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Connective Tissue Research

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https://www.readbyqxmd.com/read/28102713/factors-associated-with-the-status-of-meniscal-tears-following-meniscal-repair-concomitant-with-anterior-cruciate-ligament-reconstruction
#1
Takehiko Matsushita, Kanto Nagai, Daisuke Araki, Toshikazu Tanaka, Tomoyuki Matsumoto, Kotaro Nishida, Masahiro Kurosaka, Ryosuke Kuroda
Purpose Anterior cruciate ligament (ACL) injuries are often accompanied by meniscal tears. Meniscal repair is typically performed during ACL reconstruction. However, retear of the meniscus after repair is frequent. The purpose of this study was to analyze the factors associated with meniscal healing after meniscal repair concomitant with ACL reconstruction. Materials and Methods Eighty-seven meniscal repairs in 87 patients who received meniscal repair combined with ACL reconstruction and received second-look arthroscopy at a mean of postoperatively 15...
January 19, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28102712/bone-morphogenetic-protein-2-and-7-mediate-the-anabolic-function-of-nucleus-pulposus-cells-with-discrete-mechanisms
#2
Victor Yl Leung, Lixiong Zhou, Wai-Kit Tam, Yi Sun, Fengjuan Lv, Guangqian Zhou, Kenneth Mc Cheung
Bone morphogenetic proteins (BMPs) play roles in promoting cell anabolism, especially extracellular matrix production. The difference between BMP members in their capacity to modulate intervertebral disc cell activity is yet to be defined. BMP-7/OP-1 has been shown to retard disc degeneration. We compared the activity of BMP-7 with BMP-2 on nucleus pulposus (NP) cell phenotype and function, and investigated how they differentially affect the gene expression profiles of signaling cascade components in human NP cells under degenerative states...
January 19, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28095112/autogenous-bone-particle-titanium-fibre-composites-for-bone-regeneration-in-a-rabbit-radius-critical-size-defect-model
#3
Huanxin Xie, Ye Ji, Qi Tian, Xintao Wang, Nan Zhang, Yicai Zhang, Jun Xu, Nanxiang Wang, Jinglong Yan
PURPOSE: To explore the effects of autogenous bone particle/titanium fibre composites on repairing segmental bone defects in rabbits. MATERIALS AND METHODS: A model of bilateral radial bone defect was established in 36 New Zealand white rabbits which were randomly divided into 3 groups according to filling materials used for bilaterally defect treatment: in group C, 9 animal bone defect areas were prepared into simple bilateral radius bone defect (empty sham) as the control group; 27 rabbits were used in groups ABP and ABP-Ti...
January 17, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28095064/adipokines-induce-catabolism-of-newly-synthesized-matrix-in-cartilage-and-meniscus-tissues
#4
James F Nishimuta, Marc E Levenston
PURPOSE: Altered synovial levels of various adipokines (factors secreted by fat as well as other tissues) have been associated with osteoarthritis onset and progression. However, the metabolic effects of adipokines on joint tissues, in particular the fibrocartilaginous menisci, are not well understood. This study investigated effects of several adipokines on release of recently synthesized extracellular matrix in bovine cartilage and meniscus tissue explants. MATERIALS AND METHODS: After labeling newly synthesized proteins and sulfated glycosaminoglycans (sGAGs) with (3)H-proline and (35)S-sulfate, respectively, bovine cartilage and meniscus tissue explants were cultured for 6 days in basal medium (control) or media supplemented with adipokines (1 µg/ml of leptin, visfatin, adiponectin, or resistin) or 20ng/ml interleukin-1 (IL-1)...
January 17, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28051883/hydrogels-for-precision-meniscus-tissue-engineering-a-comprehensive-review
#5
Ana Rey-Rico, Magali Cucchiarini, Henning Madry
The meniscus plays a pivotal role to preserve the knee joint homeostasis. Lesions to the meniscus are frequent, have a reduced ability to heal, and may induce tibiofemoral osteoarthritis. Current reconstructive therapeutic options mainly focus on the treatment of lesions in the peripheral vascularized region. In contrast, few approaches are capable of stimulating repair of damaged meniscal tissue in the central, avascular portion. Tissue engineering approaches are of high interest to repair or replace damaged meniscus tissue in this area...
January 4, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28026970/engineering-3d-bioplotted-scaffolds-to-induce-aligned-extracellular-matrix-deposition-for-musculoskeletal-soft-tissue-replacement
#6
Paul B Warren, Pedro Huebner, Jeffrey T Spang, Rohan A Shirwaiker, Matthew B Fisher
PURPOSE: Tissue engineering and regenerative medicine approaches have the potential to overcome the challenges associated with current treatment strategies for meniscus injuries. 3D-Bioplotted scaffolds are promising, but have not demonstrated the ability to guide the formation of aligned collagenous matrix in vivo, which is critical for generating functional meniscus tissue. In this study, we evaluate the ability of 3D-Bioplotted scaffold designs with varying interstrand spacing to induce the deposition of aligned matrix in vivo...
December 27, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/28005443/identification-and-characterization-of-adult-mouse-meniscus-stem-progenitor-cells
#7
Laura W Gamer, Rui Rui Shi, Ashira Gendelman, Dylan Mathewson, Jackson Gamer, Vicki Rosen
Meniscal damage is a common problem that accelerates the onset of knee osteoarthritis. Stem cell based tissue engineering treatment approaches have shown promise in preserving meniscal tissue and restoring meniscal function. The purpose of our study was to identify meniscus-derived stem/progenitor cells (MSPCs) from mouse, a model system that allows for in vivo analysis of the mechanisms underlying meniscal injury and healing. MSPCs were isolated from murine menisci grown in explant culture and characterized for stem cell properties...
December 22, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27937051/wnt10b-and-dkk-1-gene-therapy-differentially-influenced-trabecular-bone-architecture-soft-tissue-integrity-and-osteophytosis-in-a-skeletally-mature-rat-model-of-osteoarthritis
#8
Jeffrey B Mason, Brittney L Gurda, Kurt D Hankenson, Lindsey R Harper, Cathy S Carlson, James M Wilson, Dean W Richardson
AIMS: Our goals in the current experiments were to determine if 1) up-regulation of Wnt signaling would induce OA changes in stable stifle joints and 2) if down-regulation of Wnt signaling in destabilized joints would influence the progression of OA. METHODS: At 37 weeks of age, rats were injected in the stifle joint with a recombinant adeno-associated viral vector containing the Wnt-inhibitor Dkk-1 or a Wnt10b transgene. At 40 weeks of age, rats underwent surgical destabilization of the joint...
December 9, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27929701/effect-of-serum-and-platelet-rich-plasma-on-human-early-or-advanced-degenerative-meniscus-cells
#9
Undine Freymann, Lucia Degrassi, Jan Philipp Krüger, Sebastian Metzlaff, Michaela Endres, Wolf Petersen
PURPOSE: The purpose of this in vitro study was to evaluate the migratory, proliferating & extracellular matrix (ECM) forming effect of human serum (HS) and platelet-rich plasma (PRP) on meniscus cells derived from human knees with early or advanced degenerative changes. MATERIALS AND METHODS: Medial menisci from knees with early degenerative changes (n=5; mean Kellgren score of 1) undergoing arthroscopic meniscal surgery and advanced degenerative changes (n=5; mean Kellgren score of 4) undergoing total knee replacement were collected...
December 8, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27925477/relevance-of-meniscal-cell-regional-phenotype-to-tissue-engineering
#10
Shawn P Grogan, Chantal Pauli, Martin K Lotz, Darryl D D'Lima
PURPOSE: Meniscus contains heterogeneous populations of cells that have not been fully characterized. Cell phenotype is often lost during culture; however, culture expansion is typically required for tissue engineering. We examined and compared cell surface molecule expression levels on human meniscus cells from the vascular and avascular regions and articular chondrocytes while documenting changes during culture-induced dedifferentiation. MATERIALS AND METHODS: Expression of 16 different surface molecules were examined by flow cytometry after monolayer culture for 24 h, 1 week, and 2 weeks...
December 7, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27925474/fiber-development-and-matrix-production-in-tissue-engineered-menisci-using-bovine-mesenchymal-stem-cells-and-fibrochondrocytes
#11
Mary Clare McCorry, Lawrence J Bonassar
Mesenchymal stem cells (MSCs) have been investigated with promising results for meniscus healing and tissue engineering. While MSCs are known to contribute to ECM production, less is known about how MSCs produce and align large organized fibers for application to tissue engineering the meniscus. The goal of this study was to investigate the capability of MSCs to produce and organize extracellular matrix molecules compared to meniscal fibrochondrocytes (FCCs). Bovine FCCs and MSCs were encapsulated in an anatomically accurate collagen meniscus using mono-culture and co-culture of each cell type...
December 7, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27905856/1-25-dihydroxyvitamin-d3-stimulates-odontoblastic-differentiation-of-human-dental-pulp-stem-cells-in-vitro
#12
Goksen Mucuk, Elif Sepet, Mine Erguven, Ozlem Ekmekcı, Ayhan Bılır
BACKGROUND: 1,25-Dihydroxyvitamin D3 (1,25-OH D3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). AIM: This study aimed to understand the influence of various concentrations of 1,25-OH D3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. MATERIALS AND METHODS: hDPSCs were obtained from the impacted third molar teeth...
December 1, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27898233/hyaluronan-stimulates-chondrogenic-gene-expression-in-human-meniscus-cells
#13
Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshifumi Ozaki
: Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. MATERIALS AND METHODS: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco's modified Eagle's medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2-7 days...
November 29, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27892729/reduction-and-fragmentation-of-elastic-fibers-in-the-skin-of-obese-mice-is-associated-with-altered-mrna-expression-levels-of-fibrillin-1-and-neprilysin
#14
Hiroko Makihara, Moeko Hidaka, Yui Sakai, Yoshiko Horie, Hideaki Mitsui, Kenichi Ohashi, Yoshio Goshima, Tomoko Akase
AIM OF THE STUDY: Our previous research suggested that obesity induces structural fragility in the skin. Elastic fibers impart strength and elasticity. In this study, we determined whether elastic fibers decrease in the skin of obese mice. MATERIALS AND METHODS: To confirm alterations in elastic fiber content due to obesity, we used spontaneously obese model mice (TSOD) and control mice (TSNO). Furthermore, to evaluate the elastin structure and gene expression dependent on the severity of obesity, an obesity-enhanced mouse model was developed by feeding a high fat diet to TSOD (TSOD-HF)...
November 28, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27813681/cyclic-muscle-twitch-contraction-inhibits-immobilization-induced-muscle-contracture-and-fibrosis-in-rats
#15
Ayana Yoshimura, Junya Sakamoto, Yuichiro Honda, Hideki Kataoka, Jiro Nakano, Minoru Okita
We investigated the effects of cyclic muscle twitch contraction caused by neuromuscular electrical stimulation (NMES) on immobilization-induced muscle contracture and fibrosis in rats. Twenty-nine rats were divided into control, immobilization, and immobilization with muscle contraction groups. The ankle joints of the immobilization and muscle contraction rats were fixed in full plantar flexion with a plaster cast for 4 weeks. In the muscle contraction group, cyclic muscle twitch contraction of the soleus muscle was induced using a commercial device (1 Hz, 4 ± 2 mA, 60 min/day, 5 times/week) with the ankle joint immobilized...
November 4, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27813662/matrix-metalloproteinase-activity-and-prostaglandin-e2-are-elevated-in-the-synovial-fluid-of-meniscus-tear-patients
#16
Betty Liu, Adam P Goode, Teralyn E Carter, Gangadhar M Utturkar, Janet L Huebner, Dean C Taylor, Claude T Moorman Iii, William E Garrett, Virginia B Kraus, Farshid Guilak, Louis E DeFrate, Amy L McNulty
PURPOSE: Meniscus tears are a common knee injury and are associated with the development of post-traumatic osteoarthritis (OA). The purpose of this study is to evaluate potential OA mediators in the synovial fluid and serum of meniscus tear subjects compared to those in the synovial fluid of radiographic non-OA control knees. MATERIALS AND METHODS: Sixteen subjects with an isolated unilateral meniscus injury and six subjects who served as reference controls (knee Kellgren-Lawrence grade 0-1) were recruited...
November 4, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27797605/quantitative-histological-grading-methods-to-assess-subchondral-bone-and-synovium-changes-subsequent-to-medial-meniscus-transection-in-the-rat
#17
Heidi E Kloefkorn, Kyle D Allen
AIM OF THE STUDY: The importance of the medial meniscus to knee health is demonstrated by studies which show meniscus injuries significantly increase the likelihood of developing osteoarthritis (OA), and knee OA can be modeled in rodents using simulated meniscus injuries. Traditionally, histological assessments of OA in these models have focused on damage to the articular cartilage; however, OA is now viewed as a disease of the entire joint as an organ system. The aim of this study was to develop quantitative histological measures of bone and synovial changes in a rat medial meniscus injury model of knee OA...
October 31, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27791406/reconstruction-of-cranial-cruciate-ligament-in-rabbits-using-polyester-implants-saturated-with-prp-antlerogenic-stem-cells-mic-1-and-their-homogenate
#18
Przemysław Prządka, Zdzisław Kiełbowicz, Bogdan Osiński, Stanisław Dzimira, Janusz A Madej, Wojciech Nowacki, Krzysztof Kubiak, Paweł Reichert, Marek Cegielski
AIM OF THE STUDY: The attempt to limit the negative effects of polyester implants on the articular cavity by using preparations containing growth factors. MATERIALS AND METHODS: Polyester implants used for the reconstruction of a rabbit's cranial cruciate ligament (CCL) were saturated with autogenic platelet-rich plasma (PRP), antlerogenic stem cells MIC-1 and their homogenate prior to the surgery. Six months after CCL reconstruction, morphological, and biochemical blood tests were carried out, including proteinogram and acute phase proteins...
October 28, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27813659/recent-advances-in-epigenetic-regulation-of-skeletal-development-homeostasis-and-disease
#19
Audrey McAlinden
No abstract text is available yet for this article.
January 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/27763799/creating-conditional-dual-fluorescence-labeled-transgenic-animals-for-studying-function-of-small-noncoding-rnas
#20
Kun Yang, Yun Gao, Mingfu Yang, Zuoshang Xu, Qian Chen
Because the function of most noncoding (nc) RNAs is unknown, Cre-lox transgenic mice are useful tools to determine their functions in a tissue or developmental stage-specific manner. However, the technology faces challenges because expression of ncRNA-transgene lacks protein product. No antibody or peptide-tag can be used to trace ncRNA expression in mouse tissues in real time. Furthermore, transgene integration at different locus or orientations in the genome may result in recombination of genomic fragments in the Cre-lox system...
January 2017: Connective Tissue Research
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