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Analytica Chimica Acta

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https://www.readbyqxmd.com/read/28693734/hydrophilic-interaction-liquid-chromatography-mass-spectrometry-as-a-new-tool-for-the-characterization-of-intact-semi-synthetic-glycoproteins
#1
Sara Tengattini, Elena Domínguez-Vega, Caterina Temporini, Teodora Bavaro, Francesca Rinaldi, Luciano Piubelli, Loredano Pollegioni, Gabriella Massolini, Govert W Somsen
Improved methods for detailed characterization of complex glycoproteins are required in the growing sector of biopharmaceuticals. Hydrophilic interaction liquid chromatography (HILIC) coupled to high resolution (HR) time-of-flight mass spectrometric (TOF-MS) detection was examined for the characterization of intact neo-glycoproteins prepared by chemical conjugation of synthetic saccharides to the lysine residues of selected recombinant proteins. The separation performances of three different amide HILIC columns (TSKgel Amide-80, XBridge BEH and AdvanceBio Glycan Mapping) were tested...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693733/a-fluorescent-dyad-with-large-emission-shift-for-discrimination-of-cysteine-homocysteine-from-glutathione-and-hydrogen-sulfide-and-the-application-of-bioimaging
#2
Xueling Yang, Longwei He, Kaixin Xu, Weiying Lin
Biothiols, as reactive sulfur species (RSS), play important roles in human physiology, and they have a close connection of generation and metabolism pathways among of them. It is challenging to discriminate biothiols from each other due to the similar chemical structures and properties of them. Herein, we develop a fluorescent hybrid dyad (CS-NBD) for efficiently discriminating cysteine (Cys)/homocysteine (Hcy) from glutathione (GSH) and hydrogen sulfide (H2S) by a dual-channel detection method. CS-NBD performs inherently no fluorescence in ranging from visible to near infrared region...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693732/wheat-germ-agglutinin-and-lens-culinaris-agglutinin-sensitized-anisotropic-silver-nanoparticles-in-detection-of-bacteria-a-simple-photometric-assay
#3
Mariam V Mikaelyan, Gayane G Poghosyan, Olga D Hendrickson, Boris B Dzantiev, Vardan K Gasparyan
Efficacy of anisotropic silver nanoparticles sensitized with wheat germ agglutinin (WGA) and Lens culinaris agglutinin (LCA) was studied for detection of Staphylococcus aureus and Escherichia coli. It was demonstrated that interaction of these nanoparticles with bacteria stabilizes them and prevents their aggregation upon addition of sodium chloride; such stabilization depends on bacteria concentration. High concentration of bacteria results in higher stabilization whereas low concentration leads to aggregation of nanoparticles...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693731/simple-area-determination-of-strongly-overlapping-ion-mobility-peaks
#4
Lucie Borovcová, Martina Hermannová, Volodymyr Pauk, Matěj Šimek, Vladimír Havlíček, Karel Lemr
Coupling of ion mobility with mass spectrometry has brought new frontiers in separation and quantitation of a wide range of isobaric/isomeric compounds. Ion mobility spectrometry may separate ions possessing the identical molecular formula but having different molecular shapes. The separation space in most commercially available instruments is limited and rarely the mobility resolving power exceeds one hundred. From this perspective, new approaches allowing for extracting individual compound signals out of a more complex mixture are needed...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693730/chiral-differentiation-of-amino-acids-through-binuclear-copper-bound-tetramers-by-ion-mobility-mass-spectrometry
#5
Xiangying Yu, Zhong-Ping Yao
In this study, travelling wave ion mobility mass spectrometry has been used to investigate chiral differentiation of common chiral amino acids, through separation of binuclear copper bound tetrameric ions formed with chiral selector tryptophan, proline, tyrosine, phenylalanine or histidine. Significant chiral recognition was observed for amino acids with aromatic rings or long and active side chains, with peak-to-peak resolutions up to 1.826. Tryptophan and histidine offered better enantioselectivity, compared to other chiral selectors...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693729/sialic-acid-linkage-specific-permethylation-for-improved-profiling-of-protein-glycosylation-by-maldi-tof-ms
#6
Kuan Jiang, He Zhu, Lei Li, Yuxi Guo, Ebtesam Gashash, Cheng Ma, Xiaolin Sun, Jing Li, Lianwen Zhang, Peng George Wang
Protein glycosylation mediates a wide range of cellular processes, affecting development and disease in mammals. Deciphering the "glycocodes" requires rapid, sensitive and in-depth characterization of diverse glycan structures derived from biological samples. In this study, we described a two-step derivatization strategy termed linkage-specific sialic acid permethylation (SSAP) consisting of dimethylamination and permethylation for the improved profiling of glycosylation by matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS)...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693728/electrochemical-detection-of-cardiac-biomarker-myoglobin-using-polyphenol-as-imprinted-polymer-receptor
#7
J A Ribeiro, C M Pereira, A F Silva, M Goreti F Sales
An electrochemical biosensor was developed by merging the features of Molecular Imprinting technique and Screen-Printed Electrode (SPE) for the simple and fast screening of cardiac biomarker myoglobin (Myo) in point-of-care (POC). The MIP artificial receptor for Myo was prepared by electrooxidative polymerization of phenol (Ph) on a AuSPE in the presence of Myo as template molecule. The choice of the most effective protein extraction procedure from the various extraction methods tested (mildly acidic/basic solutions, pure/mixed organic solvents, solutions containing surfactants and enzymatic digestion methods), and the optimization of the thickness of the polymer film was carefully undertaken in order to improve binding characteristics of Myo to the imprinted polymer receptor and increase the sensitivity of the MIP biosensor...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693727/one-step-synthesis-of-functional-pnr-rgo-composite-as-a-building-block-for-enhanced-ascorbic-acid-biosensing
#8
Ling Liu, Junfeng Zhai, Chengzhou Zhu, Lei Han, Wen Ren, Shaojun Dong
An electrochemical sensor for ascorbic acid (AA) was prepared via an one-step electrochemical approach by reducing graphene oxide (rGO) and co-polymerizing neutral red (NR) and rGO to form a pNR/rGO hybrid film on the glassy carbon electrode (pNR/rGO-GCE). Structures and properties of the obtained pNR/rGO film were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) and UV-vis techniques. A significant decrease of charge-transfer resistance (Rct) from over 20,000 Ω for pNR-GCE to 130 Ω for pNR/rGO-GCE was validated by electrochemical impedance spectroscopy (EIS) measurement...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693726/point-of-need-simultaneous-electrochemical-detection-of-lead-and-cadmium-using-low-cost-stencil-printed-transparency-electrodes
#9
Daniel Martín-Yerga, Isabel Álvarez-Martos, M Carmen Blanco-López, Charles S Henry, M Teresa Fernández-Abedul
In this work, we report a simple and yet efficient stencil-printed electrochemical platform that can be integrated into the caps of sample containers and thus, allows in-field quantification of Cd(II) and Pb(II) in river water samples. The device exploits the low-cost features of carbon (as electrode material) and paper/polyester transparency sheets (as substrate). Electrochemical analysis of the working electrodes prepared on different substrates (polyester transparency sheets, chromatographic, tracing and office papers) with hexaammineruthenium(III) showed that their electroactive area and electron transfer kinetics are highly affected by the porosity of the material...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693725/impact-of-%C3%AE-irradiation-ageing-and-their-interactions-on-multilayer-films-followed-by-acomdim
#10
Fanny Gaston, Nathalie Dupuy, Sylvain R A Marque, Magali Barbaroux, Samuel Dorey
To highlight the main factors involved in the degradation of polymers in multilayer films under γ-irradiation, the ANOVA Common Dimensions (AComDim, Analysis of Variance in Common Dimensions) method is applied on spectra recorded with ATR-FTIR (Attenuated Total Reflection-Fourier Transform Infrared). The present study focuses on the stability of γ-irradiated polymers used in single-use plastic bags made of multilayer films for the biopharmaceutical and biotechnological industries. The samples are irradiated at several γ-doses, up to 270 kGy, and compared with a non-irradiated sample used as reference...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693724/direct-coupling-of-supercritical-fluid-chromatography-with-tandem-mass-spectrometry-for-the-analysis-of-amino-acids-and-related-compounds-comparing-electrospray-ionization-and-atmospheric-pressure-chemical-ionization
#11
Denise Wolrab, Peter Frühauf, Christopher Gerner
For acceptance of supercritical fluid chromatography (SFC) as a routine analysis method, the hyphenation to mass spectrometry (MS), which is typically achieved either by a splitting device or by the employment of an additional make up flow, has to be improved. Direct coupling of SFC to MS (/MS) would simplify the handling of this method. Consequently, this work focused on the direct coupling of SFC to mass spectrometry and the influence of the employed ion source on signal intensities of polar and ionic compounds in biological samples...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28693723/determination-of-formylated-dna-and-rna-by-chemical-labeling-combined-with-mass-spectrometry-analysis
#12
Han-Peng Jiang, Ting Liu, Ning Guo, Lei Yu, Bi-Feng Yuan, Yu-Qi Feng
Nucleic acids carry diverse chemical modifications that exert critical influences in a variety of cellular processes in living organisms. In addition to methylation, the emerging DNA and RNA formylation has been reported to play functional roles in various physiological processes. However, the amounts of formylated DNA and RNA are extremely low and detection of DNA and RNA formylation is therefore a challenging task. To address this issue, we developed a strategy by chemical labeling combined with in-tube solid-phase microextraction - ultra high performance liquid chromatography - electrospray ionization - tandem mass spectrometry (in-tube SPME-UPLC-ESI-MS/MS) analysis for the sensitive determination of DNA and RNA formylation...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622806/graphitic-carbon-nitride-supported-platinum-nanocomposites-for-rapid-and-sensitive-colorimetric-detection-of-mercury-ions
#13
Yi-Wei Wang, Lixing Wang, Fengping An, Hui Xu, Zejin Yin, Shurong Tang, Huang-Hao Yang, Hongbo Song
In this study, graphitic carbon nitride supported platinum nanocomposites (g-C3N4/PtNPs) have been synthesized for the first time by an ultrasonic-assisted chemical reduction method. By using g-C3N4 as the stabilizer, Pt ions could be reduced to PtNPs by NaBH4 and uniformly deposited on the surface of g-C3N4. The resulting g-C3N4/PtNPs exhibited enhanced catalytic activity for the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) compared to that of g-C3N4 or PtNPs alone. After interaction with Hg(2+), the catalytic activity of g-C3N4/PtNPs was effectively inhibited due to the formation of HgPt amalgam...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622805/nanomolar-sensitive-colorimetric-assay-for-mn-2-using-cysteic-acid-capped-silver-nanoparticles-and-theoretical-investigation-of-its-sensing-mechanism
#14
Yan-Xia Qi, Zhi-Bei Qu, Qi-Xian Wang, Min Zhang, Guoyue Shi
A new facile, rapid, sensitive and selective colorimetric assay is proposed for the determination of manganese ions (Mn(2+)) utilizing cysteic acid (CA)-capped silver nanoparticles (CA-AgNPs) as colorimetric probes. The CA-AgNPs were prepared by reducing AgNO3 with NaBH4 in the presence of CA as the capping ligand. The presence of Mn(2+) induces the quick aggregation of CA-AgNPs, associated with notable color changes of the CA-AgNPs solution from yellow to dark green. The Mn(2+) can form a coordinated structure with CA capping on the AgNPs and leads to formation of large particles aggregation...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622804/g-quadruplex-based-exo-iii-assisted-signal-amplification-aptasensor-for-the-colorimetric-detection-of-adenosine
#15
Lei Xu, Xin Shen, Bingzhi Li, Chunhong Zhu, Xuemin Zhou
Adenosine is an endogenous nucleotide pivotally involved in nucleic acid and energy metabolism. Its excessive existence may indicate tumorigenesis, typically lung cancer. Encouraged by its significance as the clinical biomarker, sensitive assay methods towards adenosine have been popularized, with high cost and tedious procedures as the inevitable defects. Herein, we report a label-free aptamer-based exonuclease III (Exo III) amplification colorimetric aptasensor for the highly sensitive and cost-effective detection of adenosine...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622803/lab-on-paper-chip-integrated-with-si-gnrs-for-electroanalysis-of-diazepam
#16
Jagriti Narang, Chaitali Singhal, Ashish Mathur, Manika Khanuja, Ankur Varshney, Kartikey Garg, Tulika Dahiya, C S Pundir
We describe herein the fabrication of an electrochemical microfluidic paper based device (EμPAD) for the detection of diazepam, a sedative, anxiety-relieving and muscle-relaxing drug. To achieve it, silica coated gold nanorods (Si@GNRs) were synthesized and drop casted on an electrochemical microfluidic paper based device (EμPAD) for the detection of diazepam. The synthesized composites were characterized by recording its images in scanning electron microscope (SEM) and transmission electron microscope (TEM)...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622802/rapid-multiplex-dna-amplification-on-an-inexpensive-microdevice-for-human-identification-via-short-tandem-repeat-analysis
#17
Jacquelyn A DuVall, Delphine Le Roux, Brandon L Thompson, Christopher Birch, Daniel A Nelson, Jingyi Li, Daniel L Mills, An-Chi Tsuei, Martin G Ensenberger, Cindy Sprecher, Douglas R Storts, Brian E Root, James P Landers
Forensic DNA analysis requires several steps, including DNA extraction, PCR amplification, and separation of PCR fragments. Intuitively, there are numerous situations where it would be beneficial to speed up the overall DNA analysis process; in this work, we focus on the most time-consuming component in the analysis pipeline, namely the polymerase chain reaction (PCR). Primers were specially designed to target 10 human genomic loci, all yielding amplicons shorter than 350 bases, for ease of downstream integration with on-board microchip electrophoresis...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622801/rapid-determination-of-nitrate-in-vegetables-by-gas-chromatography-mass-spectrometry
#18
Beatrice Campanella, Massimo Onor, Enea Pagliano
In this study we present a novel isotope dilution gas chromatography method for the determination of nitrate in vegetables. The analyte was extracted in water at 70(∘)C and mixed with (15)N isotopically enriched nitrate internal standard. The sample was centrifuged and the supernatant reacted with sulfamic acid for removal of nitrite, and with triethyloxonium tetrafluoroborate for converting nitrate into volatile EtONO2. This simple aqueous chemistry allowed for separation of analyte from sample matrix in the form of a gaseous derivative which could be sampled in the headspace before GC-MS analysis...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622800/analysis-of-stable-isotope-assisted-metabolomics-data-acquired-by-gc-ms
#19
Xiaoli Wei, Biyun Shi, Imhoi Koo, Xinmin Yin, Pawel Lorkiewicz, Hamid Suhail, Ramandeep Rattan, Shailendra Giri, Craig J McClain, Xiang Zhang
Stable isotope assisted metabolomics (SIAM) measures the abundance levels of metabolites in a particular pathway using stable isotope tracers (e.g., (13)C, (18)O and/or (15)N). We report a method termed signature ion approach for analysis of SIAM data acquired on a GC-MS system equipped with an electron ionization (EI) ion source. The signature ion is a fragment ion in EI mass spectrum of a derivatized metabolite that contains all atoms of the underivatized metabolite, except the hydrogen atoms lost during derivatization...
August 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28622799/quantitative-nmr-analysis-of-intra-and-extracellular-metabolism-of-mammalian-cells-a-tutorial
#20
REVIEW
Sarantos Kostidis, Ruben D Addie, Hans Morreau, Oleg A Mayboroda, Martin Giera
Metabolomics analysis of body fluids as well as cells is depended on many factors. While several well-accepted standard operating procedures for the analysis of body fluids are available, the NMR based quantitative analysis of cellular metabolites is less well standardized. Experimental designs depend on the cell type, the quenching protocol and the applied post-acquisition workflow. Here, we provide a tutorial for the quantitative description of the metabolic phenotype of mammalian cells using NMR spectroscopy...
August 8, 2017: Analytica Chimica Acta
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