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Acta Virologica

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https://www.readbyqxmd.com/read/29895165/kaempferol-inhibited-bovine-herpesvirus-1-replication-and-lps-induced-inflammatory-response
#1
L Zhu, P Wang, W Yuan, G Zhu
Plant-derived flavonoids contain large amount of compounds with pharmacological effects. In this study, we showed the compound Kaempferol to have robust antiviral activity against bovine herpesvirus 1 (BoHV-1) replication in vitro. Kaempferol at a concentration of 100 μmol/l completely inhibited viral replication in MDBK cells. It mainly affects the viral replication at the post-entry stages. The inhibition of Akt signaling is a potential mechanism underlying the antiviral effect of Kaempferol. In addition, at a concentration of 25 and 50 μmol/l Kaempferol could significantly reduce the expression of inflammatory mediators such as tumor necrosis factor alpha (TNF-α), interleukin-8 (IL-8) and macrophage inflammatory protein 1 alpha (MIP-1α) in human promonocytic U937 cells-derived macrophages (dU937) in response to lipopolysaccharides (LPS) stimulation...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895164/phylogenetic-relationships-of-two-ukrainian-tomato-isolates-of-potato-virus-m-and-genetic-variability-analysis-of-its-population
#2
L Mishchenko, A Dunich, O Taran, A Dashchenko, V Polischuk, O Kondratyuk
The aim of this study was to investigate biological and molecular properties of two Ukrainian tomato isolates of potato virus M (PVM), K-16 and Pol-14, to determine their phylogenetic relationships and the genetic variability of PVM isolates. Study of phylogenetic relationships of two Ukrainian tomato PVM isolates with 35 isolates represented in GenBank was conducted. It was found that the coat protein (CP) gene sequence identity between two Ukrainian PVM isolates is 94.3% at the nucleotide level and 100% at the amino acid level...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895163/evidence-of-hepatitis-a-virus-infection-in-the-patients-with-acute-encephalitis-syndrome-in-gorakhpur-region-north-india
#3
M S Joshi, B V Tandale, M M Gore, S Bhalla, Y K Gurav, G N Sapkal, K P Kushwaha, A C Mishra, S D Chitambar
The etiological agent remained unidentified in a large number of patients hospitalized for acute encephalitis syndrome (AES) in 2008-2009 in Uttar Pradesh and Bihar, north India. All patients were found to present with fever and altered sensorium, while 28%, 19% and 13% showed hepatomegaly, splenomegaly and meningeal signs, respectively. Involvement mostly of children with abnormal hepatic features prompted us to undertake an exploratory study on viral hepatitis A to determine its association, if any, with hepatic derangements...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895162/two-agroinfection-compatible-fluorescent-protein-tagged-infectious-cdna-clones-of-papaya-leaf-distortion-mosaic-virus-facilitate-the-tracking-of-virus-infection
#4
D C Tuo, P Yan, G Y Zhao, X Y Li, P Zhou, W T Shen
Papaya leaf distortion mosaic virus (PLDMV, the genus Potyvirus) is an emerging threat to papaya production. Here, agroinfection-compatible fluorescent protein-tagged PLDMV infectious cDNA clones driven by the Cauliflower mosaic virus 35S promoter were successfully constructed using one-step Gibson assembly. The clones were directly transformed into Agrobacterium tumefaciens to prevent potential problems such as plasmid instability during propagation in Escherichia coli. Ninety-five percent of papaya seedlings infected with PLDMV-GFP or PLDMV-mCherry developed systemic symptoms typical of those caused by wild-type PLDMV...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895161/prevalence-and-genetic-features-of-hepatitis-e-virus-in-swine-in-gansu-china
#5
J-H Zhou, Y-N Wang, X-R Li, Y-S Liu, Q Pan, X Lan
The prevalence of hepatitis E virus (HEV) in domestic swine threats the public health, due to the risk of zoonotic transmission. In this study, we investigated the prevalence and genetic features of HEV in swine, in Gansu, which is geographically an important province in China. 377 fecal samples were collected from pig farms in Gansu province of China and tested for HEV RNA by RT-PCR. The prevalence rate is about 23% in pig farms of Gansu province. By RT-nPCR, 5' and 3' RACE methods, a whole genome with 7,284 nt in length, termed as swCH189, was obtained and investigated by nucleotide, codon and amino acid usage analyses...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895160/antiviral-effect-of-a-derivative-of-isonicotinic-acid-enisamium-iodide-fav00a-against-influenza-virus
#6
D Cocking, J Cinatl, D A Boltz, X Peng, W Johnson, M Muzzio, O Syarkevych, G Kostyuk, A Goy, L Mueller, V I Margitich
With only a single class of antiviral drugs existing for treatment of influenza (neuraminidase inhibitors), the search for novel effective compounds is urgently needed. We evaluated a low molecular mass compound, enisamium iodide (FAV00A), against influenza virus infections in primary differentiated normal human bronchial epithelial (NHBE) cells, and in ferrets. FAV00A (500 µg/ml) markedly inhibited influenza virus replication and reduced viral M-gene expression in NHBE cells. Treatment of ferrets with FAV00A (200 mg/kg once daily for 7 days) initiated 24 h after inoculation with 105 TCID50 of influenza A/Wisconsin/67/2005 (H3N2) virus resulted in a significant decrease in virus titers in the upper respiratory tract...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895159/in-search-of-effective-h1n1-neuraminidase-inhibitor-by-molecular-docking-antiviral-evaluation-and-membrane-interaction-studies-using-nmr
#7
K Malbari, H Gonsalves, A Chintakrindi, D Gohil, M Joshi, S Kothari, S Srivastava, A Chowdhary, M Kanyalkar
Considering the need for discovery of new antiviral drugs, in view to combat the issue of resistance particularly to anti-influenza drugs, a series of 2'-amino, 3'-amino and 2', 4'-dihydroxy chalcone derivatives were designed. Structure-based drug design was used to design inhibitors of influenza virus - H1N1 neuraminidase enzyme. These were further optimized by a combination of iterative medicinal chemistry principles and molecular docking. Based on the best docking scores, some chalcone derivatives were synthesized and characterized by infrared spectroscopy (IR) and proton nuclear magnetic resonance (NMR)...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895158/characterization-of-a-new-genotype-of-betapapillomavirus-hpv-17-through-l1-e7-e7-and-lcr-sequences
#8
L Oliveira, L Santos, F Neves
Human papillomavirus (HPV) exhibits epithelial and mucosal tropism. HPV type 17 belongs to the Betapapillomavirus genus and molecular cloning experiments have identified two subtypes (17a and 17b) isolated from epidermodysplasia verruciformis (EV). HPV subtypes are characterized by dissimilarities from 2 to 10% at the nucleotide level from their referenced HPV. The aim of this study was to characterize the L1, E6, E7 and LCR sequences from an isolate, which was recovered from the oral mucosa of an asymptomatic 63 year-old woman...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895157/construction-identification-and-immunogenic-assessments-of-an-hsv-1-mutant-vaccine-with-a-ul18-deletion
#9
L Wang, Q Aoli Wang, F Jin, S Fang, F Luo, Y Wu, F Li, J Liu, Y Wang, J Jin, X Liao, Z Ren, Y Wang
HSV-1 is a mucosal and nerve pathogen, whose morbidity shows an increasing tendency. Although several antiviral drugs exist, there is no cure for viral latency for virtually all carriers. There is an urgent need for an HSV-1 vaccine to control infection and limit its spread and recurrence. The UL18 gene, encoding a vital component of capsids, is one of the essential genes of HSV-1. Deletion of UL18 from HSV-1 may be exploited as a new approach to develop an attenuated vaccine. The purpose of this study was to construct a DNA vaccine with a full-length UL18 gene deletion of the HSV-1 genome that can induce an effective immune response...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895156/the-influence-of-therapeutic-vaccine-candidate-against-hbeag-pegfp-n1-c-472-507-ecdcd40l-on-dendritic-cells
#10
J Zheng, H Xue, H Zhang, L Wang, L Zheng, X Wang, X Lin, S Jin, J Wu
Hepatitis B virus (HBV) infection is a major public health problem and immune tolerance is responsible for persistent HBV infection. HBV therapeutic vaccines targeting HBV e antigen (HBeAg) may have an excellent effect in overcoming HBV immune tolerance. Thus, there is urgency for designing therapeutic vaccine candidates that target HBeAg. In this research, we fused the C (472-507) gene sequence of HBV with the extracellular domain of human CD40 ligand sequence and ligated this fused sequence into the pEGFP-N1 vector to construct the recombinant plasmid pEGFP-N1-C (472-507)-ecdCD40L...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895155/usp15-inhibits-hpv16-e6-degradation-and-catalytically-inactive-usp15-has-reduced-inhibitory-activity
#11
Y Yaginuma, M Yoshimoto, A Tokuda
High-risk human papillomaviruses (HPVs) possess transforming activity leading to development of the cancer, including oropharyngeal, anal, penile, vulvar, vaginal, and cervical cancer. The stability of E6 is essential for its complete function as an oncoprotein. Using the yeast two-hybrid system, we identified ubiquitin-specific protease 15 (USP15) as an HPV16 E6-interacting protein. USP15 cleaves polyubiquitin chains of HPV16 E6 and/or ubiquitin precursors. Our results indicate that USP15 could increase the level of HPV16 E6 by inhibiting E6 degradation...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895154/naked-dna-immunization-with-full-length-attachment-gene-of-human-respiratory-syncytial-virus-induces-safe-and-protective-immune-response
#12
M E Hamad, H M Amer, M A Farrag, A H Osman, F N Almajhdi
Development of potent vaccine for human respiratory syncytial virus (HRSV) that confers better protection than natural infection remains a global challenge. Vaccination with naked DNA is considered successful approach for the control of many viral diseases. In this study, the potential of DNA vaccination using full-length attachment gene of HRSV type A Saudi strain cloned in pcDNA3.1+ vector (pcDNA/GA) was evaluated in BALB/c mice. The expression efficiency of pcDNA/GA was first confirmed in HEp-2 cells on RNA and protein levels...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895153/rsv-infection-in-a-macrophage-cell-line-activates-the-non-canonical-nf-%C3%AE%C2%BAb-pathway-and-induces-pro-inflammatory-cytokine-expression
#13
O Del Moral-Hernández, C Santiago-Olivares, E Rivera-Toledo, J Gaona, E Castillo-Villanueva, B Gómez
Respiratory syncytial virus (RSV) is a highly prevalent infectious agent that causes severe respiratory tract illnesses in infants and children worldwide. Children who have suffered severe RSV infections during infancy are prone to develop recurrent episodes of wheezing and asthma that may be associated with viral persistence. RSV infections in humans and animal models are characterized by extensive inflammatory responses. Epithelial cell lines acutely infected by RSV have shown activation of the NF-κB signaling through two independent pathways: the canonical pathway, mediated by RelA and p50 subunits, and the non-canonical pathway, mediated by the subunits RelB and p52...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895152/identification-of-a-novel-plant-amalgavirus-amalgavirus-amalgaviridae-genome-sequence-in-cistus-incanus
#14
C J Goh, D Park, J S Lee, F Sebastiani, Y Hahn
Amalgaviridae is a family of double-stranded, monosegmented RNA viruses that are associated with plants, fungi, microsporidians, and animals. A sequence contig derived from the transcriptome of a eudicot, Cistus incanus (the family Cistaceae; commonly known as hoary rockrose), was identified as the genome sequence of a novel plant RNA virus and named Cistus incanus RNA virus 1 (CiRV1). Sequence comparison and phylogenetic analysis indicated that CiRV1 is a novel species of the genus Amalgavirus in the family Amalgaviridae...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29895151/hepatitis-b-virus-vaccine-breakthrough-infection-surveillance-of-s-gene-mutants-of-hbv
#15
Y Qin, P Liao
Hepatitis B (HB) is a worldwide public health problem, closely related with liver cirrhosis and hepatocellular carcinoma (HCC). The implementation of universal hepatitis B virus (HBV) vaccination programs has led to significant reduction in incidence of acute and chronic HB, liver cirrhosis and HCC. However, this success is now being threatened by the discovery of HBV vaccine breakthrough infection caused by the S gene mutants of HBV, high maternal viral load and virus-induced immunosuppression. An alteration in the antigenicity and immunogenicity of hepatitis B surface antigen (HBsAg) due to S gene mutations may compromise detection of HBsAg (diagnosis-escape mutants), treatment with hepatitis B-specific immunoglobulin (HBIG), and even cause infections in individuals who are antihepatitis B surface antigen (anti-HBs) antibody-positive after immunization (vaccine-escape mutants)...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29521110/in-vitro-micrografting-of-different-prunus-species-by-cherry-adapted-plum-pox-virus-isolate
#16
Z Vozárová, A Nagyová, S Nováková
No abstract text is available yet for this article.
2018: Acta Virologica
https://www.readbyqxmd.com/read/29521109/an-isothermal-based-recombinase-polymerase-amplification-assay-for-rapid-sensitive-and-robust-indexing-of-citrus-yellow-mosaic-virus
#17
P V Kumar, S K Sharma, N Rishi, D K Ghosh, V K Baranwal
Management of viral diseases relies on definite and sensitive detection methods. Citrus yellow mosaic virus (CYMV), a double stranded DNA virus of the genus Badnavirus, causes yellow mosaic disease in citrus plants. CYMV is transmitted through budwood and requires a robust and simplified indexing protocol for budwood certification programme. The present study reports development and standardization of an isothermal based recombinase polymerase amplification (RPA) assay for a sensitive, rapid, easy, and cost-effective method for detection and diagnosis of CYMV...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29521108/a-survey-on-murine-gammaherpesvirus-68-in-ticks-collected-in-slovakia
#18
M Kúdelová, M Slovák, P Kabát, T Derka, B Števove, M Bohuš, M Vrbová
Murine gammaherpesvirus 68 (MHV-68) is a natural pathogen that infects murid rodents which serve as hosts for Dermacentor reticulatus and Ixodes ricinus ticks. For the first time, MHV-68 was detected in immature I. ricinus ticks feeding on lizards trapped in Slovakia. Later on, MHV-68 infection was detected in D. reticulatus and Haemaphysalis concinna ticks collected on vegetation, which supported the idea that ticks can acquire the virus from feeding on infected hosts. Here, we report MHV-68 infection, which was detected by nested PCR, in D...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29521107/sequence-analysis-of-vp2-hypervariable-region-of-the-field-isolates-of-infectious-bursal-disease-viruses-from-southern-region-of-india
#19
P Raja, T M A Senthilkumar, C V Priyadarshini, M Parthiban, A Thangavelu, A Mangala Gowri, A Palanisammi, K Kumanan
Infectious bursal disease virus isolates obtained from southern parts of India were subjected to comparative sequencing and phylogenetic analysis of 743bp hypervariable region of VP2. The sequence analysis showed that among eight isolates, only HY12 showed the characteristic conserved amino acid residues at 256I, 294I, and 299S of vvIBDV. Six isolates BGE14, PY12, NKL14, VCN14, RPM14 and EDE14 had conserved amino acid residues at 256I and 299S, whereas at residue 294, isoleucine was substituted by valine. The remaining isolate MB11 had leucine at residue 294 and asparagine at residue 299 similar to classical strain 52/70...
2018: Acta Virologica
https://www.readbyqxmd.com/read/29521106/wogonin-a-flavonoid-isolated-from-scutellaria-baicalensis-has-anti-viral-activities-against-influenza-infection-via-modulation-of-ampk-pathways
#20
R-K Seong, J-A Kim, O S Shin
Wogonin, a flavonoid isolated from Scutellaria baicalensis, has attracted increasing scientific attention in recent years because of its potent anti-tumor activity. Its role during viral infection has largely been unexplored. Wogonin treatment effectively suppressed both influenza A and B virus replication in Madin-Darby Canine Kidney (MDCK) cells and human lung epithelial (A549) cells. In contrast, wogonin treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling...
2018: Acta Virologica
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