Read by QxMD icon Read

Molecular and Cellular Biochemistry

Chengmin Huang, Peng Zhang, Tingting Li, Jun Li, Tianjiao Liu, Anju Zuo, Jiying Chen, Yuan Guo
This study was aimed to explore the role of C1q/TNF-related protein 9 (CTRP9) on atherosclerotic lesion formation. A recombinant lentiviral vector carrying mouse CTRP9 (Lv-CTRP9) was injected intravenously into apolipoprotein E knockout (ApoE-/- ) mice given a high-fat diet (HFD). CTRP9 overexpression substantially attenuated atherosclerotic lesion size of mice. The accumulation of macrophages and smooth muscle cells (SMCs) was significantly decreased in atherosclerotic regions with CTRP9 overexpression by immunohistochemical analysis...
November 13, 2018: Molecular and Cellular Biochemistry
Naoshi Odagiri, Tsutomu Matsubara, Moe Higuchi, Sayuri Takada, Hayato Urushima, Misako Sato-Matsubara, Yuga Teranishi, Katsutoshi Yoshizato, Norifumi Kawada, Kazuo Ikeda
Senescent hepatic stellate cells (senescent HSCs) are found in patients with liver cirrhosis and have been thought to be involved in the development of hepatocellular carcinoma (HCC) in mice via the senescence-associated secretory proteins. However, in humans, which secretory proteins are involved and what regulate their expression remain unclear. In the current study, we characterized senescence-associated β-galactosidase-positive senescent human HSCs (hHSCs) induced by repetitive passaging. They exhibited enhanced expression of 14 genes for secretory protein and persistent phosphorylation of ERK1/2 protein but not JNK or p38 MAPK proteins...
November 13, 2018: Molecular and Cellular Biochemistry
Stuart R Green, Kenneth B Storey
Carbamoyl phosphate synthetase I (CPS1) represents an important regulatory enzyme of the urea cycle that mediates the ATP-driven reaction ligating ammonium, carbonate, and phosphate to form carbamoyl phosphate. The freeze-tolerant wood frog (Rana sylvatica or Lithobates sylvaticus) accumulates high concentrations of urea during bouts of freezing to detoxify any ammonia generated and to contribute as a cryoprotectant thereby helping to avoid freeze damage to cells. Purification of CPS1 to homogeneity from wood frog liver was performed in control and frozen wood frogs by a three-step chromatographic process...
November 12, 2018: Molecular and Cellular Biochemistry
Christian Morsczeck, Anja Reck, Torsten E Reichert
The osteogenic differentiation of dental follicle cells (DFCs) is inhibited by the onset of cellular senescence, but the cause for this is largely unknown. Recently it was shown that WNT5a, which is an inductor of the non-canonical WNT pathway, stimulates both cellular senescence and osteogenic differentiation of different cell types. In this study, we investigated the role of WNT5a for viability and osteogenic differentiation in human DFCs after the induction of cellular senescence. DFCs were cultivated until the induction of cellular senescence...
November 7, 2018: Molecular and Cellular Biochemistry
Biji Chatterjee, Krishna Ghosh, Lavanya Suresh, Santosh R Kanade
The protein arginine methyltransferase 5 (PRMT5) and its catalytic partner methylosome protein MEP50 (WDR77) catalyse the mono- and symmetric di-methylation of selective arginines in various histones and non-histone target proteins. It has emerged as a crucial epigenetic regulator in cell proliferation and differentiation; which also reported to be overexpressed in many forms of cancers in humans. In this study, we aimed to assess the modulations in the expression of this enzyme upon exposure to the well-studied natural compound from the spice turmeric, curcumin...
November 3, 2018: Molecular and Cellular Biochemistry
Naveen Kumar, Nisha Yadav, Nagarjuna Amarnath, Vijeta Sharma, Swapnil Shukla, Akriti Srivastava, Peeyush Prasad, Anil Kumar, Swati Garg, Shailja Singh, Seema Sehrawat, Bimlesh Lochab
Natural products from medicinal plants have always attracted a lot of attention due to their diverse and interesting therapeutic properties. We have employed the principles of green chemistry involving isomerization, coupling and condensation reaction to synthesize a class of compounds derived from eugenol, a naturally occurring bioactive phytophenol. The compounds were characterized structurally by 1 H-, 13 C-NMR, FT-IR spectroscopy and mass spectrometry analysis. The purity of compounds was detected by HPLC...
November 3, 2018: Molecular and Cellular Biochemistry
Stelios Zerikiotis, Charalampos Angelidis, Irida Dhima, Katerina K Naka, Panagiota Kasioumi, Vasiliki Kalfakakou, Dimitrios Peschos, Patra Vezyraki
The aim of this study was to examine the potential association between the expression of Hsp70 protein and heart failure and to investigate the possible protective effect of Hsp70 against the doxorubicin-induced toxicity. Initially, at clinical level, the expression levels of the inducible Hsp70 were quantified in serum from patients with heart failure. Our results showed that in heart failure, Hsp70 concentration appeared to be increased in blood sera of patients compared to that of healthy individuals. The enhanced expression of Hsp70 in serum of patients with heart failure seemed to be associated with various features, such as gender, age and the type of heart failure, but not with its etiology...
November 2, 2018: Molecular and Cellular Biochemistry
Jianhong Wang, Xiangxiang Liu, Caixia Hao, Yingjuan Lu, Xiaohui Duan, Rong Liang, Guangxun Gao, Tao Zhang
T cells are involved in bone marrow failure in aplastic anemia (AA). MEG3 is a long, non-coding RNA that can modulate target gene expression and T cell differentiation by acting as a microRNA sponge. Our previous study showed that T cell immunoglobulin and immunoreceptor tyrosine-based inhibition motif (ITIM) domain (TIGIT) plays a critical role in regulating CD4 + T cell functions. In this study, we found that MEG3 expression was significantly downregulated in CD4 + T cells derived from AA patients...
October 31, 2018: Molecular and Cellular Biochemistry
Dina Sabry, Sahar E M El-Deek, Moataz Maher, Mona A H El-Baz, Hala M El-Bader, Eman Amer, Elham A Hassan, Wael Fathy, Heba E M El-Deek
Colorectal cancer (CRC) is a major cause of death worldwide. Novel non-invasive, high diagnostic value screening test is urgently needed to improve survival rate, treatment and prognosis. Stable, small, circulating microRNA (miRNA) offers unique opportunities for the early diagnosis of several diseases. It acts as tumor oncogenes or suppressors and involve in cell death, survival, and metastasis. Communication between miRNA and carcinogenesis is critical but it still not clear and needs further investigation...
October 24, 2018: Molecular and Cellular Biochemistry
George H Kunkel, Christopher J Kunkel, Hazel Ozuna, Irina Miralda, Suresh C Tyagi
Heart failure (HF) is a functional lack of myocardial performance due to a loss of molecular control over increases in calcium and ROS, resulting in proteolytic degradative advances and cardiac remodeling. Mitochondria are the molecular powerhouse of cells, shifting the sphere of cardiomyocyte stability and performance. Functional mitochondria rely on the molecular abilities of safety factors such as TFAM to maintain physiological parameters. Mitochondrial transcription factor A (TFAM) creates a mitochondrial nucleoid structure around mtDNA, protecting it from mutation, inhibiting NFAT (ROS activator/hypertrophic stimulator), and transcriptionally activates Serca2a to decrease calcium mishandling...
October 23, 2018: Molecular and Cellular Biochemistry
Toyoki Maeda, Takahiko Horiuchi, Naoki Makino
Somatic telomere DNA length is known to shorten with certain disease states and senescence. Furthermore, we have reported that the telomere length of a sub-healthy population also correlates with the blood data of laboratory tests. These facts suggest that patients with shorter telomere length tend to be hospitalized more easily than patients with longer telomere length. And such hospitalization tendencies can also be reflected in differences in clinical laboratory data. To address this issue, we evaluated and compared the telomere length and clinical laboratory data of outpatients and inpatients...
October 23, 2018: Molecular and Cellular Biochemistry
Louis T P Martin, Mark W Nachtigal, Tamara Selman, Elaine Nguyen, Jayme Salsman, Graham Dellaire, Denis J Dupré
Bitter taste receptors (Tas2Rs) are a subfamily of G-protein coupled receptors expressed not only in the oral cavity but also in several extra-oral tissues and disease states. Several natural bitter compounds from plants, such as bitter melon extract and noscapine, have displayed anti-cancer effects against various cancer types. In this study, we examined the prevalence of Tas2R subtype expression in several epithelial ovarian or prostate cancer cell lines, and the functionality of Tas2R14 was determined. qPCR analysis of five TAS2Rs demonstrated that mRNA expression often varies greatly in cancer cells in comparison to normal tissue...
October 22, 2018: Molecular and Cellular Biochemistry
Fang Liu, YuHua Zhao, JieMin Lu, ShuangHui Chen, XinGuang Zhang, WenWei Mao
Mycoplasma pneumoniae pneumonia (MPP) is the most common respiratory infection in young children and its incidence has increased worldwide. In this study, high expression of chemokine ligand 5 (CCL5) was observed in the serum of MPP patients, and its expression was positively correlated to DNA of M. pneumoniae (MP-DNA). In vitro, M. pneumoniae (MP) infection to A549 cells induced the expression of CCL5, chemokines receptor 4 (CCR4), nuclear factor-κB (NF-κB) nuclear protein, and phosphorylation of NF-κB-p65 (p-NF-κB-p65), whereas NF-κB cytoplasmic protein was decreased...
October 22, 2018: Molecular and Cellular Biochemistry
Sanju Kumari, Abhishek Mukherjee, Chinmay K Mukhopadhyay
Recent literature suggested an important function of native amyloid precursor protein (APP) as amine oxidase implicating in protection of brain cells from catecholamine-induced toxicity. However, any role of catecholamines on regulation of native APP has not been explored. Here we report that dopamine (DA), one of the most prominent catecholamine neurotransmitters in brain, down-modulates native APP protein in several neuronal cell types. Using SH-SY5Y cells as model, we detected no alteration of transcript expression and unaffected translation suggested that DA might induce APP degradation...
October 22, 2018: Molecular and Cellular Biochemistry
Jingxuan Fu, Hui Zhang, Yuling Zhang, Tao Zhang
Neuroblastoma is the most common extra-cranial tumor in childhood. As an antineoplastic medicine, the effect of AG-1031 on the neuroblastoma is still unclear. Silent information regulator 1 (SIRT1) is a conserved NAD+ -dependent deacetylase, which plays a key role in carcinogenesis through the deacetylation of important regulatory proteins, including p53. The purpose of the present study was to determine whether there was a significant anti-tumor effect of AG-1031 on the human neuroblastoma cells through suppressing SIRT1/p53 pathway...
October 22, 2018: Molecular and Cellular Biochemistry
Peipei Wang, Jinming Wu, Zhonghong Gao, Hailing Li
Serum albumin (SA) has been shown to act as a heme scavenger in hemolysis and can protect cell against the toxic effect of heme. However, the mechanism of SA in heme detoxification is not well understood. Interestingly, increasing studies indicate that heme/H2 O2 -dependent reaction is unlikely to be the principal cause of heme toxicity in excessive intravascular hemolysis conditions. Moreover, high levels of NO2 - and NO3 - were also found in patients with severe hemolytic diseases, which seem to involve in heme toxic effect as well...
October 15, 2018: Molecular and Cellular Biochemistry
Biljana Jakovljevic, Tamara Nikolic Turnic, Nevena Jeremic, Jovana Jeremic, Jovana Bradic, Marko Ravic, Vladimir Lj Jakovljevic, Djordje Jelic, Dragan Radovanovic, Olga Pechanova, Vladimir Zivkovic
This study was aimed to assess the impact of aerobic and anaerobic type of exercise on blood pressure and redox status in normotensive and hypertensive rats. After 1 week of preconditioning feeding and 1 week of preconditioning running regimen, Wistar albino rats (n = 72; bw: 270 ± 50 g) were randomly assigned to three groups according to running protocol (high-intensity interval training (HIIT) or moderate-intensity training (MIT)): sedentary control, MIT, HIIT; spontaneous hypertensive sedentary control (SHR), SHR + MIT and SHR + HIIT...
October 11, 2018: Molecular and Cellular Biochemistry
Xin Li, Ming Dong, Jianping Zhou, Dehua Zhu, Jinbo Zhao, Weiwei Sheng
C6orf106 was highly expressed in lung and breast cancer, and proposed as clinicopathologic factor for the development of those types of cancer. However, its expression in pancreatic cancer and the mechanism that C6orf106 functions as an oncogene has not been confirmed. In the present study, we found that C6orf106 was also up-regulated in pancreatic cancer tissues and cell lines. Furthermore, C6orf106 expression was associated with advanced T stage (P = 0.010), positive regional lymph node metastasis (P = 0...
October 11, 2018: Molecular and Cellular Biochemistry
Finosh G Thankam, Chandra S Boosani, Matthew F Dilisio, R Michael Gross, Devendra K Agrawal
Fatty infiltration and inflammation delay the healing responses and raise major concerns in the therapeutic management of rotator cuff tendon injuries (RCTI). Our evaluations showed the upregulation of 'metabolic check point' AMPK and inflammatory molecule, TREM-1 from shoulder biceps tendons collected from RCTI subjects. However, the epigenetic regulation of these biomolecules by miRNAs is largely unknown and it is likely that a deeper understanding of the mechanism of action can have therapeutic potential for RCTI...
October 10, 2018: Molecular and Cellular Biochemistry
Xueming Tan, Xiaolu He, Zhining Fan
3-Hydroxy-3-methylglutaryl reductase degradation (HRD1) is an E3 ubiquitin ligase that functions by promoting degradation of misfolded proteins in processes such as embryogenesis and rheumatoid arthritis. However, little is known about the role of HRD1 in cancer. The aim of the present study was to investigate the expression pattern and functions of HRD1 in human colon cancer (CC). We found that HRD1 expression was increased significantly in human CC tissues, and its overexpression was associated with TNM stage, tumor differentiation, tumor invasive depth, and distant metastasis...
October 10, 2018: Molecular and Cellular Biochemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"