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Analytical Chemistry

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https://www.readbyqxmd.com/read/29148723/dna-microviscosity-characterization-with-particle-diffusometry-for-downstream-dna-detection-applications
#1
Katherine Noel Clayton, Gregory D Berglund, Jacqueline C Linnes, Tamara L Kinzer-Ursem, Steven T Wereley
Analytical characterization of DNA microviscosity provides critical biophysical insights into nuclear crowding, nucleic acid-based pharmaceutical development, and nucleic acid-based biosensor device design. However, most viscosity characterization methods require large sample volumes and destructive testing. In contrast, particle diffusometry, permits in situ analysis of DNA microviscosity with short measurement times (8 seconds) using small volumes (< 3L) which are compatible with DNA preparatory procedures...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148722/acoustic-wave-driven-functionalized-particles-for-aptamer-based-target-biomolecule-separation
#2
Raheel Ahmad, Ghulam Destgeer, Muhammad Afzal, Jinsoo Park, Husnain Ahmed, Jin Ho Jung, Kwangseok Park, Tae-Sung Yoon, Hyung Jin Sung
We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device comprised of an interdigitated transducer (IDT) that produced high-frequency surface acoustic waves (SAW) in a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid flow inside the microchannel and deflected particles from their original streamlines to achieve separation...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148721/ratiometric-fluorescence-sensing-and-real-time-detection-of-wa-ter-in-organic-solvents-with-one-pot-synthesis-of-ru-mil-101-al-nh2
#3
Hua-Qing Yin, Jichun Yang, Xue-Bo Yin
Ratiometric fluorescence detection attracts much attention because of its decreased environment influence and easy-to-differentiate color and intensity change. Herein, a guest-encapsulation metal organic framework (MOF), Ru@MIL-NH2, is prepared with 2-aminoterephthalic acid, AlCl3, and Ru(bpy)32+ by simple one-pot method for ratiometric fluorescence sensing of water in organic solvents. The rational selection of excitation wavelength provides dual emission at 465 and 615 nm from Ru@MIL-NH2 under single excitation of 300 nm...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148720/a-dual-signal-readout-nanosphere-for-rapid-point-of-care-detection-of-ebola-virus-glycoprotein
#4
Jiao Hu, Yong-Zhong Jiang, Ling-Ling Wu, Zhen Wu, Yuhai Bi, Gary Wong, Xiang-Guo Qiu, Jian-Jun Chen, Dai-Wen Pang, Zhi-Ling Zhang
Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of EBOV glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target as well as to act as the signal enhancer...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148719/effect-of-antigen-retrieval-methods-on-non-specific-binding-of-anti-body-metal-nanoparticle-conjugates-on-ffpe-tissue
#5
Yuying Zhang, Xinping Wang, Sven Perner, Ágnes Bánkfalvi, Sebastian Schlücker
Immunohistochemical analysis of FFPE tissues provides important diagnostic and prognostic information in pathology. Metal nanoparticles (NPs) and in particular surface-enhanced Raman scattering (SERS) nanotags as a new class of labelling reagents are promising to be used for multiplexed protein profiling on tissue sections. However, non-specific binding of NPs onto the tissue specimens greatly hampers their clinical applications. In this study, we found that the antigen retrieval method strongly influences the extent of non-specific binding of the antibody-SERS NP conjugates to the tissue...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148717/raman-spectroscopy-of-single-light-absorbing-carbonaceous-particles-levitated-in-air-using-an-annular-laser-beam
#6
Masaru Uraoka, Keisuke Maegawa, Shoji Ishizaka
A laser trapping technique is a powerful means to investigate the physical and chemical properties of single aerosol particles in a non-contact manner. However, optical trapping of strongly light-absorbing particles such as black carbon or soot is quite difficult because the repulsive force caused by heat is orders of magnitude larger than the attractive force of radiation pressure. In this study, a laser trapping and Raman microspectroscopy system using an annular laser beam was constructed to achieve non-contact levitation of single light-absorbing particles in air...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148716/rapid-quantification-of-4-4-methylenedianiline-by-surface-enhanced-raman-spectroscopy
#7
Samuel L Kleinman, Mark C Peterman, Merwan Benhabib, Michael T Cheng, James D Hudson, Rachel E Mohler
Methylenedianiline (MDA) is a common industrial chemical with health and product safety concerns. Common analysis methods require many steps including extraction and derivatization ending in GC-MS or HPLC analysis, which minimize its use as an on-line or at-line technique. The procedure can take hours, prohibiting its use as a real-time decision-making tool as well as using valuable resources and laboratory space. The new method presented here has been validated for MDA quantification in industrial grease samples over the concentration range of 1-40 ppm 4,4'-MDA...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148714/evaluating-the-rate-and-substrate-specificity-of-laboratory-evolved-xna-polymerases
#8
Ali Nikoomanzar, Matthew R Dunn, John C Chaput
Engineered polymerases that can copy genetic information between DNA and xeno-nucleic acids (XNA) hold tremendous value as reagents in future bio-technology applications. However, current XNA poly-merases function with inferior activity relative to their natural counterparts, indicating that current polymerase engineering efforts would benefit from new benchmark-ing assays. Here, we describe a highly parallel, low cost method for measuring the average rate and substrate specificity of XNA polymerases in a standard qPCR instrument...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148710/lipidqc-method-validation-tool-for-visual-comparison-to-srm-1950-using-nist-interlaboratory-comparison-exercise-lipid-consensus-mean-estimate-values
#9
Candice Zena Ulmer, Jared M Ragland, Jeremy P Koelmel, Alan Heckert, Christina M Jones, Timothy Garrett, Richard A Yost, John A Bowden
As advances in analytical separation techniques, mass spectrometry instrumentation, and data processing platforms continue to spur growth in the lipidomics field, more structurally unique lipid species are detected and annotated. The lipidomics community is in need of benchmark reference values to assess the validity of various lipidomics workflows in providing accurate quantitative measurements across the diverse lipidome. LipidQC addresses the harmonization challenge in lipid quantitation by providing a semi-automated process, independent of analytical platform, for visual comparison of experimental results of National Institute of Standards and Technology Standard Reference Material (SRM) 1950 Metabolites in Frozen Human Plasma against benchmark consensus mean concentrations derived from the NIST Lipidomics Interlaboratory Comparison Exercise...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29125282/smart-chiral-sensing-platform-with-alterable-enantioselectivity
#10
Yin Yu, Yongxin Tao, Baozhu Yang, Datong Wu, Yong Qin, Yong Kong
A quinine (QN)-based chiral sensing platform with alterable enantioselectivity is constructed for electrochemical chiral recognition of tryptophan (Trp) isomers. The electrochemical signals of l- and d-Trp on the QN modified electrode depend closely on temperature, and more particularly are reversed at certain temperatures, which could be attributed to the temperature-sensitive H-bonds and π-π interactions between QN and the Trp isomers. The mechanisms of the reverse chiral recognition are investigated by density functional theory (DFT), variable-temperature UV spectra, and variable-temperature (1)H NMR spectra...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29120603/a-high-sensitivity-integrated-absorbance-and-fluorescence-detection-scheme-for-probing-picoliter-volume-droplets-in-segmented-flows
#11
Tianjin Yang, Stavros Stavrakis, Andrew deMello
Droplet-based microfluidic systems that incorporate flowing streams of pL-volume droplets surrounded by a continuous and immiscible carrier phase have attracted significant recent attention due to their utility in complex chemical and biological experimentation. Analysis of pL droplets, generated at kHz frequencies and moving at high linear velocities, is almost exclusively achieved using fluorescence-based detection schemes. To extend the applicability of such optical detection schemes, we herein report the development of a simple and cost-effective optofluidic platform, integrating liquid-core PDMS waveguides, that allows the accurate measurement of absorbance within individual pL-volume droplets moving within segmented flows...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29120162/y-shaped-dna-duplex-structure-triggered-gold-nanoparticle-dimers-for-ultrasensitive-colorimetric-detection-of-nucleic-acid-with-the-dark-field-microscope
#12
Jingjing Li, Qingyun Liu, Hongyan Xi, Xiangcong Wei, Zhengbo Chen
Herein, we present a novel gold nanoparticle (AuNP) enumeration-based colorimetric aptamer biosensor for ultrasensitive detection of nucleic acid. This AuNP enumeration-based colorimetric method takes advantages of the distinctive and strong localized surface plasmon resonance light scattering with the dark-field microscope. In our model system, first, cost-effective DNA1 instead of expensive 2-thioethyl ether acetic acid was capped on the surface of AuNPs to form a dense DNA1 layer. Then, two DNA strands (DNA2 and DNA3) in two different solutions were separately asymmetrically functionalized on the AuNPs capped dense DNA1 layer...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29115822/single-quantum-dot-based-nanosensor-for-sensitive-detection-of-o-glcnac-transferase-activity
#13
Juan Hu, Yueying Li, Ying Li, Bo Tang, Chun-Yang Zhang
Protein glycosylation is a ubiquitous post-translational modification that plays crucial roles in modulating biological recognition events in development and physiology. Human O-GlcNAc transferase (OGT) is an intracellular enzyme responsible for O-linked N-acetylglucosamine (O-GlcNAc) glycosylation, and the deregulation of OGT activity occurs in cancer, diabetes, and neurodegenerative disease. Here we develop a single quantum dot (QD)-based nanosensor for sensitive OGT assay. We design a Cy5/biotin-modified peptide with a serine hydroxyl group for sensing OGT and a protease site adjacent to the glycosylation site for proteinase cleavage, with a universal nonradioactive UDP-GlcNAc as the sugar donor and a Cy5/biotin-modified peptide as the substrate...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29115126/aggregation-induced-emission-based-chemodosimeter-approach-for-selective-sensing-and-imaging-of-hg-ii-and-methylmercury-species
#14
Amrita Chatterjee, Mainak Banerjee, Dipratn G Khandare, Ram U Gawas, Starlaine C Mascarenhas, Anasuya Ganguly, Rishabh Gupta, Hrishikesh Joshi
Methylmercury (CH3Hg(+)) is the common form of organic mercury and is more toxic than its inorganic or elemental forms. Mercury is emanated in the course of various natural events and human activities and converts to methylmercury by anaerobic organisms. CH3Hg(+) are ingested by fish and subsequently bioaccumulated in their tissue and, eventually, enter the human diet, causing serious health issues. Therefore, selective and sensitive detection of bioaccumulated CH3Hg(+) in fish samples is essential. Herein, the development of a simple, highly sensitive and selective aggregation-induced emission (AIE)-based turn-on probe for both inorganic mercury ions and organicmercury species is reported...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29111686/centrifugation-controlled-thermal-convection-and-its-application-to-rapid-microfluidic-polymerase-chain-reaction-devices
#15
Masato Saito, Kazuya Takahashi, Yuichiro Kiriyama, Wilfred Villariza Espulgar, Hiroshi Aso, Tadanobu Sekiya, Yoshikazu Tanaka, Tsuneo Sawazumi, Satoshi Furui, Eiichi Tamiya
Here, we report the developed cyclo olefin polymer (COP) microfluidic chip on a fabricated rotating heater stage that utilizes centrifugation-assisted thermal cycle in a ring-structured microchannel for polymerase chain reaction (PCR). The PCR solution could be driven by thermal convection and continuously exchanged high/low temperatures in a ring structured microchannel without the use of typical syringe pump. More importantly, the flow rate was controlled by the relative gravitational acceleration only. The platform enables amplification within 10 min at 5G and has a detection limit of 70...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29111679/recombinant-protein-polymers-for-colloidal-stabilization-and-improvement-of-cellular-uptake-of-diamond-nanosensors
#16
Tingting Zheng, Felipe Perona Martínez, Ingeborg Maria Storm, Wolf Rombouts, Joris Sprakel, Romana Schirhagl, Renko de Vries
Fluorescent nanodiamonds are gaining increasing attention as fluorescent labels in biology in view of the fact that they are essentially nontoxic, do not bleach, and can be used as nanoscale sensors for various physical and chemical properties. To fully realize the nanosensing potential of nanodiamonds in biological applications, two problems need to be addressed: their limited colloidal stability, especially in the presence of salts, and their limited ability to be taken up by cells. We show that the physical adsorption of a suitably designed recombinant polypeptide can address both the colloidal stability problem and the problem of the limited uptake of nanodiamonds by cells in a very straightforward way, while preserving both their spectroscopic properties and their excellent biocompatibility...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29111668/ultrasensitive-high-resolution-mass-spectrometric-analysis-of-a-dna-adduct-of-the-carcinogen-benzo-a-pyrene-in-human-lung
#17
Peter W Villalta, J Bradley Hochalter, Stephen S Hecht
Benzo[a]pyrene (BaP), an archetypical polycyclic aromatic hydrocarbon, is classified as "carcinogenic to humans" and is ubiquitous in the environment, as evident by the measurable levels of BaP metabolites in virtually all human urine samples examined. BaP carcinogenicity is believed to occur mainly through its covalent modification of DNA, resulting in the formation of BPDE-N(2)-dG, an adduct formed between deoxyguanosine and a diol epoxide metabolite of BaP, with subsequent mutation of critical growth control genes...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29048159/using-printing-orientation-for-tuning-fluidic-behavior-in-microfluidic-chips-made-by-fused-deposition-modeling-3d-printing
#18
Feng Li, Niall P Macdonald, Rosanne M Guijt, Michael C Breadmore
Fluidic behavior in microfluidic devices is dictated by low Reynolds numbers, complicating mixing. Here, the effect of the orientation of the extruded filament on the fluidic behavior is investigated in fused deposition modeling (FDM) printed fluidic devices. Devices were printed with filament orientations at 0°, 30°, 60°, and 90° to the direction of the flow. The extent of mixing was observed when pumping yellow and blue solutions into the inlets of a Y-shaped device, and measuring the extent of mixing of two colored solutions under different angles and at flow rates of 25, 50, and 100 μL/min...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29144732/ratiometric-detection-of-intracellular-lysine-and-ph-with-one-pot-synthesized-dual-emissive-carbon-dots
#19
Wei Song, Wenxiu Duan, Yinghua Liu, Zhongju Ye, Yonglei Chen, Hongli Chen, Shengda Qi, Jiang Wu, Dan Liu, Lehui Xiao, Cuiling Ren, Xingguo Chen
Recently, the development of new fluorescent probes for the ratiometric detection of target objects inside living cells has received great attention. Normally, the preparation, modification as well as conjugation procedures of these probes are complicated. On this basis, great efforts have been paid to establish convenient method for the preparation of dual emissive nanosensor. In this work, a functional dual emissive carbon dots (dCDs) was prepared by a one-pot hydrothermal carbonization method. The dCDs exhibits two distinctive fluorescence emission peaks at 440 and 624 nm with the excitation at 380 nm...
November 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29144729/an-optical-nanoantenna-for-single-molecule-based-detection-of-zika-virus-nucleic-acids-without-molecular-multiplication
#20
Sarah Elisabeth Ochmann, Carolin Vietz, Kateryna Trofymchuk, Guillermo P Acuna, Birka Lalkens, Philip Tinnefeld
Due to the limited signal-to-background ratio, molecular diagnostics requires molecular amplification of the target molecules or molecular signal amplification after target recognition. For direct molecular detection, we demonstrate a purely physical fluorescence enhancement process which can elevate the fluorescence signal of single fluorescent dyes by several orders of magnitude. To this end, DNA origami-based optical antennas with a height of around 125 nm are used, which utilize metallic nanoparticles to create a hotspot where fluorescence signals are enhanced by plasmonic effects...
November 16, 2017: Analytical Chemistry
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