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Molecular and Cellular Biochemistry

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https://www.readbyqxmd.com/read/30014221/structural-effects-of-methylglyoxal-glycation-a-study-on-the-model-protein-mnei
#1
Serena Leone, Jole Fonderico, Chiara Melchiorre, Andrea Carpentieri, Delia Picone
The reaction of free amino groups in proteins with reactive carbonyl species, known as glycation, leads to the formation of mixtures of products, collectively referred to as advanced glycation endproducts (AGEs). These compounds have been implicated in several important diseases, but their role in pathogenesis and clinical symptoms' development is still debated. Particularly, AGEs are often associated to the formation of amyloid deposits in conformational diseases, such as Alzheimer's and Parkinson's disease, and it has been suggested that they might influence the mechanisms and kinetics of protein aggregation...
July 16, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30014220/the-utrophin-beta-2-syntrophin-complex-regulates-adipocyte-lipid-droplet-size-independent-of-adipogenesis
#2
Sabrina Krautbauer, Markus Neumeier, Elisabeth M Haberl, Rebekka Pohl, Susanne Feder, Kristina Eisinger, Lisa Rein-Fischboeck, Christa Buechler
Utrophin is a widely expressed cytoskeleton protein and is associated with lipid droplets (LDs) in adipocytes. The scaffold protein beta 2 syntrophin (SNTB2) controls signaling events by recruiting distinct membrane and cytoskeletal proteins, and binds to utrophin. Here we show that SNTB2 forms a complex with utrophin in adipocytes. SNTB2 protein is strongly diminished when utrophin is low. Of note, knock-down of utrophin or SNTB2 enhances LD growth during adipogenesis. SNTB2 reduction has no effect on basal and induced lipolysis, and insulin-stimulated phosphorylation of Akt is normal...
July 16, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30008033/effects-of-insulin-like-growth-factor-1-on-endoplasmic-reticulum-stress-and-autophagy-in-rat-gastric-smooth-muscle-cells-cultured-at-different-glucose-concentrations-in-vitro
#3
Xue-Sen Fang, Mo-Han Zhang, Jun-Yu Guo, Zheng Jin
The purpose of the study was to observe changes in endoplasmic reticulum stress (ERS)- and autophagy-related proteins in gastric smooth muscle tissues of diabetic rats with gastroparesis, investigate the effect of insulin-like growth factor 1 (IGF-1) on ERS and autophagy in rat gastric smooth muscle cells cultured under different glucose concentrations, and explore the influence of IGF-1 on development of diabetic gastroparesis (DGP). After establishing a rat model of DGP, rats were divided into normal control (NC) and 6-week diabetic model (DM6W) groups...
July 14, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30003444/insulin-dependent-glucose-transporter-1-mediated-glucose-uptake-and-tube-formation-in-the-human-placental-first-trimester-trophoblast-cells
#4
Sanjay Basak, Srinivas Vilasagaram, Kishore Naidu, Asim K Duttaroy
During early gestation, hypoxic condition is critically maintained by optimal glucose metabolism and transporter activities. Glucose is readily available energy nutrient required for placentation. However, limited data are available on glucose uptake and its transporters during first trimester placentation processes. To this end, effects of glucose and the roles of glucose transporters (GLUTs) were investigated during hypoxia on trophoblast migration and placental angiogenesis processes using early gestation-derived trophoblast cells, HTR8/SVneo, and first trimester human placental explant tissues...
July 12, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29995265/anti-neuroinflammatory-effects-of-galangin-in-lps-stimulated-bv-2-microglia-through-regulation-of-il-1%C3%AE-production-and-the-nf-%C3%AE%C2%BAb-signaling-pathways
#5
Mi Eun Kim, Pu Reum Park, Ju Yong Na, Inae Jung, Jun Hwi Cho, Jun Sik Lee
Neuroinflammation resulting from microglial activation is involved in the pathogenesis of neurodegenerative diseases, including Parkinson's diseases. Microglial activation plays an important role in neuroinflammation and contributes to several neurological disorders. Hence, inhibition of both microglial activation and the generation of pro-inflammatory cytokines may lead to an effective treatment for neurodegenerative diseases. In the present study, the anti-neuroinflammatory effects of galangin were investigated in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells...
July 11, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29992460/fam98a-is-localized-to-stress-granules-and-associates-with-multiple-stress-granule-localized-proteins
#6
Kanako Ozeki, Mai Sugiyama, Khondker Ayesha Akter, Kimitoshi Nishiwaki, Eri Asano-Inami, Takeshi Senga
Stress granules are evolutionally conserved ribonucleoprotein structures that are formed in response to various stress stimuli. Recent studies have demonstrated that proteins with low complexity (LC) regions play a critical role for the formation of stress granules. In this study, we report that FAM98A, whose biological functions are unknown, is a novel component of stress granules. FAM98A is localized to stress granules, but not to P-bodies, after various stress stimuli. Analysis with deletion mutants revealed that C-terminal region that contains LC region was essential for FAM98A accumulation to stress granules...
July 10, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29980883/anti-inflammatory-and-anticancer-effects-of-p-methoxycinnamic-acid-an-active-phenylpropanoid-against-1-2-dimethylhydrazine-induced-rat-colon-carcinogenesis
#7
Sivagami Gunasekaran, Karthikkumar Venkatachalam, Nalini Namasivayam
p-methoxycinnamic acid (p-MCA) is an active phenolic acid found in rice bran, turmeric, brown rice, Kaempferia galanga, buckwheat inflorescence, etc. Earlier, we have reported that p-methoxycinnamic acid possesses antioxidant and antilipidperoxidative effects on 1,2-dimethylhyrdrazine (DMH)-induced colon carcinogenesis. The purpose of this study is to unravel the anti-inflammatory and anticancer properties of p-MCA against DMH-induced colon carcinogenesis. Male albino Wistar rats were randomly divided into six groups...
July 6, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29971544/attenuation-of-type-1-diabetes-induced-cardiovascular-dysfunctions-by-direct-thrombin-inhibitor-in-rats-a-mechanistic-study
#8
Yogesh Bulani, Krishnamoorthy Srinivasan, Shyam Sunder Sharma
Chronic diabetes is associated with ventricular dysfunctions in the absence of hypertension and coronary artery diseases. This condition is termed as diabetic cardiomyopathy (DCM). There is no favourable treatment available for the management of diabetic cardiomyopathy. Recent studies have reported increase in circulating thrombin level among diabetic patients which is responsible for hypercoagulability of blood. Thrombin induces inflammation and fibrosis, and enhances cardiac cell growth and contractility in vitro...
July 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29971543/maresin-1-attenuates-pro-inflammatory-reactions-and-er-stress-in-huvecs-via-ppar%C3%AE-mediated-pathway
#9
Tae Woo Jung, Hyung Sub Park, Geum Hee Choi, Daehwan Kim, Sung Ho Ahn, Dong-Seok Kim, Taeseung Lee, Ji Hoon Jeong
The current study was designed to investigate the therapeutic effects of Maresin 1 (MAR1) on atherosclerotic response. Human monocytes THP-1 and human umbilical vein endothelial cells (HUVECs) were used to investigate the effects of MAR1 on lipopolysaccharide (LPS)-induced inflammation and apoptosis. In this study, we found that MAR1 induces peroxisome proliferator-activated receptor alpha (PPARα) expression. We also demonstrated that MAR1 suppresses atherosclerotic reactions caused by LPS treatment via a PPARα-dependent pathway...
July 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29968167/-i-3-ii-3-biacacetin-mediated-cell-death-involves-mitochondria
#10
Jyotsna Nambiar, Gayathri Vijayakumar, G Drishya, Sanu K Shaji, Nanjan Pandurangan, Geetha B Kumar, Bipin G Nair
Dysregulation of the dynamic balance between cell proliferation and cell death leads to several malignancies including cancer. Biflavones are known to possess anti-proliferative activity against numerous cancer cell lines. The current study was undertaken to understand the mechanism of action of the biflavonoid (I-3,II-3)-biacacetin on MDA-MB-231. Biacacetin induces dose-dependent cell death in MDA-MB-231 cells from concentrations as low as 0.5 μM, which was further confirmed by an increase in sub-G1 cells...
July 2, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29968166/response-of-dna-damage-genes-in-acrolein-treated-lung-adenocarcinoma-cells
#11
Poonam Sarkar
Acrolein is a α-β-unsaturated aldehyde and is toxic to human upon its exposure from the environment. Sources of exposure to acrolein can be from heating cooking oil, automobile exhaust, tobacco smoke, and plastic waste. Acrolein exposure to lung is a major concern because of its volatile nature and due to its presence in the urban atmospheric air. Acrolein being highly reactive forms DNA and protein adducts, thereby making the cells vulnerable to long-term damage. Such long-term effect can lead to high susceptibility towards malignant transformation as has been reported in cigarette smokers...
July 2, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29961211/basement-membrane-extract-attenuates-the-more-malignant-gene-expression-profile-accentuated-by-fibronectin-in-prostate-cancer-cells
#12
Bruno Martinucci, Brenda de Carvalho Minatel, Maira Smaniotto Cucielo, Mariana Medeiros, Ivan José Vechetti-Junior, Sérgio Luis Felisbino, Flávia Karina Delella
Prostate cancer (PCa) has high mortality rates, with most of the deaths resulting from the development of metastasis. Fibronectin (FN) plays key roles in cell adhesion and affects the migratory behavior of cells. In the tumor microenvironment and also in the blood plasma during metastasis, FN displays increased expression, however its role in prostate cancer remains poorly understood. This study aimed to unveil the specific roles of FN as a soluble component, alone or in combination with a complex basement membrane...
June 30, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29961210/is-there-any-evidence-that-age-srage-is-a-universal-biomarker-risk-marker-for-diseases
#13
REVIEW
Kailash Prasad
Advanced glycation end products (AGE) and its cell-bound receptor called receptor for AGE (RAGE) are implicated in the pathogenesis of numerous diseases. Soluble receptor for AGE (sRAGE) counteracts the adverse effects of AGE-RAGE interaction by competing with RAGE for binding with AGE. Low levels of serum sRAGE have been proposed as a biomarker for diseases. However, the serum levels of sRAGE in diabetes and end-stage renal disease (ESRD) are elevated. Thus, low levels of sRAGE cannot be a universal biomarker...
June 30, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29959592/mir-377-3p-regulates-adipogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-by-regulating-lifr
#14
Xingnuan Li, Yaofang Yang, Ruiqiao Yan, Xiaoyuan Xu, Liyun Gao, Jun Mei, Jianyun Liu, Xinping Wang, Jie Zhang, Ping Wu, Weidong Li, Zhijun Zhao, Jianjun Xiong, Tao Wang
MicroRNAs are members of the family of non-coding small RNAs that regulate gene expression either by inhibiting mRNA translation or by promoting mRNA degradation at the post-transcriptional level. They play an important role in the differentiation of human bone marrow mesenchymal stem cells (hMSCs) into adipocytes. However, the role of microRNAs in this process remains to be poorly understood. Here, we observed that miR-377-3p expression was markedly decreased during adipogenic differentiation of hMSCs. Overexpression of miR-377-3p decreased adipocyte differentiation and downregulated the expression of adipogenic markers...
June 29, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29943371/proteomics-and-bioinformatics-analyses-identify-novel-cellular-roles-outside-mitochondrial-function-for-human-miro-gtpases
#15
Laura J Kay, Vartul Sangal, Gary W Black, Meera Soundararajan
The human Miro GTPases (hMiros) have recently emerged as important mediators of mitochondrial transport and may significantly contribute to the development of disorders such as Alzheimer's and schizophrenia. The hMiros represent two highly atypical members of the Ras superfamily, and exhibit several unique features: the presence of a GTPase domain at both the N-terminus and C-terminus, the presence of two calcium-binding EF-hand domains and localisation to the mitochondrial outer membrane. Here, elucidation of Miro GTPase signalling pathway components was achieved through the use of molecular biology, cell culture techniques and proteomics...
June 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29938378/hydrogen-peroxide-induced-oxidative-stress-and-its-impact-on-innate-immune-responses-in-lung-carcinoma-a549-cells
#16
Shishir Upadhyay, Saurabh Vaish, Monisha Dhiman
The immune responses, involved in recognition of cancer-specific antigens, are of particular interest as this may provide major leads towards developing new vaccines and antibody therapies against cancer. An effective treatment for cancer is still a challenge because there are many mechanisms through which the tumor cells can escape the host immune surveillance. Oxidative stress or respiratory burst which is host's mechanism to kill the foreign particles is used as defense mechanism by the tumor cells. The tumor cells uses this oxidative stress to form neo-antigens which in turn makes them undetectable and can escape the host immune surveillance...
June 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29936684/the-effects-of-l-cysteine-and-n-acetyl-l-cysteine-on-homocysteine-metabolism-and-haemostatic-markers-and-on-cardiac-and-aortic-histology-in-subchronically-methionine-treated-wistar-male-rats
#17
Sanja Kostić, Žarko Mićovic, Lazar Andrejević, Saša Cvetković, Aleksandra Stamenković, Sanja Stanković, Radmila Obrenović, Milica Labudović-Borović, Dragan Hrnčić, Vladimir Jakovljević, Dragan Djurić
Methionine is the precursor of homocysteine, a sulfur amino acid intermediate in the methylation and transsulfuration pathways; methionine-rich diets were used to induce hyperhomocysteinemia, and cardiovascular pathology was often observed. Other sulfur amino acids interfere with this metabolism, i.e., L-cysteine (Cys) and N-aceyl-L-cysteine (NAC), and probably also affect cardiovascular system. Their effects are controversial due to their ability to act both as anti- or pro-oxidant. Thus, this study aimed to elucidate their influence on levels of homocysteine, folate and vitamin B12, levels of different haemostatic parameters (fibrinogen, D-dimer, vWF Ag, vWF Ac) in rat serum or plasma as well as their effects on cardiac and aortic tissue histology in subchronically methionine-treated rats...
June 23, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29934862/label-free-quantitative-proteomics-analysis-reveals-distinct-molecular-characteristics-in-endocardial-endothelium
#18
Abdul Jaleel, A Aneesh Kumar, G S Ajith Kumar, Arun Surendran, Chandrashekaran C Kartha
Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally very important, no studies at protein level have been conducted so far characterizing endocardial endothelium. In this study, we used endothelial cells from pig heart to investigate if endocardial endothelial cells are distinct at the proteome level...
June 22, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29931518/biofunctionalized-self-assembly-of-peptide-amphiphile-induces-the-differentiation-of-bone-marrow-mesenchymal-stem-cells-into-neural-cells
#19
Hong Ruan, Renshun Xiao, Xinghai Jiang, Biao Zhao, Kai Wu, Zongzuan Shao, Zhongjie Zhang, Huyang Duan, Yulin Song
Bone marrow mesenchymal stem cells (BMSCs) are multipotential differentiation cells which can differentiate into different cell types such as osteoblasts, chondrocytes, adipocytes, cardiomyocytes, hepatocytes, endothelial cells, and neuronal cells. Such multipotential differentiation makes them attractive for stem cell-based therapy aimed at treating previously incurable disorders. In the present work, we encapsulated BMSCs into a hydrogel with a three-dimensional (3D) network of nanofibers, formed from self-assembling of peptide amphiphile...
June 21, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29926321/loss-of-the-basic-helix-loop-helix-transcription-factor-bhlhe41-induces-cell-death-and-impairs-neurite-outgrowth-in-neuro2a-cells
#20
Yajuan Sun, Haina Zhang, Libo Wang, Jia Li, Hui Jin, Zhenyu Wang, Shiyuan Tian, Ling Qi, Xiaoyang Liu
The basic helix-loop-helix (bHLH) superfamily of transcription factors have been implicated in a wide range of cellular functions such as proliferation, differentiation, tumorigenesis, and circadian rhythms. In a previous siRNA-based screen, bHLH family member e41 (BHLHE41) had been identified as a putative regulator of neuronal differentiation; however, its function remains largely elusive. To this end, using the CRISPR-Cas9 system, we established an isogenic Neuro2a (N2a) cell line with biallelic targeting of Bhlhe41 gene (Bhlhe41-/- )...
June 20, 2018: Molecular and Cellular Biochemistry
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