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Acta Histochemica

Victor A Shahen, Laurence C Cantrill, Nasim Bahram Sangani, John Christodoulou, Wendy A Gold
Mitochondria are crucial for cells, supplying up to 90% of the energy requirements for neurons. Their correct localisation is crucial and ensured by a transport system. Mitochondrial trafficking in neurons is particularly critical, because mitochondria must leave the soma and travel along the axon and dendritic network to facilitate neuronal function. Abnormal mitochondrial trafficking has been reported in several neurological disorders, therefore the ability to quantify and analyse mitochondrial trafficking is vital to improving our understanding of their pathogenesis...
September 14, 2018: Acta Histochemica
Carina Guidi Pinto, Kátia Colombo Marchi, Ailton Amarante Arizza, Ana Paula Silveira Leite, Carlos Renato Tirapelli, Selma Maria Michelin Matheus
The chronic use of ethanol causes neuropathy and atrophy of type II fibers and promotes vitamin D decrease. This study evaluated cholecalciferol effects on the deep fibular nerve and extensor digitorum longus (EDL) muscle using an UChB ethanol-preferring rats model. Blood analyses were carried out to measure levels of 25-hydroxycholecalciferol (25(OH)D), calcium (Ca2+ ), Phosphorus (P), and parathyroid hormone (PTH). It was used EDL muscle to evaluate oxidative stress. The deep fibular nerve and EDL muscle were used for morphologic and morphometric assessment...
September 14, 2018: Acta Histochemica
Katarina Vitošević, Miloš Todorović, Tatjana Varljen, Živana Slović, Stevan Matić, Danijela Todorović
The aim of this study is to investigate the effects of formalin fixation on the degradation of DNA molecules in five different healthy tissues exempted during the autopsy, as well as the selection of the method that is most suitable for the DNA isolation. Heart muscle, liver, brain, lung and kidney tissue obtained from the healthy people who suddenly died from a violent death were used. The parts of tissue were fixed in 10% phosphate-buffered formalin as well as in 4% unbuffered formalin at room temperature...
September 14, 2018: Acta Histochemica
Luiz Henrique Florindo, Vinicius Araújo Armelin, David John McKenzie, Francisco Tadeu Rantin
This review considers the environmental and systemic factors that can stimulate air-breathing responses in fishes with bimodal respiration, and how these may be controlled by peripheral and central chemoreceptors. The systemic factors that stimulate air-breathing in fishes are usually related to conditions that increase the O2 demand of these animals (e.g. physical exercise, digestion and increased temperature), while the environmental factors are usually related to conditions that impair their capacity to meet this demand (e...
September 12, 2018: Acta Histochemica
Mehmet Özbek, Mehmet Fatih Bozkurt, Feyzullah Beyaz, Emel Ergün, Levent Ergün
The enteric nervous system (ENS) is a network of neurons and glia found in the gut wall and governs this gastrointestinal function independently from the central nervous system (CNS). ENS comprises the myenteric plexus (MP) and the submucous plexus (SP). In this study, we examined the expression profile of neurofilament heavy chain (NF-H), neuron-specific enolase (NSE), calcyclin (S100A6), vimentin and glial fibril acidic protein (GFAP) in ovine ileal enteric neurons and enteric glia cells (EGCs) during prenatal development using an immunohistochemical method...
September 11, 2018: Acta Histochemica
John N Maina
The evolution of air-breathing and transition from water to land were pivotal events that greatly determined the ecological diversification, the advances and the successes of animal life. During their relocation onto land, the so-called bimodal breathers were literally caught at the water-air interface. Among such animals are the diverse air-breathing bony fish. Such taxa, however, strictly do not constitute the so-called 'bridging animals', i.e., the inaugural animals that crossed from water to land, nor are they their direct progenitors...
September 5, 2018: Acta Histochemica
Bruno Tota, Daniela Amelio, Maria Carmela Cerra, Filippo Garofalo
This review aims to summarize the changes of the NOS/NO system which occur in the lungs, gills, kidney, heart, and myotomal muscle of air breathing fish of the genus Protopterus, i.e. P. dolloi and P. annectens, in relation to the switch from freshwater to aestivation, and vice-versa. The modifications of NOS and its partners Akt and Hsp-90, and HIF-1α, detected by immunohistochemical and molecular biology methods, are discussed together with the apoptosis rate, evaluated by TUNEL. We hypothesize that these molecular components are key elements of the stress-induced signal transduction/integration networks which allow the lungfish to overcome the dramatic environmental challenges experienced at the beginning, during, and at the end of the dry season...
September 5, 2018: Acta Histochemica
Elaheh Nahari, Mazdak Razi
The present prospective study was done to evaluate the effect of silymarin (SMN) on endometriotic-like legions establishment and growth in experimentally-induced endometriosis. For this purpose, the experimental endometriosis was induced in 12 rats and then the animals subdivided into endometriosis-sole and SMN (50 mg kg-1 , orally)+endometriosis groups. Following 28 days, the legions establishment, size, Glial cell line-derived neurotrophic factor (GDNF), gfrα1, B Cell Lymphoma 6 (Bcl-6b), Bcl-2, extracellular regulator kinase (ERK1/2) expression ratios, angiogenesis, the apoptosis and fibrosis indices were investigated...
September 5, 2018: Acta Histochemica
Katarina Vukojevic, Fila Raguz, Marijan Saraga, Natalija Filipovic, Ivana Bocina, Darko Kero, Merica Glavina Durdov, Vlatka Martinovic, Mirna Saraga-Babic
BACKGROUND: Podocytes are postmitotic, highly specialized cells which maintain the glomerular filtration barrier (GFB). Their injury is characterized by foot processes effacement and change in protein expression leading to proteinuria and end-stage kidney disease. METHODS: Our study focuses on the morphological and immunohistochemical changes of human podocytes during normal development and postnatal period, compared to congenital nephrotic syndrome of the Finnish type (CNF)...
September 4, 2018: Acta Histochemica
Yuen K Ip, Shit F Chew
During water-land transition, ancient fishes acquired the ability to breathe air, but air-breathing engendered problems in nitrogenous waste excretion. Nitrogen is a fundamental component of amino acids, proteins, and nucleic acids, and the degradation of these nitrogen-containing compounds releases ammonia. Ammonia is toxic and must be removed. Fishes in water excrete ammonia as the major nitrogenous waste through gills, but gills of air-breathing fishes are modified for air-breathing or largely replaced by air-breathing organs...
September 1, 2018: Acta Histochemica
José M Icardo
This paper summarizes the main morphological tracts exhibited by lungs and gas bladders in fishes. The origin and organ location, the presence of a glottal region, the inner architecture, the characteristics of the exchange barrier and the presence of pulmonary arteries have been reviewed in the two types of air-breathing organs. With the exception of the dorsal (bladders) or ventral (lungs) origin from the posterior pharynx, none of the morphological traits analyzed can be considered specific for either lungs or gas bladders...
August 31, 2018: Acta Histochemica
Bernd Pelster, Chris M Wood
Aquatic areas frequently face hypoxic conditions. In order to get sufficient oxygen to support aerobic metabolism, a number of freshwater fish resort to aerial respiration to supplement gill respiration especially in situations with reduced oxygen availability in the water. In many species a concomitant reduction in gill surface area or in gill perfusion reduces possible loss of aerially acquired oxygen to the water at the gills, but it also compromises the ion regulatory capacity of gill tissue. In consequence, the reduced gill contact area with water requires appropriate compensation to maintain ion and acid-base homeostasis, often with important ramifications for other organs...
August 31, 2018: Acta Histochemica
Michael G Jonz
Respiratory chemoreceptors in vertebrates are specialized cells that detect chemical changes in the environment or arterial blood supply and initiate autonomic responses, such as hyperventilation or changes in heart rate, to improve O2 uptake and delivery to tissues. These chemoreceptors are sensitive to changes in O2 , CO2 and/or H+ . In fish and mammals, respiratory chemoreceptors may be additionally sensitive to ammonia, hypoglycemia, and numerous other stimuli. Thus, chemoreceptors that affect respiration respond to different types of stimuli (or modalities) and are considered to be "polymodal"...
August 30, 2018: Acta Histochemica
William K Milsom
The diversity of sites and surfaces that are utilized for gas transfer from air to blood in fish is remarkable. While a few species do utilize their gills for gas exchange in air, this is a rare occurrence and most air-breathing fish utilize other surfaces including air-breathing organs and lungs. At present almost nothing is known about the central sites that initiate and regulate air breathing although hypotheses can be put forward based on our rudimentary understanding of the sites involved in water breathing in lampreys and teleost fishes, and those involved in air breathing in pre-metamorphic anuran ampibians...
August 30, 2018: Acta Histochemica
Olga Manzhulo, Anna Tyrtyshnaia, Yulia Kipryushina, Inessa Dyuizen, Igor Manzhulo
Docosahexaenoic acid (DHA, 22:6 (n-3)) leads to recovery of locomotor functions observed of spinal cord injury (SCI) in rats. In present study, we characterized the expression of iba-1, CD86, CD163 in microglia/macrophages, to assess activation state and M1 (pro-inflammatory)/M2 (anti-inflammatory) phenotypes respectively, in the rostral, central and caudal segment of the spinal cord on 7 and 35 days after SCI. We found that DHA treatment leads to: (1) an increased activation and proliferation of microglial cells; (2) an alteration in the dynamics between M1 and M2 microglia/macrophages phenotypes (3) and increased production of an antioxidant enzymes...
August 28, 2018: Acta Histochemica
Ling Zhou, Xuping Niu, Jiannan Liang, Junqin Li, Jiao Li, Yueai Cheng, Yanfeng Meng, Qiang Wang, Xiaoli Yang, Gang Wang, Yu Shi, Erle Dang, Kaiming Zhang
OBJECTIVE: To directionally-differentiate dermis-derived mesenchymal stem cells (DMSCs) into vascular endothelial cells (VECs) in vitro, providing an experimental basis for studies on the pathogenesis and treatment of vascular diseases. METHODS: After separation by adherent culture, VEC line supernatant, vascular endothelial growth factor (VEGF), bone morphogenetic protein-4 and hypoxia were used for the differentiation of VECs from DMSCs. The cell type was authenticated by flow cytometry, matrigel angiogenesis assay in vitro, and immunofluorescent staining during differentiation...
August 21, 2018: Acta Histochemica
Tuğba Özaydın, Yasemin Öznurlu, Emrah Sur, İlhami Çelik, Deniz Uluışık
The effects of bisphenol A on the some plasma cytokine levels and distribution of CD8+ and CD4+ T lymphocytes in spleen, ilealPeyer's patch and bronchus-associated lymphoid tissue in rats were investigated. A total of fourty male Wistar Albino rats were divided into five groups including 8 rats in each one: control, vehicle, BPA 5, BPA 50 and BPA 500 groups. Doses of 5, 50 and 500 μg/kg BPA were dissolved in ethanol, then mixed with corn oil. The control group received no treatment. The vehicle group was given the ethanol-corn oil mixture...
August 11, 2018: Acta Histochemica
Marvin Siegfried Oswald, Martin-Leo Hansmann
Lymph node diagnostics are essentially based on cutting thin sections of formalin fixed tissues. After hematoxylin and eosin stain, Giemsa stain and immunohistochemical staining of these tissues, the lymph node diagnosis is done using a light microscope, looking at two-dimensional pictures. Three-dimensional visualizations of lymph node tissue have not been used in lymphoma diagnostics yet. This article describes three-dimensional visualization of lymphoid tissue, using thick paraffin sections, immunostained with monoclonal antibodies, confocal laser scanning and data processing with appropriate software and the 3D printing process itself...
August 10, 2018: Acta Histochemica
Lina Qi, Nannan Guo, Quanwei Wei, Pengjin Jin, Wei Wang, Dagan Mao
The nuclear receptor 4A (NR4A) members play important roles in cellular proliferation, differentiation and apoptosis. The current study first evaluate the expression of ovarian NR4A1 during different luteal stages in rats. Immature rats aged 28 days were treated with sequential Pregnant mare serum gonadotropin (PMSG) (D -2) / human chorionic gonadotropin (hCG) (D 0) to induce pseudopregnancy. Serum progesterone (P4 ) and ovarian expression of NR4A1 were detected by RIA and WB, respectively, at follicle stage (D 0), early (D 2), middle (D 7) and late (D 14 and D 20) luteal stages...
August 7, 2018: Acta Histochemica
Tanongsak Laowanitwattana, Sirinda Aungsuchawan, Suteera Narakornsak, Runchana Markmee, Waleephan Tancharoen, Junjira Keawdee, Nonglak Boonma, Witoon Tasuya, Lamaiporn Peerapapong, Nathaporn Pangjaidee, Peeraphan Pothacharoen
Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest...
August 2, 2018: Acta Histochemica
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