journal
MENU ▼
Read by QxMD icon Read
search

Molecular and Cellular Biochemistry

journal
https://www.readbyqxmd.com/read/28083717/physiology-of-the-vc-nhap-paralogous-group-of-cation-proton-antiporters-in-vibrio-cholerae
#1
Muntahi Mourin, Carla B Schubiger, Craig T Resch, Claudia C Häse, Pavel Dibrov
The genome of Vibrio cholerae encodes three cation-proton antiporters of NhaP-type, Vc-NhaP1, 2, and 3. To examine physiological roles of Vc-NhaP antiporters, triple ΔnhaP1ΔnhaP2ΔnhaP3 and single ΔnhaP3 deletion mutants of V. cholerae were constructed and characterized. Vc-NhaP3 was, for the first time, cloned and biochemically characterized. Activity measurements on the inside-out membrane vesicle experimental model defined Vc-NhaP3 as a potassium-specific cation-proton antiporter. While elimination of functional Vc-NhaP3 resulted in only minor growth defect in potassium-rich medium at pH 6...
January 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28083716/manganese-supplementation-increases-adiponectin-and-lowers-icam-1-and-creatinine-blood-levels-in-zucker-type-2-diabetic-rats-and-downregulates-icam-1-by-upregulating-adiponectin-multimerization-protein-dsba-l-in-endothelial-cells
#2
Elodie Burlet, Sushil K Jain
Blood and tissue levels of manganese (Mn) are lower in type 2 diabetic and atherosclerosis patients compared with healthy subjects. Adiponectin has anti-diabetic and anti-atherogenic properties. Impairment in Disulfide bond A-like protein (DsbA-L) is associated with low adiponectin levels and diabetes. This study investigates the hypothesis that the beneficial effects of Mn supplementation are mediated by adiponectin and DsbA-L. At 6 weeks of age, Male Zucker diabetic fatty rats (ZDF) were randomly divided into two groups: diabetic controls and Mn-supplemented diabetic rats...
January 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28074342/the-presence-of-modified-nucleosides-in-extracellular-fluids-leads-to-the-specific-incorporation-of-5-chlorocytidine-into-rna-and-modulates-the-transcription-and-translation
#3
Caroline Noyon, Thierry Roumeguère, Cédric Delporte, Damien Dufour, Melissa Cortese, Jean-Marc Desmet, Christophe Lelubre, Alexandre Rousseau, Philippe Poelvoorde, Jean Nève, Luc Vanhamme, Karim Zouaoui Boudjeltia, Pierre Van Antwerpen
Myeloperoxidase (MPO) is able to promote several kinds of damage and is involved in mechanisms leading to various diseases such as atherosclerosis or cancers. An example of these damages is the chlorination of nucleic acids, which is considered as a specific marker of the MPO activity. Since 5-chlorocytidine has been recently shown in healthy donor plasmas, this study aimed at discovering if these circulating modified nucleosides could be incorporated into RNA and DNA and if their presence impacts the ability of enzymes involved in the incorporation, transcription, and translation processes...
January 10, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28074341/gap-junction-protein-connexin43-deregulation-contributes-to-bladder-carcinogenesis-via-targeting-mapk-pathway
#4
Xiao-Lin Ai, Qiang Chi, Yu Qiu, Hong-Yang Li, Dong-Jie Li, Jia-Xu Wang, Zhi-Yong Wang
High expression of connexins was found in a variety of cancers, but their role is still controversial. We investigated whether connexin43 (Cx43) contributed to bladder carcinogenesis through MAPK activation. In this study, we found that Cx43 expression was significantly increased in bladder cancer tissues and cell line. Overexpression of Cx43 in bladder cancer 5637 cells increased cell proliferation, promoted cell cycle progression, and inhibited apoptosis. Western blot showed that JNK and ERK pathways were dramatically activated in Cx43-overexpressed cells...
January 10, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28074340/clinical-significance-of-cathepsin-l-and-cathepsin-b-in-dilated-cardiomyopathy
#5
Siddharth Mehra, Manish Kumar, Mansi Manchanda, Ratnakar Singh, Bhaskar Thakur, Neha Rani, Sudheer Arava, Rajiv Narang, Dharamvir Singh Arya, Shyam S Chauhan
Dysregulated expression of lysosomal cysteine cathepsins is associated with adverse cardiac remodeling, a characteristic of several cardiovascular diseases. However, the information regarding the role of cysteine cathepsin L (CTSL) and cathepsin B (CTSB) in dilated cardiomyopathy (DCM) is limited. The present study was aimed to investigate the expression of CTSL and CTSB in animal model of doxorubicin (doxo)-induced cardiomyopathy as well as in peripheral blood samples of DCM patients. Cardiac tissue sections from doxo-treated and control rats were used to study the expression of CTSL and CTSB by enzyme assay and immunohistochemistry (IHC)...
January 10, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28070834/resveratrol-modulates-gsh-system-in-c6-astroglial-cells-through-heme-oxygenase-1-pathway
#6
Bernardo Assein Arús, Débora Guerini Souza, Bruna Bellaver, Diogo Onofre Souza, Carlos-Alberto Gonçalves, André Quincozes-Santos, Larissa Daniele Bobermin
Resveratrol is a dietary polyphenol that displays neuroprotective properties in several in vivo and in vitro experimental models, by modulating oxidative and inflammatory responses. Glutathione (GSH) is a key antioxidant in the central nervous system (CNS) that modulates several cellular processes, and its depletion is associated with oxidative stress and inflammation. Therefore, this study sought to investigate the protective effects of resveratrol against GSH depletion pharmacologically induced by buthionine sulfoximine (BSO) in C6 astroglial cells, as well as its underlying cellular mechanisms...
January 9, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28063007/oxygen-exchange-and-energy-metabolism-in-erythrocytes-of-rett-syndrome-and-their-relationships-with-respiratory-alterations
#7
Chiara Ciaccio, Donato Di Pierro, Diego Sbardella, Grazia Raffaella Tundo, Paolo Curatolo, Cinzia Galasso, Marta Elena Santarone, Maurizio Casasco, Paola Cozza, Alessio Cortelazzo, Marcello Rossi, Claudio De Felice, Joussef Hayek, Massimo Coletta, Stefano Marini
Rett syndrome (RTT) is a neurodevelopmental disorder, mainly affecting females, which is associated to a mutation on the methyl-CpG-binding protein 2 gene. In the pathogenesis and progression of classic RTT, red blood cell (RBC) morphology has been shown to be an important biosensor for redox imbalance and chronic hypoxemia. Here we have evaluated the impact of oxidation and redox imbalance on several functional properties of RTT erythrocytes. In particular, we report for the first time a stopped-flow measurement of the kinetics of oxygen release by RBCs and the analysis of the intrinsic affinity of the hemoglobin (Hb)...
January 7, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28063006/therapeutic-value-of-nerve-growth-factor-in-promoting-neural-stem-cell-survival-and-differentiation-and-protecting-against-neuronal-hearing-loss
#8
Zhao Han, Cong-Pin Wang, Ning Cong, Yu-Yan Gu, Rui Ma, Fang-Lu Chi
Nerve growth factor (NGF) is a neurotrophic factor that modulates survival and differentiation of neural stem cells (NSCs). We investigated the function of NGF in promoting growth and neuronal differentiation of NSCs isolated from mouse cochlear tissue, as well as its protective properties against gentamicin (GMC) ototoxicity. NSCs were isolated from the cochlea of mice and cultured in vitro. Effect of NGF on survival, neurosphere formation, and differentiation of the NSCs, as well as neurite outgrowth and neural excitability in the subsequent in vitro neuronal network, was examined...
January 7, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28063005/albendazole-inhibits-hif-1%C3%AE-dependent-glycolysis-and-vegf-expression-in-non-small-cell-lung-cancer-cells
#9
Fang Zhou, Jin Du, Jianjun Wang
Albendazole (ABZ) has an anti-tumor ability and inhibits HIF-1α activity. HIF-1α is associated with glycolysis and vascular endothelial cell growth factor (VEGF) expression, which plays an important role in cancer progression. These clues indicate that ABZ exerts an anti-cancer effect by regulating glycolysis and VEGF expression. The aim of this study is to clarify the effects of ABZ on non-small cell lung cancer (NSCLC) cells and explore the underlying molecular mechanisms. The expression levels of HIF-1α and VEGF were detected using western blot analysis, and the effect of ABZ on glycolysis was evaluated by measuring the relative activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH) and detecting the production of lactate in A549 and H1299 cells...
January 7, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28063004/tubulin-alpha-8-is-expressed-in-hepatic-stellate-cells-and-is-induced-in-transformed-hepatocytes
#10
Lisa Rein-Fischboeck, Rebekka Pohl, Elisabeth M Haberl, Sebastian Zimny, Maximilian Neumann, Kristina Eisinger, Thomas S Weiss, Sabrina Krautbauer, Christa Buechler
Tubulin alpha 8 (TUBA8) is highly abundant in murine liver tumors suggesting a role in hepatocellular carcinoma (HCC). Non-alcoholic steatohepatitis (NASH) is a risk factor for HCC. In mice that are fed with a methionine-choline deficient diet for two weeks to induce advanced murine NASH, we do see increased hepatic levels of TUBA8 protein. In animals given a high-fat diet for 14 weeks or an atherogenic diet for 12 weeks, hepatic TUBA8 is unchanged. TUBA8 is highly expressed in human hepatic stellate cells (HSC) and co-localizes with the HSC marker desmin in the murine liver...
January 7, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28063003/effect-of-poly-l-arginine-in-inhibiting-scrapie-prion-protein-of-cultured-cells
#11
Muhammad Waqas, Hye-Mi Lee, Jeeyoung Kim, Glenn Telling, Jin-Ki Kim, Dae-Hwan Kim, Chongsuk Ryou
Biological effect of poly-L-arginine (PLR), the linear homopolymer comprised of L-arginine, was investigated to determine the activity of suppressing prions. PLR decreased the level of scrapie prion protein (PrP(Sc)) in cultured cells permanently infected with prions in a concentration-dependent manner. The PrP(Sc) inhibition efficacy of PLR was greater than that of another prion-suppressant poly-L-lysine (PLK) in a molecular mass-dependent fashion. The effective concentration of PLR to inhibit prions was achieved safely below the cytotoxic concentrations, and overall cytotoxicity of PLR was similar to that of PLK...
January 7, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28039611/alternative-new-mesenchymal-stem-cell-source-exerts-tumor-tropism-through-alcam-and-n-cadherin-via-regulation-of-microrna-192-and-218
#12
Ran Kim, Sang In Park, Chang Youn Lee, Jihyun Lee, Pilseog Kim, Sekyung Oh, Hojin Lee, Min Young Lee, Jongmin Kim, Yong-An Chung, Ki-Chul Hwang, Lee-So Maeng, Woochul Chang
Gliomas are the most common type of malignant primary brain tumors. Some treatments of gliomas exist, but they are rarely curative. Mesenchymal stem cells (MSCs) are emerging as potential modes of targeted cancer therapy owing to their capacity for homing toward tumor sites. It has been proposed that MSCs derived from various sources, such as bone marrow, adipose tissue and umbilical cord blood, can be used as cell-based therapy for brain tumors. Here, MSCs obtained from the synovial fluid of osteoarthritis or rheumatoid arthritis patients were investigated as therapeutic candidates...
December 30, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28025797/inhibition-of-cell-survival-and-proliferation-by-nimbolide-in-human-androgen-independent-prostate-cancer-pc-3-cells-involvement-of-the-pi3k-akt-pathway
#13
Paulraj Raja Singh, Elayapillai Sugantha Priya, Solaimuthu Balakrishnan, Ramachandran Arunkumar, Govindaraj Sharmila, Manikkam Rajalakshmi, Jagadeesan Arunakaran
Prostate cancer is most common malignancy among men in the world. PI3K-Akt signaling appears to be critical to prostate cancer cell proliferation and survival. Our earlier study reveals that nimbolide (2 µM) prevents cell survival via IGF signaling pathway through PI3K/Akt and induces apoptosis in PC-3 cell line. Akt mediates the phosphorylation and activation of mTOR that plays a critical role in the regulation of protein translation and synthesis, angiogenesis, and cell cycle progression. The present study was aimed to investigate the effect of nimbolide on tPI3K, tAkt, pAkt, tmTOR, GSK3β, pGSK3β, PCNA, c-Myc, Cyclin D1, and Survivin protein levels by western blot analysis...
December 26, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28025796/ifn-%C3%AE-regulates-xanthine-oxidase-mediated-inos-independent-oxidative-stress-in-maneb-and-paraquat-treated-rat-polymorphonuclear-leukocytes
#14
Deepali Singh, Vinod Kumar, Chetna Singh
Maneb (MB) and paraquat (PQ) provoke oxidative stress-mediated cell damage. Role of xanthine oxidase (XO) in oxidative stress and its association with nitric oxide (NO)/NO synthase (NOS) have been widely reported. While inducible NOS (iNOS) is implicated in MB+PQ-induced toxicity in rat polymorphonuclear leukocytes (PMNs), role of XO and its alliance with iNOS have not yet been established. The study investigated the role of XO in MB+PQ-induced oxidative stress in rat PMNs and its regulation by iNOS and inflammatory cytokines...
December 26, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28025795/possible-role-of-hiwi2-in-modulating-tight-junction-proteins-in-retinal-pigment-epithelial-cells-through-akt-signaling-pathway
#15
Suganya Sivagurunathan, Karthikka Palanisamy, Jayamuruga Pandian Arunachalam, Subbulakshmi Chidambaram
PIWI subfamily of proteins is shown to be primarily expressed in germline cells. They maintain the genomic integrity by silencing the transposable elements. Although the role of PIWI proteins in germ cells has been documented, their presence and function in somatic cells remains unclear. Intriguingly, we detected all four members of PIWI-like proteins in human ocular tissues and somatic cell lines. When HIWI2 was knocked down in retinal pigment epithelial cells, the typical honeycomb morphology was affected...
December 26, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28013477/inhibition-of-pro-active-mmp-2-by-green-tea-catechins-and-prediction-of-their-interaction-by-molecular-docking-studies
#16
Animesh Chowdhury, Suman Kumar Nandy, Jaganmay Sarkar, Tapati Chakraborti, Sajal Chakraborti
Matrix metalloproteinases (MMPs) play a crucial role in developing different types of lung diseases, e.g., pulmonary arterial hypertension (PAH). Green tea polyphenolic catechins such as EGCG and ECG have been shown to ameliorate various types of diseases including PAH. Our present study revealed that among the four green tea catechins (EGCG, ECG, EC, and EGC), EGCG and ECG inhibit pro-/active MMP-2 activities in pulmonary artery smooth muscle cell (PASMC) culture supernatant. Based on the above, we investigated the interactions of pro-/active MMP-2 with the green tea catechins by computational methods...
December 24, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28012015/modulation-of-cell-death-in-human-colorectal-and-breast-cancer-cells-through-a-manganese-chelate-by-involving-gsh-with-intracellular-p53-status
#17
Kaushik Banerjee, Satyajit Das, Saikat Majumder, Subrata Majumdar, Jaydip Biswas, Soumitra Kumar Choudhuri
Chemotherapy is central to current treatment modality especially for advanced and metastatic colorectal and breast cancers. Targeting the key molecular events of the neoplastic cells may open a possibility to treat cancer. Although some improvements in understanding of colorectal and breast cancer treatment have been recorded, the involvement of glutathione (GSH) and dependency of p53 status on the modulation of GSH-mediated treatment efficacy have been largely overlooked. Herein, we tried to decipher the underlying mechanism of the action of Mn-N-(2-hydroxyacetophenone) glycinate (MnNG) against differential p53 status bearing Hct116, MCF-7, and MDA-MB-468 cells on the backdrop of intracellular GSH level and reveal the role of p53 status in modulating GSH-dependant abrogation of MnNG-induced apoptosis in these cancer cells...
December 23, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28012014/analysis-of-the-expression-of-human-bitter-taste-receptors-in-extraoral-tissues
#18
Appalaraju Jaggupilli, Nisha Singh, Jasbir Upadhyaya, Anurag S Sikarwar, Makoto Arakawa, Shyamala Dakshinamurti, Rajinder P Bhullar, Kangmin Duan, Prashen Chelikani
The 25 bitter taste receptors (T2Rs) in humans perform a chemosensory function. However, very little is known about the level of expression of these receptors in different tissues. In this study, using nCounter gene expression we analyzed the expression patterns of human TAS2R transcripts in cystic fibrosis bronchial epithelial (CuFi-1), normal bronchial epithelial (NuLi-1), airway smooth muscle (ASM), pulmonary artery smooth muscle (PASM), mammary epithelial, and breast cancer cells. Our results suggest a specific pattern of TAS2R expression with TAS2R3, 4, 5, 10, 13, 19, and 50 transcripts expressed at moderate levels and TAS2R14 and TAS2R20 (or TASR49) at high levels in the various tissues analyzed...
December 23, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28004351/withania-somnifera-as-a-potential-anxiolytic-and-immunomodulatory-agent-in-acute-sleep-deprived-female-wistar-rats
#19
Taranjeet Kaur, Harpal Singh, Rachana Mishra, Shaffi Manchanda, Muskan Gupta, Vedangana Saini, Anuradha Sharma, Gurcharan Kaur
Sleep is a profound regulator of cellular immunity, and the curtailment of sleep in present day lifestyle leads to disruption of neuro-immune-endocrine interactions. No therapeutic remedy is yet known for the amelioration of detrimental effects caused by sleep deprivation (SD). The current study was aimed to elucidate the effects of acute SD on immune function and its modulation by water extract from leaves of Withania somnifera (ASH-WEX). Three groups of animals, i.e. Vehicle-Undisturbed sleep (VUD), Vehicle-Sleep deprived (VSD) and ASH-WEX fed sleep deprived (WSD) rats were tested for their anxiety-like behaviour and further used for the study of inflammatory and apoptotic markers expression in piriform cortex and hippocampus regions of the brain...
December 21, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28004350/identification-of-an-rna-aptamer-binding-htert-derived-peptide-and-inhibiting-telomerase-activity-in-mcf7-cells
#20
Akhil Varshney, Jyoti Bala, Baby Santosh, Ashima Bhaskar, Suresh Kumar, Pramod K Yadava
Human telomerase reverse transcriptase is an essential rate-limiting component of telomerase complex. hTERT protein in association with other proteins and the human telomerase RNA (hTR) shows telomerase activity, essential for maintaining genomic integrity in proliferating cells. hTERT binds hTR through a decapeptide located in the RID2 (RNA interactive domain 2) domain of N-terminal region. Since hTERT is essential for telomerase activity, inhibitors of hTERT are of great interest as potential anti-cancer agent...
December 21, 2016: Molecular and Cellular Biochemistry
journal
journal
23110
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"