the hydride in the 2 or 6 positions of the nicotinamide base to form β-NAD(P)(+). This activity is thought to alleviate inhibition of multiple β-NAD(P)-dependent enzymes of primary and secondary metabolism by these isomers. Here we present evidence for a variety of ligand binding phenomena relevant to the function of renalase. We offer evidence of the potential for primary metabolism inhibition with structures of malate dehydrogenase and lactate dehydrogenase bound to the 6-dihydroNAD isomer...
October 18, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27756681/the-role-of-peroxiredoxin-1-in-redox-sensing-and-transducing
#17
Elizabeth C Ledgerwood, James W A Marshall, Johannes F Weijman
Peroxiredoxin 1 is a member of the ubiquitous peroxiredoxin family of thiol peroxidases that catalyse the reduction of peroxides. In recent years eukaryotic peroxiredoxins have emerged as a critical component of cellular redox signalling, particularly in response to alterations in production of hydrogen peroxide. Peroxiredoxins are exquisitely sensitive to oxidation by hydrogen peroxide making them key peroxide sensing enzymes within cells. Evidence gathered over the last decade suggests that in addition to sensing the redox signal, peroxiredoxins have a major role in transducing this signal to downstream signalling proteins, ultimately contributing to regulation of diverse cellular processes including proliferation, differentiation and apoptosis...
October 15, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27756680/steady-state-kinetic-studies-reveal-that-the-anti-cancer-target-ubiquitin-specific-protease-17-usp17-is-a-highly-efficient-deubiquitinating-enzyme
#18
Nicole M Hjortland, Andrew D Mesecar
USP17 is a deubiquitinating enzyme that is upregulated in numerous cancers and therefore a drug target. We developed a robust expression, purification, and assay system for USP17 enabling its enzymatic and structural characterization. USP17 was expressed in E. coli as inclusion bodies and then solubilized, refolded, and purified using affinity and size-exclusion chromatography. Milligram quantities of pure USP17 can be produced that is catalytically more efficient (kcat/Km = 1500 (x10(3)) M(-1)sec(-1)) than other human USPs studied to date...
October 15, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27746119/development-of-the-first-internally-quenched-fluorescent-substrates-of-human-cathepsin-c-the-application-in-the-enzyme-detection-in-biological-samples
#19
Monika Łęgowska, Yveline Hamon, Anna Wojtysiak, Renata Grzywa, Marcin Sieńczyk, Timo Burster, Brice Korkmaz, Adam Lesner
Cathepsin C is a widely expressed cysteine exopeptidase that is mostly recognized for the activation of the granule-associated proinflammatory serine proteases in neutrophils, cytotoxic T lymphocytes and mast cells. It has been shown that the enzyme can be secreted extracellularly; however, its occurrence in human bodily fluids/physiological samples has not been thoroughly studied. In the course of this study, the first fluorescence resonance energy transfer peptides for the measurement of the activity of human cathepsin C were designed and synthesized...
October 13, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27720685/insight-into-the-impact-of-two-structural-calcium-ions-on-the-properties-of-pleurotus-eryngii-versatile-ligninolytic-peroxidase
#20
Yu Gao, Lanyan Zheng, Jian-Jun Li, Yuguang Du
Two structural Ca(2+) (proximal and distal) is known to be important for ligninolytic peroxidases. However, few studies toward impact of residues involved in two Ca(2+) on properties of ligninolytic peroxidases have been done, especially the proximal one. In this study, mutants of nine residues involved in liganding two Ca(2+) of Pleurotus eryngii versatile peroxidase (VP) were investigated. Most mutants almost completely lost activities, except the mutants of proximal Ca(2+) - S170A and V192T. In comparison with WT (wild type), optimal pH values of S170A, S170D, and V192T shifted from pH 3...
October 5, 2016: Archives of Biochemistry and Biophysics
journal
journal
23469
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"