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Analytical Chemistry

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https://www.readbyqxmd.com/read/30232889/branch-migration-based-fluorescent-probe-for-highly-sensitive-detection-of-mercury
#1
Shanshan Wang, Bin Lin, Li Chen, Na Li, Jiaju Xu, Jing Wang, Yuxiang Yang, Yan Qi, Yongxin She, Xiantao Shen, Xianjin Xiao
Detection of heavy metals is of great importance for food safety and environmental analysis. Among various heavy metal ions, mercury ion is one of the most prevalent species. The methods for detection of mercury were numerous and T-Hg-T based assay was promising due to its simplicity and compatibility. However, traditional T-Hg-T based methods mainly relied on multiple T-Hg-T to produce enough conformational changes for further detection, which greatly restrained the limit of detection. Hence, we established a branch-migration based fluorescent probe and found that single T-Hg-T could produce strong signals...
September 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30232881/terbium-iii-organic-gels-novel-antenna-effect-induced-enhanced-electrochemiluminescence-emitters
#2
Yang Li, Zhong Wei Jiang, Si Yu Xiao, Cheng Zhi Huang, Yuan Fang Li
Metal organic gels (MOGs), emerged as a new class of smart soft materials with superb luminescence properties, have at-tracted tremendous attention in various aspects. However, the electrochemiluminescence (ECL) behavior of MOGs has not been reported yet. In this work, cathode electrochemiluminescence (ECL) emission of terbium (III) organic gels (TOGs) was reported for the first time with potassium persulfate (K2S2O8) as efficient coreactant. TOGs was synthesized by a facile one-step strategy, mixing terbium ions (Tb3+) and the ligand 4'-(4-carboxyphenyl)-2, 2':6', 2''-terpyridine (Hcptpy) at room tem-perature...
September 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30232879/cross-species-molecular-imaging-of-bile-salts-and-lipids-in-liver-identification-of-molecular-structural-markers-in-health-and-disease
#3
Bryn Flinders, Lennart Huizing, Marjolein Van Heerden, Filip Cuyckens, Ulf Neumann, Luc van der Laan, Steven W M Olde Damink, Ron M A Heeren, Frank G Schaap, Rob J Vreeken
The liver is the primary organ involved in handling of bile salts, a class of amphipathic molecules with signaling activities as well as desired and detrimental detergent actions. To allow in-depth investigation of functions of bile salts in healthy and diseased liver, the spatial distribution of bile salt species within the liver needs to be studied. Therefore the aim of our study was to determine hepatic bile salt distribution and identify specific lipid markers that define the structural elements of the liver...
September 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30231202/surface-plasmon-resonance-sensing-on-naturally-derived-membranes-a-remyelination-promoting-human-antibody-binds-myelin-with-extraordinary-affinity
#4
Milan Vala, Luke R Jordan, Arthur E Warrington, L James Maher Iii, Moses Rodriguez, Nathan J Wittenberg, Sang-Hyun Oh
rHIgM22 is a recombinant human monoclonal IgM designed to promote remyelination, and it is currently in Phase I clinical trials in patients with multiple sclerosis (MS). In animal models of demyelination, a single low dose of rHIgM22 stimulates oligodendrocyte maturation, induces remyelination, preserves axons, and slows the decline of locomotor deficits. Natural autoantibodies like rHIgM22 typically bind to multiple antigens with weak affinity. rHIgM22 binds to oligodendrocytes and myelin. Because the antigen(s) for rHIgM22 is prevalent within and exclusive to central nervous system (CNS) myelin, we used CNS myelin particles in combination with surface plasmon resonance to determine the kinetic and affinity constants for the interaction of rHIgM22 to myelin...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30230817/surface-enhanced-raman-scattering-sers-based-microfluidics-for-single-cell-analysis
#5
Marjorie R Willner, Kay McMillan, Duncan Graham, Peter J Vikesland, Michele Zagnoni
The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA) functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of "fast" coarse maps over an area of several mm2 followed by "slower" detailed interrogation of the identified hotspots...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30230816/automated-plasma-glycomics-with-linkage-specific-sialic-acid-esterification-and-ultrahigh-resolution-ms
#6
Gerda C M Vreeker, Simone Nicolardi, Marco R Bladergroen, Corné J van der Plas, Wilma E Mesker, Rob A E M Tollenaar, Yuri E M van der Burgt, Manfred Wuhrer
High throughput mass spectrometry (MS) glycomics is an emerging field driven by technological advancements including sample preparation and data processing. Previously, we reported an automated protocol for the analysis of N-glycans released from plasma proteins that included sialic acid derivatization with linkage-specificity, namely ethylation of α2,6-linked sialic acid residues and lactone formation of α2,3-linked sialic acids. In the current study, each step in this protocol was further optimized. Method improvements included minimizing the extent of side-reaction during derivatization, an adjusted glycan purification strategy and mass analysis of the released N-glycans by ultrahigh resolution matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance MS...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30229657/gold-nanoparticle-couples-with-entropy-driven-toehold-mediated-dna-strand-displacement-reaction-on-magnetic-beads-toward-ultrasensitive-energy-transfer-based-photoelectrochemical-detection-of-mirna-141-in-real-blood-sample
#7
Nan Zhang, Xiao-Mei Shi, Hong-Qian Guo, Xiaozhi Zhao, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Highly stable circulating microRNAs (miRNAs) are currently recognized as a novel potential biomarker for clinical cancer diagnosis in the early stage. However, limited by its low concentration, high sequence similarity as well as the numerous interferences in body fluids, detection of miRNA in whole blood with sufficient selectivity and sensitivity is still challenging. Herein, we reported the integration of entropy-driven toehold-mediated DNA strand displacement (ETSD) reaction with magnetic beads (MB) toward the energy-transfer-based photoelectrochemical (PEC) detection of the prostate carcinoma (PCa) biomarker miRNA-141 in real blood sample...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30227710/co-sensitization-strategy-with-cascade-energy-level-arrangement-for-ultrasensitive-photoelectrochemical-protein-detection
#8
Yan-Hui Zhang, Ying-Ning Zheng, Meng-Jie Li, Tao Hu, Ruo Yuan, Sha-Ping Wei
Here, a photoelectrochemical (PEC) biosensor was established by a co-sensitization strategy with cascade energy level arrangement for ultrasensitive detection of prostate-specific antigen (PSA). The proposed co-sensitization strategy was based on the well-matched energy level arrangement of four kinds of organic photoactive materials, in which poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2 -b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)-carbonyl]thieno[3,4-b]thiophene-4,6-diyl} (PTB7-Th) was used as the photoactive material and perylenetetracarboxyl diimide (PDI), fullerene (nano-C60), polyaniline (PANI) were employed as the sensitizers...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30203652/specific-gene-capture-combined-with-restriction-fragment-release-for-directly-fluorescent-genotyping-of-single-nucleotide-polymorphisms-in-diagnosing-spinal-muscular-atrophy
#9
Chun-Chi Wang, Chung-An Chen, Yuh-Jyh Jong, Hwang-Shang Kou
In this study, a fast and simple fluorescent genotyping strategy, streptavidin magnetic beads combined with biotin-coupled PCR and restriction-fragment release, was developed for determination of nucleotide variants. This method was further applied for analyzing SMN1 gene in diagnosis of spinal muscular atrophy (SMA). After biotin-coupled PCR, the streptavidin magnetic beads would capture the biotin-labeled SMN genetic fragments, and then the restriction enzyme of HPY188I could only digest and release the fluorescent end of SMN1 genetic fragment into the supernatant...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30199243/flexible-continuous-particle-beam-switching-via-external-field-reconfigurable-asymmetric-induced-charge-electroosmosis
#10
Haizhen Sun, Yukun Ren, Weiyu Liu, Xiangsong Feng, Likai Hou, Ye Tao, Hongyuan Jiang
Continuous sample switching is an essential process for developing an integrated platform incorporating multiple functionality with applications typically ranging from chemical to biological assays. Herein we propose a unique method of external-field-reconfigurable symmetry breaking in induced-charge electroosmosis above a simple planar bipolar electrode for continuous particle beam switching. In the proposed system, the spatial symmetry of a nonlinear electroosmotic vortex flow can be artificially reordered to achieve an asymmetric electrically floating-electrode polarization by regulating the configurations of the external ac signals, thus contributing to flexible particle beam switching...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30192525/chemical-derivatization-strategy-for-extending-the-identification-of-mhc-class-i-immunopeptides
#11
Rui Chen, Francois Fauteux, Simon Foote, Jacek Stupak, Tammy-Lynn Tremblay, Komal Gurnani, Kelly M Fulton, Risini D Weeratna, Susan M Twine, Jianjun Li
Neoantigen-based therapeutic vaccines have a high potential impact on tumor eradication and patient survival. Mass spectrometry (MS)-based immunopeptidomics has the capacity to identify tumor-associated epitopes and pinpoint mutation-bearing major histocompatibility complex (MHC)-binding peptides. This approach presents several challenges, including the identification of low-abundance peptides. In addition, MHC peptides have much lower MS/MS identification rates than tryptic peptides due to their shorter sequence and lack of basic amino acid at C-termini...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30192521/high-throughput-separation-trapping-and-manipulation-of-single-cells-and-particles-by-combined-dielectrophoresis-at-a-bipolar-electrode-array
#12
Yupan Wu, Yukun Ren, Ye Tao, Likai Hou, Hongyuan Jiang
Microfluidic systems have been developed widely in scaled-down processes of laboratory techniques, but they are usually limited in achieving stand-alone functionalities. It is highly desirable to exploit an integrated microfluidic device with multiple capabilities such as cell separation, single-cell trapping, and cell manipulation. Herein, we reported a microfluidic platform integrated with actuation electrodes, for separating cells and microbeads, and bipolar electrodes, for trapping, rotating, and propelling single cells and microbeads...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30191711/in-situ-imaging-of-furin-activity-with-a-highly-stable-probe-by-releasing-of-precipitating-fluorochrome
#13
Ke Li, Xiao-Xiao Hu, Hong-Wen Liu, Shuai Xu, Shuang-Yan Huan, Jun-Bin Li, Tang-Gang Deng, Xiao-Bing Zhang
Furin, a kind of trans-Golgi proprotein convertases, plays important role in various physiological processes. It is overexpressed in many cancers and relates to tumor growth and migration. In situ detection and imaging of furin is of great significance for obtaining real-time information about its activity. However, the previously reported fluorescent probes for furin usually failed to realize in situ detection and long-term bioimaging, because these probes are based on water-soluble fluorophores, which tend to diffuse away from the reaction sites after converted by furin...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30189726/efficient-photochemical-vapor-generation-of-molybdenum-for-icpms-detection
#14
Jakub Šoukal, Ralph E Sturgeon, Stanislav Musil
Photochemical vapor generation (PVG) of Mo was accomplished using a 19 W high-efficiency flow-through photoreactor operating in a flow injection mode using 30-50% (w/v) formic acid as a reaction medium. The generated volatile product (most probably molybdenum hexacarbonyl) was directed by an argon carrier gas to a plastic gas-liquid separator and introduced into the spray chamber of an inductively coupled plasma mass spectrometer for detection. Particular attention was paid to the determination of overall PVG efficiency relative to that for liquid nebulization...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30188687/multimodal-chemical-analysis-of-the-brain-by-high-mass-resolution-mass-spectrometry-and-infrared-spectroscopic-imaging
#15
Elizabeth K Neumann, Troy J Comi, Nicolas Spegazzini, Jennifer W Mitchell, Stanislav S Rubakhin, Martha U Gillette, Rohit Bhargava, Jonathan V Sweedler
The brain functions through chemical interactions between many different cell types, including neurons and glia. Acquiring comprehensive information on complex, heterogeneous systems requires multiple analytical tools, each of which have unique chemical specificity and spatial resolution. Multimodal imaging generates complementary chemical information via spatially localized molecular maps, ideally from the same sample, but requires method enhancements that span from data acquisition to interpretation. We devised a protocol for performing matrix-assisted laser desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance-mass spectrometry imaging (MSI), followed by infrared (IR) spectroscopic imaging on the same specimen...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30188686/an-efficient-approach-for-selective-enrichment-of-histone-modification-readers-using-self-assembled-multivalent-photoaffinity-peptide-probes
#16
Guijin Zhai, Hanyang Dong, Zhenchang Guo, Wei Feng, Jin Jin, Tao Zhang, Cong Chen, Pu Chen, Shanshan Tian, Xue Bai, Lei Shi, Enguo Fan, Yukui Zhang, Kai Zhang
Histone post-translational modifications (HPTMs) provide signaling platforms to recruit proteins or protein complexes (e.g., transcription factors, the so-called "readers" of the histone code), changing DNA accessibility in the regulation of gene expression. Thus, it is an essential task to identify HPTM readers for understanding of epigenetic regulation. Herein we designed and prepared a novel HPTM probe based on self-assembled multivalent photo-cross-linking technique for selective enrichment and identification of HPTM readers...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30184427/fabrication-of-liquid-crystal-based-optical-sensing-platform-for-detection-of-hydrogen-peroxide-and-blood-glucose
#17
Lubin Qi, Qiongzheng Hu, Qi Kang, Li Yu
Rapid and accurate determination of H2 O2 is of great importance in practical applications. In this study, we demonstrate construction of liquid-crystal (LC)-based sensing platforms for sensitive and real-time detection of H2 O2 with high accuracy for the first time. Single-stranded DNA (ssDNA) adsorbed onto the surface of nanoceria is released to the aqueous solution in the presence of H2 O2 , which disrupts arrangement of the self-assembled cationic surfactant monolayer decorated at the aqueous/LC interface...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30226760/allele-specific-isothermal-amplification-method-using-novel-unmodified-self-stabilizing-competitive-primers
#18
Kenny Malpartida-Cardenas, Jesus Rodriguez-Manzano, Ling-Shan Yu, Michael Delves, Chea Nguon, Kesinee Chotivanich, Jake Baum, Pantelis Georgiou
Rapid and specific detection of single nucleotide polymorphisms (SNPs) related to drug resistance in infectious diseases is crucial for accurate prognostics, therapeutics and disease management at point-of-care. Here, we present a novel amplification method and provide universal guidelines for the detection of SNPs at isothermal conditions. This method, called USS-sbLAMP, consists of SNP-based loop-mediated isothermal amplification (sbLAMP) primers and unmodified self-stabilizing (USS) competitive primers that robustly delay or prevent unspecific amplification...
September 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30226755/a-multi-channel-structured-3d-chip-for-highly-sensitive-pathogenic-bacteria-detection-based-on-fast-dna-programmed-signal-polymerization
#19
Junman Chen, Zhijun Huang, Zewei Luo, Qiaoling Yu, Ya Xu, Xiaqing Wang, Yongxin Li, Yixiang Duan
The threat of food safety and the limited analytical methods with high-performance promote the growing interest in the development of pathogenic bacteria biosensors. This study presents a pathogenic bacteria biosensing system, where a novel three-dimensional (3D) chip acts as analytical carrier and DNA-programmed hybridization chain reaction (HCR) causes signal amplification. The 3D chip is designed featuring a compact multi-channel structure. It has large surface area for sensitive sensing, and exhibits multiple functions of target capture, separation, rinse and signal detection to simplify the analysis processes...
September 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30226366/screening-estrogen-receptor-modulators-in-a-paper-based-breast-cancer-model
#20
Nathan A Whitman, Zhi-Wei Lin, Thomas J DiProspero, Julie C McIntosh, Matthew R Lockett
The health risks associated with acute and prolonged exposure to estrogen receptor (ER) modulators has led to a concerted effort to identify and prioritize potential disruptors present in the environment. ER agonists and antagonists are identified with end-point assays, quantifying changes in cellular proliferation or gene transactivation in monolayers of estrogen receptor alpha expressing (ER+) cells upon exposure. While these monolayer cultures can be prepared, dosed, and analyzed in a highly parallelized manner, they are unable to predict the potencies of ER modulators in vivo accurately...
September 18, 2018: Analytical Chemistry
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