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Annals of Clinical Biochemistry

Aniko Rentka, Krisztina Koroskenyi, Jolan Harsfalvi, Zoltan Szekanecz, Gabriella Szucs, Peter Szodoray, Adam Kemeny-Beke
The human precorneal tear film (PCTF) is a special body fluid, since it is a complex mixture of proteins, lipids small bioactive molecules and their concentrations and relative distribution represent not only the metabolic state of the ocular surface but also the systemic and local homeostasis of the outer eye and the human body. This suggests that biochemical analysis of the PCTF composition may provide a non-invasive tool for diagnosis and monitoring of disease progression or treatment efficacy in human medicine...
January 1, 2017: Annals of Clinical Biochemistry
Marijana Miler, Nora Nikolac
Background Microscopic examination of samples with negative dipstick results is not necessary. The aims of this study were to: (i) assess the risk of excluding urine sediment examination with negative dipstick results and (ii) calculate time savings by introducing this process. Methods The risk analysis was done for samples with negative urine dipstick and positive sediment findings. Possible missed elements in sediment were defined as 21 errors. Time saving was calculated as average time for preparation and examination sediments...
January 1, 2017: Annals of Clinical Biochemistry
Xi Zhao, Hui-Wen Zhang, Sha Li, Yan Zhang, Rui-Xia Xu, Cheng-Gang Zhu, Na-Qiong Wu, Yuan-Lin Guo, Ping Qing, Xiao-Lin Li, Geng Liu, Qian Dong, Jing Sun, Jianjun Li
BACKGROUND: Plasma proprotein convertase subtilisin/kexin type 9 (PCSK9) has been reported to be related to several risk factors and diseases such as inflammatory markers and coronary artery disease (CAD). The aim of present study was to investigate whether plasma PCSK9 level was associated with coronary artery calcification (CAC). METHODS: A total of 403 consecutive untreated patients with angina-like chest pain who received electron beam computed tomography (EBCT) were enrolled and a CAC score (CACS) was also measured...
January 1, 2017: Annals of Clinical Biochemistry
Karolina Maria Stepien, Mark Guy
Background The enzymatic method of caeruloplasmin measurement is based on copper-dependent oxidase activity. The advantage of the oxidase determination is that it has a much lower detection limit compared to immunoassay-based methods. It has found its application in both the diagnosis of Wilson's disease and also in the monitoring of patients' response to treatment. METHODS: The method previously described by Schosinsky(1)(2) was adapted for use on a 96-well plate. Caeruloplasmin oxidase activity results were derived from the equation: caeruloplasmin oxidase activity= (A15-A5) x 185 U/L...
January 1, 2017: Annals of Clinical Biochemistry
Mary Peavey, Neval Akbas, William Gibbons, Paul Zarutskie, Sridevi Devaraj
BACKGROUND: Measurement of estradiol is an integral component for management of ovarian stimulation for in vitro fertilization (IVF). Automated immunoassays offer fast assay times and high throughput, with less sensitivity and specificity. The aim of this study is to optimize the estradiol assay in patients undergoing ovarian stimulation for IVF via comparison of estradiol values obtained using two immunoassays compared to mass spectrometry. METHODS: Patients undergoing ovarian stimulation were prospectively recruited...
January 1, 2017: Annals of Clinical Biochemistry
Itir Yegenaga, Fatih Kamis, Canan Baydemir, Elizade Erdem, Koray Celebi, Necmi Eren, Nur Baykara
AIMS: The prevention of acute kidney injury (AKI) can be lifesaving for intensive care unit (ICU) patients. However, conventional methods are not sufficient for predicting the risk for approaching AKI. In this study, the promising biomarker NGAL(neutrophil gelatinase-associated lipocalin) was compared to CysC (Cystatin C ) for indicating risk of future AKI. METHODS: One hundred eighty-three adult patients without chronic kidney disease or renal replacement therapy were included in this study...
January 1, 2017: Annals of Clinical Biochemistry
Luis García de Guadiana Romualdo, María Dolores Albaladejo Otón, Sergio Rebollo Acebes, Patricia Esteban Torrella, Ana Hernando-Holgado, Enrique Jiménez Santos, Roberto Jiménez Sánchez, Alejandro Ortín Freire
BACKGROUND: Biomarkers can facilitate the diagnosis of sepsis, enabling early management and improving outcomes. Lipopolysaccharide binding protein (LBP) has been reported as a biomarker for detection of infection, but its diagnostic value is controversial. In this study, we assessed the diagnostic accuracy of LBP for sepsis in emergency department (ED) patients, comparing it with more established biomarkers of sepsis, including procalcitonin (PCT) and C-reactive protein (CRP). METHODS: LBP and other sepsis biomarkers, including PCT and CRP, were measured on admission in 102 adult patients presenting with suspected infection...
January 1, 2017: Annals of Clinical Biochemistry
Masakazu Nakamura, Tatsuyuki Fukukawa, Kazuo Kitagawa, Yoji Nagai, Naohisa Hosomi, Masayasu Matsumoto, Kazuo Minematsu, Shinichiro Uchiyama, Yoshihiro Miyamoto
BACKGROUND: The Japan Statin Treatment Against Recurrent Stroke (J-STARS) is a clinical trial that administered pravastatin for secondary stroke prevention. Lipid levels are a well- established risk factor for cerebrovascular diseases. Elevated high-sensitivity C-reactive protein (hs-CRP) indicates a high risk of inflammatory reactions. In clinical trials, internationally approved standardization is essential for obtaining study results that are comparable with those from overseas. Therefore, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and hs-CRP were standardized throughout a 10-year study period...
January 1, 2017: Annals of Clinical Biochemistry
Valentina Pecoraro, Giuseppe Banfi, Luca Germagnoli, Tommaso Trenti
BACKGROUND: Point of Care testing (POCT) has been developed to provide rapid test results. Most published studies focus on analytical performance, neglecting its impact on patient outcomes. OBJECTIVE: To review the analytical performance and accuracy of POCT specifically planned for immunoassay, and to evaluate the impact of faster results on patient management. METHODS: A search of electronic databases for studies reporting immunoassay results obtained in both POCT and central laboratory scenarios was performed...
January 1, 2017: Annals of Clinical Biochemistry
Mohamed Saleem, Helen Martin, Anne Tolya, Penny Coates
Background Interference from opiates in the Microgenics CEDIA(®) Buprenorphine assay is known to produce false positive buprenorphine screening immunoassay results necessitating confirmatory buprenorphine testing by chromatography/mass spectrometry methods. Method We reviewed data on falsely positive buprenorphine immunoassay screen (cut-off ≥ 5µg/L) but negative for buprenorphine by GCMS (cut-off ≥ 5µg/L) and had a positive opiate immunoassay result (cut-off ≥ 300µg/L). Results were collected over 3 months and the data were evaluated to determine whether there is an opiate immunoassay screen concentration below which a false positive buprenorphine result will not occur...
January 1, 2017: Annals of Clinical Biochemistry
Keiko Yasukawa, Tatsuo Shimosawa, Shigeo Okubo, Yutaka Yatomi
BACKGROUND: Human mercaptalbumin (HMA) and human nonmercaptalbumin (HNA) have been reported as markers for various pathological conditions, such as kidney and liver diseases. These markers play important roles in redox regulations throughout the body. Despite the recognition of these markers in various pathophysiologic conditions, measurements of HMA and HNA have not been popular because of the technical complexity and long measurement time of conventional methods. METHODS: Based on previous reports, we explored the optimal analytical conditions for a high-performance liquid chromatography (HPLC) method using an anion exchange column packed with a hydrophilic polyvinyl alcohol gel...
January 1, 2017: Annals of Clinical Biochemistry
Takashi Yamaguchi, Takeyoshi Murano, Ichiro Tatsuno, Nobuyuki Hiruta, Toru Suzuki, Shojiro Sawada, Hideki Katagiri, Kohji Shirai, Wolfgang J Schneider, Hideaki Bujo
BACKGROUND: We investigated in-vitro effects of various phospholipids as emulsifiers on the hydrolyzing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronemia, displays severely diminished activity for triolein when emulsified with Triton X-100. METHODS: Lipolytic activities of plasma obtained by heparin injection from a homozygous patient with LPL Arg243His were analyzed using as substrate triolein emulsified with phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), or Triton X-100...
January 1, 2017: Annals of Clinical Biochemistry
Ben Jones
No abstract text is available yet for this article.
January 1, 2017: Annals of Clinical Biochemistry
Jason Kc Mak
No abstract text is available yet for this article.
January 1, 2017: Annals of Clinical Biochemistry
Eriko Mori, Makoto Kurano, Akiko Tobita, Hironori Shimosaka, Yutaka Yatomi
Background Squamous cell carcinoma antigen is used as a tumour marker and is routinely measured in clinical laboratories. We validated two different immunoassays and found three cases in which the squamous cell carcinoma antigen concentrations deviated greatly between the two immunoassays. Here, we aimed to elucidate the mechanisms responsible for these deviations. Methods The squamous cell carcinoma antigen concentrations were determined using the ARCHITECT SCC (CLIA method) and the ST AIA-PACK SCC (FEIA method)...
January 1, 2017: Annals of Clinical Biochemistry
Mary Stapleton, Niamh Daly, Ruth O'Kelly, Michael J Turner
Background The inhibition of glycolysis prior to glucose measurement is an important consideration when interpreting glucose tolerance tests. This is particularly important in gestational diabetes mellitus where prompt diagnosis and treatment is essential. A study was planned to investigate the effect of preservatives and temperature on glycolysis. Methods Blood samples for glucose were obtained from consented females. Lithium heparin and fluoride-EDTA samples transported rapidly in ice slurry to the laboratory were analysed for glucose concentration and then held either in ice slurry or at room temperature for varying time intervals...
January 1, 2017: Annals of Clinical Biochemistry
Julian H Barth, Ahai Luvai, Nuthar Jassam, Wycliffe Mbagaya, Eric Stephen Kilpatrick, Deepa Narayanan, Shirley Spoors
Introduction Reference intervals are dependent on the reference population, the analytical methods and the way the data is handled statistically. Individual method related differences have been studied but the comparative differences in reference intervals have not. METHODS: We studied a reference population of healthy adult subjects and measured free thyroxine and TSH by the four most commonly used analytical platforms used in the UK. Subjects were excluded if they were >65 years or had positive thyroid peroxidase antibodies...
January 1, 2017: Annals of Clinical Biochemistry
Hiroyuki Teraura, Kazuhiko Kotani, Takaomi Minami, Taro Takeshima, Osamu Shimooki, Eiji Kajii
Kawasaki disease is a febrile disease of childhood that is associated with increased inflammatory cytokines and immunoregulatory abnormalities. While the serum concentrations of soluble IL-2 receptor can change under such pathologies, the relevance of the soluble IL-2 receptor concentration in patients with Kawasaki disease has not been specified. We aimed to summarize the existing studies that reported the soluble IL-2 receptor concentrations in patients with Kawasaki disease. Original articles that were published up to July 2016 were collected using a PubMed/Medline-based search engine...
January 1, 2017: Annals of Clinical Biochemistry
Yutaka Suehiro, Yibo Zhang, Shinichi Hashimoto, Taro Takami, Shingo Higaki, Yoshitaro Shindo, Nobuaki Suzuki, Shoichi Hazama, Masaaki Oka, Hiroaki Nagano, Isao Sakaida, Takahiro Yamasaki
BACKGROUND: As TWIST1 methylation is specific to colorectal neoplasia, detection of TWIST1 methylation from faeces samples might be useful for colorectal neoplasia screening. However, because the content of human DNA in faeces is very small, it is very difficult to detect TWIST1 methylation by conventional bisulphite-based methylation assays. Therefore, we developed a new methylation assay without bisulphite treatment, the combined restriction digital PCR (CORD) assay, and evaluated its sensitivity and specificity in combination with and without the faecal immunochemical test for haemoglobin (FIT) for colorectal neoplasia detection from faeces samples...
January 1, 2017: Annals of Clinical Biochemistry
Anna M Milan, Andrew Hughes, Andrew Stuart Davison, Jean M Devine, Jeannette L Usher, Sarah Curtis, Milad Khedr, James A Gallagher, L Ranganath
Background Alkaptonuria (AKU) is a rare, debilitating autosomal recessive disorder affecting tyrosine metabolism. Deficiency of homogentisate 1,2-dioxygenase leads to increased homogentisic acid (HGA) which is deposited as ochronotic pigment. Clinical sequelae include severe early onset osteoarthritis, increased renal and prostate stone formation and cardiac complications. Treatment has been largely based on analgaesia and arthroplasty. The National AKU Centre in Liverpool has been using 2mg nitisinone (NTBC) off-license for all patients in the United Kingdom with AKU and monitoring the tyrosine metabolite profiles...
January 1, 2017: Annals of Clinical Biochemistry
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