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Acta Cytologica

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https://www.readbyqxmd.com/read/29339640/application-of-immunocytochemistry-on-cell-block-sections-for-the-investigation-of-thyroid-lesions
#1
Niki Margari, Ilias Giovannopoulos, Abraham Pouliakis, Emmanouil Mastorakis, Alina Roxani Gouloumi, Ioannis G Panayiotides, Petros Karakitsos
OBJECTIVE: To investigate the potential of Classification and Regression Trees (CARTs) for the diagnosis of thyroid lesions based on cell block immunocytochemistry and cytological outcome. STUDY DESIGN: A total of 956 histologically confirmed cases (673 benign and 283 malignant) from patients with thyroid nodules were prepared via liquid-based cytology and evaluated; 4 additional slides were stained for cytokeratin 19 (CK-19), galectin 3 (Gal-3), Hector Battifora mesothelial cell 1 (HBME-1), and thyroglobulin...
January 16, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/29332062/cytologic-features-of-malignant-melanoma-with-osteoclast-like-giant-cells
#2
José A Jiménez-Heffernan, Magdalena Adrados, Patricia Muñoz-Hernández, Paloma Fernández-Rico, Ana I Ballesteros-García, Javier Fraga
BACKGROUND: Malignant melanoma showing numerous osteoclast-like giant cells (OGCs) is an uncommon morphologic phenomenon, rarely mentioned in the cytologic literature. The few reported cases seem to have an aggressive clinical behavior. Although most findings support monocyte/macrophage differentiation, the exact nature of OGCs is not clear. CASE: A 57-year-old woman presented with an inguinal lymphadenopathy. Sixteen years before, cutaneous malignant melanoma of the lower limb had been excised...
January 13, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/29320776/v600e-braf-versus-non-v600e-braf-mutated-lung-adenocarcinomas-cytomorphology-histology-coexistence-of-other-driver-mutations-and-patient-characteristics
#3
Kevan J Salimian, Roghayeh Fazeli, Gang Zheng, David Ettinger, Zahra Maleki
OBJECTIVES: We analyzed the morphologic features and clinical characteristics of lung adenocarcinomas (ACAs) harboring mutated BRAF. STUDY DESIGN: A review of the histology/cytology of BRAF-mutated lung ACAs was performed at the Johns Hopkins Hospital from January 1, 2013, to January 1, 2015. Patient demographics, clinical history, and ACA morphology were assessed. RESULTS: Thirty-six cases were identified with a median age of 66 years (range 44-87), 58% (21/36) were female, and 94% (34/36) were current or former smokers...
January 11, 2018: Acta Cytologica
https://www.readbyqxmd.com/read/29275417/cytomorphological-findings-of-follicular-dendritic-cell-sarcoma-on-fine-needle-aspiration-cytology
#4
Sandeep S Ojha, Rubal Jain, Farah Meenai, Ramrao Nilkanthe, Amit Haritwal
BACKGROUND: Follicular dendritic cell sarcoma (FDCS) is a rare neoplasm arising from follicular dendritic cells of germinal centers. The most common site of origin is lymph nodes and it may mimic a variety of tumors at that location, including carcinomas and sarcomas. Diagnosis is frequently missed on cytology as there are very few case reports describing the cytological characteristics of the lesion. Even on histology, a high degree of suspicion is required for an appropriate diagnosis...
December 23, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29183021/digital-applications-in-cytopathology-problems-rationalizations-and-alternative-approaches
#5
Nikolaos Chantziantoniou, Maheswari Mukherjee, Amber D Donnelly, Liron Pantanowitz, R Marshall Austin
OBJECTIVE: The aim of this work was to raise awareness of problems using digital applications for examining, teaching, and applying telecytology at King Abdulaziz Medical City (KAMC), Riyadh, Saudi Arabia; University of Nebraska Medical Center (UNMC), Omaha, NE, USA; and University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, USA. The objective was to rationalize problems and propose alternative digital approaches. STUDY DESIGN: We sought to identify solutions to improve the following: (a) interpretive examination scores at KAMC for complex cytological templates (i...
November 29, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29151086/analysis-of-the-cytomorphological-features-in-atypical-urine-specimens-following-application-of-the-paris-system-for-reporting-urinary-cytology
#6
Ryan Glass, Lisa Rosen, Karen Chau, Sylvat Sheikh-Fayyaz, Peter Farmer, Constantinos Coutsouvelis, Farah Slim, Ryan Brenkert, Kasturi Das, Stephen Raab, Rubina Cocker
BACKGROUND: This study investigates the use of The Paris System (TPS) for Reporting Urinary Cytopathology and examines the performance of individual and combined morphological features in atypical urine cytologies. METHODS: We reviewed 118 atypical cytologies with subsequent bladder biopsies for the presence of several morphological features and reclassified them into Paris System categories. The sensitivity and specificity of individual and combined features were calculated along with the risk of malignancy...
November 18, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29136629/modified-alcohol-formalin-cell-block-technique-in-head-and-neck-pathology-diagnosis
#7
Karishma M Desai, Punnya V Angadi, Alka D Kale, Seema Hallikerimath
OBJECTIVES: Cell block preparation is a recognized technique in histopathological diagnosis. This technique aids in maintaining an intact architecture and reducing the diagnostic errors associated with fine-needle aspiration cytology. Numerous techniques have been put forth, but the need for an optimal technique for routine use in the laboratory still persists. The aim of our study was to establish a cell block technique which aids in the accurate diagnosis of head and neck pathology...
November 15, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29136626/comparison-of-visual-inspection-with-acetic-acid-and-the-pap-smear-for-cervical-cancer-screening
#8
Parul Sinha, Pritika Srivastava, Anand Srivastava
OBJECTIVE: To evaluate the efficacy of visual inspection with acetic acid (VIA) in the early detection of cervical cancer and compare it with the Pap smear. STUDY DESIGN: This was a cross-sectional study conducted over a period of 3 months. A total of 316 patients who attended the Gynecology Outpatient Department of Era's Lucknow Medical College and Hospital, fulfilled the inclusion criteria, and gave their informed consent were included. RESULTS: VIA was found positive in 38 patients (12...
November 15, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29136620/use-of-automated-analysis-of-cervical-cytology-for-the-detection-of-high-grade-squamous-intraepithelial-lesions-in-human-papillomavirus-positive-women-a-future-practice
#9
Pierre Vassilakos, Rosa Catarino, Patrick Petignat
No abstract text is available yet for this article.
November 15, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29130942/high-grade-urothelial-carcinoma-on-urine-cytology-resembling-umbrella-cells
#10
Andrew A Renshaw, Edwin W Gould
CONTEXT: High-grade urothelial carcinoma (UC) cells have many appearances on urine cytology, but according to The Paris System, they can be easily distinguished from umbrella cells. OBJECTIVE: We aimed to define the incidence and appearance of high-grade UC cells that resemble umbrella cells in Cytospin preparations on urine cytology. RESULTS: Cytospin preparations from 331 cases with biopsy follow-up (230 benign/low-grade and 101 malignant [22 carcinoma in situ, 52 papillary, 19 invasive UC, 8 other] cases) were reviewed...
November 11, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29130974/atypical-squamous-cells-in-liquid-based-cervical-cytology-microbiology-inflammatory-infiltrate-and-human-papillomavirus-dna-testing
#11
Geilson Gomes de Oliveira, Renata Mirian Nunes Eleutério, Ana Katherine Silveira Gonçalves, Paulo César Giraldo, José Eleutério
OBJECTIVE: The aim of this study was to assess the correlation between atypical squamous cells (ASC) and inflammatory infiltrate and vaginal microbiota using cervical liquid-based cytological (SurePath®) and high-risk human papillomavirus (HR-HPV) tests. STUDY DESIGN: A cross-sectional study was conducted using a 6-year database from a laboratory in Fortaleza (Brazil). Files from 1,346 ASC cases were divided into subgroups and results concerning inflammation and vaginal microorganisms diagnosed by cytology were compared with HR-HPV test results...
November 8, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29130950/characteristics-of-false-negative-thyroid-fine-needle-aspirates
#12
Andrew A Renshaw, Edwin W Gould
INTRODUCTION: False-negative thyroid fine-needle aspirates (FNA) are not well characterized. METHODS: We correlated the results of all thyroid aspirations from 1997 to 2016 with histologic follow-up. RESULTS: There were 13,733 aspirates, 2,112 (15.3%) resections, and 678 malignancies (32.1%). Eighteen (2.7%) false-negative cases were identified (interpretation, n = 6; sampling, n = 7; and new nodules, n = 5). Interpretive false-negative cases were significantly less likely when the indeterminate rate was greater than 13% (p = 0...
November 4, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29073594/gaining-an-accurate-diagnosis-a-multidisciplinary-approach
#13
Irina Shabalova
No abstract text is available yet for this article.
October 27, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29069645/organized-cervical-cancer-screening-program-in-barretos-brazil-experience-in-18-municipalities-of-s%C3%A3-o-paulo-state
#14
Júlio César Possati-Resende, Fabiana de Lima Vazquez, Sinval Tadeu Biot, Edmundo Carvalho Mauad, Thais Talarico, José Humberto Tavares Guerreiro Fregnani, Adhemar Longatto-Filho
OBJECTIVES: The aim of this report is to demonstrate the Barretos Cancer Hospital initiative of organizational, laboratorial, and human resources training in the implementation of an organized cervical screening program in low-resource settings. METHODS: We developed a computational program to report all epidemiological, clinical, and laboratorial findings, and to trace all necessary information to recruit women for regular screening or for referral for complementary exams after liquid-based Pap test analyses...
October 26, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/29301126/cytology-formation-a-neutrophilic-happy-new-year
#15
Dima F Yassin, Mousa A Al-Abbadi
No abstract text is available yet for this article.
2018: Acta Cytologica
https://www.readbyqxmd.com/read/28977789/intraoperative-squash-and-touch-preparation-cytology-of-brain-lesions-stained-with-h-e-and-diff-quik%C3%A2-a-20-year-retrospective-analysis-and-comparative-literature-review
#16
Makoto Hamasaki, Karen H F Chang, Kazuki Nabeshima, Pamela S Tauchi-Nishi
OBJECTIVE: Squash preparation (SP) is a rapid technique for the intraoperative assessment of brain lesions. Only a few studies have employed touch preparation (TP) cytology and Diff-QuikTM (DQ) staining in conjunction with SP. Our study aimed to assess the diagnostic efficacy of SP of brain lesions at our institution, ascertain the additional effect of TP and DQ staining, examine factors affecting the sensitivity and specificity of our methods, and compare our findings with those of previous investigations...
October 5, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28954257/a-modified-direct-smear-processing-technique-employing-two-step-centrifugation-fixation-is-useful-for-detecting-high-grade-urothelial-carcinoma
#17
Yasuhiro Toyonaga, Kazuto Yamazaki, Yoshinori Koyama, Masatoshi Yamada, Yasuo Ishida
OBJECTIVE: To demonstrate the usefulness of a direct-smear processing technique employing two-step centrifugation/fixation processing (TSCFP) in the cytoscreening of high-grade urothelial carcinoma (HGUC). STUDY DESIGN: Using the T24 HGUC cell line, we compared the cell yield and the morphological preservation of preparations concurrently processed by direct-smear, SurePath, ThinPrep, and TSCFP techniques. A total of 287 urine cytology cases subjected to TSCFP over a period of 6 years were reviewed and reclassified according to the Paris System for Reporting Urinary Cytology (PSRUC) and correlated with histology results...
September 28, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28946148/assessment-of-clinically-suspected-tubercular-lymphadenopathy-by-real-time-pcr-compared-to-non-molecular-methods-on-lymph-node-aspirates
#18
Vivek Gupta, Arvind Bhake
BACKGROUND: The diagnosis of tubercular lymphadenitis (TBLN) is challenging. This study assesses the role of diagnostic intervention with real-time PCR in clinically suspected tubercular lymphadenopathy in relation to cytology and microbiological methods. METHODS: The cross-sectional study involved 214 patients, and PCR, cytology, and Ziehl-Neelsen (ZN) staining was performed on aspirates. The findings were compared with culture on Lowenstein-Jensen medium. The overall concordance of cytology and PCR, both individually and combined, was calculated...
September 26, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28848083/use-of-exfoliative-specimens-and-fine-needle-aspiration-smears-for-mutation-testing-in-lung-adenocarcinoma
#19
Deepali Jain, Vijayakumar S Ramachandrappa, Varsha Singh, Prabhat S Malik, Karan Madan, Mohammed Faruq, Randeep Guleria
OBJECTIVE: Cytology specimens are considered to be a promising alternative for detecting driver mutations in lung cancer patients. We aimed to explore the suitability and utility of various cytology samples of non-small-cell lung cancer (NSCLC) patients for mutation testing. In addition to mutation detection, the importance of preanalytic factors was discussed. DESIGN: A total of 116 cytology samples including 32 controls comprising pleural effusions, bronchial washings, and direct fine-needle aspiration (FNA) smears were included in the study for the detection of EGFR, KRAS, and Her-2/neu gene mutations...
August 22, 2017: Acta Cytologica
https://www.readbyqxmd.com/read/28746917/cytological-features-of-the-large-cell-variant-of-small-cell-ovarian-carcinoma-in-young-patients-with-hypercalcemia-histological-findings-and-review-of-the-literature
#20
Helen J Trichia, Paraskevi Tziakou, Dimitrios C Papatheodorou, Joanna Lekka
OBJECTIVE: To present the cytological features of a very rare and lethal ovarian neoplasm occurring in the young. STUDY DESIGN: We reviewed the cytological findings as they presented in touch imprints obtained from an ovarian mass sent to our department for frozen section investigation. RESULTS: Smears were highly cellular. The cells were of intermediate size with a moderate amount of microvacuolated, pale, or eosinophilic cytoplasm with indistinct cell borders...
July 27, 2017: Acta Cytologica
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