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Molecular and Cellular Biochemistry

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https://www.readbyqxmd.com/read/29790115/n-2-hydroxyphenyl-acetamide-and-its-gold-nanoparticle-conjugation-prevent-glycerol-induced-acute-kidney-injury-by-attenuating-inflammation-and-oxidative-injury-in-mice
#1
Rehan Ahmed Siddiqui, Shabana Usman Simjee, Nurul Kabir, Muhammad Ateeq, M Raza Shah, Syed Saad Hussain
The protective activity of N-(2-hydroxyphenyl)acetamide (NA-2) and NA-2-coated gold nanoparticles (NA-2-AuNPs) in glycerol-treated model of acute kidney injury (AKI) in mice was investigated. NA-2 (50 mg/kg) and NA-2-AuNPs (30 mg/kg) were given to the animals for four days followed by 24-h water deprivation and injection of 50% glycerol (10 ml/kg im). The animals were sacrificed on the next day. Blood and kidneys were collected for biochemical investigations (urea and creatinine), histological studies (hematoxylin and eosin; and periodic acid-Schiff staining), immunohistochemistry (actin and cyclooxygenase-2, Cox-2), and real-time RT-PCR (inducible nitric oxide synthase, iNOS; nuclear factor-κB p50, NFκB; hemeoxygenase-1, HO-1; and kidney injury molecule-1, Kim-1)...
May 22, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29790114/ivabradine-improved-left-ventricular-function-and-pressure-overload-induced-cardiomyocyte-apoptosis-in-a-transverse-aortic-constriction-mouse-model
#2
Yihui Yu, Zuoying Hu, Bing Li, Zhimei Wang, Shaoliang Chen
This study aimed to investigate the effects and molecular mechanisms of ivabradine in preventing cardiac hypertrophy in an established transverse aortic constriction (TAC) mouse model. A total of 56 male C57BL/6 mice were randomly assigned into the following seven groups (8 mice per group): sham, TAC model, Iva-10 (10 mg/kg/day ivabradine), Iva-20 (20 mg/kg/day ivabradine), Iva-40 (40 mg/kg/day ivabradine), Iva-80 (80 mg/kg/day ivabradine), and Rap (rapamycin, a positive control). Echocardiography and left ventricular hemodynamics were performed...
May 22, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29786764/encapsulation-of-micronutrients-resveratrol-genistein-and-curcumin-by-folic-acid-pamam-nanoparticles
#3
P Chanphai, H A Tajmir-Riahi
It has been shown that encapsulation of dietary polyphenols leads to increased solubility and bioavailability of these micronutrients. The encapsulation of dietary polyphenols resveratrol, genistein, and curcumin by folic acid-PAMAM-G3 and folic acid-PAMAM-G4 nanoparticles was studied in aqueous solution at physiological conditions, using multiple spectroscopic methods, TEM images, and docking studies. The polyphenol bindings are via hydrophilic, hydrophobic, and H-bonding contacts with resveratrol forming more stable conjugates...
May 21, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29786763/mir-124-promotes-proliferation-and-neural-differentiation-of-neural-stem-cells-through-targeting-dact1-and-activating-wnt-%C3%AE-catenin-pathways
#4
Shujie Jiao, Yaling Liu, Yaobing Yao, Junfang Teng
Neural stem cells (NSCs) are multipotent and undifferentiated cells with the potential to differentiate into neuronal lineages and gliocytes. NSCs have the ability to generate and regenerate the brain, indicating the possibility of cell-based therapies for neurological disorders. miR-124 has been demonstrated as a modulator in the survival, expansion, and differentiation of NSCs. However, the underlying molecular mechanisms of miR-124 in NSC development are still far from being understood. The expressions of miR-124, dishevelled binding antagonist of beta-catenin 1 (DACT1), ki-67, Nestin, β-tubulin III, glial fibrillary acidic protein (GFAP), β-catenin, cyclinD1, and glycogen synthase kinase-3β (GSK-3β) were examined by qRT-PCR or western blot...
May 21, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29777335/a-trisubstituted-pyrazole-derivative-reduces-dmba-induced-mammary-tumor-growth-in-rats-by-inhibiting-estrogen-receptor-%C3%AE-expression
#5
Hanumappa Ananda, Kothanahally S Sharath Kumar, Muddenahalli S Sudhanva, Shobith Rangappa, Kanchugarakoppal S Rangappa
Aberrant expression of estrogen receptor alpha (ER-α) is observed in many pathological complications like breast cancer, endometrial cancer, and in osteoporosis. ER-α plays a vital role in the initiation and progression of breast cancer and confers chemo and radioresistance to the cancer cells by upregulating expression of anti-apoptotic proteins. The synthetic pyrazole derivative 3-(1-(4-bromophenyl)-5-phenyl-1H-pyrazol-3-yl)pyridine (compound 5d) displays significant cytotoxicity against mammary carcinoma cells...
May 18, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29761248/biglycan-promotes-the-chemotherapy-resistance-of-colon-cancer-by-activating-nf-%C3%AE%C2%BAb-signal-transduction
#6
Bin Liu, Tonghong Xu, Xinning Xu, Yuzhu Cui, Xiaojing Xing
Biglycan (BGN) is overexpressed in cancer stem cells of colon cancer and induces the activation of NF-κB pathway which contributes to the chemotherapy resistance of diverse cancer types. Therefore, we hypothesized that the overexpression of BGN also promoted the development of multiple drug resistance (MDR) in colon cancer via NF-κB pathway. The expression of BGN was bilaterally modulated in colon cancer cell lines HT-29 and SW-480 and the effect of treatments on the cell proliferation and resistance to 5-FU was assessed...
May 14, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29761247/beneficial-effects-of-fenofibrate-in-pulmonary-hypertension-in-rats
#7
Palak Galhotra, Pankaj Prabhakar, Himanshu Meghwani, Soheb A Mohammed, Sanjay Kumar Banerjee, Sandeep Seth, Milind P Hote, K H Reeta, Ruma Ray, Subir Kumar Maulik
Pulmonary hypertension (PH) is a morbid complication of cardiopulmonary as well as several systemic diseases in humans. It is rapidly progressive and fatal if left untreated. In the present study, we investigated the effect of PPARα agonist fenofibrate (FF) on monocrotaline (MCT)-induced PH in rats. FF, because of its pleiotropic property, could be helpful in reducing inflammation, oxidative stress, and reactive oxygen species. On day 1, MCT (50 mg/kg, s.c.) was given to all the rats in MCT, sildenafil, and FF group except normal control rats...
May 14, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29737449/modification-of-gene-expression-profiling-related-to-renin-angiotensin-system-in-an-ischemia-reperfusion-rat-model-after-t3-infusion
#8
Laura Sabatino, Silvana Balzan, Claudia Kusmic, Giorgio Iervasi
Triiodothyronine (T3) and renin-angiotensin system (RAS) are functionally related in cardiovascular system. Recently, in an in vivo myocardial ischemia/reperfusion (I/R) model in rats, we showed that T3 treatment improved the post-ischemic recovery of cardiac function. In the present study, we used the same experimental model of regional I/R, obtained by 30 min occlusion of the left descending coronary artery, followed by 3-days of reperfusion, to investigate the effect of 48-h treatment (started 1 day after ischemia) with 6 µg/kg/day T3 or vehicle...
May 8, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29725980/correction-to-jip3-localises-to-exocytic-vesicles-and-focal-adhesions-in-the-growth-cones-of-differentiated-pc12-cells
#9
Patrick T Caswell, Martin Dickens
The article "JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells", written by "Patrick T. Caswell, Martin Dickens", was originally published electronically on the publisher's internet portal https://link.springer.com/article/10.1007/s11010-017-3222-7 on 20 November 2017 without open access.
May 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29700729/possible-use-of-mirnas-146a-and-499-expression-and-their-polymorphisms-as-diagnostic-markers-for-rheumatoid-arthritis
#10
Ghada Ayeldeen, Yasser Nassar, Hanan Ahmed, Olfat Shaker, Tamer Gheita
Rheumatoid arthritis (RA) is a systemic autoimmune disorder affecting the peripheral joints. Different microRNAs had been investigated in RA including miRNA-146a meanwhile, miRNA-499 there were no studies to prove its expression in RA serum samples. This study was performed to investigate expression of both miRNAs-146a and -499 and their polymorphisms in Egyptian patients with RA and to evaluate their relationship with clinico-pathological data. The present study includes 108 subjects classified into two main groups: 52 RA patients and 56 unrelated healthy controls...
April 26, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29696609/apocynin-protects-endothelial-cells-from-endoplasmic-reticulum-stress-induced-apoptosis-via-ire1%C3%AE-engagement
#11
Jie Wu, Weijin Zhang, Xiaohui Liu, Lili Wu, Guangting He, Peixin Li, Xiaohua Guo, Zhongqing Chen, Qiaobing Huang
Endoplasmic reticulum (ER) stress-induced endothelial cell (EC) apoptosis has been implicated in a variety of human diseases. In addition to being regarded as an NADPH oxidase (NOX) inhibitor, apocynin (APO) exhibits an anti-apoptotic effect in various cells. The present study aimed to identify the protective role of apocynin in ER stress-mediated EC apoptosis and the underlying mechanisms. We found that ER stress resulted in a significant increase in c-Jun N-terminal kinase phosphorylation, and elicited caspase 3 cleavage and apoptosis...
April 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29696608/regulation-of-aging-and-oxidative-stress-pathways-in-aged-pancreatic-islets-using-alpha-lipoic-acid
#12
Navid Nobakht-Haghighi, Mahban Rahimifard, Maryam Baeeri, Mohammad Amin Rezvanfar, Shermineh Moini Nodeh, Hamed Haghi-Aminjan, Emre Hamurtekin, Mohammad Abdollahi
Oxidative stress has been involved in the aging process and the pathogenesis of type-2 diabetes, which is a serious health problem worldwide. This study investigates the anti-aging, anti-apoptotic, and antioxidant properties of alpha-lipoic acid (ALA), aiming to improve aged rat pancreatic cells. In this regard, half maximal effective concentration (EC50 ) of ALA based on the survival of aged pancreatic islet cells was determined as 100 µM. Following this, p38 and p53 genes expression as key factors in aging, oxidative stress biomarkers, insulin secretion, and Pdx1 protein expression were evaluated using real-time PCR, ELISA reader, and fluorescence microscope...
April 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29679279/role-of-inositol-polyphosphates-in-programed-cell-death-in-dictyostelium-discoideum-and-its-developmental-life-cycle
#13
Qudes Al-Anbaky, Zeiyad Al-Karakooly, Richard Connor, Lisa Williams, Azure Yarbrough, John Bush, Nawab Ali
Programed cell death or apoptosis is a key developmental process that maintains tissue homeostasis in multicellular organisms. Inositol polyphosphates (InsPs) are key signaling molecules known to regulate a variety of cellular processes including apoptosis in such organisms. The signaling role of InsPs in unicellular organisms such as Dictyostelium discoideum (D. discoideum) is not well understood. We investigated whether InsPs also play any role in apoptosis in D. discoideum and whether InsPs-mediated apoptosis follows a mechanism similar to that present in higher multicellular eukaryotes...
April 20, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29679278/overexpression-of-modified-human-tr%C3%AE-1-suppresses-the-growth-of-hepatocarcinoma-sk-hep1-cells-in-vitro-and-in-xenograft-models
#14
Xiaoxiang Peng, Yuntao Zhou, Yanli Sun, Wei Song, Xiangying Meng, Chunling Zhao, Ronglan Zhao
Association studies suggest that TRβ1 functions as a tumor suppressor. Thyroid hormone receptors (TRs) mediate transcriptional responses through a highly conserved DNA-binding domain (DBD). We previously constructed an artificially modified human TRβ1 (m-TRβ1) via the introduction of a 108-bp exon sequence into the corresponding position of the wild-type human TRβ1 (TRβ1) DBD. Studies confirmed that m-TRβ1 was functional and could inhibit the proliferation of breast cancer MDA-MB-468 cells in vitro. To understand the role of m-TRβ1 in liver tumor development, we adopted a gain-of-function approach by stably expressing TRβ (m-TRβ1 and TRβ1) genes in a human hepatocarcinoma cell line, SK-hep1 (without endogenous TRβ), and then evaluated the effects of the expressed TRβ on cancer cell proliferation, migration, and tumor growth in cell-based studies and xenograft models...
April 20, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29679277/the-role-of-mitochondrial-dna-damage-at-skeletal-muscle-oxidative-stress-on-the-development-of-type-2-diabetes
#15
Julia Matzenbacher Dos Santos, Denise Silva de Oliveira, Marcos Lazaro Moreli, Sandra Aparecida Benite-Ribeiro
Reduced cellular response to insulin in skeletal muscle is one of the major components of the development of type 2 diabetes (T2D). Mitochondrial dysfunction involves in the accumulation of toxic reactive oxygen species (ROS) that leads to insulin resistance. The aim of this study was to verify the involvement of mitochondrial DNA damage at ROS generation in skeletal muscle during development of T2D. Wistar rats were fed a diet containing 60% fat over 8 weeks and at day 14 a single injection of STZ (25 mg/kg) was administered (T2D-induced)...
April 20, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29675630/saikosaponin-d-inhibits-proliferation-by-up-regulating-autophagy-via-the-camkk%C3%AE-ampk-mtor-pathway-in-adpkd-cells
#16
Weiwei Shi, Dechao Xu, Junhui Gu, Cheng Xue, Bo Yang, Lili Fu, Shuwei Song, Dongmei Liu, Wei Zhou, Jiayi Lv, Ke Sun, Meihan Chen, Changlin Mei
Autosomal dominant polycystic kidney disease (ADPKD) is a common heritable human disease. Recently, the role of repressed autophagy in ADPKD has drawn increasing attention. Here, we investigate the mechanism underlying the effect of Saikosaponin-d (SSd), a sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump (SERCA) inhibitor. We show that SSd suppresses proliferation in ADPKD cells by up-regulating autophagy. We found that treatment with SSd results in the accumulation of intracellular calcium, which in turn activates the CaMKKβ-AMPK signalling cascade, inhibits mTOR signalling and induces autophagy...
April 19, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29671254/co-enzyme-q10-upregulates-hsp70-and-protects-chicken-primary-myocardial-cells-under-in-vitro-heat-stress-via-pkc-mapk
#17
Jiao Xu, Shu Tang, Bin Yin, Jiarui Sun, Endong Bao
In this report, we investigate the protective mechanism of co-enzyme Q10 on chicken primary myocardial cells during heat stress. Morphological observations indicate that addition of co-enzyme Q10 protects myocardial cells from heat stress, reduces the damage of mitochondria and nucleus, and decreases the mean number of vacuolated mitochondria. We have previously shown that co-enzyme Q10 can protect myocardial cells by upregulating the expression of Hsp70. Therefore, signaling pathways involved in this process were explored...
April 18, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29667111/propofol-specifically-suppresses-il-1%C3%AE-secretion-but-increases-bacterial-survival-in-staphylococcus-aureus-infected-raw264-7-cells
#18
Ming-Shan Chen, Wen-Chun Lin, Hsuan-Te Yeh, Chia-Lin Hu, Shew-Meei Sheu
Anesthetics have immunomodulatory effects, but the use of different assay systems has contributed to inconsistent results in the literature. IL-1β and reactive oxygen species (ROS) secreted by phagocytes are important factors that protect against Staphylococcus aureus infection. In this study, the effects of four intravenous anesthetics (propofol, thiamylal sodium, midazolam, and ketamine) on IL-1β secretion, ROS, and bacterial survival in S. aureus-infected RAW264.7 cells were evaluated. S. aureus-infected RAW264...
April 17, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29644529/effects-of-increased-accumulation-of-doxorubicin-due-to-emodin-on-efflux-transporter-and-lrp1-expression-in-lung-adenocarcinoma-and-colorectal-carcinoma-cells
#19
Vidhya V Iyer, P Yoga Priya, Jeipreeti Kangeyavelu
Treatment with doxorubicin (dox) and emodin, separately and together, under normoxic and hypoxia-like conditions induced by CoCl2 , led to greater intracellular compound accumulation over 10 h post-addition in the presence of CoCl2 in lung adenocarcinoma (A549) and colorectal carcinoma (HCT-15) cell lines. Confocal microscopy revealed that emodin, by itself, showed high cytosolic distribution in both cell lines, at 40 min post-addition but had entered the nuclei by 2 h, while dox entered the nuclei by 40 min...
April 11, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29644528/a-novel-semi-synthetic-diterpenoid-16-r-and-s-phenylamino-cleroda-3-13-14-z-dien-15-16-olide-pgea-an-inhibits-the-growth-and-cell-survival-of-human-neuroblastoma-cell-line-sh-sy5y-by-modulating-p53-pathway
#20
Syed Saad Hussain, Kinza Rafi, Shaheen Faizi, Zaid Abdul Razzak, Shabana U Simjee
Neuroblastoma being the most common extracranial pediatric solid tumor accounts for 15% of overall cancer-related childhood mortalities. Resistance to chemotherapeutic drugs is one of the limiting factors for positive prognosis for neuroblastoma. Therefore, there is always a need for developing new therapeutic moieties which can become a future prospect of neuroblastoma therapy. Terpenoids being the largest natural compounds have demonstrated many biological activities including anticancer activity. Keeping in mind the role of terpenoids in biological system, we aimed to identify novel semi-synthetic terpenoid derived from cleroda diterpene, 16-oxo-cleroda-3,13(14)E-diene-15-oic acid (1) as a potential anticancer moiety against neuroblastoma...
April 11, 2018: Molecular and Cellular Biochemistry
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