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Experimental Parasitology

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https://www.readbyqxmd.com/read/27915089/complete-mitochondrial-genome-of-parasitic-nematode-cylicocyclus-nassatus-and-comparative-analyses-with-cylicocyclus-insigne
#1
Yuan Gao, Jian-Hua Qiu, Bing-Bing Zhang, Xin Su, Xue Fu, Dong-Mei Yue, Chun-Ren Wang
Cylicocyclus nassatus is a common and important parasite in the large intestine of equine. In this study, the complete mitochondrial (mt) genome sequence of C. nassatus was determined and comparatively analysed with Cylicocyclus insigne. The mt genome size of C. nassatus was 13,846 bp, 18 bp longer than that of C. insigne. The circular mt genome includes 12 protein-coding genes, two rRNA genes, 22 tRNA genes and two non-coding regions. All the genes are transcribed in the same direction and gene arrangement is consistent with that of gene arrangement 3 (GA3)...
November 30, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27913109/protein-profiles-of-taenia-solium-cysts-obtained-from-skeletal-muscles-and-the-central-nervous-system-of-pigs-search-for-tissue-specific-proteins
#2
José Navarrete-Perea, Bárbara Moguel, Raúl José Bobes, Nelly Villalobos, Julio César Carrero, Edda Sciutto, Xavier Soberón, Juan Pedro Laclette
Taeniasis/cysticercosis caused by the tapeworm Taenia solium is a parasite disease transmitted among humans and pigs, the main intermediate host. The larvae/cysts can lodge in several tissues of the pig, i.e. skeletal muscles and different locations of the central nervous system. The molecular mechanisms associated to tissue preferences of the cysts remain poorly understood. The major public health concern about this zoonosis is due to the human infections by the larval form in the central nervous system, causing a highly pleomorphic and debilitating disease known as neurocysticercosis...
November 29, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27913108/chicken-line-dependent-mortality-after-experimental-infection-with-three-type-iixiii-recombinant-toxoplasma-gondii-clones
#3
G Schares, D C Herrmann, P Maksimov, B Matzkeit, F J Conraths, G Moré, R Preisinger, S Weigend
Three genetically different clones of Toxoplasma gondii, also different in mouse virulence, were studied by experimental infection in chickens. For the experiments, four chicken lines were used, which differed in phylogenetic origin and performance level: two white egg layer lines, one with high laying performance (WLA), one with low (R11) and two brown layer lines, also displaying high (BLA) and low (L68) egg number. Chickens were intraperitoneally infected with three different T. gondii isolates representing type IIxIII recombinant clones, i...
November 29, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27884580/a-benzimidazole-derivative-rcb20-in%C3%A2-vitro-induces-an-activation-of-energetic-pathways-on-taenia-crassiceps-orf-strain-cysticerci
#4
Carolina Miguel Fraga, Tatiane Luiza da Costa, Ana Maria de Castro, Olivia Reynoso-Ducoing, Javier Ambrosio, Alicia Hernández-Campos, Rafael Castillo, Marina Clare Vinaud
Human cysticercosis caused by Taenia crassiceps is unusual; however, it is an useful experimental model for cysticercosis studies. Benzimidazole derivatives are important antihelminthic drugs widely used against helminths. A novel compound 6-chloro-5-(1-naphthyloxy) -2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative less polar and more lipophilic. The aim of this study was to detect the effect of the RCB20 on the in vitro energetic metabolism of T. crassiceps cysticerci. For this, products of the metabolism both produced and secreted/excreted (S/E) by the parasite were detected through spectrophotometry and high performance liquid chromatography after exposure to 6...
November 21, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27876473/expression-of-truncated-babesia-microti-apical-membrane-protein-1-and-rhoptry-neck-protein-2-and-evaluation-of-their-protective-efficacy
#5
Guanbo Wang, Artemis Efstratiou, Paul Franck Adjou Moumouni, Mingming Liu, Charoonluk Jirapattharasate, Huanping Guo, Yang Gao, Shinuo Cao, Mo Zhou, Hiroshi Suzuki, Ikuo Igarashi, Xuenan Xuan
In this study, we evaluated the protective effect of recombinant Babesia microti apical membrane protein 1 (rBmAMA1) and rhoptry neck protein 2 (rBmRON2) against B. microti infection using a hamster model. The genes encoding the predicted domains I and II of BmAMA1 and the gene encoding the predicted transmembrane regions 2 and 3 of BmRON2 were expressed as His fusion recombinant proteins in Escherichia coli. Three groups with 5 hamsters in each group were immunized with rBmAMA1, rBmRON2 and rBmAMA1+rBmRON2, then challenged with B...
November 19, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27876472/evaluation-of-recombinant-antigens-in-combination-and-single-formula-for-diagnosis-of-feline-toxoplasmosis
#6
Abdelbaset Eweda Abdelbaset, Hend Alhasan, Doaa Salman, Mohamed Hassan Karram, Mahmoud Abd Ellah Rushdi, Xuan Xuenan, Makoto Igarashi
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T. gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT)...
November 19, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27815127/larvicidal-activity-of-essential-oil-of-peumus-boldus-molina-and-its-ascaridole-enriched-fraction-against-culex-quinquefasciatus
#7
Débora Silva Borges de Castro, Denise Brentan da Silva, Jacqueline Domingues Tibúrcio, Marcos Eduardo Guerra Sobral, Vany Ferraz, Alex Gutterres Taranto, João Máximo de Siqueira, Stênio Nunes Alves
Culex quinquefasciatus (Say, 1823), known as the domestic mosquito, is a common and abundant species throughout the world, and a cosmopolitan species. The adults of this mosquito are important in terms of public and animal health since they display adaptability to different hosts. In humans, they are responsible for the transmission of various diseases. One manner of control of this vector is the use of insecticidal or larvicidal products, which may have the drawback of toxicity to mammals and can be harmful to the environment...
November 1, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27810319/gene-flow-for-echinococcus-granulosus-metapopulations-determined-by-mitochondrial-sequences-a-reliable-approach-for-reflecting-epidemiological-drift-of-parasite-among-neighboring-countries
#8
Mahmoud Mahami-Oskouei, Azam Kaseb-Yazdanparast, Adel Spotin, Abbas Shahbazi, Mohammad Adibpour, Ehsan Ahmadpour, Nader Ghabouli-Mehrabani
In genetic diversity and population structure of Echinococcus granulosus, the gene flow can illustrate how the Echinococcus isolates have epidemiologically drifted among endemic neighboring countries. 51 isolates of hydatid cysts were collected from human, dog, cattle and sheep in northwest Iran, where placed co-border with Turkey. DNA samples were extracted, amplified and subjected to sequence analysis of NADH dehydrogenase subunit 1 (nad1) and cytochrome oxidase subunit 1 (cox1) genes. As well, sequences of Echinococcus at east to the southeast regions of Turkey were retrieved from GenBank database for the cox1 gene...
October 31, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27769720/molecular-analyses-reveal-two-geographic-and-genetic-lineages-for-tapeworms-taenia-solium-and-taenia-saginata-from-ecuador-using-mitochondrial-dna
#9
Danilo Solano, Juan Carlos Navarro, Antonio León-Reyes, Washington Benítez-Ortiz, Richar Rodríguez-Hidalgo
Tapeworms Taenia solium and Taenia saginata are the causative agents of taeniasis/cysticercosis. These are diseases with high medical and veterinary importance due to their impact on public health and rural economy in tropical countries. The re-emergence of T. solium as a result of human migration, the economic burden affecting livestock industry, and the large variability of symptoms in several human cysticercosis, encourage studies on genetic diversity, and the identification of these parasites with molecular phylogenetic tools...
October 18, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27765656/dynamic-changes-in-the-main-regulatory-genes-of-mitochondrial-permeability-transition-pore-in-eimeria-tenella-host-cells
#10
Zhi-Yong Xu, Ming-Xue Zheng, Yan Zhang, Xiao-Zhen Cui, Sha-Sha Yang, Rui-Li Liu, Shan Li, Rou Xi, Xin Gong, Rui Bai
The purpose of the present study was to investigate the dynamic changes in the main regulatory genes of the mitochondrial permeability transition pore in E. tenella host cells. Primary chick embryo cecum epithelial cell culture techniques, spectrophotometer technology, Hoechst-Annexin V-PI apoptosis staining and ELISA were used to detect the apoptosis rate and dynamic changes of Bcl-2, Bcl-xl, Bax, Bak, Bid, Bad, HK-II, and ATP content in E. tenella host cells at 4, 24, 48, 72, 96, and 120 h. The rates of early apoptosis, late apoptosis, and necrosis of group T0 were significantly lower (P < 0...
October 17, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27765655/threading-a-novel-insilico-indagation-method-for-genetic-characterization-of-some-diplostomoid-metacercariae-digenea-diplostomidae-poirier-1886
#11
Saroj Rajvanshi, Kirti Choudhary, Nirupama Agrawal
The protein encoding zone of Mitochondrial DNA region (inherited from single lineage) seems most suitable and effective for taxonomic, systematic, ecological, evolutionary, DNA barcoding, cryptic species and population studies, exploiting nucleotide/amino acid datasets (1D/2D/3D conformational level). Nowadays, expeditious computerized methods are in trend for analyzing genetic material to demonstrate variations at various levels of protein structures. Structural proteomics have implemented here for genetic identification, differentiation and relationship of species from information rich data of mt COI gene of the family Diplostomidae with inclusion of molecular tools...
October 17, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27751770/nitazoxanide-induces-in-vitro-metabolic-acidosis-in-taenia-crassiceps-cysticerci
#12
Eliana Isac, Guaraciara de A Picanço, Tatiane L da Costa, Nayana F de Lima, Daniella de S M M Alves, Carolina M Fraga, Ruy de S Lino Junior, Marina C Vinaud
Nitazoxanide (NTZ) is a broad-spectrum anti-parasitic drug used against a wide variety of protozoans and helminthes. Albendazole, its active metabolite albendazole sulfoxide (ABZSO), is one of the drugs of choice to treat both intestinal and tissue helminth and protozoan infections. However little is known regarding their impact on the metabolism of parasites. The aim of this study was to compare the in vitro effect of NTZ and ABZSO in the glycolysis of Taenia crassiceps cysticerci. The cysticerci were treated with 1...
October 14, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27751769/recombinant-protein-of-haemonchus-contortus-14-3-3-isoform-2-rhcftt-2-decreased-the-production-of-il-4-and-suppressed-the-proliferation-of-goat-pbmcs-in-vitro
#13
Javaid Ali Gadahi, Muhammad Ehsan, Shuai Wang, ZhenChao Zhang, Yujian Wang, RuoFeng Yan, XiaoKai Song, LiXin Xu, XiangRui Li
14-3-3 proteins have been found to be an excreted/secreted antigen and assumed to be released into the host-parasite interface and described in several unicellular and multicellular parasites. However, little is known about the immunomodulatory effects of H. controtus 14-3-3 protein on host cell. In present study, 14-3-3 isoform 2 gene, designated as Hcftt-2, was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from the adult H. contortus cDNA and cloned into expression plasmid pET32a (+) and expression of the recombinant protein (rHcftt-2) was induced by IPTG...
October 14, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27746165/kinetic-and-avidity-of-igy-anti-toxocara-antibodies-in-experimentally-infected-chickens
#14
Ricardo da Silva Raposo, Vamilton Alvares Santarém, Yslla Fernanda Fitz Balo Merigueti, Guita Rubinsky-Elefant, Letícia Maria de Lima Cerazo, Ludimilla Pereira, Bianca Pelegi Zampieri, Aristeu Vieira da Silva, Cecília Braga Laposy
Toxocariasis is a geohelminth zoonosis with worldwide distribution, mainly transmitted through the ingestion of embryonated eggs of nematodes of the Toxocara genus. The disease can also be transmitted to humans as a result of eating raw or undercooked meat of paratenic hosts, such as chickens. Here, we standardized an enzyme-linked immunosorbent assay (ELISA) for evaluating experimentally the kinetic and avidity index (AI) of IgY in broiler chickens infected with different doses of Toxocara canis eggs (G1:100; G2: 1000; and G3: 5000; n = 12 per group)...
October 13, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27743973/the-influence-of-angiostrongylus-cantonensis-nematoda-metastrongylidae-infection-on-the-aerobic-metabolism-of-biomphalaria-straminea-and-biomphalaria-tenagophila-mollusca-gastropoda
#15
Mariana G Lima, Vinícius M Tunholi-Alves, Fabrício N Gaudêncio, Florence G Martins, Rosane N Castro, Silvana C Thiengo, Juberlan S Garcia, Arnaldo Maldonado, Jairo Pinheiro
Angiostrongylus cantonensis is considered the main agent responsible for human eosinophilic meningoencephalitis. This parasite has low specificity for mollusk hosts and it can also use aquatic snails as auxiliary hosts. Studies based on the metabolic profile of Biomphalaria spp. infected by A. cantonensis have been conducted to observe parasite-host interactions. In the present study, the glucose content in the hemolymph and glycogen content in the digestive gland and cephalopedal mass of Biomphalaria tenagophila and Biomphalaria straminea experimentally infected by A...
October 12, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27743972/aminoquinoline-compounds-effect-of-7-chloro-4-quinolinylhydrazone-derivatives-against-leishmania-amazonensis
#16
Luciana Maria Ribeiro Antinarelli, Isabela de Oliveira Souza, Nicolas Glanzmann, Ayla das Chagas Almeida, Gabriane Nascimento Porcino, Eveline Gomes Vasconcelos, Adilson David da Silva, Elaine Soares Coimbra
In this study, we have investigated the antileishmanial activity of ten 7-chloro-4-quinolinylhydrazone derivatives. Among the compounds tested, compounds 2a and 2j presented activity against promastigotes (IC50 values of 52.5 and 21.1 μM, respectively) and compounds 2a and 2c were active against intracellular amastigotes (IC50 of 8.1 and 15.6 μM, respectively) of Leishmania amazonensis. The majority of compounds did not show toxicity against murine macrophages. Compound 2a exhibited low cytotoxicity to human erythrocytes and induced an oxidative imbalance in promastigote forms, reflected by an increase in the formation of reactive oxygen species (ROS) and a reduction of mitochondrial membrane potential...
October 12, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27729251/identification-of-immunogenic-proteins-from-ovarian-tissue-and-recognized-in-larval-extracts-of-rhipicephalus-boophilus-microplus-through-an-immunoproteomic-approach
#17
Patricia Berenice Ramírez Rodríguez, Rodrigo Rosario Cruz, Delia Inés Domínguez García, Rodolfo Hernández Gutiérrez, Rodolfo Esteban Lagunes Quintanilla, Daniel Ortuño Sahagún, Celia González Castillo, Abel Gutiérrez Ortega, Sara Elisa Herrera Rodríguez, Adriana Vallejo Cardona, Moisés Martínez Velázquez
Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the use of chemical acaricides; however, their uncontrolled use has increased tSresistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. The only anti-tick vaccine commercially available at present in the world is based on intestinal Bm86 protein, and shows a variable effectiveness depending on tick strains or geographic isolates...
October 8, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27729250/pentamidine-antagonizes-the-benznidazole-s-effect-in-vitro-and-lacks-of-synergy-in-vivo-implications-about-the-polyamine-transport-as-an-anti-trypanosoma-cruzi-target
#18
Verónica Seguel, Lorena Castro, Chantal Reigada, Leonel Cortes, María V Díaz, Mariana R Miranda, Claudio A Pereira, Michel Lapier, Carolina Campos-Estrada, Antonio Morello, Ulrike Kemmerling, Juan D Maya, Rodrigo López-Muñoz
Benznidazole is the first-line drug used in treating Chagas disease, which is caused by the parasite Trypanosoma cruzi (T. cruzi). However, benznidazole has limited efficacy and several adverse reactions. Pentamidine is an antiprotozoal drug used in the treatment of leishmaniasis and African trypanosomiasis. In T. cruzi, pentamidine blocks the transport of putrescine, a precursor of trypanothione, which constitutes an essential molecule in the resistance of T. cruzi to benznidazole. In the present study, we describe the effect of the combination of benznidazole and pentamidine on isolated parasites, mammalian cells and in mice infected with T...
October 8, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27725159/subtype-analysis-of-blastocystis-isolates-using-ssu-rrna-dna-sequencing-in-rural-and-urban-population-in-southern-turkey
#19
Ismail Soner Koltas, Fadime Eroglu
Blastocystis is a common and emerging parasite often seen in many studies conducted in urban population, with scanty reports on rural communities. However, little is known about the public health significance of Blastocystis infection. A total of 28 Blastocystis isolates from 17 (17/28, 60.71%) patients living in rural area and 11 (11/28, 39.29%) patients living in urban area were screened with seven kinds of sequenced-tagged site primers for identification of subtype. PCR products were sequenced with same combination of primers using the BigDye Terminator V 3...
October 7, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27725158/optimization-of-extraction-method-and-evaluation-of-antileishmanial-activity-of-oil-and-nanoemulsions-of-pterodon-pubescens-benth-fruit-extracts
#20
Éverton da Silva Santos, Francielle Pelegrin Garcia, Priscila Miyuki Outuki, Jaqueline Hoscheid, Paulo Roberto Nunes de Goes, Lúcio Cardozo-Filho, Celso Vataru Nakamura, Mara Lane Carvalho Cardoso
Currently, leishmaniasis is difficult to manage owing to the limited choice and high toxicity of available drugs, and emergence of drug-resistant protozoa. Medicinal plants, which produce various bioactive molecules, can help counter this global shortage. In this study, we prepared Pterodon pubescens fruit extracts, which show antileishmanial activity, and developed a nanoemulsion of the optimized extract to improve its performance. The extracts were prepared using conventional methods and a supercritical fluid method and were tested for activity against Leishmania amazonensis promastigotes and amastigotes...
October 7, 2016: Experimental Parasitology
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