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Acta Histochemica

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https://www.readbyqxmd.com/read/28923316/different-combinations-of-maternal-and-postnatal-diet-are-reflected-in-changes-of-hepatic-parenchyma-and-hepatic-tnf-alpha-expression-in-male-rat-offspring
#1
Željka Perić Kačarević, Anđela Grgić, Darija Šnajder, Nikola Bijelić, Tatjana Belovari, Olga Cvijanović, Valerija Blažičević, Radivoje Radić
Obesity is related to increased TNF-alpha production in different tissues. TNF-alpha is connected to mitochondrial dysfunction in the liver and also development of fatty infiltration of the liver. Also, postnatal change from normal to high-fat diet causes a significant increase in TNF-alpha serum levels. The aim of this research was to determine how maternal diet and switching male offspring to a different dietary regime after lactation influences rat liver. Ten female Sprague Dawley rats at nine weeks of age were randomly divided in two groups and fed either standard laboratory chow or high-fat diet during six weeks, and then mated with the same male subject...
September 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28919179/effects-of-nest-deprivation-on-hypothalamic-mesotocin-in-incubating-native-thai-hens-gallus-domesticus
#2
Panpradap Sinpru, Tom E Porter, Mohamed E El Halawani, Yupaporn Chaiseha
Avian mesotocin (MT) is homologous to oxytocin in mammals. Native Thai chickens (Gallus domesticus) strongly express maternal behaviors including incubation and rearing. However, the role of MT during incubation behavior has never been studied. The objective of this study was to determine the physiological function(s) of the MTergic system in incubation behavior in native Thai chickens. The brains were collected from incubating (INC) and nest-deprived (ND) hens at different time points (days 3, 6, 8, 10, 14, 18, and 21; n=6)...
September 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28919178/the-presence-of-abalone-egg-laying-hormone-like-peptide-in-the-central-nervous-system-and-ovary-of-the-spotted-babylon-babylonia-areolata
#3
Jirawat Saetan, Piyakorn Boonyoung, Uraporn Vongvatcharanon, Thanapong Kruangkum, Kanjana Khornchatri, Pinij Thaweethamsewee, Prasert Sobhon, Prapee Sretarugsa
Recently, the neuronal classification of the ivory shell Spotted Babylon, Babylonia areolata, was readily demonstrated. Regarding its importance as marine economic molluscan species, the attempt to understand the neuroendocrine regulation is necessary. This study firstly demonstrated the neurosecretory cells as well as the existence and distribution of the egg-laying hormone (ELH)-like peptide in the central nervous system (CNS) and ovary of the B. areolata. The neurosecretory cell was characterized by the cytoplasmic purple dot-like structure as stained by the Gomori's paraldehyde fuchsin...
September 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28912046/seasonal-expression-of-luteinizing-hormone-receptor-and-follicle-stimulating-hormone-receptor-in-testes-of-the-wild-ground-squirrels-citellus-dauricus-brandt
#4
Junjie Wang, Ying Wang, Manyu Zhu, Fengwei Zhang, Xia Sheng, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng
The objective of this study was to evaluate whether luteinizing hormone (LH), follicle stimulating hormone (FSH) and their receptors luteinizing hormone receptor (LHR) and follicle stimulating hormone receptor (FSHR) play roles in the seasonal spermatogenesis of the wild ground squirrels. To that end, we characterized the testicular immunolocalization of LHR and FSHR, their expression on both mRNA and protein levels, as well as serum concentrations of LH and FSH in male wild ground squirrels throughout the annual reproductive cycle...
September 11, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28886869/immunohistochemical-study-of-arginases-1-and-2-in-the-olfactory-bulbs-of-the-korean-roe-deer-capreolus-pygargus
#5
Jeongtae Kim, Meejung Ahn, Yuna Choi, Ji-Yeon Hyeon, Taekyun Shin
Arginases are enzymes of the urea cycle that catalyze the hydrolysis of l-arginine to ornithine and urea. The enzymes are core components of the arginine-ornithine-glutamate-γ-amino butyric acid pathway of the central nervous system. In the present study, we immunohistochemically determined the localization of arginase 1 and 2 in the olfactory bulb (OB) of the roe dear (Capreolus pygargus). Reverse transcription PCR revealed that the mRNAs encoding both arginase 1 and 2 were expressed in the OB. Arginase 1 was localized to olfactory nerve axons, calcitonin gene-related peptide-positive mitral/tufted cells (excitatory neurons), and glutamate acid decarboxylase 65/67-immunopositive periglomerular cells of the main olfactory bulb...
September 5, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28867597/overexpression-of-suppressors-of-cytokine-signaling-1-regulate-the-proliferation-and-differentiation-of-rat-derived-neural-stem-cells
#6
Meng Cui, Xin-Long Ma, Jie Sun, Jin-Quan He, Lin Shen, Fang-Guo Li
Neural stem cells are a reliable resource in various neural tissue repair and neurodegenerative diseases. Increasing evidence has demonstrated that Suppressor of cytokine signaling proteins (SOCS) was involved in the nervous system development. The universality and diversity of SOCS also suggested their important roles in neurogenesis and nerve regeneration. In this study, we employed a lentiviral vector to investigate the impacts of overexpression SOCS1 on the proliferation and differentiation of rat-derived NSCs...
August 31, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28851482/bioinformatics-analyses-of-pathways-and-gene-predictions-in-il-1%C3%AE-and-il-1%C3%AE-knockout-mice-with-spinal-cord-injury
#7
Zhuangchen Zhu, Defeng Wang, Wei Jiao, Guang Chen, Yan Cao, Qingfu Zhang, Junqin Wang
PURPOSE: This study aimed to explore the potential genes and pathways regulated in spinal cord injury (SCI) model mice with IL-1α and IL-1β knockout (KO). METHODS: Gene expression profile GSE70302, which includes data from injured spinal cord of 4 IL-1α-KO mice, 4 IL-1β-KO mice and 4 C57BL with 6 mice as controls was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) of the IL-1α-KO or IL-1β-KO vs. control, and IL-1α-KO vs...
August 26, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28847601/mesenchymal-cells-are-required-for-epithelial-duct-cell-to-beta-cell-maturation-and-function-in-an-injured-adult-pancreas-in-the-rat
#8
Juziel Kampando Manda, Benedict John Page, Venant Tchokonte-Nana
The islet, the endocrine portion of the pancreas - develops from an invagination of the pancreatic duct epithelial cells (PDECs) into the surrounding tissue. The contact of the PDECs with mesenchymal cells (MSCs) may be an essential drive for endocrine cell fate. During pancreatic development, cells that express Neurogenin-3 (Ngn3) biomarker are precursors of insulin- producing beta cells. These precursors have been reported in the neogenesis of islets from adult tissues following the surgical ligation of the main pancreatic duct (PDL)...
August 25, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28823522/gonadal-morphogenesis-and-establishment-of-the-germline-in-the-phyllostomid-bat-sturnira-lilium
#9
Tania Janeth Porras-Gómez, Adriana Martínez-Juárez, Norma Moreno-Mendoza
In vertebrates such as the mouse and the human, primordial germ cells (PGCs) arise at the base of the allantois and are carried to the epithelium of the posterior intestine, to later migrate to the primordial gonads. In the case of bats, almost nothing is known about this process. To clarify the dynamics of PGCs during gonadal morphogenesis in the phyllostomid bat Sturnira lilium, the proteins for the Ddx4, Sox9 and Mis genes were detected in the gonads of embryos at different stages of development. We identified 15 stages (St) of embryonic development in Sturnira lilium...
August 17, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28803749/the-effects-of-17%C3%AE-estradiol-on-blood-brain-barrier-integrity-in-the-absence-of-the-estrogen-receptor-alpha-an-in-vitro-model
#10
Serap Erdem Kuruca, Sabriye Karadenizli, Kadriye Akgun-Dar, Aysegul Kapucu, Zulal Kaptan, Gulay Uzum
The blood-brain barrier (BBB), which saves the brain from toxic substances, is formed by endothelial cells. It is mainly composed of tight junction (TJ) proteins existing between endothelial cells. Estrogen is an important regulatory hormone of BBB permeability. It protects the BBB before menopause, but may increase BBB permeability with aging. In addition, nitric oxide modulates BBB permeability. Alcohol impairs the integrity of the BBB with oxidants and inflammatory mediators such as iNOS. We investigated the effects of estrogen on BBB integrity in an in vitro BBB model created with ERα-free HUVEC (human umbilical vein endothelial-like cells) to mimics the menopausal period...
August 10, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28784287/the-impact-of-aged-garlic-extract-on-adriamycin-induced-testicular-changes-in-adult-male-wistar-rats
#11
Ashraf Youssef Nasr
Adriamycin (ADR), a potent cytotoxic drug, has many adverse effects on different body organs limiting its therapeutic uses. Aged garlic extract (AGE), a garlic preparation, contains many organosulfur compounds with potent antioxidant activity. This investigation was conducted to study the possible protective effects & the underlying the mechanism of AGE on ADR-induced testicular damage. Thirty-six adult male rats were assigned into six groups: a control, AGE-treated (250mg/kg once oral for 14days), ADR-treated (10mg/kg, i...
August 5, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28780960/expression-of-glucose-transporter-8-glut8-in-spermatogenesis-of-adult-boar-testes
#12
Huaming Xi, Zhen Zhang, Rui Ji, Hui Shen, Xiaorui Fan, Qinghong Li, Xiyun Bai, Zhixue Cheng, Junping He
The present study was designed to investigate the glucose transporter 8 (GLUT8) expression and localization in adult boar testis. Localization and expression of GLUT8 were conducted with Western Blotting, immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) methods GLUT8 protein and mRNA were expressed in the boar testes. The results of Western Blotting analysis showed specificity of the antibody for protein of boar testes. The immunohistochemistry results showed that GLUT8 protein mainly localized in spermatocytes, round spermatids and elongated spermatids of the seminiferous tubules in the adult boar testes...
August 3, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28732677/assessment-of-bone-repair-in-critical-size-defect-in-the-calvarium-of-rats-after-the-implantation-of-tricalcium-phosphate-beta-%C3%AE-tcp
#13
Leonardo de Freitas Silva, Erik Neiva Ribeiro de Carvalho Reis, Tânia Aparecida Barbara, João Paulo Bonardi, Idelmo Rangel Garcia, Paulo Sérgio Perri de Carvalho, Daniela Ponzoni
OBJECTIVES: Evaluating the osteoconductive property of tricalcium phosphate beta (β-TCP) in comparison to that of inorganic bovine bone for repair in a critical-size defect in the rat calvarium. MATERIALS AND METHODS: Critical-size defects of 7mm were made with a trephine in the calvaria of 48 Wistar rats. The animals were divided into four groups, and the defects in each group were filled with tricalcium phosphate beta (β-TCP), inorganic bovine bone (Bio-Oss), autogenous bone, or left empty...
July 18, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28728806/foxp3-and-tlr2-in-co-expression-in-oral-cancer
#14
LETTER
H M Hussaini, V P B Parachuru, G J Seymour, A M Rich
No abstract text is available yet for this article.
July 17, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28716188/is-toll-like-receptor-2-really-inhibiting-foxp3-tlr2-cells-and-enhancing-the-anti-tumour-response-in-oral-squamous-cell-carcinoma
#15
LETTER
Xi Zeng, Mao Li, Guang-Dong Liao, Ming-Rong Xi
No abstract text is available yet for this article.
July 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28705489/the-effects-of-estradiol-valerate-and-remifemin-on-liver-lipid-metabolism
#16
Biao Jin, Wenjuan Wang, Wenpei Bai, Jing Zhang, Ke Wang, Lihua Qin
To investigate the lipid metabolism dysregulation in the liver of ovariectomized (OVX) rats and effects of estradiol valerate (E) and remifemin (ICR) thereon, forty female Sprague-Dawley rats were randomly divided into sham-operated (SHAM), OVX, OVX+E, and OVX+ICR group. After 4 weeks' E or ICR treatment, serum estrogen, cholesterol, and triglyceride levels; lipid droplets in hepatocytes; hepatocyte morphology; and the expression of estrogen receptor α (ERα), liver X receptor (LXR), and sterol regulatory element binding proteins (SREBPs) in the liver of the rats were assessed...
July 11, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28689642/cells-in-3d-reconstitutued-eccrine-sweat-gland-cell-spheroids-differentiate-into-gross-cystic-disease-fluid-protein-15-expressing-dark-secretory-cells-and-carbonic-anhydrase-ii-expressing-clear-secretory-cells
#17
Haihong Li, Liyun Chen, Mingjun Zhang, Bingna Zhang
Secretory coils of eccrine sweat glands are composed of myoepithelial cells, dark secretory cells and clear secretory cells. The two types of cells play important roles in sweat secretion. In our previous study, we demonstrated that the 3D-reconstituted eccrine sweat gland cell spheroids differentiate into secretory coil-like structures. However, whether the secretory coil-like structures further differentiate into dark secretory cells and clear secretory cells were is still unknown. In this study, we detected the differentiation of clear and dark secretory cells in the 3D-reconstituted eccrine sweat gland cell spheroids using the dark secretory cell-specific marker, GCDFP-15, and clear secretory cell-specific marker, CAII by immunofluorescence staining...
July 6, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28739089/the-feulgen-reaction-a-brief-review-and-new-perspectives
#18
REVIEW
Maria Luiza S Mello, Benedicto de Campos Vidal
The Feulgen reaction has been proposed by Robert Feulgen and Heinrich Rossenbeck for the identification of DNA nearly a hundred years ago. Since then, many other applications of this cytochemical/topochemical procedure at qualitative and quantitative level have been proposed in relation to DNA and its role in chromatin in human, animal and plant cells. In this article, we briefly review some fundamental aspects of the Feulgen reaction and current applications of such a method in studies of altered chromatin texture, including its association with or preceding changes in transcriptional activities and effect on epigenetic marks...
July 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28624121/leptin-like-immunoreactivity-in-the-central-nervous-system-digestive-organs-and-gonads-of-the-giant-freshwater-prawn-macrobrachium-rosenbergii
#19
Jaruwan Poljaroen, Yotsawan Tinikul, Ruchanok Tinikul, Panat Anurucpreeda, Prasert Sobhon
Leptin, a highly conserved adipocyte-derived hormone, plays important roles in a variety of physiological processes, including the control of fat storage and metabolic status which are linked to food intake, energy homeostasis, and reproduction in all vertebrates. In the present study, we hypothesize that leptin is also present in various organs of the fresh water prawns, Macrobrachium rosenbergii. The existence and distribution of a leptin-like peptide in prawn tissues were verified by using Western blotting (WB) and immunohistochemical detection (ID) using primary antibody against human leptin...
June 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28622884/the-effect-of-caloric-restriction-on-the-forelimb-skeletal-muscle-fibers-of-the-hypertrophic-myostatin-null-mice
#20
Mohamed I Elashry, Antonios Matsakas, Sabine Wenisch, Stefan Arnhold, Ketan Patel
Skeletal muscle mass loss has a broad impact on body performance and physical activity. Muscle wasting occurs due to genetic mutation as in muscular dystrophy, age-related muscle loss (sarcopenia) as well as in chronic wasting disorders as in cancer cachexia. Food restriction reduces muscle mass underpinned by increased muscle protein break down. However the influence of dietary restriction on the morphometry and phenotype of forelimb muscles in a genetically modified myostatin null mice are not fully characterized...
June 13, 2017: Acta Histochemica
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