Read by QxMD icon Read

Connective Tissue Research

Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J Van Wijnen, Michael J Yaszemski, Sanjeev Kakar
PURPOSE: Revascularization of natural and synthetic scaffolds is a critical part of the scaffold's incorporation and tissue ingrowth. Our goals were to create a biocompatible polymer scaffold with 3D-printing technology, capable of sustaining vascularization and tissue ingrowth. METHODS: We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow tissue ingrowth via large interconnected pores. The scaffolds were prepared with Poly(lactic-co-glycolic acid)(PLGA) microspheres seeded with or without different growth factors including VEGF,FGF-2, and/or BMP-2...
March 7, 2018: Connective Tissue Research
Sandeep C Chaudhary, Sana Khalid, Victoria Smethurst, Daisy Monier, James Mobley, Alexis Huet, James F Conway, Dobrawa Napierala
Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from vascular smooth muscle cells significantly contribute to the initiation and progression of this pathology. Recently, we have demonstrated that elevated phosphate stimulates release of extracellular vesicles from osteogenic cells at the initiation of the mineralization process. Here, we used MOVAS cell line as an in vitro model of vascular calcification to examine whether vascular smooth muscle cells respond to high phosphate levels in a similar way and increase formation of extracellular vesicles...
February 22, 2018: Connective Tissue Research
Satish Kumar, Rakesh Kumar Majhi, Sridhar Sanyasi, Chandan Goswami, Luna Goswami
With increased life expectancy, disorders in life style and other clinical conditions, the changes in the connective tissues such as in bone impose a diverse biomedical problems. Such problems are expensive, more serious in old ages and are fatal in certain pathophysiological settings which demands functional and/or artificial bones prepared at low cost. Cells having osteogenic properties and/or belong to osteogenic lineages are extremely specific for their surface requirements. Therefore, suitable surfaces are the critical bottle neck for successful bone tissue engineering...
February 20, 2018: Connective Tissue Research
Xing Yang, Lei Wang, Zhang-Qin Yuan, Ping-Hui Zhou, Geng-Lei Chu, Bin Li, Jun-Ying Sun
The loss of intervertebral disc (IVD) cells due to excessive apoptosis is a central factor in the process of IVD degeneration. However, whether interleukin-1β (IL-1β) induces apoptosis through the extracellular signal-regulated kinase (ERK) pathway in annulus fibrosus cells (AFCs) has not been investigated. In this study, we observed increases in expression of Bax, caspase-3, and caspase-9, and a decrease in expression of Bcl-2 in patients with degenerative IVDs. In vitro, we found that IL-1β induced apoptosis and ERK pathway activation in AFCs...
February 18, 2018: Connective Tissue Research
Jian Zhang, Xinyu Qiu, Kedi Xi, Wentao Hu, Hailong Pei, Jing Nie, Ziyang Wang, Jiahan Ding, Peng Shang, Bingyan Li, Guangming Zhou
Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due to the affected behaviors of bone cells, including bone mesenchymal stem cells (BMSCs), osteoblasts and osteoclasts. BMSCs and osteoblasts are relatively radioresistant compared with osteoclasts and its progenitors...
February 15, 2018: Connective Tissue Research
Qi Wu, Jing Li, Pingping Song, Jing Chen, Yuanzhi Xu, Shengcai Qi, Ji Ma, Qiuhui Pan
PURPOSE: Neurotrophin receptor-interacting MAGE homologue (Nrage) plays an important role in bone development and the metabolism of normal skeletal structures. Our previous study showed that Nrage inhibited the odontogenic differentiation of mouse dental pulp cells. However, the potential roles and mechanism of Nrage in regulating odontogenic differentiation are unknown. The aim of this study was to investigate the molecular mechanism of Nrage in odontogenic differentiation of mouse odontoblast-like cells...
February 15, 2018: Connective Tissue Research
Brianne K Connizzo, Alan J Grodzinsky
Tendinopathy is a significant clinical problem thought to be associated with altered mechanical loading. Explant culture models allow researchers to alter mechanical loading in a controlled in vitro environment while maintaining tenocytes in their native matrix. However, current models do not accurately represent commonly injured tendons, ignoring contributions of associated musculature and bone, as well as regional collagen structure. This study details the characterization of a mouse rotator cuff explant culture model, including bone, tendon, and muscle (BTM)...
February 15, 2018: Connective Tissue Research
Chen Xiaodan, Song Zijun, Chen Rui, Tan Shuyi, Huang Chunhuang, Liu Yanhui, Cheng Bin, Fu Qiang
Lysophosphatidic acid is a serum-derived growth factor that is involved in wound healing. Although in its infancy, a growing body of evidence has demonstrated that lysophosphatidic acid exerts a potentially significant role in regulating bone cell biology. However, previous studies mainly focused on the osteoinductive potential of lysophosphatidic acid, its effects on bone tissue vascularization, another essential element during bone regeneration, remains ill-defined so far. Here in this study, we examined the effects of lysophosphatidic acid on osteogenic differentiation as well as the angiogenesis-inducing capacity of pre-osteoblasts, a cell population that coordinates osteogenic and angiogenic processes in bone regenerating niche...
February 15, 2018: Connective Tissue Research
Katie J Sikes, Jun Li, Shu-Guang Gao, Quan Shen, John D Sandy, Anna Plaas, Vincent M Wang
Healthy tendons are maintained in homeostasis through controlled usage of glucose for energy and redox equilibrium. Tendon cell stress imposed by overuse injury or vascular insufficiency is accompanied by activation of wound healing pathways which facilitate an adaptive response and the restoration of homeostasis. To understand this response at the gene expression level we have studied the in vivo effects of injected TGF-β1 in a murine model of tendinopathy, as well as treatment of murine tendon explants with either TGF-β1 or hypoxia in vitro...
February 15, 2018: Connective Tissue Research
Chunli Wang, Chunming Xu, Rongfu Chen, Li Yang, Kl Paul Sung
Purposes The anterior cruciate ligament (ACL) has poor functional healing response. The synovial tissue surrounding ACL ligament might be a major regulator of the microenvironment in the joint cavity after ACL injury, thus affecting the repair process. Using transwell co-culture, this study explored the direct influence of human synovial cells (HSCs) on ACL fibroblasts (ACLfs) by characterizing the differential expression of the lysyl oxidase family (LOXs) and matrix metalloproteinases (MMP-1, -2, -3), which facilitate extracellular matrix (ECM) repair and degradation, respectively...
February 12, 2018: Connective Tissue Research
Pâmella Coelho Dias, Pedro Henrique Justino Oliveira Limirio, Camila Rodrigues Borges Linhares, Mariana Lobo Bergamini, Flaviana Soares Rocha, Richarlisson Borges de Morais, Ana Paula Coelho Balbi, Karen Renata Nakamura Hiraki, Paula Dechichi
PURPOSE: The aim of this study was evaluate the effect of HBO on diabetic rats. MATERIALS AND METHODS: Twenty rats were distributed into four groups (n = 5): Control (C); Control + HBO (CH); Diabetes (D) and Diabetes + HBO (DH). Diabetes was induced by streptozotocin, and bone defects were created in both femurs in all animals. HBO therapy began immediately after surgery and was performed daily in the CH and DH groups. After 7 days, the animals were euthanized...
January 29, 2018: Connective Tissue Research
Agata Cieślik-Bielecka, Marcin Pierchała, Aleksandra Królikowska, Paweł Reichert
AIM OF THE STUDY: The aim of the study was to evaluate the effect of leukocyte- and platelet-rich plasma (L-PRP) treatment on a noncomplicated would healing after surgical skin incision in an experimental animal model. MATERIALS AND METHODS: The blood from 64 male Wistar rats was used to prepare L-PRP before a transverse dorsal incision was made and consecutively closed. At the same time after the surgery was completed, autologous L-PRP in the rats from the L-PRP group (n = 32) and NaCl in the control group (n = 32) were injected subcutaneously into the wound...
January 9, 2018: Connective Tissue Research
Letizia Rinella, Francesca Marano, Laura Paletto, Marco Fraccalvieri, Laura Annaratone, Isabella Castellano, Nicoletta Fortunati, Alessandro Bargoni, Laura Berta, Roberto Frairia, Maria Graziella Catalano
PURPOSES: Incomplete tendon healing impairs the outcome of tendon ruptures and tendinopathies. Human Adipose-derived Stem Cells (hASCs) are promising for tissue engineering applications. Extracorporeal Shock Waves (ESW) are a leading choice for the treatment of several tendinopathies. In this study, we investigated the effects of ESW treatment and tenogenic medium on the differentiation of hASCs into tenoblast-like cells. MATERIALS AND METHODS: hASCs were treated with ESW generated by a piezoelectric device and tenogenic medium...
January 9, 2018: Connective Tissue Research
Yanmin Bu, Dezhi Zheng, Lei Wang, Jun Liu
OBJECTIVE: This study aims to investigate the role of TSIX/miR-30a-5p axis in particle-induced osteolysis (PIO). METHOD: PIO mouse model was established by the implantation of Co-Cr-Mo metal particles (CoPs). MC3T3-E1 cells received CoPs stimulation. Bone mineral density (BMD) in the skull was detected to evaluate PIO development. The expression of TSIX and miR-30a-5p was detected by using qRT-PCR. Osteoblast apoptosis was measured using flow cytometry. RNA pull-down was used to verify the regulatory relationship between TSIX and miR-30a-5p...
December 20, 2017: Connective Tissue Research
Lauren B Radakovich, Angela J Marolf, John P Shannon, Stephen C Pannone, Vanessa D Sherk, Kelly S Santangelo
AIM: There is potential discrepancy between human and laboratory animal studies of osteoarthritis (OA), as radiographic assessment is the hallmark of the former and histopathology the standard for the latter. This suggests a need to evaluate OA in animal models in a manner similar to that utilized in people. Our study aimed to develop a whole joint grading scheme for microcomputed tomography (microCT) images in Hartley guinea pigs, a strain that recapitulates joint changes highlighted in human spontaneous OA...
December 11, 2017: Connective Tissue Research
Srujana Vedicherla, Sara Romanazzo, Daniel J Kelly, Conor T Buckley, Cathal J Moran
Purpose/aim of study: Menisectomies account for over 1.5 million surgical interventions in Europe annually, and there is a growing interest in regenerative strategies to improve outcomes in meniscal replacement. The overall objective of this study was to evaluate the role of intraoperatively applied fresh chondrocyte (FC) isolates compared to minced cartilage (MC) fragments, used without cell isolation, to improve bioactivity and tissue integration when combined with a polyurethane replacement. MATERIALS AND METHODS: First, to optimize the intraoperative cell isolation protocol, caprine articular cartilage biopsies were digested with 750 U/ml or 3000 U/ml collagenase type II (ratio of 10 ml per g of tissue) for 30 min, 1 h or 12 h with constant agitation and compared to culture-expanded chondrocytes in terms of matrix deposition when cultured on polyurethane scaffolds...
November 28, 2017: Connective Tissue Research
Anne Q Phan, Maurizio Pacifici, Jeffrey D Esko
Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth plates of skeletal elements in young patients. These benign tumors can affect growth plate function, leading to skeletal growth retardation, or deformations, and can encroach on nerves, tendons, muscles, and other surrounding tissues and cause motion impairment, chronic pain, and early onset osteoarthritis...
November 3, 2017: Connective Tissue Research
Hiroshi Watanabe, Hirotaka Ishii, Kenji Takahashi, Shinro Takai, Hitoshi Ozawa
Osteoarthritis (OA) is the leading cause of musculoskeletal disability in the elderly. Insights into the biological features of OA are obtained by characterization of the molecular features by gene expression profiling using reverse transcription-quantitative PCR (RT-qPCR). However, it has recently become evident that the use of suitable reference genes is required for appropriate normalization of this technique. Here total RNA was isolated from the synovium of 18 men and 20 women who underwent total knee arthroplasty for knee OA (KOA)...
November 2, 2017: Connective Tissue Research
Shozaburo Terai, Yusuke Hashimoto, Kumi Orita, Shinya Yamasaki, Junsei Takigami, Takafumi Shinkuma, Takanori Teraoka, Yohei Nishida, Masafumi Takahashi, Hiroaki Nakamura
We previously reported that circulating peripheral blood-borne cells (PBCs) contribute to early-phase meniscal reparative change. Because macrophages and myofibroblasts are important contributors of tissue regeneration, we examined their origin and distribution in the reparative meniscus. Reparative menisci were evaluated at 1, 2, and 4 weeks post-meniscectomy by immunohistochemistry to locate monocytes and macrophages (stained positive for CD68 and CD163), and myofibroblasts (stained positive for αSMA). Of the total number of cells, 13% were CD68(+) at 1 week post-meniscectomy, which decreased to 1% by 4 weeks post-meniscectomy; of these, almost half of CD68(+) cells (49...
November 2017: Connective Tissue Research
Cheul Hong Kim, Seong Soon Jeong, Ji Young Yoon, Ji Uk Yoon, Su Bin Yu, Eun Jung Kim
PURPOSE: Excess reactive oxygen species are detrimental to wound repair. Remifentanil decreases reactive oxygen species generation and inflammatory response; however, its effects on oxidative cell injury are not completely understood. Therefore, we investigated the effects of remifentanil on human keratinocytes under hydrogen peroxide-induced oxidative stress and the correlation of these effects with autophagy. MATERIALS AND METHODS: Human keratinocytes (HaCaT cell line) were randomly assigned to four groups: control, hydrogen peroxide, remifentanil pretreatment + hydrogen peroxide, and 3-methyladenine + remifentanil pretreatment + hydrogen peroxide...
November 2017: Connective Tissue Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"