journal
MENU ▼
Read by QxMD icon Read
search

Experimental and Molecular Pathology

journal
https://www.readbyqxmd.com/read/29778884/aqp4-and-hivan
#1
REVIEW
Fahad Mubariz, Joseph L Bryant, Vamshi K C Nimmagadda, Jayanta Ray, Tapas K Makar
No abstract text is available yet for this article.
May 17, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29775572/gene-therapy-with-antisense-oligonucleotides-silencing-c-myc-reduces-neointima-formation-and-vessel-wall-thickness-in-a-mouse-model-of-vein-graft-disease
#2
Christina Maria Steger, Nikolaos Bonaros, Ralf Joachim Rieker, Johannes Bonatti, Thomas Schachner
Gene therapy for avoiding intimal hyperplasia of vein grafts after coronary artery bypass grafting is still discussed controversially. A promising application of gene therapy in vein grafts is the use of antisense oligonucleotides to block the expression of genes encoding cell cycle regulatory proteins in vascular smooth muscle cells. C-myc, either directly or by regulating the expression of other proteins, controls cell proliferation, apoptosis and cell survival, tissue remodeling, angiogenesis, cell metabolism, production of inflammatory and anti-inflammatory cytokines, and also participates in cell transformation...
May 15, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29775571/effect-of-methylglyoxal-on-reactive-oxygen-species-ki-67-and-caspase-3-expression-in-mcf-7-cells
#3
Dyah Paramita, J D P Wisnubroto
BACKGROUND: Methylglyoxal (MG) is an extremely reactive α-ketoaldehyde endogenously produced by various metabolic pathways, including the dephosphorylation of glycolytic intermediates, metabolites of the polyol pathway, and aminoacetone metabolism. MG cytotoxicity occurs through the induction of apoptosis. There are multiple mechanisms by which MG can induce apoptosis, e.g., through the generation of reactive oxygen species (ROS), induction of oxidative DNA damage, or accumulation of a specific MG-derived advanced glycation end product (AGE)...
May 15, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29772453/the-roles-of-cypa-and-cd147-in-cardiac-remodelling
#4
REVIEW
Hongyan Su, Yi Yang
Cyclophilin A (CyPA), an abundantly expressed protein belonging to the immunophilin family, is involved in a variety of physiological and pathological activities together with its extracellular receptor, CD147. Many studies have provided compelling evidence supporting critical roles of CyPA in immune and infectious diseases and malignant tumours. Recent studies have revealed that both CyPA and CD147 strongly promote cardiovascular inflammation, myocardial ischaemia-reperfusion injury, and myocardial remodelling processes...
May 14, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29758186/supplementation-with-l-glutathione-improves-oxidative-status-and-reduces-protein-nitration-in-myenteric-neurons-in-the-jejunum-in-diabetic-rattus-norvegicus
#5
Catchia Hermes-Uliana, Flávia Cristina Vieira Frez, Camila Caviquioli Sehaber, Francielle Veiga Ramalho, Fernando Pinheiro de Souza Neto, Rubens Cecchini, Flávia Alessandra Guarnier, Jacqueline Nelisis Zanoni
Diabetes mellitus is a syndrome with multiple etiologies, characterized by chronic hyperglycemia that increases the production of reactive oxygen species and decreases antioxidant defenses. The present study evaluated oxidative stress parameters and protein nitration in myenteric neurons in the jejunum in diabetic rats supplemented with l-glutathione. Rats (90 days of age) were distributed into four groups (n = 6/group): normoglycemic (N), normoglycemic supplemented with l-glutathione (NGT), diabetic (D), and diabetic supplemented with l-glutathione (DGT)...
May 11, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29727604/hepatoprotective-effect-of-fullerenol-doxorubicin-nanocomposite-in-acute-treatment-of-healthy-rats
#6
Danijela Petrovic, Mariana Seke, Milica Labudovic Borovic, Danica Jovic, Ivana Borisev, Branislava Srdjenovic, Zlatko Rakocevic, Vladimir Pavlovic, Aleksandar Djordjevic
In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress...
May 1, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29715436/detection-of-her2-amplification-in-formalin-fixed-paraffin-embedded-breast-carcinoma-tissue-with-digital-pcr-using-two-tff3-sequences-as-internal-reference
#7
Runliu Yu, Wenjing Xuan, Lei Zhou, Ying Luo, Xinyang Liu, Peiwu Xiong, Xuyi Ren
PURPOSE: Droplet digital PCR (ddPCR) is a highly accurate method to determine DNA concentration and detect copy number variations. We developed an approach to assess HER2 gene amplification status using ddPCR with two sequences of TFF3 as reference probes. EXPERIMENTAL DESIGN: 76 templates of carcinoma DNA were prepared from formalin-fixed paraffin-embedded (FFPE) tissues. Digital PCR assay of the copy number of HER2 and TFF3 DNA was performed on the samples. The results were compared to prior fluorescent in-situ hybridation (FISH) assays performed on the same samples...
April 28, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29702112/alpha-syntrophin-deficient-mice-are-protected-from-adipocyte-hypertrophy-and-ectopic-triglyceride-deposition-in-obesity
#8
Kristina Eisinger, Lisa Rein-Fischboeck, Markus Neumeier, Sandra Schmidhofer, Rebekka Pohl, Elisabeth M Haberl, Gerhard Liebisch, Andrea Kopp, Andreas Schmid, Sabrina Krautbauer, Christa Buechler
Alpha-syntrophin (SNTA) is a molecular adapter protein which is expressed in adipocytes. Knock-down of SNTA in 3T3-L1 preadipocytes increases cell proliferation, and differentiated adipocytes display small lipid droplets. These effects are both characteristics of healthy adipose tissue growth which is associated with metabolic improvements in obesity. To evaluate a role of SNTA in adipose tissue morphology and obesity associated metabolic dysfunction, SNTA deficient mice were fed a standard chow or a high fat diet...
April 24, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29653092/cd10-inhibits-cell-motility-but-expression-is-associated-with-advanced-stage-disease-in-colorectal-cancer
#9
Teresa P Raposo, Mireia Sueca Comes, Adeyemi Idowu, Bora Agit, James Hassall, Wakkas Fadhil, Robert Nica, Rupert Ecker, Takashi Yao, Mohammad Ilyas
INTRODUCTION: CD10 is a cell membrane-bound endopeptidase which is expressed in normal small bowel but not in normal colon. It is aberrantly expressed in a small proportion of colorectal cancers (CRC) and this has been associated with liver metastasis and poor prognosis. We sought to investigate the mechanism of CD10 activity and its association with clinicopathological features. MATERIAL AND METHODS: CD10 was stably knocked down by lentiviral shRNA transduction in the CRC cell lines SW480 and SW620 which are derived from a primary tumour and its corresponding metastasis respectively...
April 11, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29641993/pre-clinical-validation-of-a-next-generation-sequencing-testing-panel
#10
Jessica Lüsebrink, Monika Pieper, Ramona-Liza Tillmann, Michael Brockmann, Oliver Schildgen, Verena Schildgen
OBJECTIVE: Next Generation Sequencing (NGS) has become a useful tool for gene mutation testing which is required for targeted therapies. The aim of this study was to validate the GeneRead QIAact Actionable Insights Tumor Panel (Qiagen) on the GeneReader System in a diagnostic laboratory setting. METHODS: The GeneRead QIAact Actionable Insights Tumor Panel allows the analysis of 773 variant positions in 12 genes (ALK, BRAF, EGFR, ERBB2, ERBB3, ESR1, KIT, KRAS, NRAS, PDGFRA, PIK3CA and RAF1)...
April 8, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29621477/stromal-vascular-fraction-promotes-fibroblast-migration-and-cellular-viability-in-a-hyperglycemic-microenvironment-through-up-regulation-of-wound-healing-cytokines
#11
Shaun S Tan, Xin Yi Yeo, Zhen Chang Liang, Sunil K Sethi, Samuel S W Tay
Diabetic wounds have impaired healing and a propensity for further morbidity, which may result in amputations. Stromal vascular fraction (SVF) is an autologous source of heterogeneous cell population obtained from adipose tissue, which is rich in stem cells and presents little immunogenicity to the host. In this study, we hypothesized that murine fibroblasts subjected to hyperglycemic conditions co-treated with SVF exhibit greater functional activity through the colorimetric MTT assay and a cell-monolayer in-vitro scratch assay...
April 2, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29608913/shallow-whole-genome-sequencing-for-robust-copy-number-profiling-of-formalin-fixed-paraffin-embedded-breast-cancers
#12
Suet-Feung Chin, Angela Santonja, Marta Grzelak, Soomin Ahn, Stephen-John Sammut, Harry Clifford, Oscar M Rueda, Michelle Pugh, Mae A Goldgraben, Helen A Bardwell, Eun Yoon Cho, Elena Provenzano, Federico Rojo, Emilio Alba, Carlos Caldas
Pathology archives with linked clinical data are an invaluable resource for translational research, with the limitation that most cancer samples are formalin-fixed paraffin-embedded (FFPE) tissues. Therefore, FFPE tissues are an important resource for genomic profiling studies but are under-utilised due to the low amount and quality of extracted nucleic acids. We profiled the copy number landscape of 356 breast cancer patients using DNA extracted FFPE tissues by shallow whole genome sequencing. We generated a total of 491 sequencing libraries from 2 kits and obtained data from 98...
March 30, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29608912/glomerular-mitochondrial-changes-in-hiv-associated-renal-injury
#13
Joseph L Bryant, Poornachander R Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K C Nimmagadda, Tapas K Makar
HIV-associated nephropathy (HIVAN) is an AIDs-related disease of the kidney. HIVAN is characterized by severe proteinuria, podocyte hyperplasia, collapse, glomerular, and tubulointerstitial damage. HIV-1 transgenic (Tg26) mouse is the most popular model to study the HIV manifestations that develop similar renal presentations as HIVAN. Viral proteins, including Tat, Nef, and Vpr play a significant role in renal cell damage. It has been shown that mitochondrial changes are involved in several kidney diseases, and therefore, mitochondrial dysfunction may be implicated in the pathology of HIVAN...
March 30, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29608911/renal-aquaporin-4-associated-pathology-in-tg-26-mice
#14
Joseph L Bryant, Poornachander R Guda, Sugata Ray, Girma Asemu, Avinash R Sagi, Fahad Mubariz, Muhammed I Arvas, Omar S Khalid, Vivek Shukla, Vamshi K C Nimmagadda, Tapas K Makar
Human immunodeficiency virus-associated nephropathy (HIVAN) is a leading cause of end-stage renal disease in HIV patients, which is characterized by glomerulosclerosis and renal tubular dysfunction. Aquaporin-4 (AQP-4) is a membrane bound water channel protein that plays a distinct role in water reabsorption from renal tubular fluid. It has been proven that failure of AQP-4 insertion into the renal tubular membrane leads to renal dysfunction. However, the role of AQP-4 in HIVAN is unclear. We hypothesize that impaired water reabsorption leads to renal injury in HIVAN, where AQP-4 plays a crucial role...
March 30, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29551574/unilateral-localized-conjunctival-amyloidosis-in-a-patient-with-a-history-of-contralateral-orbit-eyelid-lymphoma
#15
Joshua T Byers, Xin Qing, Christopher Lo, Samuel W French, Ping Ji
Amyloidosis is a disorder characterized by the deposition of insoluble abnormal proteins in the extracellular space. It may occur as a localized lesion or as a systemic disease involving multiple organs and systems. Localized conjunctival amyloidosis is rare and is less frequently associated with systemic involvement. Although amyloidosis itself is a benign lesion involvement of multiple organs and systems is associated with poor prognosis. Diagnosis of amyloidosis is made on biopsy specimens with Congo red staining for the appearance of apple-green birefringence under polarized light microscopy...
April 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29551573/rural-distribution-of-human-papilloma-virus-in-low-and-middle-income-countries
#16
Aaron Atkinson, Courtney Studwell, Suyapa Bejarano, Ana Marcela Zapata Castellón, Jorge Arturo Plata Espinal, Sophie Deharvengt, Ethan P M LaRochelle, Linda S Kennedy, Gregory J Tsongalis
Cervical cancer rates in low- and middle-income countries (LMICs) are higher than in developed countries and account for 80% of an estimated 500,000 new cases annually. Factors that contribute to this are that diagnostic and prevention strategies designed for developed countries suffer from the combination of low vaccination rates and limitations due to lack of consistent access to both healthcare and supplies. Here we: 1) improve upon our LMIC deployable HPV test and 2) determine both the high and low-risk HPV genotype prevalence in an isolated Honduran population...
April 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29530464/role-of-gene-polymorphisms-haplotypes-and-serum-levels-of-interleukin-17a-in-susceptibility-to-viral-myocarditis
#17
Hui Tang, Hua Pei, Qianfeng Xia, Yimei Tang, Ji Huang, Jie Huang, Fang Pei
Interleukin-17A (IL-17A) has been implicated in the pathogenesis of viral myocarditis (VMC). However, the role of IL-17A polymorphisms in susceptibility to VMC has not been reported to date. The aim of this study was to explore the association between IL-17A variants as well as serum IL-17 levels with VMC. Three single-nucleotide polymorphisms (SNPs) (rs2275913, rs3819025, and rs3748067) were analyzed by the polymerase chain reaction-restriction fragment length polymorphism method in 236 VMC patients and 259 controls from China...
April 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29501750/mixed-phenotype-t-b-myeloid-extramedullary-blast-crisis-as-an-initial-presentation-of-chronic-myelogenous-leukemia
#18
Xin Qing, Annie Qing, Ping Ji, Samuel W French, Holli Mason
BACKGROUND: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder characterized by the Philadelphia (Ph) chromosome generated by the reciprocal translocation t(9,22)(q34;q11). The natural progression of the disease follows a biphasic or triphasic course. Most cases of CML are diagnosed in the chronic phase. Extramedullary blast crisis rarely occurs during the course of CML, and is extremely rare as the initial presentation of CML. CASE PRESENTATION: Here, we report the case of a 32-year-old female with enlarged neck lymph nodes and fatigue...
April 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29475036/adamts-6-is-a-predictor-of-poor-prognosis-in-patients-with-esophageal-squamous-cell-carcinoma
#19
Lan Liu, Zhaoting Yang, Weidong Ni, Yanhua Xuan
BACKGROUND: A disintegrin and metalloprotease with thrombospondin motif (ADAMTS) enzymes play important roles in cell functions including adhesion, invasion, migration, and proliferation. ADAMTS-6 is a member of the ADAMTS family; reports of its relationship with esophageal squamous cell carcinoma (ESCC) progression are rare. It is unclear whether ADAMTS-6 could be an independent ESCC biomarker. METHODS: ADAMTS-6 expression was detected by immunohistochemistry (IHC) in 171 paraffin-embedded ESCC specimens; relationships with patients' clinicopathological features and Twist-1 expression were analyzed by the Pearson Chi-square method, respectively...
April 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29452080/an-unusual-presentation-of-primary-pulmonary-extranodal-marginal-zone-lymphoma-of-mucosal-associated-lymphoid-tissue-an-autopsy-case-report
#20
LETTER
Joshua T Byers, Justin L Kurtz, Alejandro S Mendoza, Cyrus D Oster, Nora C J Sun, Xin Qing, Marcia E Cornford
A 40 year old female with no documented medical history presented to the Emergency Department with several days of lethargy and altered mental status. She was found to be anemic, thrombocytopenic, and hypotensive. The patient was found to be in severe metabolic acidosis, became bradycardic, and quickly deteriorated. Clinicians suspected thrombotic thrombocytopenic purpura, and the diagnosis was supported by ADAMTS13 testing. The clinicians attempted to place a Quinton catheter for emergent plasmapheresis, but the patient expired before definitive treatment could be initiated...
April 2018: Experimental and Molecular Pathology
journal
journal
23387
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"