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Analytica Chimica Acta

Jingjing Deng, Guoyue Shi, Tianshu Zhou
This study demonstrates a new strategy for colorimetric detection of alcoholic strength (AS) in spirit samples based on dual-responsive lanthanide infinite coordination polymer (Ln-ICP) particles with ratiometric fluorescence. The ICP used in this study are composed of two components: one is the supramolecular Ln-ICP network formed by the coordination between the ligand 2,2'-thiodiacetic acid (TDA) and central metal ion Eu(3+); and the other is a fluorescent dye, i.e., coumarin 343 (C343), both as the cofactor ligand and as the sensitizer, doped into the Ln-ICP network through self-adaptive chemistry...
October 26, 2016: Analytica Chimica Acta
Ziping Liu, Xingguang Su
Herein, high quality DNA-CuInS2 QDs are facilely synthesized through a one-pot hydrothermal method with fluorescence quantum yield as high as 23.4%, and the strongly fluorescent DNA-CuInS2 QDs have been utilized as a novel fluorescent biosensor for label-free and ultrasensitive detection of anthrax lethal factor DNA. L-Cysteine (L-Cys) and a specific-sequence DNA are used as co-ligands to stabilize the CuInS2 QDs. The specific-sequence DNA consists of two domains: phosphorothiolates domain (sulfur-containing variants of the usual phosphodiester backbone) controls the nanocrystal passivation and serves as a ligand, and the functional domain (non-phosphorothioates) controls the biorecognition...
October 26, 2016: Analytica Chimica Acta
S F A Rahman, N A Yusof, U Hashim, R Hushiarian, M Nuzaihan M N, M N Hamidon, R M Zawawi, M F M Fathil
Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis...
October 26, 2016: Analytica Chimica Acta
Dan Wu, Tao Gao, Lin Lei, Dawei Yang, Xiaoxia Mao, Genxi Li
The detection of protein is vital to fundamental research as well as practical applications. However, most detection methods depend on antibody-based assays which are faced with many shortcomings. Herein, we propose a colorimetric method for protein assays based on target-triggered activation of aptazyme, which may offer simple, rapid and cost-effective detection of the target protein. In this method, the conformation change of aptazyme induced by target protein is designed to be associated with aptazyme activation...
October 26, 2016: Analytica Chimica Acta
Athina S Kastania, Katerina Tsougeni, George Papadakis, Electra Gizeli, George Kokkoris, Angeliki Tserepi, Evangelos Gogolides
We present a polymeric microfluidic chip capable of purifying DNA through solid phase extraction. It is designed to be used as a module of an integrated Lab-on-chip platform for pathogen detection, but it can also be used as a stand-alone device. The microfluidic channels are oxygen plasma micro-nanotextured, i.e. randomly roughened in the micro-nano scale, a process creating high surface area as well as high density of carboxyl groups (COOH). The COOH groups together with a buffer that contains polyethylene glycol (PEG), NaCl and ethanol are able to bind DNA on the microchannel surface...
October 26, 2016: Analytica Chimica Acta
Minzhi Peng, Yanna Cai, Xiefan Fang, Li Liu
A novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been established to quantify metabolic intermediates, including lactate (Lac), pyruvate (Pyr), acetoacetate (ACAC) and 3-hydroxybutyrate (3-HB) in blood. Samples were deproteinized with methanol-acetonitrile solution, and analytes were separated on an adamantyl group-bonded reverse phase column and detected in multiple reaction monitoring mode. Total analysis time was 4 min per sample. Method validation results displayed that limits of quantification were 10...
October 26, 2016: Analytica Chimica Acta
Hanno Evard, Anneli Kruve, Ivo Leito
In part II of this tutorial, the investigated approaches of estimating the limit of detection (LOD) are applied to experimental data from LC-MS measurements. Important practical aspects specific to LC-MS and related to LOD are reviewed. The results of different tests of estimating linearity and scedasticity are compared. LOD estimates obtained with different approaches (for both simple characterization of the analysis method and accurate interpretation of the results) are applied to the data and the obtained values are compared...
October 26, 2016: Analytica Chimica Acta
Hanno Evard, Anneli Kruve, Ivo Leito
A large body of literature exists on the limit of detection (LOD), but there is still a lot of confusion about this important validation parameter. This confusion mainly stems from its statistically complex background. The goal of this two-part tutorial is to discuss and clarify the topic of LOD for practitioners. The two main conclusions of this tutorial are: (1) the choice of how to estimate LOD should be based on the purpose of the analytical method that is being validated (e.g. considerable effort should not be made to estimate LOD for a method that is not used for detecting traces in the vicinity of LOD), and (2) LOD estimates are strongly dependent on different assumptions and the approach used, and therefore caution must be exercised when using the estimate or when comparing different estimates...
October 26, 2016: Analytica Chimica Acta
Urh Černigoj, Jernej Gašperšič, Andreas Fichtenbaum, Nika Lendero Krajnc, Jana Vidič, Goran Mitulović, Aleš Štrancar
Metal oxide affinity chromatography has been one of the approaches for specific enrichment of phosphopeptides from complex samples, based on specific phosphopeptide adsorption forming bidentate chelates between phosphate anions and the surface of a metal oxide, such as TiO2, ZrO2, Fe2O3, and Al2O3. Due to convective mass transfer, flow-independent resolution and high dynamic binding capacity, monolith chromatographic supports have become important in studies where high resolution and selectivity are required...
October 26, 2016: Analytica Chimica Acta
Petr Tůma, Martin Jaček, Vladimíra Fejfarová, Jan Polák
An electrophoretic stacking method has been developed for monitoring the therapeutic level of the antibiotic ceftazidime in blood plasma and microdialysates taken from peripheral soft tissues of the lower limbs of patients with diabetic foot syndrome. The biological samples are treated by addition of acetonitrile in an amount of 75% v/v and injected into a capillary in a large volume; after turning on the separation voltage, the residual acetonitrile is forced out of the capillary by the application of hydrodynamic pressure...
October 26, 2016: Analytica Chimica Acta
G P Danezis, C J Anagnostopoulos, K Liapis, M A Koupparis
One of the recent trends in Analytical Chemistry is the development of economic, quick and easy hyphenated methods to be used in a field that includes analytes of different classes and physicochemical properties. In this work a multi-residue method was developed for the simultaneous determination of 28 xenobiotics (polar and hydrophilic) using hydrophilic interaction liquid chromatography technique (HILIC) coupled with triple quadrupole mass spectrometry (LC-MS/MS) technology. The scope of the method includes plant growth regulators (chlormequat, daminozide, diquat, maleic hydrazide, mepiquat, paraquat), pesticides (cyromazine, the metabolite of the fungicide propineb PTU (propylenethiourea), amitrole), various multiclass antibiotics (tetracyclines, sulfonamides quinolones, kasugamycin and mycotoxins (aflatoxin B1, B2, fumonisin B1 and ochratoxin A)...
October 26, 2016: Analytica Chimica Acta
Elizabeth S Gilchrist, David A Healy, Virginia N Morris, Jeremy D Glennon
This paper is a review of ion chromatographic (IC) separations of inorganic oxyhalide disinfection by-products (DBPs) in water and beverages. The review outlines the chemical mechanisms of formation, regulation of maximum allowable levels, chromatographic column selection and speciation. In addition, this review highlights the application of IC coupled to mass spectrometry (MS) for trace and elemental composition analysis of oxyhalides, along with the analytical considerations associated to enable sensitive analysis...
October 26, 2016: Analytica Chimica Acta
Xinrong Guo, Fangying Wu, Yongnian Ni, Serge Kokot
A strong red fluorescent nanocomposite, consisting of graphite-like carbon nitride nanosheets (g-C3N4 NSs) and serum albumin-capped Au nanoclusters (AuNCs), was synthesized. Dopamine (DA) can quench the red fluorescence of the nanocomposite, based on the Forster resonance energy transfer (FRET) mechanism. In this quenching process, the energy is transferred from the fluorescent g-C3N4 NSs-AuNCs to the oxidized DA quinine molecules (DA is easily oxidated to form DA quinine in air). The red fluorescence emission at 420 nm decreases dramatically and the quenching ratio (F0 - F)/F0 is linearly related to the concentration of DA in the range of 0...
October 26, 2016: Analytica Chimica Acta
Yunqiang Tang, Jinguo Sun, Bingzhu Yin
A Schiff base-type fluorescent probe (1) consist of 2-hydroxynaphthaldehyde and glutamide moieties has been designed and synthesized for detection Zn(2+) and Al(3+). The probe shows pH dependent dual-selectivity for Zn(2+) and Al(3+) in Tris-HCl buffer, viz. that can selectively recognized Zn(2+) at pH 7.4 and Al(3+) at pH 6.0, respectively. From Job's plots and MS data, the stoichiometric ratios of the probe with Zn(2+) and Al(3+) appeared to be 1:1 and 2:1, respectively. The probe can detect as low as 5.5 × 10(-8) M(-1) Zn(2+) and 1...
October 26, 2016: Analytica Chimica Acta
Weifeng Shen, Wei Han, Yunong Li, Zhiqi Meng, Leiming Cai, Liang Li
Silkworm (Bombyx mori) is a very useful target insect for evaluation of endocrine disruptor chemicals (EDCs) due to mature breeding techniques, complete endocrine system and broad basic knowledge on developmental biology. Comparative metabolomics of silkworms with and without EDC exposure offers another dimension of studying EDCs. In this work, we report a workflow on metabolomic profiling of silkworm hemolymph based on high-performance chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) and demonstrate its application in studying the metabolic changes associated with the pesticide dichlorodiphenyltrichloroethane (DDT) exposure in silkworm...
October 26, 2016: Analytica Chimica Acta
Shuhuai Li, Xuejin Wu, Chunhua Liu, Guihao Yin, Jinhui Luo, Zhi Xu
In this study, an electrogenerated chemiluminescence (ECL) sensing platform for carbofuran detection was constructed based on ECL energy transfer (ECRET) and carbon dot (C-dot)-tagged aptamers as the recognition element. Fullerene (C60)-loaded gold nanoparticles (C60-Au) were used as the energy donor, modified on a glassy carbon electrode. C-dot-tagged DNA aptamers were used as the receptor, and ECRET then occurred between C60-Au and C-dots. After accepting the energy, the C-dots acted as a signal indicator and showed decreased signal intensity in the presence of targets, which competitively bound to DNA aptamers and blocked energy transfer...
October 19, 2016: Analytica Chimica Acta
Aixian Zheng, Xiaolong Zhang, Ju Gao, Xiaolong Liu, Jingfeng Liu
Glypican-3 (GPC3) might be used as new biomarker of liver cancer for the development of new diagnostic methods. The most commonly used methods for protein detection are based on natural enzymes, which are easily affected by environmental conditions and suffer from the rigorous preparation conditions. Thus, the development of new enzyme mimetics with high and stable catalytic activity is of great significance in diagnostic applications. In this paper, copper ions (Cu(2+)) was found to possess the peroxidase-like catalytic activity, which can catalyze H2O2-mediated oxidation of peroxidase substrate and obtain the oxidation product with color change...
October 19, 2016: Analytica Chimica Acta
Li Gao, Qin Li, Raoqi Li, Zebin Deng, Brendan Brady, Ni Xia, Guimin Chen, Yang Zhou, Hengchuan Xia, Keping Chen, Haixia Shi
Recently, graphene oxide (GO) has shown superiority for disease detection arising from its unique physical and chemical properties. However, proteins adsorbed on the surface of GO prevent sensitivity improvement in fluorescence-based detection methods. In this paper, a label-free method based on aptamer modified gold nanoparticles (GNPs) combined with Tween 80 was shown to solve this problem using the detection of thrombin as an example. An aptamer was designed and bound to thrombin by changing its conformation...
October 19, 2016: Analytica Chimica Acta
S Prati, M Milosevic, G Sciutto, I Bonacini, S G Kazarian, R Mazzeo
The identification of organic dyes is a challenging task in all the fields such as the forensic and conservation sciences, especially in cases where the amount of sample is extremely small. In this paper we propose a new enhanced FTIR method (MU-ATR metal underlayer ATR spectroscopy), which we believe is the first of its kind, for the analysis of a few ng of dyes. With this method, dyed fiber micro-extracts can be analyzed using a commercial FTIR microscope with a fixed incident angle, obtaining the same separation between the different classes of dyes investigated as we obtained analyzing pure dyes in transmission mode...
October 19, 2016: Analytica Chimica Acta
K Udeni Alwis, T Liz Bailey, Dhrusti Patel, Liqun Wang, Benjamin C Blount
Isoprene, the 2-methyl analog of 1,3-butadiene, is identified as a possible human carcinogen by the International Agency for Research on Cancer (IARC). Isoprene is ubiquitous in the environment with numerous natural and anthropogenic sources. Tobacco smoke is the main exogenous source of isoprene exposure in indoor environments. Among smoke constituents, isoprene is thought to contribute significantly to cancer risk; however, no selective urinary biomarkers of isoprene exposure have been identified for humans...
October 19, 2016: Analytica Chimica Acta
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