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Annals of Clinical Biochemistry

John W Honour
No abstract text is available yet for this article.
November 2017: Annals of Clinical Biochemistry
Mohamed Saleem, Helen Martin, Anne Tolya, Penny Coates
Background Interference from opiates in the Microgenics CEDIA® Buprenorphine assay is known to produce false-positive buprenorphine screening immunoassay results necessitating confirmatory buprenorphine testing by chromatography/mass spectrometry methods. Method We reviewed data on falsely positive buprenorphine immunoassay screen (cut-off ≥ 5  µg/L) but negative for buprenorphine by gas chromatography mass spectrometry (cut-off ≥ 5  µg/L) and had a positive opiate immunoassay result (cut-off ≥ 300  µg/L)...
November 2017: Annals of Clinical Biochemistry
Takashi Yamaguchi, Takeyoshi Murano, Ichiro Tatsuno, Nobuyuki Hiruta, Toru Suzuki, Shojiro Sawada, Hideki Katagiri, Kohji Shirai, Wolfgang J Schneider, Hideaki Bujo
Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsified with Triton X-100. Methods Lipolytic activities of plasma obtained by heparin injection from a homozygous patient with LPL Arg243His were analysed using triolein emulsified with phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), or Triton X-100 as substrates...
November 2017: Annals of Clinical Biochemistry
Eriko Mori, Makoto Kurano, Akiko Tobita, Hironori Shimosaka, Yutaka Yatomi
Background Squamous cell carcinoma antigen is used as a tumour marker and is routinely measured in clinical laboratories. We validated two different immunoassays and found three cases in which the squamous cell carcinoma antigen concentrations deviated greatly between the two immunoassays. Here, we aimed to elucidate the mechanisms responsible for these deviations. Methods The squamous cell carcinoma antigen concentrations were determined using the ARCHITECT SCC (CLIA method) and the ST AIA-PACK SCC (FEIA method)...
November 2017: Annals of Clinical Biochemistry
Mary Stapleton, Niamh Daly, Ruth O'Kelly, Michael J Turner
Background The inhibition of glycolysis prior to glucose measurement is an important consideration when interpreting glucose tolerance tests. This is particularly important in gestational diabetes mellitus where prompt diagnosis and treatment is essential. A study was planned to investigate the effect of preservatives and temperature on glycolysis. Methods Blood samples for glucose were obtained from consented females. Lithium heparin and fluoride-EDTA samples transported rapidly in ice slurry to the laboratory were analysed for glucose concentration and then held either in ice slurry or at room temperature for varying time intervals...
November 2017: Annals of Clinical Biochemistry
Pete Ayling, Robert Hill, Nuthar Jassam, Anders Kallner, Zahra Khatami
Background A logical consequence of the introduction of robotics and high-capacity analysers has seen a consolidation to larger units. This requires new structures and quality systems to ensure that laboratories deliver consistent and comparable results. Methods A spreadsheet program was designed to accommodate results from up to 12 different instruments/laboratories and present IQC data, i.e. Levey-Jennings and Youden plots and comprehensive numerical tables of the performance of each item. Input of data was made possible by a 'data loader' by which IQC data from the individual instruments could be transferred to the spreadsheet program on line...
November 2017: Annals of Clinical Biochemistry
Michal Ws Ong, Rashim Salota, Tracy Reeman, Marta Lapsley, Lydia Jones
The most commonly used techniques to measure vitamin D are automated immunoassays which are known to be affected by interferences, especially from immunoglobulins present in the patient's serum. We present a case of a patient with myeloma in whom interference with the vitamin D assay was identified. An 83-year-old female, known to have IgG myeloma, was found to have a high concentration of 25-OH vitamin D on a routine test without any signs of vitamin D toxicity. She was not taking vitamin D supplements or any other multivitamin preparation and had minimal sun exposure...
November 2017: Annals of Clinical Biochemistry
John M Wadsworth, Anna M Milan, James Anson, Andrew S Davison
Background Azole-based antifungals are the first-line therapy for some of the most common mycoses and are now also being used prophylactically to protect immunocompromised patients. However, due to variability in both their metabolism and bioavailability, therapeutic drug monitoring is essential to avoid toxicity but still gain maximum efficacy. Methods Following protein precipitation of serum with acetonitrile, 20  µL of extract was injected onto a 2.1 × 50 mm Waters Atlantis dC18 3  µm column...
November 2017: Annals of Clinical Biochemistry
Yuan-Lan Huang, Zhi-Jun Han, Zhi-De Hu
Background Red blood cell distribution width (RDW) and neutrophil to lymphocyte ratio (NLR) have been reported to be associated with outcomes of acute cerebral infarction. However, their prognostic value in patients with subarachnoid haemorrhage (SAH) remains largely unknown. The aim of this study was to investigate the prognostic value of RDW and NLR in SAH patients. Methods Medical records of adult SAH patients admitted to intensive care unit (ICU) were extracted from Multiparameter Intelligent Monitoring in Intensive Care II (MIMIC II, version 2...
November 2017: Annals of Clinical Biochemistry
Yumiko Akamine, Yuka Sugawara-Kikuchi, Tsukasa Uno, Tetsuo Shimizu, Masatomo Miura
Background This study developed a novel high-performance liquid chromatography (HPLC) method for the simultaneous quantification of clozapine and its active metabolite, N-desmethylclozapine, in human plasma and investigated the effects of various factors, including genetic polymorphisms in cytochrome P450 (CYP) 2D6, CYP3A5, ABCB1 and ABCG2, on the steady-state plasma trough concentrations (C0) of clozapine and N-desmethylclozapine in Japanese patients with schizophrenia. Methods Forty-five patients had been receiving fixed doses of clozapine for at least four weeks...
November 2017: Annals of Clinical Biochemistry
Yukari Kajiura, Jung-Hwan Lew, Takahisa Ikuta, Yasufumi Nishikawa, Jun-Ichi Kido, Toshihiko Nagata, Koji Naruishi
Background Periodontitis is an inflammatory disease. The aim of this study was to investigate whether the soluble form of interleukin-6 receptor (sIL-6R) and calprotectin concentrations in gingival crevicular fluid are useful biomarkers in the evaluation of periodontitis. Methods First, a cross-sectional study was performed. A total of 34 periodontitis patients were enrolled and the gingival crevicular fluid samples were collected from the healthy and inflamed sites of periodontal pockets in each patient. The relationship between periodontal condition and gingival crevicular fluid sIL-6R and calprotectin concentrations was analysed statistically...
November 2017: Annals of Clinical Biochemistry
Andrew Duncan, Calum Yacoubian, Robert Beetham, Anthony Catchpole, David Bullock
Background US and European guidelines suggest the use of calculated non-caeruloplasmin-bound copper (free copper index) for the diagnosis and management of Wilson's Disease. However, there is concern that the required analytical measurements of caeruloplasmin and copper may not be sufficiently robust at the concentrations usually found. Methods Aliquots of six plasma specimens were sent to laboratories participating in the UK National External Quality Assessment Scheme for copper and caeruloplasmin. The variability of these two reported measurements and the calculated non-caeruloplasmin-bound copper concentrations were compared...
November 2017: Annals of Clinical Biochemistry
Seyyed Mr Kazemi-Bajestani, Maryam Tayefi, Mahmoud Ebrahimi, Ali R Heidari-Bakavoli, Mohsen Moohebati, Seyyed Mr Parizadeh, Habibollah Esmaeili, Gordon Aa Ferns, Majid Ghayour-Mobarhan
Background Metabolic syndrome is defined by a clustering of cardiovascular risk factors and is associated with a heightened inflammatory state. A raised serum high-sensitivity C-reactive protein, a marker of inflammation, is also known to associate with cardiovascular risk. We have investigated the relationship between the presence of metabolic syndrome and serum high-sensitivity C-reactive protein concentration in a large representative Persian population cohort without a history of cardiovascular disease...
November 2017: Annals of Clinical Biochemistry
Elif Azarsız, Neslihan Karaca, Erturk Levent, Necil Kutukculer, Eser Sozmen
Background Common variable immunodeficiency is a rare clinically symptomatic primary immunodeficiency disorder which manifests a wide variability of symptoms, complications. Atherosclerosis in common variable immunodeficiency patients has not been investigated yet contrary to other severe clinical complications. We aimed to investigate the chitotriosidase enzyme's role as an inflammation and atherosclerosis marker in paediatric common variable immunodeficiency patients. Methods Common variable immunodeficiency patients (n = 24) and healthy controls (n = 23) evaluated for chitotriosidase activity with other inflammation markers (hsCRP, myeloperoxidase, serum amyloid A, ferritin), lipid profile and echocardiographic findings (carotid artery intima media thickness - cIMT, brachial artery flow-mediated vazodilatation - FMD%)...
November 2017: Annals of Clinical Biochemistry
Shinya Inada, Masafumi Koga
Background The effect of alcohol consumption on glycaemic control indicators is not well known. In this study, we studied the effect of alcohol consumption on the plasma glucose and glycaemic control indicators in non-diabetic men. Methods The study enrolled 300 non-diabetic men who received a complete medical checkup (age: 52.8 ± 6.5 years, body mass index: 24.4 ± 2.8 kg/m(2)). The subjects were divided into four groups by the amount of alcohol consumed, and the plasma glucose, HbA1c, glycated albumin (GA) and 1,5-anhydroglucitol (1,5-AG) concentrations of the groups were compared...
November 2017: Annals of Clinical Biochemistry
Michael J Murphy
No abstract text is available yet for this article.
September 2017: Annals of Clinical Biochemistry
Stuart J Moat, Derek Rees, Roanna S George, Lawrence King, Alan Dodd, Adeboye Ifederu, Tejswurree Ramgoolam, Sharon Hillier
Background Tandem mass spectrometry (MS/MS) has recently become an alternative method for the newborn screening of sickle cell disorders (SCD), as it is able to detect haemoglobin (Hb) peptides following digestion of bloodspots with trypsin. Using the SpOtOn Diagnostics Reagent Kit, we previously developed a screening protocol to detect only the disease states of SCD, using action values based on the ratio between the variant Hb peptide to wild-type peptide abundances for the HbS, C, D(Punjab), O(Arab), E and Lepore peptides...
September 2017: Annals of Clinical Biochemistry
Ishwarlal Jialal
No abstract text is available yet for this article.
September 2017: Annals of Clinical Biochemistry
Lauren E Hughes, Clare Ford, Anjala Ekbote, Rousseau Gama
No abstract text is available yet for this article.
September 2017: Annals of Clinical Biochemistry
Melanie Davies, Grammati Sarri, Mary Ann Lumsden
No abstract text is available yet for this article.
September 2017: Annals of Clinical Biochemistry
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