Mu-Rong Kao, Jake Parker, Daniel Oehme, Shu-Chieh Chang, Lin-Chen Cheng, Damao Wang, Vaibhav Srivastava, John M Wagner, Philip J Harris, Yves S Y Hsieh
Barley (1,3;1,4)-β-d-glucanase is believed to have evolved from an ancestral monocotyledon (1,3)-β-d-glucanase, enabling the hydrolysis of (1,3;1,4)-β-d-glucans in the cell walls of leaves and germinating grains. In the present study, we investigated the substrate specificities of variants of the barley enzymes (1,3;1,4)-β-d-glucan endohydrolase [(1,3;1,4)-β-d-glucanase] isoenzyme EII ( Hv EII) and (1,3)-β-d-glucan endohydrolase [(1,3)-β-d-glucanase] isoenzyme GII ( Hv GII) obtained by protein segment hybridization and site-directed mutagenesis...
April 10, 2024: Biochemistry