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Experimental Eye Research

Wilhelm Lindenau, Heidrun Kuhrt, Elke Ulbricht, Katrin Körner, Andreas Bringmann, Andreas Reichenbach
Mammalian retinal glial (Müller) cells are known to guide light through the inner retina to photoreceptors (Franze et al., 2007; Proc Natl Acad Sci U S A 104:8287-8292). It was shown that Müller cells transmit predominantly red-green and less violet-blue light (Labin et al., 2014; Nat Commun 5:4319). It is not known whether this optical function is reflected in the cone-to-Müller cell ratio. To determine this ratio in the retinas of mammals with different lifestyle, we evaluated the local densities of cones and Müller cells in the retinas of guinea pigs, rabbits, sheep, red deer, roe deer, domestic pigs, and wild boars...
January 11, 2019: Experimental Eye Research
Chen Liang, LanYa Peng, ShaoXue Zeng, Qing Zhao, Linqiao Tang, XiaoShuang Jiang, JunJun Zhang, Naihong Yan, YingYing Chen
The purpose of this study was to investigate indoleamine 2,3-dioxygenase 1 (IDO1) expression and its implications in uveal melanoma (UM). Bioinformatics analysis was performed on microarray data (GSE22138 and GSE27831) from the Gene Expression Omnibus (GEO) database to evaluate IDO1 expression in mRNA level. Ninety-two cases in the database were divided into the IDO1-high group (46 cases) and IDO1-low group (46 cases). Paraffin embedded tumor sections from 27 patients with UM were studied by immunofluorescence...
January 10, 2019: Experimental Eye Research
Keiko Usui-Kusumoto, Hiroki Iwanishi, Kana Ichikawa, Yuka Okada, Takayoshi Sumioka, Masayasu Miyajima, Chia-Yang Liu, Peter S Reinach, Shizuya Saika
Corneal neovascularization and inflammatory fibrosis induced by severe injury or infection leads to tissue opacification and even blindness. Transient receptor potential (TRP) channel subtypes contribute to mediating these maladaptive responses through their interactions with other receptors. TRPV1 is one of the contributing channel isoforms inducing neovascularization in an alkali burn mouse wound healing model. VEGF-A upregulation contributes to neovascularization through interaction with its cognate receptors (VEGFR)...
January 8, 2019: Experimental Eye Research
Diana Cristina Pérez-Ibave, María Lourdes Garza-Rodríguez, Antonio Alí Pérez-Maya, Irám Pablo Rodríguez-Sánchez, Maricela Luna-Muñoz, Carlos Guillermo Martínez-Moreno, Carlos Arámburo-de la Hoz, Jibran Mohamed-Noriega, Karim Mohamed-Noriega, Jesús Mohamed-Hamsho, Víctor Manuel Bautista-De Lucío, Hugo Alberto Barrera-Saldaña
In humans, the polygenic growth hormone (GH) locus is located on chromosome 17 and contributes with three types of proteins: pituitary GH which consists of at least two isoforms one of 22 kDa and the other of 20 kDa, placental GH, which also exhibits isoforms, and chorionic somatomammotropin hormone (CSH). While pituitary GH results from the expression of the GH-1 (GH-N) gene, placental GH is produced by the expression of the GH-2 (GH-V) gene, and CSH is contributed by expression of the CSH-1, and CSH-2 genes...
January 8, 2019: Experimental Eye Research
Yiqin Bao, Feng Liu, Xiaobin Liu, Mi Huang, Liumin He, Seeram Ramakrishna, Huanmin Luo, Huiling Hu, Hongying Li, Ying Xu
Methyl 3,4 dihydroxybenzoate (MDHB) is a small molecule that shows neuroprotective effects in vitro and in a photoreceptor-degenerative mouse model. Here we investigated whether MDHB protects retina in a mouse model of acute ocular hypertension (AOH) and explores the underlying mechanisms. AOH was induced in mice by increasing intraocular pressure to approximately 90 mmHg for 60 min, then MDHB or vehicle was intraperitoneally injected daily up to 7 days. Immunostaining and multi-electrode array recordings were performed to examine the structure and function of retinas receiving the treatments...
January 8, 2019: Experimental Eye Research
Michelle E LeBlanc, Weiwen Wang, Yanli Ji, Hong Tian, Dachuan Liu, Xuxiang Zhang, Wei Li
Wet age-related macular degeneration (AMD) with choroidal neovascularization (CNV) is a leading cause of vision loss in the elderly. The advent of anti-vascular endothelial growth factor (VEGF) drugs represents a major breakthrough in wet AMD therapy but with limited efficacy to improve visual acuity. Secretogranin III (Scg3, SgIII) was recently discovered as a novel angiogenic factor with VEGF-independent mechanisms. Scg3-neutralizing monoclonal antibody (mAb) was reported to alleviate pathological retinal neovascularization in oxygen-induced retinopathy mice and retinal vascular leakage in diabetic mice with high efficacy and disease selectivity...
January 8, 2019: Experimental Eye Research
Debora L Nickla, Kelsey Jordan, Jane Yang, Puneet Singh
Injections of the D2 dopamine receptor agonist quinpirole or the acetylcholine muscarinic receptor antagonists pirenzepine and atropine prevent the development of negative-lens-induced myopia in chicks by inhibiting ocular growth. Because ocular growth is diurnally rhythmic, we hypothesized that the efficacy for inhibition may depend on time of day. Chicks wore monocular -10D lenses for 5 days, starting at 12d of age. The light cycle was 12L/12D. The lens-wearing eye received daily intravitreal injections for 4 days, of 20 μl quinpirole (10 nmol), at the following times: 7:30 EST (lights-on; morning; n = 12), 12:00 (mid-day; n = 13), or 19:30 (evening; n = 17)...
January 7, 2019: Experimental Eye Research
Xiangyang Xin, Yong Zhang, Feng Ling, Liming Wang, Xiaohong Sheng, Liru Qin, Xin Zhao
The present study aims to construct a miRNA-based predictive signature of Uveal melanoma (UM) based on the database of the cancer genome atlas (TCGA). We obtained miRNA expression profiles and clinical information of 80 UM patients from TCGA, and randomly divided them into a training and a testing set. After data processing and forward screening, a total of 204 miRNAs with prognostic value were then examined by the Cox proportional hazard regression model in the training set. Receiver operating curve (ROC) analysis was applied to validate the accuracy of the signature...
January 4, 2019: Experimental Eye Research
Johanna M Walz, Thomas Wecker, Pei Pei Zhang, Bertan Cakir, Bjoern Gruening, Hansjuergen Agostini, Tristan Reuer, Franziska Ludwig, Stefaniya Boneva, Lothar Faerber, Clemens Lange, Guenther R Schlunck, Andreas Stahl
BACKGROUND: Human retinal microvascular endothelial cells (HRMVECs) are involved in the pathogenesis of retinopathy of prematurity. In this study, the microRNA (miRNA) expression profiles of HRMVECs were investigated under resting conditions, angiogenic stimulation (VEGF treatment) and anti-VEGF treatment. MATERIALS AND METHODS: The miRNA profiles of HRMVECs under resting and angiogenic conditions (VEGF treatment), as well as after addition of aflibercept, bevacizumab or ranibizumab were evaluated by analyzing the transcriptome of small non-coding RNAs...
January 4, 2019: Experimental Eye Research
Claudia Núñez-Álvarez, Neville N Osborne
The aim of the present study was to analyse how short wave blue and long wave red light differentially affect corneal epithelial (HCE-2) cells in culture. The corneal epithelium in situ is exposed to more blue light than in the past because of Light Emitting Diodes (LEDs) used for indoor lighting and computer, television and phone screens. Compared with cultures maintained in the dark, low intensity blue light, such as that emitted from computer screens, reduced the proliferation rate of HCE-2 cells and caused cell death at greater intensities in a dose-dependent manner...
January 3, 2019: Experimental Eye Research
Rajiv R Mohan, Ratnakar Tripathi, Ajay Sharma, Prashant R Sinha, Elizabeth A Giuliano, Nathan P Hesemann, Shyam S Chaurasia
Decorin (Dcn), a small leucine-rich proteoglycan, is involved in the regulation of corneal wound healing. Epidermal growth factor receptor (EGFR) plays a critical role in corneal fibroblasts proliferation, migration and extracellular matrix (ECM) modulation upon injury or infection. The present study aimed to investigate the mechanistic role of Dcn in EGFR internalization to the regulation of corneal stromal fibroblasts (CSFs) migration, a key step in the corneal wound healing. Human corneal stromal fibroblasts (hCSF) cultures were generated from donor corneas...
January 3, 2019: Experimental Eye Research
Ranjay Chakraborty, Victoria Yang, Han Na Park, Erica G Landis, Susov Dhakal, Michael A Bergen, P Michael Iuvone, Machelle T Pardue
Retinal photoreceptors are important in visual signaling for normal eye growth in animals. We used Gnat2cplf3/cplf3 (Gnat2-/- ) mice, a genetic mouse model of cone dysfunction to investigate the influence of cone signaling in ocular refractive development and myopia susceptibility in mice. Refractive development under normal visual conditions was measured for Gnat2-/- and age-matched Gnat2+/+ mice, every 2 weeks from 4 to 14 weeks of age. Weekly measurements were performed on a separate cohort of mice that underwent monocular form-deprivation (FD) in the right eye from 4 weeks of age using head-mounted diffusers...
December 31, 2018: Experimental Eye Research
Guiqiu Zhao
No abstract text is available yet for this article.
December 31, 2018: Experimental Eye Research
Pedro Lax, Oksana Kutsyr, Gema Esquiva, Cesare Altavilla, Victoria Maneu, Nicolás Cuenca
Ocular pathologies and blindness have been linked to circadian disorders. In previous studies, our group has demonstrated that retinitis pigmentosa is associated with degenerative changes in the melanopsin system and weaker circadian patterns. We have also shown that cannabinoids preserve retinal structure and function in dystrophic P23H rats. This study is consequently aimed at examining whether the morphologic and functional rescue of retinal degeneration by cannabinoids is associated with amelioration of circadian parameters...
December 31, 2018: Experimental Eye Research
Claudio Colafella
No abstract text is available yet for this article.
December 28, 2018: Experimental Eye Research
Xi Wei, Dan Luo, Yu Yan, Haiyang Yu, Liyao Sun, Chao Wang, Fanqian Song, Hongyan Ge, Hua Qian, Xiaoguang Li, Xianling Tang, Ping Liu
Fuchs endothelial dystrophy (FED) and late cornea allograft failure of cornea transplantation are associated with human corneal endothelial cells (HCEC) senescence. Kojic acid has various functions, however, its anti-senescence effect has never been identified. In this study, we investigated the anti-senescence effect of kojic acid on HCEC. Cell viability, migration ability and senescence were evaluated by MTT assay, migration assay, and senescence-associated beta-galactosidase (SA-β-Gal) staining, respectively...
December 28, 2018: Experimental Eye Research
Mustapha El Hamdaoui, Drew W Gann, Thomas T Norton, Rafael Grytz
We describe an analysis strategy to obtain ultrasonography-matched axial dimensions of small animal eyes using the LenStar biometer. The LenStar optical low-coherence reflectometer is an attractive device for animal research due to its high precision, non-invasiveness, and the ability to measure the axial dimensions of cornea, anterior chamber, lens, vitreous chamber, and axial length. However, this optical biometer was designed for clinical applications in human eyes and its internal analysis provides inaccurate values when used on small eyes due to species-dependent differences in refractive indices and relative axial dimensions...
December 26, 2018: Experimental Eye Research
Xue Chen, Chao Jiang, Daidi Yang, Ruxu Sun, Min Wang, Hong Sun, Min Xu, Luyin Zhou, Mingkang Chen, Ping Xie, Biao Yan, Qinghuai Liu, Chen Zhao
Retinitis pigmentosa (RP), the most common form of inherited retinal dystrophies, exhibits significant genetic heterogeneity. The crumbs homolog 2 (CRB2) protein, together with CRB1 and CRB3, belongs to the Crumbs family. Given that CRB1 mutations account for 4% of RP cases, the role of CRB2 mutations in RP etiology has long been hypothesized but never confirmed. Herein, we report the identification of CRB2 as a novel RP causative gene in a Chinese consanguineous family and have analyzed its pathogenic effects...
December 26, 2018: Experimental Eye Research
Alexandra Pietraszkiewicz, Christopher Hampton, Sonny Caplash, Ling Lei, Yassemi Cepetanaki, Gauri Tadvalkar, Sonali Pal-Ghosh, Mary Ann Setepp, Paola Bargagna-Mohan, Royce Mohan
The type III intermediate filament (IF) proteins vimentin and desmin are sequentially overexpressed in stromal myofibroblasts over the period when fibrosis sets in after corneal injury. Prior findings have revealed vimentin-deficient mice are significantly protected from corneal fibrosis after alkali injury, which has implicated this IF protein as an important regulator of corneal fibrosis. It has remained as yet unproven whether desmin contributes in any significant manner to corneal fibrosis. Here we have employed desmin-deficient (Des KO) mice in the corneal alkali injury model and show that injured Des KO mice develop fibrosis and show similar levels of corneal opacity at 14 days post-injury as wild type (WT) mice and retain this phenotype even at 30d post injury...
December 24, 2018: Experimental Eye Research
Dianlei Guo, Mengke Li, Bin Zou, Xinyu Gu, Zhaohui Yuan, Shixin Guo, Fuxiang Mao, Hong Ouyang, Kaili Wu, Lai Wei, Yizhi Liu, Chunqiao Liu
Ocular surface disease is one major type of eye diseases. Different etiologies trigger distinct pathological responses of the ocular surface. We previously reported that genetically engineered mice with ablation of Prickle 1 manifested precocious eyelid opening with ensuing cornea dysplasia. The current study aimed to characterize the molecular traits and the direct cause of ocular pathology associated with precocious eyelid opening in the Prickle 1 mutant mouse. Prickle 1 mutant mice exhibited a slew of ocular surface pathology including cell proliferation, cell fate transformation and inflammatory infiltration coinciding with the timing of the precocious eyelid opening...
December 24, 2018: Experimental Eye Research
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