journal
MENU ▼
Read by QxMD icon Read
search

Scandinavian Journal of Immunology

journal
https://www.readbyqxmd.com/read/28891256/composition-and-variation-analysis-of-the-tcr-%C3%AE-chain-cdr3-repertoire-in-neonatal-sepsis
#1
Jinqiao Sun, Bijun Sun, Yanyan Gao, Fusheng He, Lin Yang, Mingbang Wang, Wenhao Zhou
T cell receptor (TCR) diversity is clearly related to protection from infection. However, the characteristics of TCR diversity in neonates are not clear. In this study, we investigated the TCR diversity of neonates with sepsis. Twenty neonates with severe sepsis and 8 matched neonates without infection were enrolled in the study. For the neonates with sepsis, EDTA-anticoagulated blood was collected on day 1 after the diagnosis of sepsis and on day 7 of treatment. For the neonates without infection, blood was collected one time...
September 11, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28889482/tlr-induced-smpd3-defects-enhance-inflammatory-response-of-b-cell-and-macrophage-in-the-pathogenesis-of-sle
#2
Fei Liu, Xiaojing Li, Huimin Yue, Jianjian Ji, Ming You, Liang Ding, Hongye Fan, Yayi Hou
B lymphocyte and macrophages may contribute to SLE pathogenesis through cytokine production after TLR stimulation. Emerging evidences suggested that defects of sphingolipid metabolism was responsible for SLE pathogenesis. However, it is not clear whether these defects exist in B cells and macrophages under SLE condition and whether TLR signaling pathway was related to the dysfunction of sphingolipid metabolism in SLE. Here we demonstrated that the enzymes involved in the sphingolipid metabolism expressed abnormally in B cells from SLE patients and lupus-prone mice...
September 10, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28888058/increased-expression-of-programmed-cell-death-1-in-regulatory-t-cells-of-patients-with-severe-sepsis-and-septic-shock-an-observational-clinical-study
#3
Qiang Liu, Le An, Zhijiang Qi, Yongzhen Zhao, Chunsheng Li
While regulatory T-cells (Tregs) constitutively express programmed cell death-1 (PD-1), the role of PD-1 expression in Tregs of patients with sepsis remains unclear. Thus, we determined PD-1 expression in Tregs from the peripheral blood of patients with severe sepsis and septic shock Seventy-eight patients with severe sepsis and 40 with septic shock, as well as 21 healthy subjects, were enrolled in this study. The percentage of peripheral blood PD-1+ Tregs, as well as absolute Treg counts, were compared between these 3 groups...
September 9, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28888053/mesenchymal-stem-cells-overexpressing-interleukin-35-propagate-immunosuppressive-effects-in-mice
#4
Hao Guo, Na Zhao, Haopeng Gao, Xianghui He
OBJECTIVE: To explore generation of interleukin (IL)-35-expressing mouse adipocyte-derived mesenchymal stem cells (Ad-MSCs) using lentiviral vector and their potential immunosuppressive effects in mice. METHODS: Ad-MSCs were isolated and cultured in vitro and transfected with a lentivirus vector for overexpression of the therapeutic murine IL-35 gene. IL-35 expression in transfected MSCs (IL-35-MSCs) was quantified by enzyme-linked immunosorbent assay (ELISA). The lymphocytes subsets after one-way mixed lymphocyte culture and in vivo intravenous transplantation were analyzed by flow cytometry to evaluate the immunosuppressive effects of IL-35-MSCs...
September 9, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28853768/systemic-treatment-with-a-mir-146a-mimic-suppresses-endotoxin-sensitivity-and-partially-protects-mice-from-the-progression-of-acute-graft-versus-host-disease
#5
John G Gartner, Melanie M Durston, Stephanie A Booth, Cynthia A Ellison
Acute GVHD (aGVHD) is driven by interactions between the allogenic T cell response, inflammation, tissue injury and microbial products that enter the circulation when protective barriers such as the intestinal epithelium become compromised. Mice with aGVHD become hypersensitive to LPS, secreting large quantities of inflammatory mediators that exacerbate tissue injury. We hypothesized that microRNA (miR) modulators could be used in vivo to mitigate LPS hypersensitivity, altering the course of aGVHD. Using the C57BL/6 → (C57BL/6 x DBA/2)F1-hybrid model of aGVHD, we measured intestinal permeability over time and used a qPCR array to detect concomitant changes in the expression levels of certain microRNAs (miRs) in the intestine...
August 29, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28817184/the-influence-of-cytomegalovirus-on-expression-of-hla-g-and-its-ligand-kir2dl4-by-human-peripheral-blood-leucocyte-subsets
#6
Zaid Albayati, Ahmed Alyami, Suliman Alomar, Derek Middleton, Laura Bonnett, Sobia Aleem, Brian F Flanagan, Stephen E Christmas
HLA-G is a non-classical class I HLA antigen, normally expressed in high levels only on extravillous cytotrophoblast. It has immunosuppressive properties in pregnancy and has also been found to be upregulated on leucocytes in viral infection. In this study, proportions of all leucocyte subsets expressing HLA-G were found to be low in healthy subjects positive or negative for cytomegalovirus (CMV). Significantly greater proportions of CD4+CD69+ and CD56+ T cells expressed HLA-G compared to other T cells. However, following stimulation with CMV antigens or intact CMV, proportions of CD4+, CD8+, CD69+ and CD56+ T cells, and also B cells expressing HLA-G were significantly increased in CMV+ subjects...
August 17, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28805315/a-comparison-of-clinical-and-immunologic-phenotypes-in-familial-and-sporadic-forms-of-common-variable-immunodeficiency
#7
Amir Valizadeh, Reza Yazdani, Gholamreza Azizi, Hassan Abolhassani, Asghar Aghamohammadi
Common variable immunodeficiency (CVID) is the most frequent symptomatic primary immunodeficiency disease and its prevalence varies significantly among different population. Minority of CVID patients present a familial aggregation suggesting a higher probability of heritable genetic defects. A total of 235 registered CVID patients were evaluated in this cohort study. Familial and sporadic patients were stratified and demographic information, clinical records, laboratory and molecular data were compared among these two groups of patients...
August 11, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28805301/the-lymphocytes-stimulation-induced-dna-release-a-phenomenon-similar-to-netosis
#8
Yermis Carolina Rocha, Mauricio Rojas, Gloria Vásquez, Juan López
The release of DNA into the extracellular milieu by neutrophil during a process called NETosis has been postulated as an additional source of autoantigens; a process believed to be important in the pathogenesis of some autoimmune disease, such as Systemic Lupus Erythematosus (SLE). However, it is not established if the B and T cells undergo the release of DNA to the extracellular milleu, in response to different stimuli. In this study, it was observed that, the treatment of B and T cells with PMA, ionomycin, and the serum from patients with SLE induced the extracellular DNA presence in B and T cells...
August 11, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28799233/stochastics-of-cellular-differentiation-explained-by-epigenetics-the-case-of-t-cell-differentiation-and-functional-plasticity
#9
REVIEW
Jaydeep Bhat, Johannes Helmuth, Guranda Chitadze, Léonce Kouakanou, Christian Peters, Martin Vingron, Ole Ammerpohl, Dieter Kabelitz
Epigenetic marks including histone modifications and DNA methylation are associated to the regulation of gene expression and activity. In addition, an increasing number of non-coding RNAs with regulatory activity on gene expression have been identified. Alongside, technological advancements allow for the analysis of these mechanisms with high resolution - up to the single-cell level. For instance, the assay for transposase-accessible chromatin using sequencing (ATAC-seq) simultaneously probes for chromatin accessibility and nucleosome positioning...
August 10, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28736829/clonal-analysis-of-regulatory-t-cell-defect-in-patients-with-autoimmune-polyendocrine-syndrome-type-1-suggests-intrathymic-impairment
#10
Tuisku-Tuulia Koivula, Sini M Laakso, Heikki J Niemi, Eliisa Kekäläinen, Pia Laine, Lars Paulin, Petri Auvinen, T Petteri Arstila
Mutations in the Autoimmune Regulator (AIRE) gene disrupt thymic T cell development and negative selection, leading to the recessively inherited polyendocrine autoimmune disease APS-1 (autoimmune polyendocrine syndrome type 1). The patients also have a functional defect in the FOXP3(+) regulatory T cell population, but its origin is unclear. Here we have used T cell receptor sequencing to analyze the clonal relationship of major CD4(+) T cell subsets in 3 patients and 3 healthy controls. The naive regulatory T cells showed little overlap with helper T cell subsets, supporting divergence in the thymus...
July 22, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28727155/mucosal-associated-invariant-mait-cells-are-deficient-in-systemic-sclerosis
#11
Arsène Mekinian, Thibaut Mahevas, Mohamad Mohty, Vincent Jachiet, Sébastien Rivière, Olivier Fain, Béatrice Gaugler
OBJECTIVES: Systemic sclerosis (SSc) is an autoimmune disease, characterized by fibrosis of the skin and other organs, vascular impairment and deficient immune responses. Mucosal associated invariant T cells (MAIT) cells have been involved in various inflammatory and autoimmune diseases. The aim of this study was to determine the frequencies of MAIT cells in the blood of patients with systemic sclerosis (SSc), and to compare their distribution in the different types of SSc. PATIENTS AND METHODS: Blood samples from SSc patients and healthy controls were examined by flow cytometer to analyze the frequencies of MAIT and γδ T cells...
July 18, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28708284/cd20-specific-immunoligands-engaging-nkg2d-enhance-%C3%AE-%C3%AE-t-cell-mediated-lysis-of-lymphoma-cells
#12
Matthias Peipp, Daniela Wesch, Hans-Heinrich Oberg, Sebastian Lutz, Anja Muskulus, Jan G J van de Winkel, Paul W H I Parren, Renate Burger, Andreas Humpe, Dieter Kabelitz, Martin Gramatzki, Christian Kellner
Human γδ T cells are innate-like T cells which are able to kill a broad range of tumor cells and thus may have potential for cancer immunotherapy. The activating receptor natural killer group 2 member D (NKG2D) plays a key role in regulating immune responses driven by γδ T cells. Here, we explored whether recombinant immunoligands consisting of a CD20 single-chain fragment variable (scFv) linked to a NKG2D ligand, either MHC class I chain-related protein A (MICA) or UL16 binding protein 2 (ULBP2), could be employed to engage γδ T cells for tumor cell killing...
July 14, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28810080/non-specific-aberrant-expression-of-t-follicular-helper-and-regulatory-cells-with-age
#13
LETTER
Ashish Kumar Vyas, Ritu Khosla, Nirupma Trehanpati
We read with great interest the article by Guo et al. [1], which showed that increased percentage of circulating T follicular helper (cTFh) and circulating T follicular regulatory (cTFr) (cells in non-small cell lung cancer (NSCLC) patients could be correlated with its pathogenesis. The role of TFh cells has been described in different types of cancers such as hepatocellular carcinoma [2], breast cancer [3], and has also emerged as a key factor in the pathogenesis of viral related diseases such as HBV [4]...
July 5, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28675263/specific-and-cross-reactive-plasmablast-response-in-humans-after-primary-and-secondary-immunization-with-vi-capsular-polysaccharide-typhoid-vaccine
#14
Sari H Pakkanen, Jussi M Kantele, Lars Rombo, Anu Kantele
Secondary immunization with polysaccharide vaccines may imply a risk of hyporesponsiveness. Despite the wide use of typhoid Vi capsular polysaccharide vaccine, its potential tendency to hyporesponsiveness has been inadequately addressed. While previous studies have explored serum antibody responses, we applied a more sensitive approach, a single-cell assay for circulating plasmablasts, to compare primary and secondary responses. Twelve subjects received primary and booster doses of the Vi vaccine (Typherix(®)) at 30-37-month intervals...
July 3, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28675455/lps-induced-ex%C3%A2-vivo-cytokine-production-is-not-augmented-in-patients-with-von-hippel-lindau-disease
#15
LETTER
D Kiers, H J L M Timmers, J Gerretsen, P Pickkers, M Kox
No abstract text is available yet for this article.
September 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28675453/new-dancing-couple-pd-l1-and-microrna
#16
REVIEW
A Grenda, P Krawczyk
Lung cancer (LC) is the most common cause of cancer death in the world. A great challenge in treating NSCLC is the discovery of advanced, molecular tools to diagnose the disease in early stages as well as the development of immunotherapy. MicroRNAs are regulatory molecules (~20 nt in length) with the ability to regulate the expression of genes. The recently described PD-1 and PD-L1 molecules have great importance for potential use in immunotherapy of many cancers. These molecules are associated with immune checkpoints and provide an opportunity for the treatment of advanced NSCLC patients with synthetic monoclonal antibodies...
September 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28671723/giving-context-to-non-self-marker-theories-of-immune-responsiveness
#17
M Cohn
In 2001, long ago by modern standards, Matzinger changed the emphasis of her Danger theory from one that downplayed the self-non-self discrimination to one that concerned itself with the regulation of effector class. This gave her theory a measure of validity that the other non-self-marker theorists have yet to confront. However, precision and clarity are still lacking.
September 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28671713/complement-c5a-induced-changes-in-neutrophil-morphology-during-inflammation
#18
S Denk, R P Taylor, R Wiegner, E M Cook, M A Lindorfer, K Pfeiffer, S Paschke, T Eiseler, M Weiss, E Barth, J D Lambris, M Kalbitz, T Martin, H Barth, D A C Messerer, F Gebhard, M S Huber-Lang
The complement and neutrophil defence systems, as major components of innate immunity, are activated during inflammation and infection. For neutrophil migration to the inflamed region, we hypothesized that the complement activation product C5a induces significant changes in cellular morphology before chemotaxis. Exposure of human neutrophils to C5a dose- and time-dependently resulted in a rapid C5a receptor-1 (C5aR1)-dependent shape change, indicated by enhanced flow cytometric forward-scatter area values. Similar changes were observed after incubation with zymosan-activated serum and in blood neutrophils during murine sepsis, but not in mice lacking the C5aR1...
September 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28605050/the-abl-1-kinase-is-dispensable-for-nk-cell-inhibitory-signalling-and-is-not-involved-in-murine-nk-cell-education
#19
S Ganesan, T T Luu, B J Chambers, S Meinke, P Brodin, E Vivier, D M Wetzel, A J Koleske, N Kadri, P Höglund
Natural killer (NK) cell responsiveness in the mouse is determined in an education process guided by inhibitory Ly49 and NKG2A receptors binding to MHC class I molecules. It has been proposed that inhibitory signalling in human NK cells involves Abl-1 (c-Abl)-mediated phosphorylation of Crk, lowering NK cell function via disruption of a signalling complex including C3G and c-Cbl, suggesting that NK cell education might involve c-Abl. Mice deficient in c-Abl expression specifically in murine NK cells displayed normal inhibitory and activating receptor repertoires...
September 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28561325/assays-for-infliximab-drug-levels-and-antibodies-a%C3%A2-matter-of-scales-and-categories
#20
L I Bader, S M Solberg, S H Kaada, N Bolstad, D J Warren, S Gavasso, C G Gjesdal, C A Vedeler
Immunogenicity is a frequent cause of secondary non-response to tumour necrosis factor (TNF) inhibitors. Drug level measurement and detection of antidrug antibodies have been shown to be cost effective and clinically relevant, and a large number of assays are available for these purposes. It is, however, difficult to compare assays and translate results into clinical meaningful information due to different methodological approaches and a lack of assay standardization. We have analysed infliximab drug levels and antidrug antibodies in 107 patient samples using enzyme-linked immunoassays (ELISA), immunofluorometric assays (IFMA) and reporter-gene assays (RGA)...
September 2017: Scandinavian Journal of Immunology
journal
journal
22806
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"