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Connective Tissue Research

Qingshen Wei, Xiangwei Zhang, Caiju Zhou, Qiang Ren, Yuntao Zhang
Degeneration of the intervertebral discs, a natural progression of the aging process, is strongly implicated as a cause of low back pain. Aggrecan is the major structural proteoglycan in the extracellular matrix of the intervertebral disc. It is large, possessing numerous glycosaminoglycan chains and the ability to form aggregates in association with hyaluronan. The negatively charged glycosaminoglycan side chains in aggrecan in the nucleus pulposus of the intervertebral discs can bind electrostatically to polar water molecules, which are crucial for maintaining the well-hydrated state that enables the discs to undergo reversible deformation under compressive loading...
July 11, 2018: Connective Tissue Research
Shubo Li, Yihuai Pan
BACKGROUND/AIMS: Connective tissue growth factor (CTGF) is a downstream mediator of transforming growth factor-beta 1 (TGF-β1 ) and TGF-β1 -induced CTGF expression is regulated through SMAD and mitogen-activated protein kinase (MAPK) signaling pathways. However, little is known about the localization of CTGF and TGF-β1 signaling cascades during incisor development. Therefore, we aimed to investigate the distribution pattern of TGF-β1 , CTGF, phosphorylated-SMAD2/3 (p-SMAD2/3) and phosphorylated-ERK1/2 (p-ERK1/2) in the developing mouse incisors...
July 10, 2018: Connective Tissue Research
Elisabeth A Lemmon, Ryan C Locke, Adrianna K Szostek, Elahe Ganji, Megan L Killian
PURPOSE: Identify the healing outcomes following a partial-width, full-thickness injury to the rotator cuff tendon-bone attachment and establish if the adult attachment can regenerate the morphology of the healthy attachment. HYPOTHESIS: We hypothesized that a partial-width injury to the attachment would heal via fibrosis and bone remodeling, resulting in increased cellularity and extra-cellular matrix deposition, reduced bone volume (BV), osteoclast presence, and decreased collagen organization compared to shams...
July 2, 2018: Connective Tissue Research
Yan Zhang, Mei Yu, Xueyong Zhao, Minjia Dai, Chang Chen, Weidong Tian
OBJECTIVES: Adherent culture which is used to collect adipose tissue extract (ATE) previously brings the problem of inhomogeneity and non-repeatability. Here we aim to extract ATE with stirred suspension culture to speed up the extraction process, stabilize the yield, and improve consistent potency metrics of ATE. MATERIALS AND METHODS: ATE was collected with adherent culture (ATE-A) and stirred suspension culture (ATE-S) separately. Protein yield and composition were detected by SDS-PAGE, while cytokines in ATE were determined with ELISA...
June 25, 2018: Connective Tissue Research
Michael D Kurdziel, Abigail Davidson, David Ross, Joseph Seta, Shashin Doshi, Kevin C Baker, Tristan Maerz
PURPOSE: The rat rotator cuff (RC) model is used to study RC pathology and potential treatment, however, native scar-mediated healing allows the rat RC to recover at 4-6 weeks but little is known about acute healing. This study characterized the properties of the repaired and non-repaired rat RC following surgical detachment. MATERIALS AND METHODS: Forty-eight rats underwent surgical RC detachment and received surgical repair (Repair) or left unrepaired (Defect)to either 12 or 19 days...
June 22, 2018: Connective Tissue Research
Vivianne Izabelle de Araújo Baptista, Hananiah Tardivo Quintana, Mariana Cruz Lazzarin, Izabelle Dias Benfato, Francine Pereira de Carvalho, Luciana Le Sueur Maluf, Camila Aparecida Machado de Oliveira, Josemberg da Silva Baptista, Flavia de Oliveira
Extensive burn may cause acute resistance to insulin, which accentuates hypermetabolism, impairs glucose metabolism, immune dysfunction and risks of sepsis. To minimize these effects, insulin is used as a treatment. The purpose was to analyze the collagen-elastic arrangement effects of insulin on the burned skin. Wistar rats were assigned in groups: control (C); control with insulin (C+I); scald burn injury (SBI) and SBI with insulin (SBI+I). SBI were submitted to 45% total body surface area burn and the insulin-treated groups received insulin (5 UI/Kg/day) for 4 or 14 days (d)...
June 21, 2018: Connective Tissue Research
Juan Paredes, David A Shiovitz, Nelly Andarawis-Puri
PURPOSE: Tendon tears are common injuries that heal with scar formation. Interestingly, MRL/MpJ mice heal without scar in several tissues, including tendon. Most hypotheses regarding scarless healing implicate the systemic environment. However, the tissue-specificity of this regenerative response and our previous findings showing regeneration of sub-rupture tendon injuries, which lack an overt systemic response, motivate a tissue-driven hypothesis. Our objective is to investigate the potential of the local tendon environment in driving scarless healing (1) by comparing the systemic response and the healing capacity associated with ear and tendon injuries in MRL/MpJ mice, and (2) by comparing intrinsic healing properties between MRL/MpJ and normal healer C57Bl/6 tendons...
June 21, 2018: Connective Tissue Research
Haixin Liu, Minsheng Yang, Guofeng Wu, Lianjun Yang, Yanlin Cao, Chun Liu, Zhiwen Tan, Yanglei Jin, Jiasong Guo, Lixin Zhu
In physiological and pathological environments, the concentration of oxygen around osteoblasts varies widely. No studies have systematically evaluated the effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of osteoblasts. In this study, we cultured the osteoblast precursor cell line MC3T3-E1 in small individual chambers with oxygen concentrations of 1%, 3%, 6%, 9%, and 21%. Cell proliferation was evaluated by the proliferation index test and EdU staining...
June 19, 2018: Connective Tissue Research
Serdar Yuksel, M Akif Guleç, Zeki Gultekin, Aysel Caglar, Ozan Beytemur, Ender Alagoz, A Akdogan Eker, Cansu Subaşı, Erdal Karaoz
PURPOSE: This study aimed to assess Achilles tendon repair in rats following splenectomy to simulate patients with musculoskeletal system injury who had splenectomy after spleen injury, a situation often seen in orthopedics and traumatology practice. MATERIALS AND METHODS: The study included 32 male Sprague-Dawley rats (10 months old; average weight, 394.5 ± 28.3 g). The rats were fed with standard rodent food ad libitum at 22°C in a dark environment for 12 h...
June 19, 2018: Connective Tissue Research
Mei Li, Chi Zhang, Xinhan Li, Zeheng Lv, Yao Chen, Jiyuan Zhao
AIM: Isoquercitrin is widely present in fruits, vegetables and medicinal herbs. As a natural phytoestrogen, isoquercitrin has been considered a possible osteoporosis prevention option to avoid the risk of hormone therapy. MATERIALS AND METHODS: The cell proliferation of osteoblasts and bone mesenchymal stem cells (BMSCs) was examined by cell counting kit-8 (CCK-8). The osteogenic differentiation was evaluated by real-time qPCR, ALP staining and Alizarin Red S staining...
June 18, 2018: Connective Tissue Research
Jeremy D Eekhoff, Fei Fang, Spencer P Lake
The hierarchical structure of tendon allows for attenuation of mechanical strain down decreasing length scales. While reorganization of collagen fibers accounts for microscale strain attenuation, cross-linking between collagen molecules contributes to deformation mechanisms at the fibrillar and molecular scales. Divalent and trivalent enzymatic cross-links form during the development of collagen fibrils through the enzymatic activity of lysyl oxidase (LOX). By establishing connections between telopeptidyl and triple-helical domains of adjacent molecules within collagen fibrils, these cross-links stiffen the fibrils by resisting intermolecular sliding...
June 6, 2018: Connective Tissue Research
Brianne K Connizzo, Alan J Grodzinsky
PURPOSE: Tendinopathy is a significant clinical problem thought to be associated with altered mechanical loading. Explant culture models allow researchers to alter mechanical loading in a controlled in vitro environment while maintaining tenocytes in their native matrix. However, current models do not accurately represent commonly injured tendons, ignoring contributions of associated musculature and bone, as well as regional collagen structure. This study details the characterization of amouse rotator cuff explant culture model, including bone, tendon, and muscle (BTM)...
June 6, 2018: Connective Tissue Research
Katie J Sikes, Jun Li, Shu-Guang Gao, Quan Shen, John D Sandy, Anna Plaas, Vincent M Wang
Purpose/Aim of the study: Healthy tendons are maintained in homeostasis through controlled usage of glucose for energy and redox equilibrium. Tendon cell stress imposed by overuse injury or vascular insufficiency is accompanied by activation of wound healing pathways which facilitate an adaptive response and the restoration of homeostasis. To understand this response at the gene expression level we have studied the in vivo effects of injected TGF-β1 in a murine model of tendinopathy, as well as treatment of murine tendon explants with either TGF-β1 or hypoxia in vitro...
June 6, 2018: Connective Tissue Research
Javier Fernández-Torres, Gabriela Angélica Martínez-Nava, Francesca Oliviero, Alberto Gabriel López-Reyes, Karina Martínez-Flores, Daniela Garrido-Rodríguez, Adriana Francisco-Balderas, Yessica Zamudio-Cuevas
Background The presence of genetic variants in the uric acid (UA) transporters can be associated with hyperuricemia, and therefore with an increased risk of monosodium urate (MSU) crystal precipitation. The inflammatory process triggered by these crystals lead to cartilage damage which, in turn, could promote knee osteoarthritis (KOA). Objective To determine whether genetic polymorphisms of the UA transporters and its interactions are associated with KOA. Materials and Methods Two hundred forty-three unrelated Mexican-mestizo individuals were recruited for this case control-study...
June 1, 2018: Connective Tissue Research
Jie Zhou, Anjing Liang, Junmin Hong, Jianchao Sun, Xiaolin Lin, Yan Peng, Xiaofei Wang, Siguo Sun, Dong Xiao, Kang Xu, Wei Ye
AIM: miR-155 is a pro-inflammatory or anti-inflammatory factor depending on the cell type in which it is expressed. miR-155 controls apoptosis and matrix degradation in NP cells in vitro. Does a similar phenomenon exist in vivo? Is there other mechanism except for the reports? The aim of this study is to explore the effect of miR-155 in vivo and further investigate the mechanism of miR-155 in vitro. METHODS: MRI, HE staining or collagen II immunochemistry were performed to observe intervertebral disc degeneration in conditional miR-155 overexpression mice and miR-155 knockout mice...
May 31, 2018: Connective Tissue Research
Yihua Song, Chenfei Wang, Zhifeng Gu, Peipei Cao, Dan Huang, Guijuan Feng, Min Lian, Ye Zhang, Xingmei Feng, Zhenran Gao
AIM: Casein kinase 2 interacting protein-1 (CKIP-1) is a recently discovered intracellular regulator of bone formation, muscle cell differentiation and tumor cell proliferation. Our study aims to identify the inhibition of BMP2-Smad1/5 signaling by CKIP-1 in odontoblastic differentiation of human dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs infected CKIP-1 siRNA or transfected CKIP-1 full-length plasmid were cultured in odontoblastic differentiation medium or added noggin (200 ng/mL) for 21 days...
May 31, 2018: Connective Tissue Research
Mahmut Cerkez Ergoren, Burcu Turkgenc, Kerem Teralı, Orhan Rodoplu, Aline Verstraeten, Lut Van Laer, Gamze Mocan, Bart Loeys, Omer Tetik, Sehime G Temel
Marfan syndrome (MFS) is a multi-systemic autosomal dominant condition caused by mutations in the gene (FBN1) coding for fibrillin-1. Mutations have been associated with a wide range of overlapping phenotypes. Here, we report on an extended family presenting with skeletal, ocular and cardiovascular clinical features. The 37-year-old male propositus, who had chest pain, dyspnea and shortness of breath, was first diagnosed based on the revised Ghent criteria and then subjected to molecular genetic analyses. FBN1 sequencing of the proband as well as available affected family members revealed the presence of a novel variant, c...
May 28, 2018: Connective Tissue Research
Lin Ye, Yuanyuan Yao, Hui Guo, Yun Peng
AIM OF THE STUDY: Blepharoptosis is a drooping of the upper eyelid, usually due to dysfunction of the levator palpebrae superioris (LPS). Recently, skeletal muscle satellite cells (SSCs) have been reported to promote the repair of damaged skeletal muscle. This study aims to investigate the potential contribution of exogenous SSCs to the regeneration of mechanically damaged LPS. MATERIALS AND METHODS: Thirty-two rats were randomly divided into four groups, including control group, SSCs-treated group, SSCs-treated injury group and non-treated injury group...
May 17, 2018: Connective Tissue Research
Shivam H Patel, Arman Sabbaghi, Chad C Carroll
OVERVIEW: Tendon collagen fibril degradation is commonly seen in tendons of diabetics, but the mechanisms responsible for these changes remain to be elucidated. We have demonstrated that streptozotocin (STZ)-induced diabetes increases tendon cell proliferation and collagen content. In the present study, we evaluated that impact of STZ-induced diabetes on mRNA transcripts involved with collagen fibril organization, extracellular matrix (ECM) remodeling, apoptosis, and proliferation. MATERIALS AND METHODS: Rats were divided into four groups: nondiabetic (control, n = 9), 1 week (acute, n = 8) or 10 weeks of diabetes (chronic, n = 7), and 10 weeks of diabetes with insulin (insulin, n = 8)...
May 10, 2018: Connective Tissue Research
Elisa Belluzzi, Eleonora Olivotto, Giovanna Toso, Augusto Cigolotti, Assunta Pozzuoli, Carlo Biz, Giovanni Trisolino, Pietro Ruggieri, Brunella Grigolo, Roberta Ramonda, Marta Favero
AIM: Osteoarthritis (OA) is a whole joint pathology involving cartilage, synovial membrane, meniscus, subchondral bone, and infrapatellar fat pad (IFP). Synovitis has been widely documented in OA suggesting its important role in pathogenesis. The aim of this study was to investigate the role of different joint tissues in promoting synovitis. MATERIALS AND METHODS: Conditioned media (CM) from cartilage, synovial membrane, meniscus, and IFP were generated from tissues of five patients undergoing total knee replacement and used to stimulate a human fibroblast-like synoviocytes cell line (K4IM)...
May 8, 2018: Connective Tissue Research
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