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Annals of Clinical Biochemistry

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https://www.readbyqxmd.com/read/29433326/comment-on-standardization-of-steroid-tests-and-implications-for-the-endocrine-community
#1
David Ducroq, Annette Thomas
No abstract text is available yet for this article.
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29433325/comment-on-standardization-of-steroid-tests-and-implications-for-the-endocrine-community
#2
Jonathan G Middle
No abstract text is available yet for this article.
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29433324/letter-in-response-to-john-w-honour-standardization-of-steroid-tests-and-implications-for-the-endocrine-community-annals-of-clinical-biochemistry-vol-54-6-618-630
#3
https://www.readbyqxmd.com/read/29433323/standardization-of-steroid-tests-and-implications-for-the-endocrine-community-author-s-reply
#4
John W Honour
No abstract text is available yet for this article.
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29430934/liquid-chromatography-tandem-mass-spectrometry-challenges-in-introducing-published-methods-into-the-clinical-laboratory
#5
Milad Khedr, Anna M Milan, Andrew S Davison
No abstract text is available yet for this article.
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29430933/a-letter-in-response-to-liquid-chromatography-tandem-mass-spectrometry-challenges-in-introducing-published-methods-into-the-clinical-laboratory-by-khedr-et-al
#6
David J Marshall, Joanne E Adaway, Brian G Keevil
No abstract text is available yet for this article.
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29368538/the-bone-remodelling-cycle
#7
J S Kenkre, Jhd Bassett
The bone remodelling cycle replaces old and damaged bone and is a highly regulated, lifelong process essential for preserving bone integrity and maintaining mineral homeostasis. During the bone remodelling cycle, osteoclastic resorption is tightly coupled to osteoblastic bone formation. The remodelling cycle occurs within the basic multicellular unit and comprises five co-ordinated steps; activation, resorption, reversal, formation and termination. These steps occur simultaneously but asynchronously at multiple different locations within the skeleton...
May 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29169259/assessing-biomarker-status-of-vitamin-b-12-in-the-laboratory-no-simple-solution
#8
Catherine F Hughes, Helene McNulty
No abstract text is available yet for this article.
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29169257/evaluation-of-the-analytical-and-clinical-performance-of-the-fujirebio-lumipulse%C3%A2-g-25-oh-vitamin-d-assay
#9
Silvia Giuliani, Daisy Corvetta, Manuela Lucchiari, Markus Herrmann
No abstract text is available yet for this article.
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28875734/characterization-of-the-scope-and-magnitude-of-biotin-interference-in-susceptible-roche-elecsys-competitive-and-sandwich-immunoassays
#10
Christina Trambas, Zhong Lu, Tina Yen, Ken Sikaris
Background Biotin interference is a significant problem to which at-risk laboratories must now be attuned. We sought to systematically characterize the nature of this interference in Roche immunoassays. Methods Known concentrations of biotin were titrated into serum samples and the effects on competitive and sandwich immunoassays were analysed. The maximum and minimum concentrations examined reflect those likely to be achieved in individuals on 5 to 10 mg supplements at the lower end, and 100 to 300 mg biotin at the high end...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28661202/demand-management-by-electronic-gatekeeping-of-test-requests-does-not-influence-requesting-behaviour-or-save-costs-dramatically
#11
Aarti K Pema, Olivia Kiabilua, Tahir S Pillay
Background Healthcare budgets face constraints, and laboratories have developed strategies to adapt to the concomitant increase in workload. Some of the tests (7.4%) may be attributed to unnecessary repeat testing. Electronic gatekeeping has been implemented at selected laboratories in South Africa to limit unnecessary repeat testing. We performed a study of chemistry tests subjected to electronic gatekeeping to determine its effectiveness as a sustainable demand management tool. Methods A 22-month retrospective study of chemistry test requests at a Pretoria hospital was performed...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28661201/diagnostic-accuracy-of-the-coopscore-%C3%A2-to-predict-liver-fibrosis-in-human-immunodeficiency-virus-hepatitis-b-virus-co-infection
#12
Ludmia Taibi, Anders Boyd, Nelly Bosselut, Julie Bottero, Jérôme Guéchot, Karine Lacombe, Elisabeth Lasnier, Bruno Baudin, Michel Vaubourdolle
Background Non-invasive methods for assessing liver fibrosis are increasingly used as an alternative to liver biopsy. Recently, a score-based biochemical blood test (Coopscore© ) was developed in a cohort of patients chronically infected with hepatitis C virus, showing higher diagnostic performances than Fibrometer®, Fibrotest®, Hepascore® and Fibroscan™. Here, we assess its performance in patients co-infected with the human immunodeficiency virus and hepatitis B virus. Methods Ninety-seven human immunodeficiency virus/hepatitis B virus co-infected patients with liver biopsies were included from a previously described cohort...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28661200/decreased-serum-glicentin-concentration-in-patients-with-severe-and-morbid-obesity
#13
Juliette Raffort, Patricia Panaïa-Ferrari, Fabien Lareyre, Mathilde Blois, Pascale Bayer, Pascal Staccini, Patrick Fénichel, Giulia Chinetti
Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in individuals with adult obesity and to study its potential link with various metabolic parameters. Methods Fifty-two individuals with normal body mass index (BMI < 25 kg/m2 ) and 39 patients with severe or morbid obesity (BMI > 35 kg/m2 ) were prospectively included at the University Hospital of Nice between January 2014 and April 2016...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28656819/urinary-albumin-a-risk-marker-under-requested-in-primary-care-in-spain
#14
Maria Salinas, Maite López-Garrigós, Emilio Flores, Carlos Leiva-Salinas
Background To study the inter-practice regional variation in the request of urinary albumin by general practitioners and compare to guideline recommendations. Methods A cross-sectional study enrolled laboratories from different Spanish autonomous communities. Laboratories were invited to report the number of urinary albumin requested by general practitioners during 2012 and 2014. Urinary albumin requested per 1000 inhabitants and the index of variability (90th centile/10th centile) was calculated, and compared between autonomous communities and time periods...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28656817/high-sensitivity-troponin-t-and-i-reflect-left-atrial-function-being-assessed-by-cardiac-magnetic-resonance-imaging
#15
Michèle Natale, Michael Behnes, Seung-Hyun Kim, Julia Hoffmann, Nadine Reckord, Ursula Hoffmann, Johannes Budjan, Siegfried Lang, Martin Borggrefe, Theano Papavassiliu, Thomas Bertsch, Ibrahim Akin
Background Left atrial function (LAF) plays an interactive role between pulmonary and systemic circulation. Cardiac biomarkers, such as amino-terminal pro-brain natriuretic peptide (NT-proBNP) and troponins, might reflect cardiac function. This study aims to evaluate the association between high sensitivity troponins (hsTn) and left atrial function in patients undergoing cardiac magnetic resonance imaging (cMRI). Methods Patients undergoing cardiac magnetic resonance imaging (cMRI) were enrolled prospectively...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28656816/quantitative-measurement-of-a-candidate-serum-biomarker-peptide-derived-from-%C3%AE-2-hs-glycoprotein-and-a-preliminary-trial-of-multidimensional-peptide-analysis-in-females-with-pregnancy-induced-hypertension
#16
Kensuke Hamamura, Mitsuaki Yanagida, Hitoshi Ishikawa, Michio Banzai, Hiroshi Yoshitake, Daisuke Nonaka, Kenji Tanaka, Mayumi Sakuraba, Yasuka Miyakuni, Kenji Takamori, Michio Nojima, Koyo Yoshida, Hiroshi Fujiwara, Satoru Takeda, Yoshihiko Araki
Purpose We previously attempted to develop quantitative enzyme-linked immunosorbent assay (ELISA) systems for the PDA039/044/071 peptides, potential serum disease biomarkers (DBMs) of pregnancy-induced hypertension (PIH), primarily identified by a peptidomic approach (BLOTCHIP®-mass spectrometry (MS)). However, our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein341-367 ), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using liquid chromatography-multiple reaction monitoring (LC-MRM)/MS, to explore the potential utility of PDA071 as a DBM for PIH...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28656812/effects-of-bilirubin-photoisomers-on-the-measurement-of-direct-bilirubin-by-the-bilirubin-oxidase-method
#17
Hitoshi Okada, Kou Kawada, Susumu Itoh, Miyo Ozaki, Isami Kakutani, Takeshi Arai, Kosuke Koyano, Saneyuki Yasuda, Takashi Iwase, Koji Murao, Takashi Kusaka
Background We occasionally encounter increases in direct bilirubin value on reanalysis of the surplus serum collected in the past from a neonate with indirect hyperbilirubinemia. But the details of this phenomenon are unclear. We evaluated the change of direct bilirubin and the relation of bilirubin photoisomer of the serum exposed to room light. Methods Surplus serum samples from neonates with indirect hyperbilirubinemia were exposed to room light for 24 h. The bilirubin fraction assay of samples was performed by the bilirubin oxidase method (Nescauto and Aqua-auto Kainos reagent) and high-performance liquid chromatography...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28490184/electrophoretic-patterns-post-daratumumab
#18
Joanna Sheldon, Rachel D Wheeler, Ray Powles
Background Daratumumab (Darzalex) is a human IgG1 kappa monoclonal antibody targeting CD38 that has been recently approved for the treatment of refractory multiple myeloma. As it is a monoclonal protein, it can be detected on routine serum protein electrophoresis and by immunofixation. Methods Serum samples from four patients were analysed by serum protein electrophoresis immediately pre- and post-treatment with daratumumab. Results For all four patients, daratumumab was visible on serum protein electrophoresis as an additional small band (approximately 1 g/L) in the slow gamma region...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28490181/designing-and-evaluating-autoverification-rules-for-thyroid-function-profiles-and-sex-hormone-tests
#19
Jiancheng Li, Bizhen Cheng, Huizhen Ouyang, Tongtong Xiao, Jing Hu, Yingmu Cai
Purpose Following the analytical phase, the current practice of many hospital laboratories involves the manual verification of all test results followed by the production of the report. However, manual verification is a time-consuming and tedious process. In this paper, we provide a detailed description of how to design autoverification rules for thyroid function test profiles and sex hormones. Materials and methods We used DM2 (Data manager 2) to construct the algorithm and build the database for autoverification of thyroid function test profiles and sex hormones, with reference to Boolean logic, Auto 10-A and CLSI'88...
March 2018: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28485173/reactivity-of-bilirubin-photoisomers-on-the-measurement-of-direct-bilirubin-using-vanadic-acid-method
#20
Hitoshi Okada, Susumu Itoh, Shohei Kawamoto, Miyo Ozaki, Takashi Kusaka
Objective Investigation of the reactivity of fractions of bilirubin photoisomers with the vanadic acid oxidation method. Methods Bilirubin photoisomers were prepared by irradiating a bilirubin/human serum albumin solution with blue light emitting diode. Direct bilirubin and bilirubin fractions were measured using the vanadic acid oxidation method and high-performance liquid chromatography in the sample before and after irradiation. Results Direct bilirubin was increased in the solution containing bilirubin photoisomers...
March 2018: Annals of Clinical Biochemistry
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