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Analytica Chimica Acta

Kunhao Yang, Zhongqiu Li, Yueguang Lv, Chuyi Yu, Peilong Wang, Xiaoou Su, Li Wu, Yujian He
An electrochemical aptasensor for ultrasensitive detection of hydroxylated polychlorinated biphenyl (OH-PCB) was developed. In this work, the sulfydryl aptamer selected for OH-PCB was easily immobilized on the surface of the modified glassy carbon electrode via Au-S bond due to incorporation of gold nanoparticles/poly dimethyl diallyl ammonium chloride-graphene composite. The fabrication process of the electrochemical aptasensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry...
December 24, 2018: Analytica Chimica Acta
Angéline Kernalléguen, Christine Enjalbal, Jean-Claude Alvarez, Omar Belgacem, Georges Léonetti, Daniel Lafitte, Anne-Laure Pélissier-Alicot
New designer drugs, as synthetic cannabinoids (SCs), continuously appear on the market and are booming due to their cannabis-like effect. New generation of smokable SCs, structurally dissimilar from Δ9-tetrahydrocannabinol (THC), have isomers with distinguishable pharmacokinetic parameters and therefore different in vivo effects. The isoforms are misidentified using conventional techniques such as gas or liquid chromatography coupled to mass spectrometry - or tandem mass - spectrometry. The aim of this study was to differentiate three positional isomers (JWH-007, JWH-019 and JWH-122) in single human hair samples, which store numerous substances revealing a way of life and consumption style...
December 24, 2018: Analytica Chimica Acta
Ge Ma, Xiaoyong Zhao, Cheng Guo, Shaojia Li, Yaqin Liu, Quan He, Kunsong Chen, Yuanjiang Pan
Current imaging techniques, such as position emission tomography, magnetic resonance imaging and optical imaging, have been developed and applied to imaging acidity in live tissues for years, but they still have some limitations. In the present study, a reliable and interference-free pH imaging method based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was established for the first time. A novel reactive matrix, named glycosyl-3-aminoquinoline (Gly-3AQ), was designed and synthesized here not only to overcome the background interference in the low mass range of MALDI but also provide a new function of acid-responsiveness for the matrix...
December 24, 2018: Analytica Chimica Acta
Iris S Denmark, Ermira Begu, Zikri Arslan, Fengxiang X Han, Jennifer M Seiter-Moser, Eric M Pierce
A procedure is developed for selective extraction of methylmercury (CH3 Hg+ ) from heavily Hg-contaminated soils and sediments for determination by chemical vapor generation inductively coupled plasma mass spectrometry (CVG-ICP-MS). Soils artificially contaminated with 40 μg g-1 inorganic mercury (Hg2+ ) or methylmercury chloride (CH3 HgCl) were agitated by shaking or exposing to ultrasounds in dilute hydrochloric acid (HCl) or nitric acid (HNO3 ) solutions at room temperature. Extractions in HCl (5 or 10% v/v) resulted in substantial leaching of Hg2+ from soils, whereas 5% (v/v) HNO3 provided selectivity for quantitative extraction of CH3 Hg+ with minimum Hg2+ leaching...
December 24, 2018: Analytica Chimica Acta
Yuantao Zhou, Shurui Cao, Cunxian Xi, Guoyin Lai, Xianliang Li, Lei Zhang, Guomin Wang, Zhiqiong Chen
Highly graphitic nanoporous carbon (NPC) was obtained from an agricultural waste-citrus peel at optimum condition. Then, a low-cost and pore size-controlled magnetic graphitic nanoporous carbon (MNPC) with ultrahigh specific surface area (1064 m2  g-1 ) was successfully fabricated by coprecipitation of Fe3 O4 particles onto NPC. The prepared MNPC was characterized by Brunauer-Emmett-Teller (BET), Raman spectrum, scanning electron microscope (SEM) and Fourier transform infrared spectrometry (FT-IR). BET results showed a significant increase in the micropores volume and specific surface area of MNPCs following the increase of carbonization temperature, which implied that highly efficient carbonization made NPC an excellent active phase for MNPC...
December 24, 2018: Analytica Chimica Acta
Paulo T Garcia, Anderson A Dias, João A C Souza, Wendell K T Coltro
This study describes, for the first time, the use of a batch injection analysis system with amperometric detection (BIA-AD) to indirectly determine salivary α-amylase (sAA) levels in saliva samples for chronic periodontitis diagnosis. A chemical/thermal treatment was explored to generate a CuO film on a Cu electrode surface. This procedure offered good stability (RSD = 0.3%), good repeatability (RSD < 1.3%) and excellent reproducibility (RSD < 1.5%). The sAA concentration levels were determined based on the detection of maltose produced by enzymatic hydrolysis of starch...
December 24, 2018: Analytica Chimica Acta
R Nazari-Vanani, N Sattarahmady, H Yadegari, N Delshadi, G R Hatam, H Heli
Detecting and monitoring the pathogens with high selectivity and sensitivity is critical for public health. In the present study, we demonstrated a specific analytical strategy for sensitive detection of Leishmania infantum genome. The developed sensor utilized toluidine blue as a hybridization indicator and a Leishmania infantum-specific capture DNA sequence immobilized on a high-surface area gold nanostructure as an electrochemical transducer. The produced analytical response was based on the hybridization of the single-stranded DNA from the target with the immobilized DNA sequence at the electrode surface...
December 24, 2018: Analytica Chimica Acta
Wajee Jaikaew, Adrian Ruff, Panida Khunkaewla, Thomas Erichsen, Wolfgang Schuhmann, Albert Schulte
Robotic square wave voltammetry (SVW) in 24-well microtiter plates has been developed as a reliable non-manual procedure for quantifying drug release from pharmaceutical hydrogels. The assay was established using 1% agarose disks containing Paracetamol® (PCT) as a model preparation. Computerized buffer delivery and SVW in calibration and hydrogel sample wells were performed by a three-electrode arrangement combined with a thin plastic tube. For the glassy carbon working electrode of the assembly the upper limit of the linear response and the lower detection limit of sequential 'in-well' PCT-SVW were 1000 and 0...
December 24, 2018: Analytica Chimica Acta
Ping Li, Hua Zhang, Dong Wang, Yingjie Tao, Lun Zhang, Wenchao Zhang, Xudong Wang
A sensitive and highly efficient approach to monitor the expression of proteins on live cells was urgently needed to demonstrate its factor and mechanism and most important for clinical diagnostics and molecular biology. Herein, we developed a simple and highly efficient strategy, nonlinear hybridization chain reaction (nonlinear HCR), for the sensitive determination of proteins on live cells with transient receptor potential vanilloid 4 (TRPV4) and RAW264.7 cells as a model. Unlike the normal hybridization chain reaction (HCR) with multiplicative amplification, an exponential amplified fluorescent response could be obtained in theory based on the proposed nonlinear HCR...
December 24, 2018: Analytica Chimica Acta
Kornelia Poniedziałek, Katarzyna Bielicka-Daszkiewicz
In this work, the microextraction devices in form of sachets were prepared and used for isolation of released dental fillings components from artificial saliva solutions As sorbents mesoporous aluminosilicate and C18-bonded silica were used. Gas chromatography was used for indication of analytes in samples. The dependence of adsorption on variable factors were determined. The effect of adsorbent mass, the membrane type for μ-SPE device and time of adsorption on the efficiency of the process were examined. The dependence between the extraction recovery and the desorption solvent was also indicated...
December 24, 2018: Analytica Chimica Acta
S Cersoy, G Daheur, A Zazzo, S Zirah, M Sablier
Exogenous carbonaceous contaminants coming from sediments significantly bias the radiocarbon date of collagen samples extracted from archaeological bone and teeth. In this study, a new approach combining pyrolysis, comprehensive gas chromatography and mass spectrometry (Py-GC × GC/MS) was proposed to ensure their removal during the demineralization and bone collagen extraction. This approach permitted to identify hydrocarbon contaminants for archaeological samples from the Neolithic period, in 30-40 μg of collagen...
December 24, 2018: Analytica Chimica Acta
Lisa Tripodi, Daan Witters, Tadej Kokalj, Heinrich J Huber, Robert Puers, Jeroen Lammertyn, Dragana Spasic
Detection methods that do not rely on the amplification of DNA but can reach sensitivity, specificity and throughput of gold standard methods, such as qPCR, have been extensively explored in recent years. Here, we present a hydrophilic-in-hydrophobic (HIH)-microwell array platform that empowers a panel of different amplification-free DNA bioassays: digital enzyme-linked oligonucleotide assay (ELONA), ligation-assisted (LA) digital ELONA and so-called 'analog' bioassays. We developed all three bioassays by using magnetic beads for capturing DNA target, followed by hybridization of enzyme-labelled detection probes and sealing of the built complexes into the femtoliter HIH microwells to achieve the fluorescent readout of single DNA molecules...
December 24, 2018: Analytica Chimica Acta
Keenan Mintz, Eric Waidely, Yiqun Zhou, Zhili Peng, Abdulrahman O Al-Youbi, Abdulaziz S Bashammakh, Mohammad S El-Shahawi, Roger M Leblanc
Hepatocellular carcinoma (HCC) is among the leading causes of mortality in the world. The detection of HCC in its early stage is the key for early treatment and thus the improvement of the chances of survival. Among the various methods of HCC screening, assays based on the detection of biomarker that is specific to HCC such as alpha-l-fucosidase (AFU) have been regarded as the most prominent methods. In this regards, a new assay for the detection of AFU to screen HCC was developed. This assay was based on the energy transfer between carbon dots (C-dots) and gold nanoparticles (AuNPs), the concentration of AFU could be monitored by the degree of C-dots fluorescence quenching due to the energy transfer...
December 24, 2018: Analytica Chimica Acta
Chieh Lin, Yung-Cheng Jair, Yi-Chieh Chou, Pai-Shan Chen, Yi-Chun Yeh
Clinicians require a simple quantitative method for the detection of both phenylalanine and tyrosine to facilitate the diagnosis of phenylketonuria, a common inherited disorder of amino acid metabolism. In this study, we developed a novel whole-cell biosensor for the quantification of phenylalanine and tyrosine through the expression of red and green fluorescent proteins. The proposed system responds specifically and sensitively to phenylalanine/tyrosine without interferences from other amino acids. Furthermore, the precision of the biosensor was evaluated using specimens of normal human urine by LC-MS...
December 24, 2018: Analytica Chimica Acta
Chao Li, Jiehua Ma, Hai Shi, Xiaolu Hu, Yang Xiang, Yuanyang Li, Genxi Li
Advanced methods for developing and applying responsive DNA nanodevices are of great interest. Herein, we report a stretchable DNAzyme that allows simple, multiplexed and sensitive fluorescent detection of antibodies. We find that rigid antibody can tightly stretch soft, antigen-labelled DNAzyme strand and disrupt the hybridization between DNAzyme and its substrate. Based on this finding, we develop a novel strategy to detect antibodies. Due to the robustness and high activity of DNAzyme, this assay can easily detect target as low as 1 ± 0...
December 24, 2018: Analytica Chimica Acta
Pradip Das, Abootaleb Sedighi, Ulrich J Krull
The development of bioanalytical methods that provide early detection of the presence of cancer by sensitive and specific determination of biomarkers such as small biomolecules, nucleic acids, proteins, enzymes, and even whole cells are essential to improve opportunity for improved patient treatment and to diminish the rate of cancer mortality. Förster resonance energy transfer (FRET) methods have been increasingly used to develop bioassays that offer speed, selectivity and low detection levels with practicality that is appropriate for providing point-of-care measurements for screening...
December 24, 2018: Analytica Chimica Acta
Pavel Pidenko, Huiyan Zhang, Pieterjan Lenain, Irina Goryacheva, Sarah De Saeger, Natalia Beloglazova
A strategy to design an immunoassay based on imprinted proteins to detect a foodborne toxin zearalenone (ZEN) has been proposed. Proteins were used as scaffolds for generation of binding cavities with a high specificity against ZEN. Different proteins and different bioimprinting approaches were tested. The imprinted proteins primarily prepared in a tube and then immobilized on a microwell plate, and directly prepared in the microwell plate were compared for an application in immunosorbent detection of ZEN in naturally contaminated wheat and maize samples...
December 21, 2018: Analytica Chimica Acta
Li Gao, Ruihuan Zhao, Yiwen Wang, Mei Lu, Dingding Yang, Mengmei Fa, Xin Yao
Abnormal O-linked-N-acetylglucosamine (O-GlcNAc) concentrations have been associated with a variety of diseases (e.g., cancer, Alzheimer's disease, cardiovascular disease, etc.). However, O-GlcNAc detection is complicated, time-consuming and has poor specificity, therefore, the accurate detection of O-GlcNAc is difficult. In this study, an accurate and sensitive surface plasmon resonance (SPR) biosensor for O-GlcNAc detection that is based on β-D-N-acetylglucosaminidase (OGA) and Au nanoparticles (AuNPs) was developed...
December 21, 2018: Analytica Chimica Acta
Bo Pang, Kaiyue Fu, Yushen Liu, Xiong Ding, Jiumei Hu, Wenshuai Wu, Kun Xu, Xiuling Song, Juan Wang, Ying Mu, Chao Zhao, Juan Li
Foodborne pathogen is the primary cause of foodborne disease outbreak. Given its great damage, a sensitive, simple and rapid detection method is demanded. Herein, we described a self-priming polydimethylsiloxane (PDMS)/paper hybrid microfluidic chip, termed SPH chip, with mixed-dye-loaded loop-mediated isothermal amplification (LAMP) for multiplex foodborne pathogens detection. Staphylococcus aureus (SA) and Vibrio parahaemolyticus (VP) were chosen to verify the novel method. Compared to other similar detection devices, the SPH chip required easier fabrication process, less operation steps and lower cost...
December 21, 2018: Analytica Chimica Acta
Nicolò Dossi, Rosanna Toniolo, Fabio Terzi, Nicola Sdrigotti, Franco Tubaro, Gino Bontempelli
A simple and sensitive device is presented based on the use of pencil-drawn paper based electrochemical detector placed at the end of a cotton thread fluidic channel in wall-jet configuration. This innovative and fast responding electroanalytical system can be adopted for both single and dual electrode electrochemical detection, this last achieved by applying two different potentials at two independent working electrodes drawn on the opposite faces of the paper based detector. Its performance was preliminarily optimized by adopting hexacyanoferrate(II) as probe species undergoing reversible electrochemical processes...
December 21, 2018: Analytica Chimica Acta
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