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Connective Tissue Research

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https://www.readbyqxmd.com/read/29745261/streptozotocin-induced-diabetes-alters-transcription-of-multiple-genes-necessary-for-extracellular-matrix-remodeling-in-rat-patellar-tendon
#1
Shivam H Patel, Arman Sabbaghi, Chad C Carroll
OVERVIEW: Tendon collagen fibril degradation is commonly seen in tendons of diabetics, but the mechanisms responsible for these changes remain to be elucidated. We have demonstrated that streptozotocin (STZ)-induced diabetes increases tendon cell proliferation and collagen content. In the present study, we evaluated that impact of STZ-induced diabetes on mRNA transcripts involved with collagen fibril organization, extracellular matrix (ECM) remodeling, apoptosis, and proliferation. MATERIALS AND METHODS: Rats were divided into four groups: nondiabetic (control, n = 9), 1 week (acute, n = 8) or 10 weeks of diabetes (chronic, n = 7), and 10 weeks of diabetes with insulin (insulin, n = 8)...
May 10, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29732924/identification-and-characterization-of-a-novel-fbn1-gene-variant-in-an-extended-family-with-variable-clinical-phenotype-of-marfan-syndrome
#2
Mahmut Cerkez Ergoren, Burcu Turkgenc, Kerem Teralı, Orhan Rodopluu, Aline Verstraeten, Lut Van Laer, Gamze Mocan, Bart Loeys, Omer Tetik, Sehime G Temel
No abstract text is available yet for this article.
May 6, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29695173/conditioned-media-from-human-osteoarthritic-synovium-induces-inflammation-in-a-synoviocyte-cell-line
#3
Elisa Belluzzi, Eleonora Olivotto, Giovanna Toso, Augusto Cigolotti, Assunta Pozzuoli, Carlo Biz, Giovanni Trisolino, Pietro Ruggieri, Brunella Grigolo, Roberta Ramonda, Marta Favero
AIM: Osteoarthritis (OA) is a whole joint pathology involving cartilage, synovial membrane, meniscus, subchondral bone and infrapatellar fat pad (IFP). Synovitis has been widely documented in OA suggesting its important role in pathogenesis. The aim of this study was to investigate the role of different joint tissues in promoting synovitis. MATERIALS AND METHODS: Conditioned media (CM) from cartilage, synovial membrane, meniscus and IFP, were generated from tissues of five patients undergoing total knee replacement and used to stimulate a human fibroblast-like synoviocytes cell line (K4IM)...
April 26, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29658360/hyaluronic-acid-promotes-proliferation-and-migration-of-human-meniscus-cells-via-a-cd44-dependent-mechanism
#4
Tomohiko Murakami, Shuhei Otsuki, Yoshinori Okamoto, Kosuke Nakagawa, Hitoshi Wakama, Nobuhiro Okuno, Masashi Neo
PURPOSE: Treatment of meniscal injury is important for osteoarthritis (OA) prevention. Meniscus cells are divided between inner and outer cells, which have different characteristics and vascularity. We evaluated the effects of hyaluronic acid (HA) on the proliferation and migration of human inner and outer meniscus cells, and investigated the underlying healing mechanisms. MATERIALS AND METHODS: Lateral menisci from 18 patients who underwent total knee arthroplasty were used...
April 15, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29651864/exogenous-skeletal-muscle-satellite-cells-promote-the-repair-of-levator-palpebrae-superioris-mechanical-damage-in-rat
#5
Lin Ye, Yuanyuan Yao, Hui Guo, Yun Peng
AIM OF THE STUDY: Blepharoptosis is a drooping of the upper eyelid, usually due to dysfunction of the levator palpebrae superioris (LPS). Recently, skeletal muscle satellite cells (SSCs) have been reported to promote the repair of damaged skeletal muscle. This study aims to investigate the potential contribution of exogenous SSCs to the regeneration of mechanically damaged LPS. MATERIALS AND METHODS: Thirty-two rats were randomly divided into four groups, including control group, SSCs-treated group, SSCs-treated injury group and non-treated injury group...
April 13, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29649906/knockdown-of-lncrna-malat1-contributes-to-the-suppression-of-inflammatory-responses-by-up-regulating-mir-146a-in-lps-induced-acute-lung-injury
#6
Lingling Dai, Guojun Zhang, Zhe Cheng, Xi Wang, Liuqun Jia, Xiaogang Jing, Huan Wang, Rui Zhang, Meng Liu, Tianci Jiang, Yuanjian Yang, Meng Yang
BACKGROUND: Acute lung injury (ALI) is a type of severe pulmonary inflammatory disease with high rates of morbidity and mortality. Now, an increasing number of studies suggest that lncRNAs may act as key regulators of the inflammatory response and play a crucial role in the pathogenesis of many inflammatory diseases. Our study firstly explored the function and underlying mechanism of lncRNA metastasis-associated lung adenocarcinoma transcription 1 (MALAT1) in regulating the inflammatory response of lipopolysaccharide (LPS)-induced ALI in rats...
April 13, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29609502/fluid-shear-stress-promotes-osteoblast-proliferation-through-the-nfatc1-erk5-pathway
#7
Ning Ding, Bin Geng, Zhonghao Li, Quanzeng Yang, Liang Yan, Lang Wan, Bo Zhang, Cuifang Wang, Yayi Xia
PURPOSE: Extracellular signal regulated kinase 5 (ERK5) is thought to regulate osteoblast proliferation. To further understand how ERK5 signalling regulates osteoblast proliferation induced by fluid shear stress (FSS), we examined some potential signalling targets associated with ERK5 in MC3T3-E1 cells. METHODS: MC3T3-E1 cells were treated with XMD8-92 (an ERK5 inhibitor) or Cyclosporin A (CsA, a Nuclear Factor of Activated T Cells (NFAT) c1 inhibitor) and/or exposed to 12 dyn/cm2 FSS...
April 3, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29448860/therapeutic-ionizing-radiation-induced-bone-loss-a-review-of-in-vivo-and-in-vitro-findings
#8
Jian Zhang, Xinyu Qiu, Kedi Xi, Wentao Hu, Hailong Pei, Jing Nie, Ziyang Wang, Jiahan Ding, Peng Shang, Bingyan Li, Guangming Zhou
Radiation therapy is one of the routine treatment modalities for cancer patients. Ionizing radiation (IR) can induce bone loss, and consequently increases the risk of fractures with delayed and nonunion of the bone in the cancer patients who receive radiotherapy. The orchestrated bone remodeling can be disrupted due to the affected behaviors of bone cells, including bone mesenchymal stem cells (BMSCs), osteoblasts and osteoclasts. BMSCs and osteoblasts are relatively radioresistant compared with osteoclasts and its progenitors...
March 29, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29560747/critical-sized-cartilage-defects-in-the-equine-carpus
#9
Eve Salonius, Lassi Rieppo, Mikko J Nissi, Hertta J Pulkkinen, Harold Brommer, Anne Brünott, Tuomo S Silvast, P René Van Weeren, Virpi Muhonen, Pieter A J Brama, Ilkka Kiviranta
AIM: The horse joint, due to its similarity with the human joint, is the ultimate model for translational articular cartilage repair studies. This study was designed to determine the critical size of cartilage defects in the equine carpus and serve as a benchmark for the evaluation of new cartilage treatment options. MATERIAL AND METHODS: Circular full-thickness cartilage defects with a diameter of 2, 4, and 8 mm were created in the left middle carpal joint and similar osteochondral (3...
April 12, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29557203/effect-of-collagen-length-distribution-and-timing-for-repair-on-the-active-tgf-%C3%AE-concentration-in-tendon
#10
Arash Mehdizadeh, Bruce S Gardiner, Michael Lavagnino, David W Smith
The composition of extracellular matrix (ECM) in tendon depends on the secretion profile of resident cells known as tenocytes. For tissues with a mechanical role like tendon, mechanical strain is known to play an important role in determining the secretion profile of resident cells. Previously we explored the idea of estimating average concentrations of ECM molecules as a function of tendon strain magnitude and number of loading cycles. Specifically, we developed a model of the mechanical fatigue damage of tendon collagen fibers and introduced elementary cell responses (ECRs) by which local cellular-level responses to the strain environment, combined with the fatigue damage model, were scaled up to predict tissue-level responses...
March 20, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29447019/lysophosphatidic-acid-enhanced-the-osteogenic-and-angiogenic-capability-of-osteoblasts-via-lpa1-3-receptor
#11
Xiaodan Chen, Zijun Song, Rui Chen, Shuyi Tan, Chunhuang Huang, Yanhui Liu, Bin Cheng, Qiang Fu
Lysophosphatidic acid is a serum-derived growth factor that is involved in wound healing. Although in its infancy, a growing body of evidence has demonstrated that lysophosphatidic acid exerts a potentially significant role in regulating bone cell biology. However, previous studies mainly focused on the osteoinductive potential of lysophosphatidic acid, its effects on bone tissue vascularization, another essential element during bone regeneration, remains ill-defined so far. Here in this study, we examined the effects of lysophosphatidic acid on osteogenic differentiation as well as the angiogenesis-inducing capacity of pre-osteoblasts, a cell population that coordinates osteogenic and angiogenic processes in bone regenerating niche...
March 16, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29513041/vegf-mediated-angiogenesis-and-vascularization-of-a-fumarate-crosslinked-polycaprolactone-pclf-scaffold
#12
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J Van Wijnen, Michael J Yaszemski, Sanjeev Kakar
PURPOSE: Revascularization of natural and synthetic scaffolds is a critical part of the scaffold's incorporation and tissue ingrowth. Our goals were to create a biocompatible polymer scaffold with 3D-printing technology, capable of sustaining vascularization and tissue ingrowth. METHODS: We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow tissue ingrowth via large interconnected pores. The scaffolds were prepared with Poly(lactic-co-glycolic acid)(PLGA) microspheres seeded with or without different growth factors including VEGF,FGF-2, and/or BMP-2...
March 7, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29457525/interleukin-1%C3%AE-induces-apoptosis-in-annulus-fibrosus-cells-through-the-extracellular-signal-regulated-kinase-pathway
#13
Xing Yang, Lei Wang, Zhang-Qin Yuan, Ping-Hui Zhou, Geng-Lei Chu, Bin Li, Jun-Ying Sun
PURPOSE: The loss of intervertebral disc (IVD) cells due to excessive apoptosis induced by inflammatory cytokines is a major cause of IVD degeneration. This study aims to explore the mechanism of interleukin-1β (IL-1β)-induced apoptosis of annulus fibrosus cells (AFCs). It's hypothesized that IL-1β induces apoptosis through the extracellular signal-regulated kinase (ERK) pathway in AFCs. METHODS: The mRNA and protein expression levels of apoptosis-associated genes were analyzed by quantitative real-time PCR and Western blotting...
February 26, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745821/regulation-of-osteogenesis-by-long-noncoding-rnas-an-epigenetic-mechanism-contributing-to-bone-formation
#14
Coralee E Tye, Joseph R Boyd, Natalie A Page, Michelle M Falcone, Janet L Stein, Gary S Stein, Jane B Lian
Long noncoding RNAs (lncRNAs) have recently emerged as novel regulators of lineage commitment, differentiation, development, viability, and disease progression. Few studies have examined their role in osteogenesis; however, given their critical and wide-ranging roles in other tissues, lncRNAs are most likely vital regulators of osteogenesis. In this study, we extensively characterized lncRNA expression in mesenchymal cells during commitment and differentiation to the osteoblast lineage using a whole transcriptome sequencing approach (RNA-Seq)...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745820/late-stages-of-mineralization-and-their-signature-on-the-bone-mineral-density-distribution
#15
Pascal R Buenzli, Chloé Lerebours, Andreas Roschger, Paul Roschger, Richard Weinkamer
PURPOSE: Experimental measurements of bone mineral density distributions (BMDDs) enable a determination of secondary mineralization kinetics in bone, but the maximum degree of mineralization and how this maximum is approached remain uncertain. We thus test computationally different hypotheses on late stages of bone mineralization by simulating BMDDs in low-turnover conditions. MATERIALS AND METHODS: An established computational model of the BMDD that accounts for mineralization and remodeling processes was extended to limit mineralization to various maximum calcium capacities of bone...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745819/crosstalk-between-neuropeptides-sp-and-cgrp-in-regulation-of-bmp2-induced-bone-differentiation
#16
Ceren Tuzmen, Phil G Campbell
AIM OF THE STUDY: The peripheral nervous system is involved in regulation of bone metabolism via sensory and sympathetic innervation. Substance P (SP) and calcitonin gene-related peptide (CGRP) are two sensory neuropeptides that have been associated with regulation of osteogenic differentiation. However, the interaction between SP and CGRP both with each other and the bone morphogenetic protein 2 (BMP2) in regulation of osteogenic differentiation has not been studied. Therefore, the aim of this study was to investigate the interaction between SP and CGRP on BMP2-induced bone differentiation using model progenitor cells...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745818/novel-insights-into-renal-mineralization-and-stone-formation-through-advanced-imaging-modalities
#17
Scott V Wiener, Ling Chen, Alex R Shimotake, Misun Kang, Marshall L Stoller, Sunita P Ho
Purpose/Aim: The most common kidney stone composed of calcium oxalate forms on interstitial calcium phosphate mineral known as a Randall's plaque (RP). Due to limited information about events leading to the initial deposition of nanometer size interstitial calcium phosphate pre-clusters, there continues to be a debate on the initial site of calcium phosphate deposition and factors leading to stone formation. MATERIALS AND METHODS: High-resolution X-ray micro-computed tomography (CT), and light and electron microscopy techniques were used to characterize human renal pyramids and five representative kidney stones with identifiable stems...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745817/impaired-mineral-quality-in-dentin-in-x-linked-hypophosphatemia
#18
Benjamin R Coyac, Guillaume Falgayrac, Guillaume Penel, Alain Schmitt, Thorsten Schinke, Agnès Linglart, Marc D McKee, Catherine Chaussain, Claire Bardet
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745816/the-unique-biomineralization-transcriptome-and-proteome-of-lytechinus-variegatus-teeth
#19
Keith Alvares, Caroline J DeHart, Paul M Thomas, Neil L Kelleher, Arthur Veis
BACKGROUND: Matrix-regulated biomineralization involves the specific nucleation and growth of mineral phases within or upon preformed structured organic matrices. We hypothesized that there might be a general mechanism whereby anionic, phosphorylated mineral ion-binding proteins assist in specifically locating the mineral ions with respect to the mineralizing structural organic matrix. Here we extended these studies to invertebrate mineralization in Lytechinus variegatus (Lv) teeth. MATERIALS AND METHODS: The tooth proteins were extracted and the phosphoproteins occluded in the mineral were enriched by passage through a ProQ Diamond phosphoprotein enrichment column, and subjected to MS/MS analysis...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745815/physicochemical-analysis-of-human-pulpal-mineralization-secondary-to-fam20a-mutations
#20
Fleur Berès, Guilhem Lignon, Stéphan Rouzière, Cédric Mauprivez, Stéphane Simon, Ariane Berdal, Arnaud Dessombz
No abstract text is available yet for this article.
December 2018: Connective Tissue Research
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