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Annals of Clinical Biochemistry

Saliha Mohammed Ismail Haji, Allison Chipchase, W Fraser, Javier Gomez
BACKGROUND: Alkaline phosphatase (ALP) isoenzyme analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally-derived screening algorithm for ALP isoenzyme requests on the number of ALP isoenzyme analyses performed, laboratory cost, and patient care. METHOD: 110 ALP isoenzyme analysis requests from the year 2015 were reviewed and subsequent ALP concentrations were monitored over a 2-year period, to determine if the decision of performing/not performing ALP isoenzyme analysis, based on the algorithm, had an impact on patient care and laboratory cost...
November 17, 2018: Annals of Clinical Biochemistry
Anne Sophie Bargnoux, Vincent Azoury, Stephanie Badiou, Kada Klouche, Maëlle Plawecki, Nils Kuster, Anne Marie Dupuy, Jean-Paul Cristol
BACKGROUND: The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction. METHODS: CysC in urine was measured by a particle enhanced nephelometric immunoassay (PENIA) using Siemens reagents and by a particle enhanced turbidimetric immunoassay (PENIA) using DiaSys reagents...
November 14, 2018: Annals of Clinical Biochemistry
G Woodward, R Pryke, B Hoppe, G Rumsby
BACKGROUND: The primary hyperoxalurias are inherited disorders of glyoxylate metabolism that lead to overproduction of oxalate, urolithiasis and renal failure. Delays in diagnosis can be costly in terms of preserving renal function. Here we present a rapid liquid chromatography tandem mass-spectrometry screening method for the analysis of metabolites (primary hyperoxaluria metabolites) produced in excess by primary hyperoxaluria patients that include glycolate, glycerate and 2,4-dihydroxyglutarate...
November 14, 2018: Annals of Clinical Biochemistry
Soheil Madadi, Meysam Soleimani
No abstract text is available yet for this article.
November 8, 2018: Annals of Clinical Biochemistry
James John Logie, Christopher Chaloner
BACKGROUND: Specimen contamination that goes unnoticed can have many adverse consequences for patients including inappropriate investigations or treatment decisions based on erroneous results. Little is known about UK laboratory practices relating to specimen contamination therefore this national survey aimed to gather valuable baseline data. METHODS: An electronic survey consisting of 26 questions was designed to obtain key information relating to specimen contamination including its frequency, how it is indentified by laboratories, and actions taken in event of confirmed contamination...
October 29, 2018: Annals of Clinical Biochemistry
Ross M Valentine, Hayley Sharrod-Cole, Clare Ford, Rousseau Gama
No abstract text is available yet for this article.
October 29, 2018: Annals of Clinical Biochemistry
Michael Thompson, Venkat Parameswaran, John Burgess
Background Patients with mutations of succinate dehydrogenase B (SDHB) and D (SDHD) are at high risk of paraganglioma (PGL) necessitating surveillance. Chromogranin A (CgA) has been proposed as a biochemical marker of PGL. We sought to determine the diagnostic utility of CgA in a population based SDHx sample. Methods Tasmania is an island state with one tertiary referral centre for endocrine neoplasia. We performed cross sectional analysis of all adult SDHB ( n=52) and SDHD ( n=10) patients undergoing PGL surveillance between 2011 and 2017...
October 29, 2018: Annals of Clinical Biochemistry
Li Ai, Jun Wang, Chang Zhao, Yonghe Tang, Weishan Wang, Yan Yu, Shengjie Zhao, Yalan Di, Xuechao Fei, Hongjun Luo, Hui Li, Wenhong Luo, Tingting Li, Rongqiao He, Weiying Lin, Zhiqian Tong
BACKGROUND: Morning urine formaldehyde (FA) levels could predict the severe degree of dementia in patients with post-stroke dementia (PSD) and Alzheimer's disease (AD). However, the routinely available technique of high-performance liquid chromatography (HPLC) for detecting urine FA requires expensive and sophisticated equipments. METHODS: We established a fluorescence spectrophotometric method by using a FA-specific fluorescent probe- NaFA (λex/em = 430/543 nm)...
October 29, 2018: Annals of Clinical Biochemistry
William A Sadler
BACKGROUND: Bland-Altman analysis is a popular and widely used method for assessing the level of agreement between two analytical methods. An important assumption is that paired method differences exhibit approximately constant (homogeneous) scatter when plotted against pair means. This allows estimation of limits of agreement which retain validity across the entire range of mean values. In practice, pair differences often increase systematically with the mean and Bland and Altman used log transformed data to achieve approximately homogeneous scatter...
October 29, 2018: Annals of Clinical Biochemistry
Asako Sato, Sayo Yada, Emiko Hosoba, Hiroko Kanno, Hitomi Miura
Background To promote glycated albumin (GA) standardization, Committee on Diabetes Mellitus Indices, Japan Society of Clinical Chemistry published the recommended reference method, and is supplying the reference standard material (JCCRM 611). In this study, we evaluated the basic performance of "standardized enzymatic method" that is traceable to JCCRM 611, and established a unit conversion formula from standardized GA value (mmol/mol) to the routinely used GA value (%) . Methods To evaluate accuracy and within-run reproducibility of the "standardized enzymatic method", JCCRM 611 was measured 20 times...
October 3, 2018: Annals of Clinical Biochemistry
Brian Shine, Julian H Barth
No abstract text is available yet for this article.
September 12, 2018: Annals of Clinical Biochemistry
Gautam Das, Peter N Taylor, Hussam Abusahmin, Amer Ali, Brian P Tennant, John Geen, Onyebuchi Okosieme
Background Microalbuminuria represents vascular and endothelial dysfunction. Thyroid hormones can influence urine albumin excretion as it exerts crucial effects on the kidney and on the vascular system. This study explores the relationship between serum thyrotropin and urine albumin excretion in euthyroid patients with diabetes. Methods A total of 433 patients with type 1 or 2 diabetes were included in this retrospective cross-sectional study. Data included anthropometric measurements and biochemical parameters from diabetes clinic...
September 11, 2018: Annals of Clinical Biochemistry
Ramin Ghodsi, Sorayya Kheirouri, Rahmat Nosrati
Background Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipeptide with antiglycation effects. The aim of this trial was to examine the effects of carnosine supplementation on the advanced glycation end products and the precursors of advanced lipoxidation end products in patients with autism...
September 6, 2018: Annals of Clinical Biochemistry
Zhen Chen, Yue Wu, Rojeet Shrestha, Zijun Gao, Yaoyao Zhao, Yusuke Miura, Akiko Tamakoshi, Hitoshi Chiba, Shu-Ping Hui
BACKGROUND: Short-chain fatty acid (SCFA) are primarily absorbed through the portal vein during lipid digestion, which is utilized as the energy source, as well as prevents type 2 diabetes and some cancers. However, reports on the determination of these SCFA in human serum are limited. METHODS: Blood samples from human subjects (n = 547, male/female = 246/301, age 58.85 ± 12.57) were collected. Saponification was applied to obtain total FA. After derivatization by 2-nitrophenylhydrazine, FA 4:0 and FA 6:0 were measured by liquid chromatography-mass spectrometry (LC-MS/MS)...
September 5, 2018: Annals of Clinical Biochemistry
Michael J Duffy
Measurement of genetically altered DNA shed from tumours into the circulation can potentially provide a new generation of blood-based cancer biomarkers. Compared with tissue DNA biomarkers which require surgery or biopsy, samples for circulating tumour DNA assays can be obtained with minimal inconvenience and at lower cost. Furthermore, in contrast to tissue, the use of circulating tumour DNA allows serial monitoring, faster delivery of results and potentially provides an integrative representation of genetic alterations across all tumour sites within a patient...
September 5, 2018: Annals of Clinical Biochemistry
Yu-Hung Chang, Hsien-Chang Lin, Der-Wei Hwu, Dao-Ming Chang, Kun-Chen Lin, Yau-Jiunn Lee
Background Serum cytokeratin-18 is believed to be a marker of hepatic cell damage. However, few studies have discussed about the serum cytokeratin-18 concentration in type 2 diabetes mellitus patients and investigated its association with non-alcoholic fatty liver disease as well as metabolic biomarkers. Methods Healthy participants and type 2 diabetes mellitus patients were enrolled. Physical and metabolic factors were recorded, and non-alcoholic fatty liver disease was screened by abdominal ultrasound and the fatty liver index...
August 27, 2018: Annals of Clinical Biochemistry
Alison Avenell, Mark J Bolland, Andrew Grey
No abstract text is available yet for this article.
August 27, 2018: Annals of Clinical Biochemistry
Guojun Zhang, Chunjiao Yang, Xixiong Kang, Zhixian Gao, Hong Wan, Yunpeng Liu
Background The differential diagnosis between postneurosurgical bacterial meningitis and aseptic meningitis remains challenging both for the clinician and the laboratory. Combinations of markers, as opposed to single ones, may improve diagnosis and thereby survival. Methods This prospective cohort study included patients with suspected bacterial meningitis after neurosurgery. The patients were divided into two groups according to the diagnostic criteria of meningitis involving a postneurosurgical bacterial meningitis group and a postneurosurgical aseptic meningitis group...
August 27, 2018: Annals of Clinical Biochemistry
H A Kingma, F H van der Sluijs, M R Heiner-Fokkema
Background Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focusing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. We investigated the Capillarys™ CDT assay (Sebia, France) to screen for N-glycosylation disorders, using IEF as gold standard. Methods Intra- and inter-assay precision were established, and analyses in heparin-anticoagulated plasma and serum were compared...
November 2018: Annals of Clinical Biochemistry
Michelle Ma Kip, Annemarie Schop, Karlijn Stouten, Soraya Dekker, Geert-Jan Dinant, Hendrik Koffijberg, Patrick Je Bindels, Maarten J IJzerman, Mark-David Levin, Ron Kusters
Background Establishing the underlying cause of anaemia in general practice is a diagnostic challenge. Currently, general practitioners individually determine which laboratory tests to request (routine work-up) in order to diagnose the underlying cause. However, an extensive work-up (consisting of 14 tests) increases the proportion of patients correctly diagnosed. This study investigates the cost-effectiveness of this extensive work-up. Methods A decision-analytic model was developed, incorporating all societal costs from the moment a patient presents to a general practitioner with symptoms suggestive of anaemia (aged ≥ 50 years), until the patient was (correctly) diagnosed and treated in primary care, or referred to (and diagnosed in) secondary care...
November 2018: Annals of Clinical Biochemistry
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