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Acta Histochemica

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https://www.readbyqxmd.com/read/28233575/wnt-1-immunodetection-in-the-regenerating-tail-of-lizard-suggests-it-is-involved-in-the-proliferation-and-distal-growth-of-the-blastema
#1
Lorenzo Alibardi
Lizard tail regeneration depends from growth of the apical tip and autonomous regeneration of a new spinal cord, cartilaginous tube and muscles. The presence of embryonic signaling pathways is likely involved and we have focused on immunolocalization of Wnt1 protein in regenerating tissues, a protein promoting proliferation and tumorigenesis. Western blot indicates some immunoreactive bands in the expected range at 46 and 33kDa in the regenerating tail. Immunolocalization indicates that Wnt1 is prevalently detected in the apical wound epidermis, blastema, and ependyma ampulla of the regenerating tail while it lowers in other tissues of more proximal regions close to the original tail stump...
February 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28233574/comparative-study-of-two-models-of-combined-pulmonary-fibrosis-and-emphysema-in-mice
#2
Wan-Guang Zhang, Si-Si Wu, Li He, Qun Yang, Yi-Kuan Feng, Yue-Tao Chen, Guo-Hua Zhen, Yong-Jian Xu, Zhen-Xiang Zhang, Jian-Ping Zhao, Hui-Lan Zhang
Combined pulmonary fibrosis and emphysema (CPFE) is an "umbrella term" encompassing emphysema and pulmonary fibrosis, but its pathogenesis is not known. We established two models of CPFE in mice using tracheal instillation with bleomycin (BLM) or murine gammaherpesvirus 68 (MHV-68). Experimental mice were divided randomly into four groups: A (normal control, n=6), B (emphysema, n=6), C (emphysema+MHV-68, n=24), D (emphysema+BLM, n=6). Group C was subdivided into four groups: C1 (sacrificed on day 367, 7 days after tracheal instillation of MHV-68); C2 (day 374; 14days); C3 (day 381; 21days); C4 (day 388; 28days)...
February 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28216069/neuronal-differentiation-in-the-early-human-retinogenesis
#3
Anita Rancic, Natalija Filipovic, Josipa Marin Lovric, Snjezana Mardesic, Mirna Saraga-Babic, Katarina Vukojevic
AIM: Our study investigates the differentiation of retinal stem cells towards different neuronal subtypes during the critical period of human eye development. METHODS: Expression of the neuronal marker neurofilament 200 (NF200), tyrosine hydroxilase (TH) and choline acetyltransferase (ChAT) was seen by immunofluorescence in the 5th-12th - week stage of development in the human eye. Data was analysed by Mann-Whitney, Kruskal-Wallis and Dunn's post hoc tests. RESULTS: NF200, TH and ChAT cells appeared in the 5th/6th week and gradually increased during further development...
February 16, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28215504/a-case-of-in-vivo-iontophoresis-assisted-corneal-collagen-cross-linking-for-keratoconus-an-immunohistochemical-study
#4
Mirko Manetti, Eleonora Favuzza, Eleonora Sgambati, Rita Mencucci, Mirca Marini
The standard corneal collagen cross-linking (CXL), that includes the removal of corneal epithelium to permit adequate penetration of riboflavin in the stroma, is an established procedure to halting keratoconus progression. However, as epithelial removal may cause postoperative pain and an increased risk of corneal infection, new therapeutic approaches have been proposed. Iontophoresis is a recently developed non-invasive technique which provides the use of electrical current during CXL to enhance transepithelial penetration of riboflavin into the corneal stroma...
February 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28202179/effects-of-melatonin-in-rats-in-the-initial-third-stage-of-pregnancy-exposed-to-sub-lethal-doses-of-herbicides
#5
Lécio Leone de Almeida, Álvaro Aguiar Coelho Teixeira, Anísio Francisco Soares, Franklin Magliano da Cunha, Valdemiro Amaro da Silva, Leucio Duarte Vieira Filho, Valéria Wanderley-Teixeira
: Exposure to the herbicides Paraquat (PQ) and Roundup(®) may cause cell lesions due to an increase in oxidative stress levels in different biological systems, even in the reproductive system. OBJECTIVE: Evaluate the possible changes in reproductive parameters and hepatic, as well as its prevention by simultaneous application of melatonin. METHODS: Thirty-five female rats at the age of 3 months were divided into seven groups: three groups exposed to sub-lethal doses of the herbicides PQ (50mg/kg) and Roundup(®) (500mg/kg) (n=5, G2, G3 and G4); three groups exposed to herbicides and simultaneous treatment with 10mg/kg of Melatonin (n=5, G5, G6 and G7) and control group (n=5, G1) from the first to the seventh day of pregnancy...
February 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28187905/role-of-rho-specific-guanine-nucleotide-dissociation-inhibitor-%C3%AE-regulation-in-cell-migration
#6
REVIEW
Fei Xie, Shuai Shao, Aziz Ur Rehman Aziz, Baohong Zhang, Hanqin Wang, Bo Liu
Cell migration is a vital process for many physiological and pathological events, and Rho GTPases have been confirmed as key factors in its regulation. The most studied negative regulator of Rho GTPases, Rho-specific guanine nucleotide dissociation inhibitor α (RhoGDIα), mediates cell migration through altering the overall expression and spatiotemporal activation of Rho GTPases. The RhoGDIα-Rho GTPases dissociation can be mediated by signal pathways targeting RhoGDIα directly. This review summarizes the research about the regulation of RhoGDIα during cell migration, which can be in a Rho GTPases association independent manner...
February 7, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174029/effect-of-dietary-zinc-on-morphological-characteristics-and-apoptosis-related-gene-expression-in-the-small-intestine-of-bama-miniature-pigs
#7
Xin Zhou, Yansen Li, Zhaojian Li, Yun Cao, Fei Wang, ChunMei Li
To investigate the effects of dietary zinc (Zn) on small intestinal mucosal epithelium, 6-month-old male Bama miniature pigs were randomly allocated into three groups and treated with three levels of Zn (Control, T1, and T2 diet supplemented with 0, 50, and 1500mg/kg Zn, respectively, as zinc sulfate) for 38days. The samples of small intestine tissues, serum, and feces were collected. The results showed that Zn concentrations of small intestine in the T2 group were higher than those in the control and T1 groups (p<0...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174028/immunoreactivity-pattern-of-calretinin-in-the-developing-human-cerebellar-cortex
#8
Valeria Pibiri, Clara Gerosa, Laura Vinci, Gavino Faa, Rossano Ambu
The immunohistochemical expression of the calcium-binding protein calretinin during human cerebellar development has been investigated in this study. Human cerebellum samples, obtained from 7 fetuses and newborns ranging from 11 to 38 weeks of gestation, were 10% formalin-fixed, routinely processed and paraffin-embedded. 3μm-tick sections were immunostained with an anti-calretinin antibody. Our study evidenced a different immunoreactivity for calretinin in Purkinje cells and in several cerebellar interneurons at different intrauterine developmental stages...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174027/forkhead-box-p3-regulatory-t-cells-and-toll-like-receptor-2-co-expression-in-oral-squamous-cell-carcinoma
#9
H M Hussaini, V P B Parachuru, G J Seymour, A M Rich
BACKGROUND: The function of forkhead box-P3 (FoxP3) regulatory T cells (Treg) and toll-like receptor (TLR)2 protein in the oral cancer microenvironment is not fully understood, but evidence from other malignancies suggests it is likely they are involved with tumour development and progression. The aim of this study was to investigate the distribution of FoxP3(+)cells, TLR2(+) cells and double-labelled FoxP3(+)TLR2(+) immune cells in oral squamous cell carcinoma (OSCC), using immunohistochemistry (IHC) and immunofluorescence (IF)...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168996/ribosome-biogenesis-and-cancer
#10
REVIEW
Massimo Derenzini, Lorenzo Montanaro, Davide Trerè
There is growing evidence indicating that the human pathological conditions characterized by an up-regulated ribosome biogenesis are at an increased risk of cancer onset. At the basis of this relationship is the close interconnection between the ribosome biogenesis and cell proliferation. Cell proliferation-stimulating factors also stimulate ribosome production, while the ribosome biogenesis rate controls the cell cycle progression. The major tumour suppressor, the p53 protein, plays an important balancing role between the ribosome biogenesis rate and the cell progression through the cell cycle phases...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168995/starch-derived-absorbable-polysaccharide-hemostat-enhances-bone-healing-via-bmp-2-protein
#11
Wen Niu, Yanxia Wang, Yi Liu, Bo Zhang, Manling Liu, Ying Luo, Pengtao Zhao, Yao Zhang, Hanrong Wu, Lei Ma, Zhichao Li
Surgical hemostasis is critical in reducing the likelihood of excessive bleeding and blood transfusion. In treating some cases, commonly used hemostatic agent showed limited efficacy and prolonged degradation and clearance, causing an inhibition of bone healing. Starch absorbable polysaccharide (SAPH) is a novel hemostatic agent made from a plant starch, which can be completely absorbed and achieve better hemostatic effects than many commonly used hemostatic agents. However, whether SAPH can induce a promotion of bone healing remains unknown...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168994/oxidative-stress-and-no-generation-in-the-rat-pancreatitis-induced-by-pancreatic-duct-ligation
#12
Igor Buchwalow, Jürgen Schnekenburger, Dmitri Atiakshin, Vera Samoilova, Eduard Wolf, Werner Boecker, Katharina Tiemann
The interaction between nitric oxide (NO) and superoxides is critical in the development of an acute pancreatitis. Previously, we reported that the expression of superoxides and of the NO-generating enzyme (NO synthase, NOS) was up-regulated in the human pancreatitis, especially within the exocrine compartment indicating an exceptional susceptibility of the exocrine parenchyma to oxidative stress. The aim of the present study was to compare the regulation of NO signalling pathways in the human pancreatitis and in an animal model of an acute pancreatitis induced by pancreatic duct ligation (PDL) in rats...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28034458/corrigendum-to-acute-effects-of-stretching-exercise-on-the-soleus-muscle-of-female-aged-rats-acta-histochem-118-1-2016-1-9
#13
Talita Gianello Gnoato Zotz, Luiz Guilherme A Capriglione, Rafael Zotz, Lucia Noronha, Marina Louise Viola De Azevedo, Hilana Rickli Fiuza Martins, Anna Raquel Silveira Gomes
No abstract text is available yet for this article.
December 26, 2016: Acta Histochemica
https://www.readbyqxmd.com/read/28126192/using-vitamin-e-to-prevent-the-impairment-in-behavioral-test-cell-loss-and-dendrite-changes-in-medial-prefrontal-cortex-induced-by-tartrazine-in-rats
#14
Ali Rafati, Nasrin Nourzei, Saied Karbalay-Doust, Ali Noorafshan
Tartrazine is a food color that may adversely affect the nervous system. Vitamin E is a neuro-protective agent. This study aimed to evaluate the effects of tartrazine and vitamin E on the performance of rats in memory and learning tests as well as the structure of medial Prefrontal Cortex (mPFC). The rats were first divided into seven groups which received the followings for a period of seven weeks: distilled water, corn oil, vitamin E (100mg/kg/day), a low dose (50mg/kg/day) and a high dose (50mg/kg/day) of tartrazine with and without vitamin E...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28122663/the-possible-protective-role-of-pumpkin-seed-oil-in-an-animal-model-of-acid-aspiration-pneumonia-light-and-electron-microscopic-study
#15
Nesreen Moustafa Omar, Nahla Reda Sarhan
Aspiration pneumonitis is a common problem occurring in many clinical disorders. Pumpkin seed oil (PO) is a rich source of antioxidants. This work aimed to assess the effect of PO on the lung histopathological changes induced by acid aspiration. Forty male albino rats assigned to four groups were used. Rats of control group were instilled intratracheally with normal saline 2mL/kg. HCL group instilled with 2mL/kg of HCL 0.1N, pH 1.25. PO group received pumpkin seed oil (PO) orally (∼1375mg/kgbw/day) for 7days...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28110937/adult-human-retinal-m%C3%A3-ller-glia-display-distinct-peripheral-and-macular-expression-of-cd117-and-cd44-stem-cell-associated-proteins
#16
Lay Khoon Too, Gary Gracie, Enisa Hasic, Julia H Iwakura, Svetlana Cherepanoff
Experimental evidence suggests human Müller glia exhibit neural progenitor properties in vitro. CD117 and CD44 are known to be expressed by stem cells, the survival of which appears to depend critically on interactions with hyaluronan-rich extracellular matrix (ECM). Here, we characterise Müller glia expression of CD117 and CD44 in normal adult human retina and describe how it correlates with hyaluronan distribution in ocular ECM. By using chromogen-based immunohistochemistry, CD117 expression was found in entire Müller glia cytoplasm spanning from inner to outer limiting membrane in both peripheral retina (PR) and macular retina (MR), mirroring expression of the established Müller glia marker vimentin...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28063600/rna-in-situ-hybridization-characterization-of-non-enzymatic-derived-bovine-intervertebral-disc-cell-lineages-suggests-progenitor-cell-potential
#17
Petra Kraus, Rachel Yerden, Victoria Kocsis, Thomas Lufkin
Degeneration of the intervertebral disc (IVD) is a meritorious target for therapeutic cell based regenerative medicine approaches, however, controversy over what defines the precise identity of mature IVD cells and lack of single cell based quality control measures is of concern. Bos taurus and human IVDs are histologically more similar than is Mus musculus. The mature bovine IVD is well suited as model system for technology development to be translated into therapeutic cell based regenerative medicine applications...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28017359/mucin-secreting-cells-in-the-stomach-and-colon-are-altered-by-combination-antiretroviral-treatment-in-an-obese-rat-model
#18
Danélle Truter, Hans Strijdom, Frans Everson, Sanet H Kotzé
Mucins, secreted by intestinal goblet cells, form an integral part of the intestinal biofilm, which is important for the functioning of a healthy gastrointestinal tract (GIT). This mucous layer is sensitive to factors such as diet, drugs and inflammation. Histochemically, mucins can be classified as neutral or acidic, where acidic mucins can contain sulphate groups (sulphomucins) or sialic acid (sialomucins). The aim of the present study was to determine the composition of various mucin secreting cells using histochemical stains in rats fed on a high calorie diet (HCD) treated with antiretroviral therapy (ART)...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28017358/differentiation-of-mesenchymal-stem-cells-from-human-amniotic-fluid-to-vascular-endothelial-cells
#19
Waleephan Tancharoen, Sirinda Aungsuchawan, Peraphan Pothacharoen, Runchana Markmee, Suteera Narakornsak, Junjira Kieodee, Nonglak Boonma, Witoon Tasuya
Endothelial dysfunction is a principle feature of vascular-related disease. Endothelial cells have been acquired for the purposes of the restoration of damaged tissue in therapeutic angiogenesis. However, their use is limited by expansion capacity and the small amount of cells that are obtained. Human amniotic fluid mesenchymal stem cells (hAF-MSCs) are considered an important source for vascular tissue engineering. In this study, hAF-MSCs were characterized and then induced in order to differentiate into the endothelial-like cells...
March 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28012573/fgf2-fgf3-and-fgf4-expression-pattern-during-molars-odontogenesis-in-didelphis-albiventris
#20
Íria Gabriela Dias Dos Santos, Erika Cristina Jorge, Aline Gonçalves Lio Copola, Bruno Machado Bertassoli, Alfredo Miranda de Goes, Gerluza Aparecida Borges Silva
Odontogenesis is guided by a complex signaling cascade in which several molecules, including FGF2-4, ensure all dental groups development and specificity. Most of the data on odontogenesis derives from rodents, which does not have all dental groups. Didelphis albiventris is an opossum with the closest dentition to humans, and the main odontogenesis stages occur when the newborns are in the pouch. In this study, D. albiventris postnatals were used to characterize the main stages of their molars development; and also to establish FGF2, FGF3 and FGF4 expression pattern...
March 2017: Acta Histochemica
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