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Acta Histochemica

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https://www.readbyqxmd.com/read/29778238/interplay-between-estrogen-related-receptors-and-steroidogenesis-controlling-molecules-in-adrenals-in-vivo-and-in-vitro-study
#1
A Pacwa, E Gorowska-Wojtowicz, A Ptak, P Pawlicki, A Milon, M Sekula, K Lesniak, B Bilinska, A Hejmej, M Kotula-Balak
Estrogen-related receptors (ERRs) α, β and γ appear to be novel molecules implicated in estrogen signaling. We blocked and activated ERRs in mouse (C57BL/6) adrenals and adrenocortical cells (H295R) using pharmacological agents XCT 790 (ERRα antagonist) and DY131 (ERRβ/γ agonist), respectively. Mice were injected with XCT 790 or DY131 (5 μg/kg bw) while cells were exposed to XCT 790 or DY131 (0.5 μg/L). Irrespectively of the agent used, changes in adrenocortical cell morphology along with changes in lutropin, cholesterol levels and estrogen production were found...
May 16, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29776745/anatomical-histological-and-immunohistochemical-study-of-testicular-development-in-columba-livia-aves-columbiformes
#2
G B Olea, M V Aguirre, D M Lombardo
In this work, testicular ontogeny is analyzed at the anatomical, histological and immunohistochemical levels; the latter through the detection of GnRHR and PCNA in the testicles of embryos, neonates and juveniles of Columba livia. We analyzed 150 embryos, 25 neonates and 5 juveniles by means of observations under a stereoscopic magnifying glass and scanning electron microscope (SEM). The histological analysis was performed using hematoxylin-eosin staining techniques and the PAS reaction. For the immunohistochemical analysis, the expression of GnRHR and PCNA in embryos corresponding to stages 41, 43 and in neonates of 2, 5, 7 and 75 days post-hatch was revealed in testicular histological preparations...
May 16, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29773228/changes-in-mucins-and-matrix-metalloproteases-in-the-endometrium-of-early-pregnant-alpacas-vicugna-pacos
#3
Daniela E Barraza, Renato Zampini, Silvana A Apichela, Joel I Pacheco, Martin E Argañaraz
South American Camelids (SAC) have unique reproductive features, one of which is that 98% of the pregnancies develop in the left uterine horn. Furthermore, early pregnancy is an uncharacterized process in these species, especially in regard to the ultrastructural, biochemical and genetic changes that the uterine epithelial surface undergoes to allow embryo implantation. The present study describes the uterine horn luminal surface and the characteristics of the mucinous glycocalyx in non-pregnant and early pregnant (15 days) female alpacas...
May 14, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29759662/nandrolone-decanoate-and-physical-activity-affect-quadriceps-in-peripubertal-rats
#4
Jasmina Sretenovic, Vladimir Ajdzanovic, Vladimir Zivkovic, Ivan Srejovic, Milena Corbic, Verica Milosevic, Vladimir Jakovljevic, Zoran Milosavljevic
Anabolic androgenic steroids (AASs) are synthetic analogs of testosterone often used by athletes to increase the skeletal muscle mass. Our goal was to examine the effects of physical activity and physical activity combined with supraphysiological doses of nandrolone on functional morphology of the quadriceps muscle. The study included 32 peripubertal Wistar rats, divided into 4 groups: control (T-N-), nandrolone (T-N+), physical activity (T+N-) and physical activity plus nandrolone (T+N+) groups. The T+N- and T+N+ group swam for 4 weeks, 1 h/day, 5 days/week...
May 11, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29754696/follicle-stimulating-hormone-receptor-protein-is-expressed-in-ovine-uterus-during-the-estrous-cycle-and-utero-placenta-during-early-pregnancy-an-immunohistochemical-study
#5
Anna T Grazul-Bilska, Arshi Reyaz, Veselina Valkov, Sheri T Dorsam, Dale A Redmer
Follicle stimulating hormone (FSH) is a well characterized gonadotropin that controls primarily development and functions of ovarian follicles in mammalian species. FSH binds to a specific G protein-coupled receptor (FSHR) belonging to the glycoprotein hormone receptor family that plays an essential role in reproduction. Although the primary location of FSHR is in the gonads (mainly in ovarian follicles), FSHR protein and/or mRNA have also been detected in extragonadal female reproductive tissues including embryo, placenta, endometrium, cervix, ovarian cancer tissues, and/or endometriotic lesions in several species...
May 11, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29751963/ameliorative-effects-of-bone-marrow-derived-pancreatic-progenitor-cells-on-hyperglycemia-and-oxidative-stress-in-diabetic-rats
#6
Hamdy Rizk, A F Tohamy, Walaa Mohamed Sayed, Abdelbary Prince
The present study aimed to investigate the effects of Bone marrow derived pancreatic progenitor cells (BM- PPCs) in diabetic rats. It was conducted on 30 adult male Sprague-Dawley rats weighing 200-220 g. They were divided into three groups: (a) Group 1 was the control group; (b) Group 2 was the diabetic (induced diabetic by a single intraperitoneal (IP) injection of streptozotocin (STZ) (60 mg/kg) and (c) Group 3 was the treated (received injection of 2.5 X 106 BM- PPCs via the tail vein twice with a 21-day time interval)...
May 8, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29739626/multiple-immunolabeling-with-antibodies-from-the-same-host-species-in-combination-with-tyramide-signal-amplification
#7
Igor Buchwalow, Vera Samoilova, Werner Boecker, Markus Tiemann
A general problem in immunocytochemistry is the development of a reliable multiple immunolabeling method with primary antibodies originating from the same host species. When primary antibodies are raised in the same host species, the secondary species-specific antibodies can cross-react with each of the primary antibodies. This obstacle can however be avoided with the use of striping buffers eluting the primary/secondary antibody complex. After elution of the previous primary/secondary antibody complex, the next primary antibody from the same host species can be applied...
May 5, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29724455/telocytes-in-human-fetal-skeletal-muscle-interstitium-during-early-myogenesis
#8
Mirca Marini, Mirko Manetti, Irene Rosa, Lidia Ibba-Manneschi, Eleonora Sgambati
A new peculiar stromal cell type called telocyte (TC)/CD34-positive stromal cell (i.e. cell with distinctive prolongations named telopodes) has recently been described in various tissues and organs, including the adult skeletal muscle interstitium of mammals. By forming a resident stromal three-dimensional network, TCs have been suggested to participate in different physiological processes within the skeletal muscle tissue, including homeostasis maintenance, intercellular signaling, tissue regeneration/repair and angiogenesis...
April 30, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29685720/immunohistochemical-expression-of-apoptosis-related-biomarkers-in-normal-tissues-of-camel-camelus-dromedarius-a-survey-in-a-desert-dwelling-mammalian-model
#9
Abdel-Hamid K Osman, Thomas Caceci, Mitchiko Shintani
Programmed cell death is a fundamental event that takes place during organ development and plays an important role in cellular homeostasis. Since various body organs of the camel are under high ecological and physiological stress during food and water deprivation, desiccation, and the long exposure to solar radiation in these desert nomads, we aimed to examine the immunohistochemical expression of apoptosis-related biomarkers in some of its normal body organs to illustrate a basic track for further pathological investigation...
April 20, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29655854/the-expression-and-distribution-of-a-leptin-receptor-in-the-central-nervous-system-digestive-organs-and-gonads-of-the-giant-freshwater-prawn-macrobrachium-rosenbergii
#10
Jaruwan Poljaroen, Ruchanok Tinikul, Panat Anuracpreeda, Prasert Sobhon, Yotsawan Tinikul
In the present study, the presence and distribution of leptin receptor (LEP-R) in central nervous system, digestive organs, gonads of giant freshwater prawn, Macrobrachium rosenbergii, were investigated with Western blot and immunohistochemistry. By Western blot a LEP-R with a molecular weight (MW) of 100 kDa was detected in the brain, thoracic ganglia, abdominal ganglia, hepatopancreas, all parts of the gastrointestinal tract, ovaries, and testes. In hepatopancreas and foregut, another intense positive band was detected at molecular weight of 30 kDa, which could be an isotype of LEP-R...
April 11, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29628120/immunomorphometric-variations-of-sustentacular-cells-of-the-male-viscacha-adrenal-medulla-during-the-annual-reproductive-cycle-effects-of-androgens-and-melatonin
#11
Luis Ezequiel Gallol, Fabian Heber Mohamed
The adrenal medulla is crucial for the survival of species facing significant environmental changes. The parenchyma is composed mainly of chromaffin cells, ganglion cells and sustentacular cells (SC). The male viscacha exhibits seasonal variations of gonadal activity and other metabolic functions. The aim of this work was to investigate the influence of the reproductive conditions on the morphology of SC of this rodent. In addition, the effects of testosterone and melatonin on these cells were studied. Immunoexpression of S100 protein, GFAP and vimentin were analyzed...
April 5, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29622345/early-islets-and-mesenchyme-from-an-injured-adult-pancreas-improve-syngeneic-engraftments-and-islet-graft-function-in-diabetic-rats
#12
Venant Tchokonte-Nana, Juziel Kampando Manda
A decrease in mass of isografts and a decline in islet function are major challenges in islet transplantations. Despite this, transplantation of 84 h harvested pancreatic duct ligation (PDL) tissues have been shown to have the same functional ability to foetal pancreata, but there was only 40% success in reverting hyperglycaemia. We tested the potential of early islets with mesenchymal stromal cells (MSCs) to promote isogeneic grafts survival and to restore normoglycemia in diabetic rats, in comparison with late islets...
April 2, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29606555/current-methodology-of-mtt-assay-in-bacteria-a-review
#13
REVIEW
Ewa Grela, Joanna Kozłowska, Agnieszka Grabowiecka
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay is a popular tool in estimating the metabolic activity of living cells. The test is based on enzymatic reduction of the lightly colored tetrazolium salt to its formazan of intense purple-blue color, which can be quantified spectrophotometrically. Under properly optimized conditions the obtained absorbance value is directly proportional to the number of living cells. Originally, the MTT assay was devised for use in eukaryotic cells lines and later applied for bacteria and fungi...
March 29, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29605225/histomorphological-changes-in-the-pancreas-and-kidney-and-histopathological-changes-in-the-liver-in-male-wistar-rats-on-antiretroviral-therapy-and-melatonin-treatment
#14
Danélle Truter, Nireshni Chellan, Hans Strijdom, Ingrid Webster, Jordyn Rawstorne, Sanet H Kotzé
Combination antiretroviral therapy (cART) has shown to cause inflammation, cellular injury and oxidative stress, whereas melatonin has been successful in reducing these effects. The aim of the study was to determine potential morphometric changes caused by cART in combination with melatonin supplementation in human immunodeficiency virus (HIV)-free rats. Tissue samples (N = 40) of the pancreas, liver and kidney from a control (C/ART-/M-), cART group (C/ART + ), melatonin (C/M + ) and experimental group (ART+/M + ) were collected and stained with haematoxylin and eosin (H&E) and evaluated for histopathology...
March 28, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29559175/gypenosides-attenuate-lipopolysaccharide-induced-optic-neuritis-in-rats
#15
Fang Wang, Yalong Dang, Jing Wang, Ting Zhou, Yu Zhu
PURPOSE: To evaluate the effect of gypenosides (GPs) on lipopolysaccharide (LPS)-induced optic neuritis rats. METHODS: Optic neuritis was induced by a single microinjection of LPS into the optic nerve of Sprague Dawley rats. GPs (400 mg/kg) was administrated by gavage for 21 days. The optic nerve structure changes and demyelination were observed after hematoxylin & eosin and Luxol-fast blue staining. Apoptosis of retinal ganglion cells (RGCs) was evaluated using Brn3a-TUNEL double staining...
March 17, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29764621/erratum-to-the-possible-protective-role-of-pumpkin-seed-oil-in-an-animal-model-of-acid-aspiration-pneumonia-light-and-electron-microscopic-study-acta-histochemica-119-2017-161-171
#16
https://www.readbyqxmd.com/read/29551457/highly-specific-detection-of-muscarinic-m3-receptor-g-protein-interaction-and-intracellular-trafficking-in-human-detrusor-using-proximity-ligation-assay-pla
#17
Mandy Berndt-Paetz, Luise Herbst, Annett Weimann, Andreas Gonsior, Jens-Uwe Stolzenburg, Jochen Neuhaus
PURPOSE: Muscarinic acetylcholine receptors (mAChRs) regulate a number of important physiological functions. Alteration of mAChR expression or function has been associated in the etiology of several pathologies including functional bladder disorders (e.g bladder pain syndrome/interstitial cystitis - BPS/IC). In a previous study we found specific mAChR expression patterns associated with BPS/IC, while correlation between protein and gene expression was lacking. Posttranslational regulatory mechanisms, e...
May 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29534834/immunohistochemical-localization-of-osteoblast-activating-peptide-in-the-mouse-kidney
#18
Ahmed E Noreldin, Yaser Hosny Ali Elewa, Yasuhiro Kon, Katsuhiko Warita, Yoshinao Z Hosaka
Osteoblast activating peptide (OBAP) is a newly discovered peptide detected in the rat stomach, which has a major role in osteogenesis. Recently, we revealed its localization in the parietal cells of the rat stomach. There have been no data regarding OBAP expression in the kidney, despite its role in calcium reabsorption in renal tubules. The current study aimed to inspect the expression of OBAP in the kidney of twelve 10-week-old male C3H/HeNJc1 mice using immunohistochemistry, and immunoelectron microscopic localization...
May 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29526379/an-immunohistochemical-and-ultrastructural-analysis-of-the-retina-in-tadalafil-cialis-treated-rats
#19
Nahla Reda Sarhan, Nesreen Moustafa Omar
Tadalafil (Cialis) is one of the most commonly used phosphodiesterase type5 (PDE5) inhibitors. This work aimed to analyze the histological and ultrastructural changes provoked by chronic tadalafil administration in the rat retina, correlate between such changes and PDE5 immunoexpression and to evaluate the possible reversibility of these changes. Thirty Sprague Dawley male rats were randomly distributed into 3 groups. Control group; given 1 ml distilled water daily for 6 weeks. Tadalafil group; given tadalafil in a daily dose of 2...
May 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29598902/retraction-notice-to-cd133-cd44-oct4-nestin-stem-like-cells-isolated-from-panc-1-cell-line-may-contribute-to-multi-resistance-and-metastasis-of-pancreatic-cancer-acta-histochemica-115-2013-349-356
#20
Dongqing Wang, Haitao Zhu, Ying Zhu, Yanfang Liu, Huiling Shen, Ruigen Yin, Zhijian Zhang, Zhaoliang Su
No abstract text is available yet for this article.
April 2018: Acta Histochemica
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