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Experimental and Molecular Pathology

Zhao-Bin Yang, Wen-Wen Chen, Hui-Ping Chen, Shu-Xian Cai, Jian-Dong Lin, Lu-Zhen Qiu
OBJECTIVE: Liver is uniquely vulnerable during sepsis. MicroRNA-155 (miR-155) is confirmed to play crucial roles in septic liver injury. The present study aims to investigate the mechanisms of miR-155 in septic liver injury. METHODS: The sepsis model was established by intraperitoneal injection of lipopolysaccharide (LPS) in mice. Mice were divided into four groups: Vehicle, miR-155 antagomir, LPS, LPS+ miR-155 antagomir. The survival rate and body weight were monitored...
September 12, 2018: Experimental and Molecular Pathology
Abeer A Bahnassy, Salem E Salem, Mohammad El-Sayed, Ola Khorshid, Mona S Abdellateif, Amira S Youssef, Marwa Mohanad, Marwa Hussein, Abdel-Rahman N Zekri, Nasr M Ali
AIM: To assess the role of aberrant miRNAs expressions in stage II CRC patients from Egypt. METHODS: Tumor tissue samples were obtained from 124 CRC stage II patients compared to 100 healthy controls for assessing miRNAs expression using; 1) a cataloged 84-miRNAs PCR array panel, and 2) another five miRNAs (miR-21, miR-137, miR-145, miR-320 and miR-498) that have been reported in previous studies to have a role in CRC, by quantitative real time PCR (qPCR). The results were correlated to patients' characteristics, response to treatment and survival...
September 10, 2018: Experimental and Molecular Pathology
Crismita Dmello, Sharada Sawant, Pratik Rajeev Chaudhari, Harsh Dongre, Chetan Ahire, Zinia Charlotte D'Souza, Silvania Emlit Charles, Pallavi Rane, Daniela Elena Costea, Davendra Chaukar, Shubhada Kane, Milind Vaidya
OBJECTIVE: We have previously reported the aberrant expression of vimentin in human oral premalignant lesions and a 4-Nitroquinoline 1-oxide (4NQO) model of rat lingual carcinogenesis. Hence, we wanted to understand whether the expression of vimentin in early stage contributes to the process of transformation. STUDY DESIGN: Vimentin was stably expressed in oral premalignant lesion derived cells (vimentin negative) and various transformation related phenotypic assays were performed...
September 3, 2018: Experimental and Molecular Pathology
Dongxu Zhao, Minglei Zhang, Hongping Yuan, Chunyang Meng, Boyin Zhang, Han Wu
The aim of this study was to confirm the effects of ginsenoside Rb1 on neural cell apoptosis in the spinal cord of rats with spinal cord ischemia-reperfusion injury (SCII) and to explore its potential mechanisms. A total of 100 healthy adult Sprague-Dawley (SD) rats were randomly divided into four groups: normal control (n = 10), sham-operated (n = 10), SCII model (n = 40), and ginsenoside Rb1-treated groups (n = 40). Basso, Beattie, Bresnahan (BBB) scale was used to examine rat hindlimb locomotor function...
September 2, 2018: Experimental and Molecular Pathology
Ji Ma, Yong Ding
Gastric cancer (GC) is a serious disease with high incidence rate and high mortality. Geniposide (GEN) exhibits multiple biological properties including anti-tumor function. However, effect of GEN on GC is not well studied. Hence, the effects of GEN on GC were investigated in our study. HULC expression in GC tissue and GC cell lines (MKN45, SGC-7901, MKN28, AGS) was detected by qRT-PCR. Cell viability, colony formation, migration, invasion and cell apoptosis were examined by CCK-8 assay, survival fraction assay, modified two-chamber migration assay, Millicell Hanging Cell Culture and flow cytometry analysis, respectively...
August 31, 2018: Experimental and Molecular Pathology
Hao Sun, Qingsong Li, Yingji Jin, Hongmei Qiao
OBJECTIVE: Recently, potential correlations between tumor necrosis factor-α (TNF-α) polymorphisms and asthma were investigated by some pilot studies, but the results of these studies were inconsistent. Therefore, we performed this meta-analysis to better evaluate the relationship between TNF-α polymorphisms and the risk of asthma. METHODS: Eligible studies were searched in PubMed, Medline, Embase and CNKI. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to assess correlations between TNF-α polymorphisms and asthma...
August 31, 2018: Experimental and Molecular Pathology
Olaia Aurtenetxe, Laura Zaldumbide, Asier Erramuzpe, Ricardo López, José I López, Jesús M Cortés, Rafael Pulido, Caroline E Nunes-Xavier
Regulation of growth and differentiation of neuroblastoma (NB) cells is the rational of some maintenance therapies for high-risk NB. MAP kinase phosphatases (MKPs) are potential physiologic regulators of neuronal differentiation and survival, but their expression patterns in NB are scarcely known. Here, an expression analysis of the MKP family has been performed using human NB tumor samples and human NB cell lines (SH-SY5Y, SMS-KCNR, and IMR-32) undergoing retinoic acid (RA)-induced differentiation or subjected to stimuli that activate the MAPK ERK1/2 pathway...
August 29, 2018: Experimental and Molecular Pathology
Zhaoting Yang, Chengye Zhang, Wenbo Qi, Chunai Cui, Yan Cui, Yanhua Xuan
Although Tenascin-C (TNC) as an extracellular matrix protein involved in various cancers, the mechanisms by which TNC leads to decreased survival time remain to be clarified in CRC. We assessed the expression of TNC and its relationship with cancer associated fibroblasts (CAFs) markers, epithelial-to-mesenchymal transition (EMT) and cell cycle markers in 100 paraffin-embedded CRC tissue samples using immunohistochemistry. TNC expression was higher in CRC tissue samples than in adjacent non-tumor-tissues (P < ...
August 28, 2018: Experimental and Molecular Pathology
Melvin Cohn
A successful immune attack on a tumor requires two elements. First, a nonself (NS) epitope that can act as an effective target must be expressed uniquely and uniformly on the tumor. Second, the immune system must be induced to produce an appropriate ridding effector activity specific for the cell expressing that de novo displayed NS-epitope. Today, the major effort is directed toward nonspecifically increasing the horsepower of the immune system by relieving suppressive and resistance factors using checkpoint blockade...
August 18, 2018: Experimental and Molecular Pathology
Lv Chun-Yan, Zhao Zi-Yi, Yang Tian-Lin, Wang Yi-Li, Li Bao, Lv Jiao, Ding Wei-Jun
Renal interstitial fibrosis (RIF) is difficult to diagnose. This paper explored liquid biopsy markers in urinary exosomes derived from RIF patients. Urine samples from 32 patients with various degrees of RIF and 20 non-RIF patients were collected. The size and morphology of urinary exosomes isolated by polyethylene glycol were observed with electron microscopy. Protein biomakers of exosomes were analyzed by Western blot. qRT-PCR was used to detect the levels of biomarkers (miR-29c, miR-21, E-cadherin, and vimentin) of epithelial mesenchymal transition in urinary exosomes...
August 16, 2018: Experimental and Molecular Pathology
Alexander N Orekhov, Tatiana Pushkarsky, Yumiko Oishi, Nikita G Nikiforov, Andrey V Zhelankin, Larisa Dubrovsky, Vsevolod J Makeev, Kathy Foxx, Xueting Jin, Howard S Kruth, Igor A Sobenin, Vasily N Sukhorukov, Emile R Zakiev, Anatol Kontush, Wilfried Le Goff, Michael Bukrinsky
High density lipoproteins (HDL) are key components of reverse cholesterol transport pathway. HDL removes excessive cholesterol from peripheral cells, including macrophages, providing protection from cholesterol accumulation and conversion into foam cells, which is a key event in pathogenesis of atherosclerosis. The mechanism of cellular cholesterol efflux stimulation by HDL involves interaction with the ABCA1 lipid transporter and ensuing transfer of cholesterol to HDL particles. In this study, we looked for additional proteins contributing to HDL-dependent cholesterol efflux...
August 16, 2018: Experimental and Molecular Pathology
Xingyuan Jia, Limei Yao, Yan Zhou, Han Han, Ning Tang, Lirong Wang, Ying Li
Small oligonucleotides mutations are the large majority causes of β-thalassemia. Dual priming oligonucleotide PCR has been used to detect point mutations and thus could be applied to diagnose β-thalassemia. The goal of this study was to establish a simple, quick and cost-effective screening assay by using modified dual priming oligonucleotide PCR for three most common mutations of β-thalassemia [CD71-72 (+A), CD 41-42 (-CTTT), Pnt.-28 (A → G)] in Southeast Asia and southern China. Man-made 5 tandem mismatched bases instead of poly (I) were used as the linker in the specific PCR primers...
August 15, 2018: Experimental and Molecular Pathology
Dmiry V Chistyakov, Alina A Astakhova, Marina G Sergeeva
Relationship between mood disorders and inflammation is now well-documented, although molecular mechanisms are not understood. Previously mostly pro-inflammatory cytokines of immune system (IL-6, TNF, etc.) were taken into account. However, recent understanding of resolution of inflammation as an active process drew attention to mediators of resolution, which include both proteins and ω-3 and ω-6 polyunsaturated fatty acids derivatives (resolvins, cyclopentenone prostaglandins, etc.). This review takes into account new data on resolution of inflammation and action of mediators of resolution in models of depression...
August 8, 2018: Experimental and Molecular Pathology
Tijana Išić Denčić, Aleksandar Bartolome, Sonja Šelemetjev, Ilona Đorić, Svetislav Tatić, Vladan Živaljević, Dubravka Cvejić
We have evaluated the clinical significance of deregulated expression of β-catenin and epidermal growth factor receptor (EGFR) during papillary thyroid carcinoma (PTC) progression. Immunohistochemical expression of β-catenin and EGFR was analyzed in 104 archival tissues of PTC and 19 matched lymph node metastases (LNMs). β-catenin (39/104, 37.5%) and EGFR (58/104, 55.7%) were co-expressed and co-localized in primary PTCs (p < .0001), which was confirmed by double immunofluorescent staining. The high expression of each molecule, as well as their high cytosolic co-expression, correlated with adverse clinicopathological features of the patients (p < ...
August 2, 2018: Experimental and Molecular Pathology
Kinga Szurián, Irén Csala, Dóra Marosvári, Hajnalka Rajnai, Katalin Dezső, Csaba Bödör, Violetta Piurkó, András Matolcsy, Lilla Reiniger
Lymph node involvement of chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL) is characterised by the diffuse infiltration of small neoplastic lymphocytes, which is accompanied by the presence of proliferation centres (PCs) comprising prolymphocytes and paraimmunoblasts. There is increasing evidence of accumulation of various molecular alterations in the tumour cells of PCs, which may explain why extended PCs are related to a less favourable prognosis. To further characterize PCs, we compared the expression level of EZH2 protein, the overexpression of which has recently been recognized as poor prognostic factor in CLL/SLL, in the PCs and the intervening small cell areas in lymph nodes of 15 patients with CLL/SLL...
July 18, 2018: Experimental and Molecular Pathology
Dimitry A Chistiakov, Vladimir P Chekhonin
In the late stages of their development, cancers can form metastases. Formation of metastases was found to be associated with the capacity of cancer cells to quit the tumor mass and journey through the circulation to distant organs. This cell population is called circulating tumor cells (CTCs). They exhibit several advanced properties such as epithelial-to-mesenchymal transition (EMT) and dormancy that are essential for supporting their survival in the bloodstream, radio- and chemoresistance, their escape from the anti-cancer immune response, and metastasis initiation...
July 18, 2018: Experimental and Molecular Pathology
Jonathan Ciriello, Alexandru Tatomir, Daniel Hewes, Dallas Boodhoo, Freidrich Anselmo, Violeta Rus, Horea Rus
We have previously shown that SIRT1 mRNA expression was significantly lower in relapsing MS patients compared to those in remission. Our goal was to longitudinally investigate the role of active, phosphorylated SIRT1 (p-SIRT1) as a potential biomarker of relapse and predictor for response to glatiramer acetate (GA) treatment in patients with relapsing remitting multiple sclerosis (MS). We also want to investigate the downstream effects of SIRT1 activation by measuring the trimethylation of histone 3 at lysine 9 (H3K9me3)...
July 17, 2018: Experimental and Molecular Pathology
Iván Martín, Blanca Navarro, Eva Villamón, Carlos Solano, Alicia Serrano, Marisa Calabuig, Paula Amat, Fernando Domingo, Rosario Abellán, Francisca García, María Dolores Olivares, Francisco Javier Chaves, Mar Tormo, Juan Carlos Hernández-Boluda
BACKGROUND: Therapy-related acute myeloid leukemia (t-AML) develops in patients with prior exposure to cytotoxic therapies. Selection of a pre-existing TP53 mutated clone prone to acquire additional mutational events has been suggested as the main pathogenic mechanism of t-AML. Here, we report a unique case of t-AML which developed from a pre-existing DNMT3A mutated clone that persisted in the patient for more than 10 years despite treatment with intensive chemotherapy and allogeneic hematopoietic stem cell transplantation (alloHSCT)...
August 2018: Experimental and Molecular Pathology
Samuel W French, Jiajie G Lu
The nature of the immunologic synapse in autoimmune hepatitis is defined. This process involves the T cell receptor (TCR) which binds to the hepatocyte antigen presenting major histocompatibility complex (MHC) on the plasma membrane. This complex is quickly removed from the liver cell and taken into the T cell cytoplasm to be digested by the lysosome. The liver cell is gradually diminished to the point of its total removal by the lymphocytes binding to it.
August 2018: Experimental and Molecular Pathology
Xiangyan Zhang, Lili Wang, Jigang Wang, Han Zhao, Jie Wu, Shuhong Liu, Lu Zhang, Yujun Li, Xiaoming Xing
AIMS: To investigate whether immunohistochemistry (IHC) could be used to screen BRAF mutation status in formalin-fixed paraffin-embedded (FFPE) colorectal carcinoma (CRC) and papillary thyroid carcinoma (PTC) samples. METHODS: Eight surgical resected samples, including 2 CRCs with mutated BRAF V600E, 2 PTCs with mutated BRAF V600E, 2 CRCs with wild-type BRAF and 2 PTCs with wild-type BRAF, were selected to explore the optimized IHC conditions for BRAF V600E (VE1) immunostaining using BenchmarkXT automated immunostainer VENTANA Medical System...
August 2018: Experimental and Molecular Pathology
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