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https://www.readbyqxmd.com/read/28204905/transcriptome-assembly-and-identification-of-genes-and-snps-associated-with-growth-traits-in-largemouth-bass-micropterus-salmoides
#1
Shengjie Li, Hao Liu, Junjie Bai, Xinping Zhu
Growth is one of the most crucial economic traits of all aquaculture species, but the molecular mechanisms involved in growth of largemouth bass (Micropterus salmoides) are poorly understood. The objective of this study was to screen growth-related genes of M. salmoides by RNA sequencing and identify growth-related single-nucleotide polymorphism (SNP) markers through a growth association study. The muscle transcriptomes of fast- and slow-growing largemouth bass were obtained using the RNA-Seq technique. A total of 54,058,178 and 54,742,444 qualified Illumina read pairs were obtained for the fast-growing and slow-growing groups, respectively, giving rise to 4,865,236,020 and 4,926,819,960 total clean bases, respectively...
February 15, 2017: Genetica
https://www.readbyqxmd.com/read/28185042/phylogenomic-relationship-of-feijoa-acca-sellowiana-o-berg-burret-with-other-myrtaceae-based-on-complete-chloroplast-genome-sequences
#2
Lilian Oliveira de Machado, Leila do Nascimento Vieira, Valdir Marcos Stefenon, Fábio de Oliveira Pedrosa, Emanuel Maltempi de Souza, Miguel Pedro Guerra, Rubens Onofre Nodari
Given their distribution, importance, and richness, Myrtaceae species comprise a model system for studying the evolution of tropical plant diversity. In addition, chloroplast (cp) genome sequencing is an efficient tool for phylogenetic relationship studies. Feijoa [Acca sellowiana (O. Berg) Burret; CN: pineapple-guava] is a Myrtaceae species that occurs naturally in southern Brazil and northern Uruguay. Feijoa is known for its exquisite perfume and flavorful fruits, pharmacological properties, ornamental value and increasing economic relevance...
February 9, 2017: Genetica
https://www.readbyqxmd.com/read/28161823/phylogeography-of-littorina-sitkana-in-the-northwestern-pacific-ocean-evidence-of-eastward-trans-pacific-colonization-after-the-last-glacial-maximum
#3
Noriko Azuma, Nadezhda I Zaslavskaya, Tomoyasu Yamazaki, Takahiro Nobetsu, Susumu Chiba
We investigated genetic diversity and population structure of the Sitka periwinkle Littorina sitkana along the coastlines of the northwestern Pacific (NWP) to evaluate the possibility of trans-Pacific colonization of this species from the NWP to the northeastern Pacific (NEP) after the Last Glacial Maximum. We sampled L. sitkana from 32 populations in the NWP, and sequenced a region of the mitochondrial cytochrome b oxidase gene for population genetic analyses. The results were compared with those of previous reports from the NEP...
February 4, 2017: Genetica
https://www.readbyqxmd.com/read/28160169/complete-mitochondrial-genome-from-south-american-catfish-pseudoplatystoma-reticulatum-eigenmann-eigenmann-and-its-impact-in-siluriformes-phylogenetic-tree
#4
Luciana Cristine Vasques Villela, Anderson Luis Alves, Eduardo Sousa Varela, Michel Eduardo Beleza Yamagishi, Poliana Fernanda Giachetto, Naiara Milagres Augusto da Silva, Josi Margarete Ponzetto, Samuel Rezende Paiva, Alexandre Rodrigues Caetano
The cachara (Pseudoplatystoma reticulatum) is a Neotropical freshwater catfish from family Pimelodidae (Siluriformes) native to Brazil. The species is of relative economic importance for local aquaculture production and basic biological information is under development to help boost efforts to domesticate and raise the species in commercial systems. The complete cachara mitochondrial genome was obtained by assembling Illumina RNA-seq data from pooled samples. The full mitogenome was found to be 16,576 bp in length, showing the same basic structure, order, and genetic organization observed in other Pimelodidae, with 13 protein-coding genes, 2 rNA genes, 22 trNAs, and a control region...
February 3, 2017: Genetica
https://www.readbyqxmd.com/read/28160168/cystic-fibrosis-in-afro-brazilians-xk-haplotypes-analysis-supports-the-european-origin-of-p-f508del-mutation
#5
D A S de Souza, F R Faucz, R B de Alexandre, M A Santana, E L S de Souza, F J C Reis, L Pereira-Ferrari, V S Sotomaior, L Culpi, J A Phillips, S Raskin
Cystic fibrosis (CF) is a common autosomal recessive disorder, being the p.F508del the most frequent mutation. Also, a nearby restriction fragment length polymorphism (RFLP) named XK (KM19 and XV2C) is non-randomly associated with specific CF alleles. Our aim was to analyze the occurrence of the p.F508del mutation and XK haplotypes in Afro-Brazilians CF patients and controls, since these data is available for the other two main ethnic groups found in Brazil (Euro-Brazilians and Brazilian Amerindians), contributing for the whole comprehension of these haplotypes in the Brazilian population...
February 3, 2017: Genetica
https://www.readbyqxmd.com/read/28154959/differential-sexual-survival-of-drosophila-melanogaster-on-copper-sulfate
#6
Michael A Balinski, Ronny C Woodruff
Based on studies of the influence of X-chromosomes on the viability of Drosophila melanogaster exposed to cadmium, and on the role of X-linked genes on copper homeostasis, we examined the effect of copper sulfate (CuSO4) on offspring viability using three independent, inbred D. melanogaster crosses (ensuring identical autosomes for males and females within each cross). Each cross was performed with attached X-chromosome females and males with a single X-chromosome. As female D. melanogaster have less metallothionein RNA expression than males, we predicted fewer female offspring than male offspring in crosses exposed to CuSO4, even though females have two copies of X-chromosome genes, possibly resulting in overdominant heterozygosity...
February 2, 2017: Genetica
https://www.readbyqxmd.com/read/28120213/genetic-characterization-of-residual-triatoma-infestans-populations-from-brazil-by-microsatellite
#7
Carlota Josefovicz Belisário, Grasielle Caldas D'Avila Pessoa, Eduardo Melos Silva, Aline Cristine Luiz Rosa, Rafaela Elias Ferreira, Cleonara Bedin, Tania Wilhelms, Fernanda de Mello, Helder Silveira Coutinho, Eduardo Lins Oyama Fonseca, Roberto Fonseca Dos Santos, Vera Lucia Cortiço Corrêa Rodrigues, João Carlos Pinto Dias, Liléia Diotaiuti
In spite of long-term efforts to eliminate Triatoma infestans (Klug 1834) from Brazil, residual foci still persist in the states of Bahia and Rio Grande do Sul. Data on the genetic variability and structuring of these populations are however lacking. Using nine microsatellite loci, we characterized one residual T. infestans population from Bahia and four from Rio Grande do Sul, and compared them with bugs originally from an older focus in São Paulo; 224 bugs were genotyped. The number of alleles per locus ranged from 5 to 11...
January 24, 2017: Genetica
https://www.readbyqxmd.com/read/28108874/surviving-in-isolation-genetic-variation-bottlenecks-and-reproductive-strategies-in-the-canarian-endemic-limonium-macrophyllum-plumbaginaceae
#8
Ares Jiménez, Barbara Weigelt, Arnoldo Santos-Guerra, Juli Caujapé-Castells, José María Fernández-Palacios, Elena Conti
Oceanic archipelagos are typically rich in endemic taxa, because they offer ideal conditions for diversification and speciation in isolation. One of the most remarkable evolutionary radiations on the Canary Islands comprises the 16 species included in Limonium subsection Nobiles, all of which are subject to diverse threats, and legally protected. Since many of them are single-island endemics limited to one or a few populations, there exists a risk that a loss of genetic variation might limit their long-term survival...
January 20, 2017: Genetica
https://www.readbyqxmd.com/read/28093669/genetic-diversity-and-population-structure-of-anastrepha-striata-diptera-tephritidae-in-three-natural-regions-of-southwestern-colombia-using-mitochondrial-sequences
#9
Jenny Johana Gallo-Franco, Sandra Marcela Velasco-Cuervo, Elkin Aguirre-Ramirez, Ranulfo González Obando, Nancy Soraya Carrejo, Nelson Toro-Perea
Anastrepha striata is widely distributed across the Americas and is a pest of economically important crops, especially crops of the Myrtaceae family. Insect population structures can be influenced by the presence of physical barriers or characteristics associated with habitat differences. This study evaluated the effect of the Western Andes on the population structure of A. striata. Individuals were collected from Psidium guajava fruits from three natural regions of southwestern Colombia (Pacific Coast, mountainous region and the inter-Andean valley of the Cauca River)...
January 16, 2017: Genetica
https://www.readbyqxmd.com/read/28093668/molecular-characterization-of-hoxc8-gene-and-methylation-status-analysis-of-its-exon-1-associated-with-the-length-of-cashmere-fiber-in-liaoning-cashmere-goat
#10
Wen L Bai, Jiao J Wang, Rong H Yin, Yun L Dang, Ze Y Wang, Yu B Zhu, Yu Y Cong, Liang Deng, Dan Guo, Shi Q Wang, Shu H Yang, Hui L Xue
Homeobox protein Hox-C8 (HOXC8) is a member of Hox family. It is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. In the present study, we isolated and characterized a full-length open reading frame of HOXC8 cDNA from the skin tissue of Liaoning cashmere goat, as well as, established a phylogenetic relationship of goat HOXC8 with that of other species. Also, we investigated the effect of methylation status of HOXC8 exon 1 at anagen secondary hair follicle on the cashmere fiber traits in Liaoning cashmere goat...
January 16, 2017: Genetica
https://www.readbyqxmd.com/read/28070759/qtls-for-heading-date-and-plant-height-under-multiple-environments-in-rice
#11
Zhongmin Han, Wei Hu, Cong Tan, Yongzhong Xing
Both heading date and plant height are important traits related to grain yield in rice. In this study, a recombinant inbred lines (RILs) population was used to map quantitative trait loci (QTLs) for both traits under 3 long-day (LD) environments and 1 short-day (SD) environment. A total of eight QTLs for heading date and three QTLs for plant height were detected by composite interval mapping under LD conditions. Additional one QTL for heading date and three QTLs for plant height were identified by Two-QTL model under LD conditions...
January 9, 2017: Genetica
https://www.readbyqxmd.com/read/28070758/phylogeography-of-the-asian-rice-gall-midge-orseolia-oryzae-wood-mason-diptera-cecidomyiidae-in-thailand
#12
Solene Janique, Wantana Sriratanasak, Kulchana Ketsuwan, Jirapong Jairin, Ekgachai Jeratthitikul
The Asian rice gall midge (RGM) Orseolia oryzae (Wood Mason) (Diptera: Cecidomyiidae) is a major pest of rice, leading to yield losses in Thailand and many Asian countries. Despite an increasing number of reported midge outbreaks and the presence of many susceptible rice varieties, only a few studies have focused on the genetic variation of the midges. Therefore, we analyzed the phylogeography among Thai RGM populations covering north, northeast and central Thailand. Two mitochondrial DNA genes, cytochrome C oxidase I (COI) and 12S, and a non-coding repeat region (RR) situated just before COI were amplified...
January 9, 2017: Genetica
https://www.readbyqxmd.com/read/27995348/mitochondrial-genome-of-pteronotus-personatus-chiroptera-mormoopidae-comparison-with-selected-bats-and-phylogenetic-considerations
#13
Ricardo López-Wilchis, Miguel Ángel Del Río-Portilla, Luis Manuel Guevara-Chumacero
We described the complete mitochondrial genome (mitogenome) of the Wagner's mustached bat, Pteronotus personatus, a species belonging to the family Mormoopidae, and compared it with other published mitogenomes of bats (Chiroptera). The mitogenome of P. personatus was 16,570 bp long and contained a typically conserved structure including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region (D-loop). Most of the genes were encoded on the H-strand, except for eight tRNA and the ND6 genes...
December 19, 2016: Genetica
https://www.readbyqxmd.com/read/27909948/little-effect-of-hsp90-inhibition-on-the-quantitative-wing-traits-variation-in-drosophila-melanogaster
#14
Kazuo H Takahashi
Drosophila wings have been a model system to study the effect of HSP90 on quantitative trait variation. The effect of HSP90 inhibition on environmental buffering of wing morphology varies among studies while the genetic buffering effect of it was examined in only one study and was not detected. Variable results so far might show that the genetic background influences the environmental and genetic buffering effect of HSP90. In the previous studies, the number of the genetic backgrounds used is limited. To examine the effect of HSP90 inhibition with a larger number of genetic backgrounds than the previous studies, 20 wild-type strains of Drosophila melanogaster were used in this study...
December 1, 2016: Genetica
https://www.readbyqxmd.com/read/27904978/development-and-characterization-of-twelve-microsatellite-markers-for-porphyra-linearis-greville
#15
Elena Varela-Álvarez, Cristina Paulino, Ester A Serrão
The genus Porphyra (and its sister genus Pyropia) contains important red algal species that are cultivated and/or harvested for human consumption, sustaining a billion-dollar aquaculture industry. A vast amount of research has been focused on species of this genus, including studies on genetics and genomics among other areas. Twelve novel microsatellite markers were developed here for Porphyra linearis. Markers were characterized using 32 individuals collected from four natural populations of P. linearis with total heterozygosity varying from 0...
November 30, 2016: Genetica
https://www.readbyqxmd.com/read/27838803/evidence-of-birth-and-death-evolution-of-5s-rrna-gene-in-channa-species-teleostei-perciformes
#16
Anindya Sundar Barman, Mamta Singh, Rajeev Kumar Singh, Kuldeep Kumar Lal
In higher eukaryotes, minor rDNA family codes for 5S rRNA that is arranged in tandem arrays and comprises of a highly conserved 120 bp long coding sequence with a variable non-transcribed spacer (NTS). Initially the 5S rDNA repeats are considered to be evolved by the process of concerted evolution. But some recent reports, including teleost fishes suggested that evolution of 5S rDNA repeat does not fit into the concerted evolution model and evolution of 5S rDNA family may be explained by a birth-and-death evolution model...
December 2016: Genetica
https://www.readbyqxmd.com/read/27832462/influence-of-environmental-heterogeneity-on-the-distribution-and-persistence-of-a-subterranean-rodent-in-a-highly-unstable-landscape
#17
María Jimena Gómez Fernández, Emma S M Boston, Oscar E Gaggiotti, Marcelo J Kittlein, Patricia M Mirol
In this study we combine information from landscape characteristics, demographic inference and species distribution modelling to identify environmental factors that shape the genetic distribution of the fossorial rodent Ctenomys. We sequenced the mtDNA control region and amplified 12 microsatellites from 27 populations distributed across the Iberá wetland ecosystem. Hierarchical Bayesian modelling was used to construct phylogenies and estimate divergence times. We developed species distribution models to determine what climatic variables and soil parameters predicted species presence by comparing the current to the historic and predicted future distribution of the species...
December 2016: Genetica
https://www.readbyqxmd.com/read/27822711/the-complete-mitogenome-of-a-jumping-spider-carrhotus-xanthogramma-araneae-salticidae-and-comparative-analysis-in-four-salticid-mitogenomes
#18
COMPARATIVE STUDY
Wen-Yuan Fang, Zheng-Liang Wang, Chao Li, Xiao-Qing Yang, Xiao-Ping Yu
The complete mitogenome of the jumping spider Carrhotus xanthogramma was determined and comparative analysis among four salticid mitogenomes was conducted. The circular genome is 14,563 bp in size and contains a complete set of genes that usually present in the metazoa. All of the 13 protein-coding genes begin with a typical ATN codon and stop with the canonical stop codons, except for ND4 and ND4L genes with an incomplete stop codon T. All of the tRNAs cannot be formed the fully paired acceptor stems and seven out of them cannot be folded into the typical cloverleaf-shaped secondary structures...
December 2016: Genetica
https://www.readbyqxmd.com/read/27796528/identification-of-critical-amino-acid-residues-and-functional-conservation-of-the-neurospora-crassa-and-rattus-norvegicus-orthologues-of-neuronal-calcium-sensor-1
#19
Dibakar Gohain, Rekha Deka, Ranjan Tamuli
Neuronal calcium sensor-1 (NCS-1) is a member of neuronal calcium sensor family of proteins consisting of an amino terminal myristoylation domain and four conserved calcium (Ca(2+)) binding EF-hand domains. We performed site-directed mutational analysis of three key amino acid residues that are glycine in the conserved site for the N-terminal myristoylation, a conserved glutamic acid residue responsible for Ca(2+) binding in the third EF-hand (EF3), and an unusual non-conserved amino acid arginine at position 175 in the Neurospora crassa NCS-1...
December 2016: Genetica
https://www.readbyqxmd.com/read/27796527/genetic-structure-and-diversity-in-an-isolated-population-of-an-endemic-mole-salamander-ambystoma-rivulare-taylor-1940-of-central-mexico
#20
Rosa-Laura Heredia-Bobadilla, Octavio Monroy-Vilchis, Martha M Zarco-González, Daniel Martínez-Gómez, Germán David Mendoza-Martínez, Armando Sunny
Human activities are affecting the distribution of species worldwide by causing fragmentation and isolation of populations. Isolation and fragmentation lead to populations with lower genetic variability and an increased chance of inbreeding and genetic drift, which results in a loss of biological fitness over time. Studies of the genetic structure of small and isolated populations are critically important for management and conservation decisions. Ambystoma rivulare is a micro-endemic Mexican mole salamander from central Mexico...
December 2016: Genetica
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