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Connective Tissue Research

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https://www.readbyqxmd.com/read/29316815/effect-of-l-prp-treatment-on-wound-healing-after-surgical-skin-incision-in-an-experimental-animal-model
#1
Agata Cieślik-Bielecka, Marcin Pierchała, Aleksandra Królikowska, Paweł Reichert
AIM OF THE STUDY: The aim of the study was to evaluate the effect of leukocyte- and platelet-rich plasma (L-PRP) treatment on a noncomplicated would healing after surgical skin incision in an experimental animal model. MATERIALS AND METHODS: The blood from 64 male Wistar rats was used to prepare L-PRP before a transverse dorsal incision was made and consecutively closed. At the same time after the surgery was completed, autologous L-PRP in the rats from the L-PRP group (n = 32) and NaCl in the control group (n = 32) were injected subcutaneously into the wound...
January 9, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29316809/extracorporeal-shock-waves-trigger-tenogenic-differentiation-of-human-adipose-derived-stem-cells
#2
Letizia Rinella, Francesca Marano, Laura Paletto, Marco Fraccalvieri, Laura Annaratone, Isabella Castellano, Nicoletta Fortunati, Alessandro Bargoni, Laura Berta, Roberto Frairia, Maria Graziella Catalano
PURPOSES: Incomplete tendon healing impairs the outcome of tendon ruptures and tendinopathies. Human Adipose-derived Stem Cells (hASCs) are promising for tissue engineering applications. Extracorporeal Shock Waves (ESW) are a leading choice for the treatment of several tendinopathies. In this study, we investigated the effects of ESW treatment and tenogenic medium on the differentiation of hASCs into tenoblast-like cells. MATERIALS AND METHODS: hASCs were treated with ESW generated by a piezoelectric device and tenogenic medium...
January 9, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29260905/lncrna-tsix-promotes-osteoblast-apoptosis-in-particle-induced-osteolysis-by-down-regulating-mir-30a-5p
#3
Yanmin Bu, Dezhi Zheng, Lei Wang, Jun Liu
OBJECTIVE: This study aims to investigate the role of TSIX/miR-30a-5p axis in particle-induced osteolysis (PIO). METHOD: PIO mouse model was established by the implantation of Co-Cr-Mo metal particles (CoPs). MC3T3-E1 cells received CoPs stimulation. Bone mineral density (BMD) in the skull was detected to evaluate PIO development. The expression of TSIX and miR-30a-5p was detected by using qRT-PCR. Osteoblast apoptosis was measured using flow cytometry. RNA pull-down was used to verify the regulatory relationship between TSIX and miR-30a-5p...
December 20, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29226725/development-of-a-microcomputed-tomography-scoring-system-to-characterize-disease-progression-in-the-hartley-guinea-pig-model-of-spontaneous-osteoarthritis
#4
Lauren B Radakovich, Angela J Marolf, John P Shannon, Stephen C Pannone, Vanessa D Sherk, Kelly S Santangelo
AIM: There is potential discrepancy between human and laboratory animal studies of osteoarthritis (OA), as radiographic assessment is the hallmark of the former and histopathology the standard for the latter. This suggests a need to evaluate OA in animal models in a manner similar to that utilized in people. Our study aimed to develop a whole joint grading scheme for microcomputed tomography (microCT) images in Hartley guinea pigs, a strain that recapitulates joint changes highlighted in human spontaneous OA...
December 11, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29182439/chondrocyte-based-intraoperative-processing-strategies-for-the-biological-augmentation-of-a-polyurethane-meniscus-replacement
#5
Srujana Vedicherla, Sara Romanazzo, Daniel J Kelly, Conor T Buckley, Cathal J Moran
Purpose/aim of study: Menisectomies account for over 1.5 million surgical interventions in Europe annually, and there is a growing interest in regenerative strategies to improve outcomes in meniscal replacement. The overall objective of this study was to evaluate the role of intraoperatively applied fresh chondrocyte (FC) isolates compared to minced cartilage (MC) fragments, used without cell isolation, to improve bioactivity and tissue integration when combined with a polyurethane replacement. MATERIALS AND METHODS: First, to optimize the intraoperative cell isolation protocol, caprine articular cartilage biopsies were digested with 750 U/ml or 3000 U/ml collagenase type II (ratio of 10 ml per g of tissue) for 30 min, 1 h or 12 h with constant agitation and compared to culture-expanded chondrocytes in terms of matrix deposition when cultured on polyurethane scaffolds...
November 28, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29099240/advances-in-the-pathogenesis-and-possible-treatments-for-multiple-hereditary-exostoses-from-the-2016-international-mhe-conference
#6
Anne Q Phan, Maurizio Pacifici, Jeffrey D Esko
Multiple hereditary exostoses (MHE) is an autosomal dominant disorder that affects about 1 in 50,000 children worldwide. MHE, also known as hereditary multiple exostoses (HME) or multiple osteochondromas (MO), is characterized by cartilage-capped outgrowths called osteochondromas that develop adjacent to the growth plates of skeletal elements in young patients. These benign tumors can affect growth plate function, leading to skeletal growth retardation, or deformations, and can encroach on nerves, tendons, muscles, and other surrounding tissues and cause motion impairment, chronic pain, and early onset osteoarthritis...
November 3, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29095075/suitable-reference-gene-selection-for-gene-expression-studies-in-knee-osteoarthritis-synovium-using-quantitative-pcr-analysis
#7
Hiroshi Watanabe, Hirotaka Ishii, Kenji Takahashi, Shinro Takai, Hitoshi Ozawa
Osteoarthritis (OA) is the leading cause of musculoskeletal disability in the elderly. Insights into the biological features of OA are obtained by characterization of the molecular features by gene expression profiling using reverse transcription-quantitative PCR (RT-qPCR). However, it has recently become evident that the use of suitable reference genes is required for appropriate normalization of this technique. Here total RNA was isolated from the synovium of 18 men and 20 women who underwent total knee arthroplasty for knee OA (KOA)...
November 2, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29083939/growth-of-second-stage-mineral-in-lytechinus-variegatus
#8
S R Stock, Jong Seto, A C Deymier, A Rack, A Veis
Purpose and Aims: Sea urchin teeth consist of calcite and form in two stages with different magnesium contents. The first stage structures of independently formed plates and needle-prisms define the shape of the tooth, and the columns of the second stage mineral cements the first stage structures together and control the fracture behavior of the mature tooth. This study investigates the nucleation and growth of the second stage mineral. MATERIALS AND METHODS: Scanning electron microscopy (SEM) and synchrotron microComputed Tomography characterized the structures of the second phase material found in developing of Lytechinus variegatus teeth...
October 30, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28165810/the-origin-and-distribution-of-cd68-cd163-and-%C3%AE-sma-cells-in-the-early-phase-after-meniscal-resection-in-a-parabiotic-rat-model
#9
Shozaburo Terai, Yusuke Hashimoto, Kumi Orita, Shinya Yamasaki, Junsei Takigami, Takafumi Shinkuma, Takanori Teraoka, Yohei Nishida, Masafumi Takahashi, Hiroaki Nakamura
We previously reported that circulating peripheral blood-borne cells (PBCs) contribute to early-phase meniscal reparative change. Because macrophages and myofibroblasts are important contributors of tissue regeneration, we examined their origin and distribution in the reparative meniscus. Reparative menisci were evaluated at 1, 2, and 4 weeks post-meniscectomy by immunohistochemistry to locate monocytes and macrophages (stained positive for CD68 and CD163), and myofibroblasts (stained positive for αSMA). Of the total number of cells, 13% were CD68(+) at 1 week post-meniscectomy, which decreased to 1% by 4 weeks post-meniscectomy; of these, almost half of CD68(+) cells (49...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28165802/remifentanil-reduced-the-effects-of-hydrogen-peroxide-induced-oxidative-stress-in-human-keratinocytes-via-autophagy
#10
Cheul Hong Kim, Seong Soon Jeong, Ji Young Yoon, Ji Uk Yoon, Su Bin Yu, Eun Jung Kim
PURPOSE: Excess reactive oxygen species are detrimental to wound repair. Remifentanil decreases reactive oxygen species generation and inflammatory response; however, its effects on oxidative cell injury are not completely understood. Therefore, we investigated the effects of remifentanil on human keratinocytes under hydrogen peroxide-induced oxidative stress and the correlation of these effects with autophagy. MATERIALS AND METHODS: Human keratinocytes (HaCaT cell line) were randomly assigned to four groups: control, hydrogen peroxide, remifentanil pretreatment + hydrogen peroxide, and 3-methyladenine + remifentanil pretreatment + hydrogen peroxide...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28121187/development-and-progression-of-immobilization-induced-skin-fibrosis-through-overexpression-of-transforming-growth-factor-%C3%A3-1-and-hypoxic-conditions-in-a-rat-knee-joint-contracture-model
#11
Kyo Goto, Junya Sakamoto, Jiro Nakano, Hideki Kataoka, Yuichiro Honda, Ryo Sasabe, Tomoki Origuchi, Minoru Okita
PURPOSE: The purpose of this study was to investigate the pathology and mechanism of immobilization-induced skin fibrosis in a rat joint contracture model. METHODS: Rats were randomly divided into control and immobilization groups. In the immobilization groups, knee joints of the rats were immobilized for 1, 2, and 4 weeks. After each immobilization, skin was dissected. To assess fibrosis in the skin, the thickness and area of adipocytes and connective tissue fibers were measured...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28102712/bone-morphogenetic-protein-2-and-7-mediate-the-anabolic-function-of-nucleus-pulposus-cells-with-discrete-mechanisms
#12
Victor Y L Leung, Lixiong Zhou, Wai-Kit Tam, Yi Sun, Fengjuan Lv, Guangqian Zhou, Kenneth M C Cheung
Bone morphogenetic proteins (BMPs) play roles in promoting cell anabolism, especially in extracellular matrix production. The difference between BMP members in their capacity to modulate intervertebral disc cell activity is yet to be defined. BMP-7/OP-1 has been shown to retard disc degeneration. We compared the activity of BMP-7 with that of BMP-2 on nucleus pulposus (NP) cell phenotype and function, and investigated how they differentially affect the gene expression profiles of signaling cascade components in human NP cells under degenerative states...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28095112/autogenous-bone-particle-titanium-fiber-composites-for-bone-regeneration-in-a-rabbit-radius-critical-size-defect-model
#13
Huanxin Xie, Ye Ji, Qi Tian, Xintao Wang, Nan Zhang, Yicai Zhang, Jun Xu, Nanxiang Wang, Jinglong Yan
PURPOSE: To explore the effects of autogenous bone particle/titanium fiber composites on repairing segmental bone defects in rabbits. MATERIALS AND METHODS: A model of bilateral radial bone defect was established in 36 New Zealand white rabbits which were randomly divided into 3 groups according to filling materials used for bilaterally defect treatment: in group C, 9 animal bone defect areas were prepared into simple bilateral radius bone defect (empty sham) as the control group; 27 rabbits were used in groups ABP and ABP-Ti...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27937051/wnt10b-and-dkk-1-gene-therapy-differentially-influenced-trabecular-bone-architecture-soft-tissue-integrity-and-osteophytosis-in-a-skeletally-mature-rat-model-of-osteoarthritis
#14
Jeffrey B Mason, Brittney L Gurda, Kurt D Hankenson, Lindsey R Harper, Cathy S Carlson, James M Wilson, Dean W Richardson
AIMS: Our goals in the current experiments were to determine if (a) upregulation of Wnt signaling would induce osteoarthritis changes in stable stifle joints and (b) if downregulation of Wnt signaling in destabilized joints would influence the progression of OA. METHODS: At 37 weeks of age, rats were injected in the stifle joint with a recombinant adeno-associated viral vector containing the Wnt-inhibitor Dkk-1 or a Wnt10b transgene. At 40 weeks of age, rats underwent surgical destabilization of the joint...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27929701/effect-of-serum-and-platelet-rich-plasma-on-human-early-or-advanced-degenerative-meniscus-cells
#15
Undine Freymann, Lucia Degrassi, Jan Philipp Krüger, Sebastian Metzlaff, Michaela Endres, Wolf Petersen
PURPOSE: The purpose of this in vitro study was to evaluate the migratory, proliferating, and extracellular matrix (ECM) forming effect of human serum (HS) and platelet-rich plasma (PRP) on meniscus cells derived from human knees with early or advanced degenerative changes. MATERIALS AND METHODS: Medial menisci from knees with early degenerative changes (n = 5; mean Kellgren score of 1) undergoing arthroscopic meniscal surgery and advanced degenerative changes (n = 5; mean Kellgren score of 4) undergoing total knee replacement were collected...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27905856/1-25-dihydroxyvitamin-d3-stimulates-odontoblastic-differentiation-of-human-dental-pulp-stem-cells-in-vitro
#16
Goksen Mucuk, Elif Sepet, Mine Erguven, Ozlem Ekmekcı, Ayhan Bılır
BACKGROUND: 1,25-Dihydroxyvitamin D3 (1,25-OH D3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). AIM: This study aimed to understand the influence of various concentrations of 1,25-OH D3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. MATERIALS AND METHODS: hDPSCs were obtained from the impacted third molar teeth...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27898233/hyaluronan-stimulates-chondrogenic-gene-expression-in-human-meniscus-cells
#17
Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshifumi Ozaki
Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. MATERIALS AND METHODS: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco's modified Eagle's medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2-7 days...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27668694/autophagy-in-osteoarthritis
#18
Hyelin Jeon, Gun-Il Im
Osteoarthritis is characterized by continuous degeneration of articular cartilage resulting in disability. The death of chondrocytes and the loss of the extracellular matrix are the central peculiarities in cartilage degeneration during osteoarthritis pathogenesis. Autophagy is an essential cellular homeostasis mechanism whereby cellular organelles and macromolecules are recycled to maintain cellular metabolism. Autophagy is reported to be cytoprotective effects for articular cartilage, and osteoarthritis is associated with decreased autophagy...
November 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/29035127/healing-potential-of-nanohydroxyapatite-gelatin-and-fibrin-platelet-glue-combination-as-tissue-engineered-scaffolds-in-radial-bone-defects-of-rats
#19
Abdolhamid Meimandi-Parizi, Ahmad Oryan, Hojjat Gholipour
Different biomaterials have been used in orthopedic surgery. Evaluation of biomaterials for bone healing promotion has been a wide area of research of the orthopedic field. Sixty critical size defects of 5 mm long were bilaterally created in the radial diaphysis of 30 rats. The animals were randomly divided into six equal groups as empty defect, autograft, nanohydroxyapatite (nHA), Gelatin (Gel)-nHA, fibrin-platelet glue (FPG)-nHA, and Gel-FPG-nHA groups (n = 10 in each group). Radiographs of each forelimb were taken postoperatively on the 1st day and then at the 28th and 56th days post injury...
October 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28956662/leptin-alone-and-in-combination-with-interleukin-1-beta-induced-cartilage-degradation-potentially-inhibited-by-epa-and-dha
#20
Thanyaluck Phitak, Kanchanit Boonmaleerat, Peraphan Pothacharoen, Dumnoensun Pruksakorn, Prachya Kongtawelert
Osteoarthritis (OA) is the most common form of arthritis. Obesity has been believed to be an important risk factor for OA development and the progression of not only load-bearing joints, but low-load-bearing joints as well. Increased leptin has been the focus of a link between obesity and OA. In this study, the effects of pathological (100ng/ml) or supra-pathological (10μg/ml) concentrations of leptin alone or in combination with IL1β on cartilage metabolisms were studied in porcine cartilage explant. The involved mechanisms were examined in human articular chondrocytes (HACs)...
September 28, 2017: Connective Tissue Research
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