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Acta Histochemica

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https://www.readbyqxmd.com/read/28515008/localization-and-distribution-of-gonadal-proteins-in-the-oviparous-lizard-sceloporus-aeneus-squamata-phrynosomatidae
#1
Antonio-Rubio Nivia Rocio, Villagrán-SantaCruz Maricela, Moreno-Mendoza Norma
Among vertebrates, several specific proteins are involved in the function and development of gonads. Several genes such as SOX9, FOXL2, DDX4, IFITM3, and DPPA3, are active during embryonic differentiation and maintain their expression in adult tissues, playing important roles in the function and development of the line cell, where these are produced. Among reptiles, molecular mechanisms for sex differentiation have been analyzed in turtles, crocodiles, and some lizards, while in adult stages such studies are scarce...
May 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28506466/impaired-steroidogenesis-in-the-testis-of-leptin-deficient-mice-ob-ob
#2
Fabiane Ferreira Martins, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda
The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids...
May 13, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28506467/apoptosis-of-bovine-granulosa-cells-intracellular-pathways-and-differentiation
#3
M C Carou, P R Cruzans, A Maruri, M G Farina, C D Fiorito, G Olea, D M Lombardo
Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed an in vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures from ovaries with or without corpus luteum (CPGB+ and CPGB-, respectively), with different doses of gonadotropin releasing hormone (GnRH) analogs (leuprolide acetate (LA) as agonist and antide as antagonist). BGC-1 represent immature granulosa cells, whereas CPGB represent different degrees of luteinization...
May 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28501138/submucosal-neurons-and-enteric-glial-cells-expressing-the-p2x7-receptor-in-rat-experimental-colitis
#4
Marcos Vinícius da Silva, Aline Rosa Marosti, Cristina Eusébio Mendes, Kelly Palombit, Patricia Castelucci
The aim of this study was to evaluate the effect of ulcerative colitis on the submucosal neurons and glial cells of the submucosal ganglia of rats. 2,4,6-Trinitrobenzene sulfonic acid (TNBS; colitis group) was administered in the colon to induce ulcerative colitis, and distal colons were collected after 24h. The colitis rats were compared with those in the sham and control groups. Double labelling of the P2X7 receptor with calbindin (marker for intrinsic primary afferent neurons, IPANs, submucosal plexus), calretinin (marker for secretory and vasodilator neurons of the submucosal plexus), HuC/D and S100β was performed in the submucosal plexus...
May 10, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28499502/sesamin-encouraging-effects-on-chondrogenic-differentiation-of-human-amniotic-fluid-derived-mesenchymal-stem-cells
#5
Suteera Narakornsak, Sirinda Aungsuchawan, Peeraphan Pothacharoen, Runchana Markmee, Waleephan Tancharoen, Tanongsak Laowanitwattana, Chawapon Thaojamnong, Lamaiporn Peerapapong, Nonglak Boonma, Witoon Tasuya, Junjira Keawdee, Naree Poovachiranon
Worldwide, the most recognized musculoskeletal degenerative disease is osteoarthritis (OA). Sesamin, a major abundant lignan compound present in Sesamun Indicum Linn, has been described for its various pharmacological effects and health benefits. However, the promoting effects of sesamin on chondrogenic differentiation have not yet been observed. Herein, the aim of this study was to investigate the effects of sesamin on cell cytotoxicity and the potent supporting effects on chondrogenic differentiation of human amniotic fluid-derived mesenchymal stem cells (hAF-MSCs)...
May 9, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28499501/potential-involvement-of-chondroitin-sulfate-a-in-the-pathogenesis-of-ameloblastoma
#6
Xiangjun Li, Hiroshi Kurita, Tiepeng Xiao, Kyou Iijima, Kenji Kurashina, Jun Nakayama
Ameloblastoma is classified as a benign odontogenic tumor characterized by locally invasive behavior and high risk of recurrence. Here, we evaluate a potential role for glycosaminoglycan, a structural component of cell membranes and extracellular matrix, in ameloblstoma pathogenesis. We subjected formalin-fixed, paraffin-embedded tissue sections of 34 cases of ameloblastoma, 10 of odontogenic keratocyst, and 17 of dentigerous cyst to immunohistochemistry using monoclonal antibodies recognizing chondroitin sulfate A (CS-A), heparan sulfate (HS), and keratan sulfate (KS)...
May 9, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28495367/in-vivo-effects-of-allium-cepa-l-on-the-selected-gut-microflora-and-intestinal-histomorphology-in-broiler
#7
Sajid Ur Rahman, Sarzamin Khan, Naila Chand, Umar Sadique, Rifat Ullah Khan
The experiment was conducted to evaluate the effect of onion (Allium cepa L.,) on the performance, gut microflora and intestinal histomorphology of broiler chicks. Three hundred and twenty day old broiler chicks (Ross) were randomly divided into four groups as A, B, C and D. Group A served as control without any supplementation, group B, C and D was supplemented with onion powder at the rate of 1.5, 2 and 2.5g/kg of feed respectively. Supplementation of onion significantly (P<0.05) increased weight gain and feed consumption...
May 8, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28483062/immunocytochemical-localization-of-cholinergic-amacrine-cells-in-the-bat-retina
#8
Eun-Bee Park, Ya-Nan Gu, Chang-Jin Jeon
The purpose of this study was to localize the cholinergic amacrine cells, one of the key elements of a functional retina, in the retina of a microbat, Rhinolophus ferrumequinum. The presence and localization of choline acetyltransferase-immunoreactive (ChAT-IR) cells in the microbat retina were investigated using immunocytochemistry, confocal microscopy, and quantitative analysis. These ChAT-IR cells were present in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL), as previously reported in various animals...
May 5, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28478955/spatial-distribution-of-cannabinoid-receptor-1-and-fatty-acid-amide-hydrolase-in-the-cat-ovary-and-oviduct
#9
Andrea Pirone, Carla Lenzi, Angela Briganti, Francesco Abbate, Maria Levanti, Francesca Abramo, Vincenzo Miragliotta
Involvement of the endocannabinoid system in female reproduction has been extensively described in humans with the cognate receptors and ligands being found in the ovaries and genital tract. In human, an imbalance of the endocannabinoid system is linked with both ectopic pregnancy and infertility. In bovine species anandamide levels regulate aspects of sperm-oviduct interaction. Here we report the immunohistochemical distribution of cannabinoid receptor 1 (CB1R) and fatty acid amide hydrolase (FAAH) in cat ovary and oviduct, using paraffin-embedded tissue samples and commercially available antibodies...
May 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28478954/acute-infection-with-an-avirulent-strain-of-toxoplasma-gondii-causes-decreasing-and-atrophy-of-nitrergic-myenteric-neurons-of-rats
#10
Débora de Mello Gonçales Sant'Ana, Marcelo Biondaro Gois, Catchia Hermes-Uliana, Letícia Sarturi Pereira-Severi, Emily Martins Baptista, Luana Colognese Mantovani, Aristeu Vieira da Silva, Eduardo José de Almeida Araújo
In the enteric nervous system (ENS), nitrergic neurons produce and use nitric oxide (NO) as an inhibitory motor neurotransmitter in response to parasitic infections, including those caused by Toxoplasma gondii. However, damage to the host caused by NO has been reported by various authors, and the role of NO in protection or cytotoxicity continues to be extensively studied. In this study, nitrergic neurons were investigated in the myenteric plexus of the jejunum and the distal colon of rats infected with 500 oocysts of the M7741 strain of T...
May 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28461019/polar-and-apolar-extra-virgin-olive-oil-and-leaf-extracts-as-a-promising-anti-inflammatory-natural-treatment-for-osteoarthritis
#11
Houda Nsir, Marta Anna Szychlinska, Venera Cardile, Adriana Carol Eleonora Graziano, Rosanna Avola, Hanen Esafi, Alessandra Bendini, Mokhtar Zarouk, Carla Loreto, Venerando Rapisarda, Paola Castrogiovanni, Giuseppe Musumeci
Osteoarthritis (OA) is a common joint disease with important socio-economic impact. Looking for a novel natural treatment represents an important challenge and a public health objective. A wide range of previous studies highlighted the antioxidant and anti-inflammatory properties especially of phenolic compounds present in olive oil. Therefore, the aim of this study was to investigate the anti-inflammatory activity of Tunisian Extra Virgin Olive Oil (EVOO) extracts in an in vitro OA model. To this aim we evaluated the polar and apolar olive oil fractions and olive leaf extract...
April 28, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28457660/ilk-regulates-mscs-survival-and-angiogenesis-partially-through-akt-and-mtor-signaling-pathways
#12
Bin Zeng, Lei Liu, Shaofeng Wang, Zhiguo Dai
Mesenchymal stem cells (MSCs) exert therapeutic effects on treating acute myocardial infarction (AMI). Angiogenesis in ischemic heart can promote the supply of oxygen and nutrients to both ischemic myocardium and transplanted stem cells. Focus is then given to the evolving strategies amied at angiogenesis. ILK has been reported to be an important factor regulating apoptosis and angiogenesis. This study examined the role and mechanism of ILK in MSCs survival and angiogenesis. In hypoxic condition, upregulation of ILK expression increased the phosphorylation of Akt and mTOR, resulting in markedly enchanced MSCs survival and VEGF expression; while significantly inhibited MSCs survival and VEGF expression was detected in MSCs with ILK kinase inactivation, which was associated with a reduction of phosphorylation of Akt and mTOR...
April 28, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28434671/resveratrol-improves-mitochondrial-function-in-the-remnant-kidney-from-5-6-nephrectomized-rats
#13
Yan Hui, Miaomiao Lu, Yarong Han, Hongli Zhou, Wei Liu, Lijing Li, Ruixia Jin
Mitochondrial dysfunction is involved in the pathogenesis of chronic kidney disease (CKD). Resveratrol has been demonstrated to be beneficial for the recovery of kidney diseases. In this study, the 5/6 nephrectomized rat was used as a CKD model and the TGF-β1-exposed mouse mesangial cells were used as an in vitro model. Pathological examination showed that resveratrol treatment attenuated glomerular injury in the remnant kidney of 5/6 nephrectomized rat. Additionally, resveratrol improved mitochondrial function in vivo and in vitro, as evidenced by increasing mitochondrial membrane potential, increasing ATP, decreasing reactive oxygen species production and enhancing activities of complex I and III...
April 20, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28427772/seasonal-variations-of-aromatase-and-estrogen-receptors-expression-in-the-testis-of-free-ranging-sand-rats
#14
Rafik Menad, Souaâd Smaï, Xavier Bonnet, Thérèse Gernigon-Spychalowicz, Elara Moudilou, Farida Khammar, Jean-Marie Exbrayat
An increasing number of studies revealed the importance of estrogen in male reproduction. However, most research was conducted in laboratory rodents subjected to standardized environmental conditions. Therefore, seasonal regulations of estrogen pathways remain poorly understood under natural conditions. Using immunohistochemistry, the expression of several molecules involved in the functioning of testis (i.e. 17-β estradiol [E2], P450 aromatase, estrogen receptors ESR1, ESR2, and GPER1 [also known as GPR30]) were investigated in free-ranging fat sand rats, Psammomys obesus, during the breeding and resting seasons...
April 17, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28389020/seeds-in-the-liver
#15
REVIEW
Hongjie Ji, Yanrong Lu, Yujun Shi
The liver is a crucial organ for homeostasis and has a tremendous self-renewal and regenerative capacity. It has long been believed that the self-renewal and repair of the liver within a given physiological condition or its repopulation in chronic liver diseases, when hepatocyte proliferation is impaired, will primarily be conducted by the proliferating duct cells, termed "oval cells" or hepatic progenitor cells (HPCs). In addition, numerous studies have revealed that HPCs are the initial tumor cells of liver cancer under certain micro-environments...
April 4, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28385400/nanomicelle-curcumin-induced-dna-fragmentation-in-testicular-tissue-correlation-between-mitochondria-dependent-apoptosis-and-failed-pcna-related-hemostasis
#16
Sana Moshari, Vahid Nejati, Gholamreza Najafi, Mazdak Razi
Current study was done to assess possible anti-proliferative effect of nanomicelle curcumin (NMCM) against germ cells in testicular tissue. For this purpose, 24 mature male Wistar rats were divided into control and test groups. The animals in test groups received 7.5mg/kg, 15mg/kg and 30mg/kg of NMC (NO=6 rats in each group). Following 48days, the expression of Bcl-2, Bax, caspase-3, P53 and proliferating cell nuclear antigen (PCNA) were evaluated by using reverse transcription-PCR and immunohistochemistry...
April 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28372808/differential-effect-of-chronic-undernutrition-on-the-fiber-type-composition-of-fascicles-in-the-extensor-digitorum-longus-muscles-of-the-rat
#17
Enrique Vázquez-Mendoza, Erika Elizabeth Rodríguez-Torres, Kenia López-García, Cindy Xilonen Hinojosa-Rodríguez, Ismael Jiménez-Estrada
Several studies have shown that chronic low food consumption alters the composition and metabolism of the extensor digitorum longus muscle (EDLm) fiber types. EDLm is constituted by four independent fascicles (F2-F5) of different sizes; their constitution and metabolism, however, as well as how chronic undernourishment affects these is virtually unknown. Thus, the aim of this study is to evaluate the relative fiber type composition and metabolism of each independent fascicle in the EDLm, using control and chronically undernourished young male rats by using the alkaline ATPase and NADH-TR histochemical techniques...
March 31, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28366506/pulmonary-apoptotic-and-oxidative-damaging-effects-of-triclosan-alone-or-in-combination-with-fluoride-in-sprague-dawley-rats
#18
Amany Tharwat Mohammed, Amany Abdel-Rahman Mohamed, Haytham Ali
This study aimed to assess the potential apoptotic and oxidative damaging impacts of Triclosan (TCS) and Sodium Fluoride (NaF) administered separately or in combination, in rats for thirty days. For this purpose, forty immature female Sprague-Dawley rats were equally allocated into five groups. TCS group administered 185mg TCS/kg Bw dissolved in distilled water (DW), while its control group received only DW. NaF group was given 50mg/kg NaF in corn oil BW and its respective control had corn oil alone. The co-treated group was administered TCS and NaF...
March 30, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28325510/increased-nuclear-localization-of-substance-p-in-human-gastric-tumor-cells
#19
Miguel Muñoz, Marisa Rosso, Andrés Carranza, Rafael Coveñas
Gastric cancer (GC) is an aggressive disease that remains the fourth most common type of cancer and is the second leading cause of cancer-related death worldwide. Treatment of advanced or metastatic GC has seen little progress and median overall survival in this group remains <1 year. It is urgent to investigate new mechanisms to understand GC progression. It is known that substance P (SP), after binding to the neurokinin-1 (NK-1) receptor, elicits GC proliferation; that GC cells and samples express NK-1 receptors; that NK-1 receptor antagonists, in a concentration dependent manner, inhibit the proliferation of GC cells and that these cells die by apoptosis...
March 18, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28314612/a-simple-imagej-macro-tool-for-analyzing-mitochondrial-network-morphology-in-mammalian-cell-culture
#20
Andrew J Valente, Lucas A Maddalena, Ellen L Robb, Fereshteh Moradi, Jeffrey A Stuart
Mitochondria exist in a dynamic cycle of fusion and fission whose balance directly influences the morphology of the 'mitochondrial network', a term that encompasses the branched, reticular structure of fused mitochondria as well as the separate, punctate individual organelles within a eukaryotic cell. Over the past decade, the significance of the mitochondrial network has been increasingly appreciated, motivating the development of various approaches to analyze it. Here, we describe the Mitochondrial Network Analysis (MiNA) toolset, a relatively simple pair of macros making use of existing ImageJ plug-ins, allowing for semi-automated analysis of mitochondrial networks in cultured mammalian cells...
March 14, 2017: Acta Histochemica
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