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Acta Histochemica

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https://www.readbyqxmd.com/read/30413282/the-efficacy-of-chronic-zinc-oxide-nanoparticles-using-on-testicular-damage-in-the-streptozotocin-induced-diabetic-rat-model
#1
Eman I El-Behery, Nesma I El-Naseery, Hanaa M El-Ghazali, Yaser H A Elewa, Eman A A Mahdy, Enas El-Hady, Mervat M H Konsowa
Testicular impairment is a common complication of Diabetes mellitus (DM). Zinc Oxide Nanoparticles (ZnO NPs) are a novel agent for Zn delivery with antidiabetic and antioxidant activities. However, few reports were recorded on it. The current study aimed to investigate the possible ameliorating effect of ZnO NPs treatment on testicular tissues alterations in streptozotocin (STZ)-induced diabetic rats. Therefore, thirty mature male Wistar rats were divided into three main groups: Control group (n = 18) was subdivided equally into three subgroups (negative control, vehicle and ZnO NPs), Diabetic group (n = 6) and ZnO NPs-treated diabetic group (n = 6)...
November 6, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30401477/amniotic-fluid-source-of-valuable-mesenchymal-stem-cells-and-alternatively-used-as-cryopreserved-solution
#2
Suteera Narakornsak, Sirinda Aungsuchawan, Peraphan Pothacharoen, Chaniporn Puaninta, Runchana Markmee, Waleephan Tancharoen, Tanongsak Laowanitwattana, Naree Poovachiranon, Chawapon Thaojamnong
Mesenchymal stem cells (MSCs), which possess remarkable capabilities, are found in amniotic fluid (AF). The findings of several studies have shown the potential benefits of these cells in applications of regenerative medicine. In clinical applications, an over-period of time is required in a preparation process that makes cell collection become more necessary. Herein, the aim of this study was to preserve and characterize the cell's properties after cell cryopreservation into an appropriate cryogenic medium...
November 3, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30401476/temporospatial-localization-of-telocytes-during-esophageal-morphogenesis-in-rabbit
#3
Dalia Ibrahim, Wafaa Gaber, Mahmoud Awad
Telocytes (TCs) are CD34 and Vimentin positive (+) immunoreactive stromal cells with a small-sized body and several extremely long telopodes. TCs have been described to provide a mechanical support throughout the tissue by making cellular connections (homo- or hetero) to form a 3D network. Such network can transmit the intercellular signaling. Recently, TCs have been described in the esophageal wall. However, information concerning the role of these cells in esophageal organization and development is rare. Thus, we aimed to record the temporo-spatial localization pattern of TCs during esophageal morphogenesis in rabbit...
November 3, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30392635/protective-effects-of-astaxanthin-on-subarachnoid-hemorrhage-induced-early-brain-injury-reduction-of-cerebral-vasospasm-and-improvement-of-neuron-survival-and-mitochondrial-function
#4
Yanbin Wang, Yao Liu, Yuchen Li, Binbing Liu, Pei Wu, Shancai Xu, Huaizhang Shi
The purpose of this study was to evaluate the neuroprotective effects of astaxanthin on early brain injury (EBI) caused by subarachnoid hemorrhage (SAH) in rats and to explore possible molecular mechanisms. Experimental SAH model was introduced in adult male SD rats by injecting autologous arterial blood into the prechiasmatic cistern. Astaxanthin (75 mg/kg bodyweight) or olive oil was administered by oral gavage at 3 h after SAH. Our results showed that astaxanthin attenuated SAH-induced cerebral vasospasm and reduced neuronal apoptosis...
November 1, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30389171/different-immunohistochemical-localization-for-tmem16a-and-cftr-in-acinar-and-ductal-cells-of-rat-major-salivary-glands-and-exocrine-pancreas
#5
Takuya Yokoyama, Masato Takemoto, Masato Hirakawa, Tomoyuki Saino
We investigated the mRNA expression and immunohistochemical localization of Cl- channels, transmembrane member 16A (TMEM16A or anoctamin 1), and cystic fibrosis transmembrane conductance regulator (CFTR) in rat major salivary glands and exocrine pancreas. RT-PCR detected mRNA expression of TMEM16A and CFTR in the extracts of the parotid gland (PG), submandibular gland (SMG), sublingual gland (SLG), and pancreas. Immunoreactivity for TMEM16A was localized in the apical membrane of serous acinar and intercalated ductal cells in the PG and SMG as well as mucous acinar cells in the SLG; however, it was not detected in striated ductal cells of these tissues...
October 30, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30348450/correlation-between-pancreatic-mast-cells-and-the-low-grade-inflammation-in-adipose-tissue-of-experimental-prediabetes
#6
Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany
The role of mast cells (MCs) in prediabetes (Pre-DM) is not clearly elucidated. In the current study rats (n = 22 each) were divided equally into; control and Pre-DM (received high fat diet, HFD) groups. Samples from pancreas as well as from visceral adipose tissue (VAT) were studied for the consequent changes. We detected a significantly increased mast cell count (MCC) in the pancreas of Pre-DM compared to that of control. Frequent degranulation of MC granules was observed in Pre-DM. VAT of the Pre-DM had significantly increased (p < 0...
October 19, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30342863/morphofunctional-parameters-of-rat-somatotrophes-after-acute-and-repeated-immobilization-or-restraint-stress
#7
Svetlana Trifunović, Iva Lakić, Predrag Vujović, Tanja Jevdjović, Branka Šošić-Jurjević, Verica Milošević, Jelena Djordjević
It is well known that stress changes levels of pituitary hormones in the bloodstream and in the pituitary itself. However, almost nothing is known about the impact of stress on histological and stereological parameters of the growth hormone producing cells (somatotrophs-GH cells). The aim of the present study was to investigate the effect of: acute and repeated immobilization; acute and repeated restraint on histological and morphofunctional parameters of somatotrophs in adult Wistar rats. Changes in the pituitary gland volume; the volume density and volume of somatotrophs following acute and repeated immobilization (IMO, R-IMO); acute and repeated restraint (R, R-R) were evaluated using a stereological system (newCAST), while growth hormone level within pituitary was determined by Western blot...
October 17, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30336951/hypertension-induced-changes-in-the-rat-myocardium-during-the-development-of-cardiac-hypertrophy-a-comparison-between-the-left-and-the-right-ventricle
#8
Alexandar Iliev, Georgi Kotov, Iva N Dimitrova, Boycho Landzhov
The hypertrophy of the cardiac muscle is one of the most significant maladaptive mechanisms activated in response to increased workload. It is associated with histological and ultrastructural alterations, changes in the quantitative parameters and the expression of different enzymes. While the structural and functional consequences of systemic hypertension on the left ventricle have been well evaluated, the right ventricle has received less attention. The aim of the present study was to analyse and compare the changes in the left and right ventricle during the development of cardiac hypertrophy initiated by systemic hypertension in different age groups of spontaneously hypertensive rats...
October 15, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30322809/luminescence-control-of-stomiidae-photophores
#9
Jérôme Mallefet, Laurent Duchatelet, Claire Hermans, Fernand Baguet
Nervous control of light emission from deep-sea mesopelagic fishes has been documented for several species. Studies on the nervous control of photophores from deep-sea luminescent fish, are mainly restricted to a pharmacological approach. For example, the light organs, called photophores, isolated from Argyropelecus hemygimnus and Maurolicus muelleri show a much higher sensitivity to adrenaline than to noradrenaline. According to these results and other information in different species, catecholamines are considered as main neurotransmitters triggering bioluminescence in deep-sea fishes...
October 12, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30318347/muscle-fibre-type-composition-in-the-lateral-muscle-of-olive-flounder-paralichthys-olivaceus
#10
Shuang Jiao, Xungang Tan, Yulei Sui, Feng You
In this paper, a combined-method study has been made on the lateral muscle of the teleost olive flounder Paralichthys olivaceus in just-hatched and adult stages. In just-hatched stage, both slow and fast muscle fibres were detected: (1) in situ hybridization analysis indicated that slow and fast myosin heavy chain genes were specifically expressed in the superficial and deep part of the myotomal muscle, respectively; (2) immunohistochemistry analysis showed that fibres in the deep part reacted with anti-fast myosin antibody F310; (3) western blot analysis detected a weak expression of slow myosin and a strong expression of fast myosin...
October 11, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30292321/histological-investigations-on-the-dura-mater-vascular-system-of-mice
#11
Badreddine Mecheri, Frank Paris, Hermann Lübbert
The human dura mater encephali is a well innervated and vascularized membrane. Its vascular system plays a crucial role in disorders and pathological cases like dural hematoma, meningitis, and different headache types. To investigate these diseases mouse models are increasingly being used. However, the literature on the vascular system of the mouse dura mater is sparse and explicit studies concerned exclusively with its vasculature are lacking. Here we present a detailed light and scanning electron microscopic investigation of the supratentorial dura mater of the mouse species, with a focus on the largest part of it, the parietal dura mater...
October 3, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30278995/differential-expression-of-mtor-signaling-pathway-proteins-in-lichen-planopilaris-and-frontal-fibrosing-alopecia
#12
Ozlem Dicle, Ciler Celik-Ozenci, Pinar Sahin, Eva Katharina Barbosa Pfannes, Annika Vogt, Berna Nazlim Altinok, Ulrike Blume-Peytavi
Dysregulation of the mammalian target of rapamycin (mTOR) signaling pathway has a variety of effects on the immune system and stem cell proliferation. Lichen planopilaris (LPP) and frontal fibrosing alopecia (FFA) are inflammatory scalp conditions resulting in permanent alopecia, which are thought to be related to stem cell damage. Here we investigate the expression of mTOR signaling pathway proteins in human hair follicles of LPP and FFA patients. The expression of mTOR pathway proteins in biopsy specimens from lesional and non-lesional scalp areas of eight LPP and five FFA patients were compared to control scalp biopsies from patients undergoing surgical excisions of sebaceous cysts...
September 29, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30268437/potential-therapeutic-role-of-melatonin-on-stz-induced-diabetic-central-neuropathy-a-biochemical-histopathological-immunohistochemical-and-ultrastructural-study
#13
Mohamed M M Metwally, Lamiaa L M Ebraheim, Azza A A Galal
The aim of the present study was to assess the therapeutic potential of melatonin (Mel) in diabetic central neuropathy in a rat model of streptozotocin (STZ)-induced diabetes. The rats were injected with 60 mg/kg STZ and diabetes was confirmed by blood glucose levels (BGL) ≥ 250 mg/dL. Mel treatment (50 mg/kg) was started 72 h before the STZ injection and continued for 45 days. In addition, normal control, vehicle (5% ethanol) control, and Mel-treated non-diabetic control were also included. STZ induced a diabetic phenotype with persistent hyperglycemia and elevated oxidative stress in the brain, liver, and kidneys compared to the control groups...
September 26, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30241677/expression-pattern-of-galectin-1-and-galectin-3-in-rat-testes-and-epididymis-during-postnatal-development
#14
Mehmet Özbek, Mustafa Hitit, Nuh Yıldırım, Özge Özgenç, Emel Ergün, Levent Ergün, Feyzullah Beyaz, Nevin Kurtdede, Hikmet Altunay
Galectins are a family of lectins-binding beta-galactosides involved in a variety of extracellular and intracellular processes, thereby contributing to homeostasis, cell adhesion, cellular turnover, and immunity. This study aimed to determine the localization and expression of galectin-1 (Gal-1) and galectin-3 (Gal-3) in the testis and epididymis of rats at postnatal [(prepubertal (day 5), pubertal (day 20), postpubertal (day 50) and mature (day 70)] periods by using immunohistochemistry and Western blotting...
September 18, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30236832/immunohistochemical-analysis-of-the-development-of-olfactory-organs-in-two-species-of-turtles-pelodiscus-sinensis-and-mauremys-reevesii
#15
Shoko Nakamuta, Satoshi Kusuda, Makoto Yokosuka, Kazumi Taniguchi, Yoshio Yamamoto, Nobuaki Nakamuta
The nasal cavity of turtles is composed of the upper and lower chambers, lined by the upper and lower chamber epithelia, respectively. In many turtles including the Reeve's turtle Mauremys reevesii, the upper chamber epithelium contains ciliated olfactory receptor neurons (ORNs) and the lower chamber epithelium contains microvillous ORNs. However, in the olfactory organ of the Chinese soft-shelled turtle Pelodiscus sinensis, both the upper and lower chamber epithelia contain ciliated ORNs. In the present study, we immunohistochemically examined the developmental process of olfactory organs in soft-shelled turtle and the Reeve's turtle to clarify the developmental origins of the lower chamber epithelium in these turtles...
September 17, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30224246/a-simple-and-efficient-toolset-for-analysing-mitochondrial-trafficking-in-neuronal-cells
#16
Victor A Shahen, Laurence C Cantrill, Nasim Bahram Sangani, John Christodoulou, Wendy A Gold
Mitochondria are crucial for cells, supplying up to 90% of the energy requirements for neurons. Their correct localisation is crucial and ensured by a transport system. Mitochondrial trafficking in neurons is particularly critical, because mitochondria must leave the soma and travel along the axon and dendritic network to facilitate neuronal function. Abnormal mitochondrial trafficking has been reported in several neurological disorders, therefore the ability to quantify and analyse mitochondrial trafficking is vital to improving our understanding of their pathogenesis...
September 14, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30224245/cholecalciferol-in-ethanol-preferring-rats-muscle-fibers-increases-the-number-and-area-of-type-ii-fibers
#17
Carina Guidi Pinto, Kátia Colombo Marchi, Ailton Amarante Arizza, Ana Paula Silveira Leite, Carlos Renato Tirapelli, Selma Maria Michelin Matheus
The chronic use of ethanol causes neuropathy and atrophy of type II fibers and promotes vitamin D decrease. This study evaluated cholecalciferol effects on the deep fibular nerve and extensor digitorum longus (EDL) muscle using an UChB ethanol-preferring rats model. Blood analyses were carried out to measure levels of 25-hydroxycholecalciferol (25(OH)D), calcium (Ca2+ ), Phosphorus (P), and parathyroid hormone (PTH). It was used EDL muscle to evaluate oxidative stress. The deep fibular nerve and EDL muscle were used for morphologic and morphometric assessment...
September 14, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30224244/effect-of-formalin-fixation-on-pcr-amplification-of-dna-isolated-from-healthy-autopsy-tissues
#18
Katarina Vitošević, Miloš Todorović, Tatjana Varljen, Živana Slović, Stevan Matić, Danijela Todorović
The aim of this study is to investigate the effects of formalin fixation on the degradation of DNA molecules in five different healthy tissues exempted during the autopsy, as well as the selection of the method that is most suitable for the DNA isolation. Heart muscle, liver, brain, lung and kidney tissue obtained from the healthy people who suddenly died from a violent death were used. The parts of tissue were fixed in 10% phosphate-buffered formalin as well as in 4% unbuffered formalin at room temperature...
September 14, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30219242/control-of-air-breathing-in-fishes-central-and-peripheral-receptors
#19
Luiz Henrique Florindo, Vinicius Araújo Armelin, David John McKenzie, Francisco Tadeu Rantin
This review considers the environmental and systemic factors that can stimulate air-breathing responses in fishes with bimodal respiration, and how these may be controlled by peripheral and central chemoreceptors. The systemic factors that stimulate air-breathing in fishes are usually related to conditions that increase the O2 demand of these animals (e.g. physical exercise, digestion and increased temperature), while the environmental factors are usually related to conditions that impair their capacity to meet this demand (e...
September 12, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30217408/expression-profile-of-some-neuronal-and-glial-cell-markers-in-the-ovine-ileal-enteric-nervous-system-during-prenatal-development
#20
Mehmet Özbek, Mehmet Fatih Bozkurt, Feyzullah Beyaz, Emel Ergün, Levent Ergün
The enteric nervous system (ENS) is a network of neurons and glia found in the gut wall and governs this gastrointestinal function independently from the central nervous system (CNS). ENS comprises the myenteric plexus (MP) and the submucous plexus (SP). In this study, we examined the expression profile of neurofilament heavy chain (NF-H), neuron-specific enolase (NSE), calcyclin (S100A6), vimentin and glial fibril acidic protein (GFAP) in ovine ileal enteric neurons and enteric glia cells (EGCs) during prenatal development using an immunohistochemical method...
September 11, 2018: Acta Histochemica
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