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Experimental Eye Research

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https://www.readbyqxmd.com/read/29339089/concentration-gradient-of-noradrenaline-from-the-periphery-to-the-centre-of-the-cornea-a-clue-to-its-origin
#1
Luis Figueira, Carla Ferreira, Catarina Janeiro, Paula Serrao, Fernando Falcao-Reis, Daniel Moura
We set out to demonstrate that the major source of corneal catecholamines is its neuronal release from intrinsic sympathetic nerves rather than circulating or non-neuronal local production. Three concentric segments (central, intermediate, peripheral) were obtained by double trephination (9.5-7.25 mm) performed on corneas harvested from 3-4 month old rabbits and human corneas rejected for transplantation, along with aqueous humour, full iris tissue and blood samples. Endogenous catecholamines were quantified by high pressure liquid chromatography with electrochemical detection (HPLC-ED), and comparison with the uptake of radio-labelled noradrenaline (3H-NA) before and after incubation with cocaine was performed...
January 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29339088/retinal-neuronal-cell-loss-prevents-abnormal-retinal-vascular-growth-in-a-rat-model-of-retinopathy-of-prematurity
#2
Ayuki Nakano, Daiki Asano, Ryo Kondo, Asami Mori, Shiho Arima, Hiroko Ushikubo, Kenji Sakamoto, Tohru Nagamitsu, Kunio Ishii, Tsutomu Nakahara
A short-term blockade of the vascular endothelial growth factor (VEGF)-mediated pathway in neonatal rats results in formation of severe retinopathy of prematurity (ROP)-like retinal blood vessels. The present study aimed to examine the role of retinal neurons in the formation of abnormal retinal blood vessels. Newborn rats were treated subcutaneously with the VEGF receptor tyrosine kinase inhibitor, KRN633 (10 mg/kg), or its vehicle (0.5% methylcellulose in water) on postnatal day (P) 7 and P8. To induce excitotoxic loss of retinal neurons, N-methyl-D-aspartic acid (NMDA) was injected into the vitreous chamber of the eye on P9...
January 12, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29337142/express-a-database-of-transcriptome-profiles-encompassing-known-and-novel-transcripts-across-multiple-development-stages-in-eye-tissues
#3
Gungor Budak, Soma Dash, Rajneesh Srivastava, Salil A Lachke, Sarath Chandra Janga
Advances in sequencing have facilitated nucleotide-resolution genome-wide transcriptomic profiles across multiple mouse eye tissues. However, these RNA sequencing (RNA-seq) based eye developmental transcriptomes are not organized for easy public access, making any further analysis challenging. Here, we present a new database "Express" (http://www.iupui.edu/∼sysbio/express/) that unifies various mouse lens and retina RNA-seq data and provides user-friendly visualization of the transcriptome to facilitate gene discovery in the eye...
January 11, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29329973/altered-gene-expression-in-tree-shrew-retina-and-retinal-pigment-epithelium-produced-by-short-periods-of-minus-lens-wear
#4
Li He, Michael R Frost, John T Siegwart, Thomas T Norton
Hyperopic refractive error is detected by retinal neurons, which generate GO signals through a direct emmetropization signaling cascade: retinal pigment epithelium (RPE) into choroid and then into sclera, thereby increasing axial elongation. To examine signaling early in this cascade, we measured gene expression in the retina and RPE after short exposure to hyperopia produced by minus-lens wear. Gene expression in each tissue was compared with gene expression in combined retina + RPE. Starting 24 days after normal eye opening, three groups of juvenile tree shrews (n = 7 each) wore a monocular -5 D lens...
January 9, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29326066/novel-everolimus-loaded-nanocarriers-for-topical-treatment-of-murine-experimental-autoimmune-uveoretinitis-eau
#5
Maren Kasper, Doris Gabriel, Michael Möller, Dirk Bauer, Lena Wildschütz, Herve Courthion, Michael R R Böhm, Martin Busch, Karin Loser, Solon Thanos, Robert Gurny, Arnd Heiligenhaus
In the present study, therapeutic effect of topically applied everolimus (EV)-loaded methoxy-poly(ethylene-glycol)-hexyl substituted poly (lactic acid) (mPEGhexPLA) nanocarriers on experimental autoimmune uveitis (EAU) were investigated. EAU was induced in B10.RIII mice via immunization with human interphotoreceptor retinoid-binding protein peptide 161-180 (hIRBPp161-180) in complete Freund's adjuvant. Everolimus-loaded mPEGhexPLA (EV/mPEGhexPLA) nanocarriers were prepared by using a solvent evaporation method...
January 8, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29326065/hydrogen-sulfide-supplement-attenuates-the-apoptosis-of-retinal-ganglion-cells-in-experimental-glaucoma
#6
Shouyue Huang, Ping Huang, Zhongjing Lin, Xiaohong Liu, Xing Xu, Lei Guo, Xi Shen, Changwei Li, Yisheng Zhong
Glaucoma is a group of neurodegenerative eye diseases characterized by progressive impairment of visual function due to loss of retinal ganglion cells (RGC). As hydrogen sulfide (H2S) was reported to play a role in the process of glaucomatous neuropathy and improve RGC survival in experimental glaucoma, the authors aimed to investigate the anti-apoptosis effect of H2S supplement in a rat glaucoma model, and further tried to explore the involved factors in the neuroprotection. A chronic ocular hypertension (COH) rat model induced by intracameral injection of cross-linking hydrogel was employed to simulate glaucoma and 288 rats were subjected to experimental procedures in the present study...
January 8, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29317238/gender-differences-in-mrna-expression-of-aquaporin-8-and-glutathione-peroxidase-in-cataractous-lens-following-intake-of-an-antioxidant-supplement
#7
Rijo Hayashi, Shimmin Hayashi, Miki Sakai, Kiyomi Arai, Makoto Chikuda, Shigeki Machida
Antioxidants can decrease oxidative damage and prevent age-related ocular disease. Our previous investigation on human aqueous humor following intake of a lutein-containing antioxidant supplement reported an increase in the scavenging activity of superoxide in both genders and an increase in the amount of hydrogen peroxide (H2O2) in females. Aquaporin 8 (AQP8) is a diffusion facilitator of H2O2 and glutathione peroxidase (Gpx) is a H2O2 scavenging enzyme. The correlation between AQP8 and Gpx may be the key to determining how oxidative stress in the aqueous humor affects the lens after intake of antioxidant supplements...
January 6, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29305888/comparative-immunological-study-of-penetrating-and-anterior-lamellar-keratoplasty-in-a-murine-model
#8
Hyun Soo Lee, Ji Young Kwon, Choun-Ki Joo
In lamellar keratoplasty, the diseased part of a cornea is replaced while the healthy tissue remains. lamellar keratoplasty has the advantage of better graft survival compared to penetrating keratoplasty (PK). We compared the immunological responses to PK and anterior lamellar keratoplasty (ALK) in a murine model. PK or ALK was performed using C57BL/6 donor grafts and BALB/c recipients, and graft opacity was assessed to evaluate graft rejection up to 8 weeks. We evaluated the immunological responses in both groups, which were not clinically considered as graft failure on postoperative day 21...
January 3, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29305299/identification-of-oaf-and-pvrl1-as-candidate-genes-for-an-ocular-anomaly-characterized-by-keratolenticular-dysgenesis-and-ectopia-lentis
#9
Dezső David, Deepti Anand, Carlos Araújo, Brian Gloss, Joana Fino, Marcel Dinger, Päivi Lindahl, Minna Pöyhönen, Laivuori Hannele, João Lavinha
Keratolenticular dysgenesis (KLD) and ectopia lentis are congenital eye defects. The aim of this study is the identification of molecular genetic alterations responsible for those ocular anomalies with neurologic impairment in an individual with a de novo balanced chromosome translocation t(11;18)(q23.3;q11.2)dn. Disruption of OAF, the human orthologue of the Drosophila oaf, by the 11q23.3 breakpoint results in reduced expression of this transcriptional regulator. Furthermore, four most likely nonfunctional chimeric transcripts comprising up to OAF exon 3, derived from the der(11) allele, have also been identified...
January 2, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29289676/in-response-to-the-analysis-of-human-conjunctival-epithelium-proteome-in-ocular-surface-diseases-using-impression-cytology-and-2d-dige
#10
LETTER
Louis Tong
No abstract text is available yet for this article.
December 28, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29288646/bacteria-induce-autophagy-in-a-human-ocular-surface-cell-line
#11
Kimberly M Brothers, Regis P Kowalski, Shenghe Tian, Paul R Kinchington, Robert M Q Shanks
Autophagy protects cells from intracellular pathogens, but can be exploited by some infectious agents to their benefit. Currently it is not known if bacteria induce autohpagy in cells of the cornea. The goal of this study was to develop an ocular surface autophagy reporter cell line and determine whether ocular bacterial pathogens influence host responses through autophagy induction. The cell line was made using lentivirus transduction of an LC3-GFP fusion protein in human corneal limbal epithelial (HCLE) cells...
December 27, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29288023/anterior-lens-capsule-strains-during-simulated-accommodation-in-porcine-eyes
#12
Antonio Pellegrino, Harvey John Burd, Laura Pinilla Cortés, Justin Christopher D'Antin, Nik Petrinic, Rafael I Barraquer, Ralph Michael
Experimental protocols have been developed to measure the spatial variation of the mechanical strains induced in the lens capsule during ex vivo lens stretching. The paper describes the application of these protocols to porcine lenses. The deformations and mechanical strains developed in the anterior capsule during each experiment were determined using full field digital image correlation techniques, by means of a speckle pattern applied to the lens surface. Several speckling techniques and illumination methods were assessed before a suitable combination was found...
December 26, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29284110/efficacy-of-lenvatinib-a-multitargeted-tyrosine-kinase-inhibitor-on-laser-induced-cnv-mouse-model-of-neovascular-amd
#13
Xian Wei, Ting Zhang, Yuqin Yao, Shaoxue Zeng, Min Li, Haotian Xiang, Chengjian Zhao, Guiqun Cao, Minhui Li, Ran Wan, Ping Yang, Jinliang Yang
Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In this study, the effects of an orally administered multi-targeted tyrosine kinase inhibitor (Lenvatinib, E7080) were evaluated in vitro and in vivo on neovascular AMD mouse model. The results showed that E7080 effectively inhibited the proliferation, migration and tubule formation of human choroidal microvascular endothelial cells (HCMECs), and suppressed the angiogenesis of zebrafish subintestinal vessels without causing malformation...
December 25, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29278698/gwas-links-variants-in-neuronal-development-and-actin-remodeling-related-loci-with-pseudoexfoliation-syndrome-without-glaucoma
#14
Katarzyna Zagajewska, Magdalena Piątkowska, Krzysztof Goryca, Aneta Bałabas, Anna Kluska, Agnieszka Paziewska, Ewelina Pośpiech, Iwona Grabska-Liberek, Ewa E Hennig
Pseudoexfoliation syndrome (PEXS) is an age-related elastosis, strongly associated with the development of secondary glaucoma. It is clearly suggested that PEXS has a genetic component, but this has not been extensively studied. Here, a genome-wide association study (GWAS) using a DNA-pooling approach was conducted to explore the potential association of genetic variants with PEXS in a Polish population, including 103 PEXS patients without glaucoma and 106 perfectly (age- and gender-) matched controls. Individual sample TaqMan genotyping was used to validate GWAS-selected single-nucleotide polymorphism (SNP) associations...
December 23, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29258748/omega-3-fatty-acids-protect-retinal-neurons-in-the-dba-2j-hereditary-glaucoma-mouse-model
#15
Maria Kalogerou, Panagiotis Kolovos, Ekatherine Prokopiou, Gregory Papagregoriou, Constantinos Deltas, Stavros Malas, Tassos Georgiou
The purpose of this study was to evaluate the neuroprotective effects of omega-3 polyunsaturated fatty acid (ω3-PUFA) supplementation, alone or in combination with timolol eye drops, in a mouse model of hereditary glaucoma. DBA/2J mice (8.5-month-old) were assigned to an ω3-PUFAs + timolol, ω3-PUFAs only, timolol only, or an untreated group. Treated mice received a daily gavage administration of eicosapentaenoic acid (EPA) and docosahexaenoic acid and/or topical instillation of timolol (0.5%) once a day for 3 months...
December 16, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29246498/epithelium-derived-mir-204-inhibits-corneal-neovascularization
#16
Xiaoping Zhang, Guohu Di, Muchen Dong, Mingli Qu, Xiaowen Zhao, Haoyun Duan, Xiaoli Hu, Ting Liu, Qingjun Zhou, Weiyun Shi
MicroRNA-204 (miR-204) is highly expressed in cornea, here we explored the role and mechanism of miR-204 in corneal neovascularization (CNV). Mouse CNV was induced by intrastromal placement of suture in BALB/c mice with the subconjunctival injection of miR-204 agomir or negative control. Human primary limbal epithelial cells (LECs) and immortalized microvascular endothelial cells (HMECs) were used to evaluate the expression changes and anti-angiogenic effects of miR-204 under biomechanical stress (BS). The expression and localization of miR-204, vascular endothelial growth factor (VEGF) and their receptors were detected by quantitative real-time PCR, in situ hybridization, immunohistochemistry and Western blot...
December 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29246497/improved-in-vitro-corneal-delivery-of-a-thrombospondin-1-derived-peptide-using-a-liposomal-formulation
#17
Laura Soriano-Romaní, Jesús Álvarez-Trabado, Antonio López-García, Irene Molina-Martínez, Rocío Herrero-Vanrell, Yolanda Diebold
Peptide delivery to and through ocular sites is a growing field of research interest. However, several barriers restrict the permeation and bioavailability of these molecules to target tissues. The main pharmacological barriers of topical administration are the tear film, rapid drainage of the tear film, and poor corneal permeation. If the administered molecule is a peptide, instability and enzymatic degradation can be significant. Novel approaches such as the design and development of nanocarriers to overcome these drawbacks have been investigated with promising results...
December 12, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29242028/blockade-of-kca3-1-a-novel-target-to-treat-tgf-%C3%AE-1-induced-conjunctival-fibrosis
#18
Govindaraj Anumanthan, Philip J Wilson, Ratnakar Tripathi, Nathan P Hesemann, Rajiv R Mohan
Postoperative conjunctival fibrosis is common in patients after glaucoma filtration surgery. The calcium activated potassium (KCa3.1) channel has been shown to inhibit fibrosis in many non-ocular tissues. However, its potential in treating ocular fibrosis remains unknown. We tested the anti-fibrotic potential of TRAM34, a selective blocker of KCa3.1 channel, in treating conjunctival fibrosis. Primary human conjunctival fibroblast (HCF) cultures derived from donor tissues. Myofibroblasts causing conjunctival fibrosis were generated by growing HCFs in the presence of TGFβ1 for 72 h...
December 11, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29242027/m%C3%A3-ller-glial-cells-of-the-primate-foveola-an-electron-microscopical-study
#19
Steffen Syrbe, Heidrun Kuhrt, Ulrich Gärtner, Gunnar Habermann, Peter Wiedemann, Andreas Bringmann, Andreas Reichenbach
Previous studies on the ultrastructure of the primate foveola suggested the presence of an inverted cone-like structure which is formed by 25-35 specialized Müller cells overlying the area of high photoreceptor density. We investigated the ultrastructure of the Müller cells in the foveola of a human and macaque retina. Sections through the posterior poles of an eye of a 40 years-old human donor and an eye of an adult cynomolgus monkey (Macaca fascicularis) were investigated with transmission electron microscopy...
December 11, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/29196060/mir-365-promotes-diabetic-retinopathy-through-inhibiting-timp3-and-increasing-oxidative-stress
#20
Juan Wang, Jieping Zhang, Xin Chen, Yiting Yang, Fang Wang, Weiye Li, Maihemuti Awuti, Yaping Sun, Chunpin Lian, Zongyi Li, Min Wang, Jing-Ying Xu, Caixia Jin, Haibin Tian, Furong Gao, Jingfa Zhang, Debasish Sinha, Lixia Lu, Guo-Tong Xu
miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-365 expression pattern, in situ hybridization and quantitative RT-PCR were performed. Bioinformatical analysis and dual luciferase report assay were applied to identify and confirm target genes...
November 28, 2017: Experimental Eye Research
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