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Molecular and Cellular Biochemistry

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https://www.readbyqxmd.com/read/30194583/hydrogen-bond-linked-pathways-of-peptide-units-and-polar-groups-of-amino-acid-residues-suitable-for-electron-transfer-in-cytochrome-c-proteins
#1
T Ramasarma, D Vaigundan
Electron transfer occurs through heme-Fe across the cytochrome c protein. The current models of long range electron transfer pathways in proteins include covalent σ-bonds, van der Waals forces, and through space jump. Hydrogen-bond-linked pathways of delocalized electron units in peptide units and polar side chains of amino acid residues in proteins and internal water molecules are better suited for intramolecular atom-to-atom electron transfer in proteins. Crystal structures of cytochrome c proteins from horse (1HRC), tuna (3CYT), rice (1CCR), and yeast (3CX5) were analyzed using pymol software for 'Hydrogen Bonds' marking the polar atoms within the distance of 2...
September 7, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30194582/estradiol-attenuates-ischemia-reperfusion-induced-acute-kidney-injury-through-ppar-%C3%AE-stimulated-enos-activation-in-rats
#2
Amrit Pal Singh, Nirmal Singh, Devendra Pathak, Preet Mohinder Singh Bedi
We investigated the involvement of peroxisome proliferator activated receptor-γ (PPAR-γ)/endothelial nitric oxide synthase (eNOS) pathway in estradiol mediated protection against ischemia reperfusion (I/R)-induced acute kidney injury (AKI) in rats. To induce AKI, rats underwent 40 min of bilateral renal ischemia followed by 24 h of reperfusion. I/R-induced kidney damage was quantified by measuring serum creatinine, creatinine clearance, urea nitrogen, uric acid, potassium, fractional excretion of sodium, microproteinuria, and renal oxidative stress (thiobarbituric acid reactive substances, superoxide anion generation, and reduced glutathione)...
September 7, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30191481/lasalocid-immediately-and-completely-prevents-the-myocardial-damage-caused-by-coronary-ischemia-reperfusion-in-rat-heart
#3
Sergio F Estrada-Orihuela, Carlos Ibarra-Pérez
Lasalocid, a specific mobile membrane ionophore for calcium, dopamine and norepinephrine was assayed in its capacity to reduce or maintain unaltered the cardiovascular function in conditions of imminent myocardial injury. In experiments of coronary blockade and reperfusion carried out in rat heart, it was found that when administered from 5 to 30 minutes prior to the induction of coronary blockade, at a concentration of 2 mg/kg of body weight, the ionophore immediately, simultaneously, and completely interrupts the blood pressure decay, cardiac frequency increase, electrical ventricular tachycardia and fibrillation, as well as the fall of mitochondrial oxidative phosphorylation and decay of mitochondrial oxygen uptake provoked by the induced myocardial injury...
September 6, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30191480/the-mitochondrial-nod-like-receptor-nlrx1-modifies-apoptosis-through-sarm1
#4
Samuel A Killackey, Muhammed A Rahman, Fraser Soares, Ashley B Zhang, Mena Abdel-Nour, Dana J Philpott, Stephen E Girardin
NLRX1, the mitochondrial NOD-like receptor (NLR), modulates apoptosis in response to both intrinsic and extrinsic cues. Insights into the mechanism of how NLRX1 influences apoptosis remain to be determined. Here, we demonstrate that NLRX1 associates with SARM1, a protein with a toll/interleukin-1 receptor (TIR)-containing domain also found in adaptor proteins downstream of toll-like receptors, such as MyD88. While a direct role of SARM1 in innate immunity is unclear, the protein plays essential roles in Wallerian degeneration (WD), a type of neuronal catabolism occurring following axonal severing or damage...
September 6, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30178275/induction-of-hrr-genes-and-inhibition-of-dnmt1-is-associated-with-anthracycline-anti-tumor-antibiotic-tolerant-breast-carcinoma-cells
#5
Hemantika Dasgupta, Md Saimul Islam, Neyaz Alam, Anup Roy, Susanta Roychoudhury, Chinmay Kumar Panda
The aim of the study was to understand the role of homologous recombination repair (HRR) pathway genes in development of chemotolerance in breast cancer (BC). For this purpose, chemotolerant BC cells were developed in MCF-7 and MDA MB 231 cell lines after treatment with two anthracycline anti-tumor antibiotics doxorubicin and nogalamycin at different concentrations for 48 h with differential cell viability. The drugs were more effective in MCF-7 (IC50: 0.214-0.242 µM) than in MDA MB 231 (IC50: 0.346-0.37 µM) as shown by cell viability assay...
September 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30178274/stimulation-role-of-epinephrine-in-transcription-of-the-melatonin-synthesis-key-enzyme-aanat-in-the-pineal-gland-of-broilers
#6
Yongfen Wang, Zhen Zhang, Hongwei Guo, Pengying Li, Jingang Li, Lei Xi, Qiong Liu
Melatonin is a crucial neurohormone synthesized in the pineal gland that influences the physiology of animals. The molecular mechanism of norepinephrine control of the synthesis of melatonin is well documented; however, few reports have described the effects of epinephrine on the synthesis of melatonin. In this study, the effect of epinephrine on melatonin synthesis was investigated by adding different concentrations of epinephrine or norepinephrine to broiler pineal glands cultured in vitro. In addition, we investigated how epinephrine regulates the synthesis of melatonin and the transcription of the key melatonin synthesis enzyme AANAT...
September 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30178273/mesenchymal-stem-cells-attenuate-sepsis-induced-liver-injury-via-inhibiting-m1-polarization-of-kupffer-cells
#7
Xujing Liang, Taoyuan Li, Qiuchan Zhou, Sainan Pi, Yadan Li, Xiaojia Chen, Zeping Weng, Hongmei Li, Ying Zhao, Huadong Wang, Youpeng Chen
Sepsis is a leading cause of death in intensive care units that can result in acute hepatic damage. Animal experiments and clinical trials have shown that mesenchymal stem cell (MSC) therapy has some beneficial in several liver diseases. However, the protective effects of MSC therapy on sepsis-induced hepatic damage and associated mechanisms are not completely understood. The aim of the present study was to investigate the effects of MSCs on sepsis-induced liver injury and underlying mechanisms. A rat model of sepsis-induced liver injury was established by cecal ligation and puncture, and serum alanine aminotransferase and aspartate transaminase activities as well as liver histological changes were measured...
September 3, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30173373/potential-therapeutic-roles-of-10-dehydrogingerdione-and-or-pentoxifylline-against-calcium-deposition-in-aortic-tissues-of-high-dietary-cholesterol-fed-rabbits
#8
Mohamed M Elseweidy, Hoda E Mohamed, Rania A Elrashidy, Hebatallah H Atteia, Gehad M Elnagar, Abd El-Monem Ali
The present study aimed to investigate the inhibitory effects of 10-dehydrogingerdione (10-DHGD) and pentoxifylline (PTX) either individually or in combined form on calcium deposition in high cholesterol diet (HCD)-fed rabbits as compared to atorvastatin (ATOR), and to clarify the underlying mechanisms. Three-months-old male New Zealand white rabbits received either normal chow or HCD for 12 weeks. The latter group was subdivided into five groups and concurrently treated either with vehicle (dyslipidemic control), ATOR, 10-DHGD, PTX or combined 10-DHGD and PTX...
September 1, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30173372/tdcpp-protects-cardiomyocytes-from-h-2-o-2-induced-injuries-via-activating-pi3k-akt-gsk3%C3%AE-signaling-pathway
#9
Weishan Zhang, Xin Hou, Mengjun Huang, Xixi Zeng, Xiju He, Yanhong Liao
Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) is a major type of organophosphorus flame retardants, and long-term exposure to TDCPP to normal cells or tissues under physiological conditions can induce toxic effects. But how TDCPP leads to the adverse effects is not yet clear, and the effect of TDCPP under pathological conditions such as reactive oxygen species assault is not well understood. The present study aimed to explore the potential effect of TDCPP against H2 O2 -induced oxidative stress in H9c2 cardiomyoblasts and rat neonatal cardiomyocytes...
September 1, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30167938/vitamin-d-improves-vascular-function-and-decreases-monoamine-oxidase-a-expression-in-experimental-diabetes
#10
Adrian Sturza, Adrian Văduva, Diana Uțu, Corina Rațiu, Norbert Pop, Oana Duicu, Călin Popoiu, Eugen Boia, Petru Matusz, Danina M Muntean, Sorin Olariu
The active form of vitamin D, 1,25-dihydroxycholecalciferol (1,25(OH)2 D3 ), was reported to improve vascular function in patients with diabetes, yet the underlying mechanisms remain to be fully elucidated. Monoamine oxidase (MAO), a mitochondrial enzyme, with two isoforms (A and B) that generates hydrogen peroxide (H2 O2 ) as by-product, has been recently reported to contribute to the pathogenesis of endothelial dysfunction in diabetes. The present study assessed the interaction between vitamin D and MAO in the vascular wall in the setting of type 1 experimental diabetes...
August 30, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30159798/inhibitions-of-anandamide-transport-and-faah-synthesis-decrease-apoptosis-and-oxidative-stress-through-inhibition-of-trpv1-channel-in-an-in-vitro-seizure-model
#11
Mustafa Nazıroğlu, Afife Nur Taner, Esra Balbay, Bilal Çiğ
The expression level of TRPV1 is high in hippocampus which is a main epileptic area in the brain. In addition to the actions of capsaicin (CAP) and reactive oxygen species (ROS), the TRPV1 channel is activated in neurons by endogenous cannabinoid, anandamide (AEA). In the current study, we investigated the role of inhibitors of TRPV1 (capsazepine, CPZ), AEA transport (AM404), and FAAH (URB597) on the modulation of Ca2+ entry, apoptosis, and oxidative stress in in vitro seizure-induced rat hippocampus and human glioblastoma (DBTRG) cell line...
August 29, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30159797/expression-of-the-intrarenal-angiotensin-receptor-and-the-role-of-renin-angiotensin-system-inhibitors-in-iga-nephropathy
#12
Zheng Zhang, Shi-Min Jiang, Ye-Ping Ma, Pei-Lin Dai, Yi-Ning Wang, Gu-Ming Zou, Hong-Mei Gao, Yue Yang, Wen-Ge Li
The critical role of the intrarenal renin-angiotensin system (RAS) in the development of kidney disease has been well demonstrated in animal and cell-culture experiments, but evidence from human kidney tissues is lacking. In this study, we screened 438 patients with IgA nephropathy (IgAN) and analyzed their clinical characteristics. Renal biopsy revealed the expression of angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), and MAS receptor (MASR) in the tissues of 260 patients not treated with RAS inhibitors, 32 patients treated with angiotensin-converting enzyme inhibitors (ACEIs), and 89 patients treated with angiotensin receptor blockers (ARBs)...
August 29, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30159796/%C3%AE-bisabolol-abrogates-isoproterenol-induced-myocardial-infarction-by-inhibiting-mitochondrial-dysfunction-and-intrinsic-pathway-of-apoptosis-in-rats
#13
Mohamed Fizur Nagoor Meeran, Farah Laham, Sheikh Azimullah, Saeed Tariq, Shreesh Ojha
Mitochondrial dysfunction plays crucial role in the pathologenesis of myocardial infarction (MI). The present study evaluated the protective effect of α-bisabolol against isoproterenol (ISO)-induced mitochondrial dysfunction and apoptosis in rats. Male albino Wistar rats were pre- and co-treated with intraperitoneal injection of α-bisabolol (25 mg/kg body weight) daily for 10 days. To induce experimental MI, ISO (85 mg/kg body weight) was injected subcutaneously to the rats at an interval of 24 h for 2 days (9th and 10th day)...
August 29, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30155659/endocardial-endothelial-cell-hypertrophy-takes-place-during-the-development-of-hereditary-cardiomyopathy
#14
Danielle Jacques, Ghassan Bkaily
Endocardial endothelial cells constitute a barrier between the circulating blood and ventricular cardiomyocytes. Although recently our group demonstrated the importance of this type of endothelial cells in excitation-secretion coupling, there is no information on whether this type of cells contributes to cardiac pathologies such as cardiac hypertrophy. Using the well-known model of human hypertrophy and heart failure, the UM-X7.1 hereditary cardiomyopathic hamster, our results showed that during the phase of necrosis and in the absence of cardiac hypertrophy, isolated endocardial endothelial cells underwent a significant increase in cell volume compared to cells isolated from age-matched normal hamsters...
August 28, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30145644/supplementation-of-scopoletin-improves-insulin-sensitivity-by-attenuating-the-derangements-of-insulin-signaling-through-ampk
#15
Kalaivanan Kalpana, Chandrasekaran Sathiya Priya, Nanda Dipti, Ramachandran Vidhya, Carani Venkatraman Anuradha
Scopoletin (SPL), a phenolic coumarin, is reported to regulate glucose metabolism. This study is initiated to substantiate the action of SPL on the regulation of insulin signaling in insulin resistant RIN5f cells and high fat, high fructose diet (HFFD)-fed rat model. Adult male Sprague Dawley rats were fed HFFD for 45 days to induce type 2 diabetes and then treated or untreated with SPL for the next 45 days. The levels of glucose, insulin, lipid profile, oxidative stress markers along with insulin signaling and AMPK protein expressions were examined at the end of 90 days...
August 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30145643/giv-girdin-promotes-cell-survival-during-endoplasmic-reticulum-stress
#16
Peter Nguyen, Rosanna Calderon, Yoanna Rodriguez-Ledezma, Kelly Araujo, Deepali Bhandari
Endoplasmic reticulum (ER) stress is a form of cellular stress that is experienced by cells both under normal physiological conditions such as in professional secretory cells and disease states such as cancer, diabetes, and neurodegeneration. Upon facing ER stress, cells activate a conserved signaling pathway called the unfolded protein response (UPR) to restore normal function by halting general protein translation, upregulating expression of chaperones, and promoting ER-associated degradation. However, if the stress is overwhelming and cells are not able to recover within a reasonable time frame, the UPR ultimately commits cells to programmed cell death...
August 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30143989/wound-fluid-of-rats-fed-protein-free-diets-delays-wound-healing-through-the-suppression-of-the-igf-1-erk-1-2-signaling-pathway
#17
Takumi Yamane, Mari Shimura, Ryosuke Konno, Ken Iwatsuki, Yuichi Oishi
Adequate nutrition is required to maintain healthy skin integrity, and malnourished patients with poor protein diet often experience delayed wound healing. Understanding the cellular mechanisms of protein malnutrition will justify the importance of optimal protein diets in health and disease defense. Therefore in the present study, we examined the effects of changes in wound fluid composition and its function caused by protein malnutrition on wound healing. Rats were fed a control (CO; 20% protein) diet or a protein-free (PF) diet for 2 weeks; we then created full-thickness wounds on the dorsolateral skin...
August 24, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30136190/hgf-promotes-htr-8-svneo-cell-migration-through-activation-of-mapk-pka-signaling-leading-to-up-regulation-of-wnt-ligands-and-integrins-that-target-%C3%AE-catenin
#18
Piyush Chaudhary, Sudha Saryu Malhotra, Gosipatala Sunil Babu, Ranbir Chander Sobti, Satish Kumar Gupta
Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as intrauterine growth restriction and preeclampsia. In the present study, relevance of WNT ligands and integrins associated with hepatocyte growth factor (HGF)-mediated migration of HTR-8/SVneo trophoblastic cells has been investigated. Treatment of HTR-8/SVneo cells with HGF led to a dose-dependent increase in their migration. RT-PCR studies revealed a significant increase in the transcripts of WNT4, WNT11, ITGA2, and ITGAV, which was further confirmed at protein level by Western blotting...
August 22, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30132214/chlamydia-trachomatis-plasmid-encoded-protein-pgp3-inhibits-apoptosis-via-the-pi3k-akt-mediated-mdm2-p53-axis
#19
Yan Zou, Wenbo Lei, Shengmei Su, Jichang Bu, Shunxin Zhu, Qiulin Huang, Zhongyu Li
Chlamydia trachomatis, the most common human pathogen that causes trachoma and sexually transmitted disease, has developed various strategies for inhibiting host cell apoptosis. Activation of the PI3K (phosphoinositide 3-kinase)/AKT-mediated MDM2 (murine double minute 2)-p53 pathway plays a prominent role in the apoptosis resistance arising from C. trachomatis infection. However, the precise upstream mechanisms by which C. trachomatis activates this pathway have not been adequately investigated. Here, we reveal that the secreted C...
August 21, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/30128948/estimating-hemodynamic-shear-stress-in-murine-peripheral-collateral-arteries-by-two-photon-line-scanning
#20
Manuel Lasch, Katharina Nekolla, Anna H Klemm, Judith-Irina Buchheim, Ulrich Pohl, Steffen Dietzel, Elisabeth Deindl
Changes in wall shear stress of blood vessels are assumed to be an important component of many physiological and pathophysiological processes. However, due to technical limitations experimental in vivo data are rarely available. Here, we investigated two-photon excitation fluorescence microscopy as an option to measure vessel diameter as well as blood flow velocities in a murine hindlimb model of arteriogenesis (collateral artery growth). Using line scanning at high frequencies, we measured the movement of blood cells along the vessel axis...
August 20, 2018: Molecular and Cellular Biochemistry
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