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Acta Histochemica

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https://www.readbyqxmd.com/read/28314612/a-simple-imagej-macro-tool-for-analyzing-mitochondrial-network-morphology-in-mammalian-cell-culture
#1
Andrew J Valente, Lucas A Maddalena, Ellen L Robb, Fereshteh Moradi, Jeffrey A Stuart
Mitochondria exist in a dynamic cycle of fusion and fission whose balance directly influences the morphology of the 'mitochondrial network', a term that encompasses the branched, reticular structure of fused mitochondria as well as the separate, punctate individual organelles within a eukaryotic cell. Over the past decade, the significance of the mitochondrial network has been increasingly appreciated, motivating the development of various approaches to analyze it. Here, we describe the Mitochondrial Network Analysis (MiNA) toolset, a relatively simple pair of macros making use of existing ImageJ plug-ins, allowing for semi-automated analysis of mitochondrial networks in cultured mammalian cells...
March 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28291543/cell-membrane-integrity-and-revascularization-the-possible-functional-mechanism-of-ischemic-preconditioning-for-skeletal-muscle-protection-against-ischemic-reperfusion-injury
#2
Yang Hong, Bin Zhang, Ling Yu, Shan-Shan Duan
BACKGROUND: The purpose of this paper was to evaluate whether ischemic preconditioning (IPC) could make protective effects against skeletal muscle injuries induced by ischemic-reperfusion (I/R). METHODS: Eighteen rats were randomly divided into three groups of 6 subjects each: control group, I/R group, and IPC group. Thigh root ischemia of rats in the I/R group was induced by 3h ischemia and 24h reperfusion. IPC was applied by 3 periods of 15min ischemia/15min reperfusion prior to ischemia...
March 10, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28279507/germ-cell-apoptosis-and-expression-of-bcl-2-and-bax-in-porcine-testis-under-normal-and-heat-stress-conditions
#3
Xiaorui Fan, Huaming Xi, Zhen Zhang, Yajun Liang, Qinghong Li, Junping He
The aim of this study was to examine whether an elevated ambient temperature (37-40°C) had an effect on the apoptosis of germ cells and the expression of Bcl-2 and Bax in porcine testis. Six boars were used. Three boars were subjected to an elevated ambient temperature (37-40°C, 7days, 3h per day) as a heat stress (HS) group. The other 3 boars were kept in a room temperature house (20-27°C) as a control group. All boars were castrated and the testes were harvested. TUNEL assay was used for the detection of apoptotic cells...
March 6, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28262328/citrus-flavanones-mildly-interfere-with-pituitary-thyroid-axis-in-old-aged-male-rats
#4
Marko Miler, Ivana Jarić, Jasmina Živanović, Vladimir Ajdžanović, Nasta Tanić, Verica Milošević, Branka Šošić-Jurjević
Citrus flavanones naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving cardiovascular and metabolic status. However, they may also affect thyroid hormone economy. Keeping in mind impaired thyroid function at older age, in this study we tested wheather NAR or HES administration potentiate this decline. NAR or HES were administrated orally (15mg/kg) to male 24-month-old Wistar rats during 4 weeks. Control groups received vehicle, sunflower oil...
March 2, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28262327/immunohistochemical-response-in-rats-of-beta-tricalcium-phosphate-tcp-with-or-without-bmp-2-in-the-production-of-collagen-matrix-critical-defects
#5
Eloá Rodrigues Luvizuto, Júlio César Silva de Oliveira, Pedro Henrique Silva Gomes-Ferreira, Cassiano Costa Silva Pereira, Leonardo Perez Faverani, Cristina Antoniali, Roberta Okamoto
This study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats' calvaria. A critical-size defect (5mm in diameter) was filled with β-TCP alone or added with that plus 5mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing...
March 2, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28238410/histological-and-immunohistochemical-characterization-of-the-mongolian-gerbil-s-mammary-gland-during-gestation-lactation-and-involution
#6
Ellen C R Leonel, Luiz R Falleiros, Silvana G P Campos, Sebastião R Taboga
The morphological description of normal tissues is fundamental for making comparisons and in order to identify injuries and lesions. The aim of this work was to describe the morphological characteristics of the female Mongolian gerbil's (Meriones unguiculatus) normal mammary gland, the average expression of hormone receptors, and the average proliferation rates in the epithelial cells during the periods of lactation, pregnancy and involution. Dams were euthanized on the 14th and 21st gestational days, 7 and 14days after parturition, and 3 and 5days after weaning...
February 23, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28236448/gradual-decrease-in-spermatogenesis-caused-by-chronic-stress
#7
Lizbeth Juárez-Rojas, Rosa María Vigueras-Villaseñor, Fahiel Casillas, Socorro Retana-Márquez
Chronic stress induces decreased sperm motility, viability and concentration in stressed males. Also, stress modifies oxidative status and causes apoptosis in testes, as well as a decrease in the epithelial area of seminiferous tubules. However, there are no studies that analyze the alterations caused by stress in testicular cells. Thus, in this study, alterations in the morphology of testicular germ cells caused by different days of chronic stress were assessed. Adult male rats were exposed to stress by immersion in cold water (ICW) daily for 3, 8, 20 or 50 consecutive days...
February 21, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28233575/wnt-1-immunodetection-in-the-regenerating-tail-of-lizard-suggests-it-is-involved-in-the-proliferation-and-distal-growth-of-the-blastema
#8
Lorenzo Alibardi
Lizard tail regeneration depends from growth of the apical tip and autonomous regeneration of a new spinal cord, cartilaginous tube and muscles. The presence of embryonic signaling pathways is likely involved and we have focused on immunolocalization of Wnt1 protein in regenerating tissues, a protein promoting proliferation and tumorigenesis. Western blot indicates some immunoreactive bands in the expected range at 46 and 33kDa in the regenerating tail. Immunolocalization indicates that Wnt1 is prevalently detected in the apical wound epidermis, blastema, and ependyma ampulla of the regenerating tail while it lowers in other tissues of more proximal regions close to the original tail stump...
February 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28233574/comparative-study-of-two-models-of-combined-pulmonary-fibrosis-and-emphysema-in-mice
#9
Wan-Guang Zhang, Si-Si Wu, Li He, Qun Yang, Yi-Kuan Feng, Yue-Tao Chen, Guo-Hua Zhen, Yong-Jian Xu, Zhen-Xiang Zhang, Jian-Ping Zhao, Hui-Lan Zhang
Combined pulmonary fibrosis and emphysema (CPFE) is an "umbrella term" encompassing emphysema and pulmonary fibrosis, but its pathogenesis is not known. We established two models of CPFE in mice using tracheal instillation with bleomycin (BLM) or murine gammaherpesvirus 68 (MHV-68). Experimental mice were divided randomly into four groups: A (normal control, n=6), B (emphysema, n=6), C (emphysema+MHV-68, n=24), D (emphysema+BLM, n=6). Group C was subdivided into four groups: C1 (sacrificed on day 367, 7 days after tracheal instillation of MHV-68); C2 (day 374; 14days); C3 (day 381; 21days); C4 (day 388; 28days)...
February 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28216069/neuronal-differentiation-in-the-early-human-retinogenesis
#10
Anita Rancic, Natalija Filipovic, Josipa Marin Lovric, Snjezana Mardesic, Mirna Saraga-Babic, Katarina Vukojevic
AIM: Our study investigates the differentiation of retinal stem cells towards different neuronal subtypes during the critical period of human eye development. METHODS: Expression of the neuronal marker neurofilament 200 (NF200), tyrosine hydroxilase (TH) and choline acetyltransferase (ChAT) was seen by immunofluorescence in the 5th-12th - week stage of development in the human eye. Data was analysed by Mann-Whitney, Kruskal-Wallis and Dunn's post hoc tests. RESULTS: NF200, TH and ChAT cells appeared in the 5th/6th week and gradually increased during further development...
February 16, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28215504/a-case-of-in-vivo-iontophoresis-assisted-corneal-collagen-cross-linking-for-keratoconus-an-immunohistochemical-study
#11
Mirko Manetti, Eleonora Favuzza, Eleonora Sgambati, Rita Mencucci, Mirca Marini
The standard corneal collagen cross-linking (CXL), that includes the removal of corneal epithelium to permit adequate penetration of riboflavin in the stroma, is an established procedure to halting keratoconus progression. However, as epithelial removal may cause postoperative pain and an increased risk of corneal infection, new therapeutic approaches have been proposed. Iontophoresis is a recently developed non-invasive technique which provides the use of electrical current during CXL to enhance transepithelial penetration of riboflavin into the corneal stroma...
February 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28202179/effects-of-melatonin-in-rats-in-the-initial-third-stage-of-pregnancy-exposed-to-sub-lethal-doses-of-herbicides
#12
Lécio Leone de Almeida, Álvaro Aguiar Coelho Teixeira, Anísio Francisco Soares, Franklin Magliano da Cunha, Valdemiro Amaro da Silva, Leucio Duarte Vieira Filho, Valéria Wanderley-Teixeira
Exposure to the herbicides Paraquat (PQ) and Roundup(®) may cause cell lesions due to an increase in oxidative stress levels in different biological systems, even in the reproductive system. OBJECTIVE: Evaluate the possible changes in reproductive parameters and hepatic, as well as its prevention by simultaneous application of melatonin. METHODS: Thirty-five female rats at the age of 3 months were divided into seven groups: three groups exposed to sub-lethal doses of the herbicides PQ (50mg/kg) and Roundup(®) (500mg/kg) (n=5, G2, G3 and G4); three groups exposed to herbicides and simultaneous treatment with 10mg/kg of Melatonin (n=5, G5, G6 and G7) and control group (n=5, G1) from the first to the seventh day of pregnancy...
February 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28187905/role-of-rho-specific-guanine-nucleotide-dissociation-inhibitor-%C3%AE-regulation-in-cell-migration
#13
REVIEW
Fei Xie, Shuai Shao, Aziz Ur Rehman Aziz, Baohong Zhang, Hanqin Wang, Bo Liu
Cell migration is a vital process for many physiological and pathological events, and Rho GTPases have been confirmed as key factors in its regulation. The most studied negative regulator of Rho GTPases, Rho-specific guanine nucleotide dissociation inhibitor α (RhoGDIα), mediates cell migration through altering the overall expression and spatiotemporal activation of Rho GTPases. The RhoGDIα-Rho GTPases dissociation can be mediated by signal pathways targeting RhoGDIα directly. This review summarizes the research about the regulation of RhoGDIα during cell migration, which can be in a Rho GTPases association independent manner...
February 7, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174029/effect-of-dietary-zinc-on-morphological-characteristics-and-apoptosis-related-gene-expression-in-the-small-intestine-of-bama-miniature-pigs
#14
Xin Zhou, Yansen Li, Zhaojian Li, Yun Cao, Fei Wang, ChunMei Li
To investigate the effects of dietary zinc (Zn) on small intestinal mucosal epithelium, 6-month-old male Bama miniature pigs were randomly allocated into three groups and treated with three levels of Zn (Control, T1, and T2 diet supplemented with 0, 50, and 1500mg/kg Zn, respectively, as zinc sulfate) for 38days. The samples of small intestine tissues, serum, and feces were collected. The results showed that Zn concentrations of small intestine in the T2 group were higher than those in the control and T1 groups (p<0...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174028/immunoreactivity-pattern-of-calretinin-in-the-developing-human-cerebellar-cortex
#15
Valeria Pibiri, Clara Gerosa, Laura Vinci, Gavino Faa, Rossano Ambu
The immunohistochemical expression of the calcium-binding protein calretinin during human cerebellar development has been investigated in this study. Human cerebellum samples, obtained from 7 fetuses and newborns ranging from 11 to 38 weeks of gestation, were 10% formalin-fixed, routinely processed and paraffin-embedded. 3μm-tick sections were immunostained with an anti-calretinin antibody. Our study evidenced a different immunoreactivity for calretinin in Purkinje cells and in several cerebellar interneurons at different intrauterine developmental stages...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28174027/forkhead-box-p3-regulatory-t-cells-and-toll-like-receptor-2-co-expression-in-oral-squamous-cell-carcinoma
#16
H M Hussaini, V P B Parachuru, G J Seymour, A M Rich
BACKGROUND: The function of forkhead box-P3 (FoxP3) regulatory T cells (Treg) and toll-like receptor (TLR)2 protein in the oral cancer microenvironment is not fully understood, but evidence from other malignancies suggests it is likely they are involved with tumour development and progression. The aim of this study was to investigate the distribution of FoxP3(+)cells, TLR2(+) cells and double-labelled FoxP3(+)TLR2(+) immune cells in oral squamous cell carcinoma (OSCC), using immunohistochemistry (IHC) and immunofluorescence (IF)...
February 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168996/ribosome-biogenesis-and-cancer
#17
REVIEW
Massimo Derenzini, Lorenzo Montanaro, Davide Trerè
There is growing evidence indicating that the human pathological conditions characterized by an up-regulated ribosome biogenesis are at an increased risk of cancer onset. At the basis of this relationship is the close interconnection between the ribosome biogenesis and cell proliferation. Cell proliferation-stimulating factors also stimulate ribosome production, while the ribosome biogenesis rate controls the cell cycle progression. The major tumour suppressor, the p53 protein, plays an important balancing role between the ribosome biogenesis rate and the cell progression through the cell cycle phases...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168995/starch-derived-absorbable-polysaccharide-hemostat-enhances-bone-healing-via-bmp-2-protein
#18
Wen Niu, Yanxia Wang, Yi Liu, Bo Zhang, Manling Liu, Ying Luo, Pengtao Zhao, Yao Zhang, Hanrong Wu, Lei Ma, Zhichao Li
Surgical hemostasis is critical in reducing the likelihood of excessive bleeding and blood transfusion. In treating some cases, commonly used hemostatic agent showed limited efficacy and prolonged degradation and clearance, causing an inhibition of bone healing. Starch absorbable polysaccharide (SAPH) is a novel hemostatic agent made from a plant starch, which can be completely absorbed and achieve better hemostatic effects than many commonly used hemostatic agents. However, whether SAPH can induce a promotion of bone healing remains unknown...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28168994/oxidative-stress-and-no-generation-in-the-rat-pancreatitis-induced-by-pancreatic-duct-ligation
#19
Igor Buchwalow, Jürgen Schnekenburger, Dmitri Atiakshin, Vera Samoilova, Eduard Wolf, Werner Boecker, Katharina Tiemann
The interaction between nitric oxide (NO) and superoxides is critical in the development of an acute pancreatitis. Previously, we reported that the expression of superoxides and of the NO-generating enzyme (NO synthase, NOS) was up-regulated in the human pancreatitis, especially within the exocrine compartment indicating an exceptional susceptibility of the exocrine parenchyma to oxidative stress. The aim of the present study was to compare the regulation of NO signalling pathways in the human pancreatitis and in an animal model of an acute pancreatitis induced by pancreatic duct ligation (PDL) in rats...
February 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28034458/corrigendum-to-acute-effects-of-stretching-exercise-on-the-soleus-muscle-of-female-aged-rats-acta-histochem-118-1-2016-1-9
#20
Talita Gianello Gnoato Zotz, Luiz Guilherme A Capriglione, Rafael Zotz, Lucia Noronha, Marina Louise Viola De Azevedo, Hilana Rickli Fiuza Martins, Anna Raquel Silveira Gomes
No abstract text is available yet for this article.
December 26, 2016: Acta Histochemica
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