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Experimental Parasitology

João Victor Facchini Rodrigues, Fabio Ribeiro Braga, Artur Kanadani Campos, Lorendane Millena de Carvalho, Juliana Milani Araujo, Anderson Rocha Aguiar, Carolina Magri Ferraz, Wendeo Ferreira da Silveira, Marisa Caixetta Valadão, Thais de Oliveira, Samuel Galvão de Freitas, Jackson Victor de Araújo
Three experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation...
November 10, 2017: Experimental Parasitology
Ferdaous Albouchi, Ines Sifaoui, Maria Reyes-Batlle, Atteneri López-Arencibia, José E Piñero, Jacob Lorenzo-Morales, Manef Abderrabba
Acanthamoeba infections cause serious humans diseases, such as amoebic keratitis and granulomatous amoebic encephalitis. Melaleuca essential oil has been reported to be effective in treating bacterial and fungal infections. However, the anti-parasitic effects of this essential oil are not well studied. In the present study, we first characterized the composition of the essential oil, extracted from the Tunisian Melaleuca styphelioides leaves, and then tested its effect on the Acanthamoeba castellanii Neff. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the major common compounds were Caryophyllene oxide (23...
November 9, 2017: Experimental Parasitology
Masahiro Yamasaki, Nao Watanabe, Natsuki Idaka, Tohru Yamamori, Ken-Ichi Otsuguro, Naohiro Uchida, Aiko Iguchi, Hiroshi Ohta, Mitsuyoshi Takiguchi
The mechanism of the development of diminazene aceturate (DA) resistance in Babesia gibsoni is still unknown even though DA-resistant B. gibsoni isolate was previously developed in vitro. To clarify the mechanisms of DA-resistance in B. gibsoni, we initially examined the intracellular DA content in the DA-resistant isolate using high-performance liquid chromatography, and compared it with that in the wild-type. As a result, the intracellular DA content in the DA-resistant isolate was significantly lower than that in the wild-type, suggesting that the decreased DA content may contribute to DA-resistance...
November 6, 2017: Experimental Parasitology
Marina Patsyuk
Two parasitic amoebian species are found in mollusks of the water bodies of Ukraine. Vahlkampfia sp. is found in hepatopankreas of Unio conus Spengler, 1793, and Acanthamoeba sp. is observed in mantle cavity of Viviparus viviparus Linnaeus, 1758. For these protist species, the mollusks are shown to be intermediate hosts where amoebae feed and reproduce. An experimental infection with Vahlkampfia sp. and Acanthamoeba sp. was not successful, no pathological changes in mollusks were observed. These amoebae are successfully cultured in fresh water and agar medium, hence we can safely consider them free-living...
November 2, 2017: Experimental Parasitology
Olfa Chiboub, Leila Ktari, Ines Sifaoui, Atteneri López-Arencibia, Maria Reyes-Batlle, Mondher Mejri, Basilio Valladares, Manef Abderrabba, José E Piñero, Jacob Lorenzo-Morales
Free-living amoebae of genus Acanthamoeba are opportunistic pathogens widely distributed in the environment, and are the causative agents of several humans' infections, such as Acanthamoeba keratitis, Granulomatous Amoebic Encephalitis and also disseminated infections. The existence of the cyst stage complicates Acanthamoeba therapy as it is highly resistant to antibiotics and physical agents. All these facts reinforced the necessity to find and develop an effective therapy against Acanthamoeba infections. In the present study, we are interested to several seaweeds species collected from the Tunisian coasts and belonging to the 3 phyla (brown, green and red algae)...
November 2, 2017: Experimental Parasitology
Najet Souhaiel, Ines Sifaoui, Dorsaf Ben Hassine, Jean Bleton, Myriam Bonose, Fathi Moussa, José E Piñero, Jacob Lorenzo-Morales, Manef Abderrabba
Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Essential Oils (EO) obtained from leaves and flowers and aerial parts of Ammoides pusilla by an alternative method "Hydrodistillation''. Identification and quantification were realized by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detection (GC-FID)...
November 2, 2017: Experimental Parasitology
Catalina Flores-Maldonado, Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Juan Manuel Gallardo, Mónica González-Lázaro, Verónica Ivonne Hernández-Ramírez, Patricia Talamás-Rohana, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
Free-living amoebae of the genus Acanthamoeba are protozoa ubiquitously found in nature. Some species of the genus are potentially pathogenic for humans provoking keratitis in healthy individuals, often in contact lens wearers and opportunistic infections such as pneumonitis, fatal granulomatous encephalitis and skin infections, particularly in immunocompromised individuals. The pathogenic mechanisms of these amoebae are poorly understood, however it had been suggested that contact dependent mechanisms are important during invasion, regardless of the epithelia type, since amoebae penetrate epithelia separating tight junction (TJ)...
October 31, 2017: Experimental Parasitology
Florencia C Mansilla, Alejandra V Capozzo
Neospora caninum, an intracellular protozoan parasite from the phylum Apicomplexa, is the etiologic agent of neosporosis, a disease considered as a major cause of reproductive loss in cattle and neuromuscular disease in dogs. Bovine neosporosis has a great economic impact in both meat and dairy industries, related to abortion, premature culling and reduced milk yields. Although many efforts have been made to restrain bovine neosporosis, there are still no efficacious control methods. Many vaccine-development studies focused in the apicomplexan proteins involved in the adhesion and invasion of the host cell...
October 31, 2017: Experimental Parasitology
Claudineide Nascimento Fernandes de Oliveira, Tarsila Ferraz Frezza, Vera Lúcia Garcia, Glyn Mara Figueira, Tiago Manuel Fernandes Mendes, Silmara Marques Allegretti
Human schistosomiasis is an important neglected tropical disease caused by blood flukes of the genus Schistosoma and is responsible for more than 280,000 deaths annually. Treatment for this disease relies currently on a single drug, praziquantel (PZQ). Concerns regarding PZQ resistance and insensitivity of juvenile schistosomes have increased the interest in resorting to medicinal plants for alternative drug therapies. This study aimed to perform an in vivo schistosomicidal activity evaluation of crude hexanic (HE) and ethanolic (EE) extracts obtained from Phyllanthus amarus in mice infected with Schistosoma mansoni (BH strain)...
October 23, 2017: Experimental Parasitology
Abdur Rehman, Rizwan Ullah, Neeshma Jaiswal, M A Hannan Khan, Lubna Rehman, Mirza Ahmar Beg, Sandeep K Malhotra, S M A Abidi
The helminth parasites possess great capabilities to adapt themselves within their hosts and also develop strategies to render the commonly used anthelmintics ineffective leading to the development of resistance against these drugs. Besides using anthelmintics the natural products have also been tested for their anti-parasitic effects. Therapeutic efficacy of honey bee venom (HBV) has been tested in various ailments including some protozoal infections but very little is known about its anthelmintic properties...
October 22, 2017: Experimental Parasitology
Hongyu Han, Yan Yan, Hui Dong, Shunhai Zhu, Qiping Zhao, Qi Zhai, Bing Huang
Small heat shock proteins (sHsps) are ubiquitous and diverse molecular chaperones. Found in almost all organisms, they regulate protein refolding and protect cells from stress. Until now, no sHsp has been characterized in Eimeria tenella. In this study, the novel EtsHsp20.4 gene was cloned from E. tenella by rapid amplification of cDNA ends based on a previously identified expressed sequence tag. The full-length cDNA was 1019bp in length and contained an open reading frame of 558bp that encoded a 185-amino acid polypeptide with a calculated molecular weight of 20...
October 18, 2017: Experimental Parasitology
Alvaro De Obeso Fernandez Del Valle, Jacob Lorenzo-Morales, Sutherland K Maciver
Leptomyxa valladaresi was isolated from soil in a pine forest on the southern flank of Mt Teide in Tenerife, Spain. It feeds on bacteria and on a range of other amoebae, and it was possible to establish bi-axenic cultures with L. valladaresi and Acanthamoeba. It is easily propagated on a E. coli also. 18S rDNA gene sequence analysis suggests that it is most closely related to Leptomyxa variabilis, however this amoeba differs in important detail. L. valladaresi is primarily mononucleate whereas L. variabilis is multinucleate...
October 16, 2017: Experimental Parasitology
Naglaa Fathy Abd El-Aal, Rania Said Hamza, Ola Harb
Schistosomiasis is a chronic helminthic disease causing hepatic fibrosis. Some studies demonstrated direct effect of targeting apoptosis on fibrosis regression. This study is a novel trial of Paeoniflorin (PAE) on S. mansoni induced hepatic fibrosis in murine model compared to Praziquantel (PZQ) evaluating their anti-parasitic and anti-fibrotic properties aiming to discover a new therapy that decrease schistosomiasis morbidity. Thirty two laboratory bred Swiss albino male CD-1 mice were used in this study...
October 14, 2017: Experimental Parasitology
Xiaola Guo, Xueyong Zhang, Jing Yang, Xiaoliang Jin, Juntao Ding, Haitao Xiang, Mazhar Ayaz, Xuenong Luo, Yadong Zheng
Echinococcus multilocularis metacestodes are a causative pathogen for alveolar echinococcosis in human beings, and have been found to express miRNAs including emu-miR-71. miR-71 is evolutionarily conserved and highly expressed across platyhelminths, but little is known about its role. Here it was shown that emu-miR-71 was differentially expressed in protoscoleces and was unlikely to be expressed in neoblasts. The results of the luciferase assay indicated that emu-miR-71 was able to bind in vitro to the 3'-UTR of emu-nlk, encoding a key regulator of cell division, causing significant downregulation of luciferase activity (p < 0...
October 13, 2017: Experimental Parasitology
Joyce G R Souza, Juberlan S Garcia, Ana Paula N Gomes, José Roberto Machado-Silva, Arnaldo Maldonado
Echinostoma paraensei (Digenea: Echinostomatidae) lives in the duodenum and bile duct of rodents and is reported as a useful model for studies on the biology of flatworms. Here, we compared the growth and development of pre and post ovigerous worms collected 3, 7, 14 and 21 days post infection from experimentally infected hamster (permissive host) and Wistar rat (less permissive hosts). Linear measurements and ratios were examined by light (morphology and morphometry) and confocal laser scanning microscopy...
October 13, 2017: Experimental Parasitology
Bahram Nikmanesh, Hossein Mirhendi, Shahram Mahmoudi, Mohammad Bagher Rokni
Echinococcus granulosus is now considered a complex consisting of at least four species and ten genotypes. Different molecular targets have been described for molecular characterization of E. granulosus; however, in almost all studies only one or two of the targets have been used, and only limited data is available on the utilization of multiple loci. Therefore, we investigated the genetic diversity among 64 strains isolated from 138 cyst specimens of human and animal isolates, using a set of nuclear and mitochondrial genes; i...
October 9, 2017: Experimental Parasitology
Rebecca Nespoli Lima, Paulo Marcos Zech Coelho, Ana Carolina A Mattos, Clélia Christina Mello Silva, Ronaldo de Carvalho Augusto, Ester Maria Mota, Samaly de Souza, Georgia Correa Atella, Suellen Silva Cabral, George Eduardo Gabriel Kluck, Marta Julia Faro
No abstract text is available yet for this article.
October 7, 2017: Experimental Parasitology
Arturo González-Robles, Maritza Omaña-Molina, Lizbeth Salazar-Villatoro, Catalina Flores-Maldonado, Jacob Lorenzo-Morales, María Reyes-Batlle, Francisco Arnalich-Montiel, Adolfo Martínez-Palomo
Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites appeared to cross the cell monolayer through the tight junctions, which resulted in decreased trans-epithelial resistance (TER) measurements. Unexpectedly, after co-incubation of amoebae with hamster corneas, we observed that the trophozoites were able to cross the different cell layers and reach the corneal stroma after only 12 h of interaction, in contrast to other Acanthamoeba species...
September 30, 2017: Experimental Parasitology
Mejdi Boukari, Khalil Erraïs, Imen Ammous, Khaoula El Amri-Mezghanni, Imen Zhioua-Braham, Meriam Bouchekoua, Sonia Trabelsi, Dorsaf Aloui, Samira Khaled, Mabrouka Saidani, Sonia Mabrouk, Ilhem Mili-Boussen, Raja Zhioua
No abstract text is available yet for this article.
September 28, 2017: Experimental Parasitology
Farhat Abjani, Naveed Ahmed Khan, Suk Yul Jung, Ruqaiyyah Siddiqui
The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept(®), AO SEPT PLUS, OPTI-FREE(®) pure moist(®), Renu(®) fresh™, FreshKon(®) CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT)...
September 15, 2017: Experimental Parasitology
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