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Connective Tissue Research

Chunming Xu, Yanjun Zhang, Linawati Sutrisno, Li Yang, Rongfu Chen, K L Paul Sung
PURPOSES: To investigate the ability of synoviocytes (SCs) in regulating MMPs expression in the posterior cruciate ligament fibroblasts (PCLfs) after TNF-α treatment, to test whether a specific inflammation inhibitor Bay11-7082 can antagonize the expression of MMPs in PCLfs after injury. METHODS: The microenvironment of knee joint cavity after PCL injury was mimicked in an in vitro co-culture system. The effects of TNF-α treatment on the expression of MMPs in PCL fibroblasts (PCLfs) were studied...
October 29, 2018: Connective Tissue Research
Gioacchino Conoscenti, Francesco Carfì Pavia, Alfred Ongaro, Valerio Brucato, Clemens Goegele, Silke Schwarz, Aldo R Boccaccini, Katharina Stoelzel, Vincenzo La Carrubba, Gundula Schulze-Tanzil
Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity. Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair. Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis. The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes...
October 22, 2018: Connective Tissue Research
Willy Roque, Ross Summer, Freddy Romero
Idiopathic pulmonary fibrosis (IPF) is an extremely aggressive lung disease that develops almost exclusively in older individuals, carries a very poor prognosis, and lacks any truly effective therapies. The current conceptual model is that IPF develops because of an age-related decline in the ability of the lung epithelium to regenerate after injury, largely due to death or senescence of epithelial progenitor cells in the distal airways. This loss of regenerative capacity is thought to initiate a chronic and ineffective wound-healing response, characterized by persistent, low-grade lung inflammation and sustained production of collagen and other extracellular matrix materials...
October 22, 2018: Connective Tissue Research
Ryosuke Nakagawa, Ryuichiro Akagi, Satoshi Yamaguchi, Takahiro Enomoto, Yusuke Sato, Seiji Kimura, Yuya Ogawa, Aya Sadamasu, Seiji Ohtori, Takahisa Sasho
PURPOSE: Our aims were 1) to estimate the duration of short interfering RNA (siRNA) effect on matrix metalloproteinase-13 (Mmp-13) levels by a single intra-articular injection using a mouse knee osteoarthritis (OA) model and 2) to test whether repeated injections results in any additional suppressive effect on cartilage degradation compared to a single injection. MATERIALS AND METHODS: OA was induced in 9 weeks old male C57BL/6 mice by destabilization of medial meniscus (DMM)...
October 21, 2018: Connective Tissue Research
Jinyong Ju, Du Yu, Feng Xue, Yong Zhao, Weizhe Shi, Mingmang Pan, Guo Tang, Haijun Xiao
PURPOSE: To investigate the pathogenesis and find a better prophylactic method of acquired heterotopic ossification (HO). MATERIALS AND METHODS: In the first part, we designed the brain-traumatic/burn/tenotomy rat model and testified its efficacy as HO model. 44 rats were randomly divided into experimental group and control group. After operation, the bilateral tendons of 2 rats were collected at the 2nd , 3rd , 4th , 6th , 8th , and 10th weeks to determine the expression levels of p65...
October 10, 2018: Connective Tissue Research
Jie Zhou, Anjing Liang, Junmin Hong, Jianchao Sun, Xiaolin Lin, Yan Peng, Xiaofei Wang, Siguo Sun, Dong Xiao, Kang Xu, Wei Ye
AIM: miR-155 is a pro-inflammatory or anti-inflammatory factor depending on the cell type in which it is expressed. miR-155 controls apoptosis and matrix degradation in nucleus pulposus (NP) cells in vitro. The aim of this study is to explore the effect of miR-155 in vivo and further investigate the mechanism of miR-155 in vitro. METHODS: MRI, hematoxylin-eosin staining, or Collagen-II immunochemistry were performed to observe intervertebral disk degeneration in conditional miR-155 overexpression mice and miR-155 knockout mice...
October 4, 2018: Connective Tissue Research
Ye-Li Zhao, Xuan Zhang, Wei-Wei Liu, Yu-Ting Yang, Zhuo-Kun Gao, Xiao-Ling Liu, Wei Liu, Toshihiko Hayashi, Masayuki Yamato, Hitomi Fujisaki, Shunji Hattori, Kazunori Mizuno, Yuji Atsuzawa, Shin-Ichi Tashiro, Satoshi Onodera, Takashi Ikejima
PURPOSE: Our previous studies indicate that phorbol 12-myristate 13-acetate (PMA)-treated U937 cells cultured on collagen I -coated dishes express lowered production of pro-inflammatory mediators in parallel through reduced Reactive oxygen species (ROS) levels. By contrast, PMA-treated U937 cells on gelatin, the denatured collagen, show enhanced production of pro-inflammatory mediators, mediated by up-regulating autophagy levels. The present study is aimed to investigate the effect of ROS levels in PMA-treated U937 cells cultured on gelatin-coated surface...
October 2, 2018: Connective Tissue Research
Phong K Nguyen, Xuan Sabrina Pan, Jiewen Li, Catherine K Kuo
Tendon is a specialized connective tissue that connects muscle to bone, thereby enabling musculoskeletal movement. Tendon injury leads to formation of tissue with aberrant functional properties. Current approaches to treat tendon injuries, including surgical repair and tissue engineering, have not achieved normal tendon. A roadmap of markers could help with identifying when mis-steps occur during aberrant tendon formation and providing instructions for normal tendon formation. We propose this roadmap should be based on the embryo-the perfect model of tissue formation...
September 20, 2018: Connective Tissue Research
Rachel Choi, Margaret Smith, Elizabeth Clarke, Christopher Little
PURPOSE: Summarise available evidence comparing the cellular, biochemical, structural and biomechanical properties, and the changes that occur in these parameters in response to stimuli, in differentially loaded tendons across different stages of life. METHODS: The PubMed database was searched for literature pertaining to differences between tendons using the term "tendon" or "tendinopathy", plus one or more of the following descriptors: "loading", "positional", "weight- or load-bearing", and "energy-storing"...
September 20, 2018: Connective Tissue Research
Katarzyna Gawron, Katarzyna Łazarz-Bartyzel, Anna Kowalska, Grzegorz Bereta, Zuzanna Nowakowska, Paweł Plakwicz, Jan Potempa, Andrzej Fertala, Maria Chomyszyn-Gajewska
PURPOSE: Investigate the content of fibrotic fibrils in gingival tissue and the proliferation of fibroblasts collected from recurrent and non-recurrent hereditary gingival fibromatosis (HGF) and idiopathic gingival fibromatosis (IGF). METHODS: Gingival biopsies were collected from HGF (n = 3) and IGF (n = 3) donors with recurrent and non-recurrent gingival overgrowths and from a control group (Ctrl, n = 3). Hematoxylin staining was performed to evaluate the histomorphology of gingival tissue...
September 20, 2018: Connective Tissue Research
Antonia F Chen, Yong Seuk Lee, Adam J Seidl, Joseph A Abboud
Large joint arthrofibrosis and scarring, involving the shoulder, elbow, hip, and knee, can result in the loss of function and immobility. The pathway of joint contracture formation is still being elucidated and is due to aberrations in collagen synthesis and misorientation of collagen fibrils. Novel antibodies are being developed to prevent arthrofibrosis, and current treatment methods for arthrofibrosis include medical, physical, and surgical treatments. This article describes the biology of joint contracture formation, along with current and future pharmacologic, biologic, and medical interventions...
September 20, 2018: Connective Tissue Research
Jack G Graham, Mark L Wang, Michael Rivlin, Pedro K Beredjiklian
Tendon injuries of the hand that require surgical repair often heal with excess scarring and adhesions to adjacent tissues. This can compromise the natural gliding mechanics of the flexor tendons in particular, which operate within a fibro-osseous tunnel system similar to a set of pulleys. Even combining the finest suture repair techniques with optimal hand therapy protocols cannot ensure predictable restoration of hand function in these cases. To date, the majority of research regarding tendon injuries has revolved around the mechanical aspects of the surgical repair (i...
September 7, 2018: Connective Tissue Research
Mark L Wang, Michael Rivlin, Jack G Graham, Pedro K Beredjiklian
Peripheral nerve injuries (PNI) resulting from trauma can be severe and permanently debilitating. Despite the armamentarium of meticulous microsurgical repair techniques that includes direct repair, grafting of defects with autograft nerve, and grafting with cadaveric allografts, approximately one-third of all PNI demonstrate incomplete recovery with poor restoration of function. This may include total loss or incomplete recovery of motor and/or sensory function, chronic pain, muscle atrophy, and profound weakness, which can result in lifelong morbidity...
September 6, 2018: Connective Tissue Research
Coralee E Tye, Joseph R Boyd, Natalie A Page, Michelle M Falcone, Janet L Stein, Gary S Stein, Jane B Lian
Long noncoding RNAs (lncRNAs) have recently emerged as novel regulators of lineage commitment, differentiation, development, viability, and disease progression. Few studies have examined their role in osteogenesis; however, given their critical and wide-ranging roles in other tissues, lncRNAs are most likely vital regulators of osteogenesis. In this study, we extensively characterized lncRNA expression in mesenchymal cells during commitment and differentiation to the osteoblast lineage using a whole transcriptome sequencing approach (RNA-Seq)...
December 2018: Connective Tissue Research
Pascal R Buenzli, Chloé Lerebours, Andreas Roschger, Paul Roschger, Richard Weinkamer
PURPOSE: Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mineralization by simulating BMDDs in low-turnover conditions. MATERIALS AND METHODS: An established computational model of the BMDD that accounts for mineralization and remodeling processes was extended to limit mineralization to various maximum calcium capacities of bone...
December 2018: Connective Tissue Research
Ceren Tuzmen, Phil G Campbell
AIM OF THE STUDY: The peripheral nervous system is involved in regulation of bone metabolism via sensory and sympathetic innervation. Substance P (SP) and calcitonin gene-related peptide (CGRP) are two sensory neuropeptides that have been associated with regulation of osteogenic differentiation. However, the interaction between SP and CGRP both with each other and the bone morphogenetic protein 2 (BMP2) in regulation of osteogenic differentiation has not been studied. Therefore, the aim of this study was to investigate the interaction between SP and CGRP on BMP2-induced bone differentiation using model progenitor cells...
December 2018: Connective Tissue Research
Scott V Wiener, Ling Chen, Alex R Shimotake, Misun Kang, Marshall L Stoller, Sunita P Ho
Purpose/Aim: The most common kidney stone composed of calcium oxalate forms on interstitial calcium phosphate mineral known as a Randall's plaque (RP). Due to limited information about events leading to the initial deposition of nanometer size interstitial calcium phosphate pre-clusters, there continues to be a debate on the initial site of calcium phosphate deposition and factors leading to stone formation. MATERIALS AND METHODS: High-resolution X-ray micro-computed tomography (CT), and light and electron microscopy techniques were used to characterize human renal pyramids and five representative kidney stones with identifiable stems...
December 2018: Connective Tissue Research
Benjamin R Coyac, Guillaume Falgayrac, Guillaume Penel, Alain Schmitt, Thorsten Schinke, Agnès Linglart, Marc D McKee, Catherine Chaussain, Claire Bardet
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process...
December 2018: Connective Tissue Research
Keith Alvares, Caroline J DeHart, Paul M Thomas, Neil L Kelleher, Arthur Veis
BACKGROUND: Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. MATERIALS AND METHODS: The tooth proteins were extracted and the phosphoproteins occluded in the mineral were enriched by passage through a ProQ Diamond phosphoprotein enrichment column, and subjected to MS/MS analysis...
December 2018: Connective Tissue Research
Fleur Berès, Guilhem Lignon, Stéphan Rouzière, Cédric Mauprivez, Stéphane Simon, Ariane Berdal, Arnaud Dessombz
No abstract text is available yet for this article.
December 2018: Connective Tissue Research
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